KR101551240B1 - Cosmetic Composition for Skin Whitening Having Kaempferia Parviflora - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic composition comprising a black ginger extract, and more particularly, to a cosmetic composition for skin whitening derived from a natural product which has a free radical scavenging activity and a tyrosinase activity inhibiting ability through a black ginger extract .
The cosmetic composition for whitening skin of the present invention comprises black ginger ( kaempferia parviflora extract as an active ingredient.
The cosmetic composition comprising the black ginger extract of the present invention does not cause skin irritation by using a natural extract and protects the skin from external stimuli to make the skin soft and radiant, provides excellent whitening effect and antioxidant, It is very effective for improvement and skin protection.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cosmetic composition for skin whitening comprising a black ginger extract (Cosmetic Composition for Skin Whitening Having Kaempferia Parviflora)

The present invention relates to a skin whitening cosmetic composition comprising a black ginger extract, and more particularly, to a cosmetic composition for skin whitening derived from a natural product which has a free radical scavenging activity and a tyrosinase activity inhibiting ability through a black ginger extract .

Today, oriental women prefer white, clean skin like white whiting and use it as an important criterion of beauty. Therefore, whitening agents are actively being developed to treat skin pigmentation abnormalities and meet beauty needs.

The skin color of a person depends on internal and external factors. Among them, melanin, the natural skin pigment, is the most fundamental internal color. Melanin is synthesized in the melanocyte, a pigment cell in the basal layer of the epidermis. This melanocyte is a nerve-derived cell, and it plays an important role in protecting the skin from ultraviolet rays by generating melanin.

Melanocytes are morphologically similar to dendritic cells with dendritic spines, and have many receptors for many signaling and growth factors, and there are many features that indicate that the origin of the melanin is the same as neurons.

Melanocytes are affected by various factors such as ultraviolet rays, external conditions such as inflammation, hormones such as α-MSH, cytokines such as IL-1, TNF-α and GM-CSG, and leukotrienes. In addition, in order for melanin to be produced from melanocytes, several kinds of genes are involved in the action of various genes such as tyrosinase, TRP-1 and TRP-2 and their enzymes, and melanin migration into keratinocytes .

To briefly describe the formation of melanin, tyrosine is first converted to DOPAquinone via DOPA by an enzyme called tyrosinase in melanocyte endoplasmic reticulum in melanocyte, (DOPAchrome) through enzymatic reaction and autoxidation reaction to produce melanin as a dark brown copolymer. The resulting melanin is transferred to the keratinocyte through melanoma, and after about 28 days of keratinization, it exits the skin surface and disappears with the keratinocytes.

In the development of a whitening agent for preventing melanin pigment deposition, there is known a method of decolorizing by reducing the melanin pigment presently produced and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. However, it is known that whitening agents using tocopherol, vitamins and the like, which are used for reducing the melanin pigment, have little discoloring effect on the melanin pigment, and inhibitors that inhibit the activity of tyrosinase have been attracting attention .

Free radicals and reactive oxygen species, which are highly reactive in skin aging, are common causes of extrinsic aging and endogenous aging. As a person gets older, antioxidant enzymes such as antioxidant enzymes and vitamin E, C, glutathione, and ubiquinol are reduced in the body, resulting in an imbalance of oxidation and antioxidation, thereby promoting the melanin production reaction, , Which causes skin elasticity, wrinkles and stains, and exogenous skin aging such as freckle formation. Therefore, a whitening composition that inhibits the activity of tyrosinase and has an antioxidative effect can more effectively induce a skin whitening effect.

In the conventional cosmetics field, as a whitening ingredient, for example, substances inhibiting tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, L-ascorbic acid ), Derivatives thereof and various plant extracts have been used. However, since the substance inhibiting the tyrosinase enzyme activity is low in stability in the prescription system, it is decomposed and colored, its use is limited due to the generation of offensive odor, efficacy at the living body level, uncertainty of the effect, It is true.

On the other hand, the black ginger ( kaempferia parviflora Wall. Ex Baker ) is a root-tinged plant of the native plant of Thailand . When cut immediately after harvesting, it shows purple or black inside. The black ginger has been known to help maintain mental and physical stability and stable blood pressure, to treat dysentery and to treat abdominal pain.

However, it has not yet been reported that black ginger has a skin whitening effect.

As a result, the inventors of the present invention have found that black ginger exhibits excellent whitening effect through its ability to inhibit free radical scavenging activity and tyrosinase activity, thereby completing the present invention.

An object of the present invention is to provide a cosmetic composition for skin whitening comprising a black ginger extract having a free radical scavenging activity and inhibiting tyrosinase activity so that melanin pigment deposition can be effectively prevented or solved.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening comprising an extract of black ginger ( kaempferia parviflora ) as an active ingredient.

In the present invention, the extract is characterized by being extracted with water, an organic solvent or a mixed solvent thereof.

In the present invention, the organic solvent is selected from the group consisting of methanol, ethanol, butanol, hexane, ether, benzene, chloroform and ethyl acetate.

In the present invention, the composition Daechun raw (Nicandra physaloides (L.) Gaertn. ), Abutilon theophrasti Medicus , Achillea alpina there is . discoidea (Regel) Kitam. And Amorpha fruticosa L. ). ≪ / RTI >

In the present invention, the composition has a skin whitening effect through inhibition of free radical scavenging and tyrosinase activity.

In the present invention, the composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a nutrient lotion, a milk lotion, a moisturizing lotion, a nutrition cream, a massage cream, a moisturizer cream, Soaps, cleansing foams, cleansing lotions, cleansing creams, body cleansers and body lotions.

The cosmetic composition comprising the black ginger extract of the present invention does not cause skin irritation by using a natural extract and protects the skin from external stimuli to make the skin soft and radiant, provides excellent whitening effect and antioxidant, It is very effective for improvement and skin protection.

1 is a photograph of black ginger according to the present invention.

Hereinafter, the present invention will be described more specifically.

In the present invention, 50 kinds of plants were extracted with water and an organic solvent in order to find a new plant whose whitening activity was not known, and the free radical scavenging ability and tyrosinase activity inhibitory ability of the extracted plant extract were examined. As a result, black with ginger (kaempferia parviflora), Saint Daecheon (Nicandra physaloides (L.) Gaertn . ), Abutilon theophrasti Medicus , Achillea ( Achillea alpina there is . discoidea ( Regel ) Kitam . ) And Amorpha fruticosa L. showed excellent free radical scavenging ability and inhibition of tyrosinase activity.

The present invention dark ginger (kaempferia parviflora extract as an active ingredient.

The cosmetic compositions of the present invention, black ginger (kaempferia with respect to the total weight of the cosmetic composition parviflora extract may be contained in an amount of 0.1 to 20% by weight, preferably 1 to 10% by weight.

The black ginger ( kaempferia parviflora extract is contained within the above ranges, a significant effect can be obtained. When the final product is prepared, it is preferable to include the above-mentioned content in consideration of the intrinsic odor and color of the extract.

The method for obtaining the above extract may be any method conventionally used in the art, but it is preferable to use black ginger ( kaempferia parviflora ) is preferably extracted with water, an organic solvent or a mixed solvent thereof.

The organic solvent was black ginger ( kaempferia parviflora , and any solvent which is not toxic can be used without limitation, and examples thereof include methanol, ethanol, butanol, hexane, ether, benzene, chloroform, ethyl acetate and the like.

When the extract of the present invention is prepared, the extraction solvent may be used alone or in combination with a dried black ginger kaempferia parviflora is used in an amount of 150 to 300 parts by weight, but not limited thereto.

In the present invention, the cosmetic composition for whitening skin is selected from the group consisting of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus , Achillea alpina var. Discoidea (Regel) Kitam. Amorpha fruticosa L. ). ≪ / RTI >

The part of the plant is not particularly limited, but Nicandra physaloides (L.) Gaertn. Is a fruit, leaf, stem; Abutilon theophrasti Medicus is a leaf, flower, seed; Achillea alpina var. Discoidea (Regel) Kitam. ) Is the root, stem; It is preferable to use the flower part of the weevi sari ( Amorpha fruticosa L. ).

The above extracts of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus , Achillea alpina var. Discoidea (Regel) Kitam or Amorpha fruticosa L. also have free radical scavenging ability or Since it has inhibitory activity against tyrosinase, black ginger ( kaempferia parviflora extract can be used to enhance the skin whitening effect due to the synergy effect.

The above- mentioned extracts of Nicandra physaloides (L.) Gaertn. , Abutilon theophrasti Medicus , Achillea alpina var. Discoidea (Regel) Kitam or Amorpha fruticosa L. , And may be contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition.

In the present invention, the skin whitening cosmetic composition may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a nutrition lotion, a milk lotion, a moisturizing lotion, a nutritional cream, a massage cream, But it is not limited thereto and may be prepared in any formulations selected from the group consisting of essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body cleanser and body lotion. Can also be produced. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

The cosmetic composition of the present invention may be blended with other components to be added to cosmetics conventionally used as needed.

The cosmetic composition according to the present invention may be used as an additive in the form of an additive such as a preservative, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, The composition may further comprise one or a mixture of two or more members selected from the group consisting of cold agents, antiperspirants, stabilizers, solubilizers, fragrances and purified water, but is not limited thereto. .

Hereinafter, the present invention will be described in more detail by way of examples. The following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by these examples.

[Example 1] Preparation of extract

The dried ginger, Daechunbyeol, the goat, the grasshopper and the weevil broth dried in the Kyungdong medicinal product market were purchased, and 2 kg of the dried product was uniformly ground. Then, the extract obtained by immersing in 10 L of methanol for 2 days was filtered twice , And the filtrate was concentrated with a concentrator to obtain respective methanol extracts.

[Example 2] Antioxidative effect test of extract

The antioxidative effects of the methanol extracts of black ginger, arthropods, diarrhea, cauliflower and weevil syrup prepared in Example 1 were evaluated by the following tests.

2-1. DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity experiment

DPPH (1,1-diphenyl-2-picryhydrazyl) radical scavenging activity test is a widely used simple antioxidant detection method, and is a method widely used for the measurement of antioxidative activity of phenol and aromatic amines.

DPPH, a kind of dye, exhibits a strong absorption band at 525 nm due to the odd electrons it has. However, when reacting with an electron donor such as phenol or a hydrogen donor, electrons or hydrogen radicals are generated from the donor, and the absorption band disappears and becomes a stable molecule. In addition, the donated electrons are irreversibly bound, and the color of purple DPPH gradually decreases in proportion to the number, and the color of the reaction solution turns yellow. The radical scavenging activity can be determined by measuring the value of the absorbance thus decreasing.

The DPPH radical scavenging activity experiment was applied by Blois method. 100 μl of a 0.4 mM DPPH solution dissolved in methanol was added to a 96-well plate, and 100 μl of each of the samples was dissolved in methanol at each concentration. The mixture was uniformly mixed with a vortex mixer, and incubated in a dark room for 30 minutes And the absorbance was measured at 517 nm.

In the control group, methanol was added instead of the example, and methanol was added in place of the DPPH solution to obtain a correction value. As a comparative example, Butylatd Hydroxy Anisole (BHA) was used. The free radical scavenging ratio was calculated according to the following formula, and the results are shown in Table 1.

S: absorbance of the example / comparative example

SB: Absorbance of the Example / Comparative Example in which the buffer was substituted for the enzyme

C: Absorbance of the control group

division Extract concentration (/ ml) * SC50 7.82 15.63 31.25 62.50 125 250 500 Black ginger 38.21 54.21 86.76 92.12 93.02 94.01 98.89 9.08 Daecheon-bi 23.76 31.76 49.97 73.66 85.88 91.44 98.07 13.06 The 12.64 29.65 57.43 69.39 79.78 81.01 82.97 20.43 Mountain goat 11.04 30.01 61.88 73.00 76.31 79.21 83.94 38.21 Weasel 15.21 35.26 59.99 70.47 85.77 89.09 90.17 35.76 Black ginger + Daechan (5: 5) 32.00 43.55 54.76 68.60 80.00 89.51 92.34 12.01 Black ginger + rag (5: 5) 22.41 37.00 67.89 75.39 83.11 86.01 89.70 18.33 Black ginger + mountain goat (5: 5) 17.44 38.81 69.12 78.90 82.16 88.21 86.37 35.11 Black ginger +
The Weasel (5: 5) 18.22 39.21 64.47 73.78 84.00 94.09 95.17 30.60 Black ginger + Daeheon-ji + Arane + San weed + Weasel
(5: 1.25: 1.25: 1.25: 1.25) 28.51 35.06 42.49 65.69 79.11 81.31 86.78 13.59 BHA 10.41 32.00 56.89 68.01 81.65 86.71 89.03 34.12

*: SC50 is a concentration indicating 50% active inhibition.

From Table 1, it can be seen that the black ginger extract, the arthropod green extract and the goat extract exhibit higher free radical scavenging activity than the BHA (SC50 34.12 占 퐂 / ml), and the Acanthopanax scurvy and Ferret scarlet extracts are comparable to BHA (SC50 34.12 占 퐂 / Free radical scavenging activity.

Also, it was confirmed that when these were used in combination, they had a similar or similar radical scavenging activity than when they were used alone.

[Example 3] Tyrosinase activity inhibition experiment

The tyrosinase inhibitory activity of the extract was measured by Tomita et al.

The reaction solution contained 0.5 ml of 0.1 M phosphate buffer (pH 6.5), 50 μl of 0.05 M phosphate buffer (pH 6.5), mushroom tyrosinase (2,000 U / ml, Sigma Aldrich, USA) , 0.2 ml of 1.5 mM Ltyrosine (Acros, USA) and 0.1 ml of the Example. The reaction solution was added to a 1.5 ml microtube and reacted in a heating bath at 25 ° C for 10 minutes. After the reaction, the enzyme reaction was stopped for 5 minutes on ice, and the absorbance was measured at 470 nm using a microplate reader.

As a comparison, ascorbic acid and kojic acid were diluted with phosphate buffered saline. Concentrations of the extracts prepared in Example 1 and the comparative examples were calculated by diluting with a concentration range of 100, 250, 500 and 1000 ppm. 0.1 ml of phosphate buffered saline (PBS) was added instead of the solution prepared in Example 1 as a control. In addition, 50 μl of 0.1 M phosphate buffer (pH 6.5) was added instead of tyrosinase to prepare a blank. The inhibition rate (%) of enzyme activity was calculated as shown in Table 2 below.

A: Absorbance without addition of the Example / Comparative Example (control group)

B: Absorbance with addition of the example / comparative example

division Extract concentration (ppm) 100 250 500 1000 Black ginger -4.02 -5.02 5.02 46.02 Daecheon-bi -10.72 -4.83 -1.83 6.75 The -9.54 -3.32 2.19 10.72 Mountain goat -10.5 -4.9 -1.98 25 Weasel -8.54 -6.32 -1.19 28.72 Black ginger + Daechan (5: 5) -5.29 -1.33 8.73 31.56 Black ginger + rag (5: 5) -5.46 -1.12 5.99 33.26 Black ginger + mountain goat (5: 5) -6.57 -3.95 -1.88 31.5 Black ginger + weasel sari (5: 5) -3.44 3.32 7.45 32.22 Black ginger + Daeheon-ji + Arane + San weed + Weasel
(5: 1.25: 1.25: 1.25: 1.25) -2.5 1.8 16.21 43.65 ascorbic acid 12.21 27.01 60.94 93.1 kojic acid 71.23 88.11 93.88 96.1

From Table 2, the black ginger extract showed excellent tyrosinase inhibitory activity. It was also found that when these were used in combination, they exhibited similar or similar tyrosinase inhibitory activities than when they were used alone.

Therefore, the extracts prepared in Example 1 inhibited the synthesis of melanin by tyrosinase, and thus it is considered that they exhibit excellent whitening effect.

[Formulation Example 1] Flexible lotion

Formulation examples of the soft lotion containing the above Examples are shown in Table 3. < tb > < TABLE >

ingredient Content (% by weight) Black ginger methanol extract 10.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative 0.150 coloring agent 0.150 Flavoring agent 0.200 Purified water Balance total 100

[Formulation Example 2] Nourishing cream

Formulation examples of nutritional creams containing the above Examples are shown in Table 4. < tb > < TABLE >

ingredient Content (% by weight) Black ginger methanol extract 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Daechon-si methanol extract 5.0 Magnesium aluminum silicate 0.3 Stearic acid 2.0 Mineral oil 4.0 Squalene 3.8 Dimethicone 0.4 Dipotassium glycyrrhizate 0.05 Allantoin 0.05 Sodium hyaruronate 0.2 Tocopheryl acetate 0.2 Triethanolamine 0.2 Preservative 0.1 coloring agent 0.1 Flavoring agent 0.2 Purified water Balance total 100

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (6)

Black gem ( kaempferia parviflora ) extract; And Nicandra physaloides (L.) Gaertn. ) Extract or a mixture of black gem ( kaempferia parviflora ) extract; Nicandra physaloides (L.) Gaertn. Extract; Abutilon theophrasti Medicus extract; Achillea alpina var. Discoidea (Regel) Kitam. ) Extract; And Amorpha fruticosa L. extract, as an active ingredient, as an active ingredient.
The method according to claim 1,
Wherein the extract is extracted with water, an organic solvent, or a mixed solvent thereof, and a black ginger extract.
3. The method of claim 2,
Wherein the organic solvent is selected from the group consisting of methanol, ethanol, butanol, hexane, ether, benzene, chloroform and ethyl acetate.
delete The method according to claim 1,
Wherein the composition has a skin whitening effect through inhibition of free radical scavenging and tyrosinase activity.
The method according to claim 1,
The composition may be in the form of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a nutrition lotion, a milk lotion, a moisturizing lotion, a nutrition cream, a massage cream, a moisturizer cream, a hand cream, Wherein the composition has any one of the formulations selected from the group consisting of cleansing lotion, cleansing cream, body cleanser and body lotion.

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