KR100530669B1 - Cosmetic Compositions Comprising Lactate and Glycyrrhiza Uralensis Extracts for Skin Whitening - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition for skin whitening comprising 10-20% by weight lactate and 0.0001-15% by weight licorice extract as an active ingredient.

The cosmetic composition of the present invention exhibits an improved whitening effect with very low skin irritation.

Description

Cosmetic composition for skin whitening including lactate and licorice extract {Cosmetic Compositions Comprising Lactate and Glycyrrhiza Uralensis Extracts for Skin Whitening}

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition for skin whitening comprising lactate and licorice extract as active ingredients.

The color expressed in human skin, hair, eyes, and the like is determined by melanin, carotene and hemoglobin, and in particular, the most important factor determining skin color is melanin, and the color of skin depends on the amount, nature, and distribution of melanin. In humans the pigment of the skin or hair serves to protect against the harmful effects of sunlight, in particular ultraviolet light. For example, people with poor pigmentation are very sensitive to sunlight and are susceptible to burns and are at high risk of developing skin cancer even at a young age. Short wavelength ultraviolet (290-320 nm) and carcinogens form harmful oxygen radicals in the skin, which are known to attack skin cells and age the skin.

The removal of these harmful radicals to protect the skin from the damage caused by melanin is a major function. Thus, high melanin means that it has an effective response system to protect skin from physical and chemical toxic substances. Factors that promote the production of melanin include ultraviolet rays or hormones such as estrogen, prostaglandin and the like.

Melanin is produced by the complex oxidation and condensation reaction of melanocytes in the melanocytes by tyrosine is converted to dopa and dopaquinone by an enzyme called tyrosinase. The resulting melanin exhibits a circulating action that is delivered to the skin cells and is lost with the epidermal detachment and disappears.

On the other hand, when the skin responds to external stimuli such as ultraviolet rays, environmental pollution or stress, melanin overproduction occurs and pigmentation occurs when it remains in the skin without being discharged out of the skin. By preventing the activity of melanin by inhibiting the activity of tyrosinase, an enzyme involved in the production of melanin, it is possible to prevent such pigmentation and whitening effect can be expected.

There are many ways to suppress melanin production in whitening cosmetics. First, as a method of blocking and removing ultraviolet rays, which is the main cause of melanin production, this method can be expected to contain good light scattering agent or light blocking agent in the cosmetic composition. Second is a method of inhibiting melanin production by inhibiting the synthesis of core carbohydrates, such as glucosamine, required for the activity of tyrosinase. Third, a method of interfering with the function of tyrosinase by using an enzymatic acid such as kojic acid, or arbutin. Fourth, a method of preventing cell division using a substance having a specific toxicity against melanocytes, which are cells producing melanin, such as hydroquinone. Fifth, a method of using a substance, such as vitamin C, that exhibits a strong antioxidant effect during tyrosine through complex oxidation and condensation reactions through dopa and dopaquinone.

On the other hand, Japanese Patent Laid-Open No. 4-9320 discloses a whitening cosmetic using ascorbic acid. However, ascorbic acid has a problem in that the phase stability is poor in the prescription system and the activity of the active ingredient is inferior with time. Therefore, in recent years, a method of stabilizing using capsules and liposomes, etc. has been proposed, but no clear stabilization method has yet been proposed.

In addition, Japanese Patent Laid-Open No. 6-192062 discloses a whitening cosmetic using hydroquinone. However, hydroquinone has an excellent whitening effect but is limited to its use as a cosmetic since it is a carcinogenic substance.

In addition, Japanese Patent Laid-Open No. 56-7710 discloses a whitening cosmetic using kojic acid. Kojic acid has an excellent whitening effect due to its excellent activity of tyrosinase, an enzyme involved in the intermediate process of tyrosine to dopa and dopaquinone, but has a safety aspect such as browning in the prescription system.

In addition, Japanese Patent Laid-Open No. Hei 4-9315 discloses a whitening cosmetic using arbutin. Arbutin can be extracted or synthesized from alpine bilberry, which is a natural plant material. Like kojic acid, the inhibitory activity against tyrosinase has been demonstrated. However, since arbutin is a state in which sugar is attached to hydroquinone, sugar is separated by skin enzymes when applied as a cosmetic and there is a concern that skin irritation by hydroquinone is concerned.

Due to this problem in the related art, the development of whitening agent raw materials which improve safety using various natural products has been attempted.

Throughout this specification, numerous patent documents are referenced and their citations are indicated. The disclosures of the cited patent documents are incorporated herein by reference in their entirety, so that the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.

The present inventors have diligently researched to develop a cosmetic composition having higher skin safety and excellent whitening effect. As a result, when the lactate and licorice extract are used as active ingredients and the composition ratio is properly adjusted, the skin is synergistically exerted. By confirming that the stability is greatly improved, the present invention has been completed.

Accordingly, it is an object of the present invention to provide a whitening cosmetic composition comprising lactate and licorice extract.

Other objects and advantages of the invention will be apparent from the following examples and claims.

The present invention provides a cosmetic composition for skin whitening comprising 1-20% by weight of lactate and 0.0001-15.0% by weight of licorice extract as an active ingredient.

Lactate used as an active ingredient in the present invention is a component that suppresses the production of tyrosinase and shows a whitening effect, and has already been used in various kinds of cosmetics. In addition, lactate is a component that shows the effect of increasing the level of moisturizing effect and natural ceramide in the skin in addition to the whitening effect in the cosmetic composition.

The present inventors have conducted various studies to remove the side effects (e.g. skin irritation such as erythema, itching, etc.) caused by lactate used as a whitening ingredient and to improve the whitening effect. When used in combination, synergic whitening effect was observed and skin irritation was also greatly reduced.

The lactate and licorice extract used as active ingredients in the present invention are included in an amount of 1-20% by weight of lactate and 0.0001-15% by weight of licorice extract, based on the total cosmetic composition, preferably 10-15% by weight of lactate. % And licorice extract in an amount of 0.1-12% by weight.

When the content of lactate in the cosmetic composition of the present invention is less than 1% by weight, there is a problem in that the peeling effect is reduced, and when it exceeds 20% by weight, severe skin irritation is caused. In addition, when the content of licorice extract in the cosmetic composition of the present invention is less than 0.0001% by weight, there is a problem that does not exhibit a whitening effect, when it exceeds 15% by weight because no further increase in the whitening effect is expected 15% by weight It is not meaningful to add more, and some skin irritation may occur.

According to the most preferred embodiment of the present invention, the amount of sodium lactate is 15% by weight, the amount of the licorice extract is 10% by weight.

According to the most preferred embodiment of the present invention, the lactate included in the composition of the present invention is 60% (w / v) lactate.

The lactates of the present invention may take the form of salts in combination with various cations such as organic or inorganic cations. Preferably it can be combined with an inorganic cation to take the form of a salt, most preferably the lactate of the invention is sodium lactate. Sodium lactate is in the form of a salt and has a low molecular weight that facilitates penetration into the skin.

In addition, licorice ( Glycyrrhiza uralensis ) used as an active ingredient in the cosmetic composition of the present invention is a medicinal plant growing in northeastern China and throughout Asia as a perennial herb of legumes. The present inventors screened a substance that eliminates the side effects caused by lactate and improves the whitening effect using various natural materials, and found that the extract derived from licorice exhibits the above-mentioned activity.

Licorice extract used in the present invention can be obtained using various organs, tissues (for example, roots, leaves, flowers, stems, fruits and seeds, etc.) of licorice, and most preferably is an extract obtained from the root of licorice . The root of the licorice roots are reddish brown deep into the ground, the stems are gathered and grow straight about 1 m. In addition, the white hairs are dense and look white and scattered with dots. The root is sweet and is used a lot as a herbal medicine. Licorice contains a lot of glycyrrhizin, flavonoids, saponies, coumarins, tannins and enzymes to remove harmful oxygen produced by UV rays, prevent melanin photooxidation, and make the skin white and transparent.

The licorice extract of the present invention has a synergistic whitening effect when used in combination with lactate in an appropriate ratio and greatly reduces skin irritation.

The licorice extract of the present invention may be prepared using a variety of extraction solvents such as (a) water, (b) anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) A mixed solvent with water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol and (h) butyl acetate can be obtained from licorice. More preferably, the licorice extract of the present invention is obtained using a hydrous lower alcohol, most preferably ethanol. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also other licorice extracts having substantially the same effect.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The active fraction is also included in the licorice extract of the present invention.

Licorice extract of the present invention may be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

The lactate of the present invention exfoliates the keratin of the skin and at this time, licorice extract, which is a natural extract, penetrates into the skin while alleviating the irritation occurring in the stratum corneum, and a synergistic effect of the whitening effect appears.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to lactate and licorice extract as active ingredients, and include, for example, conventional agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Adjuvants, and carriers.

The whitening composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing It may be formulated as cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example

Example material

Licorice root of the present invention was purchased from Koryo Pharmaceutics Co., Ltd. (Korea, Cheonan) and used for extract preparation.

Example 1 Preparation of Extract of Licorice

100 g of licorice root was chopped and 100 ml of 95% hydrous ethanol was added to the dry weight. In an extractor equipped with a cooling condenser to prevent evaporation of the active ingredient, the mixture was heated to 50-100 ° C. for 10 hours, filtered, and the filtrate was concentrated under reduced pressure and dried to obtain 5.3 g of a powdery extract.

Example 2: Determination of Tyrosinase Inhibition of Licorice Extract

Tyrosinase inhibition of licorice extract was measured by concentration to determine the appropriate content of licorice extract to be added to the cosmetic composition of the present invention.

The reaction solution was prepared by adding licorice extract at various concentrations to 500 μl of 0.1 mg / ml tyrosine (Sigma) as a substrate. Sodium phosphate buffer (pH 6.8) was used as the buffer solution. 500 µl of tyrosinase (250 U / ml, Sigma) was added to the reaction solution, reacted at 37 ° C for 10 minutes, and the absorbance was measured at 475 nm. As a control, a licorice extract was not added.

Inhibition was calculated according to the following equation (1).

% Inhibition = (1-control absorbance (O.D.475) / control absorbance (O.D.475)) x 100

The experimental results are shown in Table 1.

Licorice Extract (wt%) Inhibition       0.0001 16.2        0.001 23.6         0.01 40.1          0.5 45.0            5 60.5           10 79.3           15 87.1           20 85.7           25 86.4 Control 0.0

As shown in Table 1, since the degree of tyrosinase inhibition is constant in the licorice extract 15% by weight or more, the proper amount of the licorice extract was determined to be 15% by weight.

Example 3: Preparation of Cosmetic Composition

In order to identify a suitable mixing ratio of sodium lactate and licorice extract in a whitening cosmetic composition, an example of a cosmetic composition containing sodium lactate and licorice extract mixed in various mixing ratios is prepared a flexible lotion, milk lotion, and nutrition cream. It was. However, the formulation of the present invention is not limited thereto.

Formulation Example 1 Flexible Lotion

To the flexible cosmetic lotion of Formulation Example 1 and Comparative Examples 1-3 with the composition of Table 2.

ingredient Content (% by weight) Formulation Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Licorice extract 10.0 - - 10.0 60% Sodium Lactate 15.0 - 15.0 - Tocopherol Acetate 5.2 5.2 5.2 5.2 Oleyl alcohol 1.5 1.5 1.5 1.5 ethanol 3.2 3.2 3.2 3.2 Plant extracts 1.0 1.0 1.0 1.0 Benzophenone-9 2.0 2.0 2.0 2.0 Carboxyl Vinyl Polymer 1.0 1.0 1.0 1.0 Cellulose gum 3.5 3.5 3.5 3.5 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount system 100 100 100 100

Formulation Example 2: Milk Lotion

The milk lotions of Formulation Example 2 and Comparative Examples 4-6 were prepared using the compositions shown in Table 3 below.

ingredient Content (% by weight) Formulation Example 2 Comparative Example 4 Comparative Example 5 Comparative Example 6 Licorice extract 10.0 - - 10.0 60% Sodium Lactate 15.0 - 15.0 - glycerin 5.1 5.1 5.1 5.1 Propylene glycol 4.2 4.2 4.2 4.2 Tocopheryl acetate 3.0 3.0 3.0 3.0 Floating paraffin 4.6 4.6 4.6 4.6 Triethanolamine 1.0 1.0 1.0 1.0 Squalane 3.1 3.1 3.1 3.1 Macadamia Nut Oil 2.5 2.5 2.5 2.5 Polysorbate 60 1.6 1.6 1.6 1.6 Sorbitan sesquirrolate 1.6 1.6 1.6 1.6 Propylparaben 0.6 0.6 0.6 0.6 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount system 100 100 100 100

Formulation Example 3: Nutrition Cream

The nutrition cream of Formulation Example 3 and Comparative Example 7-9 was prepared with the composition of Table 4 below.

ingredient Content (% by weight) Formulation Example 3 Comparative Example 7 Comparative Example 8 Comparative Example 9 Licorice extract 10 - - 10 60% Sodium Lactate 15.0 - 15.0 - glycerin 4.0 4.0 4.0 4.0 vaseline 3.5 3.5 3.5 3.5 Triethanolamine 2.1 2.1 2.1 2.1 Floating paraffin 5.3 5.3 5.3 5.3 Squalane 3.0 3.0 3.0 3.0 Beeswax 2.6 2.6 2.6 2.6 Tocopheryl acetate 5.4 5.4 5.4 5.4 Polysorbate 60 3.2 3.2 3.2 3.2 Carboxyl Vinyl Polymer 1.0 1.0 1.0 1.0 Sorbitan sesquioleate 3.1 3.1 3.1 3.1 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Remaining amount Remaining amount Remaining amount Remaining amount system 100 100 100 100

Experimental Example

Experimental Example 1: Skin irritation test

Fifty healthy men and women were tested for skin irritation of the products prepared according to the formulation and comparative examples. After a certain amount (approximately 0.1 g) of each sample (Formula 1-3 and Comparative Example 1-9) was patched for 24 hours at 5 x 20 cm 2 in the lower arm of each arm, each one was removed and 1 hour and 24 hours later. The skin condition change was read visually and the results are shown in Table 5. In addition, the skin irritation rate was calculated by the following formula.

Formulation Judgment result (person) Stimulation rate (%) ++ + +- - Formulation Example 1 5 45 3.4 Formulation Example 2 One 4 45 4.0 Formulation Example 3 3 47 2.0 Comparative Example 1 2 48 1.3 Comparative Example 2 2 8 40 12.0 Comparative Example 3 4 46 2.6 Comparative Example 4 3 47 2.0 Comparative Example 5 3 4 43 6.6 Comparative Example 6 3 47 4.6 Comparative Example 7 4 46 2.0 Comparative Example 8 2 6 42 6.7 Comparative Example 9 3 47 2.0 -: No erythema or unusual phenomena +-: Slightly redder than the surroundings +: Slightly redder than the surroundings ++: Severe reddening and swelling than the surroundings

As shown in Table 5, the cosmetic composition of Formulation Examples 1-3 of the present invention shows almost similar skin irritation compared to the comparative example without sodium lactate, only the lactate is added and does not contain licorice extract Compared to Examples 2, 5 and 8, the degree of stimulation was very low. Therefore, it can be seen that the cosmetic composition of the present invention is less skin irritation and excellent skin safety, licorice extract reduces the skin irritation caused by lactate.

Experimental Example 2: Measurement of the whitening effect

The whitening effect of the cosmetic composition of the present invention was measured in 30 Korean female volunteers aged 19-40 with dark skin as follows. The measurement site was marked with nine sites of 1 × 1 cm 2 on the arm, and each sample (Formulation Examples 2 and 3 and Comparative Example 4-9) was continuously applied to eight sites, and one was treated as an untreated control value. Put it. Subsequently, the whitening degree (ΔL value) was measured at 2 weeks intervals for 2 months using the Minolta CR 300 measuring instrument. The results are shown in Table 6.

division Formulation Example 2 Formulation Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Control ΔL value 2.51 2.33 0.50 1.00 1.26 0.75 1.13 1.35 0

As shown in Table 6, the ΔL values of Examples 2 and 3 after 8 weeks were higher than those of Comparative Examples 4-9 and the control group. Therefore, it can be seen that the cosmetic composition of the present invention has an excellent whitening effect.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that the specific technology is merely a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

As described in detail above, the present invention provides a whitening cosmetic composition comprising lactate and licorice extract. The cosmetic composition of the present invention exhibits an improved whitening effect with very low skin irritation.

Claims (6)

A cosmetic composition for skin whitening comprising (a) 1-20% by weight lactate as a whitening agent and (b) 0.0001-15.0% by weight of root extract of licorice as an anti-irritant and whitening agent by the lactate. The cosmetic composition for skin whitening according to claim 1, wherein the content of lactate is 15% by weight. The cosmetic composition for skin whitening according to claim 1, wherein the content of the licorice extract is 10% by weight. The cosmetic composition for skin whitening according to any one of claims 1 to 3, wherein the lactate is 60% (w / v). The cosmetic composition for skin whitening according to any one of claims 1 to 3, wherein the lactate is sodium lactate. The cosmetic composition for skin whitening according to any one of claims 1 to 3, wherein the cosmetic composition for skin whitening is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation Cosmetic composition for skin whitening, characterized in that it has a formulation selected from the group consisting of emulsion foundation, wax foundation and spray.