KR101195576B1 - Cosmetic Composition Cyperus Papyrus Extracts for Skin whitenig - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening containing a papyrus extract, and more particularly, to contain a papyrus extract, which is a vegetable extract, to provide a skin whitening effect, and to regenerate skin cells by using a papyrus extract and sewage extract together. It is a skin whitening cosmetic composition that has excellent effects, inhibits melanin production, inhibits tyrosinase activity, and has anti-oxidant activity, which synergistically helps skin whitening. Papyrus and sewage are safe from skin irritation with natural ingredients. It is a substance.

Description

Cosmetic Whitening Cosmetic Composition Containing Papyrus Extracts {Cosmetic Composition Cyperus Papyrus Extracts for Skin whitenig}

The present invention relates to a cosmetic composition for skin whitening containing a papyrus extract, and more particularly, to contain a papyrus extract, which is a vegetable extract, to provide a skin whitening effect, and to regenerate skin cells by using a papyrus extract and sewage extract together. It is a skin whitening cosmetic composition that has excellent effects, inhibits melanin production, inhibits tyrosinase activity, and has anti-oxidant activity, which synergistically helps skin whitening. Papyrus and sewage are safe from skin irritation with natural ingredients. It is a substance.

Skin is a very important tissue that protects the human body while having direct contact with the external environment and has biochemical and physical functions. This tissue is largely divided into three parts: epidermis, dermis and subcutaneous tissue. Human skin color depends mainly on the number, size, type and distribution of melanocytes containing melanin in skin cells. Melanosomes are produced by melanocytes and melanin, a black pigment produced in the epidermis, is produced in cells called melanocytes. Melanin absorbs ultraviolet light energy from sunlight, preventing deeper cells from being damaged by ultraviolet light. When abnormally low melanin is produced, skin lesions such as vitiligo are induced, and on the contrary, excessive synthesis of melanin by ultraviolet light not only damages the skin, but also forms spots, freckles, and further causes skin cancer.

At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, whitening agents using tocopherols and vitamins used to reduce the melanin pigment are known to have a slight decolorizing effect of the melanin pigment. Therefore, attention is being paid to inhibitors that inhibit the production of melanin by inhibiting the activity of tyrosinase.

Conventionally, many studies on melanin production pathways have been conducted to prevent pigmentation, and raw materials for inhibiting melanin production by acting on each step thereof have been developed. For example, raw materials for inhibiting tyrosinase enzymes related to the synthesis of melanin pigment include arbutin and mulberry extract, and vitamin C derivatives that promote reduction of melanin produced. In addition, there are lactic acid and salicylic acid which promotes exfoliation of melanin in the epidermis to promote exfoliation of keratin, inorganic sunscreens and organic sunscreens that block ultraviolet rays, and hydroquinones that show cytotoxicity to melanocytes. have. Recent research on whitening has progressed to inhibit melanin production by blocking signaling substances such as cytokines that affect melanin synthesis.

However, the raw materials have a problem in cell penetration ability, the inhibitory effect on tyrosinase is insignificant, and some raw materials have the disadvantage of discoloring the skin. In addition, since the amount of use is limited due to safety, etc. when blending in cosmetics, the development of new whitening raw materials is continuously studied.

Overcoming the problems of the existing whitening agent as described above, while using a small amount than the existing whitening agent has an excellent whitening effect and it is necessary to develop a whitening agent without side effects such as irritation to the skin.

The present inventors earnestly researched to develop a cosmetic composition which is superior in whitening effect and excellent skin safety and excellent stability in the formulation, compared to the conventional cosmetics for whitening, the above objectives when mixing sewage extract in papyrus extract and papyrus extract It was confirmed that to achieve the present invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin whitening containing papyrus extract which is a vegetable extract.

Another object of the present invention is to provide a cosmetic composition for maximizing skin whitening effect and effectively improving skin tone by further comprising sewage extract in papyrus extract.

In order to solve the above problems, the present invention may include the following means.

Skin whitening cosmetic composition according to the present invention contains papyrus extract as an active ingredient.

Another skin whitening cosmetic composition according to the present invention is characterized in that it contains a papyrus extract and sewage extract as an active ingredient.

The papyrus extract and sewage extract are characterized by containing 0.001% to 10.0% by weight, respectively, in the cosmetic composition.

The composition is characterized in that it has a formulation selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder.

The present invention provides a skin whitening effect by containing a papyrus extract which is a vegetable extract.

In addition, the present invention by using the papyrus extract and sewage extract together, the skin cell regeneration effect is excellent, inhibits melanin production, inhibits tyrosinase activity, excellent antioxidant activity provides a synergistic help to skin whitening do.

The present invention relates to a cosmetic composition for skin whitening using papyrus extract or papyrus extract and sewage extract to help skin whitening by increasing antioxidant power and improving skin tone.

Hereinafter, the present invention will be described in detail.

Papyrus (Cyperus papyrus) is a plant belonging to the Cyperaceae, which has been cultivated for a long time in the Nile delta region of Egypt and gathered for writing sacks or stems. Make writing paper. Papyrus is a grass-like aquatic plant with dull triangular stems of woody nature, growing up to 4.6 meters in calm water, about 90 cm deep. Now it is often planted in ponds for ornamental purposes or in greenhouses in warmer areas. C.isocladus / C.papyrus 'nanus' grows up to 60 cm and is often planted in pots or raised in homes. Ancient Egyptians used papyrus stems to make sails, cloth, cushions, ropes and paper. The paper made was the main stationery of the ancient Egyptians, introduced by the Greeks and widely used in the Roman Empire.

Sewage ( Pleuropterus multiflorus ) used in the present invention is It is known to come from China and has been cultivated for a long time. The height is 3 ~ 4m and the roots are extended to the side, often forming tubers. Heart-shaped leaves are about 3-6cm long and 2.5 ~ 4.5cm wide, with petioles and chin leaves, but without jagged edges. Flowers bloom in August-September in conical inflorescences. There are no petals and 5 calyxes. Fruits are ovate, glossy, 2.5mm long.

The reddish brown tuber is called Shouo in one shot. Shouo is famous as a herb that strengthens kidney function, enhances energy, darkens hair and makes people live longer without disease. The function of the liver to get rid of tiredness, multiply the skin, strengthen the bones and muscles, and strengthen the heart is also effective in nervous breakdowns and insomnia. Its excellent hematopoietic effect makes it an excellent treatment for anemia and is widely used in women's menstrual disorders, uterus and chronic constipation. In addition, sewage is a good medicine to boost the energy of the elderly. Improves kidney function so that hair does not whiten and hair does not fall out. Chinese people consider HaoSuo to be the three greatest medicines with ginseng and wolfberry. Resveratrol, a substance found in sewage, is a substance belonging to the polyphenol family, which is an antioxidant found in plants, and is known to be contained in grapes, mulberries, and peanuts, and is particularly contained in red wine. Resveratrol has been known to have anti-cancer and anti-oxidant effects, and has also been shown to be effective in reducing coronary artery damage and preventing blood clotting, a dangerous phenomenon that causes heart attacks and strokes.

The present invention contains the papyrus and sewage extract as an active ingredient. The extract according to the present invention can be prepared as follows.

The papyrus and sewage are washed thoroughly with water and dried in the shade. Each dry plant is placed in an extraction container and extracted for a period of time at an appropriate temperature in an appropriate amount of extraction solvent. The extract may be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as hot water extraction, reflux cooling extraction, ultrasonic extraction, etc., and may be extracted once to five times, preferably as an extraction method of hot water extraction.

The immersion temperature is preferably 30 to 70 ° C, more preferably 40 to 60 ° C, and most preferably 50 ° C or so, which is most effective in preventing decay, discoloration and detachment.

The immersion time is 5 to 20 hours, preferably 10 hours.

After the extract is obtained, solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

 The extract of the present invention preferably comprises 0.001 to 10.0% by weight relative to the total cosmetic composition. When the extract of the present invention contains less than 0.001% by weight, the skin moisturizing effect of the cosmetic composition is insignificant, and when added in excess of 10.0% by weight, the synergistic effect due to the overdosing is not economical, and the product stability is not good. Can affect More preferably, the extract of the present invention preferably contains 0.1 to 5.0% by weight, respectively.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to papyrus extract and sewage extract as active ingredients, and include, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, pigments and perfumes, And carriers.

The cosmetic composition for skin whitening of the present invention may contain other ingredients, such as papyrus extract and sewage extract, within the range of not impairing the desired effect of the present invention, preferably increasing the whitening effect. In addition, it may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powders It may be formulated as a foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, the carrier components include animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide. Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminium hydroxide, calcium silicate or polyamide powder may be used, especially in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Aliphatic esters of 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethiotate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide. Ethersulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanol amides, vegetable oil lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following Examples and Experimental Examples are only for more specifically describing the present invention, and the scope of the present invention is not limited by them according to the gist of the present invention. It will be obvious to him.

Preparation Example 1 Preparation of Papyrus Extract

50 g of papyrus was immersed in 200 ml of 100% ethanol and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered with filter paper to obtain a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Preparation Example 2 Preparation of Sewage Extract

50 g of sewage was taken and immersed in 200 ml of 100% ethanol and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered with filter paper to obtain a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experimental Example 1 Intracellular Melanin Inhibitory Effect

B16F1 melanoma cells were provided by the Korea Cell Line Bank and cultured. B16F1 melanoma cells are cell lines producing melanin and are generally used for whitening screening.

The medium for culturing the cells was sterile by adding DMEM, 10% FBS, 1% penicillin / streptomycin. Cell cultures were incubated in an incubator at 37 ° C., 5% CO ₂ . Melanoma cells were cultured and passaged when the 100 mm Petri dishes were about 90% full.

To measure the whitening effect of papyrus extract and sewage extract, the amount of melanin produced by treatment with B16F1 melanoma cells was measured. Cells were seeded on 6-well plates, the papyrus extract prepared in Preparation Example 1 and the sewage extract prepared in Preparation Example 2 were treated at 200 µg / ml, and then cultured for two days to recover the cells. The recovered cells were added with 1N NaOH containing 10% DMSO, reacted at 90 ° C for 1 hour to be sufficiently dissolved in melanin, and then absorbance was measured at a wavelength of 405 nm using an ELISA reader.

Name of sample Melanin production (%) Untreated 100 Arbutin 75.3 Papyrus extract 73.8 Sewao Extract 50.6

Experimental results were calculated using the equation (1) to calculate the inhibition, shown in Table 1. Arbutin, which is known as a general whitening substance, has a melanin content of 55.3%, which is about 1.5 times higher than that of arbutin, while Arbutin has a melanin content of 75.3% at a concentration of 200 ㎍ / ml. Papyrus extract has also been found to have a skin whitening effect similar to arbutin.

[ Experimental Example  2] Determination of Tyrosinase Activity Inhibition

It was determined whether the extract affects tyrosinase enzyme activity, which plays an important role in producing melanin.

210 μl of 0.1 M phosphate buffer (pH6.8) is added to the 96 well plate, and 20 μl of mushroom-derived tyrosinase is added after adding 30 μl of sewage extract or distilled water with a final concentration of 200 μg / ml. After adding 40ul of 5mM L-DOPA as a substrate and reacting for 10 minutes at 37 ℃, the absorbance was measured at a wavelength of 475nm using an ELISA reader.

Tyrosinase activity was calculated using Equation 2 below. Table 2 shows the melanin inhibitory effects of kojic acid and the extracts, which were widely used as whitening agents.

Name of sample Tyrosinase activity (%) Untreated 100 Kojisan 3.6 Papyrus extract 60.5 Sewao Extract 45.3

In treating koji acid, only 3.6% of the total activity was active, whereas sewage extract showed 45.4% of activity. Although the inhibitory effect was less than that of kojic acid, papyrus extract and sewage extract also showed better inhibitory effect on tyrosinase enzyme than untreated group.

[ Experimental Example  3] toxicity test

In order to use the extract as a whitening cosmetic, an experiment was performed to determine whether or not cytotoxicity. Cells were inoculated into 96 well plates, treated with papyrus extract and 200 g / ml of sewage extract. After 2 days, the medium was removed, 100 μl of MTT solution was added, and the solution was removed at 30'C for 4 hours, followed by staining with 100ul of DMSO. The cells were thawed and the absorbance was measured at a wavelength of 540 nm using an ELISA reader.

Cell viability was calculated using the following Equation 3, the results are shown in Table 3.

Name of sample Cell survival rate (%) Untreated 100 Arbutin 97.8 Papyrus extract 98.6 Sewao Extract 98.7

Compared with the cells not treated with arbutin, the papyrus extract and sewage extract have a high survival cell rate despite high concentration of 200 μg / ml, indicating little cytotoxicity.

[ Experimental Example  4] Antioxidant Activity Test

In order to determine whether the papyrus extract and sewage extracts have antioxidant activity, it was generally measured using a DPPH solution, which is a method of measuring antioxidant activity.

200 μg / ml of the extract and 100 μl of DPPH solution, which is an antioxidant measurement solution, were mixed and reacted at 37 ° C. for 30 minutes, and then absorbance was measured at a wavelength of 517 nm using an ELISA reader.

Experiments with vitamin C, known as antioxidants, compared the relative activity of extracts.

Name of sample Vitamin C Papyrus extract Sewage  extract Antioxidant activity (%) 70.4% 50.8% 89.9%

As a result, as shown in Table 4, the sewage extract has a strong antioxidant activity of 89.9%, and papyrus extract seems to have excellent antioxidant activity. Moreover, sewage extract shows better antioxidant activity than vitamin C.

Examples and comparative examples for the compositions of the present invention are illustrated below.

[Production Example 3]

To prepare a cosmetic composition containing papyrus extract, sewage extract in the form of an emulsion having a composition ratio of Table 5.

ingredient Example 1 (% by weight) Example 2 (% by weight) Comparative Example 1 (% by weight) Comparative Example 2 (wt%) Papyrus Extract 2.5 5.0 Sewao Extract 2.5 5.0 glycerin 5.0 5.0 5.0 5.0 Liquid paraffin 8.0 8.0 8.0 8.0 Polysorbate 60 2.0 2.0 2.0 2.0 Sorbitan sesquirololeate 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 Wax 2.0 2.0 2.0 2.0 Triethanolamine 0.5 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 Purified water Balance Balance Balance Balance system 100 100 100 100

[ Experimental Example  5] whitening effect

In order to measure the whitening effect of the present invention, 40 healthy women in their 30s and 40s for 1 month were divided into four groups of 10 and 1 sample each of Examples 1 and 2 for twice a month. The black and white degree of skin was apply | coated and the effect was judged using the color difference meter (Minolta CR2002). L *, a *, b * color system is used to display color. In this experiment, L * value (brightness) was mainly used as an index. The L * values range from black (L * = 0) to white (L * = 100) and ΔL * after one month is shown in Table 6 below.

Test substance ΔL * Example 1 10.6 Example 2 3.62 Comparative Example 1 5.98 Comparative Example 2 0.41

As can be seen from the above [Table 6], the papyrus extract and sewage extract in the embodiment containing all of the compositions of the present invention was found to exhibit the best whitening effect, even in Example 2 used alone papyrus extract It was confirmed that the excellent ㅁ whitening effect.

[ Experimental Example  5] Skin safety test

In order to confirm the skin safety of the present invention for 20 women in their 20s and 30s who have never had a hypersensitivity reaction to skin irritation in the past and have no skin disease or skin allergy symptoms, The skin patch test was done. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. The skin reaction was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the average skin response rate was calculated according to Equation 5 below. The results are shown in Table 8 below.

[Evaluation Criteria and Score of Skin Response] Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe erythema and alveolar, scleroderma

Test substance After 1 hour After 24 hours Reactivity (%)
(n = 20) ± + ++ ± + ++ Example - - - - - - 0.00 Comparative Example 1 - - - - - - 0.00 Comparative Example 2 - - - - - - 0.00 Comparative Example 3 - - - - - - 0.00

As can be seen from the results of Table 8, the papyrus extract and sewage extract in the skin patch experiment can be seen that the material is very excellent safety without skin irritation.

Hereinafter, as an example of the cosmetic formulation of the present invention, a flexible lotion, a nourishing lotion, a nourishing cream, and a massage cream containing the papyrus extract and a sewage extract of the present invention are exemplified, but the formulation of the cosmetic of the present invention is not necessarily limited thereto. .

Formulation Example 1 Flexible Cosmetics

Softening cream containing papyrus extract was prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus Extract 2.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

[Formulation Example 2] Lotion

Lotions containing papyrus extract and sewage extract were prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus Extract 2.5 Sewao Extract 2.5 Cetostearyl alcohol 1.0 Glyceryl monostearate 0.8 Sorbitan monostearate 0.3 Porofilparaben 0.1 Polysorbate 60 1.0 Mineral oil 5.0 Pyromethicone 3.0 Dimethicone 0.5 Allantoin 0.1 glycerin 5.0 Alcohol 2 Propylene glycol 3.0 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 3 Essence

An essence containing papyrus extract and sewage extract was prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus Extract 2.5 Sewao Extract 2.5 1,3-butylene glycol 4.0 glycerin 3.0 Allantoin 0.5 Panthenol 0.1 EDTA-2Na 0.01 ethanol 5.0 Triethanolamine 1.5 Squalane 2.0 Wax 2.5 Polysorbate 60 3.5 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 5 Massage Cream

Massage cream containing papyrus extract and sewage extract was prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus Extract 2.5 Sewao Extract 2.5 glycerin 4.0 Vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 6 Shampoo

Shampoo containing papyrus extract and sewage extract was prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus Extract 2.5 Sewao Extract 2.5 Alkyl ether sulfate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 3.0 Lauramid 4.0 EDTA-2Na 0.1 Polyquaternium-10 0.05 NaCl 0.1 Citric acid 2.4 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 7 Body Cleanser

A body cleanser containing papyrus extract and sewage extract was prepared according to a conventional method.

Raw material name Content (% by weight) Papyrus extract 2.5 Sewao Extract 2.5 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Balance system 100

Claims (4)

delete Cosmetic composition for skin whitening containing papyrus extract and sewage extract as an active ingredient. The cosmetic composition for skin whitening according to claim 2, wherein the papyrus extract and the sewage extract each contain 0.001% to 10.0% by weight in the cosmetic composition. The composition of claim 2 or 3, wherein the composition is selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Cosmetic composition for skin whitening, characterized in that it has a formulation.