KR101028701B1 - Cosmetic compostions comprising Camellia japonica stamen with pollen and its extract for improving skin - Google Patents

Abstract

The present invention relates to the use of camellia surgery or its extracts in cosmetics, and more specifically, by containing a camellia powder or camellia extract extract, it is possible to supplement and improve skin with excellent moisturizing power as well as removing skin waste and old dead skin cells. The present invention relates to providing an excellent cosmetic that can impart a whitening effect on the skin.

Camellia Surgery, Camellia Surgery Extract, Moisturizing, Whitening

Description

Cosmetic composition for improving skin whitening including camellia surgical powder or camellia surgical extract {Cosmetic compostions comprising Camellia japonica stamen with pollen and its extract for improving skin}

The present invention relates to the use of camellia surgery or its extract in cosmetics, more specifically, by containing a large amount of dried camellia powder or camellia extract extract in the cosmetics, as well as excellent removal of waste and dead skin due to skin friction It relates to a cosmetic composition that imparts a moisturizing and whitening effect to the skin.

As the awareness of a healthy and beautiful body is growing, regardless of age and gender, specialty beauty salons are booming as well as increasing interest in self-care. For this reason, natural scrubs that are commonly available in the surroundings are in the spotlight.

Typically, the scrub particles contained in the cosmetics are mechanically pulverized salt, sugar, barley, green tea, dried apricot seeds, peach seeds, walnut shells, etc. to use the particles or spherical polyethylene powder and synthetic polymer particles.

However, in the case of natural particles, it may cause skin troubles such as minute wounds due to uneven appearance of the particles, and synthetic polymer particles are not easily crushed when rubbed on the skin, resulting in inconvenience in use. In addition, the synthesized polymers have a disadvantage that they do not impart any physiological biochemical effects on the skin other than the scrub function. For this reason, natural scrubs are widely used due to the advantage that some active ingredients are applied to the skin during scrubs despite various inconveniences. Therefore, natural scrub can minimize skin irritation and maximize skin improvement, which can be a very important material commercially.

Camellia is an evergreen tree belonging to theaceae family, and its origin is reported to be about 200 species in Asia, and one of them is Camellia in Korea. japonica L ..) is spontaneous and is called camellia.

Camellia has been used mainly as a horticultural resource. Since ancient times, wood has been used as a craft material, stalks as a raw material of high-grade charcoal, and seeds have been used for a limited amount of oil such as cooking oil, cosmetic oil (head oil), and orange oil. I used to make hwajeon to eat.

Existing patents related to camellia are also registered for the use of camellia oil, camellia flowers, camellia seeds, and camellia leaves, as in the case of Cosmax (Korean Patent No. 10-0556524) and Bioland (Korean Patent No. 10-0708236). Or, it has been filed, but the scope of use and use of other parts of the camellia is insufficient. Thus, the scope and value of the study have been studied in more detail.

Camellia flowers bloom from November to March of the following year, and the flowers are peculiar to fall after fertilization. In particular, even after a few minutes, the pot and the surgical bag that keeps the pot are still attached to the flower, and fall off when they fall on the petals. Therefore, camellia flowers can be said to be easier to harvest by separating the stamens including pollen than any other flowers. Since camellia surgery is easy to harvest, it can be used as a scrub or soap material after collecting and drying the surgery. In the structure of flowers, surgery is a tissue for fertilization of plants, so various plant hormones, vitamins, and various minerals are more abundant than any tissue. Some plants may store starch, sugar, or fat, and some animals may use pollen as food. In addition to nutrients, many enzymes are often contained, so some organisms use these enzymes. In some species, pollen loses its function in a few minutes after leaving the anther, and some have a lifespan of over one year, like date palms. In general, it has a lifespan of 2 to 3 days, but it can be stored longer in a dry state, and because of its rigid structure, it also remains a plant fossil. For this reason, that is, considering the rigid structure and the high concentration of various nutrients present, it can be said that it is easy to use as a scrub particle and can be used for skin improvement.

The present invention relates to the invention that the camellia surgery powder obtained by drying and grinding the camellia surgery or the camellia surgery extract obtained by extracting the camellia surgery can impart an improvement effect on the skin.

The research team tried to find ways to remove wastes and exfoliate skin, and to feel good, to moisturize the skin, and to improve the skin through whitening effect.In conclusion, camellia powder or camellia extract is especially useful for skin improvement. The present invention was completed by finding out that the whitening effect is excellent and confirming that it can be used for new whitening purposes.

Therefore, the camellia surgical powder or camellia surgical extract obtained in the present invention may be prepared into various kinds of cosmetic compositions containing suitable additives according to the use purpose and method.

The composition containing the camellia surgery powder or camellia surgery extract according to the present invention is excellent in skin moisturizing effect, high safety can be used as a composition for skin moisturizing. In addition, the Camellia Surgery Powder and Camellia Surgery Extract are excellent in whitening effect and can be applied to functional cosmetics to give whitening effect.

An object of the present invention relates to a cosmetic composition containing a camellia surgery powder or a camellia surgery extract, in the case of camellia surgery is not irritated to the skin as much as possible and excellent in use as well as the scrub particles themselves to moisturize the skin It is used for skin improvement through whitening effect. In addition, the extract is used for skin improvement by extracting to use the active ingredient contained in the surgery more directly. In particular, the camellia surgery extract has been found in the present invention that has an excellent whitening effect that does not appear in other camellia areas can be used to give skin improvement, especially whitening effect.

In order to achieve the object of the present invention, the present invention is dried in a dryer or a freeze dryer maintained at a high temperature after separating the operation by collecting the flowers at the time of flowering or by dropping the flowers after fertilization. Surgery obtained by this process is used directly or extracted by a known method to be used as a surgical extract.

In the present invention, the camellia surgery extraction method can be extracted by a variety of known extraction methods. For example, one obtained by a hot water extraction method, one obtained by solvent extraction, one obtained by supercritical extraction, or by ultrasonic waves.

Since the present invention explores the potential as a material to be used in cosmetics, the following invention was advanced using 70% ethanol extract powder.

Although the content of this invention is demonstrated to the following implementation, the content of this invention is not limited to this.

Example 1 Preparation of Camellia Surgery

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In February and February, after flowering of Camellia japonica L. in Jeju Island, fallen flowers are collected and refrigerated (4 ℃). At this time, the detached operation with the camellia petals separated. The isolated surgery is dried in a 50 ° C. dryer. Drying process is possible from 30 ℃ to 70 ℃. The dried procedure is crushed completely with a propeller rotating at 3000 rpm to 10000 rpm for about 1 to 10 minutes using a commonly used brander or mixer. After grinding, the mixture was sieved through a 50 mesh sieve to obtain dry camellia powder with a particle size of 10-200 μm. The camellia powder thus obtained may be used as it is in formulations such as scrubs and soaps. However, products such as skins or lotions, which should not contain suspended solids, are used in the production of extracts using various extraction methods.

Example 2 Preparation of Camellia Surgery Hydrothermal Extract

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5 g of dried camellia powder was added to 100 g of water and boiled at 100 ° C. for 10 minutes to obtain a hot water extract. The hot water extraction method used in the present invention can also be obtained by 121 ° C. autoclave method or other similar various methods.

Example 3 Preparation of Camellia Surgical Organic Solvent Extract

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5 g of dried camellia surgical powder particles were immersed with ethyl acetate, methyl alcohol (80%) or ethyl alcohol (70%) in an organic solvent for 3 days, and then filtered with a 1 micrometer filter paper to obtain a clean extract. The obtained primary extract was concentrated by a general concentration method to prepare a dry powder. When 500g of solvent was used for the integrated 10g of dry powder, about 2g of dry extract was obtained. In the case of the organic solvent extraction method, it is possible to increase the extraction efficiency by adding an ultrasonic method.

Example 4 Preparation of Camellia Surgery Supercritical Extract

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5 g of dried camellia powder was prepared by supercritical extraction using carbon dioxide.

Example 5 Inhibitory Effects of Extracts on Melanin Production

Rat B16 melanoma cells (Korea Cell Line Bank) were used to measure the inhibitory effect of melanin production by alpha bisabollol and compared with the melanin inhibitory effect by arbutin, which is known to inhibit melanin production.

In this experiment, murine melanoma (B-16 F10) cells (Korea Cell Line Bank) were used in DMEM medium containing 10% fetal bovine serum (FBS) in a 6-well plate at 1 × 10 ⁵ per well. After inoculation with dogs, the cells were incubated at 5% CO ₂ and 37 ° C until at least about 80% of the cells adhered to the bottom of the well. After incubation, the medium was removed and the sample was replaced with medium diluted to an appropriate concentration and incubated at 5% CO ₂ , 37 ° C for 3 days. Treatment concentration of the extract was determined to be 0.01%, 0.1% without cytotoxicity. The cells from which the medium was removed were washed with PBS (phosphate buffer saline) and treated with trypsin to recover the cells. The recovered cells were measured by using a hematocytometer (Tiefe Depth Profondeur 0.100 mm, Paul Marienfeld GmbH & Co. KG, Germany), and then centrifuged at 5,000 to 10,000 rpm for 10 minutes. It was removed to obtain pellets. The cell pellet was dried at 60 ° C., and then 100 μl of 1 M sodium hydroxide solution containing 10% DMSO was added to obtain intracellular melanin in a 60 ° C. thermostat. Using this solution, the absorbance was measured at 490 nm using a microplate reader (Bio-Tek ELx8081U, USA) to determine the amount of melanin per cell. The results are shown in Table 1 below.

Melanin production inhibitory effect Kinds Hot Water Extract Methyl Alcohol (80%) Extract Ethyl Alcohol (70%) Extract Supercritical extract Positive control group 0.01% 0.1% 0.01% 0.1% 0.01% 0.1% 0.01% 0.1% Arbutin (5 ppm) Melanin production inhibition rate (%) 19 39 25 47 26 49 32 65 22

As shown in Table 1, it was found that both camellia surgery extract inhibits melanin production. Among the various extraction methods, the extract prepared by supercritical extraction showed the highest melanin production inhibition rate.

Example 6. Search for whitening efficacy by site

In order to confirm that the whitening effect shown in Example 5 is also shown in other parts of the camellia, flower extracts (whole flowers), stem extracts, petal extracts, leaf extracts, seed extracts, and root extracts were prepared by using a supercritical extraction method. The whitening efficacy of each of these extracts was assayed in the same manner as in Example 5. The results are shown in Table 2 below.

Melanin production inhibition rate Kinds Flower
extract stem
extract petal
extract leaf
extract strain
extract Root
extract Camellia Surgery
extract Positive control group 0.1% 0.1% 0.1% 0.1% 0.1% 0.1% 0.1% Arbutin (5 ppm) Melanin production inhibition rate (%) - - - - - - 66 25

As shown in Table 2 above, no whitening effect was observed at other parts of the camellia. In particular, the flower extract did not appear whitening effect even though it contains stamens, which is not shown at the same concentration because the ratio of stamens is small in the composition of the flower. Another possibility is that the parts constituting flowers other than stamens contain substances that counteract the effect of the whitening effect of the stamens. Therefore, the whitening efficacy not reported in camellia appears only in surgery can be said to be included in the new invention.

Example 7 Moisturizing Effect

The degree of transepidermal water loss (TEWL) was measured using a TEWA (Tewameter TM210, Germany) evaporator. The measurements were made by treating the cream of Camellia Surgery Extract (0.1%) prepared above and the cream containing no Camellia Surgery Extract each, and performing it once every 4 weeks and then comparing the results. All measurements were taken in a constant temperature and humidity room with no air movement, no direct sunlight, and a room temperature of 22 ° C and a relative humidity of 40%. In 30 subjects, the measurement site was the abdomen. As shown in Table 3 below, in the case of the nutrient cream containing camellia surgical extract, all the water loss was reduced.

실시예 8. 피부개선 화장료 조성물의 제조

Example 8. Preparation of skin improvement cosmetic composition

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1) Scrub Massage Cream

As follows, a scrub massage cream containing the camellia surgical powder obtained in Example 1 was prepared in a conventional manner.

Example preparation of the scrub and massage cream containing the extract of Example 1 is as shown in Table 4. It dissolved by heating at 70 degreeC purified water, triethanolamine, and butylene glycol. The oil phase component, emulsifier, and preservative which melt | dissolved at 70 degreeC were added and emulsified here. After the emulsification was completed, the solution was cooled to 45 ° C., and then pigments and flavors were added and dispersed, followed by cooling to 30 ° C.

Scrub and Massage Cream Containing Camellia Surgical Powder ingredient Content (% by weight) Camellia Surgery Powder 1.0 Liquid paraffin 40.0 Pluylene glycol 2.0 Squalane 5.0 Sorbitan sesquioleate 0.8 Polysorbate 60 1.5 Capric & Capric Triglycerides 4.0 Concentrated glycerin 6.0 Butylene glycol 4.0 beeswax 10.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Remainder

2) pack

As follows, packs containing the camellia surgical extract obtained in Examples 2, 3 and 4 were prepared according to a conventional method.

Examples of preparation of scrubs and massage creams containing the extracts of Examples 2, 3 and 4 are as shown in Table 5 below. Purified water, triethanolamine, and the like, which were aqueous, were dissolved by heating at 70 ° C. The oil phase component, emulsifier, and preservative which melt | dissolved at 70 degreeC were added and emulsified here. After the emulsification was completed, the solution was cooled to 45 ° C., and then pigments and flavors were added and dispersed, followed by cooling to 30 ° C.

ingredient Content (% by weight) Camellia Surgery Extract 1.0 ethanol 5.0 Allantoin 0.2 Triethanolamine 0.1 Sodium Carboxymethyl Cellulose 0.2 Polyvinyl alcohol 13.0 antiseptic a very small amount Fiji 60 / Hydrogenated / Caster Oil 0.2% Spices a very small amount Purified water Remainder

3) lotion

A preparation example of a lotion containing a camellia surgical extract is shown in Table 6 below. The aqueous phase purified water, triethanol amine and butylene glycol were dissolved by heating to 70 占 폚, and the oily fatty acid, oily component, emulsifier, and preservative were heated to 70 占 폚 to dissolve the solution. After the emulsification was completed, a 2% solution of xanthan gum, a hydrophilic thickener, was added to adjust the amount to 0.05% by weight. After cooling the solution to 45 ℃, camellia surgical extract, flavor and pigment was added and mixed and then cooled to 30 ℃.

Components and Contents of Lotion Containing Camellia Surgery Extract ingredient Content (% by weight) Camellia Surgery Extract 1.00 glycerin 3.00 Carbomer 0.10 Xanthan Gum 0.05 1,3 Butylene Glycol 3.00 Polyglycerin-3 Methylglucose Distearate 1.50 Glycerin Stearate 0.50 Cetearyl Alcohol 0.30 Jojoba oil 3.00 Floating paraffin 2.00 Squalane 3.00 Dimethicone 0.50 Tocopheryl acetate 0.20 Triethanolamine 0.10 Preservative, fragrance, coloring a very small amount Purified water Balance Sum 100

4) cream

A preparation example of the nourishing cream containing the camellia surgical extract is shown in Table 7 below. The aqueous phase purified water, triethanol amine, and propylene glycol were heated and dissolved at 70 ° C, and the oily fatty acid, oil component, emulsifier, and preservative were heated to 70 ° C to dissolve, and then emulsified. After emulsification was completed, the solution was cooled to 45 ° C, camellia surgical extract and flavor was added and dispersed, and then cooled to 30 ° C.

Ingredients and Contents of Nutrition Cream Containing Camellia Surgery Extract ingredient Content (% by weight) Camellia Surgery Extract One% Jojoba oil 5.0 Floating paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 Methyl Glucose Disteare 2.0 Glyceryl stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxy Vinyl Polymer 0.3 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Balance Sum 100

Example 9 Evaluation of Whitening Effect at Animal Level

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Similar to humans, the whitening effect of alpha bisabolol was measured using brown malmoto (Tortoiseshell guinea pigs; Brown River Guinea pigs, Charles River Laboratories, Inc.), which is known to cause pigmentation by ultraviolet rays.

In order to cause pigmentation due to ultraviolet (UV) in the brown molemoto, a light shielding aluminum foil having a square window of 3 × 3 cm ² is adhered to the skin from which the hair of the brown molemoto coordination is removed, and then SE lamp ( Ultraviolet rays were irradiated with a wavelength of 290-320 nm (Toshiba) (total amount of irradiation energy = 1350 mJ / cm ² ). After UV irradiation, the aluminum foil was peeled off and a sample (camellia surgery extract and arbutin) was applied in the following manner. Pigmentation appeared 2-3 days after UV irradiation and reached a peak after about 2 weeks, from which each sample was applied.

The number of coatings was continued once or twice a day for 50 days. The sample was dissolved and diluted using a specific solvent (propylene solvent: ethanol: water = 5: 3: 2: mixed solvent), and applied with a cotton swab, and prepared a control site for necessarily applying the solvent to other sites. Along with the determination of effects, the cumulative stimulus was also observed.

The effect was determined by measuring the black and white degree of the skin using a color difference meter (CR2002 chromameter, Minolta, Japan), the results are shown in Table 8 below. L ^* A ^* B ^* color system is used to display color, and L ^* value was used as an index in this invention. L ^* value was calibrated with a standard whiteboard, and L ^* value was measured 5 times or more at one site, and pigmentation was equalized. The difference (ΔL ^* ) of the skin color at the start of application and at the time of completion was determined according to the following equation (1), and the effect was determined by this value. The results are shown in Table 8 below.

△ L ^* = L ^* value after 00 days application - L ^* value of the coating start date

Comparing the ΔL ^* value with respect to the sample application site and the control site, the effect of the whitening material can be seen.

sample Treatment Concentration (%) Whitening effect (△ L) Camellia Surgery Extract 0.1 0.45 Camellia Surgery Extract 1.0 0.68 Arbutin 1.0 0.59 Control - 0.35

As shown in Table 8, the camellia surgical extract showed a whitening effect concentration-dependently. In addition, camellia surgical extract showed better whitening effect than arbutin, and cumulative irritation was not observed during application test of the test substance.

Example 10 Skin Accumulation Irritation Test

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Using a cream manufacturing method in Example 8-4) to prepare a cream containing 5%, 10% camellia surgical extract. Using the prepared creams, a total of nine 24-hour cumulative patches on the upper forearm were performed every 30 days in 30 healthy adults to determine whether the camellia extract was irritating to the skin.

The patch method used the fin chamber (Finn chamber, Epitest Ltd, Finland). 15 µl of each of the external skin preparations was added dropwise to the chamber, followed by patching. The degree of response to the skin each time was scored using Equation 1 below, and the results are shown in Table 9 below.

Average responsiveness = [{(response x responsiveness) / (total number of subjects x highest score (4 points))} x 100] ÷ number of tests (9 times)

At this time, ± 1 point, + 2 points, + + 4 points in the reaction degree, and the average response was determined to be a safe composition when less than 3.

Test substance Number of subjects with response Average reactivity 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + Control 2 - - --- --- --- --- --- --- --- --- 0.18 Test group 1 (5% camellia extract extract cream) 3-- One - - --- --- --- --- --- --- --- 0.37 Test group 2 (10% cream containing camellia extract) 3-- One - - --- --- --- --- --- --- --- 0.37 Inspector 30 30 30 30 30 30 30 30 30

In the test groups 1 and 2 in Table 9, the number of people corresponding to ±, +, and ++ was 3, 0, and 1, respectively, and the rest did not appear. When calculated according to the formula described above, the average reactivity was 0.37, which was 3 or less, which was determined to be a safe composition.

As shown in Table 9, the cream containing the camellia surgical extract (test groups 1 & 2) did not show a distinct cumulative stimulation pattern as a control and was determined to be a safe substance for human skin.

Claims (6)

Cosmetic composition for improving skin whitening comprising camellia surgery powder or camellia surgery extract. The cosmetic composition for improving skin whitening of claim 1, wherein the camellia surgery is a state obtained from a camellia flower, dried camellia surgery, or frozen camellia surgery. The cosmetic composition for improving skin whitening according to claim 1, wherein the camellia surgical extract is obtained by a hot water extraction method, a solvent extraction method, a supercritical extraction method, or an ultrasonic extraction method. The composition of claim 1, wherein the composition has one formulation selected from the group consisting of skin, lotion, cream, essence, serum, foundation, pack, soap, cleanser, shampoo, rinse, hair tonic, patch, and scrub. Cosmetic composition for improving skin whitening, characterized in that. According to claim 1, wherein the camellia surgery powder is a cosmetic composition for improving skin whitening, characterized in that contained in an amount of 0.01 to 20% by weight based on the total weight of the composition. According to claim 1, wherein the camellia extract extract is a cosmetic composition for improving skin whitening, characterized in that contained in an amount of 0.01 to 40% by weight based on the total weight of the composition.