KR102208482B1 - Cosmetic composition for improving skin troubles - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin troubles. More specifically, the present invention relates to a cosmetic composition comprising a composite extract composed of a snapdragon extract, a Hemistepa lyrata extract, and a water pepper extract, sponge spicules powder, and edible sulfur at a particular ratio, and having excellent effects of moisturizing skin, soothing skin, and alleviating skin inflammation and skin troubles such as acne. The composition according to the present invention can be safely used without a side effect to skin, and has excellent anti-inflammation, skin regeneration, and wound healing improving effects. Thus, a cosmetic composition for moisturizing skin, soothing skin, and alleviating skin inflammation and skin troubles such as acne can be usefully used.

Description

Cosmetic composition for improving skin troubles

The present invention relates to a cosmetic composition for improving skin troubles, and more particularly, a complex extract consisting of a snapdragon extract, a gorilla extract, and a yellowtail extract, sponge powder and dietary sulfur in a specific ratio to moisturize the skin, sooth the skin, and skin inflammation And it relates to a cosmetic composition having an excellent effect of improving skin troubles such as acne.

The inflammatory reaction that appears on the skin is represented by five phenomena such as redness, tingling, burning, swelling, and tissue change.This is a physiological reaction to protect the living body from the invasion of foreign substances such as bacteria and mechanical damage. to be.

Representative types of skin troubles related to inflammation include atopic dermatitis and acne.In particular, the cause of atopic dermatitis has not been sufficiently identified, but immune hypersensitivity reactions or T lymphocytes following an increase in IgE, an immunoglobulin associated with allergies. It is known to be due to lack of function.

On the other hand, in oriental medicine, it is believed that the heat poison hidden deep in the body rises to the skin layer and causes various troubles for the cause of atopic dermatitis. In addition, since heat poisoning not only causes skin abnormalities, but also causes various complications by lowering the body's immunity, it is reported that a fundamental treatment is needed to find the cause of atopic dermatitis rather than simply treat symptoms. In other words, in Oriental medicine, it is said that the recurrence can be prevented only by releasing heat poisoning, which is the cause of atopic dermatitis, and increasing autogenous power to fight allergies.

Among the causes of atopic dermatitis that have been discussed in various ways, the abnormal body antioxidant system and the generation of excessive reactive free radicals are recently considered important. Originally, free radicals act as a defense mechanism to destroy exogenous pathogens that have penetrated into the body as a part of the immune system, but the sensitivity of occurrence in the body and skin of atopic dermatitis patients is greatly increased, so excessive free radicals rather damage the skin barrier or skin cells. It is known to damage.

The underlying cause of the immune hypersensitivity reaction and the excessive generation of free radicals is not clear, but the function of the immune system is caused by internal and extrinsic stress caused by the influx of environmental hormones such as dioxin into the human body in addition to genetic causes. It is known to be caused by deterioration and destruction. In addition, damage to the skin barrier accompanying atopic dermatitis, secondary bacterial infection, increased susceptibility to drug side effects, and decreased resistance to infections such as fungi and viruses also appear.

As a method of treating such atopic dermatitis, methods of removing allergens or improving the immune system are being considered.Since it is difficult to remove allergens, treatments related to immunity and drugs to lower histamine secretion from mast cells are considered. It is a common way to receive.

Currently used treatment methods include using immunosuppressants to alleviate various symptoms caused by atopic dermatitis or to apply a moisturizer to prevent skin dryness in atopic patients and to treat the accompanying inflammation. Steroids such as the following are used. However, long-term use of these steroids has a disadvantage in that it causes various skin side effects such as steroidal acne, skin atrophy, and capillary vasodilation. In addition, antihistamines are used as a temporary measure to alleviate itchy symptoms by preventing histamine from being released from mast cells. Long-term use may have side effects such as insomnia, anxiety, and loss of appetite. Therefore, there is a need to develop a material that does not exhibit such side effects and exhibits an excellent anti-inflammatory effect.

On the other hand, in recent years, laser treatment or dermabrasion is popular for the purpose of beauty or disease treatment. Laser treatment is the treatment of single wavelength light using the characteristics of light radiation, transmission, absorption, and energy transfer. In the sense of a single wavelength, therapeutic lasers vary according to wavelength and energy emission method. In other words, treatment lasers are different for each disease. When the laser beam hits the body, it turns into heat, and the temperature of the area absorbed by heat rises. When it exceeds 40 degrees, protein degeneration begins. If it is more than 68 degrees, blood coagulation is more than 100 degrees, carbonization or vaporization. On the other hand, below 40 degrees, the cell tissue is activated because blood circulation and metabolism improve and increase. Treatment using photostimulation of 40 degrees or less is called low-response laser treatment, and thermal stimulation of 40 degrees or more is called high-response laser treatment.

Peeling is a procedure to induce the generation of new epidermis and stimulate the production of collagen fibers in the dermis by peeling off a part of the skin to varying degrees according to the type of dermabrasion using chemicals or lasers. It is used to treat acne, scars, wrinkles, pigmented lesions, and enlarged pores. Classification according to the peeling method includes chemical dermabrasion, which uses chemicals to peel the skin, and laser dermabrasion, which uses a laser to peel the skin.The classification according to the depth of the skinning is superficial dermabrasion, which only peels off the stratum corneum or the epidermis, and the upper dermis layer. There is a middle layer dermabrasion that peels up to and a full layer dermabrasion that peels off the lower part of the dermis.

After such laser treatment or dermabrasion, it is important to induce skin regeneration without inflammatory reaction.To this end, cosmetics for skin regeneration or wound healing have been developed in various ways, but most of the cosmetics for skin regeneration and wound healing developed so far. Contains a large amount of artificially prepared compound components, and there is a problem in that the skin irritating side effects appear or the skin regeneration ability is limited.

The present invention was conceived to solve the above problems, the object of the present invention is a cosmetic composition for improving skin troubles comprising a complex extract consisting of a snapdragon extract, a sprig extract, and a scallop extract, sponge acupuncture powder and dietary sulfur as active ingredients Is to provide.

In order to solve the above problems, the present invention is a composite extract comprising a combination of two or more selected from the group consisting of a snapdragon extract, an extract of a gorilla extract, and an extract of nymph; Spongy powder; And dietary sulfur; provides a cosmetic composition for improving skin troubles comprising as an active ingredient.

According to the present invention, the composition may contain a snapdragon extract and a snapdragon extract in a weight ratio of 1:0.2-5.

According to the present invention, the composition may contain a snapdragon extract and a snapdragon extract in a weight ratio of 1:0.2-5.

According to the present invention, the composition may contain an extract of a stalk dog and an extract of yeokkwigi in a weight ratio of 1:0.2-5.

According to the present invention, the composition may contain a snapdragon extract, a snapdragon extract, and a yellowtail extract in a weight ratio of 1:0.15-6:0.15-6.

According to the present invention, the composition may contain a composite extract, a sponge powder and a dietary sulfur in a weight ratio of 1:0.15-6:0.15-6.

According to the present invention, the composition may be for skin moisturizing, skin soothing, skin inflammation and acne improvement.

According to the present invention, the composition is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension , Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray can be characterized by having a formulation selected from the group consisting of.

The composition according to the present invention can be used safely without side effects on the skin, and has excellent anti-inflammatory, skin regeneration and wound healing improvement effects, and is a cosmetic composition for improving skin problems such as skin moisturization, skin soothing, skin inflammation and acne. Can be used usefully.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.

In the present invention, a composite extract comprising a combination of two or more selected from the group consisting of a snapdragon extract, an extract of a gorilla extract, and an extract of nymph; Spongy powder; And dietary sulfur; provides a cosmetic composition for improving skin troubles comprising as an active ingredient.

The composition contains an extract derived from nature as an active ingredient, has excellent functionality and biosafety, has less skin irritation, and has excellent skin moisturization, skin soothing, skin irritation and skin trouble improvement effects such as acne.

The “comprised as an active ingredient” may exhibit skin trouble improvement effects, such as skin moisturizing, skin soothing, skin inflammation and acne improvement, pore contraction, sebum control, wrinkle improvement, skin elasticity improvement, etc. It may be meant to contain an effective amount of a certain degree.

Snapdragon used in the present invention ( Antirrhinum majus ) is a perennial plant belonging to the Scrophulariaceae family, and is native to Africa and southern Europe. The flower shape looks like a goldfish's mouth, so it is named snapdragon, and is called snapdragon in Europe and elsewhere. Snapdragon is widely used for ornamental purposes, but seeds, leaves, and flowers are also used as medicinal materials, and it is known to be rich in anthocyanins, linoleic acid, and oleic acid. In the present invention, the snapdragon may impart skin moisturizing, skin soothing, skin irritation and skin trouble improvement effects such as acne.

Referred dogs used in the present invention ( Hemistepita lyrata Bunge ) is a biennial herb belonging to the Asteraceae family (Compositae), and red-purple flowers bloom around May. The names of the herbal medicines of the dog are called Ihochae, Yagoma, Gomachae, and Namichae. They are 30~80cm in height and have fleshy conical roots, and the leaves are thin and long lanceolates. As an older brother, it grows wild in mountains and fields all over Korea. The outpost of Hemistepita lyrata Bunge has the effect of lowering fever, detoxifying, reducing swelling and eliminating blood stagnation, and is known to treat minor boils, traumatic bleeding, and fractures. In the present invention, the referred dog may impart skin moisturization, skin soothing, skin irritation and skin trouble improvement effects such as acne.

Persicaria used in the present invention hydropiper L.) is a one-year herb of the family Nodiridae, distributed over temperate and warmer regions of the northern hemisphere, and is often wild in wetlands or watersides. The stem grows to about 40~100cm, the branches are split a lot, there are no hairs, and the leaves running alternately are lanceolate without petioles, the ends are narrow, there are few hairs on the surface, the edges are flat, and the back is scattered. Branches from June to September, but reddish white-green flowers from the ends of the stems hang as water inflorescences. The fruit of the aquifer is wrapped in a calyx and ripened black. Flowers, leaves, and fruits can be used for food, but when chewed, they taste spicy. It has hemostasis and is used for uterine bleeding, hemorrhoids and other internal bleeding. Leaves and stems have excellent antibacterial effects, lower blood pressure, and strengthen the tension of the small intestine and uterus. In the present invention, it is possible to provide an effect of improving skin troubles such as skin moisturizing, skin soothing, skin inflammation and acne.

The extracts of snapdragon, gorilla, and nymph can be extracted with water, an organic solvent, or a combination thereof, and the organic solvent includes C1-C4 alcohol, acetone, chloroform, methylene chloride, ether, ethyl acetate, nucleic acid, or a combination thereof. May be used, but is not limited thereto. As the C1-C4 alcohol, methanol, ethanol, propanol and butanol may be exemplified, and ethanol is most preferred.

The extract can be prepared by any extraction method known to those skilled in the art in the field of extraction of natural products. For example, it may be extracted by performing one or more times using heat extraction, cold needle extraction, reflux cooling extraction, and ultrasonic extraction. In addition, the extraction temperature is not particularly limited as long as the useful component is not damaged, and may be, for example, 20 to 100°C, preferably about 50 to 90°C.

In the present invention, the extraction can be performed in the same manner as described above, and then further purified through a conventional purification process. For example, separation by using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc., can be further purified. In addition, it may be prepared and used in a powder state through a drying process such as distillation under reduced pressure, freeze drying, and spray drying.

First, since the cosmetic composition includes a fine needle powder, the penetration rate of the active ingredient of the composition into the skin may be significantly increased. The spicule is a composite material composed of calcium and silicate in the form of various fine needles collected from sponges inhabiting freshwater in Europe and Russia, and can generally be obtained in the form of a powder.

Since the fine needle has a fine needle-shaped structure and penetrates the skin layer to form a fine passage, the penetration effect of the applied cosmetic ingredient may be remarkably increased. In addition, the fine needle may promote skin regeneration of collagen, which acts on artificial wound healing, and may increase the biological activity by increasing the temperature of the skin tissue.

The fine needle is a material that is harmless to the human body, and is not particularly limited as long as it is a powdery material capable of stimulating the skin to form fine pores and being dissolved or discharged from the body when contacted with the skin.

The fine needle powder may be obtained from a sponge animal, and the sponge animal is Spongilla Lacustris L. lacustris L.), Spongilla fragilis Leidy ) and Ephydatia fluviatilis species.

The sponges are primitive marine organisms that do not have muscles, nerves or organs, and there are more than 5,000 species. The bone fragment is a tissue that functions as a skeleton having a needle shape existing in the body of an invertebrate, and mainly consists of silicate and calcium carbonate. The sponges may include uniaxial, triaxial, quaternary or multiaxial bone fragments containing calcium carbonate or silicic acid as a main component.

The sponges mainly live in the sea in the form of sponges of various colors such as orange or turquoise, but freshwater sponges also exist.

The sponge is an organism in the initial stage of tissue in a state in which cells are aggregated, and its body is composed of sponge fibers, spicules forming the body wall, and a group of cells bound to the bone fragment. The main component of the bone fragment is lime, silicic acid, or silica, and the size is around 100 μm, so it can be observed with a microscope.

The bone fragment is an effective means of skin regeneration, and can be used for correcting visual facial defects such as hyperpigmentation, fine wrinkles, sun damage and superficial scars, cotton cloths, and enlarged pores of various etiologies. In addition, it is used as a natural alternative approach to fine skin polishing and skin polishing, chemical dermabrasion, CO2 laser dermabrasion, and erbium laser dermabrasion, which are physical procedures to improve skin condition.

When the bone fragments contact and rub against the skin layer, they are physically separated to reduce the aggregation of keratinocytes, and the stratum corneum, sebum plugs, and keratinocytes are separated to form a fine passage, thus maximizing the penetration effect of cosmetic ingredients. It can also promote the regeneration of.

The average length of the fine needle powder may be 60 to 500 ㎛. If the average length of the fine needle powder is less than 60 μm, it cannot effectively penetrate into the skin to form pores, and if it is 500 μm, dispersibility may decrease or excessive irritation to the skin may be caused.

The dietary sulfur (MethylSulfonyl Methane) used in the present invention has a molecular formula of (CH ₃ ) ₂ SO ₂ , a molecular weight of 94, and a melting point of 108 to 109°C. The dietary sulfur plays a variety of roles, and in particular, plays a fundamental role in the human body. First of all, dietary sulfur has the function of detoxifying and smoothing blood circulation, and also maintains the balance of acidity and alkalinity in the body. In addition, dietary sulfur is an essential ingredient for producing collagen and keratin, which are essential proteins that maintain healthy hair, skin, hands, and toenails, and it acts as an essential element that helps maintain the elasticity and flexibility of all somatic tissues of the human body. do. In addition, the dietary sulfur is essential to maintain the permeability of cellular tissues, and proper permeability allows cells to absorb nutrients and discharge waste products. In particular, it is a substance that constitutes the basis of the sulfur-containing amino acids of methionine and cysteine, which are essential elements for the repair and treatment of most somatic tissues such as skin, blood vessels, organs, and joints. In addition, dietary sulfur is a free radical that is known to eliminate pollen and food allergies, and to eliminate hypersensitivity reactions to other medicines. In the present invention, the dietary sulfur soothes the skin, promotes collagen synthesis, strengthens the skin barrier, and can give tissue recovery effects.

The cosmetic composition of the present invention may contain a combination of the extract of snapdragon and gorilla extract, sponge powder, and dietary sulfur as active ingredients. At this time, it is preferable to contain the snapdragon extract and the extract of the dog species in a weight ratio of 1:0.2-5. If the ratio exceeds the upper limit or is less than the lower limit, the effect of improving skin problems such as skin moisturizing, skin soothing, skin inflammation and acne may be low.When included in the weight ratio, the synergistic effect between each active ingredient is maximized and excellent skin moisturizing, It has the effect of soothing skin, improving skin inflammation and acne, and can have excellent feeling of use of the formulation.

The cosmetic composition of the present invention may contain a combination of the extract of snapdragon and nymphatic, sponge powder and dietary sulfur as active ingredients. At this time, it is preferable to contain the snapdragon extract and the extract of nymph in a weight ratio of 1:0.2-5. If the ratio exceeds the upper limit or is less than the lower limit, the effect of improving skin problems such as skin moisturizing, skin soothing, skin inflammation and acne may be low.When included in the weight ratio, the synergistic effect between each active ingredient is maximized and excellent skin moisturizing, It has the effect of soothing skin, improving skin inflammation and acne, and can have excellent feeling of use of the formulation.

The cosmetic composition of the present invention may contain a combination of an extract of chinensis and yew, spongy powder and dietary sulfur as active ingredients. At this time, it is preferable to contain the above mentioned dog extract and the extract of Yeokki in a weight ratio of 1:0.2-5. If the ratio exceeds the upper limit or is less than the lower limit, the effect of improving skin problems such as skin moisturizing, skin soothing, skin inflammation and acne may be low.When included in the weight ratio, the synergistic effect between each active ingredient is maximized and excellent skin moisturizing, It has the effect of soothing skin, improving skin inflammation and acne, and can have excellent feeling of use of the formulation.

The cosmetic composition of the present invention may contain a combination of extracts of snapdragon, gorilla, and yeokwi, sponge needle powder, and dietary sulfur as active ingredients. At this time, it is preferable to contain the snapdragon extract, the gorilla extract, and the yeogwi extract in a weight ratio of 1:0.15-6:0.15-6. If the ratio exceeds the upper limit or is less than the lower limit, the effect of improving skin problems such as skin moisturizing, skin soothing, skin inflammation and acne may be low.When included in the weight ratio, the synergistic effect between each active ingredient is maximized and excellent skin moisturizing, It has the effect of soothing skin, improving skin inflammation and acne, and can have excellent feeling of use of the formulation.

The cosmetic composition of the present invention may contain a composite extract comprising a combination of two or more selected from the group consisting of a snapdragon extract, a gorilla extract, and an extract of nymph, and a sponge powder and dietary sulfur as active ingredients. At this time, it is preferable to contain the complex extract, sponge powder and dietary sulfur in a weight ratio of 1:0.15-6:0.15-6. If the ratio exceeds the upper limit or is less than the lower limit, the effect of improving skin moisturization, skin soothing, skin irritation and skin troubles such as acne, penetration effect of cosmetic ingredients, skin barrier strengthening and tissue recovery effect may be low, and when included in the weight ratio , Synergy effect between each active ingredient is maximized, so it has excellent skin moisturizing, skin soothing, skin inflammation and acne improvement effects, and the formulation can be excellent in feeling.

The cosmetic composition of the present invention may further include components included in a conventional cosmetic composition in addition to the above-described components. For example, it may further include one or more ingredients registered in the list of cosmetic ingredients registered with the Food and Drug Administration and the ICID (International Cosmetic Ingredients Collection). Specifically, it may further include one or more of a thickener, a moisturizing agent, a pH adjusting agent, an emulsifying agent, a colorant, a fragrance, a preservative, or a solvent.

A conventional thickener may be used. For example, carbomer, acrylate, ammonium acryloyldimethyltaurate/Vpicopolymer, acrylate/C10-30 alkyl acrylate crosspolymer, xanthan gum, and cellulose gum can be used.

The moisturizer may be a conventional one. For example, butylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, diglycerin, propanediol, 1,2-hexanediol, sodium hyaluronate, urea and trehalose (trehalose) etc. can be used.

The pH adjuster may be a conventional one. For example, organic acids such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid, glycolic acid, inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, sodium salt, potassium salt or ammonium salt of these acids, and arginine Among them, it can be used alone or in combination of two or more. In addition, triethanolamine, sodium chloride, sodium hydroxide, potassium hydroxide, and the like may be further included.

The emulsifier may be a conventional one. For example, polyoxyethylene sorbitan monolaurate, polyethylene glycol, and PEG-hydrogenated castor oil may be used, preferably Tween 20, which is polyoxyethylene sorbitan monolaurate, and a lipophilic surfactant. PEG-60 Hydrogenated Castor Oil can be mixed and used.

As the pigment, commonly used organic and inorganic pigments may be used. For example, muscovite mica, synthetic mica, firmness, kaolin, titanium dioxide, titanium oxide coated mica, aluminum powder, iron sulfate, black iron oxide, barium sulfate, and the like can be used.

As the perfume, ingredients commonly used in cosmetic compositions may be used.

Conventional preservatives may be used. For example, benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, methylparaben, ethylparaben, propylparaben, and the like can be used.

Conventional solvents may be used. For example, purified water, ethanol, cyclomethicone, or dimethicone may be used.

In addition to the aforementioned ingredients, the cosmetic composition of the present invention may additionally contain known natural extracts, vitamin derivatives, sequestering agents, etc., known to exert moisturizing and skin soothing effects.

In addition, the cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension , Emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray can be prepared in a formulation selected from the group consisting of, but limited thereto It does not become.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohard Propellants such as, but not limited to, locarbon, propane/butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as a carrier component. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

The cosmetic composition of the present invention can be applied after a dermatological laser treatment or to an acne area. When the cosmetic composition is applied, the skin regeneration and wound healing improvement effect is excellent, the stability is excellent, there is no side effect on the human body, and the feeling of use may be excellent.

[Example]

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not construed as being limited by these examples. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Manufacturing example 1: snapdragon, Designation dog And the preparation of the extract

(1) Extraction solvent (70% ethanol) was added to 100 g of a sample prepared by crushing freeze-dried snapdragon flowers, extracted for 90 minutes at 60°C, filtered under reduced pressure, and the filtrate was removed by ethanol using a vacuum concentrator and then freeze-dried to powder. Obtained a snapdragon extract.

(2) Extraction solvent (70% ethanol) was added to 100 g of dry powdered dogwood, extracted at 60°C for 90 minutes, filtered under reduced pressure, and the filtrate was removed with ethanol using a vacuum concentrator and freeze-dried to obtain a powdered dogwood extract.

(3) Extraction solvent (70% ethanol) was added to 100 g of a sample prepared by pulverizing the dried yellow bean, extracted at 60°C for 90 minutes, filtered under reduced pressure, and the filtrate was removed by ethanol using a vacuum concentrator and then freeze-dried to powder. To obtain an extract.

Manufacturing example 2: Fine needle Powder manufacturing

Dried sponge ( Spongilla lacustris ) 200 g of a natural sponge obtained from 0.5N-sodium hydroxide solution was added to 800 ml, followed by mixing and pulverization by an ultrasonic process. After stirring, impurities were removed through centrifugation, and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A trace amount of sodium hydroxide was removed by a chemical reaction with 800 ml of a 2%-L-ascorbic acid solution, and the over-input L-ascorbic acid solution was removed and dried to obtain a fine needle powder from which purified water was removed.

Example And Comparative example

In order to verify the effect of improving skin troubles of the snapdragon extract obtained through Preparation Example 1, the extracts of chinensis, and the extract, the fine needle powder obtained through Preparation Example 2, and the dietary sulfur, samples were mixed as shown in Tables 1 to 7 below. Examples and comparative examples were set.

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 1-1 150 150 - 100 100 2-1 150 - 150 100 100 3-1 - 150 150 100 100

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 1-1 150 150 - 100 100 1-2 200 100 - 100 100 1-3 250 50 - 100 100 1-4 275 25 - 100 100 1-5 100 200 - 100 100 1-6 50 250 - 100 100 1-7 25 275 - 100 100

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 2-1 150 - 150 100 100 2-2 200 - 100 100 100 2-3 250 - 50 100 100 2-4 275 - 25 100 100 2-5 100 - 200 100 100 2-6 50 - 250 100 100 2-7 25 - 275 100 100

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 3-1 - 150 150 100 100 3-2 - 200 100 100 100 3-3 - 250 50 100 100 3-4 - 275 25 100 100 3-5 - 100 200 100 100 3-6 - 50 250 100 100 3-7 - 25 275 100 100

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 4-1 150 150 150 100 100 4-2 100 100 100 100 100 4-3 150 75 75 100 100 4-4 200 50 50 100 100 4-5 225 37.5 37.5 100 100 4-6 250 25 25 100 100 4-7 75 150 75 100 100 4-8 50 200 50 100 100 4-9 37.5 225 37.5 100 100 4-10 25 250 25 100 100 4-11 75 75 150 100 100 4-12 50 50 200 100 100 4-13 37.5 37.5 225 100 100 4-14 25 25 250 100 100

Sample [content (parts by weight)] Example snapdragon Designation dog Yes Fine needle Dietary sulfur 5-1 100 100 100 100 100 5-2 100 100 100 125 75 5-3 100 100 100 150 50 5-4 100 100 100 175 25 5-5 100 100 100 187.5 12.5 5-6 100 100 100 75 125 5-7 100 100 100 50 150 5-8 75 150 75 25 175 5-9 50 200 50 12.5 187.5

Sample [content (parts by weight)] Comparative example snapdragon Designation dog Yes Fine needle Dietary sulfur One - - - 250 250 2 300 - - 100 100 3 - 300 - 100 100 4 - - 300 100 100

Experimental example 1: skin safety test

In order to verify the skin safety of the samples of Examples and Comparative Examples, MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT). Samples of Examples and Comparative Examples were suspended in purified water for each concentration of 0, 1, 5, 10, 20, 50 and 100 mg/L, and then cell viability was measured. Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT{3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide} reagent. Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were aliquoted into 96-well plates at a concentration of 1×10 ⁴ cells/well, respectively, and cultured at 37°C and 5% CO ₂ for 48 hours. I did. The culture medium was removed, and the samples were cultured in a medium treated by concentration for 48 hours, and then the medium was removed and washed repeatedly with PBS (Phosphate buffered saline). MTT was dissolved in PBS at 5mg/mL, 50L was added, and incubated at 37°C and 5% CO2 for 48 hours. 100L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and absorbance was measured at 540nm.

As a result of the measurement, cytotoxicity was not observed at all concentrations of each sample, and through this, it was confirmed that the components used in the present invention are not only harmless to the human body, but also have excellent human safety.

Experimental example 2: Skin soothing Effectiveness evaluation

The skin soothing effect of the cosmetic composition formulated samples of Examples and Comparative Examples was evaluated. Each sample was formulated with a cream having the same composition, and only samples of Examples and Comparative Examples were different. After inducing skin irritation through a tape stripping method on the inner arm of 50 women with skin sensitive to irritation among 19-26 years old, the formulated Examples 1-1 and 2- The samples of 1, 3-1, 4-2 and Comparative Examples 1 to 4 were applied twice a day to the face and front part for 1 month, and the degree of improvement was determined according to the opinion of the user such as itching, itching, redness, and erythema. The results are shown in Table 8 below.

division 1 week 2 weeks 3 weeks 4 weeks Example 1-1 + ++ +++ +++ Example 2-1 + ++ +++ +++ Example 3-1 + ++ ++ +++ Example 4-2 ++ +++ +++ +++ Comparative Example 1 ± ± ± ± Comparative Example 2 ± ± + + Comparative Example 3 ± ± + + Comparative Example 4 ± ± ± +

<Evaluation criteria>

+++: Has a very good improvement effect / ++: Has a significant improvement effect / +: Has a slight improvement effect / ±: No improvement effect, but does not worsen / -: No improvement effect, rather weakens

As shown in Table 8 above, a combination of two or more of the snapdragon extract, the gorilla extract, and the yeokkwi extract according to the present invention, and samples containing spongy powder and dietary sulfur (Example 1-1, Example 2-1, Example 3-1 and Example 4-2) contains only spongy sprout powder and dietary sulfur (Comparative Example 1), or a sample containing one extract of snapdragon extract, gorilla extract, and yeokkwi extract, sponge acupuncture powder and dietary sulfur It was confirmed that the skin soothing effect was remarkably superior compared to (Comparative Examples 2 to 4).

Experimental example 3: Evaluation of skin elasticity improvement effect

The effect of improving skin elasticity of the cosmetic compositions formulated samples of Examples and Comparative Examples was evaluated. Each sample was formulated with a cream having the same composition, and only samples of Examples and Comparative Examples were different. Healthy women 20 years of age or older (average age 37 years old) were divided into groups under conditions of temperature 24 to 26°C and humidity of 75%, and the creams of Examples and Comparative Examples were applied, respectively. The formulated cream was applied twice a day (morning and evening) around the eyes for 12 weeks, and skin elasticity was measured using a skin elasticity meter (Cutometer MPA580, Conrage + Khazaka, Germany). The test result was described as the value of R8 (R8 (week 12)-R8 (week 0)) of the cutometer MPA580, and the R8 value indicates the property of skin viscoelasticity, and the measurement results are shown in Table 9 below.

division Skin elasticity improvement effect Example 1-1 0.88 Example 2-1 0.87 Example 3-1 0.84 Example 4-2 0.93 Comparative Example 1 0.43 Comparative Example 2 0.52 Comparative Example 3 0.54 Comparative Example 4 0.49 Positive control (vitamin C) 0.91 Negative control (no treatment) 0.29

From the results shown in Table 9, a combination of two or more of the snapdragon extract, the gorilla extract, and the extract of the snail extract according to the present invention, and a sample containing spongy sprout powder and dietary sulfur (Example 1-1, Example 2-1, Example 3-1 and Example 4-2) contains only spongy sprout powder and dietary sulfur (Comparative Example 1), or contains one extract of snapdragon extract, gorilla extract, and scallop extract, and sponge powder and dietary sulfur. It was confirmed that the skin elasticity improvement effect was significantly improved compared to the samples (Comparative Examples 2 to 4).

Experimental example 4: atopic skin improvement Clinical trial of effectiveness

In order to confirm the effect of improving skin itching of the cosmetic compositions formulated samples of Examples and Comparative Examples, clinical trials were conducted as follows on patients with atopic disease. Each sample was formulated with a cream having the same composition, and only samples of Examples and Comparative Examples were different.

The clinical trial was conducted in 80 patients between 10 and 60 years old with symptoms of skin itching due to atopy (Example 1-1, Example 1-2, Example 1-3, Example 4-2, and Comparative Examples 1 to 4, 10 patients each), and steroids related to itching and antihistamine drugs were not used at all from 2 weeks before the start of the experiment.

And all the test subjects were applied to the itchy area three times a day for 4 weeks, and then the condition after 1 month was evaluated by the items shown in Table 10 below, and the results are shown in Table 11.

Experiment item Subjective score

Skin itching improvement effect
a: greatly improved
b: improved
c: no difference
d: worsened
e: very worse

Very improved Improved No difference Worsened Very worse Example 1-1 3 people 6 people 1 person - - Example 2-1 2 people 6 people 2 people - - Example 3-1 1 person 5 people 3 people - - Example 4-1 6 people 4 people - - - Example 4-2 4 people 6 people Comparative Example 1 - 7 people 3 people - Comparative Example 2 - 3 people 6 people 1 person - Comparative Example 3 - 2 people 7 people 1 person - Comparative Example 4 - 1 person 7 people 2 people -

As shown in Table 11 above, a combination of two or more of the snapdragon extract, the gorilla extract, and the extract of the snail extract according to the present invention, and a sample containing spongy sprout powder and dietary sulfur (Example 1-1, Example 2-1, While most of the subjects using Example 3-1 and Example 4-2) answered that the skin itching symptoms were greatly improved or improved, they contained only spongy sprout powder and dietary sulfur (Comparative Example 1), or snapdragon extract, gorilla extract and Subjects who used one of the extracts of the extract of nymph extract and samples containing spongy sprout powder and dietary sulfur (Comparative Examples 2 to 4) answered that there was some improvement, but most of them answered that there was no difference or that the symptoms of itching were worse. . In addition, as the content of the complex extract increased, the number of subjects who answered that it was greatly improved or improved increased (compare Example 4-1 and Example 4-2).Through these results, the useful ingredient according to the present invention plays a large role in relieving skin itching. It was confirmed that.

Experimental example 5: Infection of atopic dermatitis Clinical trial for symptoms

In order to confirm the improvement effect of the cosmetic compositions formulated with the samples of Examples and Comparative Examples on the infection symptoms of atopic dermatitis, a clinical trial was conducted as follows for patients with atopic disease. Each sample was formulated with a cream having the same composition, and only samples of Examples and Comparative Examples were different. The clinical trial was conducted in 40 patients with atopic skin infection between 10 and 60 years old (Examples 1-1, 1-2, Example 1-3, Example 4-2, and Comparative Examples 1 to 4 All 5 subjects) were subjects, and steroids and antihistamine drugs related to atopic treatment were not used from 2 weeks before the start of the experiment.

And all test subjects were applied 3 times a day for 4 weeks on areas with skin infections, and then the condition after 1 month was evaluated by the items shown in Table 12 below, and the results are shown in Table 13.

Experiment item Subjective score
Atopic skin infection symptom improvement effect a: greatly improved
b: improved
c: no difference
d: worsened
e: very worse

Very improved Improved No difference Worsened Very worse Example 1-1 2 people 2 people 1 person - - Example 2-1 1 person 2 people 2 people - - Example 3-1 1 person 2 people 2 people - - Example 4-1 4 people 1 person - - - Example 4-2 2 people 3 people Comparative Example 1 - - 3 people 2 people - Comparative Example 2 - 2 people 3 people - - Comparative Example 3 - 1 person 3 people 1 person - Comparative Example 4 - 1 person 3 people 1 person -

As shown in Table 13 above, a combination of two or more of the snapdragon extract, the gorilla extract, and the yeokkwi extract according to the present invention, and a sample containing a sponge powder and dietary sulfur (Example 1-1, Example 2-1, While most of the subjects using Example 3-1 and Example 4-2) answered that seborrheic inflammation occurred in the affected area was greatly improved or improved, only sponge powder and dietary sulfur were included (Comparative Example 1), or snapdragon extract, The subjects who used one of the extracts and extracts of the extract and samples containing spongy sprout powder and dietary sulfur (Comparative Examples 2 to 4) had some improvement, but most answered that there was no difference, or rather, symptoms worsened. I did. In addition, as the content of the complex extract increased, the number of subjects who answered that it was greatly improved or improved (compare Example 4-1 and Example 4-2) was increased.Through these results, the useful ingredient according to the present invention was not affected by skin infection symptoms caused by atopy. It was confirmed that it plays a big role in improvement.

Experimental example 6: acne improvement Clinical trial of effectiveness

The effect of improving acne of the cosmetic compositions formulated samples of Examples and Comparative Examples was evaluated. Each sample was formulated with a cream having the same composition, and only samples of Examples and Comparative Examples were different. Clinical trials were 40 of 19-26 year old women with acne (Example 1-1, Example 1-2, Example 1-3, Example 4-2, and Comparative Examples 1 to 4 all 5 patients each) The subjects were, and the condition after being used in the same way as a daily lotion for each 4 weeks was evaluated by the items shown in Table 14 below, and the results are shown in Table 15.

Experiment item Subjective score

Acne improvement effect
a: greatly improved
b: improved
c: no difference
d: worsened
e: very worse

Very improved Improved No difference Worsened Very worse Example 1-1 1 person 3 people 1 person - - Example 2-1 1 person 2 people 2 people - - Example 3-1 - 3 people 2 people - - Example 4-1 3 people 2 people - - - Example 4-2 1 person 4 people Comparative Example 1 - - 3 people 2 people - Comparative Example 2 - 1 person 4 people - - Comparative Example 3 - 1 person 3 people 1 person - Comparative Example 4 - 1 person 2 people 2 people -

As shown in Table 15 above, a combination of two or more of the snapdragon extract, the squirrel extract, and the extract of the snail extract according to the present invention, and a sample containing spongy sprout powder and dietary sulfur (Example 1-1, Example 2-1, While most of the subjects using Example 3-1 and Example 4-2) answered that their acne symptoms were greatly improved or improved, they contained only spongy sprout powder and dietary sulfur (Comparative Example 1), or snapdragon extract, gorilla extract, and nymph. Subjects who used one of the extracts and samples containing spongy sprout powder and dietary sulfur (Comparative Examples 2 to 4) answered that there was some improvement, but most of them answered that there was no difference or that symptoms were worsening. In addition, as the content of the complex extract increased, the number of subjects who answered that it was greatly improved or improved increased (compare Example 4-1 and Example 4-2). It was confirmed to play a role.

Experimental example 7: Measurement of inhibitory effect on NO (nitric oxide) production

Samples of Examples and Comparative Examples were tested for inhibiting NO production, which acts as a major factor in the occurrence of skin inflammation. First, RAW 264.7 cells, which are mouse macrophages, were cultured in 10% FBS DMEM medium, and then dispensed into a 24-well plate at 4×10 ⁵ cells/well and cultured in a 5% CO ₂ constant temperature and humidity chamber for 24 hours. After removing the medium, the samples of Examples and Comparative Examples and FBS free DMEM containing methylarginine (LNMMA) were treated, and after 30 minutes, NO was induced with lipopolysaccharide (LPS) and cultured for 24 hours. Using the NO assay kit, the amount of NO in the sample compared to the LPS treatment was calculated to evaluate the NO generation inhibition rate, and the results are shown in Table 16 below.

division Concentration (ppm) NO production inhibition rate (%) Example 1-1 10 68.7 Example 1-2 10 71.4 Example 1-3 10 72.3 Example 1-4 10 69.9 Example 1-5 10 67.4 Example 1-6 10 65.3 Example 1-7 10 63.8 Example 2-1 10 67.4 Example 2-2 10 69.2 Example 2-3 10 70.1 Example 2-4 10 68.6 Example 2-5 10 66.5 Example 2-6 10 63.9 Example 2-7 10 61.7 Example 3-1 10 67.2 Example 3-2 10 68.9 Example 3-3 10 69.6 Example 3-4 10 68.1 Example 3-5 10 66.1 Example 3-6 10 63.0 Example 3-7 10 60.8 Example 4-1 10 87.3 Example 4-2 10 78.6 Example 4-3 10 77.0 Example 4-4 10 75.8 Example 4-5 10 75.5 Example 4-6 10 74.9 Example 4-7 10 73.8 Example 4-8 10 72.2 Example 4-9 10 71.7 Example 4-10 10 70.3 Example 4-11 10 70.1 Example 4-12 10 68.4 Example 4-13 10 65.7 Example 4-14 10 65.1 Example 5-1 10 78.6 Example 5-2 10 75.3 Example 5-3 10 73.7 Example 5-4 10 68.4 Example 5-5 10 66.6 Example 5-6 10 76.5 Example 5-7 10 74.8 Example 5-8 10 68.2 Example 5-9 10 67.3 Comparative Example 1 10 41.8 Comparative Example 2 10 52.3 Comparative Example 3 10 51.7 Comparative Example 4 10 50.5 LNMMA 10 46.3

As shown in Table 16 above, a combination of two or more of the snapdragon extract, the gorilla extract, and the yeokkwi extract according to the present invention, and a sample containing spongy sprout powder and dietary sulfur (Example 1-1, Example 2-1, Example 3-1 and Example 4-2) contain only representative NO production inhibitors LNMMA, sponge powder and dietary sulfur (Comparative Example 1), or extract of one of snapdragon extract, gorilla extract, and spongy extract It was confirmed that the anti-inflammatory effect was very excellent as it showed a better inhibition of NO generation than the samples containing powder and dietary sulfur (Comparative Examples 2 to 4). In addition, it was found that as the content of the complex extract increased (compare Example 4-1 and Example 4-2), the NO generation inhibition rate increased.

Next, in the case of the combination of the snapdragon extract and the dogfish extract and a sample containing spongy sprout powder and dietary sulfur (Examples 1-1 to Example 1-7), NO generation in both the composition of the mixture of the snapdragon extract and the dogfish extract The inhibition rate was increased, particularly in the case of Examples 1-1 to 1-4, the inhibition rate of NO generation was excellent, and among them, it was found that the inhibition rate of NO generation was relatively high in Examples 1-2 and Example 1-3. .

Next, in the case of a combination of a snapdragon extract and a yellowtail extract and a sample containing a spongy sprout powder and dietary sulfur (Examples 2-1 to 2-7), NO generation in both the composition of the mixture of the snapdragon extract and the yellowtail extract The inhibition rate was increased, particularly in the case of Examples 2-1 to 2-4, the inhibition rate of NO generation was excellent, and among them, it was found that the inhibition rate of NO generation was relatively high in the case of Example 2-2 and Example 2-3. .

Next, in the case of a sample containing a combination of a spongy dog extract and a sprout extract and a spongy sprout powder and dietary sulfur (Example 3-1 to Example 3-7), NO is produced in both the composition of the mixture of the spicy sprout extract and the sprout extract. The inhibition rate was increased, particularly in the case of Examples 3-1 to 3-4, the inhibition rate of NO generation was excellent, and among them, it was found that the inhibition rate of NO generation was relatively high in the case of Example 3-2 and Example 3-3. .

Next, in the case of a sample containing a combination of snapdragon extract, gorilla extract, and nymph extract and spongy needle powder and dietary sulfur (Examples 4-1 to 4-14), a mixture of snapdragon extract, gorilla extract and yeomangi extract In all one composition, the inhibition rate of NO generation was increased, and it was found that the rate of inhibition of NO generation was improved compared to the case of combining two of the snapdragon extract, the gorilla extract, and the yellowtail extract in most cases (Examples 1 to 3). In the case of 1 to 4-3, it was found that the NO generation inhibition rate was relatively high.

Finally, in the case of a sample containing a combination of a snapdragon extract, an extract of a gorilla extract, and an extract of a yellowtail extract, but with a different composition of the mixture of spongy sprout powder and dietary sulfur (Examples 5-1 to 5-9), the NO production inhibition rate was Although increased, in particular, in the case of Examples 5-1 to 5-3 and Examples 5-6 to 5-7, the NO generation inhibition rate was found to be relatively high.

Experimental example 8: MMP -1 (Matrix metalloproteinase -1) and PGE2 ( Prostagladin E2) Measurement of expression inhibition effect

The samples of Examples and Comparative Examples were subjected to ELISA (Enzyme Linked Immuno Sorbent Assay) to measure the effect of inhibiting the expression of MMP-1 and PGE2, which act as major factors in the occurrence of skin inflammation. First, RAW264.7 cells, which are mouse macrophages, were cultured in 10% FBS DMEM medium, and distributed in a 24-well plate at 1×10 ⁵ cells/well and cultured in a 5% CO ₂ thermo-hygrostat for 24 hours. Thereafter, the medium was exchanged and the samples of Examples and Comparative Examples were treated and cultured for an additional 24 hours, followed by treatment with a primary antibody (monoclonal antibody) and reacted at 37° C. for 90 minutes. Next, the secondary antibody was treated with an anti-mouse immunoglobulin (alkaline phosphatase conjugated anti-mouse IgG) and reacted for 90 minutes, washed with a buffer solution, and then an alkaline phosphatase substrate solution (1 mg/ml in a diethanolamine buffer solution). p-nitrophenyl phosphate) was reacted at room temperature for 30 minutes, and absorbance was measured at 405 nm using an absorbance photometer. The absorbance of the cell culture solution not treated with the samples of Examples and Comparative Examples was used as a control, and the effect of inhibiting the expression of MMP-1 and PGE2 was calculated by Equation 1 below, and the results are shown in Table 17 below.

[Equation 1]

MMP-1 and PGE2 inhibition rate (%) = (A-B)/A*100

A: absorbance of a well without adding a sample

B: absorbance of the well to which the sample was added

division MMP-1 expression inhibition rate (%) PGE2 expression inhibition rate (%) Example 1-1 84.7 87.4 Example 1-2 87.3 90.1 Example 1-3 88.2 90.9 Example 1-4 85.0 88.4 Example 1-5 83.6 86.2 Example 1-6 81.4 84.7 Example 1-7 80.9 83.9 Example 2-1 83.6 86.7 Example 2-2 86.8 89.2 Example 2-3 87.0 90.1 Example 2-4 83.8 86.3 Example 2-5 81.8 84.4 Example 2-6 80.9 83.7 Example 2-7 79.7 81.5 Example 3-1 83.4 86.2 Example 3-2 84.5 87.1 Example 3-3 85.3 88.0 Example 3-4 84.2 87.0 Example 3-5 82.7 85.4 Example 3-6 80.4 83.1 Example 3-7 78.6 81.5 Example 4-1 94.3 95.2 Example 4-2 90.5 91.8 Example 4-3 89.4 90.0 Example 4-4 88.8 89.9 Example 4-5 87.9 88.7 Example 4-6 87.6 88.5 Example 4-7 87.8 88.7 Example 4-8 86.6 87.4 Example 4-9 86.3 87.2 Example 4-10 85.8 87.1 Example 4-11 85.7 86.9 Example 4-12 84.3 85.2 Example 4-13 82.0 85.3 Example 4-14 81.8 84.7 Example 5-1 90.5 91.8 Example 5-2 88.3 89.9 Example 5-3 88.2 88.5 Example 5-4 85.6 86.4 Example 5-5 83.7 84.2 Example 5-6 88.8 88.6 Example 5-7 88.4 88.5 Example 5-8 85.6 85.7 Example 5-9 84.9 83.8 Comparative Example 1 51.4 52.5 Comparative Example 2 66.7 67.8 Comparative Example 3 65.4 66.6 Comparative Example 4 65.8 66.0 Control - -

As shown in Table 17 above, a combination of two or more of the snapdragon extract, the gorilla extract, and the yeokkwi extract according to the present invention, and a sample containing a sponge powder and dietary sulfur (Example 1-1, Example 2-1, Example 3-1 and Example 4-2) contains only spongy sprout powder and dietary sulfur (Comparative Example 1), or a sample containing one extract of snapdragon extract, gorilla extract, and yeokkwi extract, sponge acupuncture powder and dietary sulfur Compared to (Comparative Examples 2 to 4), it was confirmed that the effect of preventing skin inflammation and trouble by effectively inhibiting the expression of MMP-1 and PGE2, which are inflammatory factors of the skin, is very excellent. In addition, it was found that as the content of the complex extract increased (compare Example 4-1 and Example 4-2), the expression inhibition rate increased.

Next, in the case of a sample containing a combination of a snapdragon extract and an extract of a blackfish and a sponge needle powder and a dietary sulfur (Example 1-1 to Example 1-7), MMP- The inhibition rate of expression of 1 and PGE2 was increased, and in particular, in the case of Examples 1-1 to 1-4, the expression inhibition rate of MMP-1 and PGE2 was excellent, and among them, Examples 1-2 and 1-3 In the case, the inhibition rate of MMP-1 and PGE2 expression was relatively high.

Next, in the case of a combination of a snapdragon extract and a yellowtail extract and a sample containing spongy sprout powder and dietary sulfur (Examples 2-1 to 2-7), MMP- The inhibition rate of expression of 1 and PGE2 was increased. In particular, in the case of Examples 2-1 to 2-4, the inhibition rate of expression of MMP-1 and PGE2 was excellent, and among them, Examples 2-2 and 2-3 In the case, the inhibition rate of MMP-1 and PGE2 expression was relatively high.

Next, in the case of a sample containing a combination of a spongy dog extract and a sprout extract and a spongy sprout powder and dietary sulfur (Example 3-1 to Example 3-7), MMP- The inhibition rate of expression of 1 and PGE2 was increased, and in particular, in the case of Examples 3-1 to 3-4, the expression inhibition rate of MMP-1 and PGE2 was excellent, and among them, Examples 3-2 and 3-3. In the case, the inhibition rate of MMP-1 and PGE2 expression was relatively high.

Next, in the case of a sample containing a combination of snapdragon extract, gorilla extract, and nymph extract and spongy needle powder and dietary sulfur (Examples 4-1 to 4-14), a mixture of snapdragon extract, gorilla extract and yeomangi extract In both compositions, the inhibition rate of expression of MMP-1 and PGE2 was increased, and the rate of inhibition of expression of MMP-1 and PGE2 was improved compared to the case of combining two of the snapdragon extract, the gorilla extract, and the red bean extract (Examples 1 to 3). In particular, in the case of Examples 4-1 to 4-3, the rate of inhibition of expression of MMP-1 and PGE2 was relatively high.

Finally, in the case of a sample containing a combination of a snapdragon extract, an extract of a gorilla extract, and an extract of a nymph extract, but in the case of a sample having a different composition of the mixture of sponge spit powder and dietary sulfur (Examples 5-1 to 5-9), MMP-1 and Although the rate of inhibition of expression of PGE2 increased, in particular, in Examples 5-1 to 5-3 and Examples 5-6 to 5-7, the inhibition rate of expression of MMP-1 and PGE2 was relatively high.

As described above, specific parts of the present invention have been described in detail, and it is obvious that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereby. something to do. Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

Complex extracts including snapdragon extract, gorilla extract, and nymph extract;
Spongy powder; And
Contains dietary sulfur; as an active ingredient,
A cosmetic composition for improving skin troubles, characterized in that it contains the snapdragon extract, the gorilla extract, and the yeokki extract in a weight ratio of 1:0.15-6:0.15-6. delete delete delete delete The method of claim 1,
The composition is a cosmetic composition for improving skin problems, characterized in that it contains a complex extract, sponge powder and dietary sulfur in a weight ratio of 1:0.15-6:0.15-6 The method of claim 1,
The composition is a cosmetic composition for improving skin troubles, characterized in that for skin moisturizing, skin soothing, skin inflammation and acne improvement. The method of claim 1,
The composition is a solution, external ointment, cream, foam, nutritional lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion, paste , Gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, a cosmetic composition for improving skin trouble, characterized in that it has a formulation selected from the group consisting of a patch and spray.