KR20070070659A - Cosmetic composition for combatting aging the skin - Google Patents

Abstract

A cosmetic composition for preventing skin aging is provided to improve blood flow by enlarging the capillary vessel, activate cell growth, inhibit oxidation, and reduce skin irritation. The cosmetic composition for preventing skin aging comprises 0.0001-30 wt.% of extracts of the medicinal herb complex containing 1-20 parts by weight of Zizyphus jujuba Miller(Z. vulgaris Lam. var. spinosus Bunge), 1-20 parts by weight of Ganoderma lucidum, 1-20 parts by weight of Nelumbo nucifera Gaertner fruits, 1-20 parts by weight of Euphoria longana, 1-20 parts by weight of Coptis chinensis, 1-20 parts by weight of Prunus persica seed, 1-20 parts by weight of Carthamus tinctorius, 1-20 parts by weight of Angelica gigas, 1-20 parts by weight of Paeonia suffruticosa Andrews, and 1-20 parts by weight of Bambusae Caulis in taeniam, wherein the cosmetic composition is formulated as lotion, essence, cream, pack, gel, ointment, patch or spray.

Description

Cosmetic composition having anti-aging effect of skin {Cosmetic composition for combatting aging the skin}

1 is a blood flow improvement efficacy measurement results of the human extract.

Figure 2 is the result of measuring the cell proliferation activity potency of the extract.

3 is a result of measuring the antioxidant efficacy of the extract.

The present invention relates to a cosmetic composition having an anti-aging effect of skin, and more particularly, by containing a herbal mixture mixed with Sanjoin, ganoderma lucidum, lotus root, longan meat, rhubarb, cabbage, safflower, donkey, bark skin, and kill extract as an active ingredient. In addition, the present invention relates to a cosmetic composition for preventing skin aging, which is safe without irritation to the skin, and capillaries are expanded upon application of the skin, thereby improving blood flow and having excellent cell proliferation activity and antioxidant efficacy.

Cosmetics make the skin beautiful in appearance and its purpose is to maximize beauty. Raw materials with various effects such as whitening, wrinkle improvement and skin protection are screened and added to cosmetics. Among them, improvement of skin blood flow makes dull and elastic skin fresh and clear skin, making healthy skin that stands out.

However, the substance having the effect of improving blood flow is not known to be extremely limited or effective, and a small number of substances used at present are chemically synthesized and used.

In addition, the skin of the human body is a part directly exposed to the external environment, and when exposed to excessive ultraviolet rays or contaminants, free radicals and free radicals are generated, and erythema, edema, rashes and tingling are caused by the generated free radicals or free radicals. Skin irritation and inflammatory reactions, such as itching, are caused. Skin troubles caused by these causes are not only aesthetic problems, but also substances produced during the inflammatory process may cause pigmentation of the skin and accelerate the breakdown of skin elastic fibers, thereby affecting the increase of skin wrinkles. It is known to lead to skin aging.

As the skin ages, keratinization and fusion of epidermal cells are gradually slowed down, the epidermal cell layer becomes thinner, the stratum corneum becomes thicker, and the boundary line with the dermis layer becomes smoother. In the dermis, fibroblasts age to decrease the ability to produce fibers and substrates, and skin cells rapidly increase peroxide levels in cells as they age, and are susceptible to various pollutants and stress. The components of cells and cell membranes are changed to peroxides, protein damage occurs, and the cells are unable to perform their functions smoothly, which lowers the metabolic activity of the cells, and wrinkles are caused by these functional and structural damages. Further promoted. Blocking the steps involved in this skin aging mechanism can fundamentally prevent skin aging.

Thus, efforts to find a substance that can protect against aging have been continuously progressed in the past, and researches to search for new raw materials with improved blood flow, cell active proliferation, and antioxidant efficacy have been actively conducted at home and abroad.

The present invention has been made to solve the problems of the prior art as described above, the present invention is not only safe without irritation to the skin using natural materials, improve skin blood flow during skin application, has a cell proliferation activity and antioxidant An object of the present invention is to provide an excellent anti-aging cosmetic composition.

In order to achieve the above object, the present invention

Sanjoin, ganoderma lucidum, lotus root, longan meat, rhubarb, cabbage, safflower, donkey, bark skin, kill extract provides herbal composition (hereinafter referred to as 'hwahwa extract') comprising an extract as an active ingredient do.

The sanjoin, ganoderma lucidum, lotus root, longan meat, yellow lotus, doin, safflower, donkey, bark skin, kill contained in the extract is characterized in that the mixture is extracted in an amount of 1 to 20 parts by weight, respectively.

Sanjoin, ganoderma lucidum, lotus root, longan, sulfur, doin, safflower, donkey, bark skin, kill the content of the mixed extract provides a cosmetic composition for preventing skin aging, characterized in that 0.0001 to 30% by weight relative to the total weight of the cosmetic composition. .

Hereinafter, the present invention will be described in detail.

The present inventors have conducted studies on various substances to find excellent blood flow improvement, cell proliferation activity, and antioxidant potency from natural natural products, which have been conventionally used as herbal medicines. , Safflower, Angelica, bark skin, kill the extract extracted (hereinafter referred to as "hwahwa extract") was confirmed that the blood flow improvement, cell proliferation activity, antioxidant effect in the skin was excellent and completed the present invention.

Sanjoin used in the present invention (Zenzoyphus jujuba Miller (Z. vulgaris Lam. Var. Spinosus Bunge)) grows in the mountains and the shape is not jujube tree, but the fruit is very small than jujube, lunar 8 Fruits are harvested in January and the seeds (without flesh) are removed and used. The nature of the barberry seed is flat, tasteful, and nontoxic, and it helps relieve insomnia due to confession, painful symptoms of navel up and down, bloody diarrhea, and cold sweat. If your blood does not return well to the rain, you can not sleep comfortably even if you are lying down. Of course, it is a good idea to look at your heart and rain largely. Then your blood will return to the rain and your five bowels will be comfortable. It will come well. If you use this medicine, break the mejujube seed and use the inner kernel.If you have a lot of sleep, use it as raw food. If you don't sleep, stir-fry it, cook it for about a day, then remove the shells and sharp parts and grind it finely. It is also called barberry, bergamot, and barberry.

Ganoderma lucidum (靈芝 Scientific name Ganoderma lucidum) used in the present invention is also called bullocho. It occurs in the roots of hardwoods in summer and also sprouts on the ground. It is a year-round mushroom that has the same gloss as lacquer on its lampshade and bag surface. Gad is semicircular, elongated or fan-shaped, with flat surface and concentric vents. At first, it turns into egg white, yellowish brown or reddish brown and becomes chestnut brown when old. The flesh is cork, and the upper and lower two layers, the upper layer is almost white, and the lower layer of the tube is yellow. The underside of the shade is yellowish white with 5-10 mm length of holes lined in one layer, and the tool is round. The sack is 3 ~ 15 × 1 ~ 2 cm, reddish brown to dark brown, wrapped in hard cuticle, slightly curved. Spores are bilayer, spore pattern light brown. In oriental medicine, it has an effect such as tonic haejongjong (消腫) is used for nervous breakdown, heart disease, high blood pressure, various cancers. The mushrooms are covered with hard skin and have varnish like varnish. They are called mushrooms in Japan, and in China, ganoderma lucidum.

Used in the present invention, yeonjakyuk, yeonbab is called yeonjakyuk (蓮 子 肉) in one shot. The lotus flower (Nelumbo nucifera Gaertner), which symbolizes the Buddha, is the positivization of pistils and stamens in one flower. It consists of about 300 stamens and about 40 pistils and irises in one flower, and the petals are white long ovals. It has 18 to 26 petals, and after fertilization, it produces 1.5 cm in size. It contains 15 to 25 black seeds, and the life of ripe seeds is about 500 years. When the petals fall, the honeycomb-shaped fruit ripens brown in October, and contains oval seeds in it. When the lotus flower is lost, the fruit is called lotus (蓮 實) and is used to decorate the house or use it as a medicine. In addition, roots with multiple holes are called lotus root, and they are enjoyed for food. Yeonju liquor dipped in lotus leaf and yanghyang tea are spotlighted as their own liquor and tea. The fruit of lotus is lotus. Lotus meat is sweet and salty and its properties are flat. Lotus meat is found to be rich in protein, fat, carbohydrates, vitamins and minerals. It is good for people who are surprised, insomnia or nervous.

Longan meat used in the present invention (龍 眼 肉 에서 Euphoria longana) is a deciduous arborescent tree of the patient-free tree family, and writes the fake seed shell of the fruit. It is mainly used to return blood to the rain, to control forgetfulness and phobia, and to help wisdom. ' It is also called original and dragon.

The yellow lotus used in the present invention (黄連 scientific name Coptis chinensis ) is native to China and may be grown in Korea, Japan, China and the like for herbal medicine. It grows in mountain forests, eczema in the shade. Stems in the ground are thick and stretched to the side, with many beard roots, 4 to 5 root leaves at the end of stems, 10 to 27 cm long. The leaf is a double leaf with three small leaves. The small leaf is slightly hardened, sharp and serrated. Petioles become leaf house and sawtooth at leaf edge. A flower stem, about 10 cm in diameter, in March to April, blooms 2-3 white flowers. Flowers include bisexual and male flowers. White calyx pieces grow to 5-6 long and look like petals. Petals are 8 to 15 small, linear. There are many surgeries and 9 to 16 pistils. The ovate grows and the fruit is lined in a ring shape on it. Its name is given because the cross section of stem and underground stem is dark yellow. In oriental medicine, the roots of the 5-6 year old Hwangnyeon and Korean Waxy Lactobacillus are harvested and dried in the sun. It is prescribed for the treatment of vomiting, dysentery, heart fever, mental anxiety, sore throat, hemorrhage, nosebleeds, bleeding, and burns.

Doin (桃仁) used in the present invention, when the peach ripens around July-August, collects seeds and breaks out the hard seed shells [꺼내] to write out mainly the medicinal herb. It is an egg-shaped one-piece small lump, 1.2-2.0 cm long, 0.6-1.2 cm wide, 0.3-0.7 cm thick, wrapped in a reddish brown thin shell, and white inside. The taste is slightly bitter and water is added to break the fragrance of benzaldehyde. Used for medicinal anti-inflammatory gore blood (驅 瘀血 劑), etc., if the lower abdomen swelling, it is applied to menstrual irregularities, appendicitis (蟲 垂 炎). Herbal prescriptions that include this include Dokukseunggi-tang (桃核 承 氣 湯), Rhubarb Moranpitang (大黃 牡丹皮 湯), Kejijibokyeonghwan (桂枝 茯 笭 丸) and the like.

Safflower ( Chamhamus tinctorius ) used in the present invention is also called hongram (紅藍), safflower (이 花), dianthus, safflower. An annual plant of Asteraceae, its appearance is similar to thistle and about 1 m high. It is sown in early spring and blooms in July. The flowers bloom yellow and turn red. The leaves are alternate, broad lanceolate, and the serrated tip looks like thorns. Flowers bloom in July-August and look like thistles, but they are reddish yellow and hang on the ends of branches. The gun is wrapped in leafy caps and spines at the edges. Fruits are achene, 6 mm long, glossy, with short tube. Seeds are white. When wet in the early morning dew, picked and dried flowers are called safflower. Put safflower in water to dissolve the yellow pigment, wash it well in water and soak it in lye. The candle was put here and used as a rolled paper, and cloth and paper were dyed. It is also dyed with cloth wrapped in Egyptian mummies. The oil was squeezed with fruit to make kerosene and edible oil, and it was made from soot from lamp lamps. Seed oils contain a lot of linolic acid, which is good for the prevention and treatment of atherosclerosis caused by excess cholesterol. It is grown in Korea, India, China, Egypt, Southern Europe, North America and Australia. It has been called safflower for a long time in Korea, and the petal is widely used for women's disease prescription and women's medicine for pain and medicine. It is native to Egypt and is now grown in China, Japan, India, and the United States. Safflower seed is effective in reattaching broken or broken bones and has a unique effect on many bone diseases, such as osteoporosis and bone dysplasia.

Angelica gigas (當歸 scientific name) used in the present invention is a name that refers to the root of Seunggeumcho. Seunggeumcho is a perennial plant of the genus Minariaceae in the mountains, and is similar to the asshole. The stem comes out from the big root and grows about 1 m. The leaf is the upper right biplane. In summer, small white flowers bloom and the scent is unusual and strong. Angelica is a very powerful medicine with a blood donor function has been used a lot as a main ingredient from the old days. Angelica has a warm temper, heart to help the vulnerable, and has a blood drive to drive bad blood.當歸 means to go back to the place of course. In other words, when the body's blood donation is confused when taking the action to return the blood to its place. In addition, it has been reported that taking the Angelica extract and inhibited or destroyed the growth of cancer cells, it is also a herbal medicine that has been studied in recent years. Medicinal Angelica uses a slightly different plant from one, one, and one. The medicinal donkey commonly used in Korea is called "Gangdang-gu", and its scientific name is "Angelica gigas" and it is "2/3 year old herb". In Japan, the medicinal donkey is called "Wagging Angelica" and its scientific name is "Angelica acutilobae", and it is "perennial herb". In China, medicinal donkey is called "Chinese donkey" and its scientific name is "Angelica sinensis" and "perennial herb". These "donkeys" are slightly different in efficacy and weakness, but are used interchangeably for medicinal purposes. In addition, when medicinal donggi may be divided into "Dang ghost", "Dang gui" according to the part.

Paeonia suffruticosa Andrews., Used in the present invention, is native to western China and is believed to be a naturalized plant introduced in China due to the records of Queen Seondeok in the Silla period. It has strong cold resistance and is distributed throughout the country except North Hamgyong, but it is cultivated in Danyang-gun, Jewon-gun, and Hwasun-gun, Jeonnam. Perennial deciduous shrub, 1 ~ 1.5 m high. Leaves alternate, two-time exudates, egg-shaped or frenzied, and degenerative lobules are always divided into three, and lateral lobes are arranged in three rows. Flowers are single and large in size, with 5 sepals, 5 or more petals, and most of the cultivars have double flowers, many variations, and rose, red, purple, and white. There are several stamens, the operating table is red, pistil is 2 to 5, pistil is lobed, and the flower bed is cup-shaped. The outer surface is dark brown to dark red, and there are small oval side root marks on the side, and there are vertical wrinkles. The inner side is light brown to dark red and flat. The fracture surface is rough and white to pink powdery. The unique smell makes it easy to identify and tastes a bit spicy and bitter. The white and paeonol are attached to the inner and curved sides, and the stronger the fresh scent, the better the product. A shrub with peonies, planted with flowers or herbs from various places. In the fall, digging roots, pluck the neck and dry in the sun. Root bark and cores include the pheonol glycosides phenosides, pheonolides, peonyflorin, benzoic acid, and pheonol. It is used for autonomic nervous system relief as an antipyretic, antipyretic, analgesic, and anti-inflammatory agent. It is also used for menstrual irregularities and back pain. When used together with the skin and peach seeds (doin) increases the anti-inflammatory effect and blood purification.

Killing (竹茹 Bambusae Caulis in taeniam) used in the present invention is to dry the intermediate layer removed the outer shell of the stem, such as cotton rods perennial evergreen wood belonging to the bamboo family (Bambusaceae). It can be harvested all year round, but the new bamboo of the year after felling in winter solstice is good. Take fresh stalks, remove the outer sheath, strip off the slightly greenish middle layer in the form of a long thread and dry in the shade. The medicinal herb is fine, green, soft and elastic. It is mainly used for coughing or sweating caused by fever.

Sanjoin, ganoderma lucidum, lotus root, longan meat, yellow lotus, cauliflower, safflower, donkey, bark skin, kill contained in the 'hwahwa extract' provided by the present invention is mixed and extracted in an amount of 1 to 20 parts by weight based on 100 parts by weight of the total If it is out of the above weight range, it is not preferable due to deterioration of product stability such as skin side effects and precipitation due to excessive use of the medicine.

The 'hwahwa extract' provided by the present invention can be extracted by a conventional extraction method, and the type and content of the extraction solvent can also be optionally selected according to the purpose. As a specific example, rinsed with dried ones, extracts dried zojoin, ganoderma lucidum, soft meat, flesh meat, yellow lotus, cauliflower, safflower, donkey, bark skin, kill with one or more solvents selected from the group consisting of lower alcohol having 1 to 4 carbon atoms and ethylene glycol, butylene glycol can do.

The cosmetic composition for preventing skin aging having improved blood flow, improved cell proliferation activity, and antioxidant activity according to the present invention is not particularly limited in the formulation, for example, lotion, essence, cream, pack, gel, lotion, ointment, patch Or in the form of a spray or the like.

In the formulation, the content of 'hwahwa extract' is preferably included in 0.0001 to 30% by weight, preferably 0.001 to 20% by weight relative to the total weight of the composition. In this case, when the content of the extract is less than 0.0001% by weight, the blood flow improvement or cell proliferation activity effect to be achieved in the present invention cannot be expected due to the lack of content, and when it exceeds 30% by weight, there is a risk of side effects and the increased content. As much as the effect is not expected.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by the following Examples.

[Extraction example]

First, completely dry Sanjo 30g, ganoderma lucidum 30g, lotus root 30g, flesh 30g, rhubarb 30g, cabbage 30g, safflower 30g, donkey 30g, bark skin 30g, kill 30g to 100-200 mesh size using a Chinese medicine grinder and then grind 100 g was added to 1 L of 85% ethanol, and extracted by chilling for 5 days.

The medicinal herb extracted with ethanol was used to remove solids using Whatman filter paper No. 4, and then concentrated under reduced pressure using a rotary evaporator to obtain a 'flower extract'.

Example 1

Measurement of skin blood flow improvement efficacy for human body

Skin blood flow improvement efficacy test was performed on the human body using the 'hwahwa extract' obtained by the extraction example of the present invention.

PeriFlux 5000 manufactured by Perimed AB, Sweden, was used to test the blood flow promoting effect on skin in 50 healthy men and women. System 5000) was measured using a blood flow measurement device. In this device, red blood cells flowing through capillaries convert laser light into wavelengths, and the converted wavelengths are captured by returning fibers and converted back into electrical signals to express the blood flow by expressing these electrical signals numerically.

That is, the higher the blood flow, the higher the value, and the smaller the blood flow, the lower the value. Values representing blood flow are expressed in LDPF units provided by the device itself. The test subject was acclimated to a laboratory maintained at a constant temperature and humidity for 20 to 30 minutes, and then measured blood flow before applying the sample to the forearm, which is a test site, and waited 10 minutes after applying the sample, and then measured the blood flow again. It was.

As a control sample, 'flower extract' using distilled water as a solvent was used. The degree of blood flow improvement was calculated by expressing the ratio of blood flow measurement values before and after sample application as a percentage, and the results are shown in Table 1 and FIG. 1.

'Saturated extract' concentration (% by volume, solvent distilled water) Blood flow measurement average (LDPM unit) % Improvement A B Example 1 1.0 5.4 ± 1.2 6.9 ± 1.5 27.8 Example 2 5.0 5.8 ± 1.9 8.3 ± 1.4 43.1 Control group 1 - 5.3 ± 1.5 5.2 ± 1.1 -

※ % Improvement = (BA) / A × 100

A: Average value of blood flow before treatment of 'flower extract'

B: Blood flow average value after treatment of 'flower extract'

As shown in Table 1 and FIG. 1, 5.0% (v / v) of 'flower extract' showed an excellent blood flow improvement effect of 43.1%.

Example 2

Efficacy of cell proliferation activity

In order to measure the effect on the cell proliferation activity and toxicity of the hwahwa extract obtained by the extraction example of the present invention by culturing human fibroblasts MTT reduction method [3 (4,5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide reduction method] was performed and the formazan product was extracted with isopropyl alcohol, and the absorbance at 570 nm was measured and compared.

As an experimental control, a culture solution (a medium containing 1% FBS) without using a sample was used. By using the following equation 1 to calculate the proliferation capacity of the cells as shown in Table 2 and FIG.

Flower extract concentration (㎍ / ㎖) Cell proliferation (%) Example 3  0.1 105.4 Example 4  One 106.2 Example 5  10 110.7 Example 6  100 121.8 Example 7  1000 135.6 Example 8  10000 142.3 Example 9  100000 155.9

  * Number of repeat tests = 5

As shown in Table 2 and Figure 2, the 'flower extract' was confirmed that the cell proliferation is active up to 155.9% from the minimum treatment concentration compared to the control.

Antioxidant Effect Measurement

In order to examine the antioxidant effect of the 'hwahwa extract' obtained according to the preparation example of the present invention was carried out a free radical scavenging activity test (Free Radical Scavenging Activity Test). The free radical scavenging activity was modified from the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303 (1993)), and the stable free radical DPPH (1,1-diphenyl-2- picryhydrazyl, Sigma) reagent was used. 1 ml of 0.2 mM DPPH solution (methanol in the case of Blanc) was prepared at various concentrations of the 'flower extract' obtained in the above extraction example, and each of them was added and mixed with 2 ml, and the mixture was left at room temperature for 10 minutes. An experimental group for measuring absorbance at nm was set, and the control group was set to the case of using purified water. After absorbance measurement for the experimental group and the control group, the free radical scavenging activity was obtained using Equation 2 below, and the results are shown in Table 3 and FIG. 3.

matter density(%) Free radical scavenging rate (%) IC ₅₀ Flower extract 0.001 0.7 0.1% 0.005 4.6 0.01 4.8 0.05 24.2 0.1 41.4 0.1 81.2 One 85.2 5 85.4

     * Number of replicates = 5

In Table 3 and FIG. 3, IC ₅₀ is a concentration of a sample required to achieve 50% free radical scavenging ratio, which is a value used in comparison with other component substances, and the smaller the value, the higher the scavenging ratio. . Usually, Peony extract has 15.51% of IC ₅₀ , 4.21% of Sewage extract and 14.40% of Golden Root extract. As can be seen from the results shown in Table 3, the 'hwahwa extract' was confirmed that the free radical scavenging ability is relatively excellent compared to other component materials.

[Production Example 1]

The 'hwahwa extract' obtained from the extraction example according to the present invention was added as an active ingredient, respectively, to prepare a cream with the ingredients and contents shown in Table 4 below.

Comparative Example 1

Cream was prepared with the ingredients and contents shown in Table 4 without adding 'hwahwa extract'.

Ingredient (% by weight) Preparation Example 1 Comparative Example 1 Flower extract 10 - Stearic acid 3 3 ethanol 3 3 Potassium hydroxide 2 2 glycerin 10 10 Propylene glycol 2 2 antiseptic Quantity Quantity Pigment Quantity Quantity incense Quantity Quantity Purified water to 100 to 100

[Production Example 2]

The purified extract obtained from the extraction example according to the present invention was added as an active ingredient, and the flexible longevity was prepared with the ingredients and contents shown in Table 5 below.

Comparative Example 2

Without adding the extract, softening water was prepared in the ingredients and contents shown in Table 5.

Ingredient (wt%) Preparation Example 2 Comparative Example 2 Flower extract 5 - ethanol 3 3 Polylauric acid polyoxyethylene sorbitan 2 2 Methyl paraoxybenzoate One One glycerin 10 10 1,3-butylene glycol 2 2 incense Quantity Quantity antiseptic Quantity Quantity Pigment Quantity Quantity Purified water to 100 to 100

[Production Example 3]

The pack was prepared by containing the extract extracted from the extraction example of the present invention as an active ingredient and the ingredients and contents shown in Table 6 below.

Comparative Example 3

A pack was prepared with the ingredients and contents shown in Table 6 below without adding the extract.

Ingredient (% by weight) Preparation Example 3 Comparative Example 3 Flower extract 10 - glycerin 10 10 Propylene glycol 5 5 Polyvinyl alcohol 3 3 ethanol 2 2 Polyoxymethylene oleylethyl 0.5 0.5 Methyl paraoxybenzoate 1.5 1.5 incense Quantity Quantity Pigment Quantity Quantity antiseptic Quantity Quantity Purified water to 100 to 100

[Production Example 4]

The extract obtained from the extraction example according to the present invention was added as an active ingredient and an essence was prepared with the ingredients and contents shown in Table 7 below.

[Comparative Example 4]

Essence was prepared in the ingredients and contents shown in Table 7 without adding the extract.

Ingredient (% by weight) Preparation Example 4 Comparative Example 4 Flower extract 10 - Propylene glycol 5 5 glycerin 10 10 Hyaluronic acid 5 5 Polyoxyethylene Cured Castor Oil 6 6 Methyl paraoxybenzoate 1.5 1.5 ethanol 3 3 incense Quantity Quantity Pigment Quantity Quantity Purified water to 100 to 100

The method used to verify the blood flow improvement, cell proliferation activity efficacy, antioxidant effect by the cream, supple cosmetics, pack, essence prepared as in Preparation Examples 1 to 4 are as follows.

50 healthy men and women were selected to apply the creams, supple cosmetics, packs, and essences prepared in Preparation Examples 1 to 4 to the forearm portions of both arms in a size of 3 cm in diameter, and then improve blood flow by the methods of Examples 1 to 3. Was measured.

According to the measurement results, the cosmetics containing the flower extract prepared according to Preparation Examples 1 to 4 as an active ingredient showed an improvement in blood flow in at least 40 of 50 test subjects, and no side effects appeared on the skin. Did.

Verification of the safety of the 'flower extract' according to the present invention was performed by the following method.

Cytotoxicity Experiment

Method of MTT test by culturing V 79-4 cells (Chinese hamster, continuous cell line of lung tissue fibroblasts) on 'flower extract' to verify primary safety as raw material used for external skin preparations [Ref .: Mossman T. Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays. Journal of Immunological Methods , 1983, 65 , 55 ~ 63] shows the results of cytotoxicity test in Table 8.

sample ^a IC ₅₀ (%, w / v) SLS 0.005 Flower extract 5

* ^a IC ₅₀ : Inhibitory Concentration 50, concentration that kills 50% of cells

As shown in Table 8, the IC ₅₀ value of the 'flower extract' was 10%, indicating that cytotoxicity was 2000 times or more lower than that of the SLS, indicating excellent safety.

Allergy Assessment (LLNA)

As an experiment to confirm the allergens of the 'flower extract', the local lymph node assay (LLNA) was selected. [Reference: OECD GUIDELINE FOR THE TESTING OF CHEMICALS; DRAFT NEW GUIDELINE 429: Skin Sensitization; Local Lymph Node Assay. 'Hwa extract' is prepared as a 20%, 40%, 60% solution, 25 μl each of the mouse (Balb / c) ears are applied for 3 days, and then separated from the mouse auricular lymph node (Auricular lymph node). The isolated lymph nodes were pulverized into single cells, incubated for 24 hours with the addition of radioisotope [ ³ H] -methylthymidine, and then measured for amplification [dpm] of cell proliferation. The results are shown in Table 9.

sample density(%) Lymph node count (cells / mL 107) ^a dpm (average) ^b SI (amplitude) ethanol 60 1.3 2931 Transportation Flower extract 20 1.27 3914 1.3 Flower extract 40 1.14 2230 0.8 Flower extract 60 1.42 2289 0.8

^a dpm (disintegration per minute):

The amount of [ ³ H] -methyl thymidine inserted into the cell

^b SI (stimulation index):

   Average dpm of sample divided by average dpm of control

As shown in Table 9, the allergy evaluation result indicates the lymphocyte amplification degree of the sample group compared to the control group, and the amplification degree (SI: stimulation index) of the sample is higher than 3 even at one concentration, and allergen is shown to increase in a concentration-dependent manner. The extracts were found to have an amplification degree (SI) of 3 or less and were not allergenic.

From the above experiments, 'hwahwa extract' is a substance with excellent blood flow improvement effect, cell activity proliferation effect and antioxidant effect, and also excellent in toxicity and safety for the skin, lotion, essence, lotion, cream, pack, gel, ointment, patch, Or in the form of a spray or the like.

Human patch test

In order to find out the safety of the 'hwahwa extract' for the human body, a primary stimulation test was performed on the human body through a human patch test as follows. CTFA guidelines for 50 healthy men and women [Ref. The Cosmetic, Toility and Fragrance Association. Inc. Washington, D.C., 20036, 1991]. 15 μl of the sample and the solution were added dropwise to the Finn Chamber, which was placed on the back of the human body, which was the test site, and fixed with tape. Subsequently, after patching for 24 hours, after removing the patch and passing 4 hours again, the skin reaction of the test site was determined according to the following criteria, and the results are shown in Table 10.

<Judge criteria>

0 ; No erythema or unusual symptoms

One ; Slightly redder than the surroundings

2 ; Significantly redder than the surroundings

3; Severe redness and swelling than around

Stimulus level = [{0 × name} + {1 × name} + {2 × name} + {3 × name}] / number of subjects

Judgment result (person) Stimulus 3 2 One 0 Distilled water - 3 8 39 0.28 SLS (0.05% (w / v)) One 5 14 30 0.54 Flower extract (5% (v / v)) - - 3 47 0.06

As shown in Table 10, 'hwahwa extract' 5.0% (v / v) is determined to be a safe raw material without stimulation during application.

The cosmetic composition containing the extract as an active ingredient according to the present invention as described above has an excellent advantage of preventing aging of the skin due to improved blood flow, cell activity and antioxidant efficacy. In addition, since the extract is a natural substance, it can be safely used as a cosmetic without side effects on the skin.

The present invention described above is not limited to the detailed description and examples of the above-described invention, and various modifications are made by those skilled in the art without departing from the spirit and scope of the present invention described in the claims below. And modifications are also included within the scope of the invention.

Claims (3)

In the cosmetic composition for preventing skin aging, Skin aging cosmetics containing herbal extracts ('flower extract') extracted by mixing Sanjoin, Ganoderma lucidum, lotus root, longan meat, rhubarb, cabbage, safflower, donkey, bark skin, and killer in an amount of 1 to 20 parts by weight, respectively. Composition. The method of claim 1, The content of the active ingredient consisting of the mixed extract of the sanjoin, ganoderma, lotus root, longan meat, rhubarb, cabbage, safflower, donkey, bark skin, kill is 0.0001 to 30% by weight relative to the total weight of the cosmetic, for preventing skin aging Cosmetic composition. The method of claim 1, The cosmetic composition formulation is a skin lotion, essence, lotion, cream, pack, gel, ointment, patch or spray, characterized in that the anti-aging cosmetic composition.

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