KR101480695B1 - Cosmetic composition containing Scutellaria baicalensis extract using processing of herbal medicine - Google Patents

Abstract

The present invention relates to a cosmetic composition containing golden extracts processed by using a forage, which is a herbal processing technology, and more particularly to a cosmetic composition containing (a) boiling, steaming, roasting, roasting, A step of processing gold through a fog such as a process in which these are mixed; And (b) an extract of FoGeGold produced by the step of obtaining a processed gold extract, thereby improving skin elasticity and exhibiting antioxidant activity.

Spores, spores, figures, golden, herbal medicine, herbal medicine, processing, anti-aging, elasticity, cosmetic

Description

[0001] Cosmetic composition containing Scutellaria baicalens extract using processing of herbal medicine [

The present invention relates to a cosmetic composition having an anti-aging effect and an elasticity-improving effect by containing a golden extract processed by using a forage, which is a herbal processing technique.

Foje (匍 制) is a pharmaceutical technology (pharmaceutical technology) that changes the original properties of medicines by processing the medicines based on the oriental theory. Some medicines are toxic or very strong in nature and can not be taken directly. Some medicines can not easily be stored for a long time and others can be used only after removing some parts and parts. Also, even if the same medicines are used, there is a case where the properties of the raw material and the raw material are not same or different from each other. Before using this medicinal product, it must be processed, which is called a medicinal plant.

Foamers are sometimes processed together by applying a supplement, which is to achieve the purpose of the drug according to the dialect. Because of the variety of types of pills used in drug formulations, they have different properties and actions, and the resulting action of the medicinal formulations is different. There are a myriad of commonly used types of supplements, but generally they can be broadly categorized as liquid supplements and solid supplements. Liquid ingredients include alcohol, vinegar, honey, ginger juice, licorice juice, black bean juice, saline, unmeaned water, milk, urine (urine of children under the age of 7) and lime water. Solid foods include rice, bran, Tofu, earth, ply, sand, etc. are used.

The purpose of purifying herbal medicine is to ① clean the drug and make it easy to store ② reduce or eliminate the toxicity and side effects of the drug ③ change the drug to make the drug more effective ④ improve the therapeutic effect of the drug And (5) to remove the bad smell and taste of the medicinal materials so as to make them good for taking.

Golden (黄 芩), one of the representative medicines used in the application of herbicidal method in oriental medicine, is an herbaceous plant belonging to Lamiaceae (Lamiaceae) and medicines mainly roots. The medicinal properties of the medicinal material are circumferential-semicircular or flat-like, 5-25 cm long, 5-30 mm in diameter, and outer surface is yellowish brown with coarse and distinct vertical wrinkles. Fragments remain. In old roots, the throat rotates to dark brown, sometimes hollow, and the vagina is hard and brittle. The typical efficacy of gold in one room is detoxification of the shed, detoxification of the fire, detoxification of the heat, cooling of the heat and drying of the moisture, and hemostasis.

In Oriental medicine, gold is used to remove the substance from living things and cut into a certain size. It is known that when used, it strengthens the action of cheongryeonhwa (淸 热 火火). In addition, golden candle (fried, processed by firepower) is used to stabilize the fetus (胎 气) by alleviating the cold nature inherent in gold. Liquor, which is widely used as a liquid medicine, has an ascending property, so the efficacy of the medicinal material is elevated to the upper part of the human body. It is used to cool the upper heat when golden is fermented. On the contrary, when golden is fried, it acts on the lower part of the human body (herbal medicine medicine, Kim Ki Young et al., Shin Il Sasa; herbal medicine formulations and clinical application, Choi Ho Young et al.

The elasticity of the skin is treated by the extracellular matrix (ECM) component of the dermis tissue. The ECM is composed of two components. One is elastic fiber, which accounts for about 2 to 4% of the total ECM, and the other is collagen, which accounts for about 70 to 80% of the total ECM. Elastic fibers have the form of microfibrils embedded in an amorphous matrix called elastin. Elastin is found in the elastic fibers of desmosine and isodesmosine derived from lysine. It is a protein composed of unique amino acids. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In addition, elastic fibers composed of elastin are present along with collagen fibers called collagen, and it is possible to maintain skin elasticity in the state where elastin and collagen are sufficiently present.

The lowering of the skin elasticity is caused by degradation or destruction of collagen and elastin caused by aging or ultraviolet rays. In particular, the expression of matrix metalloproteases such as collagenase and elastase degrades collagen and elastin normally produced in the skin, resulting in reduced skin elasticity.

Various materials have been developed and used for the purpose of inhibiting the reduction of collagen and elastin, which are the causes of the decrease in elasticity. Retinoids such as retinol and retinoic acid exhibit elasticity improving effects (Dermatology therapy, 1998, 16, 364), the protein fraction obtained from Leguminosae Seeds exhibits an elasticity-enhancing effect (U.S. Patent No. 5,322,839), a composition containing malt extract and the like is used for inhibiting collagenase (Japanese Patent No. 5,105,693). However, these retinoids have disadvantages that even a small amount of skin is applied to the skin, irritation occurs. Most of the raw materials derived from natural materials have been used in the form of simple extracts, and it is not known what kind of substances the efficacy of the extracts is due to It is difficult to continuously maintain and control the activity of the extract.

Accordingly, the inventors of the present invention have found that a gold extract that has been processed by a foaming method has an effect, and completed the present invention.

The inventors of the present invention have found that, as a result of research to find out a raw material having more excellent anti-aging and elasticity improvement effect, The anti-aging and the elasticity-improving effect are more excellent, and the present invention has been completed.

Accordingly, an object of the present invention is to provide a cosmetic composition having an anti-aging effect and an elasticity-enhancing effect by preparing a gold extract using a feces and containing the extract as an active ingredient.

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition containing a gold extract using a fogue.

In addition, the present invention provides a cosmetic composition for improving skin elasticity containing a golden extract using a fogue.

Also, the fogue may be prepared by a) frying in a method of boiling, steaming, roasting, firing, stirring, or mixing gold; And b) obtaining a fogue extract of gold through step a) using water or an organic solvent.

The extract of Foje Gold of the present invention is more excellent in inhibiting the expression of elastase and collagenase than the simple extract. In addition, skin elasticity enhancement through the synergistic effect of the two activities was also superior to the simple extract. Therefore, the above-mentioned extract can be effectively used as a component for providing excellent anti-aging and elasticity-improving effect by being contained in cosmetic composition

The golden extract used in the present invention is prepared by using forage, a herbal processing technique, and the cosmetic composition containing the extract as an active ingredient can provide anti-aging and elasticity-enhancing effects.

The extraction method of gold used in the present invention is as follows.

(1) Foam processing step.

It is also possible to perform fogging by boiling, steaming, frying, roasting, firing, or stirring the gold, or by mixing the above methods to perform fogging. In case of boiling, 0.1-1 hour at 100-180 ℃, 1 - 48 hours at 150-200 ℃ for steaming, 0.1-1 hour at 100-300 ℃ for roasting, 0.5 - 5 hours at 180-300 ℃ for roasting . ≪ / RTI >

(2) obtaining an extract.

Water or an organic solvent is used to obtain a fogic extract of the golden through the above step (1). The organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

The cosmetic composition of the present invention contains the extract of gold extracted by the above method as an active ingredient in an amount of 0.001-30.0% by weight based on the total weight of the composition.

Hereinafter, the present invention will be described in more detail with reference to the following examples and test examples. However, these examples and test examples are provided only to aid understanding of the present invention, and the scope of the present invention is not limited to these examples.

[Comparative Example 1] Production of gold product

To 1 kg of dried gold, 5 L of an 80% aqueous ethanol solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden extract.

[Example 1] Preparation of Golden Leaf

1kg of clean gold was sprinkled evenly over a certain amount of sulfur (10 ~ 30%) and immersed so as to become damp enough to absorb all the alcohol, and put in a suppository container and fried at 100 ~ 180 ℃ for 15 ~ 30 minutes. After taking out the golden frying powder, it was cooled, 5 L of 80% aqueous ethanol solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden almond extract.

[Example 2] Preparation of golden hibachi

1kg of dried gold and 150-300g of vinegar were sufficiently absorbed into the medicinal materials and roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and then dried in the shade. To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden horsetail extract.

[Example 3] Production of golden coals

1 kg of dried gold was carbonized on the outside at 230 to 300 ° C. When it became black, the heating was stopped. After cooling at room temperature, 5 liters of 80% aqueous ethanol solution was added and the mixture was refluxed three times and immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden supercarum extract.

[Example 4] Production of golden millet

1kg of dried gold, 200 ~ 300g of honey was absorbed enough into the medicinal material, then roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and then dried in the shade. To this was added 5 liters of an aqueous 80% ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden millet extract.

[Example 5] Preparation of golden salt (salt)

1kg of dried gold is mixed well with salt water (2 ~ 3%), and is left to be completely closed to be completely absorbed. The gold with completely absorbed salt water is put into a supergiant vessel at 100 ~ 180 ° C, roasted for 10 minutes to 1 hour, . To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden-base extract.

[Example 6] Preparation of golden fermented rice

Fresh ginger was crushed, and then double water was added to the mixture. The mixture was squeezed and squeezed. The ginger juice was prepared by repeating it 2-3 times. The ginger juice was sprayed with 100-150 g of ginger juice in 1 kg of gold. After the ginger juice had permeated, it was put into a container at 100-180 ° C., roasted for 10 minutes to 1 hour, and then dried in the shade. To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden fermented extract.

[Test Example 1] Measurement of inhibitory effect on elastase expression

The inhibitory effect of the golden and golden extracts obtained from Comparative Example 1 and Examples 1 to 6 on the elastase production was measured in comparison with tocopherol and EGCG.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing DMEM containing 2.5% fetal bovine serum (Dulbecco's Modified Eagle's Media) Lt; / RTI > until growth. Then, the cells were cultured in a serum-free medium for 24 hours. The gold and golden extracts of Comparative Example 1 and Examples 1 to 6 dissolved in serum-free medium, tocopherol and EGCG were treated for 24 hours at a concentration of 10 ^-4 mol , And cell culture fluid was collected.

The degree of production of elastase in the cell culture was measured using a commercially available elastase measuring device (Amersham Pharmacia, USA). First, the cell culture medium collected on a 96-well plate uniformly coated with primary ELASA antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. After 3 hours, the secondary elastin antibody bound to the chromophore was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, the color development inducing substance was added to induce color development at room temperature for 15 minutes, and 1 M sulfuric acid was added again to stop the color development reaction. The color of reaction solution became yellow and the degree of yellow was different according to the degree of reaction progress.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of expression of elastase was calculated by the following equation (1) Respectively. At this time, the reaction absorbance of the cell culture fluid collected from the non-treated group was used as a control.

compound Expression level (%) Untreated group 100 Tocopherol 89 EGCG 67 Comparative Example 1 90 Example 1 71 Example 2 72 Example 3 77 Example 4 82 Example 5 86 Example 6 79

As shown in Table 1 above, the degree of expression of elastase in Examples 1 to 6 in vitro was lower than that of the golden extracts of Comparative Example 1, and the elastase expression of the golden foxtail extract according to the present invention It can be confirmed that the inhibitory effect is better. In addition, compared with tocopherol and EGCG, which are known as elastase inhibitors, the inhibitory effect of the golden forage extract according to the present invention on elastase expression is superior to tocopherol and is similar to that of EGCG.

[Test Example 2] Collagenase (MMP-1)

The collagenase production inhibitory activity of the golden and golden extracts obtained from Comparative Example 1 and Examples 1 to 6 was measured in comparison with retinoic acid.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing 2.5% fetal calf serum-containing DMEM (Dulbecco's Modified Eagle's Media) And incubated at 37 ° C in an incubator with CO _{2 of} 5% until it grew to about 80%. Then, the gold and golden extracts of Comparative Example 1 and Examples 1 to 6 were treated for 24 hours at a concentration of 10 ^-4 mol, and the cell culture broth was collected.

The degree of collagenase production in the cell culture was measured using a commercially available collagenase assay device (Amersham Pharmacia, USA, Catalog #: RPN 2610). First, the cell culture solution collected on a 96-well plate uniformly coated with primary collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. After 3 hours, the second collagen antibody bound to the chromophore was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the color development substance (3,3 ', 5,5'-tetramethylbenzidine, sigma) was added and color development was induced at room temperature for 15 minutes. When 1 M sulfuric acid was added to stop the color development reaction, the color of the reaction solution became yellow And the degree of yellowing was different according to the progress of the reaction.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated by the following equation (2) Respectively. At this time, the reaction absorbance of the cell culture fluid collected from the non-treated group was used as a control.

compound Expression level (%) Untreated group 100 Retinoic acid 75 Comparative Example 1 93 Example 1 78 Example 2 80 Example 3 77 Example 4 82 Example 5 84 Example 6 79

As shown in Table 2, the collagenase expression levels of Examples 1 to 6 in vitro were lower than those of Gold Extract Comparative Example 1, and the collagenase expression of the golden forage extract of the present invention It can be confirmed that the inhibitory effect is better. In addition, in comparison with retinoic acid, which is known as a collagenase expression inhibitor, the collagenase inhibitory effect of the golden forage extract of the present invention is similar to that of retinoic acid.

From these results, it can be confirmed that the golden forage extract according to the present invention has an effect of inhibiting the substrate metalloprotease (MMP-1).

[Test Example 3] Confirmation of improving skin elasticity

In order to confirm the skin elasticity improving effect, the cosmetic lotions of Comparative Formulation Example 1 and Formulations 1 to 6 were prepared by using the golden and golden powder extracts obtained in Comparative Example 1 and Examples 1 to 6, Lotion without the golden forage extract was prepared as a control.

Component (content:% by weight) Control group Comparative Formulation Example 1 Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Cetearyl glucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl octanoate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Methylparaben 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 Disodium ethylenediaminetetraacetate 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Comparative Example 1 - 10 - - - - - - Example 1 - - 10 - - - - - Example 2 - - - 10 - - - - Example 3 - - - - 10 - - - Example 4 - - - - - 10 - - Example 5 - - - - - - 10 - Example 6 - - - - - - - 10 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

In order to compare skin elasticity improvement effects of the control group, Comparative Formulation Example 1 and Formulation Examples 1 to 6, 140 women in their 30s and 40s were divided into 7 groups each consisting of 20 individuals. The experimental method is as follows.

To each group, control lotions, Comparative Formulation Example 1, or Formulation Examples 1 to 6 were applied to each eye, and a predetermined amount of the lotion was applied once a day for 12 weeks. At this time, the lotion of the control group was applied to the left side of the eye of the subject, and the lotion of Comparative Formulation Example 1 or Formulations 1 to 6 was applied to the right side. After 12 weeks, skin elasticity of each group was measured and averaged using a cutometer (SEM474, Courage + Khazaka electronic GmbH, Germany) capable of measuring skin elasticity, and the results are shown in Table 4 .

compound Skin elasticity improvement (%) Control group 18 Comparative Formulation Example 1 39 Formulation Example 1 58 Formulation Example 2 46 Formulation Example 3 48 Formulation Example 4 47 Formulation Example 5 50 Formulation Example 6 55

The results of Table 4 show that when the lotion formulations of Formulations 1 to 6 containing the golden forage extract according to the present invention were used, the elasticity of the skin was three times higher than that of the control without the gold extract , And it can be seen that skin elasticity improvement is much better than the case of using the cosmetic lotion of Comparative Formulation Example 1 containing a simple extract of gold.

Therefore, it can be confirmed that the cosmetic composition containing the golden forage extract of the present invention is very effective for improving skin elasticity.

Claims (5)

delete A cosmetic composition for improving skin elasticity containing a golden extract using a fogue. 3. The method of claim 2, a) frying in a method of boiling, steaming, roasting, burning, boiling or mixing gold; And b) obtaining a fogue extract of gold through step a) using water or an organic solvent; Wherein the cosmetic composition is a cosmetic composition for enhancing skin elasticity. The cosmetic composition for improving skin elasticity according to claim 3, wherein the organic solvent is at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform. The cosmetic composition for enhancing skin elasticity according to claim 2, wherein the gold extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition.