KR101970503B1 - Composition containing Scutellaria baicalensis extract and vinegars for scalp or hair - Google Patents

Abstract

The present invention relates to a composition for scalp or hair containing a golden extract and a vinegar, and more particularly to a composition for scalp or hair containing a golden extract and vinegar as an active ingredient to promote hair growth and prevent and improve hair loss .

Description

Compositions for scalp or hair containing a golden extract and vinegar {Composition containing Scutellaria baicalensis extract and vinegars for scalp or hair}

The present invention relates to a composition for scalp or hair containing a golden extract and a vinegar, and more particularly to a composition for scalp or hair containing a golden extract and vinegar as an active ingredient to promote hair growth and prevent and improve hair loss .

The hair of the human body is about 100 to 150,000, and each hair repeats growth and degeneration through different cycles. The hair grows in the growing anagen, the catagen which slows the metabolism while maintaining the shape of the hair, and the catagen that slows down the papillary and the hair follicle is gradually contracted and the hair follicles are pushed upward, ). The cycle and lifespan of such hair may vary depending on various conditions such as nutritional status, history, genetics, constitution, hormone secretion or aging.

Hair loss is a problem that occurs through natural processes or is chemically facilitated by the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss involves a lack of hair regrowth leading to partial or complete baldness.

Recently, as more and more people with alopecia due to environmental pollution, stress, diet, fatigue, and inadequate eating habits, the number of men or women suffering from serious mental suffering is increasing.

Korean Patent Publication No. 2010-0050647

Accordingly, the inventors of the present invention have studied a composition capable of preventing hair loss and activating hair growth by activating the proliferation of scalp cells without side effects in the scalp. As a result, it has been found that when a golden extract is used together with vinegar, It was found that the use of the golden extract can promote the proliferation of the dermal papilla cells and the proliferation of the dermal dermal cells, thus completing the present invention.

Accordingly, it is an object of the present invention to provide a composition capable of effectively promoting hair growth and improving hair loss.

In order to achieve the above object, the present invention provides a composition for scalp or hair containing a golden extract and vinegar as an active ingredient.

The composition of the present invention provides an antioxidative effect by using a golden extract together with vinegar, while enhancing hair growth by activating the cell proliferation of the scalp, thereby reducing hair loss and promoting hair growth.

FIG. 1 is a graph showing the growth rate of dermal papilla cells in the case of treatment with a golden horsetail extract using a golden extract and vinegar.
FIG. 2 is a graph showing the effect of increasing potassium ion channel activity on cell viability when treated with a golden horseradish extract using a golden extract and vinegar.
FIG. 3 is a graph showing the antioxidative effect in the case of treatment of a golden horsetail extract using a golden extract and a vinegar.

In the present specification, the term " promoting hair growth " means not only promoting the generation of new hair but also allowing the existing hair to grow healthily, and the phenomenon in which the hair falls off the scalp and the hair becomes thin And suppression.

The composition for scalp or hair according to the present invention contains a golden extract and a vinegar as an active ingredient, and preferably contains a golden extract which has been processed by a blush method.

The golden ( Scutellaria baicalensis ) used in the present invention is herbaceous plant belonging to Lamiaceae (Lamiaceae) and is a medicinal herb that is frequently used for the purpose of detoxification by heat in medicine. It mainly medicines roots. When you take the roots of gold and peel off the roots, it is called gold because it is colored like golden gold. Gold treats frequent chills and chills, mainly used for symptoms caused by heat in the lungs.

Foje is a pharmaceutical technology that changes the nature of a medicinal substance by processing the medicinal substance based on the oriental medicine theory. ① It makes the medicines clean and makes them easy to store. ② The toxicity and side effects ③ to make the medicine more effective by changing the weakness, ④ to enhance the therapeutic effect of the drug, and ⑤ to remove the bad smell and taste of the medicinal material and to make it better to take.

Foams are sometimes processed together by adding a supplement, and the use of the supplement is to achieve the purpose of the drug according to the dialect. Because of the variety of types of pills used in drug formulations, they have different properties and actions, and the resulting action of the medicinal formulations is different. There are a myriad of commonly used types of supplements, but generally they can be broadly categorized as liquid supplements and solid supplements. Liquid ingredients include alcohol, vinegar, honey, ginger juice, licorice juice, black bean juice, saline, unmeaned water, milk, urine (urine of children under the age of 7) and lime water. Solid foods include rice, bran, Tofu, earth, ply, sand, etc. are used.

In the present invention, a method of grilling a medicinal material in a medicinal material by roasting a medicinal material with a certain amount of a supplement (various kinds of alcohol, salt, honey, ginger, etc.) is used. In particular, (醋))) method which is used for

The vinegar used in the present invention is not particularly limited, but vinegar fermented with grains or fruits can be used. Preferably, rice vinegar or barley vinegar as a grain vinegar, apple vinegar or lemon vinegar as a fruit vinegar can be used.

In addition, in the present invention, it is possible to process gold by a commonly used chewing gum method, preferably by adding 5 g of vinegar to 100 g of gold. The gold and vinegar are then mixed with 80 Fry at ~ 200 ℃ for 1 minute ~ 1 hour to process the gold.

The golden extract or golden horsetail extract used in the present invention is obtained from gold treated with gold or porcelain method using water, hot water or an organic solvent. The organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

When the extract of the present invention is used in combination with vinegar, the extract of the present invention contains 0.001 to 50% by weight of a golden extract and 0.001 to 10% by weight of vinegar, based on the total weight of the composition.

In addition, when the golden glaze extract extracted by the above method is used in the composition of the present invention, the glaucophyll extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition.

The composition of the present invention maximizes the efficacy of the raw material by using the gold extract with the vinegar or by using the gold extract which is processed by the gravure method in particular, thereby providing excellent efficacies such as strengthening the hair root, promoting blood circulation and antioxidation, Promoting effect of wool and prevention and improvement of hair loss can be shown.

The composition of the present invention can be administered by any method, and can be administered parenterally or orally. The composition according to the present invention can be prepared by a conventional method according to a formulation. The formulations are not particularly limited as long as they can exert the effects of the present invention. For example, the formulations may be any formulations of external agents such as solubilizing agents such as tonics, emulsions such as emulsions and creams, ointments, dispersions, gels, aerosols, . In addition, its product form can be used for hair care medicines, quasi-drugs or cosmetics for the purpose of preventing hair loss, hair growth, hair growth, wool and the like, for example, hair growth accelerators, hair growth agents, scalp treatments, , A shampoo, a rinse, a hair pack, a lotion, a conditioner, a hair oil, a mousse, a cream or the like, or a skin cleansing agent. In addition, the composition of the present invention may be used in the form of solid, solution, emulsion, dispersant, micelle, liposome, ointment, etc., and may be combined with an organic or inorganic carrier or excipient suitable for oral or parenteral application. The compositions of the present invention may be in the form of tablets, powders, pellets, capsules, suppositories, solutions, emulsions, suspensions, solutions, jellies and any other forms suitable for use, May be mixed with an acceptable carrier. Usable carriers include solid, semisolid or liquid glucose, lactose, gum arabic, gelatin, mannitol, starch paste, magnesium trisilicate, talc, corn starch, corn starch, corroid silica, potato starch, urea, Triglycerides, dextrans, of medium chain length, and other carriers suitable for use in the preparation of preparations. Adjuvants, stabilizers, thickeners, coloring agents and perfumes can also be used.

Hereinafter, the present invention will be described in more detail with reference to the following examples and test examples. However, these examples and test examples are provided only to aid understanding of the present invention, and the scope of the present invention is not limited to these examples.

[Reference Example 1] Preparation of gold extract

1 kg of dried gold was subjected to reflux extraction three times with hot water and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden extract.

[Reference Example 2] Preparation of rice vinegar

Brown rice was sprinkled with koji powder and fermented for more than 3 months. Then, rice vinegar was prepared by filtering it.

[Reference Example 3] Preparation of barley vinegar

Barley roots were sprinkled with koji powder, fermented for 3 months or more, and then filtered to prepare barley vinegar.

[Reference Example 4] Preparation of apple vinegar

The apples were cleaned and cut into appropriate sizes in a jar or disinfected glass bottle, and the mixture was sieved with yeast powder, fermented for 3 months or longer, and then filtered to prepare apple vinegar.

[Reference Example 5] Preparation of lemon vinegar

The lemon was cleaned and washed in a suitable size and placed in a jar or disinfected glass bottle. The mixture was sprayed with a mixture of yeast powder and fermented for 3 months or longer, and then the mixture was filtered to prepare apple vinegar.

[Example 1] Preparation of golden horseradish extract using rice vinegar

To 1 kg of dried gold, 50-100 g of the rice vinegar of Reference Example 2 was sufficiently absorbed into the medicinal material and roasted at 100-160 ° C for 10 minutes to 1 hour and then dried in the shade. This was subjected to reflux extraction three times with hot water and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden horsetail extract.

[Example 2] Preparation of golden horseradish extract using barley vinegar

To 1 kg of dried gold, 50-100 g of the barley vinegar of Reference Example 3 was sufficiently absorbed into the medicinal material and roasted at 100-160 ° C for 10 minutes to 1 hour, followed by drying in the shade. This was subjected to reflux extraction three times with hot water and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden horsetail extract.

[Example 3] Preparation of golden horseradish extract using apple vinegar

To 1 kg of dried gold, 50-100 g of the apple vinegar of Reference Example 4 was sufficiently absorbed into the medicinal material and roasted at 100-160 ° C for 10 minutes to 1 hour, followed by drying in the shade. This was subjected to reflux extraction three times with hot water and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden horsetail extract.

[Example 4] Preparation of golden horseradish extract using lemon vinegar

To 1 kg of dried gold, 50-100 g of the lemon vinegar of Reference Example 5 was sufficiently absorbed into the medicinal material and then roasted at 100-160 ° C for 10 minutes to 1 hour and then dried in the shade. This was subjected to reflux extraction three times with hot water and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a golden horsetail extract.

[Test Example 1] Test for proliferation of dermal papilla cells

The keratin proteins that make up the hair are produced in hair follicle keratinocyte, which keratinocytes differentiate from the dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the present composition, DP6 (rat immortalized dermal papilla cell) cell line was used in the present invention (Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). This dermal papilla cell line is a cell line isolated by microdissection method in the hair roots of male PVG rat beard and is a cell line obtained from Dulbecco's modified Eagle's medium (Gibco BRL, Gaithersburg, MD, USA) containing 10% fetal bovine serum (FBS) USA) for 24 hours in an incubator maintained at 37 ° C in 5% CO ₂ . DP6 was cultured in a 96-well plate for 24 hours in a 37 DEG C incubator, and treated with the sample as described in the following Table 1, at a ratio of 20: 1 to the golden extract and vinegar. For comparison, only the solvent was treated in the control group and water was used as the solvent. After 24 hours of treatment, the cell proliferative capacity was measured using a WST-1 kit (Roche). The results are shown in Table 1 and FIG.

sample Cell proliferation (%) Untreated group 100 Solvent treatment group 500 ppm 102 Golden extract 1ppm 118 Rice vinegar 1ppm 110 Golden extract + rice vinegar 1ppm 122 Golden horseradish extract (rice vinegar) 1ppm 149 Golden horseradish extract (rice vinegar) 2ppm 147 Golden horseradish extract (rice vinegar) 5ppm 171 Barley vinegar 1ppm 109 Golden extract + barley vinegar 1ppm 120 Golden horseradish extract (barley vinegar) 1ppm 136 Apple vinegar 1ppm 107 Golden extract + apple vinegar 1ppm 118 Golden horseradish extract (apple vinegar) 1ppm 133 Lemon vinegar 1ppm 104 Golden extract + lemon vinegar 1ppm 118 Golden horseradish extract (lemon vinegar) 1ppm 135

As shown in Table 1, even when treated with the golden extract, the papilla cell proliferative capacity was higher than that of the untreated group. When vinegar was treated, the papilla cell proliferative capacity was less than that of the golden extract. However, in case of using a mixture of golden extract and vinegar, synergistic effect showed better dermal papilla cell proliferation ability.

In addition, it can be confirmed that the golden horsetail extract prepared by using vinegar exhibits about twice as much dermal papilla cell proliferative capacity as the case of mixing gold extract and vinegar at the same concentration.

[Test Example 2] Evaluation of Potassium Ion Channel Activity Increase Effect

In order to examine the effect of the substance used in the present invention on the activity of potassium ion channel, to inhibit cell proliferation by treatment with tolbutamide (SIGMA AlDRICH, T0891) blocking K _ATP channel in fibroblasts constituting the dermis of the scalp, And the cell proliferation was restored by opening the potassium ion channel again.

To evaluate the function of this composition as a K _ATP channel opener, the fibroblast cell line NIH3T3 (mouse embryonic fibroblast cell line) cell line was used in the present invention. This cell line is a cell line obtained by natural immortalization of fibroblasts isolated from NIH Swiss mouse embryo by the 3T3 protocol. The cell line was cultured in DMEM (Dulbecco's modified Eagle's medium; Gibco BRL, Gaithersburg, MD, USA) containing 10% bovine serum (FBS) for 24 hours in an incubator maintained at 37 ° C in 5% CO ₂ . NIH3T3 was cultured in a 96-well plate for 24 hours in a 37 DEG C incubator, treated with 2.5 mM tolbutamide, and treated 10 minutes later, as shown in Table 2, where the golden extract and vinegar were 20: 1 Respectively. As a positive control group, minoxidil, which is known as a potential mitochondrial potassium ion channel opener (K _ATP channel opener) and used for the treatment of androgenetic alopecia, was used for comparison. After 48 hours from the treatment of the sample, the cell survival rate was measured using a WST-1 kit (Roche). The results are shown in Table 2 and FIG.

sample Cell survival rate (%) Untreated group 100.0 Tolbutamide 2.5mM 58.8 Minoxidil 10 μM 131.1 Golden extract 50ppm 105.5 Rice vinegar 50ppm 102.7 Golden extract + rice vinegar 50ppm 107.2 Golden horseradish extract (rice vinegar) 50ppm 129.1 Barley vinegar 50ppm 101.9 Golden extract + barley vinegar 50ppm 106.8 Golden horseradish extract (barley vinegar) 50ppm 125.1 Apple vinegar 50ppm 101.4 Golden extract + apple vinegar 50ppm 106.1 Golden horseradish extract (apple vinegar) 50ppm 123.7 Lemon vinegar 50ppm 100.2 Golden extract + lemon vinegar 50ppm 106.3 Golden horseradish extract (lemon vinegar) 50ppm 123.4

As shown in Table 2, even when treated with the gold extract alone, the cell viability was higher than that of the untreated group, but the level of opening of the potassium ion channel was insignificant. The cell survival rate of gold extracts treated with vinegar was higher than that of gold extract alone.

In addition, in the case of the golden horseradish extract, the potassium channel was opened at a higher level than the case of mixing the golden extract and the vinegar at the same concentration, which was almost similar to that of the existing hair loss treatment, minoxidil.

[Test Example 3] Evaluation of antioxidative effect

DPPH antioxidant assay was used to evaluate the antioxidant effect of this composition. Oxidative reactions occurring in the body are mainly caused by free radicals, and this action is known to cause cell senescence and cancer. Therefore, inhibition of free radical formation in the body can be related to antioxidant and anti-aging in the body. Since this antioxidative effect is difficult to test for free radicals in the body, DPPH (2,2-diphenyl-1-picrylhydrazyl) It is universally used. The DPPH radical has a deep purple color. When it reacts with the antioxidant, it turns into a pale yellow, and its antioxidant ability is evaluated by measuring the absorbance at 517 nm wavelength. The higher the antioxidant capacity, the less yellowish the color and the lower the absorbance.

In this experiment, a 0.1 mM DPPH solution was added to a 96-well plate, and the sample was treated as described in Table 3 below. The gold extract and vinegar were used at a ratio of 20: 1. Vitamin C, a known antioxidant, was used as a positive control for comparison. After the sample was treated, it was reacted in an incubator at 37 ° C for 30 minutes and the absorbance was measured at 517 nm. The results are shown in Table 3 and FIG.

O.D. (517 nm) Untreated group 0.494 Vitamin C (positive control) 25ug / ml 0.218 50 ug / ml 0.143 100ug / ml 0.153 Golden extract 125ppm 0.421 Rice vinegar 125ppm 0.407 Golden extract + rice vinegar 125ppm 0.389 Golden horseradish extract (rice vinegar) 125ppm 0.309 250ppm 0.156 500ppm 0.100 Barley vinegar 125ppm 0.405 Golden extract + barley vinegar 125ppm 0.391 Golden horseradish extract (barley vinegar) 125ppm 0.312 Apple vinegar 125ppm 0.399 Golden extract + apple vinegar 125ppm 0.392 Golden horseradish extract (apple vinegar) 125ppm 0.311 Lemon vinegar 125ppm 0.402 Golden extract + lemon vinegar 125ppm 0.392 Golden horseradish extract (lemon vinegar) 125ppm 0.315

The results are shown in Table 3, which shows that the antioxidant ability of the extract alone was higher than that of the untreated group, but the level was insignificant. However, antioxidant activity of gold extract was higher than that of gold extract alone.

In addition, in the case of the golden horseradish extract, the antioxidative effect was much higher than that of the golden extract and vinegar at the same concentration. When 500 ppm of vinegar was used, the antioxidative effect was similar to that of the vitamin C Respectively.

The composition of the present invention can be formulated into a usable product through various routes as described below, but is not limited thereto.

[Formulation Example 1] Shampoo

The shampoo composition can be prepared in a conventional manner according to the composition shown in Table 4 below.

ingredient Content (% by weight) Golden Horseradish Extract 5 Sodium lauryl sulfate (30%) 10 Sodium polyoxyethylene lauryl sulfate (30%) 30 Polyquaternium-10 0.5 Dimethicone 2 Paraoxybenzoic acid ester 0.2 Spices 0.4 Sodium chloride Suitable amount Citric acid Suitable amount water To 100

[Formulation Example 2] ointment preparation

The ointment compositions can be prepared in a conventional manner according to the composition of Table 5 below.

ingredient Content (g) Golden Horseradish Extract 1.0 Dexpanthenol 1.5 Stearic acid 1.0 Liquid paraffin 5.0 Nonsense 4.0 Cetanol 3.0 Propylene glycol 13.0 Triethanolamine 1.5 Dibutylhydroxytoluene 0.025 Benzoic acid profile 0.015 Polysorbate 800.1 Purified water 63.0

Claims (10)

A composition for external application for skin for improving the condition of at least one scalp and hair selected from the group consisting of hair loss prevention, hair growth stimulation, hair growth stimulation, hair follicle stimulation,
Wherein the golden glaze is obtained by putting golden vinegar in a vinegar to absorb vinegar, roasting it at a temperature of 80 to 200 DEG C for 1 minute to 1 hour, and drying it on a shade to improve the condition of scalp and hair. Composition. delete [2] The method of claim 1, wherein the golden glaze extract is prepared by dissolving vinegar in vinegar, firing it at a temperature of 80 to 200 [deg.] C for 1 minute to 1 hour, Wherein the composition is obtained by reflux extraction with a mixed solvent of an organic solvent and water, followed by immersion. 4. The composition for external application for skin of scalp and hair according to claim 3, wherein the organic solvent is at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform. [2] The method of claim 1, wherein the golden glaze extract is prepared by dissolving vinegar in vinegar and then frying the mixture at a temperature of 100 to 160 DEG C for 10 minutes to 1 hour, drying the mixture on a shade, extracting the mixture with hot water, Wherein the external preparation for scalp and hair is a composition for improving the condition of scalp and hair. [Claim 2] The vinegar according to claim 1, wherein the vinegar is a vinegar obtained by fermenting rice or barley with nuruk for 3 months, or a fruit vinegar obtained by fermenting for 3 months after addition of malt to apple or lemon , Scalp and hair. [4] The vinegar according to claim 3, wherein the vinegar is a vinegar obtained by fermenting rice or barley with nuruk for 3 months, or a fruit vinegar obtained by fermenting for 3 months after addition of nuruk to apple or lemon , Scalp and hair. [Claim 5] The vinegar according to claim 5, wherein the vinegar is a vinegar obtained by fermenting rice or barley with nuruk for 3 months, or a fruit vinegar obtained by fermenting for 3 months after adding nourishing to apple or lemon , Scalp and hair. The composition for external application for skin of scalp and hair according to claim 1, wherein the golden horsetail extract is present in an amount of 0.001 to 50% by weight based on the total weight of the composition. delete

Images