KR102153414B1 - Composition for improving hair loss and promoting hair growth containing a fermented soybean - Google Patents

Abstract

The present invention relates to a cosmetic composition for reducing hair loss and promoting hair growth containing a fermented black soybean, a food composition, a feed composition, and a pharmaceutical composition for preventing or treating hair loss. The cosmetic composition contains fermented black soybean fermented with Korean infantile lactic acid bacteria, thereby reducing hair loss or promoting hair growth.

Description

Composition for improving hair loss and promoting hair growth containing a fermented soybean}

It relates to a cosmetic composition for improving hair loss and promoting hair growth, comprising a fermented soybean fermented product, a food composition, a feed composition, and a pharmaceutical composition for preventing or treating hair loss.

Drugs that have been approved by the US FDA as treatment for hair loss include minoxidil and Finasteride, but Propecia has typical side effects such as erectile dysfunction and decreased libido due to the mechanism that suppresses male hormones, and cannot be used for women of childbearing age. There is this. In addition, it is known that the minoxidil formulation Rogaine may cause side effects such as erythema, palpitations, and arrhythmia when the period of use is prolonged. In fact, minoxidil's hair loss treatment effect is very low at about 30% of the applied patients. Therefore, researches on raw materials for effectively treating hair loss diseases without skin irritation and hormonal side effects are being conducted in a variety of ways, and in particular, studies on functional cosmetics for improving hair loss of various natural substances have been conducted that have similar or higher efficacy as existing hair loss treatments. It is being done actively.

Among natural materials, soybeans contain about 18% fat, most of which are unsaturated fatty acids, and more than half of them are linolenic acid (Kim YH, Hawrylewiez EJ et al., 1992, Klein BP et al. 1995, Sirtori CR et al. 1995). In addition, it contains a large amount of functional ingredients such as isoflavones, soybean saponins, and oligosaccharides. (Kennedy AR 1995) In particular, among the various types of soybeans, rat-null-bean (Seori-tae) has been called Yak-bean because of its excellent pharmacological efficacy from ancient times, and is introduced as a medicinal with excellent detoxification effect in the herbal medicine book, Herbal Medicine. Yak bean contains 40% of essential amino acids, and antioxidants such as vitamin E, carotenoids, saponins, and anthocyanins are contained four times more than ordinary beans. According to recent studies, it has been reported that it protects blood circulation and liver function, as well as prevents senile dementia and improves kidney function. (Kim MH et al. 2008) Furthermore, there are various results of previous studies that can help skin health such as antioxidant, wrinkle improvement, moisturizing, and inflammation relief. It has been commercialized through the industry and is used as a health functional food and cosmetic composition.

Under this background, the present inventors have made efforts to maximize the efficacy of Yak Soy . As a result, it was confirmed that the effect of improving hair loss and promoting hair growth is remarkably increased when the Yak Soy is fermented using lactic acid bacteria ( Bifidobacterium animalis subsp. Thus, the present invention was completed.

In one aspect, Bifidobacterium animalis subsp. Lactis. LDTM 8102 (KCTC13392BP) strain was inoculated and fermented in Yak bean, containing fermented soybean as an active ingredient to improve hair loss or promote hair growth It is to provide a cosmetic composition for.

Another aspect is to provide a food composition for improving hair loss or promoting hair growth, containing fermented soybeans as an active ingredient, obtained by inoculating and fermenting the Yak Soybean with a strain of Vitidobacterium animalis subspecis lactis LDTM 8102 (KCTC13392BP).

Another aspect is to provide a feed composition for improving hair loss or promoting hair growth containing fermented soybeans as an active ingredient, obtained by inoculating and fermenting Yak bean with Vitidobacterium animalis subspecis lactis LDTM 8102 (KCTC13392BP) strain. .

Another aspect is to provide a pharmaceutical composition for preventing or treating hair loss containing a fermented soybean fermented product as an active ingredient, obtained by inoculating and fermenting a strain of Vitidobacterium animalis subspesis lactis LDTM 8102 (KCTC13392BP) in Yak Soybean.

In one aspect, Bifidobacterium animalis subsp. Lactis. LDTM 8102 (KCTC13392BP) strain was inoculated and fermented in Yak Soybeans, containing fermented soybeans as an active ingredient to improve hair loss or promote hair growth It provides a cosmetic composition for.

In this specification, the term “Yakkong” refers to small black beans with a black skin and smaller than ordinary black beans, and there are bark beans, Socheongja, Yakseon beans, Dawon beans, Socheong No. 2, etc., which are much smaller than ordinary black beans, but 　iso The flavone series of useful ingredients, genistein and daidzein, are contained far more than other black beans.

The yak bean may be pre-treated before fermentation. Specifically, the yak bean may be pretreated by performing roasting or grinding. In one embodiment, when the pretreatment is roasting, the roasting may be performed for 5 to 30 minutes at a temperature condition of 100 to 300°C. Specifically, the roasting is 100 to 300°C, 100 to 250°C, 100 to 200°C, 100 to 150°C, 150 to 300°C, 150 to 250°C, 150 to 200°C, 200 to 300°C, 160 to 300°C Alternatively, it may be performed under conditions of 160 to 250°C. In addition, the roasting may be performed for 5 to 30 minutes, 5 to 25 minutes, 5 to 20 minutes, 5 to 15 minutes, 5 to 10 minutes, 7 to 30 minutes, 7 to 20 minutes, or 10 to 30 minutes. have. At this time, if the roasting temperature is less than the above range, there is a problem that the flavor of the yak bean is not maintained, and the hair loss improvement or hair growth promoting effect is lowered, and if it exceeds the above range, the bitter taste of the yak bean becomes strong, and consumer preference decreases. There is a problem. In addition, when the roasting time is less than the above range, there is a problem in that the stability of taste is not maintained when applied as a food composition, and when it exceeds the above range, there is a problem that the weak beans are carbonized.

As used herein, the term " Bifidobacterium animalis subsp. Lactis.) LDTM 8102 (KCTC13392BP) strain" is isolated from feces of Korean infants and young children, and beta-glucosidase (β- glucosidase) is excellent in production capacity, so it can be effectively used for biotransformation into useful substances through microbial metabolism of biomass natural products including rice, barley, milk, soybeans, ginseng, seaweeds, woody, etc.

As used herein, the term "fermented product" means that raw materials are denatured or transformed in a physical or chemical way using lactic acid bacteria, and in nature, the desired result can be changed in tens of thousands of years. It refers to the decomposition process that can be obtained. Specifically, the fermentation product is a fermentation product produced by mixing Yak bean into a lactic acid bacteria culture medium, inoculating and fermenting lactic acid bacteria, and the lactic acid bacteria are Bifidobacterium animalis subsp. Lactis. LDTM 8102 (KCTC13392BP) may be a strain.

The fermentation product may refer not only to a product fermented by the lactic acid bacteria, but may include a fermentation filtrate or a fermentation extract obtained by extracting the fermented product from the fermented soybean as a solvent.

In the present specification, the term "fermentation extract" may refer to a useful substance itself generated by extracting the fermentation product, and more specifically, the fermentation strain and the fermentation product are removed by centrifugation from the fermentation strain culture medium coexisting A fermented product, a fermented product obtained by filtering a fermentation strain from the culture medium, a fermented product obtained by sterilizing a fermentation strain from the culture medium and filtering it, a dilution solution obtained by diluting the fermentation product, and a dried product obtained by drying the fermentation product. It may contain all of the materials. In the present specification, the term fermented extract may be used interchangeably with the fermented soybean extract.

The extraction may be obtained by extracting the yak bean fermented with the strain with water, C1 to C4 lower alcohol, or a mixture thereof. For example, the lower alcohol used in the extraction of the yak bean may be ethanol or methanol. During extraction, after slicing the yak bean, water, alcohol, or a mixture thereof may be extracted by adding 2 to 20 times the weight of the yak bean. For example, it may be extracted by adding 3 to 10 times. Extraction temperature is 30 ℃ to 100 ℃, 60 ℃ to 100 ℃. It may be 30 ℃ to 90 ℃, 30 ℃ to 80 ℃, 60 ℃ to 90 ℃, or 60 ℃ to 80 ℃. The extraction time may be 1 hour to 10 hours, 2 hours to 8 hours, 2 to 5 hours, 3 to 7 hours, or 4 to 6 hours. As for the extraction method, cold sedimentation, ultrasonic extraction, or reflux cooling extraction method can all be used. The number of extractions may be 1 to 5 times, 2 to 4 times, and 3 to 5 times of repeated extraction.

The fermented soybean extract may be extracted with ethanol. For example, the fermented soybean extract may be extracted with 60 to 80% ethanol. In one embodiment, as a result of comparing the hair loss improvement and hair growth promotion effect according to the extraction conditions of the fermented soybean fermented product, both the hot water fermentation extract and the fermentation extract extracted with 5% to 70% ethanol exhibited excellent effects, and in particular, It was confirmed that the fermented extract extracted with 70% ethanol showed the highest efficacy.

As used herein, the term "improvement" may mean all actions of at least reducing the severity of a parameter related to remission or treatment of a condition, for example, symptoms.

The term "improving hair loss" as used herein may comprehensively mean a process for treating, alleviating, or alleviating a hair loss condition, or its effect, for example, regeneration of hair follicle cells, restoration of activity of hair follicle cells, hair follicle cells It may mean any action that inhibits the progression of hair loss, such as inhibition of apoptosis and inhibition of expression of hair loss inducing factors such as DKK-1.

The term "promoting hair growth" as used herein may refer to any action that increases the growth of hair in a hair follicle, for example, an effect of promoting the proliferation of hair follicle cells, activity of hair follicle cells, or growth of hair follicle cells It can mean any action that increases the total amount of hair, such as.

In one embodiment, it was confirmed that the fermented soybean product exhibited significantly improved hair loss improvement and hair growth promoting effect compared to non-fermented Yak bean extract, Yak bean fermented extract fermented with other types of lactic acid bacteria, or minoxidil, an existing hair loss treatment agent. .

The cosmetic composition may include fermented soybeans at a concentration of 1 to 100 μg/mL based on the total weight of the composition. For example, the fermented soybean product is 10 to 90 μg/mL, 15 to 85 μg/mL, 20 to 85 μg/mL, 20 to 80 μg/mL, 25 to 80 μg/mL, 30 to 80 μg/mL Or, it may be included in a concentration of 35 to 80 ㎍ / mL. At this time, when the fermented soybean product is less than the weight part, it is difficult to expect a distinct hair loss improvement and hair growth promoting effect, and when the fermented soybean product exceeds the weight part, it is difficult to show an increase in the apparent effect due to the increase in content, and the formulation and It may cause some problems in the stability of the product, and there may be problems such as toxicity in the body.

The cosmetic composition may include, without limitation, ingredients that are generally allowed in addition to the active ingredient, and may include conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and a carrier. If necessary, ingredients such as surfactants, antioxidants, preservatives, pH adjusters, moisturizers, and lubricants may be further added. In addition, the cosmetic 　 composition additionally includes a substance that can supplementaryly provide essential nutrients to the skin, and preferably includes a natural scent, 　 cosmetics scent, or plant extracts, but may contain adjuvants that are not limited thereto. .

The cosmetic composition can be used as a cosmetic or quasi-drug formulation. The formulation of the composition may be any form of formulation in which fermented soybeans or a pharmaceutically acceptable salt thereof can be blended as an active ingredient. Preferably, hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment as a formulation of cosmetics or quasi-drug products for improving hair loss and promoting hair growth. It can be manufactured in various forms such as solutions, sol gels, emulsions, oils, waxes, aerosols, etc., such as ment, eyebrow hair growth agent, eyelash 　 hair growth agent, eyelash nutritional supplement, or pet 　 shampoo 　 and pet rinse, etc. It may be used in the manufacture or processing of hair devices, but is not limited thereto.

The 　 hair loss improvement or hair growth promotion composition may contain various components formulated in a conventional 　 composition for preventing hair loss according to various formulations such as cosmetic compositions, shampoo compositions, quasi-drug compositions, etc. or suitable for the final purpose. For example, in the case of a shampoo formulation, it may contain any one or more of a synthetic surfactant as a cleaning component, a preservative, a thickener, a viscosity modifier, a pH modifier, a fragrance, a dye, a hair conditioning agent, and water. Among the synthetic surfactants, synthetic anionic surfactants are alkyl and alkyl ether sulfates, representative examples of which are sodium lauryl sulfate, ammonium lauryl sulfate, triethanolamine lauryl sulfate, sodium polyoxyethylene lauryl sulfate, ammonium polyoxyethylene lauryl sulfate, etc. There is this. In addition, among the synthetic surfactants, synthetic amphoteric surfactants are alkyl betamine and alkyl amidopropyl betaine, and representative examples are cocodimethyl carboxymethyl betaine, lauryl dimethyl carboxymethyl betaine, lauryl dimethyl alpha-carboxyethyl betaine. , Cetyl dimethyl carboxymethyl betaine, and cocamido propyl betaine. The nonionic surfactants are alkanol amide and amine oxide, and include lauryl diethyl amine oxide, palm oil alkyldimethyl amine oxide, lauric acid diethanolamide, palm oil fatty acid diethanolamide, palm oil fatty acid monoethanolamide, and the like.

In addition, the composition for improving hair loss or promoting hair growth may be used for pets in different formulations. It can be prepared in the form of a 　 shampoo containing the above composition, and it can be prepared by adding a neutral detergent having less irritation to the skin of a pet and excellent in moisturizing power. In this case, a strong hair loss prevention effect can be obtained by the composition.

Another aspect is a composition for improving hair loss or promoting hair growth comprising fermented soybean fermented product by inoculating Bifidobacterium animalis subsp. Lactis. LDTM 8102 (KCTC13392BP) strain as an active ingredient It provides a method for improving hair loss or promoting hair growth, comprising the step of applying to the skin of an individual. Among the terms or elements mentioned in the description of the composition, the same as those already mentioned are as described above.

As used herein, the terms “applying”, “administering”, and “applying” are used interchangeably and result in at least partial localization of a composition according to an embodiment to a desired site, or route of administration It may mean disposition of the composition according to an embodiment into the subject by.

Another aspect is to improve hair loss or promote hair growth, containing fermented soybeans as an active ingredient, inoculated and fermented with Bifidobacterium animalis subsp . Lactis LDTM 8102 (KCTC13392BP) strain in Yak bean. Provide a food composition. Details of the composition are as described above.

In the food composition for improving hair loss or promoting hair growth according to an embodiment, when the fermented soybean is used as an additive to the food composition, it can be added as it is or used with other foods or food ingredients, and is appropriate according to a conventional method. Can be used. The mixing amount of the active ingredient can be appropriately determined according to each purpose of use, such as prevention, health or treatment.

The formulation of the food composition may be in the form of powders, granules, pills, tablets, capsules, as well as general foods or beverages.

The type of food is not particularly limited, and examples of foods to which the above substances can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and dairy products including ice cream. , Various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, and may include all foods in the usual sense.

In general, when preparing food or beverage, the fermented soybean may be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on 100 parts by weight of raw materials. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, the amount may be less than the above range, and the present invention has no problem in terms of safety in terms of using fractions from natural products. It can also be used in the above amount.

In the food composition according to an embodiment, the beverage may contain various flavoring agents or natural carbohydrates as an additional component, like a conventional beverage. The natural carbohydrates described above may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumatin and stevia extract, and synthetic sweeteners such as saccharin and aspartame can be used. The ratio of the natural carbohydrate may be about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the beverage according to the present invention.

In addition to the above, the food composition for improving hair loss or promoting hair growth according to an embodiment includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, It may contain stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages. In addition, the composition for improving hair loss or promoting hair growth of the present invention may contain pulp for the production of natural fruit juice, fruit juice beverage and vegetable beverage. These ingredients may be used independently or in combination. The ratio of these additives is not limited, but it is generally selected from 0.01 to 0.1 parts by weight based on 100 parts by weight of the food composition.

Another aspect is to improve hair loss or promote hair growth, which contains fermented soybean fermented product, which was fermented by inoculating Yak bean with Bifidobacterium animalis subsp . Lactis LDTM 8102 (KCTC13392BP) strain as an active ingredient. It provides a feed composition for use. Details of the composition are as described above.

The feed 　 composition is an organic acid such as citric acid, fumaric acid, adipic acid, lactic acid in addition to the fermented soybean for administration; Phosphates such as potassium phosphate, sodium phosphate, and polymerized phosphate; One or more of natural antioxidants such as polyphenol, catechin, tocopherol, vitamin C, green tea extract, chitosan, and tannic acid may be mixed and used. In addition, a diluent, a dispersant, a surfactant, a binder, or a lubricant may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., capsules, granules or tablets.

In addition, the 　 feed 　 composition is an auxiliary ingredient, various auxiliary agents such as amino acids, inorganic salts, vitamins, antioxidants, antifungal agents, antibacterial agents, and vegetable proteins such as crushed or crushed wheat, barley, corn 　 feed, blood meal, meat meal, fish meal, etc. In addition to the main ingredients such as animal protein and feed, animal fat and vegetable fat, it can be used with nutritional supplements, growth promoters, digestive and absorption promoters, and disease prevention agents.

When the 　 feed 　 composition is used as a 　 feed 　 additive, the 　 feed 　 composition may be added as it is or used with other ingredients, and may be appropriately used according to a conventional method. The dosage form of the feed composition can be prepared as an immediate release or sustained release formulation in combination with a non-toxic pharmaceutically acceptable carrier. Such edible carriers may be corn starch, lactose, sucrose, propylene glycol. In the case of a solid carrier, it may be a dosage form such as a tablet, a powder, or a toroki agent, and in the case of a liquid carrier, a dosage form such as a syrup, a liquid suspension, an emulsion, or a solution may be used. In addition, the administration agent may contain a preservative, a lubricant, a solution accelerator, a stabilizer, and other inflammatory disease ameliorating agents and substances useful for virus prevention.

The 　 feed 　 composition can be applied to a number of animal diets, that is, 　 feed, including mammals, poultry, fish and crustaceans. It can be used for commercially important mammals such as pigs, cows, goats, zoo animals such as elephants and camels, and livestock such as dogs and cats. Commercially important poultry includes chickens, ducks and geese, and may include commercially raised fish and crustaceans such as trout and shrimp.

Another aspect is for the prevention or treatment of hair loss containing fermented soybeans as an active ingredient, which was fermented by inoculating Yak bean with Bifidobacterium animalis subsp . Lactis LDTM 8102 (KCTC13392BP) strain. Provides a pharmaceutical composition. Details of the composition are as described above.

"Hair loss", which is a disease to be prevented or treated by the pharmaceutical composition, refers to a state in which there is no hair in the area where the hair should normally exist, and specifically, it may mean that the hair on the scalp (coarse black hair) falls out. have. The cause of hair loss is not limited, but may be caused by a variety of dietary and environmental influences such as genetic causes, hormonal imbalance, mental stress, exposure to air pollution, and consumption of processed foods. For example, hair loss includes hereditary androgenic 　 alopecia (baldness), circular 　 hair loss, tinea head due to fungal infection, telogen 　 hair loss, hair growth wall, hair growth disorders, etc., and scar formation 　 hair loss is caused by lupus. Hair loss, spontaneous folliculitis, lichen planus, burns and trauma can mean hair loss.

According to an embodiment, the fermented soybean product showed an effect of promoting proliferation, activity, or growth of hair follicle cells, an effect of inhibiting hair follicle cell death, and an effect of inhibiting expression of hair loss-related factors. Therefore, the fermented soybean can be used as an active ingredient in a pharmaceutical composition for preventing or treating hair loss.

In the present specification, the term "pharmaceutical composition" refers to a product prepared for the purpose of preventing or treating a disease, and may be formulated and used in various forms according to each conventional method. For example, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, and the like, and can be formulated in the form of external preparations, suppositories, and sterile injectable solutions. Specifically, it may be formulated and used in a form suitable for eye drop administration, for example, eye drops, creams, ointments, gels or lotions.

The pharmaceutical composition may be prepared in the form of a pharmaceutical composition for preventing or treating wounds, further comprising an appropriate carrier, excipient, or diluent commonly used in the preparation of pharmaceutical compositions, wherein the carrier is a non-natural carrier. naturally occuring carrier).

Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils.

In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient, such as starch, calcium carbonate, in the upper leaf extract and its fractions. , Sucrose (sucrose) or lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include water and liquid paraffin, which are simple diluents commonly used for suspensions, liquid solutions, emulsions, and syrups, and various excipients such as wetting agents, sweetening agents, fragrances, and preservatives. have. Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and suspension, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like may be used.

The composition according to an aspect may be used to improve hair loss or promote hair growth. Specifically, by promoting the proliferation or activity of dermal papilla cells (or hair follicle cells), inhibiting the death of dermal papilla cells, or inhibiting the expression of DKK-1 (Dickkopf-related protein 1), a hair loss inducing factor, it is remarkable in improving hair loss or promoting hair growth. It can have an effect.

1 is a view schematically showing a process for preparing a fermented soybean product using Bifidobacterium animalis subsp. Lactis. LDTM 8102 (Accession No. KCTC13392BP).
Figure 2 is a view showing the results of comparing the proliferation ability of dermal papilla cells when treated with an alcohol extract (EFY20, EFY40, or EFY80) of fermented soybeans of Example 1, or minoxidil (MX) as a positive control.
3 is a fermented soybean fermented alcoholic extract of Example 1 (EFY80), or a fermented soybean extract prepared using different lactic acid bacteria from Example 1, Bifidobacterium animalless subspecies. It is a view showing the result of comparing the proliferation ability of dermal papilla cells when treated with Yak Soybean fermented extract (Bif80) prepared using Lactis KCTC 5854 and Yak Soybean fermented extract (LGG) prepared using Lactobacillus rhamnosus GG.
4 is a non-fermented yak bean extract (UFY80), the hot water extract of the yak bean fermented product of Example 1 (WFY80), the alcohol extract of the yak bean fermentation of Example 1 (EFY), or minoxidil (MX) proliferation ability of dermal papilla cells when treated It is a diagram showing the result of comparison.

Hereinafter, a preferred embodiment is presented to aid the understanding of the present invention. However, the following examples are provided for easier understanding of the present invention, and the contents of the present invention are not limited by the following examples.

[Example]

Example 1. Preparation of fermented soybeans

A fermented soybean product was prepared using Bifidobacterium animalis subsp. Lactis. LDTM 8102 (accession number: KCTC13392BP) isolated from the feces of Korean infants. Specifically, mixed with glycerol and stored frozen at -80 ℃ Vitidobacterium animalis subsposis lactis LDTM 8102 (accession number: KCTC13392BP) preservation solution was inoculated in MRSC medium, 37 ℃, 20 to 24 hours in anaerobic conditions The entire culture process was performed while standing. Thereafter, 50 g/L of yak bean powder was inoculated with the strain culture solution having increased activity by performing the entire cultivation process, followed by shaking culture under anaerobic conditions maintained at 37°C. Samples collected in the fermentation process were lyophilized through a heat treatment process, and 0, 5, 10, 30, 50, and 70% fermented alcohol (vegetable alcohol) were added to the sample at a rate of 10 ml per 0.5 g. After bathing at °C for 2 hours, each was extracted. Thereafter, it was allowed to stand at room temperature for about 1 hour to cool, and then centrifuged at 5000 rpm for 10 minutes to separate the supernatant. The separated supernatant was filtered using a 0.2 µm syrine filter, and all alcohol was removed in a vacuum concentrator at 40°C, and then lyophilized to powder. Thereafter, the lyophilized powder was dissolved in 50% DMSO and used as fermented soybeans. Fig. 1 shows an overall process for preparing a fermented soybean product using Bifidobacterium animalis subspesis lactis LDTM 8102 (accession number: KCTC13392BP).

Comparative Examples 1 and 2. Preparation of fermented soybeans

With the exception of Bifidobacterium animal-less sub-Made System (Bifidobacterium animalis subsp. Lactis) was using a KCTC5854 lactic acid (Bif) or Lactobacillus ramno suspension GG (Lactobacillus rhamnosus GG) lactic acid (LGG) fermenting yakkong points, The fermented soybean products of Comparative Examples 1 and 2 were prepared in the same manner as in Example 1.

[Experimental Example]

Experimental Example 1. Comparison of hair loss improvement and hair growth promotion effect of fermented soybeans with existing hair loss treatment agents

In order to confirm the hair loss improvement and hair growth promoting effect of the fermented soybean fermented product of Example 1, the alcohol extract of the fermented soybean fermented product of Example 1 was treated with human dermal papilla cells (HDPC) to measure cell proliferation ability, The efficacy of this was compared with the existing hair loss treatment, Minoxidil (MX). Human dermal papilla cells were treated with 70% alcohol extract (EFY20, EFY40, EFY80) or minoxidil (MX) of the fermented soybean of Example 1, respectively, and then the cell proliferation ability was confirmed through MTT Assay.

Specifically, human dermal papilla cells were seeded in a 96-well plate using DMEM medium containing 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin at 37°C and 5% CO² ( Seeding). After incubation for 24 hours in a 37°C, 5% CO ₂ incubator, it was changed to a serum-free medium and further cultured for 18 hours. Then, the alcohol extract of the fermented soybean fermented product of Example 1 was treated in a serum-free medium at a concentration of 20, 40, or 80 μg/mL, and minoxidil was treated with 10 μM. Thereafter, after incubation for 48 hours, treatment with 110 μl of 5 mg/mL of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent, 37° C., 5% Incubated for 1 hour under CO2 humid conditions. During culture, purple crystals are formed in living cells. After incubation for 1 hour, the medium was removed, and 100 μl of DMSO was added to dissolve the crystal in a shaking incubator for 10 minutes, and the absorbance was measured at 570 nm. The test was repeated 3 times.

Figure 2 is a result of comparing the effect of increasing the proliferation ability of human dermal papilla cells by fermented Yak-kong of Example 1 (EFY: 70% EtOH extract of fermented Yak-kong with Bifidobacterium animalis subsp. Lactis LDTM 8102 ), and minoxidil (MX) It is a figure shown.

As a result, as shown in Figure 2, even when treated with minoxidil, which is an existing hair loss treatment, the dermal papillary cell proliferation power slightly increased compared to the untreated control group (Cont), but compared to the minoxidil treatment group, the Bifidobacterium animalis of the present invention It was confirmed that the proliferation of dermal papilla cells was remarkably increased when the fermented Yak bean fermented product (Example 1) was fermented using the subspecies lactis LDTM 8102 strain. In addition, it was confirmed that the fermented soybean product of Example 1 increased dermal papilla cell proliferation in a concentration-dependent manner.

That is, from the above results, it can be seen that the fermented product of Yak-bean according to one aspect not only shows excellent hair loss improvement and hair growth promoting effect in a concentration-dependent manner, but also shows significantly increased efficacy compared to existing hair loss treatment agents.

Experimental Example 2. Comparison of the effect of improving hair loss and promoting hair growth of fermented soybean products according to types of lactic acid bacteria

In order to compare the effect of improving hair loss and promoting hair growth of the fermented Yak Soybean according to the type of lactic acid bacteria used in fermentation, the proliferation power of dermal papilla cells was measured in the same manner as described in Experimental Example 1. Specifically, the alcohol extract of the fermented soybean fermented product of Example 1 prepared using Bifidobacterium animalis subspasis lactis LDTM 8102, Bifidobacterium animalis subspesis KCTC5854 lactic acid bacteria (Bif) or Lactobacillus rhamnosus After treatment with the alcohol extracts of fermented soybeans of Comparative Examples 1 and 2 prepared using GG lactic acid bacteria (LGG), the proliferation ability of human dermal papilla cells was compared.

3 is a fermented soybean fermented alcoholic extract of Example 1 (EFY80), or a fermented soybean fermented extract prepared using different lactic acid bacteria from Example 1, Bifidobacterium animalless subspecies. It is a view showing the result of comparing the proliferation ability of dermal papilla cells when treated with Yak Soybean fermented extract (Bif80) prepared using Lactis KCTC 5854 and Yak Soybean fermented extract (LGG) prepared using Lactobacillus rhamnosus GG.

As a result, as shown in FIG. 3, when the fermented extracts of Comparative Examples 1 and 2 were treated, the dermal papilla cell proliferation capacity was slightly increased compared to the untreated control group, but the effect was insignificant, compared to the existing hair loss treatment, minoxidil. Nor did not show any significant effect. In contrast, when the fermented yak bean fermented product (Example 1) fermented using the Bifidobacterium animalis subspasis lactis LDTM 8102 strain of the present invention was treated, compared to the yak bean fermented product prepared using other lactic acid bacteria It was confirmed that it significantly increased the proliferation power of dermal papilla cells.

Experimental Example 3. Comparison of hair loss improvement and hair growth promotion effect of fermented soybeans according to extraction conditions

In order to compare the hair loss improvement and hair growth promotion effect of the fermented Yak Soybean according to the extraction conditions, dermal papilla cell proliferation was measured by the same method as described in Experimental Example 1. Specifically, dermal papilla cells after treatment with the hot water extract (WFY80) of the fermented yak bean of Example 1, the alcohol extract of the non-fermented yak bean (UFY80), and the alcohol extract (EFY) of the fermented yak bean of Example 1 at 80 μg/ml The proliferative capacity was compared.

4 is a non-fermented yak bean extract (UFY80), the hot water extract of the yak bean fermented product of Example 1 (WFY80), the alcohol extract of the yak bean fermentation of Example 1 (EFY), or the proliferation ability of dermal papilla cells when treated with minoxidil (MX) It is a diagram showing the result of comparison.

As a result, as shown in Figure 4, the hot water extract of the fermented yak bean exhibited a similar level of dermal papilla cell proliferation effect as the non-fermented yak bean extract, but the alcohol extract of the fermented yak bean was treated with the non-fermented yak bean extract It was confirmed that it showed a significantly higher level of dermal papilla cell proliferation effect. Therefore, it can be seen from the above results that the effect of improving hair loss and promoting hair growth is the most excellent when the 70% alcohol extract of the fermented soybean is treated.

Claims (9)

Yak bean fermented by inoculating Bifidobacterium animalis subsp. Lactis. LDTM 8102 (KCTC13392BP) strain as an active ingredient. The extracted cosmetic composition for improving hair loss or promoting hair growth. The cosmetic composition of claim 1, wherein the fermented product comprises a fermented filtrate or a fermented extract. The cosmetic composition of claim 2, wherein the fermentation extract is extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. delete The cosmetic composition of claim 1, wherein the hair loss improvement or hair growth promotion is achieved by promoting activity of dermal papilla cells, promoting proliferation of dermal papilla cells, or inhibiting death of dermal papilla cells. The method according to claim 1, wherein the composition is a skin external preparation formulation, hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, eyebrow hair growth agent , Eyelash 　 hair growth agent, a composition having a formulation of eyelash nutritional. Yak bean fermented by inoculating Bifidobacterium animalis subspecis lactis LDTM 8102 (KCTC13392BP) strain as an active ingredient, and the fermented yak bean was extracted with ethanol, improving hair loss and hair growth Food composition for promotion. Yak bean fermented by inoculating Bifidobacterium animalis subspecis lactis LDTM 8102 (KCTC13392BP) strain as an active ingredient, and the fermented yak bean was extracted with ethanol, improving hair loss and hair growth Feed composition for promotion. Yak bean fermented by inoculating Bifidobacterium animalis subspecis lactis LDTM 8102 (KCTC13392BP) strain as an active ingredient, and the fermented yak bean was extracted with ethanol, preventing or treating hair loss Pharmaceutical composition for use.

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