KR101133785B1 - Composition for Preventing Hair Loss and Promoting Hair Growth Containing Scultellaria baicallensis and Method of Preparing the Same - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and wool promotion and a method for producing the same, and more specifically, golden extract, sewage extract, barberry extract, machiko extract, ginkgo biloba extract, white leaf extract, red ginseng extract and licorice extract It relates to a composition for preventing hair loss and promoting wool containing.

Hair loss prevention and wool promotion composition according to the present invention is made of natural products have no side effects to the human body, as well as smooth blood circulation to the hair root cells, scalp protection and hair nourishment function has excellent effects in preventing hair loss and promoting wool have.

Description

Composition for Preventing Hair Loss and Promoting Wool Containing Gold and Manufacturing Method Thereof {Composition for Preventing Hair Loss and Promoting Hair Growth Containing Scultellaria baicallensis and Method of Preparing the Same}

The present invention relates to a composition for preventing hair loss and promoting wool containing gold and a method for producing the same.

Hair protects and protects the body from external stimuli such as buffering and external stimuli such as ultraviolet rays, temperature, and friction, and absorbs heavy metals such as arsenic, mercury, and zinc that are unnecessary to the body and releases them to the body. It is an important aesthetic factor in the expression of external images.

In modern society, physical rhythm is caused by various causes such as physical, mental, genetic, and environmental factors such as side effects from stress, environmental pollution, drug addiction and misuse, perm, dyeing, use of hair dressings and genetic factors. It is broken and unbalanced, not only the scalp but also hair follicles are greatly affected, resulting in various forms such as hair loss as well as dry or sensitive hair loss.

Currently, the cause of hair loss is caused by a number of factors. First, male hormone testosterone, DHT (5 alpha-dihydrotestosterone), is highly active in the hair follicle due to the overproduction of hormones by genetic factors. DHT binds to androgen receptors to slow down the hair follicle protein synthesis, shorten hair follicle growth and shrink hair follicles to cause hair loss, or to cover hair follicles and nipples as an abnormal blood circulation supplied to the nipples If the amount of peripheral capillaries in the blood flow decreases and the nutrients are insufficient in the area of the papilla and basal cells, metabolism is reduced, causing hair growth abnormalities, or if dandruff occurs due to a decrease in physiological function of the scalp, Hair loss due to itching and inflammation of the scalp due to clogging and bacterial propagation Or hair loss due to abnormal blood growth caused by decreased blood flow in the peripheral blood vessels of the subcutaneous tissue of the head due to tension of the scalp, or other malnutrition, adverse effects caused by drugs, heredity, stress, environmental factors, etc. Hair loss is known to be caused by various factors.

As much attention is currently focused on hair care, researches and inventions on cosmetic compositions prepared using natural extracts (medicinal herb extracts) with little irritation and fewer side effects to prevent the occurrence of hair loss are actively progressed. As a patent application for hair loss prevention, wool, hair growth promoting composition, hair loss prevention composition containing herbal extract containing thorny skin, pine needles, pine flower, gyoza, fresh sulfur and hair loss (Korea Patent Publication No. 10-2009-0051586), There are a number of patent applications such as hair loss prevention compositions (Korean Patent Application Publication No. 10-2005-0014473) using natural products, such as hair growth promoting compositions having a bactericidal function containing Baekduong, Daesan, and golden extracts. Thanks to such active research, various cosmetic compositions such as hair lotion, hair shampoo, hair treatment, hair rinse and the like for preventing hair loss are widely distributed and used in the market, but conventional cosmetic compositions commonly distributed are substantially There were no products with satisfactory effects on dandruff prevention and hair loss prevention, and there were problems that caused various side effects or failed to form healthy hair growth.

In addition, since the product of the composition used for the scalp or hair is frequently used for a long time, it should not show side effects such as irritation or damage, and existing commercially available products often cause side effects on the scalp because the main ingredient is a chemical. many. Recently, hair loss treatments such as minoxidil and propecia have been developed, and anti-hair loss effects are expected. However, the application of such hair loss treatments causes unexpected side effects such as decreased sexual function, allergies, depression, and local inflammation due to imbalances in improvement of the body. The risk of application is increasing.

The inventors of the present invention, while studying hair loss prevention and hair growth cosmetic composition containing herbal extracts, complex composition containing natural products such as gold, without side effects, as well as blood circulation to the hair root cells, scalp protection, hair nutrition The present invention has been completed by confirming that a systematic effect such as feeding is to prevent hair loss and promote wool.

Accordingly, it is an object of the present invention to provide a composition for preventing hair loss and promoting wool containing golden extract, sewage extract, barberry extract, chome extract, ginkgo biloba extract, cypress leaf extract, red ginseng extract and licorice extract.

Still another object of the present invention is to provide a method for preparing hair loss prevention and wool promotion composition containing golden extract, sewage extract, bastard extract, guchi extract, ginkgo biloba extract, cedar leaf extract, red ginseng extract and licorice extract.

In order to achieve the above object, the present invention provides a composition for preventing hair loss and promoting wool containing golden extract, sewage extract, barberry extract, guchi extract, ginkgo biloba extract, cypress leaf extract, red ginseng extract and licorice extract.

The present invention also comprises the steps of: (a) hot water extraction by mixing golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng and licorice; And (b) cooling the steam generated by heating the hot water extract to obtain a distillate to prepare a composition for preventing hair loss and promoting wool, and preparing a composition for preventing hair loss and promoting wool.

The present invention also comprises the steps of: (a) hydrothermal extraction of each of the golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng and licorice; And (b) cooling the steam generated by heating the respective hydrothermal extracts to obtain respective distillates, and then mixing them to prepare a composition for preventing hair loss and promoting wool, including preparing a composition for preventing hair loss and promoting wool. Provide a method.

Hair loss prevention and wool promotion composition according to the present invention is made of natural products have no side effects to the human body, as well as smooth blood circulation to the hair root cells, scalp protection and hair nourishment function has excellent effect in preventing hair loss and promoting wool have.

The present invention relates to a composition for preventing hair loss and promoting wool containing golden and a method for producing the same, including sewage extract, barberry extract, machiko extract, ginkgo biloba extract, cypress leaf extract, red ginseng extract, licorice extract and the like as an active ingredient. It relates to a composition for preventing hair loss and promoting wool containing natural ingredients.

The present invention relates to a composition for preventing hair loss and promoting wool containing golden extract, sewage extract, bastard extract, guchi extract, ginkgo biloba extract, cypress leaf extract, red ginseng extract and licorice extract.

Specifically, the present invention, the composition is 10-30 parts by weight of golden extract, 1-25 parts by weight of sewage extract, 1-20 parts by weight of barberry extract, 1-20 parts by weight of Machiko extract, 0.01-20 parts by weight of ginkgo leaf extract, It may be characterized by containing 1 to 25 parts by weight of the baekbaek leaf extract, 1 to 25 parts by weight of ginseng extract and 1 to 20 parts by weight of licorice extract.

Preferably, the content of the golden extract is 18 to 22 parts by weight, the content of the sewage extract is 13 to 17 parts by weight, the content of the individual extract is 8 to 12 parts by weight, the content of the extract of Machi Prefecture is 8 ～ 12 parts by weight, the content of the ginkgo biloba extract is 3-7 parts by weight, the content of the cypress leaf extract is 13-17 parts by weight, the content of the red ginseng extract is 13-17 parts by weight, the content of the licorice extract It is 8-12 weight part.

If the content of each extract is less than the content range, the effect of preventing hair loss and promoting wool is insignificant, and if the content exceeds the weight range, the formulation and stability may be adversely affected.

Here, it is preferable that a golden extract contains 10-30 weight part with respect to 100 weight part of compositions. If the content of the golden extract is less than 10 parts by weight, hair loss prevention and wool promoting effect is insignificant, and if it exceeds 30 parts by weight, the production cost of the composition is not preferable to increase.

The content of the sewage, individual fruit, machi, ginkgo biloba, ginseng leaf, red ginseng and licorice extract is 1 to 30 parts by weight, respectively, if less than 1 part by weight, the hair loss improvement effect is insignificant, and if the content exceeds 30 parts by weight It is not preferable.

In the present invention, the golden extract, sewage extract, bastard extract, guchi extract, ginkgo biloba extract, cypress leaf extract, red ginseng extract and licorice extract can be obtained by hot water extraction or ethanol extract, for example, water, 1-4 Anhydrous or hydrous lower alcohols, mixed solvents of the lower alcohols with water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol and the like, or by other extraction methods known in the art. .

Hereinafter, each natural product component contained in the composition of the present invention will be described in detail.

Golden ( Scultellaria baicallensis ) is dried from the root of golden, a perennial herb belonging to the Lamiaceae family, and has the effect of dehumidification fever , hemostasis, and anemia. This medicine is used for the treatment of hypertension and epidemic meningitis (Kim Dong-chan, Roh Seung-hyun, Lee Sang-in, Lee Young-jong, Ju Young-seung). : Yeonglin Co., Seoul, 1990, pp.111-113, pp263-264, p338). It contains several types of flavonoids, such as wogonin, baicalin, and baicalein.The pharmacological effect is to reduce the heat of the heart and lungs or to stop coughing and blood. It has an effect. The skin has anti-allergic, antioxidant, and anti-inflammatory effects, and is excellent in removing harmful oxygen.

Synao ( Cynanchum silfordii Hemsley ) is a perennial vine herb that belongs to Yeowi-gu and is recognized for its weakness compared to wild ginseng. Sewage looks like sweet potato, but slightly smaller, and when ginseng is precious, instead of ginseng, it is used as a substitute for sukji, or because there is less contraindication and better digestion than sukji. Sewage acts as a function of donkey and succinct amber to produce blood and removes inflammation, boils and swelling. In addition to sputum and phlegm, it treats postpartum disease, cold and lobular treatment of women, and fills bone marrow. There is a function to strengthen the musculature. When used as a life to communicate the energy to facilitate the communication of faeces and detoxification action, and steaming properly or boiled and then dried to write the liver and gods to create blood courses. For the purpose of blackening hair, black beans and black sesame seeds are recommended for use. In particular, it effectively absorbs ultraviolet rays and is effective as an anti-aging agent for scalp and hair, and is effective in preventing skin and scalp aging through antioxidant and astringent effects. It contains anthraquinone derivatives, the main constituents of which are chrysophanic acid, chrysophanol, emodin, anthronene, rhein, starch, It is used in skin diseases such as eczema, suppresses skin pruritus caused by allergy, shows anti-aging effect through antioxidant and astringent effect, blackens gray hair and effectively absorbs UV-B area of scalp and prevents aging of scalp and hair.

Sinapis Semen is a dried seed of Brassica juncea, a herbaceous genus belonging to the Cruciferaceae. The major physiologically active compounds are sulfur compounds such as sinigrin, 8- (methylsulfonyl) octyl glucosinolate, and phytoalexin include brassinin and cyclobrassinin. It is used for increasing skin human action, topical stimulant and foaming medicine. It has appetite and antiseptic effect.

Portulaca oleracea consists of l-noradrenaline, dopamine, alkaloid, potassium salt, organic acid (apple acid, lemon acid, ivory acid), amino acids (glutamic acid, aspartic acid, alanine). The dry matter is crude protein 4.5-6.6%, crude fat 3.7-5.8%, crude ash 25-27%, monosaccharides 2-3%, candy 4.4-6%, water-degradable polysaccharides 9-14%, starch 0.2 ~ 2.5%, vitamins B1 and C, carotene 9.4 ~ 17mg%, nicotinic acid 25 ~ 50mg%, tocopherol 2 ~ 6mg%, trace elements zinc, copper, manganese, nickel, iron, etc., soothing skin irritation and allergic reaction It has the effect of preventing aging, antibacterial and antifungal by action, prevention of skin dryness and anti-inflammatory action.

Ginkgo biloba extract is extracted from Ginkgo biloba L., a deciduous tree belonging to the Ginkgo family, and Ginkgo biloba leaves contain flavonoids with free radicals, antioxidants, cell membrane protection, vascular diseases, and blood pressure lowering. In addition, it contains various substances that improve blood circulation, remove blood clots, prevent blood aging, inhibit skin aging, protect dry skin and prevent fine wrinkles on the face. In addition, it has an antiallergic effect as well as antibacterial action. In general, ginkgo biloba extract is composed of flavonoids and aromatic oxycarboxylic acids of the leaves and contains active ingredients such as prabon glycosides, ginkonic acids, ginkgo gettins, ginkgoides A, B, C, M, and tefenlactones. It is included.

The main components exhibiting biological activity contained in the ginkgo biloba extract are flavonoid compounds, terpenes, and tannins, proanthocyanidins, and alkylphenols. Flavonoids of ginkgo biloba can be chemically classified into flavonol glycosides and biflavones. Biflavones include sciadopitysin, ginkgetin, and isotizing (isoginkgetin), bilobetin, and amentoflavone have been reported, and flavonol glycosides include kaempferol, quercetin and isoram, depending on the type of aglycone. It can be roughly classified as a glycoside of isorhamnetin. Terpene compounds are known as ginkgolides A, B, C and J and bilobalides. Among these components, especially isorhamnetin and quercetin dilate coronary vessels to promote blood circulation in the scalp, and ginkgetin lowers blood cholesterol and significantly lowers blood pressure. . They also have antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and skin fungi, so that the extract of ginkgo biloba can be added to the hair composition to prevent dandruff and to remove the itching of the scalp.

Cacumen biotae consists of quercetin, myricetin, fenchone, alpha-pinene, alpha-caryophyllene, and tujen It contains thujone and hinokiflavone, and is effective for antibacterial, bactericidal, hemostatic, hair growth, follicular regeneration, and hair whitening and hair loss.

Ginseng ( Sophorae Radix ) is a perennial herb and refers to its roots and is harvested in spring and autumn. Roots include alkaloids d-matrine, d-oxymatrine, d-sophoranol, l-methylcytisine, l-baptifoline and l -Sopocarpine is contained, and flavonoids such as xanthohumol, isoxanthohumol and camphorol are contained. Main effects include diuretic and antibacterial effects.

Licorice ( Glycyrrhiza glabra ) is a soybean and perennial herbaceous plant that has excellent anti-inflammatory, anti-allergic and antibacterial effects, and has an excellent effect on collagen synthesis and hyaluronic acid synthesis, which are closely related to skin elasticity, wrinkle improvement and skin moisturizing effect. The main components of the physiological activity of licorice are glycyrrhizin, flavonoids and mannitol.

The composition for preventing hair loss and promoting hair of the present invention may add a base component that can be blended into general scalp and hair external preparations as needed. Specific examples include dissolution aids, surfactants, moisturizers, thickeners, pH adjusters, preservatives, antioxidants, metal ion sequestrants, fungicides, anti-inflammatory agents, antimicrobial agents, solvents, colorants, or flavoring agents.

Here, the dissolution aid may be specifically isopropyl myristate, polyethylene glycol, medium chain fatty acid triglycerides, hydrocarbons, glycols and the like.

Among the surfactants, anionic surfactants are specifically ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium Lauryl ether sulfate (1 to 3 ethylene oxide) and the like. Among the surfactants, nonionic surfactants may specifically be polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene, hardened castor oil derivatives, fatty acid diethanolamides, glyceryl stearate, and the like. The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like. Among the surfactants, the amphoteric surfactant may be specifically betaine, amidebetaine type, sulfobetaine type, steartrimonium chloride, or the like.

Moisturizing agent may be specifically glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol and the like.

The thickener may specifically be a water-soluble high molecular compound such as methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxymethyl cellulose, hydroxymethyl cellulose and the like.

The pH adjusting agent may specifically be citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid and the like.

Preservatives may specifically be benzoic acid, paraoxybenzoic acid esters, mixtures of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin and the like.

Antioxidants may specifically be dibutylhydroxytoluene, ascorbic acid and the like.

Specifically, the metal ion blocking agent may be ethylenediaminetetraacetate acetate, ethylenediaminetetraacetate tetrasodium, or the like.

Fungicides may specifically be chlorhexidine gluconate, quaternary ammonium salts, pyroxtonolamines, zinc pyrithione suspensions, iodopropynyl butylcarbamate, salicylic acid and the like.

The anti-inflammatory component may specifically be monoammonium glycyrrhinate, dipotassium glycyrizine, stearyl glycyrizinate, chamomile, alpha bisabolol, allantoin and mixtures thereof.

The antimicrobial component is specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, pyroxtonolamine, ketoconazole, arnica extract, iodopropynylbutylcarbamate, benzalkonium chloride, benzenetonium chloride, benzoic acid and salts thereof, benzyl alcohol, lavender, Rosemary, salicylic acid, triclocarban, zinc pyrithione suspensions and mixtures thereof and the like.

The solvent may be specifically ethanol, purified water, Tween 20, cyclomethicone, mineral oil, dimethicone, and the like.

Colorants and flavoring agents can use the bases used in general scalp and hair preparations.

Hair loss prevention and promotion of the composition of the present invention can be prepared in any formulation that can be applied to the scalp and hair in a liquid, cream, paste or solid form, etc., and to promote hair growth by adding a conventional additives Shampoos, hair conditioners, hair lotions, hair tonics, hair packs, blends of shampoos and rinses, liquid wool types, and the like, and also include aerosol types of these formulations. The composition for preventing hair loss and promoting wool is preferably used in a method of transdermal administration such as direct application or spraying to the scalp or hair.

In another aspect, the present invention comprises the steps of: (a) hot water extraction by mixing golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng and licorice; And (b) cooling the steam produced by heating the hot water extract to obtain a distillate to prepare a composition for preventing hair loss and promoting hair growth, including preparing a composition for promoting hair loss.

In another aspect, the present invention, (a) hydrothermal extraction of each of the golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng and licorice; And (b) cooling the steam generated by heating the respective hydrothermal extracts to obtain respective distillates, and then mixing them to prepare a composition for preventing hair loss and promoting wool, comprising the steps of: It relates to a manufacturing method.

In the manufacturing method, hot water extraction by mixing golden, sewage, barberry, machi, ginkgo biloba, cypress leaves, red ginseng and licorice, or hot water extraction of each of the golden, sessau, barberry, machi, ginkgo biloba, cypress leaves, ginseng and licorice can do.

The weight ratio of each natural product is 10-30 parts by weight of golden extract, 1-25 parts by weight of sewage extract, 1-20 parts by weight of barberry extract, 1-20 parts by weight of Machi Prefecture extract, 0.01-20 parts by weight of ginkgo biloba extract, white leaf extract 1 ˜25 parts by weight, 1 to 25 parts by weight of red ginseng extract and 1 to 20 parts by weight of licorice extract, preferably 18 to 22 parts by weight of golden extract, 13 to 17 parts by weight of sewage extract, 8 to 12 parts by weight of barley extract, Machi 8 to 12 parts by weight of extract, 3 to 7 parts by weight of ginkgo leaf extract, 13 to 17 parts by weight of white leaf extract, 13 to 17 parts by weight of red ginseng extract, 8 to 12 parts by weight of licorice extract.

In addition, after the hot water extraction, the materials used are removed, the hot water extract is heated to 80 ~ 100 ℃, the steam is obtained by using a cold water cooling tube of 4 ~ 10 ℃. Here, the distillate may be further centrifuged to remove solids, and then the composition may be obtained. In addition, the distillate may be centrifuged at 12000 rpm to remove solids, and then the composition may be obtained. The hot water extraction method, centrifugation of the distillate, solids removal process will be applicable to a variety of methods widely used in the art.

The composition for preventing hair loss and promoting wool according to the present invention may be prepared in the form of a cosmetic composition that can be commonly applied to the scalp and hair. For example, it may be prepared by shampoo, hair treatment, hair tonic, hair pack, mixed type of shampoo and rinse.

In addition, the composition for preventing hair loss and promoting wool of the present invention may be in the form of a pharmaceutical composition. In the case of the pharmaceutical composition, it may be prepared by further containing a pharmaceutically suitable and physiologically acceptable adjuvant, wherein the adjuvant includes a solvent, a disintegrant, a sweetener, a binder, a coating agent, an expanding agent, a lubricant, a lubricant Solubilizers, such as an agent or a flavoring agent, can be used. In addition, in addition to the active ingredient described above for proper administration, it may be formulated to include one or more additional pharmaceutically acceptable carriers. Here, the pharmaceutically acceptable carrier may be used in combination with saline solution, sterile water, Ringer's solution, buffered saline solution, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, antioxidants, Other conventional additives such as buffers and bacteriostatic agents can be added.

In addition, the pharmaceutical composition for preventing hair loss and promoting wool may be formulated into injectable formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules or tablets by additionally adding diluents, dispersants, surfactants, binders and lubricants. Can be. In the usual manner via the intravenous, intraarterial, intraperitoneal, intramuscular, intraarterial, intraperitoneal, sternum, transdermal, nasal, inhalation, topical, rectal, oral, intraocular or intradermal routes May be administered.

The pharmaceutical composition or the cosmetic composition is a form in which the distillate mixture of golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng, and licorice hot water extract of the present invention is effective in a suitable solvent. May be provided.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences, dosage forms, and forms such as age, degree of lesion, and the composition of the present invention is useful as a cosmetic, medicine, or quasi-drug for improving the scalp and hair condition. Can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Preparation of a composition for preventing hair loss and promoting wool according to the present invention

(1) In order to obtain a composition for preventing hair loss and promoting wool according to the present invention, 20 g of gold, 15 g of sewage, 10 g of barberry, 10 g of ginseng, 5 g of ginkgo biloba, 15 g of ginseng, 15 g of ginseng and 10 g of licorice After washing sufficiently with purified water, it was mixed. In hot water distillation, using 20L purified water, heated to 80 ~ 100 ℃ for 8 ~ 48 hours to obtain hot water extract, then remove the used materials and heat hot water extract to 90 ~ 100 ℃, 4 ~ 10 ℃. The distillate was obtained using the cold water cooling tube of the. The obtained mixed distillate was centrifuged at 4 ° C. for 30 minutes at 12000 rpm to remove solids, thereby obtaining a composition.

(2) In order to obtain a composition for preventing hair loss and promoting hair growth according to the present invention, gold, sewage, barberry, machi, ginkgo biloba, cypress leaves, red ginseng and licorice were separately extracted and distilled and mixed at the above weight ratio. .

Specifically, 1000 g of golden, sewage, individual, machi, ginkgo biloba, baekryeop, red ginseng and licorice were heated to 80 to 100 ° C. for 8 to 48 hours using 20 L of purified water to obtain a hydrothermal extract. After extraction, the used materials were removed and the hot water extract was heated to 90-100 ° C., each distillate was obtained using a 4-10 ° C. cold water cooling tube. Each of the obtained distillates was centrifuged at 4 DEG C for 30 minutes at 12000 rpm to remove solids. In order to obtain a composition 10L by mixing them, 0.1-3.0L of distillate of golden extract, 0.1-2.5L of distillate of sewage extract, 0.1-2.0L of distillate of bastard extract, 0.1-2.0L of distillate of March extract, A distillate of 0.001 ~ 2.0L of ginkgo biloba extract, 0.1-2.5L of distillate of cypress leaf extract, 0.1-2.5L of distillate of red ginseng extract and 0.1-2.0L of distillate of licorice extract were prepared.

Experimental Example 1 Test of Proliferation Activity of Skin Cells

In order to examine the proliferative activity of the skin cells for the composition according to the present invention, the human keratinocyte HaCaT cell line (Cell Lines Service, Germany) inoculated in a 24-well culture plate at a concentration of 1 × 10 ⁴ cells / ml It was. Medium was used DMEM (Dubelcco'S Modified Eagle Medium, BRL, USA) containing 10% bovine serum. After 48 hours of incubation in DMEM containing 10% FBS and incubated for 25-30% of the surface area of the culture vessel, the cells were further incubated for 24 hours by replacing with the FBS-free DMEM containing 0.5% to 10% of the composition prepared in Example 1. . After incubation, 50 μl of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazoleum bromide (MTT, Sigma M5655, USA) solution (2.5 mg / ml) was added, and 3 After further incubation for a period of time, the supernatant was removed, 200 μl of dimethyl sulfoxide (DMSO, Sigma D2650, USA) solution was added to each well, followed by stirring for 20 minutes to dissolve formazan crystals formed, followed by 100 μl. Taken into 96 wells, the absorbance was measured at 570 nm by Enzyme-Linked Immunosorbent Assay (ELISA). The degree of cytotoxicity was calculated according to the following formula based on the absorbance intensity of the control group using pure water and expressed as a percentage.

[Equation 1]

Cell proliferation effect (%) = (absorbance at the time of extract treatment / absorbance of the control group) × 100

[Table 1]

As a result, the composition of the present invention was found to be 1,78%, 3.28%, 15.69%, 32.06%, respectively, as compared to the control group, and showed much better cell proliferation and activity effects than the experimental group containing each extract.

Experimental Example 2: Antioxidant Test Using DPPH

In order to confirm the antioxidant effect of the composition according to the present invention prepared in Example 1, using the Blois method using DPPH (1,1-Diphenyl-2-picrylhydrazyl, Sigma) (Blois MS, Nature 181, pp1198-1200,1958), the radical scavenging effect of the composition was confirmed. DPPH is a relatively stable free radical, which is purple, exhibits maximum absorbance at 517 nm when it is in radical state, and loses absorbance when it is eliminated. Thus, antioxidant activity can be measured using this principle.

150 μl of a 0.2 mM DPPH solution (ethanol in the case of Blanc) was prepared to contain 0.5-10% of the composition prepared in Example 1, and 150 μl of these were added and mixed, and the mixture was left at room temperature for 30 minutes. The absorbance was measured at 517 nm, and the control group was set to the case using purified water. After absorbance measurement for the experimental group and the control group, the degree of antioxidant capacity was expressed as a percentage based on the absorbance intensity of the control group using pure water. Using the following formula 2 to obtain the free radical scavenging activity effect is shown in Table 2 below.

[Equation 2]

DPPH radical scavenging rate (%) = [(A-B) / A] × 100

(A: absorbance of the control group, B: absorbance of the reaction group when the experimental group was treated)

The results were as shown in Table 2 below.

TABLE 2

Rather than the antioxidant effect of each extract, the composition (mixture extract) according to Example 1 prepared by mixing eight extracts of golden, sewage, barberry, machi, ginkgo biloba, cypress leaf, red ginseng and licorice, the higher the content, the antioxidant effect Increased, especially when treated at 10%, with the best antioxidant effect.

Experimental Example 3: Animal Functional Evaluation of Wool Functional Evaluation of the Composition of the Present Invention

A hair score test using an animal test was conducted to verify the efficacy of the composition of the present invention obtained in Example 1.

As an animal test model for evaluating hair growth induction promoting effect, the C57BL / 6 mouse model was used. Is present only in hair follicles, and the melanin synthesis matches well with the hair growth cycle, which has the advantage of determining the hair growth cycle by the color of the skin. It is used.

42-56 days old mice (C57BL / 6, male) were used. First, the hair on the back of the mouse was removed using an electric razor, and each mouse was weighed and divided into 10 pieces so that the weight was evenly distributed. After the adaptation period for one day, the hair removal site was coated with a control group, 5% minoxidil, and hair loss prevention and wool growth promoting composition according to Example 1, wherein 35% ethanol was used as a control group. The concentrated solid of the hair loss prevention and wool growth promoting composition was used diluted with 35% ethanol. 100 µl each was applied to the back of the mouse per day for 15 days to observe the growth phase induction promoting action.

As shown in FIG. 1, after 3 days and 8 days, the composition treatment group according to the present invention was superior to the minoxidil treatment group and the control group, and after 15 days, the composition according to the present invention. In the case of the mice treated with, the hair loss site was recovered almost completely.

Experimental Example 4: Wool functional evaluation test of the composition of the present invention

Small scale clinical trials were conducted by selecting female and male alopecia patients through the basic formulations that can be commercialized with the present invention composite compositions that were primarily confirmed in animal experiments. Subjects were divided into two groups for 20 adult men and women with hair loss ranging from 30 to 60 years old (15 males, 5 females). 5% of minoxidil sold at the pharmacy 5 ml once a day Having a liquid tonic prepared from a product (prepared in modern medicine) and the composition of the present invention obtained according to Example 1 (concentrated solids of the hair loss prevention and wool growth promoting composition according to Example 1 diluted with 35% ethanol) 3 It was applied to the bald area for months and the result was observed.

The evaluation of the effect was performed by five stages of evaluation of daily hair loss, visual judgment, and improvement of subjective symptoms during trituration, and the overall improvement was taken as the average of the evaluation scales. According to one point unit, as shown in Table 3. The experimental group was divided into the minoxidil 5% group and the composition group of the present invention.

[Table 3] Score scale used in small clinical trials

[Table 4] Small scale clinical trial results

As can be seen from Table 3 and Table 4, as a result of small-scale clinical trials, the composition-treated group of the present invention showed 45 to 90% improvement in hair loss during visual judgment, subjective symptoms, and hair loss compared to the minoxidil-treated group. . Comprehensive improvement was found to have an average 70% improvement.

1 is a result of a hair score test using an animal experiment for the validation of the composition according to the present invention.

Claims (5)

Distillate of Golden Hot Water Extract, Distillate of Sewage Hot Water Extract, Distillate of Hot Water Extract of Algae, Distillate of Machi Prefectural Hot Water Extract, Distillate of Ginkgo Leaf Hot Water Extract, Distillate of Ginkgo Leaf Hot Water Extract, Distillate of Lime Ginseng Hot Water Extract, Licorice and Licorice Hair loss prevention and wool promotion composition containing the distillate of hot water extract. Hair loss prevention and wool promotion composition containing. According to claim 1, wherein the composition is 10 to 30 parts by weight of the distillate of golden hot water extract, 1 to 25 parts by weight of distillate of sewage hot water extract, 1 to 20 parts by weight of distillate of individual hot water extract, and distillation of hot water extract of Machi 1 to 20 parts by weight of liquid, 0.01 to 20 parts by weight of distillate of ginkgo biloba hot water extract, 1 to 25 parts by weight of distillate of cypress leaf hot water extract, 1 to 25 parts by weight of distillate of hot ginseng hot water extract and distillate of licorice hot water extract 1 A composition for preventing hair loss and promoting wool, comprising ~ 20 parts by weight. delete delete delete

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