KR101739229B1 - Composition for Preventing Hair Loss and Promoting Hair Growth and A method for manufacturing thereof - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth and, more specifically, to a composition for preventing hair loss and promoting hair growth, comprising Acorus calamus Linn., Mori cortex radicis, Glycyrrhiza uralensis, Pinus densiflora, Sophora angustifolia, Ligusticum chuanxiong Hort, Angelica gigas, Common purslane, guava, Asiatic pennywort, zedoaria, and Manchurian angelica, as active ingredients.

Description

TECHNICAL FIELD The present invention relates to a composition for hair loss prevention and hair growth and a method for manufacturing the same,

The present invention relates to a composition for hair loss prevention and hair growth, and more particularly, to a composition for prevention of hair loss and hair growth comprising iris, alfalfa, licorice, pine leaf, gosam, And compositions for hair growth.

Hair loss is a phenomenon that the hair grows easily because it does not grow normally, and the growing period is shortened and the resting period is lengthened. Hair normally comes out of the skin 50-100 times a day, but can be thought of as alopecia if it continues to fall off more than 100 a day.

Hair follicles are estimated to be over 100,000 hair follicles, and the density of hair gradually decreases with age. Hair loss is known to be an aging phenomenon, but the main factor is genetic and various factors such as social, psychological, mental stress and dietary habits act. The mechanism of hair loss caused by environmental stress such as stress is activated by immune cells and neutrophils, causing an inflammatory reaction and destroying hair follicle tissue, causing hair cell death, resulting in hair loss

Hair is a thread-like protein that grows in the hair follicles inside the dermis of the skin and grows. The hair growing from the hair follicle repeats growth and hair loss through four stages of hair cycle such as growth period, regressive period, rest period, and dropout period (Jain et al., 2012). The hair follicle grows in the dermis of the dermis of the growing period, and the dermis decreases in thickness of the dermis, so that the hair follicle becomes close to the epidermis and the dermal papilla cells in the hair follicle are eliminated and a new hair growth cycle is achieved.

Minoxidil-containing preparations are widely used as agents for the treatment and prevention of such alopecia. Minoxidil was originally developed as a hypertension treatment for blood pressure lowering, but it has become more famous as a hair growth promoter due to side effects caused by side effects. However, minoxidil was found to be unsuitable for long-term use due to side effects such as scalp irritation such as scalp drying, erythema, allergic contact dermatitis, facial hyperhidrosis and vasodilatation, hypotension caused by antihypertensive action, hypotension and tachycardia .

Conventional hair loss prevention and hair growth promoting agents that have been used so far do not show sufficient effects for prevention of hair loss and promotion of hair growth, and hair loss treatment agents such as minoxidil and propecia are not suitable for long-term use due to various side effects.

Therefore, there is a desire to develop a new scalp care composition for hair loss prevention and hair growth which is safe even for long-term use, has a simple manufacturing method, has an effect of preventing hair loss and promoting hair growth by improving the scalp condition, .

Accordingly, the present inventors have found that a composition for prevention of hair loss and hair growth can be obtained by using a composition comprising Mori cortex radicis, Acorus calamus Linn., Glycyrrhiza uralensis, Pinus densiflora, Sophora angustifolia, The present inventors confirmed that the mixed extract of Ligusticum chuanxiong Hort, Angelica gigas marjoram, Guava, Centella asiatica, Centella asiatica, and Bacteriophage has effects of preventing hair loss and baldness, treating inflammation, improving blood circulation, Respectively.

1. Korean Patent No. 10-1017709 2. Korean Patent No. 10-1017709 3. Korean Patent 10-1049776 4. Korean Patent No. 10-1451337

1. Aljuffali IA, Sung CT, Shen FM, Huang CT, Fang JY. 2014. Squarticles as a lipid nanocarrier for delivering diphencyprone and minoxidil to hair follicles and human dermal papilla cells. AAPS. J 16: 140-150. 2. B. Fu, H. Li, X. Wang, S. C. Frank and S. Cui, Isolation and identification of flavonoids in licorice and a study of their inhibitory effects on tyrosinase, J. Agric. Food Chem., 53: 7408-7414 (2005). 3. B. O. Botchkarev and J. Kishimoto, Molecular control of epithelial mesenchymal interactions during hair follicle cycling, J. Invest. Dermatol. Symp. Proc., 8, 46-53 (2003). 4. BS Park, SO Park, YS Ahn, GY Noh. Therapeutic effects of atobeauty cream in atopic dermatitis model mice induced with DNCB. Res J Med Sci 6: 71-76 (2012). 5. C. A. Jahoda, J. Whitehouse, A. J. Reynolds and N. Hole, Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages, Exp. Dermatol, 12: 849-859 (2003). 6. Cotsarelis G, Botchkarev V. Disorders of the hair and nails. In: Wolff K, Goldsmith LA, Katz SI, Gilchrest BA, Paller AS, Leffell DJ, editors. 2008. Fitzpatrick's dermatology in general medicine. 7th ed. New York: McGraw-Hill. 739-749. 7. C. Mou, B. Jackson, P. Schneider, P. A. Overbeek, D. J. Headon: Generation of the primary hair follicle pattern, Proc Natl Acad Sci. 103 (24): 9075-9080. (2006). 8. Dhurat R, Saraogi P. 2009. Hair evaluation methods: Merits and demerits. Int. J. Trichol. 1: 108-119. 9. D Jaybhaye, Varma S, Gagne N, Bonde V, Gite A, Bhosle D. Effect of Tectona grandis Linn. seeds on hair growth activity of albino mice. Int J Ayurveda Res 1: 211-215 (2010). 10. D. V. Mater, F. T. Kolligs A. A. Dlugosz and E. R. Fearon, Transient activation of β-catenin signaling in cutaneous keratinocytes is sufficient to trigger the active growth phase of the hair cycle in mice, Genes Dev, 10: 1219-1224 (2003). 11. E. M. Choi, Antinociceptive and antiinflammatory activities of pine (Pinus densiflora) pollen extrac, Phytother. Res., 21: 471-475 (2007). 12. G. Cotsarelis and S. E. Millar, Towards a molecular understanding of hair loss and its treatment, Trends Mol. Med., 7, 293-301 (2001). 13. H. S. Lee, S. J. Yoon, Y. K. Moon and J. Y. Moon, Hair growth effects of Mori Cortex radicis mixture on the hair of rat, Korean Society of Sericultural Science, 42: 83-85 (2000). 14. J. Y. Lee, K. R. Im T. K. Jung, M. H. Lee and K. S. Yoon, Medicinal herbal complex extracts for potential hair growth? promoting activity, J. Soc. Cosmet, 38: 277-287 (2012).

It is a principal object of the present invention to provide a composition for hair loss prevention and hair growth.

It is another object of the present invention to provide a method of preventing hair loss and preparing a composition for hair growth.

In order to solve the above problems, the present invention provides hair loss prevention function and hair growth function of a mixed extract consisting of iris, alfalfa, licorice, pine leaf, gosam, astragalus, angelica, michihyun, guava,

As a specific example, the present invention provides a composition for hair loss prevention and hair growth, which comprises, as an effective ingredient, a mixed extract consisting of iris, alfalfa, licorice, pine needle, gossam, astragalus, angelica, michihyun, guava,

In this case, the extracts of iris, alfalfa, licorice, pine needle, gossypium, cinnabar, angelica, ganoderma, guava, 0.5 to 7 parts by weight, or 0.5 to 5 parts by weight. For example, 0.5 to 5 parts by weight.

The extract is preferably an extract obtained by using water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, and most preferably a hot-water extract or an ethanol extract. In one embodiment of the present invention, a hot-water extract was used.

The composition may be provided in any one form selected from the group consisting of shampoo, hair rinse, hair tonic, hair gel, hair lotion, hair pack, hair spray, hair mousse, hair treatment and hair dye.

For this purpose, the composition for hair loss prevention and hair growth may be formulated as a coating agent, an infiltrant, an agent, a tincture agent, an extract agent, a fluid extract agent, a syrup agent, an ointment and a cream.

The present invention also provides, as another embodiment, a method for preparing a mixed extract for the preparation of a composition for hair loss prevention and hair growth comprising:

(a) 0.5 to 5 parts by weight of iris, alfalfa, licorice, pine needle, gosam, guacamole, angelica, michihyun, guava,

Extracting and separately mixing 0.5 to 5 parts by weight of iris, iris, licorice, pine needle, gosam, celestial gong, angelica, michihyun, guava,

(b) filtering the mixed extract; And

(c) concentrating the filtrate in step (b) above.

At this time, in the step (a), a hot heating mantle can be used for the hot water extraction, and the hot water extraction can be performed by heating at 80 ° C to 120 ° C for 8 to 48 hours.

Further, the filtration in step (b) may be performed using a filter paper, and the concentration in step (c) may be performed by vacuum concentration.

The present invention includes all uses that utilize the hair loss prevention function and hair growth function of a mixed extract consisting of iris, alfalfa, licorice, pine needle, gossam, astragalus, angelica, michi, guava,

The composition for hair loss prevention and hair growth according to the present invention is a natural product having no toxicity and does not cause irritation to the skin. It includes natural products which have anti-inflammatory action as well as blood circulation to hair follicle cells, There is an excellent effect on growth.

FIG. 1 is a graph showing a change in the number of hair loss of a subject to be tested by using the composition according to the present invention. In this graph, bars represent the standard deviation of the mean (n = 8) and are statistically significant difference p <0.05 at the 95% confidence level before and after application of the composition of the present invention.
FIG. 2 is a graph showing the rate of preventing hair loss after application of the composition of the present invention to a subject to be tested.
Fig. 3 is a graph showing the degree of satisfaction (n = 8) after application of the composition of the present invention to a subject to be tested.
Fig. 4 is a photograph of a subject after clinical application of the composition of the present invention.
Fig. 5 is a graph showing changes in hair density before and after application of the composition of the present invention to a subject to be tested. Bars in the graph are mean standard deviations (n = 8) and a statistically significant difference at the 95% confidence level before and after application of the composition of the present invention is p < 0.05.
Fig. 6 is a graph showing changes in hair thickness before and after application of the composition of the present invention to a subject to be tested. In the graph, bars are the standard deviation of the mean (n = 8) and are statistically significant (p < 0.05) at the 95% confidence level before and after application of the composition of the present invention.

The terms used in the present invention are defined as follows.

"Hair growth" or "hair loss prevention" is a term having similar meanings to each other and includes both the effect of promoting hair generation and hair growth in the art and preventing hair from falling or becoming weak. Particularly, the present invention has excellent hair restorative effect, but the effect of preventing hair growth and hair loss is remarkable. Prevention of hair loss (inhibition) is to suppress the growth of hair follicle cell activity by other substances. Hair loss can be prevented by improving blood flow around the hair follicles, supplying the necessary nutrients to the hair follicles to make hair and hair loss around the hair follicles around a lot of lymphocytes are used to enhance the immune function is to activate the hair follicles.

"Extract" is characterized in that it is a crude extract of iris, alfalfa, licorice, pine needle, ginseng, astragalus, angelica, michigan, guava,

The crude extract is a solvent selected from water containing purified water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, and butanol, or a mixed solvent thereof, preferably a water and methanol mixed solvent, more preferably 50 to 100% Includes extracts soluble in methanol.

The "polar solvent-soluble extract" includes extracts which are soluble in water, methanol, butanol or a solvent mixture thereof, preferably water or methanol, more preferably methanol.

"Non-polar solvent-soluble extract" includes extracts which are soluble in hexane, chloroform, dichloromethane or ethyl acetate, preferably hexane, dichloromethane or ethyl acetate, more preferably in hexane or ethyl acetate solvents.

A "control" cell, tissue, sample, or subject is the same type of cell, tissue, sample, or subject as a test cell, tissue, sample, or subject.

"Treatment" means an approach to obtaining beneficial or desired clinical results. For purposes of the present invention, beneficial or desired clinical results include, but are not limited to, alleviation of symptoms, reduction in the extent of disease, stabilization (i.e., not worsening) of the disease state, (Either partially or totally), detectable or undetected, whether or not an improvement or temporary relief or reduction "Treatment" refers to both therapeutic treatment and prophylactic or preventative measures. Such treatments include treatments required for disorders that have already occurred as well as disorders to be prevented. &Quot; Palliating " a disease may reduce the extent of the disease state and / or undesirable clinical symptoms and / or delay or slow the time course of the progression, It means to lose.

An "effective amount" is an appropriate amount that affects a beneficial or desired clinical or biochemical outcome. An effective amount may be administered one or more times. An effective amount is an amount sufficient to temporarily alleviate, ameliorate, stabilize, reverse, slow down or delay the progression of a disease state. In the present invention, it is an amount that alleviates or delays the progress of hair loss, or improves the promotion of hair growth. If the recipient animal is capable of enduring the administration of the composition, or the administration of the composition to the animal is suitable, the composition will be "pharmaceutically or physiologically acceptable ". If the dose administered is physiologically significant, it can be said that the formulation is administered in a "therapeutically effective amount ". The formulation is physiologically relevant if the presence of the formulation results in a physiologically detectable change in the recipient.

"About" means that the reference quantity, level, value, number, frequency, percentage, dimension, size, quantity, weight or length is 30, 25, 20, 25, 10, 9, 8, 7, , Level, value, number, frequency, percent, dimension, size, quantity, weight, or length that varies from one to three, two, or one percent.

Throughout this specification, the words " comprising "and" comprising ", unless the context requires otherwise, include the stated step or element, or group of steps or elements, but not to any other step or element, And that they are not excluded.

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for hair loss prevention and hair growth comprising, as an active ingredient, a mixed extract consisting of iris, alfalfa, licorice, pine needle, gossam, astragalus, angelica, michigan, guava, will be.

Specifically, in the composition of the present invention, the mixed extract is composed of iris, alfalfa, licorice, pine needle, gossam, astragalus, angelica, angelica, Guava, 0.5 to 10 parts by weight, 0.5 to 7 parts by weight, or 0.5 to 5 parts by weight based on the total weight of the composition.

If the content of each extract is less than the above-specified range, hair loss prevention effect and hair growth effect are insignificant, and if it exceeds the predetermined range of weight parts, it affects the formulation and stability, which is not preferable.

Herein, each of the weight parts represents the content ratio of the extracts, and these content ratios may not necessarily be contained in the same amount but may be contained at appropriate ranges within the above range. In addition to the above extracts, The ingredients may additionally be included.

It is preferable that the mixed extract solution contains 0.1 to 20 parts by weight per 100 parts by weight of the total composition of the present invention. When the content is less than 0.1 part by weight, hair loss prevention effect and hair growth effect / effect are weak, and when it exceeds 20 parts by weight, there is a problem in formulation stability. In one embodiment, 17.2 parts by weight of the mixed extract and 82.8 parts by weight of purified water were prepared.

In the present invention, any of the extracts commonly used in the art may be used for the iris, mallow, licorice, pine needle, gossam, celeste, angelica, mangichen, guava, (Such as methanol, ethanol, propanol, isopropanol, and butanol) having a carbon number of 1 to 4, a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform, 1,3-butylene glycol, Hexane, diethyl ether, or butyl ether. For example, by hot water extraction or ethanol extraction.

Hereinafter, each natural component contained in the composition of the present invention will be described in detail.

The iris (Acorus calamus Linn.) Is known as the sweet flag or Bacha, a perennial herb belonging to the genus Chrysanthaceae (Araceae). It is fragrant and grows in ponds, waterways and wetlands. The undergrowth has many nodes and has beard roots. The main component of essential oil, the iris extract, is asarone. Iris has been reported to treat hair loss, antibacterial, skin wound treatment, antimicrobial activity and antioxidant effect.

Root skin of Mori Cortex Radicis (Mori Cortex Radicis), tuberous, semi-oval or banded, with a thickness of 0.1 to 0.6 cm, with a white to orange-yellow outer surface and sometimes white silk-like A large number of fibers are exposed. The main ingredients are α-amyrin, kaempferol, morin, quercetin, umbelliferone, etc., and hair growth effect, whitening and moisturizing raw material, immunity control and antiallergic effect on alopecia are known.

Licorice (Glycyrrhiza uralensis) can be used as a glycyrrhiza glabra L., a G. uralensis Fisher or a root of the same plant, It will be peeled (pea licorice). Extracts include hydrocortisone receptor antagonism, anti-inflammatory action, estrogen hormone action, detoxification, hyperlipidemia, and interferon organic action of the anti-ulcerative cortex (Genetic Medicine Book, 3rd edition (2002) . The major components of flavonoids are liquiritigenin, liquiritin, licuraside, and glycyrrhizin, and anti-inflammatory effects are known.

The main ingredients of Pinus densiflora extract are cumaric acid, terpen, polyphenolic tannin and proanthocyanidie, which are known to prevent hair loss, improve skin texture and whitening, hypertension, diabetes, anticancer and antimicrobial efficacy.

Sophora Angustifolia contains various types of alkaloids and flavonoids. Its major components are d-matrine, d-oxymatrine, and has been reported to have effects on hair growth, skin aging, skin care and anti-inflammation.

Ligusticum chuanxiong Hort contains volatile essential oils and alkaloids such as dl-tetrahydropalmatine, protopine, ferulic acid, ligustilide, and butylidenpthalide, and skin whitening, antibacterial and antioxidant effects.

The major components of angelica gigas are decursin, decursinol, nodakenin, coumarin derivatives and α-pinene, limonene, β-eudesmol and elemol.

Machihyun refers to leggings, has excellent healing effect of wounds, has been widely used in skin diseases, especially as a source of skin ointment has been used. It has anti-dryness and excellent anti-inflammatory action. It has excellent effect of soothing the skin irritation and allergic reaction. It has anti-bacterial action and excellent skin moisturizing power. It also has the same effect on the scalp and is said to be good for hair loss and dandruff

Guavava (Psidum guajava) is a herbaceous plant inhabited and cultivated in tropical and subtropical regions. Its fruit and leaves have various pharmacological activities such as anti-inflammation, antibacterial, anticancer, hyperlipidemia and hypotensive activity [EP 0 154 53; Phytother. Res. 14, 388-391,2000; J. Hum. Hypertens. 7, 33-38. 1993), and it has also been known that guava has a hypoglycemic effect in model animals of type 1 diabetes (insulin dependent diabetes mellitus) induced by alloxan treatment (Am. J. Chin. Med. 11, 74-76. 1983; Shoyakugaku Zasshi. 39, 261-269, 1985].

Centella asiatica is a perennial herbaceous herbaceous perennial herb that has been known to be effective against fever, chewing gum or muscle spasms, Asian ticoside or derivatives thereof, which is a glycoside isolated from a centrum, is known to be effective for skin ulcers, extensive trauma treatment, skin veins, dermal ulcers, erosive skin ulcers, and alopecia areata. It contains very useful pharmacological ingredients such as asiaticoside, madecassoside, asiatic acid and madecassic acid, which are extracts of cloaca, And it is used as a raw material for a disease treatment agent because it is effective for anti-dementia, asthma, headache, gonorrhea, syphilis, skin disease, leprosy and gastrointestinal diseases.

It is a perennial herb that belongs to Araliaceae. Araliae Cordatae Radix (Angelica pubescens for Biserrata Shan Etyuan) is a perennial herb that belongs to Araliaceae. Its fresh roots contain 0.07% essential oil, and limonen, Alpha-cyclodextrin, sabinene, alpha-pinene, gamma-terpinene, myrcene, humulene, alpha-caryophyllene, cis and trans. It has been known to contain captiva savinen, alpha-copaene and terpenene-4-ol, and from the past the roots of virginity have been associated with gusts, hemorrhages, colds, headache, rheumatic neuralgia It has been reported to be effective. 3; Okuyama Eet al., Chem. Pharm. Bull., 39, pp 405-407, 1991) and inhibition of cyclooxygenases (COX) -1,2 (Bae KH et al. Arch. Pharm. Res., 28 (1), pp28-33, 2005) have been reported

Especially, it is known that it is used for allergic diseases, inflammatory diseases, osteoporosis diseases and the like, and it is also known as an inflammatory disease treatment and sterilization effect, but there is no known effect on hair loss prevention or hair growth.

The present invention is based on the superior hair growth and hair loss prevention function of the mixed extract.

In one embodiment, the mixed extracts were applied to eight male and female volunteers of the hair loss applicants for 10 months and the effects thereof were observed. As a result, the number of hair removed from the tester decreased with the number of days of application, And 82.67% (p <0.05), respectively. At 60 days after application, 80.24% of the testees were very satisfied with hair loss prevention. The density and thickness of hair increased significantly (128.1% and 233%, respectively) before and after application (p <0.05). The results of this one embodiment showed that the application of the natural botanical mixed extract of the present invention in hair loss has excellent effects of hair growth and prevention of hair loss.

Each extract may be contained, for example, 0.5 to 15 parts by weight, 0.5 to 13 parts by weight, 0.5 to 10 parts by weight, 0.5 to 7 parts by weight, or 0.5 to 5 parts by weight. For example, 0.5 to 5 parts by weight.

In one embodiment, 5 parts by weight of iris, 0.5 part by weight of licorice, 0.7 part by weight of licorice, 5 parts by weight of pine needle, 1.3 parts by weight of ginseng, 0.5 part by weight of ginseng, 0.7 parts by weight of Angelica gigantis, 0.6 part by weight of mallow, 0.6 parts by weight, 0.4 parts by weight as an outflow, and 0.9 parts by weight as a miscible.

Meanwhile, the composition for hair loss prevention and hair growth of the present invention can be added with a base ingredient which can be compounded in a general scalp and hair external preparations.

Specific examples thereof include solubilizers, surfactants, moisturizers, thickeners, pH adjusters, preservatives, antioxidants, sequestering agents, bactericides, anti-inflammatory agents, antimicrobial agents, solvents, coloring agents and flavoring agents.

Specifically, the dissolution aid may be isopropyl myristate, polyethylene glycol, triglycerides of medium chain fatty acids, hydrocarbons, glycols and the like.

Specific examples of the anionic surfactant in the surfactant include ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isocyanate, sodium lauryl isethionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium Lauryl ether sulfate (1 to 3 ethylene oxide), and the like. The nonionic surfactant in the surfactant may specifically be polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, hardened castor oil derivative, fatty acid diethanolamide, glyceryl stearate, and the like. The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like. The amphoteric surfactant in the surfactant may be specifically betaine, amide betaine, sulfobetaine, stear trimonium chloride, and the like

The moisturizing agent may specifically be glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, and the like.

The thickening agent may specifically be a water-soluble polymer compound such as methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxymethyl cellulose, hydroxymethyl cellulose and the like.

The pH adjuster may specifically be citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid, and the like.

Preservatives may specifically be benzoic acid, paraoxybenzoic acid esters, mixtures of methylchloroisothiazolinone, phenoxyethanol, DMDM, and the like.

The antioxidant may specifically be dibutylhydroxytoluene, ascorbic acid, and the like.

The metal ion sequestering agent may specifically be disodium ethylenediaminetetraacetate, tetra sodium ethylenediaminetetraacetate, or the like.

The bactericide may specifically be chlorhexidine gluconate, quaternary ammonium salt, pyrrothonol amine, zinc pyrithione suspension, urethropropyl butylcarbamate, salicylic acid and the like.

Anti-inflammatory agents may specifically be monoammonium glycyrrhizinate, dipotassium glycyrrhizinate, stearyl glycyrrhizinate, chamomile, alpha bisabolol, allantoin, and mixtures thereof.

Specific examples of the antimicrobial agent include phenoxyethanol, chlorhexidine, chlorhexidine gluconate, pylontonol amine, ketoconazole, arnica extract, urethropropyl butylcarbamate, benzalkonium chloride, benzenethonium chloride, benzoic acid and its salts, benzyl alcohol, lavender, , Salicylic acid, trichlorocarbane, zinc pyrithione suspensions, and mixtures thereof.

The solvent may specifically be ethanol, purified water, tween 20, cyclomethicone, mineral oil, dimethicone, and the like.

The coloring agent and the flavoring agent may be the same as those used in the conventional scalp and hair formulations.

In addition, the composition for preventing hair loss and hair growth of the present invention can be prepared with a cosmetic composition that can be applied to scalp and hair, such as liquid, cream, paste, or solid.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the herbal extract of the present invention. For example, conventional ingredients such as stabilizers, solubilizers, vitamins, Phosphorus auxiliaries and carriers

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture or the like. For example, an emulsion, a cream, a lotion, a pack, a foundation, a lotion, a serum, a hair cosmetic, and the like.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component . When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether. In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters. When the formulation of the present invention is a suspension, the carrier component may be water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl Alcohols, suspensions such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

Hair shampoo, hair rinse, hair tonic, hair gel, hair lotion, hair pack, hair spray, hair mousse, hair treatment, hair dye, hair conditioner, mixed type thereof , Mixed type of shampoo and rinse, mixed type of rinse and treatment, liquid type of hair-raising agent, etc., and also includes the aerosol type of these formulations.

The composition for hair loss prevention and hair growth is preferably used as a transdermal administration method such as directly applying or spraying on scalp or hair. The amount of the mixed extract, which is an active ingredient contained in the composition of the present invention, may vary depending on the age, symptom and method of administration. The amount of the mixed extract, which is an active ingredient contained in the composition of the present invention, is 40 mg / kg or less on an adult basis, preferably 20 mg / kg to 40 mg / kg.

In addition, the hair loss prevention and hair growth composition of the present invention may be in the form of a pharmaceutical composition.

In the case of the above pharmaceutical composition, it may be prepared by additionally containing pharmaceutically acceptable and physiologically acceptable adjuvants. Examples of the adjuvants include solvents, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, Or a solubilizing agent such as a flavoring agent can be used.

In addition to the above-mentioned effective ingredients for proper administration, one or more pharmaceutically acceptable carriers may be further formulated. Herein, the pharmaceutically acceptable carrier may be a mixture of one or more of saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, Other conventional additives such as buffers, bacteriostats and the like may be added.

In addition, the pharmaceutical composition for hair loss prevention and hair growth may be prepared by adding a diluent, a dispersant, a surfactant, a binder and a lubricant to form a parenteral, capsule, granule or tablet such as an aqueous solution, suspension or emulsion . May be administered in a conventional manner via intravenous, intraarterial, intraperitoneal, intramuscular, intraarterial, intraperitoneal, intrasternal, percutaneous, intranasal, inhalation, topical, rectal, Lt; / RTI &gt;

The pharmaceutical composition or cosmetic composition of the present invention may be prepared by mixing the herbicidal composition of the present invention with a mixture of herb extracts, alfalfa, licorice, pine needle, gossam, astragalus, Angelica gigas, And the like.

The amount of the composition of the present invention to be used can be appropriately adjusted according to individual differences such as age, degree of lesion, form, and form, and the composition of the present invention is useful as cosmetics, medicines, or quasi-drugs for improving scalp and hair condition Can be used. The content of the mixed extract as an active ingredient contained in the composition of the present invention is 40 mg / kg or less on an adult basis, preferably 20 mg / kg to 40 mg / kg.

In another aspect, the present invention relates to a method of preventing hair loss and preparing a composition for hair growth.

In another aspect, the present invention relates to a method of preventing hair loss and preparing a composition for hair growth.

In one embodiment of the method, it may be carried out by a method comprising:

(a) 0.5 to 15 parts by weight, 0.5 to 10 parts by weight, 0.5 to 10 parts by weight, 0.5 to 10 parts by weight of iris, 7 parts by weight, or 0.5 to 5 parts by weight; And

(b) heating the mixture at 80 DEG C to 120 DEG C for 8 to 48 hours to obtain a hot water extract.

As another embodiment of the method, it can be carried out by a method comprising:

(a) adding iris, alfalfa, licorice, pine needle, gossam, cinnabar, angelica, marijuana, guava, fenugreek, clover and chewiness to water, heating each mixture at 80 to 120 ° C for 8 to 48 hours Obtaining each hot-water extract; And

(b) 0.5 to 15 parts by weight, 0.5 to 10 parts by weight, 0.5 to 10 parts by weight, 0.5 to 10 parts by weight of each of the iris, mallow, licorice, pine needle, To 7 parts by weight, or from 0.5 to 5 parts by weight, and mixing to obtain a mixed extract.

As another embodiment of the method, it can be carried out by a method comprising:

(a) hot water extraction using a heating mantle by mixing iris, alfalfa, licorice, pine needle, gosam, celestial gong, angelica, michihyun, guava,

(b) filtering the extract using a filter paper; And

(c) vacuum-concentrating the filtrate in step (b) above.

Although the above method describes a method of extracting hot water by using water having a volume of about 5 to 10 times, it is obvious that a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof can be used in a suitable manner according to a known technique. The weight ratio of each of the natural products may be 0.5 to 5 parts by weight per effective ingredient, but may be contained in various proportions as needed, without limitation.

The composition for preventing hair loss and for hair growth of the present invention has an effect of improving blood circulation. Capillaries that act as nutrients to carry hair to grow hair are enlarged or reduced under the influence of autonomic nerves. When the capillaries are shrunk due to abnormal autonomic control, the nutritional supply is weakened, shortening the growth period and leading to hair loss have. Therefore, blood supply to the hair follicle is very important for hair growth, and the ginseng, astragalus and pine needle components of the present invention exert the effect of promoting the blood circulation. Thus, by supplying nutrients to the scalp membrane, the scalp of the hair loss patients is made healthier, and the metabolism is activated to prevent the roughness of the scalp, to relieve the wound and to reinforce the nutrients to the skin, Promote.

Therefore, as a result, the composition for prevention of hair loss and hair growth of the present invention stimulates hair follicle growth to promote hair growth and maintain hair roots strongly.

Example

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

This study was approved by the IACUC (KW-141027-1) of the Animal Experimental Ethics Committee of Kangwon National University.

Statistical analysis was performed using a SAS program. The mean and standard error of each treatment was calculated and analyzed by Duncan's multiple range test. The significance (p <0.05) was tested at 95% level.

Example  1: Anti-hair-loss composition Preparation, clinical trial  Selection and application of experimenter

1-1 Natural botanical extract and hair loss preventing composition containing it

(1:10 w / w) was extracted by heating mantle by mixing with the ratio suggested by NJY Biotechnology Co., Ltd. v). Specifically, 5 parts by weight of iris, 0.5 part by weight of licorice, 0.7 part by weight of licorice, 5 parts by weight of pine needle, 1.3 parts by weight of gosam, 0.5 part by weight of Angelica gigantis, 0.7 part by weight of Angelica keiskei, 0.6 part by weight of horseradish, 0.4 parts by weight of the outflow, and 0.9 parts by weight of the miscible.

In addition, the 12 natural plants listed above were mixed in the same manner as the hot water extraction, and then extracted three times with pure ethanol (1:10 w / v) for 3 hours, using a reflux extraction system.

The extract was filtered using filter paper (Whatman No. 2) and concentrated in vacuo on a rotary vacuum evaporator (EYELA, Rikakiki Co., Tokyo, Japan). After the concentration, 7.5% of hot water extract having hair growth effect was obtained and mixed with an ointment base provided by NJY Biotechnology Co., Ltd. to prepare a hair loss preventing composition. This was named as NPE (natural plant extracts).

1-2 Selection of clinical trial subjects NPE  apply

We received 12 applicants who were thought to be hair loss patients and selected eight men and women aged 20-60 years who passed out more than 100 hairs per day. NPE was applied twice a day for 10 months to prevent hair loss and to investigate hair growth effect.

Example 2: Hair loss prevention rate and applicant satisfaction

At 30, 40, and 60 days after NPE application, the hair exiting the tester was received twice in the plastic box at the same time twice, and the number was identified and presented. The number of hair loss was expressed as a percentage of the onset of application. Photographs related to hair loss and hair growth of the tester before and after application were photographed and presented. The satisfaction of the tester was evaluated by the 10 point method:

1-4 points, dissatisfied;

5-7 points satisfied;

Very satisfied with 7-10 points

The number of hairs examined at 30, 40, and 60 days after application of NPE, the effect of preventing hair loss, the satisfaction of preventing hair loss, and the results of clinical photographs are shown in FIGS. 1 to 4

The number of hair removed from the tester decreased significantly as the number of days of NPE application compared to the start date, but the percentage of hair loss prevention was significantly increased (p <0.001). As shown in Fig. 1, no hair loss occurred at a certain level until about 60 days (two months) after application.

In addition, the percentage of hair loss prevention increased to 33.33, 82.67, and 82.67% over time at 30, 40, and 60 days after application of NPE, respectively, and reached a stable level at 40 days after application (Fig. 2). In addition, 80.24% of the applicants' satisfaction with the prevention of hair loss and hair growth was very satisfactory (FIG. 3).

In addition, as shown in FIG. 4, when the state of hair before applying and the state of hair after 3 months after application were visually compared with people of various ages, hair loss prevention and hair growth promotion I was able to confirm visually that it was remarkable.

Example  3: Evaluation of hair density and thickness

In addition, the present inventors used a hair analysis system (PSI 2003, Hair and Sclap Microscope System, SIF-1, Korea) at 30, 40, and 60 days after NPE application to measure the density and thickness Were measured. The density of the hair was 0.6 mm ² The number of hairs per hair was measured and the thickness was measured with the program input to the instrument.

As a result, dark and durable hair began to grow from day 20 after NPE application, and the density and thickness changes of hair measured at 30, 40, and 60 days intervals are shown in FIG. 5 and FIG.

The density and thickness of hair increased significantly after 30, 40, and 60 days of application (p <0.001). The hair densities at 30, 40 and 60 days were increased to 62.92, 97.88 and 128.1%, respectively (Fig. 5), and the hair thickness was also increased by 133, 233 and 233% (Fig. That is, it was confirmed that the hair growth effect of the NPE of the present invention is also excellent through the density and thickness of the hair.

On the other hand, when the hair was pulled by tweezers after 30 days, it was found that it was hardly pulled out. As a result, it was found that application of NPE keeps the root firmly.

From these results, it was confirmed that application of the natural plant-derived mixed extract (NPE) as a composition of the present invention strengthens the density and thickness of the hair by firmly keeping the hair roots, and thus has the effect of preventing hair growth and hair loss .

Claims (10)

5 parts by weight of iris, 0.5 parts by weight of licorice, 0.7 parts by weight of licorice, 5 parts by weight of pine needle, 1.3 parts by weight of gosam, 0.5 part by weight of Angelica gigantis, 0.7 part by weight of Angelica gigantis, 0.6 part by weight of mallow, A composition for preventing hair loss and hair growth, which comprises an extract comprising, as an active ingredient, an extract comprising 0.6 part by weight of a herbal composition, 0.4 part by weight of an extract, and 0.9 part by weight of a chewing gum.
delete delete delete The method according to claim 1,
Wherein the composition is any one selected from the group consisting of shampoos, hair rinses, hair tonics, hair gels, hair lotions, hair packs, hair sprays, hair mousses, hair treatments and hair dyeing agents. Composition.
The hair growth preventing and hair growth method according to claim 1, wherein the composition for hair loss prevention and hair growth is formulated as a coating agent, an infiltrant, an agent, a tincture agent, an extract agent, a fluid extract agent, a syrup agent, / RTI &gt;
A method for preparing a blended extract for hair loss prevention and hair growth composition comprising:
(a) 5 parts by weight of iris, 0.5 part by weight of licorice, 0.7 part by weight of licorice, 5 parts by weight of pine needle, 1.3 parts by weight of gosam, 0.5 part by weight of Angelica gigantis, 0.7 parts by weight of Angelica gigantis, 0.6 part by weight of mallow, 0.4 part by weight of the outflow and 0.9 part by weight of the untoward water, followed by hot water extraction or separately hot water extraction and mixing;
(b) filtering the mixed extract; And
(c) concentrating the filtrate in step (b) above.
The method according to claim 7, wherein in the step (a), the heating water is extracted using a heating mantle.
The method according to claim 7, wherein the hot water extraction in step (a) is performed by heating at 80 ° C to 120 ° C for 8 to 48 hours.
The method according to claim 7, wherein the concentration of step (c) is vacuum concentration.

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