KR101698922B1 - Composition for promoting hair growth and restoration - Google Patents

Abstract

Since the composition for promoting hair growth and hair growth according to the present invention contains ginsenosides Rb2, Rc and Rg1 having increased contents, hair growth and hair growth promoting effect can be exhibited more than the conventional hair growth promoting agent.
In addition, since the plant natural material is used, it can be used as an external preparation for skin for promoting hair growth and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions and food compositions.

Description

TECHNICAL FIELD The present invention relates to a composition for promoting hair growth and hair growth,

The present disclosure relates to a ginseng extract having an increased content of ginsenoside and a composition for promoting hair growth and hair growth promoting growth of hair by shortening the period of transition from a resting period to a growing period.

Along with the recent increase in social stress, the hair loss population is gradually increasing due to westernized eating habits such as environmental pollution and instant food, frequent perm and dyeing. The cycle of hair consists of anagen which grows hair, catagen which is the period of termination of growth and reduction of the size of the moles, taloge which is the period when the papilla stops its activity and keeps its hair on the scalp, Or a generator that generates new hair to depilate old hair.

The Anagen Stage (2-7 years) is the period of hair growth, divided into hair growth stages where the hair is going to go out from the hair to the blanket and the stage where the hard keratin is made inside the hair follicle. Hair continues to self-grow until the retrogressive. Catagen Stage (2 to 3 weeks) is the period when the metabolic process is slowed down at the end of the growing season, maintaining the shape of the hair, and does not produce keratin at this stage. Degenerators account for 1% of total hair. At this time, the mothers shrink and divide into the dermal papilla, surrounded by the hair follicles and go upwards, and the cell division is stopped. The Talogen Stage (3 months) shrinks the papillary, the hair follicles shrink gradually, and the hair follicles are pushed upwards and fall out. The lifespan of the hair until the next stage of growth begins is 3 to 4 months.

A person with normal hair growth has a lot of hair, but a person with alopecia (Alopecia) has a lot of hair in a resting state, so that it shows visible hair loss phenomenon. As hair loss progresses, the period of growth is shortened and hair becomes smaller and smaller. Therefore, in order to treat hair loss, it is important to allow the hair follicle in a resting state to rapidly grow and to shorten the growth period.

Male alopecia are caused by testosterone, a testosterone that is converted to a more powerful hormone, Dihydrotestosterone (DHT) by an enzyme called 5-alpha-reductase. Hormone acts on the hair follicle to induce the hair follicle from the growing stage to the regenerator stage to cause hair loss. Therefore, a method of inhibiting the production of DHT by 5 alpha-reductase in order to treat male alopecia is mainly used.

Female alopecia are caused mainly by a decrease in the amount of estrogen after menopause. Minoxidil and estrogen are mainly used as therapeutic agents for female alopecia.

Alopecia areata is caused by autoimmune diseases, mental stress, genetic predisposition. These alopecia areata are fundamentally different from those of androgenetic alopecia, and different methods of treatment are used, either by a method of treating corticosteroids, or by applying minoxidil to the affected area or artificially inducing a stimulus to the affected area.

As a result, it has been known that there are no known effects on hair loss caused by various complications, such as blood circulation promotion, suppression of male hormone function, hair follicle enhancement, etc. However, . For example, minoxidil has been reported to cause sticky sensation and side effects that cause irritation to the skin. Pinastaride is currently used as a preparation for oral administration, but adverse effects such as sexual dysfunction have been reported depending on its consumption, There was a lot of inconvenience to use because it was expected to be effective only by oral administration.

Korean Patent Publication No. 10-2011-0000433

In order to solve the above-mentioned problems, the present invention provides a ginseng extract having increased ginsenoside content and promoting hair growth and hair growth promoting hair growth by shortening the period of transition from a resting period to a growing period, And it is an object of the present invention to provide a composition for use in the present invention.

In order to solve the above-mentioned problems,

An embodiment of the present invention provides a ginseng extract comprising 2.5% by weight or more of ginsenoside Rb2, 3% by weight or more of ginsenoside Rc, and 2% by weight or more of ginsenoside Rg1 based on the total weight of ginseng extract .

Also, an embodiment of the present invention provides a ginseng extract comprising 6% by weight or more of ginsenoside Re in the ginseng extract.

Another embodiment of the present invention is a method for producing the ginseng extract,

Adding water, an organic solvent or a mixture of water and an organic solvent to the ginseng, extracting the ginseng while repeating the pressurization and decompression extraction steps in sequence;

The ginseng extract obtained by the above step is dissolved in water and extracted with an organic solvent to remove the organic solvent layer and the water layer is further extracted with an organic solvent to prepare a ginseng extract. .

Another embodiment of the present invention provides a composition for promoting hair growth and hair growth comprising the ginseng extract having an increased content of ginsenoside.

The composition for promoting hair growth and hair growth according to the present invention contains a large amount of ginsenosides Rb2, Rc and Rg1 or Rb2, Rc, Rg1 and Re at an increased concentration, so that the hair growth promoting effect have.

In addition, since the plant natural material is used, it can be used as an external preparation for skin for promoting hair growth and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions and food compositions.

FIG. 1 is a structural formula showing chemical structures of each type of ginsenoside according to an embodiment of the present invention.
FIG. 2 is a graph of HPLC analysis of ginsenosides contained in ginseng extract according to an embodiment of the present invention.
3 is a graph showing the HPLC analysis of ginsenoside contained in the general ginseng extract of the comparative example of the present invention.
FIG. 4 is a graph showing an increase in expression of hair growth factor VEGF in the dermal papilla cells of ginseng extract (Example 1) according to one embodiment of the present invention and general ginseng extract (Comparative Example 1) of Comparative Example.
FIG. 5 is a graph showing the hMPc proliferation effect of ginseng extract (Example 1) according to one embodiment of the present invention and general ginseng extract (Comparative Example 1) of Comparative Example.
FIG. 6 is a graph showing the hDPc proliferation effect of the ginsenosides according to the ginseng extract, which is a major component of the ginseng extract according to an embodiment of the present invention.
FIG. 7 is a graph showing the hair growth promoting effect of the ginseng extract (Example 1) according to one embodiment of the present invention and the general ginseng extract (Comparative Example 1) of Comparative Example in a human hair follicular organ.
FIG. 8 is a graph showing the effect of ginseng extract (Example 1) according to an embodiment of the present invention, general ginseng extract of Comparative Example (Comparative Example 1), ginsenoside Rb1, Rb2, Rc, Rd and Realc Rg1 This figure shows the hair growth promoting effect.

Hereinafter, the present invention will be described in detail.

An embodiment of the present invention provides a ginseng extract comprising 2.5% by weight or more of ginsenoside Rb2, 3% by weight or more of ginsenoside Rc, and 2% by weight or more of ginsenoside Rg1 based on the total weight of ginseng extract .

An embodiment of the present invention provides a ginseng extract comprising 6% by weight or more of ginsenoside Re for the ginseng extract.

Ginsenoside is a type of glycoside saponin derived from the roots, stems, leaves, bark and seeds of plants. There are various kinds of ginsenosides such as ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1 and the like. There are various kinds of ginsenosides such as ginsenosides The kinds and contents of senosides are different. Figure 1 shows the chemical structure of each ginsenoside.

Rb1, Rb2, Rc, Rd, Re and Rg1 can be divided into PPT (Re, Rg1) and PPD (Rb1, Rb2, Rc and Rd) It is water-soluble as a polar substance which has not undergone denaturation by removing sugars through oxidation.

Another embodiment of the present invention provides a ginseng extract obtained from roots of a ginseng region by the above ginseng extract. Specifically, there is provided a ginseng extract obtained from a ginseng root (ginseng) having a high ginsenoside yield.

In one embodiment of the present invention, each of the ginsenosides may include 2.5% by weight or more of ginsenoside Rb2 relative to the total weight of ginseng extract, 3% by weight or more of ginsenoside Rc, Re may be contained in an amount of 6 wt% or more, and ginsenoside Rg1 may be contained in an amount of 2 wt% or more. 2.5 to 4.5% by weight of ginsenoside Rb2, 3 to 7.5% by weight of Rc, 6 to 20% by weight of Re and 2 to 5% by weight of Rg1.

According to an embodiment of the present invention, the ginseng extract comprises at least 2.5% by weight of ginsenoside Rb2, at least 3% by weight of Rc, at least 6% by weight of Re and at least 2% by weight of Rg1, The ginseng extract can be produced by using any method that can extract components such as ginsenoside from ginseng without limitation. For example, a pressure-circulating type extraction process or a solvent fractionation process in which a pressurization process or a depressurization process is alternately carried out, or they may be continuously carried out. At this time, the pressure circulation type extraction process can be specifically performed by adding water, an organic solvent or a mixture thereof to the ginseng and circulating the pressure and the reduced pressure at regular intervals.

More specifically, the present invention provides a ginseng extract having the above-mentioned contents of high ginsenosides Rb2, Rc and Rg1 as an example, by the following method.

Adding water, an organic solvent or a mixture of water and an organic solvent to the ginseng, extracting the ginseng while circulating the pressurized and reduced-pressure extraction steps sequentially, and

The ginseng extract may be prepared by dissolving the ginseng extract obtained in the above step in water, extracting it with an organic solvent, removing the organic solvent layer, and then extracting the water layer again with an organic solvent.

Specifically, the type of the solvent that can be used as one embodiment of the present invention is not particularly limited, and examples thereof include water, an organic solvent, or a mixture of water and an organic solvent. For example, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform. For example, the mixed solvent of water and organic solvent may have an organic solvent ratio of 10 to 90% (v / v). More specifically, the solvent may be an extract extracted using 10 to 90% (v / v) ethanol, more specifically 70% (v / v) ethanol.

According to an embodiment of the present invention, the pressurization and decompression extraction process may be performed for 1.5 to 2.5 hours while circulating the pressurization and decompression extraction process for 20 to 40 minutes sequentially by using a pressure circulation type extraction device . The pressurization pressure is 1 to 3 kgf / cm ² , the pressure during the decompression extraction is 550 to 650 mmHg, and the extraction temperature is 65 to 85 ° C according to an embodiment of the present invention.

When the depressurization and pressurization cyclic processes as described above are carried out, the thermal denaturation of ginseng can be minimized, and the ginsenoside denaturation, for example, the fact that sugar chains from ginsenoside Rb1 to Rg3 can be minimized. Therefore, the extraction yield can be improved by 25 ~ 30% compared to the extraction without repeated use of the conventional simple extracting device of ginseng extract. After that, the content of ginsenoside can be maximized by removing the non-polar substance using an organic solvent such as ethyl acetate and extracting the water layer with an organic solvent such as butanol. Specifically, according to such a solvent fractionation method, the total content of ginsenosides in ginseng extract is 10 times or more, more specifically 15-25 By weight.

Since the composition for promoting hair growth and hair growth comprising the ginseng extract as described above can effectively shorten the period of transition from the resting period to the growing period in the hair growing period, The hair growth promoting effect is remarkably superior to that of the case. Therefore, it is possible to achieve an effect of promoting excellent hair growth and hair growth which have not been existed.

According to one embodiment, the present invention provides the use of the ginseng extract described in the specification of the present invention for promoting hair growth and hair growth, or for promoting hair growth and hair growth of the composition containing the ginseng extract.

Another embodiment of the present invention provides a method for promoting hair growth and hair growth comprising administering an effective amount of the ginseng extract described in the specification of the present invention to an object as described above, ≪ / RTI >

Further, another embodiment of the present invention may provide the ginseng extract described in the specification of the present invention as described above for use in promoting hair growth and hair growth, wherein the ginseng extract may be used as a composition.

According to one embodiment of the present invention, the amount of the ginseng extract containing the ginsenosides Rb2, Rc and Rg1 is not particularly limited, but may be, for example, 2% by weight or more based on the total weight of the composition , For example from 2 to 20% by weight. When the ginseng extract is contained in an amount of less than 2% by weight, sufficient hair growth effect can not be expected. If it exceeds 20% by weight, safety or formulation is difficult to produce.

The composition according to one embodiment of the present invention may be, for example, a pharmaceutical composition, a cosmetic composition or a health food composition.

The pharmaceutical composition for promoting hair growth and hair growth according to an embodiment of the present invention may be added with preservatives, stabilizers, wetting agents or emulsifying accelerators, pharmaceutical adjuvants such as salts and / or buffers for controlling osmotic pressure and other therapeutically useful substances And may be formulated into various oral or parenteral dosage forms according to conventional methods.

Examples of the oral administration agent include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, powders, powders, granules, granules and pellets, , Diluents (such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (such as silica, talc, stearic acid and magnesium or calcium salts thereof and polyethylene glycols) . The tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. The tablets may be prepared by conventional mixing, granulating or coating methods. In addition, it may be a transdermal dosage form in the form of parenteral administration. For example, it may be in the form of injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, But is not limited thereto.

The pharmaceutical composition according to one embodiment of the present invention may be administered by parenteral, rectal, topical, transdermal, subcutaneous, and the like. The pharmaceutical composition according to one embodiment of the present invention may be administered topically, for example, to the scalp.

The dosage of the active ingredient is within the level of those skilled in the art, and the daily dose of the drug depends on various factors such as the degree of progress of the subject to be administered, the age of onset, age, health condition, As a standard, it is generally possible to administer the composition at a dose of 1 / / kg to 200 mg / kg, preferably 50 / / kg to 50 mg / kg, once or twice a day, Are not intended to limit the scope of the present invention.

The composition for promoting hair growth and hair growth according to an embodiment of the present invention may be a cosmetic composition, and the cosmetic composition may contain a cosmetically or dermatologically acceptable medium or base. It may be in any form suitable for topical application, for example, as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing the oil phase in water, a suspension, a microemulsion, a microcapsule, In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. These compositions may be prepared according to conventional methods in the art. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, and examples thereof include softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, Cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil and body essence.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

According to one embodiment of the present invention, the content of the active ingredient is not particularly limited, but an extract containing the active ingredient may be contained in an amount of 0.001 to 20% by weight based on the total weight of the composition. When the above content is satisfied, excellent efficacy can be exhibited without side effects.

The cosmetic composition according to an embodiment of the present invention may further include components included in functional additives and general cosmetic compositions in addition to the extract containing the active ingredient. The functional additives may include water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts.

The cosmetic composition of the present invention may further contain, in addition to the above-described functional additive, components contained in a general cosmetic composition as required. Examples of the other ingredients that can be included in the composition include humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbents, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, Accelerators, coolants, antiperspirants, purified water, and the like.

In addition, the composition for promoting hair growth and hair growth according to an embodiment of the present invention is an external preparation for skin, and the external preparation for skin may be any kind applied to the outside of the skin, and various cosmetic formulations and medicines may be included therein have.

In addition, the composition for promoting hair growth and hair growth according to an embodiment of the present invention may be a health food composition, and the health food composition of the present invention may comprise ginsenoside Rb2, Rc, Re, Rg1, By weight based on the total weight of the composition. When the extract is less than 0.001% by weight, the effect is not sufficiently exhibited. When the extract is more than 20% by weight, the efficiency of the addition of the active ingredient decreases.

In the health food composition according to an embodiment of the present invention, the composition may be in a liquid or solid form and may be in the form of a tablet, a capsule, a soft capsule, a pill, a granule, a drink (drink) Caramel formulation or confectionery formulation, and the formulation is not particularly limited. In the health food composition of the present invention, the health food composition may contain excipients, saccharides, fragrances, pigments, oils, proteins and the like as needed in addition to the active ingredients.

Hereinafter, the present invention will be described with reference to the following examples and test examples. The examples and the test examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to the range of the following examples.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the scope of the invention.

[Example 1] Preparation of ginseng extract containing increased amounts of ginsenoside Rb2, Rc, Rg1 and Re

Ginseng (Geumsan ginseng, purchased from: Geumsan ginseng Nonghyup) was washed with purified water, dried, and pulverized to obtain ginseng powder which had been hyaluronized. 20 g of 50% ethanol was added to 2 g of the obtained ginseng powder, and the mixture was continuously subjected to a pressure and decompression extraction process with a pressure circulation type extracting device for 30 minutes, respectively, for a total of 2 hours. At this time, the pressure during the pressure extraction was 2 kgf / cm ² , the pressure during the decompression extraction was 600 ± 50 mmHg, and the extraction temperature was set at 75 ° C. The ginseng extract obtained by the above process was filtered, and the supernatant was dried under reduced pressure to obtain a dry weight of 0.57 g. Then, the dried material was dissolved in water and extracted with ethyl acetate to remove the ethyl acetate layer. The water layer was extracted with butanol and dried under reduced pressure to obtain 2.7 g of dry weight. This was set as Example 1.

In addition, the ginsenosides contained in the ginseng extract of Example 1 were subjected to HPLC analysis under the following analytical instruments and conditions, and the results are shown in a graph. From these results, the content of each component of ginsenoside contained in the extract was analyzed and shown in Table 2 and FIG. 2 in terms of% by weight.

HPLC Analytical instruments and analytical conditions

Device: Waters 2998 PDA detecter, 1525 pump, 2707 autosampler

Column: Sun fire C18 305um, 4.6 X150 mm

Detector: UV 203nm

Flow rate: 1 mL / min

Injection volume: 20ul

Conditions: gradient (A-water, B-acetonitrile)

Time (min) A (water) B (acetonitrile) 0 82 18 22 82 18 32 70 30 60 50 50

division content(%) Rg1 Re Rb1 Rc Rb2 Rd Ginseng extract (Example 1) 2.1 6.23 3.52 3.14 2.91 1.58

As a result, as shown in Table 2 and FIG. 2, Example 1 of the present invention has 2.91% by weight of ginsenoside Rb2 of 2.5% by weight or more, 3.14% by weight of Rc of 3% by weight or more, 6.23% by weight of Re of 6% % And Rg1 of 2.1 wt% or more, which is 2.0 wt% or more.

[Comparative Example 1] Preparation of general ginseng extract

Ginseng (Geumsan ginseng, purchased from: Geumsan ginseng Nonghyup) was washed with purified water, dried, and pulverized to obtain ginseng powder which had been hyaluronized. 2 g of the obtained ginseng powder was extracted with 100 ml of 50% ethanol at 75 ° C for 2 hours, and then the sample was filtered, and the supernatant was dried under reduced pressure to obtain a dry weight of 0.30 g. This was regarded as Comparative Example 1.

In addition, the ginsenosides contained in the ginseng extract of Comparative Example 1 were subjected to HPLC analysis under the same analytical instrument and conditions as in Example 1, and the results are shown in a graph. From these results, the content of each component of ginsenoside contained in the extract was analyzed and shown in Table 3 and FIG. 3 in terms of% by weight.

division content(%) Rg1 Re Rb1 Rc Rb2 Rd Ginseng extract (Comparative Example 1) 0.14 0.16 0.67 0.55 0.33 0.13

As a result, as shown in Table 3 and FIG. 3, Comparative Example 1 contained 0.33 wt% of ginsenoside Rb2, 0.55 of Rc, 0.16 wt% of Re, and 0.14 wt% of Rg1, 1 contained about 5 to 15 times or more of each ginsenoside than the general ginseng extract. In addition, it can be confirmed that the ginsenoside contained in the ginseng extract had a significantly higher total weight than Example 1 of the present invention.

[Test Example 1] Evaluation of expression of hair growth factor VEGF in dermal papilla cells

The in vitro system was applied to evaluate the degree of expression of vascular endothelial growth factor (VEGF), a growth factor necessary for movement of the ginseng extract to the growth phase of hair as a growth factor necessary for angiogenesis After the application of ginseng extract, the activity was evaluated.

For this experiment, 4 x 10 ⁵ cells of a 47-year-old male Dermal Papilla Cell (DPC) (P.11) were seeded in a 12-well plate and cultured in a 10% fetal bovine serum ) In DMEM (Dulbecco's modified Eagle's medium) overnight. After culturing, Example 1 and Comparative Example 1 were treated with 20 ppm, respectively. As a negative control, DMSO was used. After 24 hours, the treated mixture of Example 1 or Comparative Example 1 was collected (soup collected), and the amount of the ginseng of Example 1 and Comparative Example 1 was measured using a VEGF ELISA (Vascular Endothelial Growth Factor Enzyme-Linked Immunosorbent Assay; R & D biosystems) And the difference in expression level according to the extract was confirmed. The results are shown in Fig.

As shown in FIG. 4, the ginseng extract, Comparative Example 1, showed no significant difference in the effect compared to the control group without the ginseng extract. However, Example 1 of the present invention increased expression of hair growth factor VEGF more than three times in the dermal papilla cells when used at the same concentration as in Comparative Example 1. This is because the ginseng extract of Example 1 contained more ginsenosides Rb2, Rc and Rg1 than the general ginseng extract of Comparative Example 1, thereby more effectively increasing the expression of hair growth factor VEGF in the dermal papilla cells. Therefore, the ginseng extract according to the present invention has excellent hair growth promoting ability.

[Test Example 2] Evaluation of proliferation effect of dermal papilla cells

The keratin proteins that make up the hair are produced in hair follicle keratinocyte, which keratinocytes differentiate from the dermal papilla cells. Therefore, if the ginseng extract according to the present invention promotes the activity of the dermal papilla cells, the activity of the keratin-forming cells differentiated therefrom can be promoted, and furthermore, the hair growth can be promoted.

In this experiment, the promoting effect of ginseng extract on dermal papilla cell activity was evaluated as follows.

First, a human dermal papilla cell line was used in the present invention. The cell line was cultured in an incubator maintained at 37 ° C in 5% CO ₂ with Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gaithersburg, MD, USA) containing 10% bovine serum serum The ginseng extract of Example 1 was treated with 10 ppm and 20 ppm, and the ginseng extract of Comparative Example 1 was treated with 20 ppm. As a negative control, DMSO was used. Cell proliferation (%) was measured using a WST-1 kit (Roche) 24 hours after treatment of the test substance. The results are shown in Fig.

As shown in FIG. 5, the ginseng extract of Example 1 significantly increased the proliferation of both cells at 10 ppm and 20 ppm. In particular, the treatment with 10 ppm of Example 1 showed a growth rate of the papillary cell proliferation of about 1.5 times higher than that of Comparative Example 1 with 20 ppm of the common ginseng extract. This means that the ginseng extract according to the present invention can promote the proliferation of the papillary cells more than the conventional ginseng extract, and thus it is possible to more effectively promote the keratin-forming cell activity and hair generation.

[Test Example 3] Evaluation of hair growth promoting effect using human hair follicular organ

Experiments were conducted to confirm the hair growth promoting ability in the actual human hair follicle organ of the present invention.

First, ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1, which are major constituents of ginseng extract, were treated with 0.1 μM and 1 μM of dermal papilla cells, respectively, in the same manner as in Test Example 2, Cell proliferation (%) was measured using WST-1 kit (Roche). The results are shown in Fig.

Then, the hair follicular organ was separated from the occipital scalp tissue of a 55 year-old male, and the hair follicle was divided into William 'E medium (L-glutamine (2 mM), insulin (10 μg / ml), hydrocortisone (40 ng / ml) , And antifungal agent (1%)). The hair follicles grown on the third day after culturing were selected and cut into 3 mm, and the hair follicle tissues selected were treated as a control group and the experimental group was treated with 2 ppm and 5 ppm of the ginseng extract obtained in Example 1 And 5 ppm of Comparative Example 1, respectively. After 8 days from the treatment, length measurement and photographing were carried out. In the same manner as above, 5ppm of each of ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Comparative Example 1, The results are shown in Fig. 7 and Fig.

As a result, as shown in FIG. 6, ginsenosides Rb1, Rc, and Rg1 in the ginsenosides showed pronounced hair growth promoting effect. This is all statistically significant.

As shown in FIG. 7, the hair growth promoting effect of the ginseng extract treated with 5 ppm of the hair length in the hair follicular organ was superior to that of Comparative Example 1 and the control group treated at the same concentration. Especially, in comparison 1, average growth length was decreased than control. In FIG. 8, the average growth length of the ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 increased in comparison with Comparative Example 1,

6, 7, and 8, the ginseng extract according to the present invention has a higher content of ginsenosides Rb1, Rc, and Rg1 having hair growth promoting effect than the conventional ginseng extract It can be seen that the effect of promoting hair growth in the hair follicle is excellent.

[Test Example 4] Clinical trial for patients with hair loss

The control group except for the scalp essence containing 2% of Example 1 and the Example 1 component were divided into 23 test groups (Example 1) and control group , And applied to the hair loss site once a day on the evening of the day. The application period was 16 weeks. The hair growth effect was evaluated by subjective assessment of the subjects, evaluation of the researchers by clinical photographs, and hair density, hair thickness, hair growth rate using phototrichogram which is a noninvasive method of objective hair measurement, The hair loss prevention and hair growth effect were evaluated by measuring the biological parameters of the hair including the number of dropped hair. This study consisted of randomized, double-blind, active-controlled clinical trials. The experimental results are shown in Tables 4 to 8 below.

The hair densities of Example 1 and the control group were compared in Table 4. In the case of Example 1, 5.485% and 7.409% were increased after 8 weeks and 16 weeks, respectively, and significant changes were observed at all time points. The improved proportion of subjects was 86.364% and 86.364% after 8 weeks and 16 weeks, respectively. On the other hand, the control group increased 1.615% and 1.334% after 8 and 16 weeks, respectively.

The hair growth rates of Example 1 and the control group were compared in Table 4. In Example 1, the increase was 3.218% and 8.783% after 8 weeks and 16 weeks, respectively, and significant changes were observed after 16 weeks. The percentage of improved subjects was 68.182% and 81.818% for 8 weeks and 16 weeks, respectively. In the control group, they increased 2.480% and 1.142% after 8 and 16 weeks, respectively.

MEAN ± STDEV Improvement rate (%) Improved subject
ratio(%) Before use  prepare Significant difference  (p value ) Significant difference to control group
(p value ) Example Before use 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks density
(n / cm2 ) 111.045 ± 20.285 117.136 ± 19.833 119.273 ± 19.452 5.485 7.409 86.364 86.364 0.000 * 0.000 * 0.008 # 0.001 # Growth rate
(탆 / day ) 0.292 ± 0.056 0.301 + 0.080 0.318 + 0.044 3.218 8.783 68.182 81.818 0.098 0.004 * 0.496 0.025 ** Control group Before use 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks density
(n / cm2 ) 123.826 ± 18.968 125.826 ± 23.796 125.478 ± 25.293 1.615 1.334 47.826 43.478 0.267 0.444 N.A N.A Growth rate
(탆 / day ) 0.289 + 0.047 0.297 + 0.042 0.286 + 0.046 2.480 -1.142 50,000 45.455 0.471 0.732 N.A N.A

*: Paired samples t-test, p < 0.05

**: Independent samples t-test, p < 0.05

#: Mann-Whitney U test, p <0.05

The hair thicknesses of Example 1 and the control group were compared in Table 5. In the case of Example 1, 1.526%, 2.611%, 4.782%, and 7.178% increased after 2 weeks, 4 weeks, 8 weeks, and 16 weeks, respectively. The percentage of improved subjects was 72.727%, 81.818%, 95.455%, and 95.455% after 2 weeks, 4 weeks, 8 weeks, and 16 weeks of use, respectively. In the control group, 0.903%, 0.765%, 0.595% and 1.084% decreased after 2, 4, 8 and 16 weeks, respectively.

MEAN ± STDEV Improvement rate (%) Improved subject
ratio(%) Before use  prepare Significant difference  (p value ) Contrast to control
Significant difference
(p value)
Thickness (㎛) Example Control group Example Control group Example Control group Example Control group Before use 0.080 0.010 0.082 0.011 N.A N.A N.A N.A N.A N.A N.A after 2 weeks 0.082 0.011 0.081 0.011 1.526 -0.903 72.727 34.783 0.021 † 0.084 0.002 # After 4 weeks 0.083 0.011 0.081 0.010 2.611 -0.765 81.818 52.174 0.001 † 0.235 0.001 # After 8 weeks 0.084 0.012 0.081 0.011 4.782 -0.595 95.455 43.478 0.000 † 0.134 0.000 # After 16 weeks 0.086 0.012 0.081 0.010 7.178 -1.084 95.455 52.174 0.000 * 0.218 0.000 #

*: Paired samples t-test, p < 0.05

†: Wilcoxon signed rank test, p <0.05

#: Mann-Whitney U test, p <0.05

The number of dropped hairs in Example 1 and the control group was compared in Table 6. In Example 1, there was a 1.589% increase after 4 weeks of use, a 4.849% decrease after 8 weeks, a 33.278% decrease after 16 weeks and a significant change after 16 weeks. The improved proportion of subjects was 50.000%, 54.545%, and 72.727% after 4, 8 and 16 weeks of use, respectively. In the control group, the increase was 26.721% after 4 weeks, 1.953% after 8 weeks, and 2.364% after 16 weeks.

MEAN ± STDEV
Improvement rate (%) Improved subject
ratio(%)
Before use  prepare Significant difference  (p value ) Contrast to control
Significant difference
(p value ) Number of missing hair
( ea ) Example Control group Example Control group Example Control group Example Control group Before use 54.364 ± 29.589 42.304 ± 20.328 N.A N.A N.A N.A N.A N.A N.A After 4 weeks 55.227 ± 28.846 53.609 ± 38.577 1.589 26.721 50,000 43.478 0.903 0.102 0.452 After 8 weeks 51.727 ± 36.482 41.478 ± 25.472 -4.849 -1.953 54.545 43.478 0.733 0.870 0.776 After 16 weeks 36.273 ± 23.511 43.304 ± 34.189 -33.278 2.364 72.727 52.174 0.006 * 0.833 0.047 **

*: Paired samples t-test, p < 0.05

**: Independent samples t-test, p < 0.05

The deterioration improvement and visual evaluation of Example 1 and the control group were compared in Table 7. In the case of Example 1, 0.045 points, 0.318 points and 0.364 points were increased after 4 weeks, 8 weeks and 16 weeks, respectively, and significant changes were observed at 8 weeks and 16 weeks after use. The improved proportion of subjects was 9.091%, 22.727%, and 36.364% after 4, 8, and 16 weeks of use, respectively. In the control group, 0.130 point decrease, 0.087 point decrease, and 0.087 point decrease after 4 weeks, 8 weeks, and 16 weeks, respectively. The improved proportion of subjects was 0.000%, 17.391%, and 17.39% after 4, 8, and 16 weeks of use, respectively.

MEAN ± STDEV Improved Subject Ratio (%) Before use  prepare Significant difference  (p value ) Contrast to control
Significant difference
(p value ) Hair loss improvement
( Score ) Example Control group Example Control group Example Control group Before use 0.000 占 .000 0.000 占 .000 N.A N.A N.A N.A N.A After 4 weeks 0.045 ± 0.375 -0.130 + 0.344 9.091 0.000 0.564 0.083 0.110 After 8 weeks 0.318 ± 0.646 0.087 ± 0.515 22.727 17.391 0.038 † 0.414 0.297 After 16 weeks 0.364 + 0.790 -0.087 ± 0.848 36.364 17.391 0.046 † 0.627 0.070

†: Wilcoxon signed rank test, p <0.05

(3), better (2), slightly better (1), no change (0), slightly worse (-1), worse (-2) and worse (-3)

※ Since the value before use was 0, the improvement rate could not be obtained.

The subject subjective improvement ratings of Example 1 and the control group were compared in Table 8. In the case of Example 1, 40.9% and 63.6% were positive, respectively, at 8 weeks and 16 weeks, respectively, and only 0.0% and 4.4% were negative at 8 weeks and 16 weeks, respectively. In the control group, 52.1% and 39.1% were positive after 8 and 16 weeks, respectively. The negative values were 0.0% and 8.7% at 8 and 16 weeks, respectively.

MEAN ± STDEV Affirmative response rate (%) False Answers (%) Significant difference (p value ) Example 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks Hair loss improvement
( Score ) 2.455 ± 0.596 2.727 ± 0.767 40.909 63.636 0.000 4.545 0.486 0.087 Control group 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks 8 weeks 16 weeks Hair loss improvement
( Score ) 2.565 ± 0.590 2.348. + -. 0.714 52.174 39.130 0.000 8.696 N.A N.A

* Subject subjective improvement rating: Very good (4), Good (3), Average (2), Poor (1), Very poor (0)

※ Positive response rate (%): Percentage of subjects who answered "Very good" (4), "Good" (3)

※ Rate of negative answer (%): Percentage of subjects who answered "poor" (1) or "very bad" (0)

In conclusion, Example 1 of the present invention showed excellent hair loss prevention and hair growth effect compared with the control group by applying once a day as a result of effectiveness evaluation.

Formulation examples of the composition according to the present invention will be described below, but the present invention can be applied to various other formulations, which are not intended to limit the present invention but to describe it specifically.

[Formulation Example 1] Shampoo for external skin application

Shampoos were prepared in a conventional manner according to the composition shown in Table 9 below.

ingredient Content (% by weight) The extract of Example 1 2.00 Sodium lauryl sulfate 10.00 Cocamidopropyl betaine 3.00 Carboxyl vinyl polymer 0.30 Polyquaternium-10 0.20 Cetyltrimethylammonium chloride 0.10 Purified water Balance Sum 100.00

[Formulation Example 2] Rinse in external preparation for skin

Rinse was prepared according to a conventional method according to the composition shown in Table 10 below.

ingredient Content (% by weight) The extract of Example 1 2.00 Cetyl alcohol 2.00 Stearyl alcohol 2.50 Behenyl alcohol 0.50 Silicone emulsion 0.40 Cyclomethicone 1.00 Dimethyl distearyl ammonium chloride 0.10 Purified water Balance Sum 100.00

[Formulation Example 3] Ointment in external preparation for skin

Ointments were prepared in a conventional manner according to the compositions shown in Table 11 below.

ingredient Content (% by weight) The extract of Example 1 2.00 glycerin 8.00 Butylene glycol 4.00 Liquid paraffin 15.00 Beta Glucan 7.00 Carbomer 0.10 Caprylic / capric triglyceride 3.00 Squalane 1.00 Cetearyl glucoside 1.50 Sorbitan stearate 0.40 Cetearyl alcohol 1.00 Wax 4.00 Purified water Balance Sum 100.00

[Formulation Example 4] Lotion

Lotions were prepared in a conventional manner according to the composition shown in Table 12 below.

ingredient Content (% by weight) The extract of Example 1 2.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Water soluble collagen (1% aqueous solution) 1.00 Sodium citrate 0.10 Citric acid 0.05 Licorice extract 0.20 1,3-butylene glycol 3.00 Purified water Balance Sum 100.00

[Formulation Example 5] Cream

Creams were prepared in a conventional manner according to the composition shown in Table 13 below.

ingredient Content (% by weight) The extract of Example 1 2.00 Polyethylene glycol monostearate 2.00 Self emulsifying monostearic acid glycerin 5.00 Cetyl alcohol 4.00 Squalene 6.00 Tri-2-ethylhexane glyceryl 6.00 Sphingoglycolipids 1.00 1,3-butylene glycol 7.00 Purified water Balance Sum 100.00

[Formulation Example 6] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 14 below.

ingredient Content (% by weight) The extract of Example 1 2.00 Polyvinyl alcohol 13.00 L-ascorbic acid-2-phosphate magnesium salt 1.00 Lauroylhydroxyproline 1.00 Water soluble collagen (1% aqueous solution) 2.00 1,3-butylene glycol 3.00 ethanol 5.00 Purified water Balance Sum 100.00

[Formulation Example 7] Cosmetic liquid preparation

The cosmetic formulations were prepared according to the conventional methods according to the compositions shown in Table 15 below.

ingredient Content (% by weight) The extract of Example 1 2.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 Concentrated glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 5.00 Purified water Balance Sum 100.00

[Formulation Example 8] Soft capsule

A soft capsule was prepared by mixing 50 mg of the extract of Example 1, 80 to 140 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of vegetable hydrogenated oil, 4 mg of yellow radish and 6 mg of lecithin per one capsule according to a conventional method .

[Formulation Example 9] Tablets

50 mg of the extract of Example 1, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluid bed drier, followed by addition of 6 mg of sugar ester and tabletting by tabletting.

[Formulation Example 10]

50 mg of the extract of Example 1, 250 mg of anhydrous crystalline glucose and 550 mg of starch were mixed and granulated into granules using a fluidized bed granulator, and filled in a vial to prepare granules.

[Formulation Example 11] Drinking agent

50 mg of the extract of Example 1, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide were mixed, and 300 ml of purified water was added to each bottle to fill 200 ml of each bottle. And then sterilized at 130 DEG C for 4 to 5 seconds to prepare a beverage drink.

Claims (13)

A ginseng extract comprising 2.5 to 4.5% by weight of ginsenoside Rb2, 3 to 7.5% by weight of ginsenoside Rc, and 2 to 5% by weight of ginsenoside Rg1, based on the total weight of ginseng extract. The ginseng extract according to claim 1, wherein the ginseng extract further comprises ginsenoside Re in an amount of 6 to 20% by weight based on the total weight of the ginseng extract. The ginseng extract of claim 1, wherein the ginseng extract is extracted from the roots of ginseng. The ginseng extract of claim 1, wherein the ginseng extract is extracted from the root of ginseng. A method for producing ginseng extract according to claim 1,
Extracting the ginseng with a mixture of water and an organic solvent having a ratio of an organic solvent of 10 to 90% (v / v) to the ginseng, repeating the steps of pressure and vacuum extraction sequentially, and the organic solvent is extracted with ethanol, methanol, Ether, ethyl acetate and chloroform, the pressure during the pressure extraction is 1 to 3 kgf / cm ² , the pressure during the extraction under reduced pressure is 600 ± 50 mmHg, the extraction temperature is 65 to 85 ° C. In step; And
Dissolving the ginseng extract obtained in the above step in water, extracting it with ethyl acetate, removing the organic solvent layer, and extracting the water layer again with butanol to prepare ginseng extract;
&Lt; / RTI &gt; A ginseng extract prepared by the method of claim 5. A composition for promoting hair growth and hair growth comprising the ginseng extract according to any one of claims 1 to 4 as an active ingredient. [Claim 7] The composition for promoting hair growth and hair growth according to claim 7, wherein the ginseng extract is contained in an amount of 2 to 20 wt% based on the total weight of the composition. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the ginsenoside contained in the ginseng extract shortens the period of transition from the resting stage to the growing stage during the hair growth cycle. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a pharmaceutical composition. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a cosmetic composition. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is an external preparation for skin. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a food composition.

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