WO2015005700A1 - Composition for promoting hair sprouting and hair growth - Google Patents

Abstract

A composition for promoting hair sprouting and hair growth according to the present invention contains a higher content of ginsenoside Rb2, Rc, and Rg1, and thus can provide better hair sprouting and hair growth effects compared to hair growth agents manufactured by existing techniques. Also, the composition is made very safe by using plant-based natural products and thus can be used as a skin preparation for external application for promoting hair sprouting and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions, and food compositions, among others.

Description

Hair growth and hair growth promoting composition

The content described in the present specification relates to a ginseng extract with increased content of ginsenosides and a composition for promoting hair growth and hair growth which promotes hair growth by shortening the period of transition from the resting phase to the growth phase.

Recently, hair loss population is gradually increasing due to the increase of social stress, westernized eating habits such as environmental pollution and instant food, frequent perm and dyeing. Hair cycles include anagen, which grows hair, catagen, which ends growth and hairball shrinks, talogen, which is the time when the nipple stops working and the hair stays in the scalp. It can be divided into a generator, which is a time to start or to dehair old hair by generating new hair.

The anagen stage (2-7 years) is the period of hair growth, which is divided into the stages of hair production, from which hair exits the hair follicles and the formation of hard keratin in the hair follicles. Hair continues to grow self-development. Cataract Stage (two to three weeks) is the period when the growth phase ends and metabolism slows down while maintaining the shape of the hair. This stage does not produce keratin. The degenerative stage accounts for 1% of all hair. At this time, the hair follicle contracts and is divided into the dermal papilla and surrounded by the hair follicle and rises upward, and the cell division is at a standstill. The Talogen Stage (3 months) is followed by atrophy of the nipples, the hair follicles clumping gradually, and the roots being pushed upwards. The period of hair loss is 3-4 months until the next growth stage begins.

Alopecia is a lot of hair in the resting phase, while the normal person has a lot of hair in the growth phase, and the hair loss is visible. As hair loss progresses, the growth phase is shorter, which leads to smaller and smaller hairs. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting state to go to the growth phase quickly and to increase the shortened growth phase.

Androgenetic alopecia is caused by a male hormone called Testosterone, which is converted into a more powerful hormone, Dihydrotestosterone (DHT), by an enzyme called alpha-reductase. Hormones act on the hair follicles, leading the hair follicles from the growth phase to the degenerative phase, leading to hair loss. Therefore, a method of inhibiting the production of DHT by 5 alpha-reductase is mainly used to treat androgenetic alopecia.

Gynecologic alopecia is mainly caused by a decrease in the amount of estrogen after menopause. Minoxidil and estrogen are mainly used as treatments for gynecomastia.

Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition. This alopecia areata is fundamentally different from androgenetic alopecia, and the treatment is also different, using a method of treating corticosteroids, or applying minoxidil to the affected area or artificially causing irritation in the affected area.

As for the various and complex causes of hair loss, ingredients for promoting blood circulation, inhibiting the action of testosterone, and strengthening the hair root function, etc. are sold as products on the market, but there are still no clear effects and problems of side effects. May be raised. For example, minoxidil has been reported to cause sticky feeling and irritation to the skin. Finasteride is currently used as an oral preparation, but side effects such as sexual dysfunction have been reported according to its intake. The effect can be expected only by oral administration, there was a lot of inconvenience in use.

[Preceding technical literature]

[Patent Documents]

Korean Patent Publication No. 10-2011-0000433

In order to solve the above problems, the present invention is ginseng extract with increased content of ginsenosides, and promotes hair growth and hair growth with safety while promoting the growth of hair by shortening the period of transition from the resting phase to the growth phase comprising the same It aims to provide the composition for this.

In order to solve the above object,

An embodiment of the present invention provides a ginseng extract comprising at least 2.5% by weight of ginsenoside Rb2, at least 3% by weight of ginsenoside Rc and at least 2% by weight of ginsenoside Rg1 based on the total weight of the ginseng extract. .

In addition, an embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re in the ginseng extract.

Another embodiment of the present invention is a manufacturing method of the ginseng extract,

Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting sequentially performing repeated pressurization and reduced pressure extraction processes; and

The ginseng extract obtained by the above step is dissolved in water and extracted with an organic solvent to remove the organic solvent layer, and the water layer is extracted with an organic solvent again provides a method for producing a ginseng extract comprising the step of producing a ginseng extract. .

Another embodiment of the present invention provides a composition for promoting hair growth and hair growth comprising a ginseng extract with an increased content of ginsenosides as described above.

Hair growth and hair growth promoting composition according to the present invention by containing a large amount of ginsenosides Rb2, Rc and Rg1 or Rb2, Rc, Rg1 and Re in an increased concentration, it can exhibit a hair growth and hair growth promoting effect of the conventional hair regrowth agent or more have.

In addition, since the use of vegetable natural products is excellent in safety, it can be used as an external preparation for promoting hair growth and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions and food compositions.

Figure 1 is a structural formula showing the chemical structure of each type of ginsenoside according to an embodiment of the present invention.

Figure 2 is an HPLC analysis graph of ginsenosides contained in the ginseng extract according to an embodiment of the present invention.

Figure 3 is an HPLC analysis graph of ginsenosides contained in the general ginseng extract of the comparative example of the present invention.

Figure 4 is a graph showing the increased expression of the hair growth factor VEGF in the dermal papilla cells of ginseng extract (Example 1), Comparative ginseng extract (Comparative Example 1) according to an embodiment of the present invention.

5 is a graph showing the dermal papilla cell proliferation effect (hDPc proliferation) of the ginseng extract (Example 1), the comparative ginseng extract (Comparative Example 1) according to an embodiment of the present invention.

Figure 6 is a graph showing the hair follicle dermal papilla cell proliferation effect (hDPc proliferation) according to the ginsenoside type of the main component of the ginseng extract according to an embodiment of the present invention.

Figure 7 is a view showing the hair growth promoting effect of the ginseng extract (Example 1), the general ginseng extract of Comparative Example (Comparative Example 1) in the human hair follicle organ according to an embodiment of the present invention.

8 is a ginseng extract according to an embodiment of the present invention (Example 1), the general ginseng extract (Comparative Example 1) of the comparative example, ginsenoside Rb1, Rb2, Rc, Rd, Re alc Rg1 in human hair follicle organs Figure showing the hair growth promoting effect.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

An embodiment of the present invention provides a ginseng extract comprising at least 2.5% by weight of ginsenoside Rb2, at least 3% by weight of ginsenoside Rc, and at least 2% by weight of ginsenoside Rg1. .

An embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re with respect to the ginseng extract.

Ginsenoside is a type of saponin, a type of glycoside, and is derived from the roots, stems, leaves, bark, and seeds of plants. Ginsenosides include various kinds of ginsenosides, for example, Rb1, Rb2, Rc, Rd, Re, and Rg1. Ginsenosides are present according to plant types, growing conditions, processing conditions, extraction methods, and ginseng parts. The types and contents of cenosides are different. Figure 1 shows the chemical structure of each ginsenoside.

Ginsenoside can be divided into PPT (Re, Rg1), PPD (Rb1, Rb2, Rc, Rd) according to its structure, and Rb1, Rb2, Rc, Rd, Re and Rg1 are natural ginseng, that is, red ginseng It is a water-soluble, polar substance with no denaturation with sugars removed through oxidation.

Another embodiment of the present invention provides a ginseng extract extracted from the root of the ginseng portion as the ginseng extract as described above. Specifically, it provides a ginseng extract extracted from the ginseng cup root (misam) having a high yield of ginsenosides.

As an embodiment of the present invention, each of the ginsenosides ginsenoside Rb2 may be included in more than 2.5% by weight based on the total weight of the ginseng extract, ginsenoside Rc may be included in more than 3% by weight, ginsenosides Re may be included in more than 6% by weight, ginsenoside Rg1 may be included in more than 2% by weight. Ginsenoside Rb2 may be provided with a ginseng extract containing 2.5 to 4.5% by weight, Rc to 3 to 7.5% by weight, Re to 6 to 20% by weight and Rg1 to 2 to 5% by weight.

According to one embodiment of the present invention, ginsenoside Rb2, 2.5% by weight or more, 3% by weight of Rc, 6% by weight of Re and 2% by weight or more of Rg1 based on the total weight of the ginseng extract as described above. Ginseng extract can be prepared using any method so long as it can extract components such as ginsenosides from ginseng. For example, it can manufacture by the pressure circulation type extraction process which performs a pressurization process or a pressure reduction process, the solvent fractionation method, etc., and these can also be performed continuously. At this time, the pressure-circulating extraction process may be specifically carried out by adding water, an organic solvent or a mixture thereof to ginseng and circulating the pressurized and reduced pressure at a predetermined time.

More specifically, the present invention can be prepared by the following method ginseng extract having a high content of ginsenosides Rb2, Rc and Rg1 as an example.

Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting while sequentially performing pressure and pressure extraction processes; and

The ginseng extract obtained by the above step is dissolved in water, extracted with an organic solvent, the organic solvent layer is removed, and the ginseng extract may be prepared by a method comprising extracting the water layer with an organic solvent again.

Specifically, the type of solvent that can be used as one embodiment of the present invention is not particularly limited, and may be, for example, water, an organic solvent, or a mixed solvent of water and an organic solvent. For example, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform. For example, the mixed solvent of water and the organic solvent may have a ratio of 10 to 90% (v / v) of the organic solvent. More specifically, the solvent may be an extract extracted using 10 to 90% (v / v) ethanol, more specifically 70% (v / v) ethanol.

In addition, the pressurized and reduced pressure extraction process according to an embodiment of the present invention can be extracted for a total of 1.5 to 2.5 hours while cyclically performing the pressurized and reduced pressure extraction process for 20 to 40 minutes each with a pressure-circulating extraction device. . In addition, the pressure at the time of pressurized extraction according to an embodiment of the present invention is 1 to 3 kgf / cm ² , the pressure at the reduced pressure to be 550 to 650 mmHg, and the extraction temperature is 65 to 85 ° C.

In the case of carrying out the pressure reduction and pressure repeated circulation processes as described above, ginsenoside modification, for example, ginsenoside Rb1 to Rg3 may be minimized by minimizing thermal denaturation of ginseng. Therefore, the extraction yield of the ginseng extract can be improved by 25 to 30% compared to the case of extracting without repeating the existing simple extraction apparatus. In addition, by using an organic solvent such as ethyl acetate to remove the non-polar material, it is possible to maximize the content of ginsenosides by extracting the water layer with an organic solvent such as butanol. Specifically, according to the solvent fractionation method, the ginseng extract in general ginseng extract is 1.5 to 2% by weight of total ginsenosides than 10 times more than conventional, specifically 15 to 25 It can be improved by weight.

One embodiment of the present invention by providing a composition for promoting hair growth and hair growth comprising the ginseng extract as described above can effectively shorten the cycle from the resting phase to the growth phase of the hair growth cycle, comprising a general ginseng extract Compared to the case, the hair growth promoting effect is remarkably excellent. Therefore, it is possible to achieve the effect of promoting excellent hair growth and hair growth that did not exist before.

According to one embodiment, the present invention provides hair growth and hair growth promoting purpose of the ginseng extract described in the specification of the present invention as described above, or hair growth and hair growth promoting use of the composition comprising the ginseng extract.

In addition, another embodiment of the present invention provides a method for promoting hair growth and hair growth comprising administering to the subject an effective amount of the ginseng extract described in the specification of the present invention as described above, wherein the ginseng extract in the composition And may be administered.

Furthermore, another embodiment of the present invention can provide the ginseng extract described in the specification of the present invention as described above for use in promoting hair growth and hair growth, wherein the ginseng extract may be used in the composition.

According to one embodiment of the invention, the composition is not particularly limited to the amount of ginseng extract including ginsenosides Rb2, Rc and Rg1, for example, may comprise 2% by weight or more based on the total weight of the composition For example, it may include 2 to 20% by weight. If the ginseng extract is included in less than 2% by weight can not be expected sufficient hair growth effect, if it contains more than 20% by weight because of difficulty in the safety or formulation formulation.

The composition according to the embodiment of the present invention may be, for example, a pharmaceutical composition, cosmetic composition or health food composition.

The pharmaceutical composition for promoting hair growth and hair growth according to an embodiment of the present invention is a preservative, stabilizer, hydrating or emulsifying accelerator, pharmaceutical supplements such as salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances And may be formulated in various oral or parenteral dosage forms according to conventional methods.

The oral dosage forms include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, and the like, and these formulations include surfactants in addition to the active ingredients. , Diluents (eg lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), glidants (eg silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols). . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt Pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods. In addition, the parenteral dosage form may be a transdermal dosage form, for example, a preparation such as an injection, a drop, an ointment, a lotion, a gel, a cream, a spray, a suspension, an emulsion, a suppository, or a patch. May be, but is not limited thereto.

The pharmaceutical composition according to an embodiment of the present invention may be administered parenterally, rectally, topically, transdermally, subcutaneously, and the like. Pharmaceutical compositions according to one embodiment of the invention may be administered topically to the scalp, for example.

Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug depends on various factors such as less progression, onset, age, health condition, complications, etc. of the subject to be administered. Generally, the composition may be administered by dividing 1 μg / kg to 200 mg / kg, preferably 50 μg / kg to 50 mg / kg, once or three times a day, and the dosage may be determined by any method. Nor does it limit the scope of the invention.

The hair growth and hair growth promoting composition according to an embodiment of the present invention may be a cosmetic composition, the appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing It may be formulated into cosmetics such as creams, cleansing foams, cleansing water, packs, powders, body lotions, body creams, body oils and body essences.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

According to one embodiment of the present invention, the content of the active ingredient is not particularly limited, but may include an extract including the active ingredient in an amount of 0.001 to 20% by weight based on the total weight of the composition. If the content is satisfied it can exhibit excellent efficacy without side effects.

The cosmetic composition according to an embodiment of the present invention may further include a functional additive and a component included in the general cosmetic composition in addition to the extract containing the active ingredient. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

In addition to the said functional additive, you may mix | blend the component contained in a general cosmetic composition with the cosmetic composition of this invention as needed. In addition to the other components included, oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation And accelerators, cooling agents, limiting agents, purified water, and the like.

Furthermore, the composition for promoting hair growth and hair growth according to an embodiment of the present invention is an external preparation for skin, and the external preparation for skin may be included herein as a generic term that may include anything applied to the outside of the skin. have.

In addition, the hair growth and hair growth promoting composition according to an embodiment of the present invention may be a health food composition, ginsenoside extract comprising ginsenosides Rb2, Rc, Re, Rg1 as an active ingredient It preferably comprises 0.001 to 20% by weight relative to the total weight of the composition. When the extract is less than 0.001% by weight, the efficacy does not appear sufficiently, and when the extract exceeds 20% by weight, the efficiency of the efficacy due to the addition of the active ingredient is lowered.

In the health food composition according to an embodiment of the present invention, the composition may be a liquid or solid formulation, tablets, capsules, soft capsules, pills, granules, beverages (drinks), diet bar, chocolate, It may be a caramel formulation or a confectionary formulation, and the formulation is not particularly limited. The health food composition of the present invention, the health food composition may contain an excipient, sugars, flavorings, pigments, fats and oils, proteins and the like as necessary in addition to the active ingredient.

Hereinafter, the present invention will be described through the following examples and test examples. Examples and test examples are intended to explain the present invention in more detail, but the scope of the present invention is not limited to the scope of the following examples.

In addition, it is obvious that any person skilled in the art can make various modifications and imitations within the scope of the appended claims without departing from the scope of the technical idea of the present invention.

Example 1 Preparation of Ginseng Extract Including Ginsenoside Rb2, Rc, Rg1 and Re

Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 20 g of 50% ethanol was added to 2 g of the obtained ginseng powder, and the extract was pressurized and depressurized by a pressure-circulating extractor for 30 minutes, and extracted for 2 hours in total. At this time, the pressure during extraction was 2kgf / cm ² , the pressure during extraction under reduced pressure was 600 ± 50 mmHg, and the extraction temperature was set at 75 ° C. The ginseng extract obtained by the said process was filtered, the supernatant was dried under reduced pressure and 0.57 g of dry weight was obtained. Then, the dried product was dissolved in water, extracted with ethyl acetate to remove the ethyl acetate layer, the water layer was extracted with butanol and dried under reduced pressure to obtain a dry weight of 2.7g. This was set as Example 1.

In addition, the ginsenosides contained in the ginseng extract of Example 1 were analyzed by HPLC with the following analyzer and conditions, and the results are shown graphically. And from this result, the content of each component of ginsenoside contained in the extract is shown in Table 2 and Figure 2 by weight.

HPLC analysis equipment and analysis conditions

Instrument: Waters 2998 PDA detecter, 1525 pump, 2707 autosampler

Column: Sun fire C18 305um, 4.6 X150 mm

Detector: UV 203nm

Flow rate: 1 mL / min

Injection volumn: 20ul

Condition: gradient (A-water, B-acetonitrile)

Table 1

Time (min)	A (water)	B (acetonitrile)
0	82	18
22	82	18
32	70	30
60	50	50

TABLE 2

division	content(%)
	Rg1	Re	Rb1	Rc	Rb2	Rd
Ginseng Extract (Example 1)	2.1	6.23	3.52	3.14	2.91	1.58

As a result, as shown in Table 2 and FIG. 2, Example 1 of the present invention is 2.91% by weight of ginsenoside Rb2 of 2.5% by weight or more, 3.14% by weight of Rc of 3% by weight or more, 6.23% by weight of 6% or more of Re. % And Rg1 was confirmed to include 2.1% by weight or more 2.0% by weight.

Comparative Example 1 Preparation of General Ginseng Extract

Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 2 g of the ginseng powder obtained was extracted with 100 mL of 50% ethanol at 75 ° C. for 2 hours, and then the sample was filtered, and the supernatant was dried under reduced pressure to obtain a dry weight of 0.30 g. This was set as Comparative Example 1.

In addition, the ginsenosides contained in the ginseng extract of Comparative Example 1 were analyzed by HPLC with the same analyzer and conditions as in Example 1, and the results are shown graphically. And from this result, the content of each component of ginsenoside contained in the extract is shown in Table 3 and Figure 3 by weight.

TABLE 3

division	content(%)
	Rg1	Re	Rb1	Rc	Rb2	Rd
Ginseng Extract (Comparative Example 1)	0.14	0.16	0.67	0.55	0.33	0.13

As a result, as shown in Table 3 and Figure 3 Comparative Example 1 contains 0.33% by weight ginsenoside Rb2, 0.55 Rc, 0.16% by weight Re and 0.14% by weight Rg1, Example of the present invention It was confirmed that 1 contains about 5 to 15 times more content of each ginsenoside than the general ginseng extract. In addition, it can be seen that the total weight of ginsenosides contained in the ginseng extract also has a significantly higher value than Example 1 of Comparative Example 1.

Test Example 1 Expression of Hair Growth Factor VEGF in Hair Papillary Cells

To evaluate the extent to which ginseng extract expresses Vascular endothelial growth factor (VEGF), which is a growth factor necessary for angiogenesis, to move to the hair growth phase, an in vitro system is applied. The activity was evaluated after the application of ginseng extract.

For this experiment, 47-year-old male dermal papilla cells (DPC) (P.11) 4 X 10 ⁵ cells were seeded in a 12 well plate, and 10% FBS (fetal bovine serum). Cultured overnight in DMEM (Dulbecco's modified Eagle's medium) containing. After incubation, Example 1 and Comparative Example 1 were each treated with 20 ppm. DMSO was used as a negative control. After 24 hours, the mixed solution treated with Example 1 or Comparative Example 1 was collected (soup collect) and ginseng of Example 1 and Comparative Example 1 using VEGF ELISA (Vascular endothelial growth factor Enzyme-Linked Immunosorbent Assay; R & D biosystems) The difference in the expression level according to the extract was confirmed. And the result is shown in FIG.

As can be seen in Figure 4, Comparative Example 1, a general ginseng extract did not show a significant difference in effect compared to the control group did not use ginseng extract. However, Example 1 of the present invention increased the expression of the hair growth factor VEGF by at least about three times when used in the same concentration as Comparative Example 1. This is because the ginseng extract of Example 1 contained more ginsenosides Rb2, Rc and Rg1 than the general ginseng extract of Comparative Example 1, thereby effectively increasing the expression of hair growth factor VEGF in dermal papilla cells. Therefore, it can be seen that the ginseng extract according to the present invention has excellent hair growth promoting ability.

[Test Example 2] Evaluation of hair follicle dermal papilla cell proliferation effect

The keratin proteins that make up hair are produced in hair root keratinocytes, which are differentiated from dermal papilla cells. Therefore, if the ginseng extract according to the present invention promotes the activity of the dermal papilla cells, it may promote the activity of keratinocytes differentiated therefrom and further promote hair production.

In this experiment, the effect of promoting ginseng papilla cell activity of ginseng extract was evaluated as follows.

First, the human dermal papilla cell line was used in the present invention. The cell line was incubated for 24 hours in an incubator maintained at 5% CO ₂ , 37 ° C with Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gaithersburg, MD, USA) containing 10% bovine serum serum (FBS). The ginseng extract of Example 1 was treated with 10 ppm and 20 ppm, and the ginseng extract of Comparative Example 1 with 20 ppm, respectively. DMSO was used as a negative control. 24 hours after the test material was treated, the cell proliferation capacity (%) was measured using the WST-1 kit (Roche). The results are shown in FIG.

As can be seen in Figure 5, Example 1, the ginseng extract according to the present invention significantly increased the proliferation of both cells 10ppm and 20ppm. In particular, the case where 10 ppm of Example 1 was treated was about 1.5 times higher than that of 20 ppm of Comparative Example 1, which is a general ginseng extract. This means that the ginseng extract according to the present invention can promote the growth of the dermal papilla cells than the conventional ginseng extract, it can also promote the activity and hair production of keratinocytes more effectively.

Test Example 3 Evaluation of Hair Growth Promoting Effect Using Human Hair Follicle Organs

Experiments were conducted to confirm the hair growth promoting ability in the actual human hair follicle organ of the present invention.

First, ginsenosides Rb1, Rb2, Rc, Rd, Re and Rg1, which are the main constituents of ginseng extracts, were treated by 0.1μM and 1μM for each papillary cell in the same manner as in Test Example 2, and after 24 hours. Cell proliferation (%) was measured using the WST-1 kit (Roche). The results are shown in FIG.

Then, the follicular organs were separated one by one from the occipital scalp tissue of a 55 year old male, and then William E medium (L-glutamine (2 mM), insulin (10 µg / ml), hydrocortisone (40 ng / ml), antibiotic (1%), Incubated in antifungal agent (1%). The hair follicles grown on the third day after the culture were screened and cut into 3 mm, and the selected hair follicle tissues were treated with no drug as a control, and the experimental group was treated with 2 ppm and 5 ppm of ginseng extract obtained in Example 1, respectively. And Comparative Example 1 were divided into the case of 5ppm treatment. After 8 days of treatment, length measurement and photographing were performed. In addition, ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Comparative Example 1, and Example 1 were each treated with 5 ppm of hair follicle tissues in the same manner as described above. The results are shown in FIGS. 7 and 8.

As a result, as shown in Figure 6 ginsenosides of ginsenosides Rb1, Rc, Rg1 showed a distinct hair growth promoting effect. All of these are statistically significant.

In addition, as can be seen in Figure 7, the hair growth in the hair follicle organs treated with 5ppm ginseng extract showed a hair growth promoting effect better than Comparative Example 1 and the control group treated with the same concentration. In particular, the case of 1 showed that the average growth length was reduced compared to the control. In FIG. 8, the average growth length of ginsenosides Rb1, Rb2, Rc, Rd, Re, and Rg1 increased more than that of Comparative Example 1, but Example 1 showed the largest increase in average growth length.

Therefore, gross ginsenoside Rb1, Rc, Rg1 having a hair growth promoting effect than the conventional ginseng extract according to the results of FIG. Since it is included, it can be seen that the effect of promoting the length of hair growth in hair follicles is excellent.

[Test Example 4] Clinical Trial of Hair Loss Patients

After dividing the scalp essence including Example 1 and Example 1 components and the control group except the components of Example 1 among the 20 to 60 year old adult females with hair loss symptoms in the test group (Example 1) and 23 control groups , 1 evening 1 time was applied to the hair loss site to use. The application period was 16 weeks, and the hair growth effect was determined by subject's subjective evaluation, researcher's evaluation by clinical picture, and objective hair measurement method using non-invasive phototrichogram, hair density, hair thickness, hair growth rate, and Hair loss prevention and hair growth effects were evaluated by measuring the biological parameters of hair, including the number of hairs dropped. The study consisted of randomized, double-blind, active controlled clinical trials. The experimental results are shown in Tables 4 to 8 below.

Hair density of Example 1 and the control group was compared in Table 4. In the case of Example 1, the increase was 5.485% and 7.409% after 8 weeks and 16 weeks of use, respectively. The percentage of improved subjects was 86.364% and 86.364% after 8 and 16 weeks of use, respectively. In contrast, the control group increased by 1.615% and 1.334% after 8 and 16 weeks of use.

Hair growth rates of Example 1 and the control group were compared in Table 4. In the case of Example 1, 3.218% and 8.783% increased after 8 weeks and 16 weeks of use, respectively, and significant changes were observed after 16 weeks. The percentage of improved subjects was 68.182% and 81.818%, respectively. The control group increased by 2.480% and decreased by 1.142% after 8 and 16 weeks of use.

Table 4

	MEAN ± STDEV			% Improvement		% Improved subjects		Significance difference before use (p value)		Significant difference (p value)
Example	Before use	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later
Density (n / cm2)	111.045 ± 20.285	117.136 ± 19.833	119.273 ± 19.452	5.485	7.409	86.364	86.364	0.000 *	0.000 *	0.008 ＃	0.001 ＃
Growth rate (㎛ / day)	0.292 ± 0.056	0.301 ± 0.080	0.318 ± 0.044	3.218	8.783	68.182	81.818	0.098	0.004 *	0.496	0.025 **
Control	Before use	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later
Density (n / cm2)	123.826 ± 18.968	125.826 ± 23.796	125.478 ± 25.293	1.615	1.334	47.826	43.478	0.267	0.444	NA	NA
Growth rate (㎛ / day)	0.289 ± 0.047	0.297 ± 0.042	0.286 ± 0.046	2.480	-1.142	50.000	45.455	0.471	0.732	NA	NA

*: Paired samples t-test, p <0.05

**: Independent samples t-test, p <0.05

#: Mann-Whitney U test, p <0.05

The hair thickness of Example 1 and the control group was compared in Table 5. In the case of Example 1, 1.526%, 2.611%, 4.782%, 7.178% increased after 2 weeks, 4 weeks, 8 weeks, and 16 weeks of use, respectively, and significant changes were observed at all time points. The improved subject ratios were 72.727%, 81.818%, 95.455% and 95.455% after 2, 4, 8 and 16 weeks of use, respectively. The control group decreased by 0.903%, 0.765%, 0.595% and 1.084% after 2, 4, 8 and 16 weeks of use, respectively.

Table 5

	MEAN ± STDEV		% Improvement		% Improved subjects		Significance difference before use (p value)		Significance compared to the control group (p value)
Thickness (㎛)	Example	Control	Example	Control	Example	Control	Example	Control
Before use	0.080 ± 0.010	0.082 ± 0.011	NA	NA	NA	NA	NA	NA	NA
2 weeks later	0.082 ± 0.011	0.081 ± 0.011	1.526	-0.903	72.727	34.783	0.021 †	0.084	0.002 ＃
4 weeks later	0.083 ± 0.011	0.081 ± 0.010	2.611	-0.765	81.818	52.174	0.001 †	0.235	0.001 ＃
8 weeks later	0.084 ± 0.012	0.081 ± 0.011	4.782	-0.595	95.455	43.478	0.000 †	0.134	0.000 ＃
16 weeks later	0.086 ± 0.012	0.081 ± 0.010	7.178	-1.084	95.455	52.174	0.000 *	0.218	0.000 ＃

*: Paired samples t-test, p <0.05

†: Wilcoxon signed rank test, p <0.05

#: Mann-Whitney U test, p <0.05

The hair loss of Example 1 and the control group was compared in Table 6. In the case of Example 1, 1.589% increase after 4 weeks of use, 4.849% decrease after 8 weeks, and 33.278% decrease after 16 weeks, and significant changes were observed after 16 weeks. The improved subject rates were 50.000%, 54.545% and 72.727% after 4, 8 and 16 weeks of use, respectively. The control group increased 26.721% after 4 weeks of use, decreased by 1.953% after 8 weeks, and increased by 2.364% after 16 weeks.

Table 6

	MEAN ± STDEV		% Improvement		% Improved subjects		Significance difference before use (p value)		Significance compared to the control group (p value)
Falling Hair Repellent (ea)	Example	Control	Example	Control	Example	Control	Example	Control
Before use	54.364 ± 29.589	42.304 ± 20.328	NA	NA	NA	NA	NA	NA	NA
4 weeks later	55.227 ± 28.846	53.609 ± 38.577	1.589	26.721	50.000	43.478	0.903	0.102	0.452
8 weeks later	51.727 ± 36.482	41.478 ± 25.472	-4.849	-1.953	54.545	43.478	0.733	0.870	0.776
16 weeks later	36.273 ± 23.511	43.304 ± 34.189	-33.278	2.364	72.727	52.174	0.006 *	0.833	0.047 **

*: Paired samples t-test, p <0.05

**: Independent samples t-test, p <0.05

Dropout improvement of Example 1 and the control was compared to visual evaluation in Table 7. In Example 1, 0.045, 0.318, and 0.364 points increased after 4, 8, and 16 weeks of use, respectively, and significant changes were observed after 8, 16 weeks of use. The improved subject rates were 9.091%, 22.727%, and 36.364% after 4, 8, and 16 weeks of use, respectively. The control group decreased by 0.130 points, increased by 0.087 points, and decreased by 0.087 points after 4, 8, and 16 weeks of use. The improved subject rates were 0.000%, 17.391%, and 17.39% after 4, 8, and 16 weeks of use, respectively.

TABLE 7

	MEAN ± STDEV		% Improved subjects		Significance difference before use (p value)		Significance compared to the control group (p value)
Hair Loss Improvement (Score)	Example	Control	Example	Control	Example	Control
Before use	0.000 ± 0.000	0.000 ± 0.000	NA	NA	NA	NA	NA
4 weeks later	0.045 ± 0.375	-0.130 ± 0.344	9.091	0.000	0.564	0.083	0.110
8 weeks later	0.318 ± 0.646	0.087 ± 0.515	22.727	17.391	0.038 †	0.414	0.297
16 weeks later	0.364 ± 0.790	-0.087 ± 0.848	36.364	17.391	0.046 †	0.627	0.070

†: Wilcoxon signed rank test, p <0.05

* Hair loss improvement Visual evaluation: Very good (3), Better (2), Little better (1), No change (0), Little worse (-1), Bad (-2), Very bad (-3)

※ Since the value before use was 0, improvement rate could not be obtained and replaced with improved subject ratio.

The subjective improvement evaluations of Example 1 and the control group were compared in Table 8. In the case of Example 1, 40.9% and 63.6% were positively recognized after 8 weeks and 16 weeks of use, respectively, and negative recognition was only 0.0% and 4.4% for 8 weeks and 16 weeks of use, respectively. In the control group, 52.1% and 39.1% were positive after 8 weeks and 16 weeks of use, respectively, and 0.0% and 8.7% of 8 weeks and 16 weeks, respectively.

Table 8

	MEAN ± STDEV		Positive response rate (%)		% Of negative answers		P value compared to the control group
Example	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later
Hair Loss Improvement (Score)	2.455 ± 0.596	2.727 ± 0.767	40.909	63.636	0.000	4.545	0.486	0.087
Control	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later	8 weeks later	16 weeks later
Hair Loss Improvement (Score)	2.565 ± 0.590	2.348 ± 0.714	52.174	39.130	0.000	8.696	NA	NA

※ Subjective improvement degree: Very good (4), Good (3), Normal (2), Bad (1), Very bad (0)

* Positive answer rate (%): The proportion of subjects who answered very good (4) or good (3) among all the subjects

※ Negative answer rate (%): The proportion of the subjects who answered bad (1) or very bad (0) among all the subjects

In conclusion, Example 1 of the present invention has excellent hair loss prevention and hair growth effect compared to the control group only once a day as a result of the effectiveness evaluation.

Examples of the formulation of the composition according to the present invention are described below, but are also applicable to various other formulations, which are intended to explain in detail only and not intended to limit the present invention.

[Formulation Example 1] Shampoo in the external preparation for skin

To prepare a shampoo in a conventional manner according to the composition shown in Table 9.

Table 9

ingredient	Content (% by weight)
Extract of Example 1	2.00
Sodium lauryl sulfate	10.00
Cocamidopropyl Betaine	3.00
Carboxyl Vinyl Polymer	0.30
Polyquaternium-10	0.20
Cetyltrimethylammonium chloride	0.10
Purified water	Remaining amount
Sum	100.00

[Formulation Example 2] Rinse in external preparations for skin

To prepare a rinse in a conventional manner according to the composition shown in Table 10.

Table 10

ingredient	Content (% by weight)
Extract of Example 1	2.00
Cetyl alcohol	2.00
Stearyl alcohol	2.50
Behenyl Alcohol	0.50
Silicone emulsion	0.40
Cyclomethicone	1.00
Dimethyl distearyl ammonium chloride	0.10
Purified water	Remaining amount
Sum	100.00

[Formulation Example 3] Ointment in the external preparation for skin

The ointment was prepared in a conventional manner according to the composition described in Table 11.

Table 11

ingredient	Content (% by weight)
Extract of Example 1	2.00
glycerin	8.00
Butylene glycol	4.00
Liquid paraffin	15.00
Beta Glucan	7.00
Carbomer	0.10
Caprylic / Capric Triglycerides	3.00
Squalane	1.00
Cetearyl Glucoside	1.50
Sorbitan stearate	0.40
Cetearyl Alcohol	1.00
Beeswax	4.00
Purified water	Remaining amount
Sum	100.00

[Formulation Example 4] Lotion

The lotion was prepared in a conventional manner according to the composition described in Table 12 below.

Table 12

ingredient	Content (% by weight)
Extract of Example 1	2.00
L-ascorbic acid-2-magnesium phosphate	1.00
Water Soluble Collagen (1% Aqueous Solution)	1.00
Sodium citrate	0.10
Citric acid	0.05
Licorice Extract	0.20
1,3-butylene glycol	3.00
Purified water	Remaining amount
Sum	100.00

Formulation Example 5 Cream

To prepare a cream in a conventional manner according to the composition described in Table 13.

Table 13

ingredient	Content (% by weight)
Extract of Example 1	2.00
Polyethylene Glycol Monostearate	2.00
Self-emulsifying glycerin monostearate	5.00
Cetyl alcohol	4.00
Squalene	6.00
Tri2-ethylhexaneglyceryl	6.00
Sphingolipid	1.00
1,3-butylene glycol	7.00
Purified water	Remaining amount
Sum	100.00

[Formulation Example 6] Pack

To prepare a pack in a conventional manner according to the composition described in Table 14.

Table 14

ingredient	Content (% by weight)
Extract of Example 1	2.00
Polyvinyl alcohol	13.00
L-ascorbic acid-2-magnesium phosphate	1.00
Lauroylhydroxyproline	1.00
Water Soluble Collagen (1% Aqueous Solution)	2.00
1,3-butylene glycol	3.00
ethanol	5.00
Purified water	Remaining amount
Sum	100.00

Formulation Example 7 Cosmetic Liquid Formulation

To prepare a cosmetic liquid formulation in a conventional manner according to the composition shown in Table 15.

Table 15

ingredient	Content (% by weight)
Extract of Example 1	2.00
Hydroxyethylene cellulose (2% aqueous solution)	12.00
Xanthan gum (2% aqueous solution)	2.00
1,3-butylene glycol	6.00
Dark glycerin	4.00
Sodium Hyaluronate (1% aqueous solution) 5.00	5.00
Purified water	Remaining amount
Sum	100.00

Formulation Example 8 Soft Capsule

50 mg of extract of Example 1, 80-140 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of vegetable hardened oil, 4 mg of succinate and 6 mg of lecithin were mixed and 400 mg per capsule was prepared according to a conventional method to prepare a soft capsule. .

 Formulation Example 9 Tablets

50 mg of the extract of Example 1, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed dryer, and then, 6 mg of sugar ester was added and tableted with a tableting machine to prepare a tablet.

Formulation Example 10 Granules

50 mg of the extract of Example 1, 250 mg of anhydrous glucose and 550 mg of starch were mixed, molded into granules using a fluidized bed granulator, and then filled into a cloth to prepare a granule.

Formulation Example 11 Drinks

50 mg of the extract of Example 1, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide were mixed, and 300 ml of purified water was added thereto, and 200 ml of each bottle was filled. After filling the bottle sterilized for 4-5 seconds at 130 ℃ to prepare a beverage drink.

Claims (13)

1. A ginseng extract comprising at least 2.5% by weight of ginsenoside Rb2, at least 3% by weight of ginsenoside Rc and at least 2% by weight of ginsenoside Rg1 based on the total weight of the ginseng extract.
2. The ginseng extract of claim 1, further comprising ginsenoside Re in an amount of 6% by weight or more based on the total weight of the ginseng extract.
3. The ginseng extract of claim 1, wherein the ginseng extract is extracted from the root of ginseng.
4. The ginseng extract of claim 1, wherein the ginseng extract is extracted from a small root of ginseng.
5. Claim 1 of the manufacturing method of the ginseng extract,

   Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting while repeatedly performing pressure and pressure extraction processes; And

   Preparing a ginseng extract by dissolving the ginseng extract obtained by the above step in water, extracting with an organic solvent, removing the organic solvent layer, and then extracting the water layer with an organic solvent again;

   Method of producing ginseng extract comprising a.
6. Ginseng extract prepared by the method of claim 5.
7. Hair growth and hair growth promoting composition comprising the ginseng extract of any one of claims 1 to 4 as an active ingredient.
8. The composition for promoting hair growth and hair growth according to claim 7, wherein the ginseng extract is contained in an amount of 2% by weight or more based on the total weight of the composition.
9. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the ginsenosides included in the ginseng extract shorten the period of transition from the resting phase to the growth phase of the hair growth cycle.
10. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a pharmaceutical composition.
11. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a cosmetic composition.
12. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is an external preparation for skin.
13. 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a food composition.

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