KR100805852B1 - Method of producing ginsenoside enforced ginseng and method of producing red ginseng employing the same - Google Patents

Abstract

A method for preparing the insam (ginseng) with reinforced ginsenoside, a method for preparing the hongsam (insam steamed red) with reinforced ginsenoside, and a dietary supplement containing the insam or the insam steamed red are provided to increase the content of ginsenoside in insam or hongsam. A method for preparing the insam with reinforced ginsenoside comprises the steps of precipitating insam in an organic acid solvent; and decompressing it for 5-30 min. Preferably the organic acid solvent is selected from the group consisting of acetic acid, citric acid, lactic acid, oxalic acid, malic acid and ascorbic acid and its concentration is 0.5-2 M. A method for preparing the hongsam (insam steamed red) with reinforced ginsenoside comprises the steps of decompressing the insam precipitated in an organic acid solvent for 5-30 min; and steaming it.

Description

Method of Producing Ginseng Enhanced Ginseng and Method of Producing Red Ginseng Using The Same {Method of Producing Ginsenoside Enforced Ginseng and Method of Producing Red Ginseng employing the same}

1 is a photograph confirming the degree of organic acid solvent penetrating into the ginseng by natural acid permeation method (0 hours, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 14 hours, 18 hours) by time zone to be.

2 is a graph confirming the time-phase pH of ginseng immersed in an organic acid.

Figure 3 is a photograph confirming the degree of acid penetration by time when the ginseng immersed in an organic acid solvent for 5 to 30 minutes under reduced pressure.

Figure 4 is a graph confirming the pH change inside and outside the ginseng after decompression treatment of the ginseng immersed in the organic acid solvent.

Figure 5a is a graph showing the pH change of each solvent before and after the decompression treatment, Figure 5b is a graph showing the degree of pH change of the solvent due to the pressure reduction treatment.

6 is a photograph showing a process of preparing red ginseng using decompression ginseng in an organic acid solvent.

Figure 7 shows the TLC pattern of each red ginseng according to the organic acid reduced pressure treatment. In each pattern, 1 = citric acid, 2 = acetic acid, 3 = lactic acid, 4 = oxalic acid, 5 = malic acid, 6 = ascorbic acid acid), 7 = control.

The present invention relates to a ginsenoside-enhanced ginseng production method and a method for producing red ginseng using the same, more specifically, ginsenoside-enhanced ginseng comprising the step of depressurizing ginseng precipitated in an organic acid solvent. It relates to a manufacturing method and a method for producing red ginseng which is treated with red ginseng.

Ginseng has been used for many years as the most precious herbal ingredient in the Far East. The chemical composition and pharmacological effects of ginseng have been found to be the main active ingredient by modern scientific studies. The saponin component has 1-4 sugars bound to the Dammarane skeleton, which is different from the saponins of other plants. In particular, about 30 kinds of saponin components are known in Korean ginseng, and high content among them is ginsenosides Rb1, Rb2, Rc, Rd, Rg1, Re, and the like. These saponin components show a variety of drugs, the type and strength of the drug is very different depending on the structure.

Saponins are roughly classified into triteroid saponins and steroidal saponins according to the structure of their aglycone. Triteroid saponins are, again, oleic acid (oleanane) saponins and ginseng, which are mainly contained in streets and grasslands. It is classified as a dammarane saponin. Dammarane-based saponins, the major indicators of ginseng, are divided into three groups according to their chemical structure: Protopanaxadiol (PPD), Protopanaxatriol (PPT), and Oleanolic acid.

About 40 species of ginsenosides have been found so far, and have a wide range of effects on the central nervous system, the endocrine system, the immune system, and the metabolic system. These ginsenosides have similar or opposite actions, and it is known that certain components exert various effects (health functionalities) alone or through various kinds of interactions.

For example, it is estimated that gnsenoside-Rb1, a representative of PPD-based saponins, has a inhibitory effect on the central nervous system, and ginsenoside-Rg1, a representative of PPT-based saponins, has a promoting effect. Each of these components does not antagonize each other but has a characteristic of selective action. In particular, as a major microsaponin component of Korean red ginseng, it has strong proliferation inhibitory activity against various cancer cells (Kitagawa et al. 1993, Kikuchi et al. 1991, Ota et al. 1991) and promotes the differentiation of cancer cells into normal cells. G-Rh2 expressing activity (Odashima et al. 1979, Lee et al. 1993) and inhibits metastasis of cancer cells to normal cells (Kim et al., Archives of Pharmacal Research, p.253-269) Inhibitory activity (Park et al. Rhee, Arches of Pharmacal Research, 19, pp. 213-218, 1996) and platelet aggregation inhibitory activity (Isora Lee, Jung Il Park, Korean Ginseng Society, pp.132-140, 1997) and hypertension et al., Korean Ginseng Society, pp.416-423, 2002), memory enhancement (Sao Hai Ying et al., Archives of Pharmacal Research 28, pp.335-342, 2005), antithrombotic action, inhibition of liver injury, anticancer agents In recent years, G-Rg3, which has resistance suppression and vascular relaxation, has attracted much attention in academia.

As mentioned above, ginseng contains 34 kinds of ginsenosides (22 kinds of white ginseng and 30 kinds of red ginseng), but the content of each ingredient is too low to affect the efficacy by having different effects. If ginseng products are highlighted, the excellence of ginseng can be easily appealed to consumers.

In particular, it has been known that Rg3 inhibits cancer cell metastasis, inhibits platelet aggregation and antithrombotic action, experimental liver injury inhibitory action, vascular relaxation action, and anticancer drug resistance.

Ginsenoside Rg3 inhibits the production of interleukin-1beta, tumor necrosis factor-alpha, and nitric oxide in rat microglia. PROCEEDINGS OF THE CONVENTION OF THE PHARMACEUTICAL SOCIETY OF KOREA (Won TaeJoon, Hwang KwangWoo, Lee DoIk, Joo SeongSoo,). -2587, Vol. 30, No. 2, pp.221-225, 2004). The effects of fermented ginseng extract enhanced with ginsenoside Rg3 on blood pressure in patients with high or normal hypertension were investigated. Ginseng Society: A study on the effect of ginsenoside Rg3 on platelet aggregation and its mechanism of action (Stavro P. Mark, Vuksan Vladimir, pp.416-423, 2002) (Korea Ginseng Journal, 1226-8453, Vol. 21, no.2, pp.132-140, 1997, Lee So Ra, Park Jung Il ), The effects of ginsenosides Rg3 and Rh2 on the proliferation of prostate cancer cells are reported in Archives of Pharmacal Research (Kim Hyun-Sook, Lee Eun-Hee, Ko Sung-Ryong, Choi Kang-Ju, Park Jong-Hee, Im). Dong-Soon, 0253-6269, Vol. 27, No. 4, pp. 429-435, 2004). The increased memory and neuroprotective effects of selected ginsenosides were observed in Archives of Pharmacal Research (Sao Hai Ying, Zhang Jing, Yeo Soo). Jeong, Myung Chang Seon, Kim Hyang Mi, Kim Jong Moon, Park Jeong Hill, Cho Jung Sook, Kang Jong Seong, 0253-6269, Vol. 28, No. 3, pp.335-342, 2005). The effect of ginseng saponin on the regulation was found in Archives of Pharmacal Research (Park, Jong Dae, Kim, Dong-Sun, Hyeok-Young Kwon, Sang-Kwon Son, You-Hui Lee, Nam-In Baek, Shin-Il Kim, Dong). -Kwon Rhee, 0253-6269, Vol. 19, No. 3, pp. 213-218, 1996).

As described above, the effect of saponin of ginseng, in particular, the effect of Rg3 is surprising, therefore, the development of ginseng with enhanced function of ginsenosides has been required.

To this end, efforts have recently been made to process ginseng to change the specific efficacy and usefulness of ginseng. In particular, the structure of the saponin in such a process may change the drug efficacy.

For example, Korean Patent Publication No. 2000-058997 (October 6, 2000) discloses a method for producing acid-glycosylated ginseng that is easily absorbed using sulfuric acid or hydrochloric acid.

Korean Patent Laid-Open Publication No. 2003-0087250 (2003.11.14) discloses a method for processing ginseng containing ginsenosides specific to red ginseng, which increases the active ingredient of ginseng using ultra high pressure.

In addition, Korean Patent Publication No. 1996-017670 (May 23, 1996) shows that ginseng is processed at high temperature to produce processed ginseng with enhanced efficacy, unlike ginseng, which contains large amounts of ginsenosides Rg3 and Rg5. A method of making a composition is disclosed.

The above-mentioned prior art relates to a method of preparing ginseng containing a large amount of low molecular weight ginseng composition or a specific ginsenoside by acidification, ultra high pressure treatment or heat treatment of ginseng. However, the above methods have a disadvantage in that the enhancement efficiency of a specific saponin component is lowered or the energy efficiency of the manufacturing process is lowered because excessive high energy must be used to strengthen a specific component.

In addition, the inventions disclosed in the prior art are not all enhanced functionality to ginseng itself, but to impart functionality to the ginseng extract, or to impart functionality in the ginseng extraction process, there is a limit to the use of ginseng itself, such as red ginseng slices, red ginseng snacks There was.

Accordingly, the present inventors have intensively tried to develop a processing method of ginseng that can enhance specific ginsenosides more efficiently, and thus, the present invention has been completed.

It is an object of the present invention to provide a method for producing ginsenoside enhanced ginsenosides which is effective and economically.

Another object of the present invention is to provide a method for producing functional red ginseng, which is effective and economically enhanced with ginsenosides, which is useful for certain diseases.

Another object of the present invention to provide a health supplement comprising a functional red ginseng useful for cancer prevention and treatment.

In the present invention to achieve the above object

It provides a ginsenoside-enhanced ginseng manufacturing method comprising the step of reducing the ginseng precipitated in the organic acid solvent for about 5 ~ 30 minutes.

Ginsenoside reinforcing effect appears only after 5 minutes of decompression treatment, and ginsenoside reinforcing effect does not increase significantly over 30 minutes.

The reduced pressure condition according to the invention is preferably a reduced pressure condition with a vacuum degree of 25-100 mmHg at a temperature of 15-50 ° C.

The present invention also comprises the steps of depressurizing the ginseng precipitated in the organic acid solvent for 5 to 30 minutes; And it provides a method for producing ginsenoside-enhanced red ginseng comprising the step of increasing the ginseng under reduced pressure.

In the manufacturing method of the red ginseng, the red ginseng treatment is a) a step of steaming the pressure-reduced ginseng for 2 to 3 hours at a temperature of 94 ℃ ~ 100 ℃, preferably 96 ℃; b) cooling the steamed ginseng to room temperature; c) drying the cooled ginseng for 1 day to 2 days in a hot air dryer at 50 to 80 ° C .; And d) drying at a temperature of 40 ° C. to 60 ° C., preferably at 50 ° C. for 2 to 5 days.

This is to ensure that the ginseng is heated to the set temperature by the convection heat inside the steamer to increase the steaming. In case of increasing the temperature of red ginseng, the length of red ginseng may be longer. In case of increasing the temperature of red ginseng, the value of commodity may be impaired due to cracks on the skin of red ginseng.

The present invention also includes ginsenoside-enhanced red ginseng and a food acceptable food additive which are prepared by subjecting ginseng precipitated in an organic acid solvent to reduced pressure for 5 to 30 minutes, and by increasing the pressure-reduced ginseng. Provide health supplements.

In the present invention, the organic acid solvent may be any organic acid, preferably acetic acid, citric acid, lactic acid, oxalic acid, malic acid and ascorbic acid. At least one selected from the group consisting of) may be used.

In the present invention, the concentration of the organic acid solvent is preferably 0.1 ~ 2M.

The titration range of the organic acid was based on the appropriate concentration that can be added to the food, with the specific ginsenoside content (Rg3) increasing first.

Through the following examples will be described the present invention in more detail. These examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

1. Ginseng Sample Preparation

The ginseng sample for the practice of the present invention was used to buy a large amount of 4-5 ginseng ginseng in Anseong ginseng cooperatives and stored at 4 ℃.

2. Confirmation of organic acid penetration into ginseng

Comparative example  1: Natural Acid Penetration

Add 0.01-0.05% safranine staining solution to visually check the penetration of organic acid solvent into 1M ascorbic acid solution, and then immerse ginseng and check the time-phase pH and safranin penetration into ginseng. It was.

Acid penetration into the ginseng extracts the ginseng immersed in the organic acid solvent at different times, cuts 2cm below the top of the ginseng's fuselage, obtains a cross section, and checks the penetration of the safranin dye solution on the cross section. Solvent penetration was confirmed (FIG. 1).

In order to measure the pH, ginseng immersed in the organic acid solvent was taken out at different times, and homogenized by adding 9 mL of distilled water per 1 g of sample to prepare a ginseng solution, and then a glass electrode pH meter (Coring 430, USA) was added to the ginseng solution The measurement was carried out (Fig. 2).

Check the result:

1 is a photograph confirming the degree of organic acid solvent penetrating into the ginseng by natural acid permeation method (0 hours, 1 hour, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, 14 hours, 18 hours) by time zone to be.

Through Figure 1, it was confirmed that the dyeing to the inside of the ginseng for about 2 hours can be seen almost 4 hours after the dyeing inside. The results of 10 hours show that the ginseng must pass through a thick layer to penetrate into the inner layer of ginseng, and it takes considerable time to penetrate the organic acid solvent through this layer. In addition, since no further acid penetration after 10 hours, it was concluded that the natural acid treatment method is not suitable to penetrate organic acids to the inside of ginseng.

2 is a graph confirming the time-phase pH of ginseng immersed in an organic acid. Looking at the pH shown in Figure 2 it was confirmed that the acid penetrated from 1 hour after ascorbic acid immersion, the pH is rapidly lowered during the initial 2 hours. However, it can be seen that the acid penetration is hardly achieved after 2 hours.

Example  1 ~ 5: Acid penetration check through reduced pressure treatment

In order to visually check the penetration of the organic acid solvent into the 1M ascorbic acid solution, 0.01-0.05% safranine staining solution was added, and then ginseng was immersed, placed in a large-volume flask, and then subjected to reduced pressure.

Decompression treatment was carried out for each of 5 minutes, 10 minutes, 15 minutes, 20 minutes, and 30 minutes (Examples 1 to 5). Decompression conditions were set at a vacuum degree of 25-100 mmHg at a temperature of 15-50 ° C.

Acid penetration into the ginseng extracts the decompressed ginseng by time, cuts 2cm below the top of the ginseng fuselage and obtains a cross section, confirming the penetration of the organic acid solvent into the inside of the ginseng by confirming the penetration of the safranin dye solution on the cross section. It was.

The pH of the ginseng treated under reduced pressure for each time period was measured by dividing the internal and external mixture of ginseng. pH was measured by homogenizing 9 mL of distilled water with 1 g of sample to prepare a ginseng solution, and then putting a glass electrode pH meter (Coring 430, USA) into the ginseng solution.

In addition, the external ginseng measurement was performed in the same way as the internal ginseng measurement to measure the inside of the ginseng.

Check the result:

Figure 3 is a photograph confirming the degree of acid penetration by time when the ginseng immersed in an organic acid solvent for 5 to 30 minutes under reduced pressure. As can be seen in FIG. 3, when the organic acid solvent was penetrated through the reduced pressure treatment, the acid penetrated quickly into the ginseng after 5 minutes. After about 15 minutes, acid penetrated into the inner layer of ginseng.

Figure 4 is a graph confirming the pH change inside and outside the ginseng after decompression treatment of the ginseng immersed in the organic acid solvent. From Figure 4, the pH outside the ginseng was greatly reduced after 5 minutes, it can be seen that after 5 minutes shows a constant pH. In addition, it can be seen that the acid can penetrate into the ginseng through the decompression treatment for about 30 minutes through the graph of the change in the internal pH of ginseng.

Example  6-11: Fresh ginseng after decompression treatment according to the type of organic acid pH  Confirm change

After measuring the pH of 1M solution for each organic acid using acetic acid, lactic acid, citric acid, ascorbic acid, malic acid and oxalic acid (Examples 6 to 11, respectively), 250 g of fresh ginseng was put in the organic acid solvent and subjected to reduced pressure for 30 minutes.

The pH of the organic acid solvent was measured by dividing the lower part 2 cm from the top of the body of ginseng by dividing the inside and outside of the ginseng. pH was measured by adding 9 mL of distilled water to 1 g of the sample to homogenize to prepare a ginseng solution, and then putting a glass electrode pH meter (Coring 430, USA) into the ginseng solution.

This experiment is to confirm the change of pH of organic acid after decompression treatment according to the type of organic acid. If the fresh ginseng leaves the organic acid and the organic acid penetrates into the fresh ginseng when the vacuum is decompressed, the pH of the organic acid is The pH value was determined to be increased when the product was decompressed and confirmed through this experiment.

Figure 5a is a graph showing the pH change of each solvent before and after the decompression treatment, Figure 5b is a graph showing the degree of pH change of the solvent due to the pressure reduction treatment. The pH after acid solvent infiltration due to the reduced pressure treatment showed that the water content of fresh ginseng was replaced with the acid solution, and the pH was increased by about 0.1.

Example  12 ~ 17: Preparation of Red Ginseng by Decompression Treatment in an Organic Acid Solvent

The freshly washed ginseng was immersed in each of 1 M citric acid, acetic acid, lactic acid, oxalic acid, malic acid, and organic acids of ascorbic acid (Examples 12 to 17), followed by depressurization for 30 minutes to prepare red ginseng. The red ginseng was adjusted to 96 ° C. to 98 ° C. in such a manner that the pressure-reduced fresh ginseng was not subjected to pressure. After lowering to 50 ℃ it was prepared by drying for about 72 hours.

Comparative example  2: Preparation of Common Red Ginseng

Red ginseng was prepared in the same manner as in Example 12 except that the organic acid treatment and the reduced pressure treatment were not performed.

Comparative example  3: Preparation of Red Ginseng After Organic Acid Treatment

Red ginseng was prepared in the same manner as in Example 12 except that the pressure reduction treatment was not performed.

Results: Appearance Comparison

6 is a photograph showing a process of preparing red ginseng and red ginseng prepared using decompressed red ginseng in an organic acid solvent. 7 is a photograph comparing the appearance of red ginseng prepared according to Examples 12 to 17 and Comparative Example 1 by dividing after steaming and after drying.

When comparing the appearance of the red ginseng prepared after penetrating the organic acid through the decompression treatment of the fresh ginseng through the photograph of FIG. 6, the red ginseng prepared without the acid treatment and the other red ginseng treated with the organic solvent did not have a big difference in appearance, but oxalic acid Red ginseng treated with and ascorbic acid showed a dark color close to black, and there was a difference in appearance.

Result: compare

In order to analyze the saponin content of red ginseng prepared according to an organic acid solvent, 100 ml of 80% methanol was added to 2 g of a powder sample made by freeze-drying red ginseng prepared in Examples 6 to 17 and Comparative Examples 1 and 2 into powder. Then, the mixture was repeatedly extracted twice at 80 ° C. for 3 hours using a reflux condenser. The extracts were collected and concentrated under reduced pressure in 20 ml of distilled water, which was then used for the saponin extract.

The powder sample extract was transferred to a 250 ml aliquot, and the concentrate was washed with 20 ml of ethyl ether, and extracted three times using 20 ml of saturated butanol. The extracted water-saturated butanol was collected together, transferred to an aliquot, and washed twice with 60 ml of distilled water. Then, the butanol layer was transferred to a concentrated water, concentrated under reduced pressure, and then dissolved in 2 ml of methanol, and filtered to 0.45 μm, and used as a sample for analyzing total saponin content and ginsenoside composition of saponin.

This component analysis is shown in Table 1.

% Investigation Total Saponin%  Comparative Example 1 3.433 ± 0.00 1.91 Comparative Example 2 Example 12 (citric acid) 3.354 ± 0.06 2.48 Example 13 (acetic acid) 3.404 ± 0.05 1.75 Example 14 (lactic acid) 2.923 ± 0.15 1.66 Example 15 (oxalic acid) 2.604 ± 0.31 1.33 Example 16 (Malic Acid) 1.995 ± 0.13 0.94 Example 17 (ascorbic acid) 3.983 ± 0.09 1.96

1) of red ginseng Investigation (Crude Saponin A) content analysis

After analyzing the red ginseng prepared under reduced pressure in the organic acid solvent, the content of the irradiated saponinin did not show a significant difference in comparison with the red ginseng control prepared without the organic acid decompression treatment. According to the type of organic acid used, the content of irradiated saponin was much different. Ascorbic acid had higher contents of irradiated than the comparative example without organic acid treatment. . In particular, in malic acid, the content of irradiated phonine was about 1.5% lower than that of the comparative example.

2) red ginseng Total Saponin (Total Saponin A) content analysis

0.3 ml of 8% vanillin solution was added to 100 μl of saponin extract of red ginseng for each treated organic acid, which analyzed the content of the irradiated phononine, and 4 ml of 75 ° C. sulfuric acid solution was added in cold water. After heating for 10 minutes at 60 ℃ color development and absorbance at 545 nm to determine the total saponin content from the standard curve. The standard curve was prepared by sequentially measuring ginsenoside Re (WAKO Chem., Japan) at a concentration of 0.2 to 1.0 mg / ml.

In the case of red ginseng prepared by adding acid to most ginseng, there was little difference in total saponin content. In the case of malic acid, irradiated and total saponin contents were relatively lower than those of other acids.

Experimental Example  1: specific Ginsenoside  Ingredient Enhancement Check

Samples of BuOH fractions of red ginseng of Examples 12 to 17 and Comparative Example 1 were weighed in the amount of 1 mg of ginseng powder, dissolved in 1 ml of MeOH, and filtered using a microfilter kit (0.45 μm). It was used as a sample solution in a vial. Ginsenoside standard to be confirmed among ginseng saponin components was used to make 1 mg / ml.

2-5 μl of each sample extract is deposited on a silica gel plate and developed underneath with chloroform: methanol: water (5: 4: 1, v / v). At the end of the development, spray 10% sulfuric acid solution and warm for 10 minutes at 110 ℃. The saponin component in the sample extract was checked by comparing the Rf value (movement distance) and color of the saponin standard.

Figure 7 shows the TLC pattern according to the organic acid reduced pressure treatment. When comparing the TLC pattern according to the acid treatment with the red ginseng that was not treated with acid through FIG. 7, the ginsenosides of Rg1, Re, and Rc were strengthened in the red ginseng treated under lactic acid, oxalic acid, and malic acid. Red ginseng, which was decompressed in citric acid and ascorbic acid, was fortified with Rg3. As such, the red ginseng according to the present invention subjected to the reduced pressure in the organic acid showed more types of TLC patterns than the red ginseng of Comparative Example 1 which was not subjected to the reduced pressure of the organic acid, and it was confirmed that the increase in the content of various ginsenosides was observed. .

Experimental Example  2: Ginsenoside  Strengthening degree measurement

The BuOH fraction samples of red ginseng prepared by Examples 6 to 12 and Comparative Example 1 were weighed in the amount of 1 mg of ginseng powder, dissolved in 1 ml of MeOH, and filtered using a microfilter kit (0.45 μm). It was used as a sample liquid in a vial.

The measurement method was carried out using HPLC to determine the concentration of 12 ginsenosides of Rg1, Re, Rf, Rb1, Rg2, Rh1, Rc, Rb2, Rb3, Rd, Rg2, and Rh2 to 0.1 mg / ml, respectively. Was measured. Table 2 below shows the analysis conditions of the used HPLC, and Table 3 and Table 4 show the results for each ginsenoside.

    Instrument JASCO HPLC system Colunm waters μ-Bodapak C ₁₈ Flow rate 1.6 ml / min Temperature the normal temperature Mobile phase Acetonitrile: H ₂ O = 3: 7 Detection UV detector (JASCO, Japan), abs 203 nm

Rg1 Re Rf Rb1 + Rg2 + Rh1 Rc Control 0.049 0.030 0.052 0.189 0.047 Citric acid 0.027 0.016 0.049 0.135 0.076 Acetic acid 0.020 0.017 0.044 0.151 0.043 Lactic acid 0.095 0.049 0.051 0.132 0.078 Oxalic acid 0.145 0.085 0.053 0.036 0.130 Malic acid 0.120 0.104 0.038 0.038 0.105 Ascorbic acid 0.013 0.013 0.042 0.148 0.043 Fresh ginseng 0.030 0.030 0.030 0.020 0.110

Rb2 Rb3 Rd Rg3 Rh2 Sum Control 0.052 0.017 0.040 0.028 0.002 0.506 Citric acid 0.098 0.029 0.049 0.135 0.001 0.614 Acetic acid 0.072 0.043 0.027 0.031 0.004 0.451 Lactic acid 0.082 0.011 0.029 0.017 0.002 0.546 Oxalic acid 0.092 0.015 0.032 0.018 0.002 0.609 Malic acid 0.057 0.009 0.021 0.020 0.016 0.526 Ascorbic acid 0.047 0.016 0.022 0.502 0.004 0.851 Fresh ginseng 0.090 0.020 0.050 0.040 - 0.38

Red ginseng according to the present invention showed a ginsenoside pattern significantly different from the red ginseng of Comparative Example 1, which was not treated with organic acids, and showed a difference according to each used organic acid.

In summary, the ginsenoside content changes showed that Rg1 was significantly increased by oxalic acid, malic acid and lactic acid, Rc and Re were enhanced by oxalic acid and malic acid, and Rg3 was citric acid and Increased by ascorbic acid. Therefore, it was confirmed that citric acid, lactic acid, oxalic acid, malic acid and ascorbic acid except for acetic acid among the organic acids used in the present invention have an effect of increasing the content of certain ginsenosides (Rg1, Rc, Re, etc.). Thus, by varying the acid treatment and reduced pressure conditions can be prepared red ginseng enriched with the desired specific ginsenoside components, according to the purpose can be produced red ginseng enhanced by the specific components as desired, which is unnecessary for a particular disease It is possible to reduce the portion while strengthening the key ingredients, thereby providing a functional red ginseng useful for enhancing certain diseases and functions.

For example, Rg3 in ginsenosides of red ginseng is known to have anticancer activity, platelet aggregation and antithrombotic activity, experimental liver injury, blood vessel relaxation, and anticancer drug resistance. Red ginseng fortified with Rg3 may be prepared by citric acid and ascorbic acid treatment and reduced pressure treatment.

According to the present invention, red ginseng enhanced with a particular ginsenoside may be provided in a product alone, but may be prepared and applied in various formulations such as powder, beverage, tablet, flake, capsule, and syrup. The following describes various formulation examples of red ginseng prepared according to the present invention. However, it will be apparent to those skilled in the art that the following formulation is not intended to limit the present invention.

Formulation example  One. Powder  Produce

According to the preparation method of powder in the pharmacopeia formulation, it is prepared in the following ingredient content per one packet.

Dry powder of red ginseng prepared in Example 4 50 mg

Lactose ......................................... 100 mg

Talc ......................................... 10 mg

Formulation example  2. Preparation of Tablets

According to the preparation method of tablets in the pharmacopeia formulation, it is prepared in the following component content per tablet.

Dry powder of red ginseng prepared in Example 4 .................. 50 mg

Corn starch ..................... 100 mg

Lactose ......................................... 100 mg

Magnesium Stearate ......................................... 2 mg

Formulation example  3. Manufacture of capsule

According to the preparation method of capsules in the pharmacopeia formulation, it is prepared in the following component content per capsule.

Dry powder of red ginseng prepared in Example 4 50 mg

Corn Starch ... 100 mg

Lactose ........................... 100 mg

Magnesium Stearate ............... 2 mg

Formulation example  4. Liquid  Produce

According to the preparation method of the liquid formulation in the Pharmacopoeia General Formulation, it is prepared in the following component content per 100 ml of the liquid formulation.

Dry powder of red ginseng prepared in Example 4 ...................................... 50 mg

Isomerized sugar ......................................... 10 g

Mannitol ......................................... 5 g

Purified water .............................

In addition, the health beverage is prepared as follows.

The dry powder of red ginseng prepared in Example 12 0.1-80%, sugar 5-10%, citric acid 0.05-0.3%, caramel 0.005-0.02%, vitamin C 0.1-1% of the additives were mixed and 79-94% Mixed with purified water to make syrup, and the syrup was sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and dried with specially processed red ginseng by injecting 0.5 to 0.82% of carbon dioxide. A carbonated beverage containing an extract is prepared.

In addition, the instant disinfection was carried out by homogeneously mixing the dry powder with the subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%). After that, it is packaged in small packaging containers such as glass bottles and plastic bottles to produce a healthy beverage.

The composition ratio of the above-mentioned vitamin and mineral mixtures is composed of relatively suitable ingredients for health functional foods in a preferred embodiment, but the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose. After mixing the above components according to a conventional health food production method, and then to prepare a granule, it can be used for producing a health functional food composition according to a conventional method.

As described in detail above, according to the present invention, by reducing the organic acid under reduced pressure and preparing red ginseng, the effect of strengthening a specific ginsenoside in the red ginseng according to the organic acid treatment can be enhanced. By maximizing the manufacturing cost of ginsenoside-enhanced red ginseng can be reduced. In addition, unlike the prior art, the present invention can manufacture ginseng and red ginseng with enhanced functions of ginsenosides, and thus can be easily used for distribution, sale, and processing, and only by enjoying ginseng and red ginseng itself. It is possible to manufacture excellent products that can obtain anti-cancer treatment effects.

Claims (12)

Ginsenoside-enhanced ginseng production method comprising the step of depressurizing the ginseng precipitated in the organic acid solvent for 5 to 30 minutes. The method of claim 1, wherein the organic acid solvent is selected from the group consisting of acetic acid, citric acid, lactic acid, oxalic acid, malic acid and ascorbic acid. Ginsenoside-enhanced ginseng production method characterized in that. The method according to claim 1, wherein the concentration of the organic acid solvent is 0.5-2 M. Depressurizing the ginseng precipitated in the organic acid solvent for 5 to 30 minutes; And ginseng-treated red ginseng comprising the step of increasing the ginseng under reduced pressure. The method according to claim 4, wherein the red ginseng treatment a) steaming the pressure-reduced ginseng for 2 to 3 hours at a temperature of 94 ℃ ~ 100 ℃; b) cooling the steamed ginseng to room temperature; c) drying the cooled ginseng for 1 day to 2 days in a hot air dryer at 50 to 80 ° C .; And d) a method for producing ginsenoside-enhanced red ginseng, comprising the step of drying at a temperature of 40-60 ° C. for 2-5 days. The method of claim 4, wherein the organic acid solvent is selected from the group consisting of acetic acid, citric acid, lactic acid, lactic acid, oxalic acid, malic acid and ascorbic acid. Method for producing red ginseng with enhanced ginsenosides. The method according to claim 5, wherein the concentration of the organic acid solvent is 0.1-2 M. Ginsenoside-enhanced red ginseng, characterized in that the ginseng precipitated in the organic acid solvent for 5 to 30 minutes under reduced pressure, the ginseng treated by the reduced pressure ginseng. The method of claim 8, wherein the organic acid solvent is selected from the group consisting of acetic acid, citric acid, lactic acid, lactic acid, oxalic acid, malic acid and ascorbic acid. Red ginseng with enhanced ginsenosides. Ginseng precipitated in the organic acid solvent for 5 to 30 minutes under reduced pressure, ginsenoside-enhanced red ginseng obtained by the red ginseng treatment of the pressure-reduced ginseng and a food acceptable additive Health supplement food. The method of claim 10, wherein the organic acid solvent is selected from the group consisting of acetic acid, citric acid, lactic acid, lactic acid, oxalic acid, malic acid and ascorbic acid. Health supplements made with. The dietary supplement of claim 10 or 11, wherein the dietary supplement is a formulation selected from the group consisting of powders, beverages, tablets, flakes, capsules, and syrups.

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