WO2015005554A1 - Composition for promoting hair sprouting and hair growth - Google Patents

Composition for promoting hair sprouting and hair growth Download PDF

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Publication number
WO2015005554A1
WO2015005554A1 PCT/KR2014/000749 KR2014000749W WO2015005554A1 WO 2015005554 A1 WO2015005554 A1 WO 2015005554A1 KR 2014000749 W KR2014000749 W KR 2014000749W WO 2015005554 A1 WO2015005554 A1 WO 2015005554A1
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Prior art keywords
hair growth
ginseng extract
composition
hair
weight
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PCT/KR2014/000749
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French (fr)
Korean (ko)
Inventor
홍용덕
김수나
서정아
김세현
정현정
강병하
배우리
신송석
박영호
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(주)아모레퍼시픽
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Publication of WO2015005554A1 publication Critical patent/WO2015005554A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/21—Plant extracts
    • A23V2250/2124—Ginseng
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/14—Extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Definitions

  • the content described herein relates to a ginseng extract with increased content of ginsenosides and a composition for promoting hair growth and hair growth which promotes hair growth by shortening the period of transition from the resting phase to the growth phase.
  • the hair cycle is the growth phase (anagen) of hair growth, the catagen, which is the end of growth and shrinking of the hair follicles, the talogen, which is the time when the nipples stop working and the hair stays in the scalp.
  • the catagen which is the end of growth and shrinking of the hair follicles
  • the talogen which is the time when the nipples stop working and the hair stays in the scalp.
  • the anagen stage (2-7 years) is the period of hair growth, which is divided into the stages of hair production from which hair exits from the hair follicles and the formation of hard keratin in the hair follicles. Hair continues to grow self-development.
  • the Cataract Stage (2-3 weeks) is the period at which the metabolic process slows down while the growth phase ends, maintaining the shape of the hair. This stage does not produce keratin.
  • the degenerative stage accounts for 1% of all hair. At this time, the hair follicle contracts and is divided into hair papilla and surrounded by hair follicles and rises upwards, and cell division is at rest.
  • the Talogen Stage (3 months) is caused by the shrinking of the nipples, the hair follicles gradually growing, and the hair roots being pushed upwards. The period of hair loss is 3-4 months until the next growth stage begins.
  • Alopeci a has a lot of hair in the resting phase, and hair loss is visible. As hair loss progresses, the growth period is shorter, which results in smaller and smaller hairs. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting phase to go to the growth phase quickly and to increase the shortened growth phase.
  • Androgenetic alopecia is caused by a male hormone called testosterone, which is called 5 alpha-reductase.
  • testosterone which is called 5 alpha-reductase.
  • DHT Dihydrotestosterone
  • the method of inhibiting the production of DHT by 5 alpha-reductase is mainly used to treat male alopecia.
  • Gynecologic alopecia is mainly caused by a decrease in the amount of estrogen after menopause.
  • Minoxidil and estrogen are mainly used as treatments for gynecomastia.
  • Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition.
  • Alopecia areata is fundamentally different from androgenetic alopecia, and the treatment is also different, using a method of treating corticosteroids, applying minoxidil to the affected area, or artificially causing irritation in the affected area.
  • the present invention is ginseng extract with increased content of ginsenosides, and promotes hair growth and hair growth with safety while promoting the growth of hair by shortening the period of transition from the resting phase to the growth phase comprising the same It aims to provide the composition for this.
  • One embodiment of the present invention provides a ginseng extract containing at least 2.5% by weight ginsenoside Rb2, at least 3% by weight of ginsenoside Rc and at least 2% by weight of ginsenoside Rgl. .
  • an embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re in the ginseng extract.
  • Another embodiment of the present invention is a manufacturing method of the ginseng extract
  • the ginseng extract obtained by the above step is dissolved in water and extracted with an organic solvent to remove the organic solvent layer, and the water layer is extracted with an organic solvent again provides a method for producing a ginseng extract comprising the step of producing a ginseng extract. .
  • Another embodiment of the present invention provides a composition for promoting hair growth and hair growth comprising a ginseng extract with an increased content of ginsenosides as described above.
  • Hair growth and hair growth promoting composition according to the present invention by containing a large amount of ginsenosides Rb2, Rc and Rgl or Rb2, Rc, Rgl and Re in an increased concentration, it can exhibit a hair growth and hair growth promoting effect of the prior art hair regrowth agent have.
  • Figure 1 is a structural formula showing the chemical structure of each type of ginsenoside according to an embodiment of the present invention.
  • Figure 2 is a graph of HPLC analysis of ginsenosides contained in the ginseng extract according to an embodiment of the present invention.
  • Figure 3 is an HPLC analysis graph of ginsenosides contained in the general ginseng extract of the comparative example of the present invention.
  • Figure 4 is a graph showing the increase in the expression of hair growth factor VEGF in the dermal papilla cells of ginseng extract (Example 1), Comparative ginseng extract (Comparative Example 1) according to an embodiment of the present invention.
  • Example 5 is a ginseng extract (Example 1) according to an embodiment of the present invention, the dermal papilla cell proliferation effect (hDPc prol i ferat ion) of the general ginseng extract (Comparative Example 1) of the comparative example It is a graph showing.
  • Figure 6 is a graph showing the hair follicle dermal papilla cell proliferation effect (hDPc prol i ferat ion) according to the type of ginsenoside of the ginseng extract according to an embodiment of the present invention.
  • FIG. 7 is a diagram showing the hair growth promoting effect of the ginseng extract (Example 1), the general ginseng extract of the Comparative Example (Comparative Example 1) in the human hair follicle organ according to an embodiment of the present invention.
  • An embodiment of the present invention provides a ginseng extract comprising ginsenoside Rb2 of 2.5% by weight, ginsenoside Rc of 3% by weight, and ginsenoside Rgl of 2% by weight or more based on the total weight of ginseng extract. .
  • An embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re with respect to the ginseng extract.
  • Ginsenoside is a type of saponin, a type of glycoside, derived from the roots, stems, leaves, bark and seeds of plants.
  • Ginsenosides include various types of ginsenosides, for example, Rbl, Rb2, Rc, Rd, Re, and Rgl, and exist depending on plant type, cultivation conditions, processing conditions, extraction methods, and ginseng sites. Ginsenosides differ in type and content.
  • Figure 1 shows the chemical structure of each ginsenoside.
  • Ginsenoside can be divided into PPT (Re, Rgl) and PPD (Rbl, Rb2, Rc, Rd) according to its structure, and Rbl, Rb2, Rc, Rd, Re and Rgl are natural ginseng, that is, red ginseng It is a water-soluble substance that is not denatured with sugars removed through ignition.
  • Another embodiment of the present invention provides a ginseng extract extracted from the root of the ginseng portion as the ginseng extract as described above. Specifically, it provides a ginseng extract extracted from the ginseng cup root (misam) of high yield of ginsenosides.
  • each of ginsenosides ginsenoside Rb2 may be included in an amount of 2.5% or more based on the total weight of ginseng extract, ginsenosides Rc may be included in an amount of 3% by weight or more, and ginsenosides Re. May be included in the amount of more than 6% by weight, and ginsenoside Rgl may be included in the amount of 2% by weight or more. All. Ginsenoside Rb2 2.5 to 4.5% by weight, Rc 3 to 7.5% by weight 3 ⁇ 4>, Re may be provided with a ginseng extract containing 6 to 20% by weight and Rgl 2 to 5% by weight 3 ⁇ 4>.
  • ginsenoside Rb2 is added by 2.5% by weight, Rc by 3% by weight, Re by 6% by weight and Rgl by 2% by weight with respect to the total amount of ginseng extract as described above.
  • the ginseng extract can be prepared by using any method that can extract ginsenosides and the like from ginseng. For example, it can be manufactured by a pressure circulation type extraction process, a solvent fractionation method, etc. which alternately perform a pressurization process or a pressure reduction process, and these can also be performed continuously. At this time, the pressure-circulating extraction process may be specifically carried out by adding water, an organic solvent or a mixture thereof to the ginseng and circulating the pressure and depressurization at regular intervals.
  • the present invention can be prepared by the following method ginseng extract having a high content of ginsenosides Rb2, Rc and Rgl as an embodiment.
  • the ginseng extract obtained by the above step is dissolved in water, extracted with an organic solvent, the organic solvent layer is removed, and the ginseng extract may be prepared by a method comprising the step of extracting the water layer with an organic solvent again.
  • the type of solvent that can be used as one embodiment of the present invention is not particularly limited, and may be, for example, water, an organic solvent, or a mixture of water and an organic solvent every day.
  • the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, and chloroform.
  • the mixed solvent of water and the organic solvent may have a ratio of 10 to 90% (v / v) of the organic solvent. More specifically, the solvent may be an extract extracted using 10 to 90% (v / v) ethanol, more specifically 70% (v / v) ethane.
  • the pressurized and reduced pressure extraction process according to an embodiment of the present invention can be extracted for a total of 1.5 to 2.5 hours while sequentially performing the pressurized and reduced pressure extraction process for 20 to 40 minutes with a pressure circulation type extraction device, respectively.
  • the pressure at the time of pressurized extraction according to the embodiment of the present invention is 1 to 3 kgf / cm 2
  • the pressure at the reduced pressure to be 550-650 ⁇ Hg
  • the extraction silver is 65 to 85 ° C.
  • the ginseng extract in general ginseng extract contains 1.5 to 2% by weight of the total ginsenosides, more than 10 times the total content of ginsenosides in the ginseng extract, more specifically 15 ⁇ It can be improved to 25% by weight.
  • One embodiment of the present invention by providing a composition for promoting hair growth and hair growth comprising the ginseng extract as described above can effectively shorten the cycle to the growth phase from the resting phase of the hair growth cycle, general ginseng extract Compared to the case of hair growth, the hair growth promoting effect is significantly higher . great. Therefore, it is possible to achieve the effect of promoting excellent hair growth and hair growth that did not exist before.
  • the present invention provides a hair growth and hair growth promoting purpose of the ginseng extract described in the specification of the present invention as described above, or a hair growth and hair growth promoting use of the composition comprising the ginseng extract.
  • another embodiment of the present invention provides a method for promoting hair growth and hair growth comprising administering to the subject an effective amount of ginseng extract described in the specification of the present invention as described above, wherein the ginseng extract It can be administered in a composition.
  • another embodiment of the present invention may provide the ginseng extract described in the specification of the present invention as described above for use in promoting hair growth and hair growth, and the ginseng extract may be used in the composition.
  • the composition is not particularly limited to the amount of ginseng extract Ginsenosides Rb2, Rc and Rgl, for example, may comprise 2% by weight or more based on the total weight of the composition. For example, it may include 2 to 20% by weight. If the ginseng extract is included in less than 2% by weight can not expect a sufficient hair growth effect, if it contains more than 20% by weight is difficult to manufacture safety or formulation.
  • the composition according to the embodiment of the present invention may be, for example, a pharmaceutical composition, cosmetic composition or health food composition.
  • the pharmaceutical composition for promoting hair growth and hair growth according to an embodiment of the present invention is a preservative, stabilizer, hydrating or emulsifying accelerator, pharmaceutical supplements such as salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances And may be formulated in various oral or parenteral dosage forms according to conventional methods.
  • the oral dosage form may include, for example, tablets, pills, hard and soft accelerators, liquids, suspensions, emulsifiers, syrups, powders, powder granules, granules, pellets, and the like.
  • Surfactants, diluents e.g. lactose, dextrose, sucrose, manni, sorbbi, cellulose and glycine
  • lubricants e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols
  • Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gel latin, tragacanth methylcellose, sodium carboxymethylcellose and polyvinyl pyrrolidine, optionally starch, agar, alginic acid Or pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners such as sodium salts thereof.
  • the tablets can be prepared by conventional mixing, granulating or coating methods.
  • the parenteral dosage form may be a transdermal dosage form, for example, an injection, drop, ointment, lotion, gel, cream, spray, suspension, emulsion, suppository, patch, or the like. It may be, but is not limited thereto.
  • the pharmaceutical composition according to an embodiment of the present invention may be administered parenterally, rectally, topically, transdermally, subcutaneously, etc.
  • the pharmaceutical composition according to an embodiment of the present invention may be administered topically to the scalp, for example. .
  • the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug depends on various factors such as less progression, onset, age, health condition, complications, etc.
  • the hair growth and hair growth promoting composition according to an embodiment of the present invention may be a cosmetic composition, the appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. It is suitable for topical application in all formulations, for example by dispersing the oil phase in solutions, gels, solids, pasty anhydrides and water phases. In the form of emulsions, suspensions, microemulsions, microcapsules, microgranules, or ionic (liposomal) and nonionic vesicle dispersants, or in the form of creams, skins, lotions, powders, ointments, sprays or concealed sticks. Can be provided. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
  • the cosmetic composition according to an embodiment of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, It may be formulated into a cosmetic such as cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream ⁇ body oil and body essence.
  • the formulation of the present invention is a paste, cream or gel, animal fiber plant fiber, wax, paraffin starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component.
  • a carrier component such as silicone, bentonite, silica, talc or zinc oxide.
  • lactose When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, especially in the case of spray, additionally chlorofluorohydrocarbon, Propellants such as propane / butane or dimethyl ether may be included.
  • a solvent, a solvating agent or an emulsifying agent is used as a carrier component, for example, water ethane, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan.
  • liquid carrier diluents such as water, ethane or propylene glycol, suspensions such as polyoxyethylene sorbitan esters and polyoxyethylene sorbitan esters, as the carrier component, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
  • the formulation of the present invention is a surfactant-containing cleansing
  • a carrier component Aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoether, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty Acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • the content of the active ingredient is not particularly limited, but may include an extract including the active ingredient in an amount of 0.001 to 20% by weight based on the total weight of the composition . have. If the content is satisfied it can exhibit excellent efficacy without side effects.
  • Cosmetic composition according to an embodiment of the present invention in addition to the extract containing the active ingredient.
  • Functional additives and ingredients included in general cosmetic compositions may be further included.
  • the functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.
  • ком ⁇ онент In addition to the said functional additive, you may mix
  • ком ⁇ онент In addition to fabric it must maintain as blending components are components, a moisturizing agent, emollient agent, surfactant, active agent, organic and inorganic pigments, organic powder, ultraviolet absorbent, antiseptic, fungicide, antioxidant, plant extract, pH adjuster, alcohol, colorant flavoring the , Blood circulation accelerators, cold depressants, restriction agents, purified water and the like.
  • composition for promoting hair growth and hair growth is an external preparation for skin
  • the external preparation for skin may include any of cosmetics and medicines of various formulations as a generic term, which may be included in the application of external skin. Can be.
  • the hair growth and hair growth promoting composition may be a health food composition, ginsenoside extract containing ginsenoside Rb2, Rc, Re, Rgl as an active ingredient It preferably comprises 0.001 to 20% by weight relative to the total weight of the composition.
  • the extract is less than 0.0% by weight, the efficacy does not appear sufficiently, and when the extract exceeds 20% by weight, the efficiency of the efficacy due to the addition of the active ingredient is reduced.
  • the composition may be a liquid or solid formulation, tablets, capsules, soft capsules, pills, and Granules, beverages (drinks), diet bars, chocolate, caramel formulations or confectionary formulations, and the formulation is not particularly limited.
  • the health food composition of the present invention the health food composition may contain an excipient, sugars, flavorings, pigments, fats and oils, proteins and the like as appropriate in addition to the active ingredient.
  • Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 20 g of 50% ethane was added to 2 g of the obtained ginseng powder, and the extract was pressurized and depressurized by a pressure-circulating extractor for 30 minutes, and then extracted for 2 hours. At this time, the pressure of the pressurization weight release was 2kgf / cm 2 , the pressure during the decompression extraction was set to 600 ⁇ 50 ⁇ Hg and the extraction temperature was set to 75 ° C.
  • the ginseng extract obtained by the said process was filtered, the supernatant was dried under reduced pressure and 0.57 g of dry weight was obtained. Then, the dried product was dissolved in water, extracted with ethyl acetate to remove the ethyl acetate layer, the water layer was extracted with butanol and dried under reduced pressure to obtain a dry weight of 2.7g. This was referred to as Example 1.
  • Example 1 ⁇ Also, after analyzing the ginsenosides contained in the ginseng extract of Example 1 with the following analytical instruments and conditions, the results are shown graphically. And from this result, the content of each component of ginsenosides contained in the extract is analyzed and shown in Table 2 and FIG.
  • Example 1 of the present invention is 2.91% by weight of ginsenoside Rb2 of 2.5% by weight or more, 3.14% by weight of Rc of 3% by weight or more, and 6% by weight of Re. 6.23% by weight and Rgl was found to include more than 2.0% by weight 2. 1% by weight.
  • Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 2 g of the ginseng powder obtained was extracted with 100% of 50% ethane at 75 ° C. for 2 hours, and then the sample was filtered, and the supernatant was dried under reduced pressure to obtain a dry weight of 0.30 g. This was set as Comparative Example 1.
  • the ginsenosides contained in the ginseng extract of Comparative Example 1 were analyzed by HPLC with the same analyzer and conditions as in Example 1, and the results are shown graphically. And from this result, the content of each component of ginsenoside contained in the extract is analyzed and shown in Table 3 and FIG.
  • Comparative Example 1 contains 0.33% by weight ginsenoside Rb2, 0.55 Rc, 0.16% by weight Re and 0.14% by weight Rgl, Example of the present invention It was confirmed that 1 contains about 5 to 15 times more content of each ginsenoside than the general ginseng extract. In addition, it can be seen that the total weight of ginsenosides contained in the ginseng extract also has a significantly higher value than Example 1 of Comparative Example 1.
  • VEGF Vascular endothelial growth factor
  • DPC Dulbecco's modified Eagle's medium
  • FBS fetal bovine serum
  • Example 1 and Comparative Example 1 were each treated with 20 ppm.
  • DMS0 was used as a negative control.
  • the mixed solution treated with Example 1 or Comparative Example 1 was collected ( ' soup collect ' ), and ⁇ GFGF EL ISA (Vascular endothelial growth factor Enzyme-Linked Immunosorbent Assay; R & D biosystems) was compared with Example 1 and Comparative Example. The difference in the expression level according to the ginseng extract of 1 was confirmed. And the result is shown in FIG.
  • Comparative Example 1 a general ginseng extract did not show a significant effect difference compared to the control group did not use ginseng extract.
  • Example 1 of the present invention increased the expression of the hair growth factor VEGF by about three times or more when used in the same concentration as Comparative Example 1. This is because the ginseng extract of Example 1 contains more ginsenosides Rb2, Rc and Rgl than the general ginseng extract of Comparative Example 1, thereby improving the expression of hair growth factor VEGF in dermal papilla cells. It is because it increased excessively. Therefore, it can be seen that the ginseng extract according to the present invention has excellent hair growth promoting ability.
  • the keratin proteins that make up hair are produced in hair root keratinocytes, which are differentiated from dermal papilla cells. Therefore, if the ginseng extract according to the present invention promotes the activity of the dermal papilla cells, it can promote the activity of keratinocytes differentiated therefrom and further promote hair production.
  • H Liman dermal papilla cell cells were used.
  • the cell line was incubated for 24 hours in an incubator maintained at 5% CO 2 , 37 ° C with Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gai thersburg, MD, USA) containing 10% bovine serum serum (FBS).
  • DMEM Dulbecco's modified Eagle's medium
  • FBS bovine serum serum
  • the ginseng extract of Example 1 was then treated with ⁇ and 20 ⁇ ] ⁇ .
  • the ginseng extract of Comparative Example 1 was treated with 20 ppm, respectively.
  • DMS0 was used as a negative control. Twenty four hours after treatment with the test substance, cell proliferation (3 ⁇ 4 ») was measured using the WST-1 kit (Roche). The results are shown in FIG.
  • Example 1 the ginseng extract according to the present invention significantly increased the proliferation of both cells 10Lppm and 20ppm.
  • Example 1 olol treatment showed about 1.5 times higher growth rate of dermal papilla cells than that of Comparative Example 1 which is a general ginseng extract.
  • the ginseng extract according to the present invention can promote the growth of the dermal papilla cells than the conventional ginseng extract, it can also promote the activity and hair production of keratinocytes more effectively.
  • ginsenosides Rbl, Rb2, Rc, Rd, Re, and Rgl which are the main constituents of ginseng extract, in the same manner as in Test Example 2, respectively, were added to the dermal papilla cells by 0.1. ⁇ M, iM was treated, and cell proliferation (3 ⁇ 4>) was measured using the WST-1 kit (Roche) after 24 hours. The results are shown in FIG.
  • the follicular organs were separated one by one from the posterior scalp tissue of a 55-year-old male.
  • William's E medium L—Glutamine (2 mM), Insulin (10 // g / ml), Hydrocortisone (40 ng / ml), Antibiotics (1%) and antifungal agent ().
  • the hair follicles grown at 3 days after the culture were cut and cut into 3 mm, and the selected hair follicle tissues were treated with no drug treatment, and the experimental group was treated with 2 ppm and 5 ppm of ginseng extract obtained in Example 1, respectively. And Comparative Example 1 was divided into 5ppm. Then, 8 days after the treatment, the length measurement and photographing were performed. The results are shown in FIG.
  • ginsenosides of ginsenosides Rbl, Rc, Rgl showed a distinct hair growth promoting effect. All of these are statistically significant.
  • the hair growth in the hair follicle organs treated with 5ppm ginseng extract showed a hair growth promoting effect better than Comparative Example 1 and the control group treated with the same concentration.
  • the case of 1 showed that the average growth length was reduced compared to the control.
  • the ginseng extract according to the present invention includes a ginsenoside Rbl, Rc, and Rgl having an increased content than the conventional ginseng extract in an increased content. Therefore, it can be seen that the effect of promoting the length of hair growth in hair follicles is excellent. Examples of the formulation of the composition according to the present invention will be described below, but can also be used in various other forms, which are intended to explain in detail only, not intended to limit the present invention.
  • the ointment was prepared in a conventional manner according to the composition described in Table 6.
  • the lotion was prepared by a conventional method according to the composition described in Table 7.
  • 50 mg of extract of Example 1, 80 ⁇ 140mg L-carnitine, 180mg soybean oil, palm oil 2mg, vegetable hardened oil ig, 4kg and lecithin 6mg were mixed and layered by 400mg per capsule according to a conventional method to prepare a soft shell cell .
  • Example 1 50 mg of the extract of Example 1, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed drier, and 6 mg of sugar ester was added thereto and tableted with a tableting machine.
  • Example 1 50 mg of the extract of Example 1, 250 mg of anhydrous glucose and 550 mg of starch were mixed, molded into granules using a fluidized bed granulator, and then layered on a fabric to prepare a 'granule.
  • Example 1 50 mg of the extract of Example 1, 10 g of glucose, 0.6 g of citric acid, and liquid Loligosaccharide 2 were mixed, and 300 ml of purified water was added thereto, and 200 ml of each bottle was layered. After filling the bottle sterilized for 4 to 5 seconds at 130 ° C to prepare a beverage drinks.

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Abstract

A composition for promoting hair sprouting and hair growth according to the present invention contains a higher content of ginsenoside Rb2, Rc, and Rg1, and thus can provide better hair sprouting and hair growth effects compared to hair growth agents manufactured by existing techniques. Also, the composition is made very safe by using plant-based natural products and thus can be used as a skin preparation for external application for promoting hair sprouting and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions, and food compositions, among others.

Description

【명세서】  【Specification】
【발명의 명칭】  [Name of invention]
발모 및 육모 촉진용 조성물  Hair growth and hair growth promoting composition
【기술분야】  Technical Field
본 명세서에 기재된 내용은 진세노사이드의 함량이 증가된 인삼 추출물 과 이를 포함하는 휴지기에서 성장기로 이행되는 주기를 단축시켜 모발의 성장 을 촉진시키는 발모 및 육모 촉진용 조성물에 관한 것이다.  The content described herein relates to a ginseng extract with increased content of ginsenosides and a composition for promoting hair growth and hair growth which promotes hair growth by shortening the period of transition from the resting phase to the growth phase.
【배경기술】  Background Art
최근 사회적 스트레스의 증가와 더불어 환경오염 및 인스턴트 식품 등 서구화된 식습관， 찾은 파마와 염색 등으로 인하여 탈모 인구가 점차 증가하고 있다. 모발의 주기는 모발을 성장시키는 성장기 (anagen) , 성장을 종료하고 모 구부가 축소하는 시기인 퇴화기 (catagen) , 모유두가 활동을 멈추고 모발을 두 피에 머무르게 하는 시기인 휴지기 (talogen) , 모유두가 활동을 시작하거나 또 는 새로운 모발을 발생시켜 오래된 모발을 탈모시키는 시기인 발생기로 나눌 수 있다.  Recently, hair loss population is gradually increasing due to the increase of social stress, westernized eating habits such as environmental pollution and instant food, and found perm and dyeing. The hair cycle is the growth phase (anagen) of hair growth, the catagen, which is the end of growth and shrinking of the hair follicles, the talogen, which is the time when the nipples stop working and the hair stays in the scalp. Can be divided into generators, which are periods of starting activities, or developing new hair and causing the hair to become old.
성장기 (Anagen Stage ; 2~7년)는 모발이 성장하는 기간으로， 다시 모발이 모구로부터 모포로 나가려는 모발 생성 단계와 딱딱한 케라틴이 모낭 안에서 만들어지는 단계로 나누어진다. 모발은 퇴행기까지 자가성장을 계속한다. 퇴행 기 (Catagen Stage ; 2~3주)는 성장기가 끝나고 모발의 형태를 유지하면서 대사 과정이 느려지는 시기로, 이 단계에서는 케라틴을 만들어내지 않는다. 퇴화기 는 전체 모발의 1%를 차지한다. 이때 모구부가 수축하여 모유두로 나워지며 모 낭에 둘러싸여 위쪽으로 올라가고， 세포분열은 정지상태이다. 휴지기 (Talogen Stage ; 3개월)는 모유두가 위축되고 모낭이 차츰 £그라들며, 모근이 위쪽으로 밀려 을라가 빠진다. 모발이 없어지는 시기로서 다음 성장기 단계가 시작될 때 까지의 수명은 3~4개월이다.  The anagen stage (2-7 years) is the period of hair growth, which is divided into the stages of hair production from which hair exits from the hair follicles and the formation of hard keratin in the hair follicles. Hair continues to grow self-development. The Cataract Stage (2-3 weeks) is the period at which the metabolic process slows down while the growth phase ends, maintaining the shape of the hair. This stage does not produce keratin. The degenerative stage accounts for 1% of all hair. At this time, the hair follicle contracts and is divided into hair papilla and surrounded by hair follicles and rises upwards, and cell division is at rest. The Talogen Stage (3 months) is caused by the shrinking of the nipples, the hair follicles gradually growing, and the hair roots being pushed upwards. The period of hair loss is 3-4 months until the next growth stage begins.
정상인 사람이 성장기 상태의 모발이 많은데 비해 탈모증 (Alopeci a)인 사람은 휴지기 상태의 모발이 많아 눈으로 보이는 탈모현상이 나타내게 된다. 탈모가 진행될수록 성장기의 기간이 짧아지고 이로 인해 모발은 점점 소형화된 다. 따라서， 탈모의 치료를 위해서는 휴지기 상태의 모낭을 성장기로 빨리 갈 수 있도록 하고， 짧아진 성장기를 늘려주는 것이 중요하다.  Alopeci a has a lot of hair in the resting phase, and hair loss is visible. As hair loss progresses, the growth period is shorter, which results in smaller and smaller hairs. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting phase to go to the growth phase quickly and to increase the shortened growth phase.
남성형 탈모증은 테스토스테론 (Testosterone)이라는 남성호르몬에 의해 나타나는 현상으로 이 테스토스테론이 5 알파 -리덕타아제 ( a— reductase)라는 효소에 의해 더 강력한 호르몬인 디히드로테스토스테론 (Dihydrotestosterone , DHT)으로 바뀌게 되면， 이 호르몬이 모낭에 작용하여 모낭을 성장기 단계에서 퇴화기 단계로 유도하여 탈모가 일어나게 한다. 따라서， 남성형 탈모증을 치료 하기 위하여 5 알파-리덕타아제에 의한 DHT의 생성을 억제하는 방법이 주로 사 용된다. Androgenetic alopecia is caused by a male hormone called testosterone, which is called 5 alpha-reductase. When the enzyme is converted to a more powerful hormone, Dihydrotestosterone (DHT), it acts on the hair follicles, causing hair follicles from the growth stage to the degenerative stage, causing hair loss. Therefore, the method of inhibiting the production of DHT by 5 alpha-reductase is mainly used to treat male alopecia.
여성형 탈모증은 주로 폐경기 이후 에스트로겐 양의 감소에 의해 발생한 다. 여성형 탈모증을 위한 치료제로는 주로 미녹시딜이나 에스트로겐이 사용되 고 있다.  Gynecologic alopecia is mainly caused by a decrease in the amount of estrogen after menopause. Minoxidil and estrogen are mainly used as treatments for gynecomastia.
원형 탈모증은 자가면역질환이나 정신적 스트레스， 유전적 소인에 의해 발생한다. 이러한 원형 탈모증은 안드로겐성 탈모증과는 근본적으로 원인이 다 르며, 치료법 또한 달라서 부신피질 호르몬제를 처리하는 방법을 사용하거나, 미녹시딜을 환부에 바르거나 인위적으로 환부에 자극을 유발하는 방법을 사용 한다.  Alopecia areata is caused by autoimmune diseases, mental stress, or genetic predisposition. Alopecia areata is fundamentally different from androgenetic alopecia, and the treatment is also different, using a method of treating corticosteroids, applying minoxidil to the affected area, or artificially causing irritation in the affected area.
이처럼 다양하고 복잡한 탈모 원인에 대하여， 혈액 순환 촉진， 남성 호 르몬 작용 억게, 모근 기능 강화 등을 목적으로 하는 성분 등이 시중에 제품으 로 판매되고 있으나， 뚜렷한 효과를 지닌 것은 아직까지 없는 상태이고 부작용 의 문제가 제기되기도 한다. 예를 들어， 미녹시딜의 경우 끈적이는 사용감과 피부에 자극을 유발하는 부작용이 보고된 바 있으며， 피나스테라이드의 경우 현재 경구투여용 제제로 사용되고 있으나ᅳ 이의 섭취에 따라 성기능 장애 등의 부작용이 보고되었을 뿐 아니라， 경구복용에 의해서만 효과를 기대할 수 있어 서 사용상에 많은 불편함이 있었다.  As for the various and complex causes of hair loss, ingredients for the purpose of promoting blood circulation, suppressing male hormone action, and strengthening the hair root function are available on the market, but there are still no clear effects and side effects. Some questions are raised. For example, minoxidil has been reported to cause sticky feeling and irritation to the skin, and finasteride is currently used as an oral preparation, but side effects such as sexual dysfunction have been reported according to its intake. The effect can be expected only by oral administration, there was a lot of inconvenience in use.
[선행기술문헌] [Preceding technical literature]
[특허문헌] [Patent Documents]
한국특허공개 제 1으2011-0000433호  Korean Patent Publication No. 1 2011-0000433
【발명의 상세한 설명】  [Detailed Description of the Invention]
【기술적 과제】  [Technical problem]
상기와 같은 문제점을 해결하기 위하여， 본 발명은 진세노사이드의 함량 이 증가된 인삼 추출물과, 이를 포함하는 휴지기에서 성장기로 이행되는 주기 를 단축시켜 모발의 성장을 촉진시키면서 안전성을 갖춘 발모 및 육모 촉진용 조성물을 제공하는 것을 그 목적으로 한다.  In order to solve the above problems, the present invention is ginseng extract with increased content of ginsenosides, and promotes hair growth and hair growth with safety while promoting the growth of hair by shortening the period of transition from the resting phase to the growth phase comprising the same It aims to provide the composition for this.
【기술적 해결방법】  Technical Solution
상기와 같은 목적을 해결하기 위하여， 본 발명의 일 실시예는 인삼 추출물 총 중량에 대하여 진세노사이드 Rb2 를 2.5 중량 % 이상, 진세노사이드 Rc를 3 중량 % 이상 및 진세노사이드 Rgl을 2 중량 ¾ 이상으로 포함하는 인삼 추출물을 제공한다. In order to solve the above object, One embodiment of the present invention provides a ginseng extract containing at least 2.5% by weight ginsenoside Rb2, at least 3% by weight of ginsenoside Rc and at least 2% by weight of ginsenoside Rgl. .
또한， 본 발명의 일 실시예는 상기 인삼 추출물에 진세노사이드 Re를 6 중량 % 이상으로 더 포함하는 인삼 추출물을 제공한다.  In addition, an embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re in the ginseng extract.
본 발명의 다른 일 실시예는 상기 인삼 추출물의 제조방법으로，  Another embodiment of the present invention is a manufacturing method of the ginseng extract,
인삼에 물， 유기용매 또는 물 및 유기용매의 흔합물을 가한 후, 가압 및 감압 추출공정을 순차적으로 반복 실시하면서 추출하는 단계 ;및  Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting while repeatedly performing pressure and pressure extraction processes; and
상기 단계에 의해 얻어진 인삼 추출물을 물에 녹인 후 유기용매로 추출 하여 유기용매 층을 제거하고， 물 층을 다시 유기용매로 추출하여 인삼 추출물 을 제조하는 단계를 포함하는 인삼 추출물의 제조방법을 제공한다.  The ginseng extract obtained by the above step is dissolved in water and extracted with an organic solvent to remove the organic solvent layer, and the water layer is extracted with an organic solvent again provides a method for producing a ginseng extract comprising the step of producing a ginseng extract. .
본 발명의 또 다른 일 실시예는 상기와 같이 진세노사이드의 함량이 증 가된 인삼 추출물을 포함하는 발모 및 육모 촉진용 조성물을 제공한다.  Another embodiment of the present invention provides a composition for promoting hair growth and hair growth comprising a ginseng extract with an increased content of ginsenosides as described above.
【유리한 효과】  Advantageous Effects
본 발명에 따른 발모 및 육모 촉진용 조성물은 진세노사이드 Rb2 , Rc 및 Rgl 또는 Rb2 , Rc , Rgl 및 Re를 증가된 농도로 다량 포함함으로써， 종래기술에 의한 발모제 이상의 발모 및 육모 촉진 효과를 나타낼 수 있다.  Hair growth and hair growth promoting composition according to the present invention by containing a large amount of ginsenosides Rb2, Rc and Rgl or Rb2, Rc, Rgl and Re in an increased concentration, it can exhibit a hair growth and hair growth promoting effect of the prior art hair regrowth agent have.
또한, 식물성 천연물을 사용하기 때문에 안전성이 우수하므로 발모 및 육모 촉진을 위한 피부 외용제로 사용할 수 있으며， 화장료 조성물， 약학 조성 물 및 식품 조성물 등에 널리 사용될 수 있다.  In addition, since the use of vegetable natural products is excellent in safety, it can be used as an external preparation for promoting hair growth and hair growth, and can be widely used in cosmetic compositions, pharmaceutical compositions and food compositions.
【도면의 간단한 설명】  [Brief Description of Drawings]
도 1은 본 발명의 일 실시예에 따른 진세노사이드의 종류별 각 화학구조 를 보여주는 구조식이다.  Figure 1 is a structural formula showing the chemical structure of each type of ginsenoside according to an embodiment of the present invention.
도 2는 본 발명의 일 실시예에 따른 인삼 추출물에 함유된 진세노사이드 의 HPLC 분석 그래프이다.  Figure 2 is a graph of HPLC analysis of ginsenosides contained in the ginseng extract according to an embodiment of the present invention.
도 3은 본 발명의 비교예의 일반 인삼 추출물에 함유된 진세노사이드의 HPLC 분석 그래프이다.  Figure 3 is an HPLC analysis graph of ginsenosides contained in the general ginseng extract of the comparative example of the present invention.
도 4는 본 발명의 일 실시예에 따른 인삼 추출물 (실시예 1) , 비교예의 일반 인삼 추출물 (비교예 1)의 모유두 세포에서 모발 성장인자 VEGF의 발현 증 가를 보여주는 그래프이다.  Figure 4 is a graph showing the increase in the expression of hair growth factor VEGF in the dermal papilla cells of ginseng extract (Example 1), Comparative ginseng extract (Comparative Example 1) according to an embodiment of the present invention.
도 5는 본 발명의 일 실시예에 따른 인삼 추출물 (실시예 1) , 비교예의 일반 인삼 추출물 (비교예 1)의 모유두 세포 증식효과 (hDPc prol i ferat ion)를 보여주는 그래프이다. 5 is a ginseng extract (Example 1) according to an embodiment of the present invention, the dermal papilla cell proliferation effect (hDPc prol i ferat ion) of the general ginseng extract (Comparative Example 1) of the comparative example It is a graph showing.
도 6은 본 발명의 일 실시예에 따른 인삼추출물의 주요 구성성분인 진세 노사이드 종류별 모낭 모유두 세포 증식 효과 (hDPc prol i ferat ion)를 보여주는 그래프이다.  Figure 6 is a graph showing the hair follicle dermal papilla cell proliferation effect (hDPc prol i ferat ion) according to the type of ginsenoside of the ginseng extract according to an embodiment of the present invention.
도 7은 본 발명의 일 실시예에 따른 인삼 추출물 (실시예 1)， 비교예의 일반 인삼 추출물 (비교예 1)의 인체 모낭기관에서의 모발 성장 촉진 효과를 보 여주는 도이다.  7 is a diagram showing the hair growth promoting effect of the ginseng extract (Example 1), the general ginseng extract of the Comparative Example (Comparative Example 1) in the human hair follicle organ according to an embodiment of the present invention.
【발명의 실시를 위한 최선의 형태】  [Best form for implementation of the invention]
이하， 본 발명을 상세하게 설명한다. 본 발명의 일 실시예는 인삼 추출물 총 중량에 대하여 진세노사이드 Rb2 를 2.5 증량 % 이상， 진세노사이드 Rc를 3 중량 % 이상， 진세노사이드 Rgl을 2 중량 % 이상으로 포함하는 인삼 추출물을 제공한다.  Hereinafter, the present invention will be described in detail. An embodiment of the present invention provides a ginseng extract comprising ginsenoside Rb2 of 2.5% by weight, ginsenoside Rc of 3% by weight, and ginsenoside Rgl of 2% by weight or more based on the total weight of ginseng extract. .
본 발명의 일 실시예는 상기 인삼 추출물에 대하여 진세노사이드 Re를 6 중량 % 이상으로 더 포함하는 인삼 추출물을 제공한다.  An embodiment of the present invention provides a ginseng extract further comprising 6% by weight or more of ginsenoside Re with respect to the ginseng extract.
진세노사이드 (ginsenoside)란 글리코사이드 (glycoside)의 일종인 사포닌 의 한 종류로서， 식물의 뿌리， 줄기， 잎， 껍질， 씨 등에서 유래된다. 진세노사 이드에는 예를 들어 진세노사이드 Rbl , Rb2 , Rc , Rd, Re , Rgl 등의 다양한 종 류가 존재하며, 식물의 종류와 재배조건， 가공 조건， 추출방법， 인삼의 부위 등에 따라 존재하는 진세노사이드의 종류 및 함량이 다르다. 도 1에는 상기 각 진세노사이드의 화학구조를 도시하여 나타내었다.  Ginsenoside is a type of saponin, a type of glycoside, derived from the roots, stems, leaves, bark and seeds of plants. Ginsenosides include various types of ginsenosides, for example, Rbl, Rb2, Rc, Rd, Re, and Rgl, and exist depending on plant type, cultivation conditions, processing conditions, extraction methods, and ginseng sites. Ginsenosides differ in type and content. Figure 1 shows the chemical structure of each ginsenoside.
진세노사이드는 그 구조에 따라 PPT(Re , Rgl) , PPD(Rbl , Rb2 , Rc , Rd)로 나눌 수 있으며， Rbl , Rb2 , Rc , Rd, Re 및 Rgl는 자연인삼 그대로의 조성, 즉 홍삼화를 통한 당이 제거된 변성이 이루어지지 않은 극성의 물질로서 수용성이 다.  Ginsenoside can be divided into PPT (Re, Rgl) and PPD (Rbl, Rb2, Rc, Rd) according to its structure, and Rbl, Rb2, Rc, Rd, Re and Rgl are natural ginseng, that is, red ginseng It is a water-soluble substance that is not denatured with sugars removed through ignition.
본 발명의 다른 일 실시예는 상기와 같은 인삼 추출물로 인삼 부위 중 뿌리로부터 추출한 인삼 추출물을 제공한다. 구체적으로， 상기 진세노사이드의 수율이 높은 인삼 잔뿌리 (미삼)로부터 추출한 인삼 추출물을 제공한다.  Another embodiment of the present invention provides a ginseng extract extracted from the root of the ginseng portion as the ginseng extract as described above. Specifically, it provides a ginseng extract extracted from the ginseng cup root (misam) of high yield of ginsenosides.
본 발명의 일 실시예로서 상기 진세노사이드는 각각 진세노사이드 Rb2는 인삼 추출물 총 중량에 대하여 2.5 중량 이상으로 포함될 수 있으며, 진세노 사이드 Rc는 3 중량 % 이상으로 포함될 수 있고， 진세노사이드 Re는 6 증량 % 이 상으로 포함될 수 있으며, 진세노사이드 Rgl은 2 중량 % 이상으로 포함될 수 있 다. 진세노사이드 Rb2을 2.5~4.5 중량 % , Rc을 3~7.5 중량 ¾>, Re를 6~20 중량 % 및 Rgl을 2~5 중량 ¾>로 포함하는 인삼 추출물을 제공할 수 있다. As an embodiment of the present invention, each of ginsenosides ginsenoside Rb2 may be included in an amount of 2.5% or more based on the total weight of ginseng extract, ginsenosides Rc may be included in an amount of 3% by weight or more, and ginsenosides Re. May be included in the amount of more than 6% by weight, and ginsenoside Rgl may be included in the amount of 2% by weight or more. All. Ginsenoside Rb2 2.5 to 4.5% by weight, Rc 3 to 7.5% by weight ¾>, Re may be provided with a ginseng extract containing 6 to 20% by weight and Rgl 2 to 5% by weight ¾>.
본 발명의 일 실시예에 따르면, 상기와 같은 인삼 추출물 총 증량에 대 하여 진세노사이드 Rb2을 2.5 증량 % 이상， Rc을 3 증량 % 이상， Re를 6 중량 % 및 Rgl을 2 중량 % 이상으로 포함하는 인삼 추출물은 인삼으로부터 진세노사이 드 등의 성분을 추출할 수 있는 방법이라면 제한되지 않고 사용하여 제조할 수 있다. 예를 들어 가압공정 또는 감압 공정을 교대로 수행하는 압력 순환형 추 출공정, 용매분획법 등으로 제조할 수 있으며, 이들을 연속적으로 실시할 수도 있다. 이때 압력 순환형 추출공정은 구체적으로 인삼에 물， 유기용매 또는 이 들의 흔합물을 가한 후 일정 시간마다 가압 및 감압을 순환하면서 실시함으로 써 추출할 수 있다.  According to an embodiment of the present invention, ginsenoside Rb2 is added by 2.5% by weight, Rc by 3% by weight, Re by 6% by weight and Rgl by 2% by weight with respect to the total amount of ginseng extract as described above. The ginseng extract can be prepared by using any method that can extract ginsenosides and the like from ginseng. For example, it can be manufactured by a pressure circulation type extraction process, a solvent fractionation method, etc. which alternately perform a pressurization process or a pressure reduction process, and these can also be performed continuously. At this time, the pressure-circulating extraction process may be specifically carried out by adding water, an organic solvent or a mixture thereof to the ginseng and circulating the pressure and depressurization at regular intervals.
보다 구체적으로， 본 발명은 일 실시예로서 상기와 같은 높은 진세노사 이드 Rb2 , Rc 및 Rgl의 함량을 갖는 인삼 추출물을 하기와 같은 방법으로 제조 할 수 있다.  More specifically, the present invention can be prepared by the following method ginseng extract having a high content of ginsenosides Rb2, Rc and Rgl as an embodiment.
인삼에 물， 유기용매 또는 물 및 유기용매의 흔합물을 가한 후， 가압 및 감압 추출공정을 순차적으로 순환 실시하면서 추출하는 단계 ;및  Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting while sequentially performing pressure and pressure extraction processes; and
상기 단계에 의해 얻어진 인삼 추출물을 물에 녹인 후 유기용매로 추출 하고 유기용매 층을 제거한 다음， 물 층을 유기용매로 다시 추출하는 단계를 포함하는 방법에 의해 인삼 추출물을 제조할 수 있다.  The ginseng extract obtained by the above step is dissolved in water, extracted with an organic solvent, the organic solvent layer is removed, and the ginseng extract may be prepared by a method comprising the step of extracting the water layer with an organic solvent again.
구체적으로 본 발명의 일 실시예로서 사용 가능한 용매의 종류는 특별히 제한되는 것은 아니며， 예를 들어 물， 유기용매， 또는 물과 유기용매의 흔합용 매일 수 있다. 예를 들어, 상기 유기용매는 에탄올， 메탄올， 부탄올， 에테르， 에틸아세테이트 및 클로로포름으로 이루어진 군으로부터 선택되는 하나 이상일 수 있다. 예를 를면 상기 물과 유기용매의 흔합용매는 유기용매의 비가 10 내 지 90%(v/v)일 수 있다. 보다 구체적으로 상기 용매는 10 내지 90%(v/v) 에탄 올， 보다 구체적으로는 70%(v/v) 에탄을을 이용하여 추출된 추출물일 수 있다.  Specifically, the type of solvent that can be used as one embodiment of the present invention is not particularly limited, and may be, for example, water, an organic solvent, or a mixture of water and an organic solvent every day. For example, the organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, and chloroform. For example, the mixed solvent of water and the organic solvent may have a ratio of 10 to 90% (v / v) of the organic solvent. More specifically, the solvent may be an extract extracted using 10 to 90% (v / v) ethanol, more specifically 70% (v / v) ethane.
또한， 본 발명의 일 실시예에 따르면 상기 가압 및 감압 추출공정은 압 력순환형 추출장치로 가압 및 감압 추출공정을 각각 20~40분 동안 순차적으로 순환 실시하면서 총 1.5~2.5시간 동안 추출할 수 있다. 그리고 본 발명의 일 실시예에 따른 가압추출시의 압력은 1~3 kgf/cm2 , 감압추출시의 압력은 550-650 隱 Hg로 하였으며 추출 은도는 65~85°C이다. In addition, the pressurized and reduced pressure extraction process according to an embodiment of the present invention can be extracted for a total of 1.5 to 2.5 hours while sequentially performing the pressurized and reduced pressure extraction process for 20 to 40 minutes with a pressure circulation type extraction device, respectively. have. In addition, the pressure at the time of pressurized extraction according to the embodiment of the present invention is 1 to 3 kgf / cm 2 , the pressure at the reduced pressure to be 550-650 隱 Hg, and the extraction silver is 65 to 85 ° C.
상기와 같이 감압 및 가압 반복 순환공정을 실시할 경우， 인삼의 열변성 을 최소화하여 진세노사이드 변성， 예를 들면 진세노사이드 Rbl에서 Rg3로 당 분해되는 것을 최소화할 수 있다. 따라서, 인삼 추출물의 기존의 단순한 추출 장치로 반복없이 추출할 경우에 비해 추출수율을 25~30% 향상시킬 수 있다. 또 한， 이 후 에틸아세테이트와 같은 유기용매를 사용하여 비극성 물질을 제거하 고, 부탄을과 같은 유기용매로 물 층을 추출함으로써 진세노사이드의 함량을 극대화할 수 있다. 구체적으로 이와 같은 용매 분획법에 의하면， 일반 인삼추 출물에는 총 진세노사이드가 1.5~2 중량 %가 함유된 것에 비하여 인삼 추출물 내 진세노사이드의 총 함량을 기존대비 10배 이상， 구체적으로 15~25중량 %로 향상시킬 수 있다. Thermal degeneration of ginseng in case of repeated pressure reduction and pressurization By minimizing the ginsenoside denaturation, for example, it is possible to minimize the sugar breakdown to Rg3 in the ginsenoside Rbl. Therefore, the extraction yield of the ginseng extract can be improved by 25 to 30% compared to the case of extracting without repeating the conventional simple extraction device. In addition, it is possible to maximize the content of ginsenosides by removing the nonpolar material using an organic solvent such as ethyl acetate and extracting the water layer with an organic solvent such as butane. Specifically, according to the solvent fractionation method, the ginseng extract in general ginseng extract contains 1.5 to 2% by weight of the total ginsenosides, more than 10 times the total content of ginsenosides in the ginseng extract, more specifically 15 ~ It can be improved to 25% by weight.
본 발명의 일 실시예는 상기와 같은 인삼 추출물을 포함하는 발모 및 육 모 촉진용 조성물을 제공함으로써 모발의 성장주기 중 휴지기에서 성장기로 이 행되는 주기를 효과적으로 단축시켜 줄 수 있으므로, 일반 인삼 추출물을 포함 하는 경우에 비하여 모발의 성장 촉진효과가 현저히. 우수하다. 따라서 기존에 없었던 뛰어난 발모 및 육모를 촉진하는 효과를 달성할 수 있다. One embodiment of the present invention by providing a composition for promoting hair growth and hair growth comprising the ginseng extract as described above can effectively shorten the cycle to the growth phase from the resting phase of the hair growth cycle, general ginseng extract Compared to the case of hair growth, the hair growth promoting effect is significantly higher . great. Therefore, it is possible to achieve the effect of promoting excellent hair growth and hair growth that did not exist before.
일 실시예에 따르면 본 발명은 상기와 같이 본 발명의 명세서에 기재된 인삼 추출물의 발모 및 육모 촉진용도， 또는 상기 인삼 추출물을 포함하는 조 성물의 발모 및 육모 촉진용도를 제공한다.  According to one embodiment the present invention provides a hair growth and hair growth promoting purpose of the ginseng extract described in the specification of the present invention as described above, or a hair growth and hair growth promoting use of the composition comprising the ginseng extract.
또한， 본 발명의 다른 일 실시예는 상기와 같이 본 발명의 명세서에 기 재된 인삼 추출물을 유효성분으로써 대상에 유효량으로 투여하는 것을 포함하 는 발모 및 육모 촉진 방법을 제공하며， 이때 상기 인삼 추출물을 조성물에 포 함되어 투여될 수 있다.  In addition, another embodiment of the present invention provides a method for promoting hair growth and hair growth comprising administering to the subject an effective amount of ginseng extract described in the specification of the present invention as described above, wherein the ginseng extract It can be administered in a composition.
나아가， 본 발명의 또 다른 일 실시예는 발모 및 육모 촉진에 사용하기 위하여 상기와 같이 본 발명의 명세서에 기재된 인삼 추출물을 제공할 수 있으 며， 이때 상기 인삼 추출물을 조성물에 포함하여 사용할 수 있다.  Furthermore, another embodiment of the present invention may provide the ginseng extract described in the specification of the present invention as described above for use in promoting hair growth and hair growth, and the ginseng extract may be used in the composition.
본 발명의 일 실시예에 따르면， 상기 조성물은 상기 진세노사이드 Rb2 , Rc 및 Rgl을 포함하는 인삼 추출물의 양을 특별히 한정하지는 않지만, 예를 들 어 조성물 총 중량에 대하여 2중량 % 이상 포함할 수 있으며, 예를 들면 2 내지 20중량 %로 포함할 수 있다. 인삼 추출물을 2중량 % 미만으로 포함할 경우 충분 한 모발 성장 효과를 기대할 수 없고， 20증량 %을 초과하여 포함할 경우 안전성 또는 제형 상의 제조에 어려움이 있기 때문이다.  According to one embodiment of the invention, the composition is not particularly limited to the amount of ginseng extract Ginsenosides Rb2, Rc and Rgl, for example, may comprise 2% by weight or more based on the total weight of the composition. For example, it may include 2 to 20% by weight. If the ginseng extract is included in less than 2% by weight can not expect a sufficient hair growth effect, if it contains more than 20% by weight is difficult to manufacture safety or formulation.
상기 본 발명의 일 실시예에 따른 조성물은 예를 들어, 약학 조성물， 화 장료 조성물 또는 건강식품 조성물일 수 있다. 본 발명의 일 실시예에 따른 상기 발모 및 육모 촉진용 약학 조성물은 방부제， 안정화제, 수화제 또는 유화 촉진제, 삼투압 조절을 위한 염 및 /또는 완충제 등의 약제학적 보조제 및 기타 치료적으로 유용한 물질을 추가로 함유 할 수 있으며， 통상적인 방법에 따라 다양한 경구 투여제 또는 비경구 투여제 형태로 제형화할 수 있다. The composition according to the embodiment of the present invention may be, for example, a pharmaceutical composition, cosmetic composition or health food composition. The pharmaceutical composition for promoting hair growth and hair growth according to an embodiment of the present invention is a preservative, stabilizer, hydrating or emulsifying accelerator, pharmaceutical supplements such as salts and / or buffers for the control of osmotic pressure and other therapeutically useful substances And may be formulated in various oral or parenteral dosage forms according to conventional methods.
상기 경구 투여제는 예를 들면， 정제， 환제， 경질 및 연질 갑셀제， 액 제， 현탁제， 유화제， 시럽제， 분제, 산제 세립제， 과립제， 펠렛제 등이 있으 며， 이들 제형은 유효 성분 이외에 계면 활성제， 희석제 (예: 락토즈, 덱스트로 즈， 수크로즈， 만니를， 솔비를, 셀를로오스 및 글리신)， 활택제 (예: 실리카， 탈크， 스테아르산 및 그의 마그네슘 또는 칼슘염 및 폴리에틸렌 글리콜)를 함 유할 수 있다. 정제는 또한 마그네슘 알루미늄 실리케이트， 전분페이스트， 젤 라틴， 트라가칸스 메틸셀를로오스， 나트륨 카복시메틸셀를로오스 및 폴리비닐 피롤리딘과 같은 결합제를 함유할 수 있으며， 경우에 따라 전분， 한천， 알긴산 또는 그의 나트륨 염과 같은 붕해제， 흡수제, 착색제, 향미제， 및 감미제 등의 약제학적 첨가제를 함유할 수 있다. 상기 정제는 통상적인 흔합, 과립화 또는 코팅 방법에 의해 제조될 수 있다. 또한， 상기 비경구 투여 형태로 경피 투여 형 제형일 수 있으며, 예를 들어 주사제， 점적제， 연고， 로션, 겔, 크림， 스프 레이， 현탁제, 유제, 좌제 (坐劑)， 패취 등의 제형일 수 있으나， 이에 제한되는 것은 아니다.  The oral dosage form may include, for example, tablets, pills, hard and soft accelerators, liquids, suspensions, emulsifiers, syrups, powders, powder granules, granules, pellets, and the like. Surfactants, diluents (e.g. lactose, dextrose, sucrose, manni, sorbbi, cellulose and glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols) ) Can be included. Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gel latin, tragacanth methylcellose, sodium carboxymethylcellose and polyvinyl pyrrolidine, optionally starch, agar, alginic acid Or pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners such as sodium salts thereof. The tablets can be prepared by conventional mixing, granulating or coating methods. In addition, the parenteral dosage form may be a transdermal dosage form, for example, an injection, drop, ointment, lotion, gel, cream, spray, suspension, emulsion, suppository, patch, or the like. It may be, but is not limited thereto.
본 발명의 일 실시예에 따른 상기 약학 조성물은 비경구， 직장， 국소, 경피, 피하 등으로 투여될 수 있다ᅳ 본 발명의 일 실시예에 따른 약학 조성물 은 예를 들어 두피에 국소 투여될 수 있다.  The pharmaceutical composition according to an embodiment of the present invention may be administered parenterally, rectally, topically, transdermally, subcutaneously, etc. The pharmaceutical composition according to an embodiment of the present invention may be administered topically to the scalp, for example. .
상기 유효 성분의 투여량 결정은 당업자의 수준 내에 있으며， 약물의 1 일 투여 용량은 투여하고자 하는 대상의 미만 진행 정도, 발병 시기， 연령， 건 강상태， 합병증 등의 다양한 요인에 따라 달라지지만, 성인을 기준으로 할 때 일반적으로는 상기 조성물 X«g/kg 내지 . 200mg/kg , 바람직하게는 50/ /1 내지 50mg/kg을 1일 1 내지 3회 분할하여 투여할 수 있으며, 상기 투여량은 어떠한 방법으로도 본 발명의 범위를 한정하는 것이 아니다.  Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug depends on various factors such as less progression, onset, age, health condition, complications, etc. Based on the composition generally the composition X «g / kg to. 200 mg / kg, preferably 50 / / 1 to 50 mg / kg may be administered by dividing 1 to 3 times a day, the dosage does not limit the scope of the invention in any way.
본 발명의 일 실시예에 따른 상기 발모 및 육모 촉진용 조성물은 화장료 조성물일 수 있으며， 화장료 조성물의 외형은 화장품학 또는 피부과학적으로 허용 가능한 매질 또는 기제를 함유한다. 이는 국소적용에 적합한 모든 제형으 로， 예를 들면， 용액， 겔， 고체, 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼， 현탁액， 마이크로에멀젼， 마이크로캡술, 미세과립구 또는, 이온 형 (리포좀) 및 비이온형의 소낭 분산제의 형태로, 또는 크림, 스킨， 로션, 파 우더， 연고， 스프레이 또는 콘실 스틱의 형태로 제공될 수 있다. 이들 조성물 은 당해 분야의 통상적인 방법에 .따라 제조될 수 있다. 본 발명에 따른 조성물 은 또한 포말 ( foam)의 형태로 또는 압축된 추진제를 더 함유한 에어로졸 조성 물의 형태로도 사용될 수 있다. The hair growth and hair growth promoting composition according to an embodiment of the present invention may be a cosmetic composition, the appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. It is suitable for topical application in all formulations, for example by dispersing the oil phase in solutions, gels, solids, pasty anhydrides and water phases. In the form of emulsions, suspensions, microemulsions, microcapsules, microgranules, or ionic (liposomal) and nonionic vesicle dispersants, or in the form of creams, skins, lotions, powders, ointments, sprays or concealed sticks. Can be provided. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
본 발명의 일 실시예에 따른 상기 화장료 조성물은 그 제형에 있어서 특 별히 한정되는 바가 없으며， 예를 들면， 유연화장수， 수렴화장수, 영양화장수， 영양크림, 마사지크림, 에센스， 아이크림， 아이에센스， 클렌징크림， 클렌징폼， 클렌징워터， 팩, 파우더， 바디로션， 바디크림ᅳ 바디오일 및 바디에센스 등의 화장품으로 제형화될 수 있다.  The cosmetic composition according to an embodiment of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, It may be formulated into a cosmetic such as cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream ᅳ body oil and body essence.
본 발명의 제형이 페이스트， 크림 또는 겔인 경우에는 담체 성분으로서 동물섬유 식물섬유， 왁스， 파라핀 전분, 트라칸트， 셀를로오스 유도체, 폴리 에틸렌 글리콜， 실리콘, 벤토나이트， 실리카， 탈크 또는 산화아연 등이 이용될 수 있다.  When the formulation of the present invention is a paste, cream or gel, animal fiber plant fiber, wax, paraffin starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. Can be.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락 토스， 탈크， 실리카， 알루미늄히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고， 특히 스프레이인 경우에는 추가적으로 클로로플루오 로히드로카본, 프로판 /부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있 다.  When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, especially in the case of spray, additionally chlorofluorohydrocarbon, Propellants such as propane / butane or dimethyl ether may be included.
본 발명의 제형이 용액 또는 유탁액의 경우에는 담체 성분으로서 용매， 용매화제 또는 유탁화제가 이용되고, 예컨대 물 에탄을， 이소프로판올， 에틸 카보네이트, 에틸 아세테이트， 벤질 알코올， 벤질 벤조에이트, 프로필렌 글리 콜， 1 ,3-부틸글리콜 오일, 글리세를 지방족 에스테르， 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.  In the case of the solution or emulsion of the present invention, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, for example, water ethane, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄을 또는 프로필렌 글리콜과 같은 액상 희석제, 에록실화 이소스테아릴 알코을 폴리옥 시에틸렌 소르비를 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현 탁제, 미소결정성 셀를로오스, 알루미늄 메타히드록시드， 벤토나이트， 아가 또 는 트라칸트 등이 이용될 수 있다. 본 발명의 제형이 계면ᅳ활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코을 에테르 설페이트， 설포숙신산 모노에 스테르, 이세티오네이트， .이미다졸리늄 유도체， 메틸타우레이트， 사르코시네이 트， 지방산 아미드 에테르 설페이트， 알킬아미도베타인， 지방족 알코을， 지방 산 글리세리드， 지방산 디에탄을아미드, 식물성 유， 리놀린 유도체 또는 에특 실화 글리세를 지방산 에스테르 등이 이용될 수 있다. When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethane or propylene glycol, suspensions such as polyoxyethylene sorbitan esters and polyoxyethylene sorbitan esters, as the carrier component, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used. When the formulation of the present invention is a surfactant-containing cleansing, as a carrier component Aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoether, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty Acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
본 발명의 일 실시예에 따르면 유효성분의 함량은 특별히 제한되지 않으 나， 유효성분을 포함하는 추출물을 조성물 총 중량에 대하여 0.001 내지 20 중 량%로 포함할 수. 있다. 상기 함량을 만족하는 경우 부작용없이 우수한 효능을 나타낼 수 있다. According to one embodiment of the present invention, the content of the active ingredient is not particularly limited, but may include an extract including the active ingredient in an amount of 0.001 to 20% by weight based on the total weight of the composition . have. If the content is satisfied it can exhibit excellent efficacy without side effects.
본 발명의 일 실시예에 따른 화장료 조성물에는 상기 유효성분을 포함하 는 추출물 이외에. 기능성 첨가물 및 일반적인 화장료 조성물에 포함되는 성분 이 추가로 포함될 수 있다. 상기 기능성 첨가물로는 수용성 비타.민, 유용성 비 타민， 고분자 펩티드， 고분자 다당， 스핑고 지질 및 해초 액기스로 이루어진 군에서 선택된 성분을 포함할 수 있다.  Cosmetic composition according to an embodiment of the present invention, in addition to the extract containing the active ingredient. Functional additives and ingredients included in general cosmetic compositions may be further included. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.
본 발명의 화장료 조성물에는 또한， 상기 기능성 첨가물과 더불어 필요 에 따라 일반적인 화장료 조성물에 포함되는 성분을 배합해도 된다. 이외에 포 함되는 배합 성분으로서는 유지 성분， 보습제， 에몰리엔트제, 계면' 활성제， 유 기 및 무기 안료, 유기 분체， 자외선 흡수제， 방부제， 살균제， 산화 방지제， 식물 추출물， pH 조정제， 알콜， 색소 향료, 혈행 촉진제, 냉감제， 제한 (制汗) 제， 정제수 등을 들 수 있다. In addition to the said functional additive, you may mix | blend the component contained in a general cosmetic composition with the cosmetic composition of this invention as needed. In addition to fabric it must maintain as blending components are components, a moisturizing agent, emollient agent, surfactant, active agent, organic and inorganic pigments, organic powder, ultraviolet absorbent, antiseptic, fungicide, antioxidant, plant extract, pH adjuster, alcohol, colorant flavoring the , Blood circulation accelerators, cold depressants, restriction agents, purified water and the like.
더욱이， 상기 본 발명의 일 실시예에 따른 발모 및 육모 촉진용 조성물 은 피부 외용제이며, 상기 피부 외용제는 피부 외부에서 도포되는 어떠한 것이 라도 포함될 수 있는 총칭으로서 다양한 제형의 화장품, 의약품이 여기에 포함 될 수 있다.  Furthermore, the composition for promoting hair growth and hair growth according to an embodiment of the present invention is an external preparation for skin, and the external preparation for skin may include any of cosmetics and medicines of various formulations as a generic term, which may be included in the application of external skin. Can be.
또한， 본 발명의 일 실시예에 따른 상기 발모 및 육모 촉진용 조성물은 건강식품 조성물일 수 있으며， 본 발명의 건강식품 조성물은 진세노사이드 Rb2 , Rc , Re , Rgl을 유효성분으로 포함하는 인삼 추출물을 조성물 총 중량에 대하여 0.001 내지 20중량 %를 포함하는 것이 바람직하다. 추출물이 0.0이중량 % 미만인 경우에는 효능이 충분하게 나타나지 않고， 20중량 %를 넘는 경우에는 유 효성분 첨가로 인한 효능의 효율성이 저하된다.  In addition, the hair growth and hair growth promoting composition according to an embodiment of the present invention may be a health food composition, ginsenoside extract containing ginsenoside Rb2, Rc, Re, Rgl as an active ingredient It preferably comprises 0.001 to 20% by weight relative to the total weight of the composition. When the extract is less than 0.0% by weight, the efficacy does not appear sufficiently, and when the extract exceeds 20% by weight, the efficiency of the efficacy due to the addition of the active ingredient is reduced.
본 발명의 일 실시예에 따른 건강식품 조성물에 있어서， 상기 조성물은 액상 또는 고체 상태의 제형일 수 있고， 정제， 캡슐제, 연질캡슐제， 환제, 과 립제， 음료 (드링크제)， 다이어트바， 초콜렛, 카라멜 제형 또는 과자류 제형일 수 있으며， 그 제형이 특별히 한정되는 것은 아니다. 본 발명의 건강식품 조성 물은 건강식품 조성물은 상기 유효 성분 외에도 필요에 따라 부형제， 당류， 향 료， 색소， 유지류， 단백질 등을 적의 함유할 수 있다. In the health food composition according to an embodiment of the present invention, the composition may be a liquid or solid formulation, tablets, capsules, soft capsules, pills, and Granules, beverages (drinks), diet bars, chocolate, caramel formulations or confectionary formulations, and the formulation is not particularly limited. The health food composition of the present invention, the health food composition may contain an excipient, sugars, flavorings, pigments, fats and oils, proteins and the like as appropriate in addition to the active ingredient.
【발명의 실시를 위한 형태】  [Form for implementation of invention]
이하， 본 발명을 하기의 실시예 및 시험예를 통하여 설명한다. 실시예 및 시험예는 본 발명을 보다 상세히 설명하기 위한 것으로 본 발명의 범위가 하기의 실시예의 범위로 제한되는 것은 아니다.  Hereinafter, the present invention will be described through the following examples and test examples. Examples and test examples are intended to explain the present invention in more detail, but the scope of the present invention is not limited to the scope of the following examples.
또한， 이 기술분야의 통상의 지식을 가진 자이면 누구나 이 발명의 기술 사상의 범주를 이탈하지 않고 첨부한 특허청구범위 내에서 다양한 변형 및 모 방이 가능함은 명백한 사실이다.  In addition, it is obvious that any person skilled in the art can make various modifications and imitations within the scope of the appended claims without departing from the scope of the technical idea of the present invention.
[실시예 1] 진세노사이드 Rb2 , Rc , Rgl및 Re의 함량이 증가된 포함하는 인삼 추출물의 제조 Example 1 Preparation of Ginseng Extract Containing Increased Content of Ginsenosides Rb2, Rc, Rgl and Re
인삼 (금산인삼， 구입처: 금산인삼농협)을 정제수로 세척하고 건조시킨 다음 분쇄하여 세말화한 인삼 분말을 얻었다. 얻어진 인삼 분말 2g을 50% 에탄 을을 20 mL 가한 후， 압력순환형 추출장치로 가압 및 감압 추출공정을 각각 30 분 동안 순차적으로 순환 실시하면서 총 2시간 동안 추출하였다. 이때 가압추 출시의 압력은 2kgf /cm2 , 감압추출시의 압력은 600 ± 50 瞧 Hg로 하였으며 추출 온도는 75 °C로 설정하였다. 상기 공정에 의해 얻어진 인삼 추출물을 여과하고, 그 상등액을 감압 건조하여 건조중량 0.57g을 얻었다. 그 다음， 건조물을 물에 녹인 후 에틸아세테이트로 추출하여 에틸아세테이트 층을 제거하고 물층을 부 탄올로 추출 후 감압건조하여 건조중량 2 .7g을 얻었다. 이를 실시예 1로 하였 다. ᅳ 또한， 이 실시예 1의 인삼 추출물에 함유된 진세노사이드를 하기의 분석 기기 및 조건으로 HPLC분석한 후 그 결과를 그래프로 나타내었다. 그리고 이 결과로부터 상기 추출물에 함유된 진세노사이드의 각 성분별 함량을 분석하여 증량 %로 표 2 및 도 2에 나타내었다. Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 20 g of 50% ethane was added to 2 g of the obtained ginseng powder, and the extract was pressurized and depressurized by a pressure-circulating extractor for 30 minutes, and then extracted for 2 hours. At this time, the pressure of the pressurization weight release was 2kgf / cm 2 , the pressure during the decompression extraction was set to 600 ± 50 瞧 Hg and the extraction temperature was set to 75 ° C. The ginseng extract obtained by the said process was filtered, the supernatant was dried under reduced pressure and 0.57 g of dry weight was obtained. Then, the dried product was dissolved in water, extracted with ethyl acetate to remove the ethyl acetate layer, the water layer was extracted with butanol and dried under reduced pressure to obtain a dry weight of 2.7g. This was referred to as Example 1. ᅳ Also, after analyzing the ginsenosides contained in the ginseng extract of Example 1 with the following analytical instruments and conditions, the results are shown graphically. And from this result, the content of each component of ginsenosides contained in the extract is analyzed and shown in Table 2 and FIG.
HPLC분석기기 및 분석초건 HPLC Analysis Equipment and Analysis
기기: Waters 2998 PDA detect er , 1525 pump , 2707 autosamp l er 컬럼: Sun f i re C18 305 μ ιη , 4.6 X150 隱 Instrument: Waters 2998 PDA detect er, 1525 pump, 2707 autosamp l er Column: Sun fi re C18 305 μ ιη, 4.6 X150 隱
검출기 (detector ) : UV 203nm  Detector: UV 203nm
유량 (Flow rate) : lmL/min  Flow rate: lmL / min
주입 부피 ( Inj ect ion vol Limn) : 20 y 1  Injection volume (Inj ect ion vol Limn): 20 y 1
조건 : gradi ent (A-물， B-아세토나이트릴 )  Condition: gradi ent (A-water, B-acetonitrile)
【표 1】 Table 1
Figure imgf000013_0001
Figure imgf000013_0001
【표 2】  Table 2
Figure imgf000013_0002
그 결과， 표 2 및 도 2에 나타난 바와 같이 본 발명의 실시예 1은 진세 노사이드 Rb2를 2.5중량% 이상인 2.91중량 %， Rc를 3중량 % 이상인 3. 14중량 %， Re를 6중량 % 이상인 6.23 중량 % 및 Rgl을 2.0중량 % 이상인 2. 1중량%로 포함하 고 있음을 확인할 수 있었다.
Figure imgf000013_0002
As a result, as shown in Table 2 and FIG. 2, Example 1 of the present invention is 2.91% by weight of ginsenoside Rb2 of 2.5% by weight or more, 3.14% by weight of Rc of 3% by weight or more, and 6% by weight of Re. 6.23% by weight and Rgl was found to include more than 2.0% by weight 2. 1% by weight.
[비교예 1] 일반 인삼 추출물의 제조 Comparative Example 1 Preparation of General Ginseng Extract
인삼 (금산인삼， 구입처: 금산인삼농협)을 정제수로 세척하고 건조시킨 다음 분쇄하여 세말화한 인삼 분말을 얻었다. 얻어진 인삼 분말 2g을 50% 에탄 을 lOOmL로 75°C에서 2시간 동안 추출한 후 시료를 여과하여， 그 상등액을 감 압 건조하여 건조중량 0.30g을 얻었다. 이를 비교예 1로 하였다. Ginseng (Gumsan Ginseng, purchased from Geumsan Ginseng Nonghyup) was washed with purified water, dried and ground to obtain a powdered ginseng powder. 2 g of the ginseng powder obtained was extracted with 100% of 50% ethane at 75 ° C. for 2 hours, and then the sample was filtered, and the supernatant was dried under reduced pressure to obtain a dry weight of 0.30 g. This was set as Comparative Example 1.
또한， 이 비교예 1의 인삼 추출물에 함유된 진세노사이드를 상기 실시예 1과 동일한 분석기기 및 조건으로 HPLC분석한 후 그 결과를 그래프로 나타내었 다. 그리고 이 결과로부터 상기 추출물에 함유된 진세노사이드의 각 성분별 함 량을 분석하여 중량 %로 표 3 및 도 3에 나타내었다.  In addition, the ginsenosides contained in the ginseng extract of Comparative Example 1 were analyzed by HPLC with the same analyzer and conditions as in Example 1, and the results are shown graphically. And from this result, the content of each component of ginsenoside contained in the extract is analyzed and shown in Table 3 and FIG.
【표 3】
Figure imgf000014_0001
그 결과, 표 3 및 도 3에 나타난 바와 같이 비교예 1은 진세노사이드 Rb2를 0.33중량%， Rc를 0.55， Re를 0.16 중량 % 및 Rgl을 0.14중량%로 포함하고 있어， 본 발명의 실시예 1이 일반 인삼 추출물보다 각 진세노사이드를 약 5~15 배 이상의 함량 %로 포함하고 있음을 확인할 수 있었다. 또한 인삼 추출물에 함유된 진세노사이드의 총 중량도 본 발명의 실시예 1이 비교예 1보다 현저히 높은 수치를 가짐을 확인할 수 있다. Table 3
Figure imgf000014_0001
As a result, as shown in Table 3 and Figure 3 Comparative Example 1 contains 0.33% by weight ginsenoside Rb2, 0.55 Rc, 0.16% by weight Re and 0.14% by weight Rgl, Example of the present invention It was confirmed that 1 contains about 5 to 15 times more content of each ginsenoside than the general ginseng extract. In addition, it can be seen that the total weight of ginsenosides contained in the ginseng extract also has a significantly higher value than Example 1 of Comparative Example 1.
[시험예 1] 모유두 세포에서 모발 성장인자 VEGF의 발현 평가 Test Example 1 Expression of Hair Growth Factor VEGF in Hair Papillary Cells
인삼추출물이 혈관형성에 필요한 성장인자로 모발의 성장기로 이동하는 데 필요한 성장인자인 혈관 내피 성장 인자 (Vascular endothelial growth factor; VEGF)를 발현시키는 정도를 평가하기 위해, 생체 외 (in vitro) 시스템 을 적용하여 인삼추출물 적용 후 활성을 평가하였다.  To evaluate the extent to which ginseng extract expresses Vascular endothelial growth factor (VEGF), which is a growth factor required for angiogenesis, to move to the hair growth phase, an in vitro system was developed. The activity was evaluated after the application of ginseng extract.
상기 실험을 위해 47세 남성의 모유두 진피세포 (Dermal Papilla Cell;  Dermal Papilla Cells of 47-year-old Males for the Experiment;
DPC) (P.11) 4 X 105 cell을 12 웰 플레이트 (well plate)에 씨딩 (seeding)하 고， 10% FBS (fetal bovine serum)를 함유하는 DMEM (Dulbecco's modified Eagle's medium) 배지에서 하룻밤 동안 배양하였다. 배양 후， 상기 실시예 1과 비교예 1을 각각 20 ppm로 처리하였다. 음성 대조군으로는 DMS0를 사용하였다. 24시간 후 상기 실시예 1 또는 비교예 1이 처리된 흔합액을 수집 (soup collect)하고' VEGF EL ISA (Vascular endothelial growth factor Enzyme-Linked Immunosorbent Assay; R&D biosystems)를 사용하여 실시예 1과 비교예 1의 인 삼 추출물에 따른 발현 정도의 차이를 확인하였다. 그리고 그 결과를 도 4에 나타내었다. DPC) (P. 11) Seeding 4 × 10 5 cells in a 12 well plate and overnight in Dulbecco's modified Eagle's medium (DMEM) medium containing 10% FBS (fetal bovine serum). Incubated. After incubation, Example 1 and Comparative Example 1 were each treated with 20 ppm. DMS0 was used as a negative control. After 24 hours, the mixed solution treated with Example 1 or Comparative Example 1 was collected ( ' soup collect ' ), and `` GFGF EL ISA (Vascular endothelial growth factor Enzyme-Linked Immunosorbent Assay; R & D biosystems) was compared with Example 1 and Comparative Example. The difference in the expression level according to the ginseng extract of 1 was confirmed. And the result is shown in FIG.
도 4에서 볼 수 있듯이， 일반 인삼 추출물인 비교예 1은 인삼추출물을 사용하지 않은 대조군과 비교하여 큰 효과의 차이가 나타나지 않았다. 하지만， 본 발명의 실시예 1은 비교예 1과 동일 농도로 사용했을 때 모유두 세포에서 모발 성장인자 VEGF의 발현을 약 3배 이상 증가시켰다. 이는 실시예 1의 인삼 추출물이 진세노사이드 Rb2, Rc 및 Rgl 등을 비교예 1의 일반 인삼 추출물보다 더 많이 함유함으로써 모유두 세포에서 모발 성장인자 VEGF의 발현을 보다 효 과적으로 증가시켰기 때문이다. 따라서， 본 발명에 따른 인삼 추출물은 우수한 모발 생성 촉진능을 가짐을 알 수 있다. As can be seen in Figure 4, Comparative Example 1, a general ginseng extract did not show a significant effect difference compared to the control group did not use ginseng extract. However, Example 1 of the present invention increased the expression of the hair growth factor VEGF by about three times or more when used in the same concentration as Comparative Example 1. This is because the ginseng extract of Example 1 contains more ginsenosides Rb2, Rc and Rgl than the general ginseng extract of Comparative Example 1, thereby improving the expression of hair growth factor VEGF in dermal papilla cells. It is because it increased excessively. Therefore, it can be seen that the ginseng extract according to the present invention has excellent hair growth promoting ability.
[시험예 2] 모낭 모유두 세포 증식 효과 평가 [Test Example 2] Evaluation of hair follicle dermal papilla cell proliferation effect
모발을 구성하는 케라틴 단백질은 모근부 케라틴형성세포 (keratinocyte) 에서 생성되며, 이 케라틴형성세포는 모유두 세포로부터 분화된다. 따라서 본 발명에 따른 인삼 추출물이 모유두 세포의 활성을 촉진한다면 그로부터 분화되 는 케라틴형성세포의 활성을 촉진시킬 수 있고， 나아가 모발 생성을 촉진할 수 있을 것이다.  The keratin proteins that make up hair are produced in hair root keratinocytes, which are differentiated from dermal papilla cells. Therefore, if the ginseng extract according to the present invention promotes the activity of the dermal papilla cells, it can promote the activity of keratinocytes differentiated therefrom and further promote hair production.
이에 본 실험에서는 인삼추출물의 모유두 세포 활성 촉진 효과를 하기와 같이 평가하였다.  In this experiment, the effect of promoting ginseng papilla cell activity of ginseng extract was evaluated as follows.
먼저, 본 발명에서는 사람 모유두 세포 (HLiman dermal papilla cell) 세 포주를 사용하였다. 상기 세포주는 10% 소혈청세럼 (FBS)이 함유된 Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gai thersburg, MD, USA)으로 5% C02 , 37°C가 유지되는 인큐베이터에서 24시간 동안 배양한 후 실시예 1의 인삼 추출물을 ΙΟρρι 및 20ρρι]ᅳ .비교예 1의 인삼 추출물을 20ppm로 각각 처리하였 다. 음성 대조군으로는 DMS0를 사용하였다. 시험 물질을 처리한 후 24시간 경 과 후에 WST-1 키트 (Roche)을 사용하여 세포 증식능 (¾»)을 측정하였다. 그 결과 를 도 5에 나타내었다. First, in the present invention, H Liman dermal papilla cell cells were used. The cell line was incubated for 24 hours in an incubator maintained at 5% CO 2 , 37 ° C with Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Gai thersburg, MD, USA) containing 10% bovine serum serum (FBS). The ginseng extract of Example 1 was then treated with ΙΟρρι and 20ρρι] ᅳ. The ginseng extract of Comparative Example 1 was treated with 20 ppm, respectively. DMS0 was used as a negative control. Twenty four hours after treatment with the test substance, cell proliferation (¾ ») was measured using the WST-1 kit (Roche). The results are shown in FIG.
도 5에서 볼 수 있듯이, 본 발명에 따른 인삼추출물인 실시예 1은 lOppm 및 20ppm 모두 두 세포의 증식을 유의차 있게 증가시켰다. 특히， 실시예 1올 lOppm 처리한 경우가 일반 인삼 추출물인 비교예 1을 20ρρτι 처리한 경우보다도 약 1.5배 이상 높은 모유두 세포 증식 증가율을 나타내었다. 이는 본 발명에 따른 인삼 추출물이 기존의 일반 인삼 추출물보다 모유두 세포의 증식을 촉진 시킬 수 있으므로， 케라틴 형성세포의 활성 및 모발 생성도 보다 효과적으로 촉진할 수 있음을 의미한다.  As can be seen in Figure 5, Example 1, the ginseng extract according to the present invention significantly increased the proliferation of both cells 10Lppm and 20ppm. Particularly, Example 1 olol treatment showed about 1.5 times higher growth rate of dermal papilla cells than that of Comparative Example 1 which is a general ginseng extract. This means that the ginseng extract according to the present invention can promote the growth of the dermal papilla cells than the conventional ginseng extract, it can also promote the activity and hair production of keratinocytes more effectively.
[시험예 3] 인체 모낭기관을 이용한 모발 성장 촉진 효과 평가 Test Example 3 Evaluation of Hair Growth Promoting Effect Using Human Hair Follicle Organs
본 발명의 실제 인체 모낭기관에서의 모발 성장 촉진능을 확인한 실험을 실시하였다.  Experiments were conducted to confirm the hair growth promoting ability in the actual human hair follicle organ of the present invention.
먼저， 상기 시험예 2와 동일한 방법으로 인삼 추출물의 주요 구성성분인 진세노사이드 Rbl, Rb2, Rc, Rd, Re 및 Rgl을 각각 종류별로 모유두세포에 0.1 μΜ, iM씩 처리하였고， 24시간 경과 후에 WST-1 키트 (Roche)을 사용하여 세 포 증식능 (¾>)을 측정하였다. 그 결과를 도 6에 나타내었다. First, ginsenosides Rbl, Rb2, Rc, Rd, Re, and Rgl, which are the main constituents of ginseng extract, in the same manner as in Test Example 2, respectively, were added to the dermal papilla cells by 0.1. μM, iM was treated, and cell proliferation (¾>) was measured using the WST-1 kit (Roche) after 24 hours. The results are shown in FIG.
그 다음, 55세 남성의 후두부 두피조직으로부터 모낭 기관을 하나씩 분 리하여 William's E 배지 (L—글투타민 (2mM) , 인술린 (10//g/ml )， 히드로코르티손 (40ng/ml), 항생제 (1%), 항진균제 ( ) 함유)에 배양하였다. 배양 후 3일째에 성장이 일어난 모낭을 선별하여 3mm로 자르고 선별한 모낭 조직에 대해， 약물 을 처리하지 않은 경우를 대조군으로 하고, 실험군은 실시예 1에서 얻은 인삼 추출물 2ppm, 5ppm을 각각 처리한 경우와 비교예 1을 5ppm 처리한 경우로 나누 었다. 그리고， 처리한지 8일 후 각각 길이 측정 및 사진촬영을 진행하였다. 그 결과를 도 7에 나타내었다.  Then, the follicular organs were separated one by one from the posterior scalp tissue of a 55-year-old male. William's E medium (L—Glutamine (2 mM), Insulin (10 // g / ml), Hydrocortisone (40 ng / ml), Antibiotics (1%) and antifungal agent (). The hair follicles grown at 3 days after the culture were cut and cut into 3 mm, and the selected hair follicle tissues were treated with no drug treatment, and the experimental group was treated with 2 ppm and 5 ppm of ginseng extract obtained in Example 1, respectively. And Comparative Example 1 was divided into 5ppm. Then, 8 days after the treatment, the length measurement and photographing were performed. The results are shown in FIG.
그 결과， 도 6에서 볼 수 있듯이 진세노사이드 중 진세노사이드 Rbl, Rc, Rgl이 뚜렷한 모발 성장 촉진 효과를 나타내었다. 이는 모두 통계학적으로 유의미한 것이다.  As a result, as shown in Figure 6 ginsenosides of ginsenosides Rbl, Rc, Rgl showed a distinct hair growth promoting effect. All of these are statistically significant.
또한 도 7에서 볼 수 있듯이, 모낭 기관에서 모발의 길이 증가에 있어서 5ppm의 인삼추출물을 처리한 경우가 동일 농도로 처리한 비교예 1 및 대조군 보다 우수한 모발 성장 촉진 효과가 나타났다. 특히 비교에 1의 경우는 대조군 보다도 평균 성장길이가 감소한 것으로 나타났다.  In addition, as can be seen in Figure 7, the hair growth in the hair follicle organs treated with 5ppm ginseng extract showed a hair growth promoting effect better than Comparative Example 1 and the control group treated with the same concentration. In particular, the case of 1 showed that the average growth length was reduced compared to the control.
따라서 상기 도 6 및 도 7의 결과를 종합해 볼 때， 본 발명에 따른 인삼 추출물은 기존의 일반 인삼 추출물보다 모발 성장 촉진 효과를 갖는 진세노사 이드 Rbl, Rc, Rgl을 더 증가된 함량으로 포함하기 때문에， 모낭에서 모발의 길이 성장을 촉진하는 효과가 우수함을 알 수 있다. 하기에 본 발명에 따른 조성물의 제형예를 설명하나， 다른 여러 가지 제 형으로도 웅용 가능하며， 이는 본 발명을 한정하고자 함이 아닌 단지 구체적으 로 설명하고자 함이다.  Therefore, when combining the results of FIGS. 6 and 7, the ginseng extract according to the present invention includes a ginsenoside Rbl, Rc, and Rgl having an increased content than the conventional ginseng extract in an increased content. Therefore, it can be seen that the effect of promoting the length of hair growth in hair follicles is excellent. Examples of the formulation of the composition according to the present invention will be described below, but can also be used in various other forms, which are intended to explain in detail only, not intended to limit the present invention.
[제형예 1] 피부 외용제 중 샴푸 [Formulation Example 1] Shampoo in the external preparation for skin
하기 표 4에 기재된 조성에 따라 통상적인 방법으로 샴푸를 제조하였다. To prepare a shampoo in a conventional manner according to the composition shown in Table 4.
【표 4】 함량 (중량 %) Table 4 Content (% by weight)
심시예 1의 추출물 2.00 Extract of Sim Axiom 1 2.00
리우림황산나트류 10.00 Liurim Sulfuric Acid Nuts 10.00
코카미도프로 ¾베타?ᅵ 3.00 Cocamido Pro ¾Beta?
카르복심비님폴리머 0.30 Carboxylum Polymer 0.30
폴리춰터뉴 -10 0.20 Policy News -10 0.20
세팀트리메팀암모뉴클로라이드 0. 10 Setim trimethimal ammonium chloride 0. 10
정제수 Purified water
한계 100.00 Limit 100.00
[제형예 2] 피부 외용제 중 린스 [Formulation Example 2] Rinse in external preparations for skin
하기 표 5에 기재된 조성에 따라 통상적인 방법으로 린스를 제조하였다. To prepare a rinse in a conventional manner according to the composition shown in Table 5.
【표 5】 Table 5
서부 함량 (중량^ Western content (weight ^
심시예 1의 추출물 2.00 Extract of Sim Axiom 1 2.00
세팀암콜 2.00 Seteam Amcall 2.00
스테아림일 "콜 2.50 Stearim Days "Call 2.50
베해녘암콜 0.50 Bei Sunset Amcall 0.50
심리콘에멈젼 0.40 Psychocone Stop 0.40
사이큼로메티 s 1.00 Saumromameti s 1.00
디메팀디스테아림암모뉴클로라이드 0. 10 Dimethimistiarim ammonium chloride 0. 10
정체수 잔량 Remaining water
합계 100.00 Total 100.00
[제형예 3] 피부 외용제 중 연고 [Formulation Example 3] Ointment in the external preparation for skin
하기 표 6에 기재된 조성에 따라 통상적인 방법으로 연고를 제조하였다. The ointment was prepared in a conventional manner according to the composition described in Table 6.
【표 6】 Table 6
성분 함량 (중량 %) Ingredient Content (% by weight)
심시예 1의 추출물 2.00 Extract of Sim Axiom 1 2.00
금리세린 8.00 Interest rate 8.00
부팀레그리콜 4.00 Bugteam Legacy 4.00
유동파라휘 15.00 Liquid Parafigure 15.00
베타금루카 7.00 Beta Gold Luca 7.00
카보머 0. 10 Carbomer 0. 10
카프림릭 /카프릭 트리금리세라이드 3.00 Capric Lic / Capric Tri Interest Rate 3.00
스쿠암라 1.00 Scuamla 1.00
세테아림 글루코사이드 1.50 Ceteriarim glucoside 1.50
소르비타 스테아레이트 0.40 Sorbita Stearate 0.40
세테아림 암코올 1.00 Cetearim Amcool 1.00
밀납 4.00 Beeswax 4.00
잔량  Remaining amount
합계 100.00 [제형예 4] 로션 Total 100.00 [Formulation Example 4] Lotion
하기 표 7에 기재된 조성에 따라 통상적인 방법으로 로션을 제조하였다. The lotion was prepared by a conventional method according to the composition described in Table 7.
【표 7】 Table 7
성분 함 : (중량 %) Ingredient box : (wt%)
심시예 1의 추출물 · 2.00. Extract of examination example 1 2.00.
Lᅳ아스코르비산 -2— ᅵ산마그네슈엮 1.00  L ᅳ Ascorbic Acid -2—San Magnesite Lace 1.00
수^성 콜라게 ( 1% 수용액 ) 1.00 Water collagen (1% aqueous solution) 1.00
시트르산나트류 0. 10 Citric acid sodium 10
시트르^^ 0.05 Citrus ^^ 0.05
감초 액기스 0.20 Licorice Extract 0.20
1ᅳ3ᅳ부팀례글리콜 3.00  1 ᅳ 3 ᅳ butymeric glycol 3.00
정제수 잔량 Purified water level
합계 100.00 Total 100.00
[제형예 5] 크림 Formulation Example 5 Cream
하기 표 8에 기재된 조성에 따라 통상적인 방법으로 크림을 제조하였다. To prepare a cream in a conventional manner according to the composition shown in Table 8.
【표 8】 ' [Table 8] '
성분 함량 (중량 ¾ Ingredient Content (Weight ¾
심시예 1의 추출물 2.00 Extract of Sim Axiom 1 2.00
폴리에팀렌긁리콜모노스테아레이트 2.00 Polyimethylene scratching monostearate 2.00
자기유화형 모노스테아르산글리세린 5.00 Self-emulsifying glycerin monostearate 5.00
세팀일 "코올 4.00 Three team " Coal 4.00
스쿠암해 6.00 Scuba Am 6.00
트리 2-에팀핵산금리세림 6.00 Tree 2-Ethymol Nucleic Acid Serum 6.00
스핑고당지침 1 .00 Sphingoe Guidelines 1 .00
1 .3—부팀렌글리콜 7.00  1 .3—butylene glycol 7.00
정제수 잔량 Purified water level
합계 100.00 Total 100.00
[제형예 6] 팩 [Formulation Example 6] Pack
하기 표 9에 기재된 조성에 따라 통상적인 방법으로 팩을 제조하였다. To prepare a pack in a conventional manner according to the composition described in Table 9.
【표 9】 Table 9
성분 함량 (중량 %) Ingredient Content (% by weight)
심시예 1의 추출물 2.00 Extract of Sim Axiom 1 2.00
폴리비녘암코올 13.00 Polynoneum Coal 13.00
L-아스코르빈산 -2-?ᅵ산마그네슴엮 1 .00  L-ascorbic acid-2-? ᅵ Magnetic plait 1 .00
라우로임히드록시프를린 1.00 Lauroim Hydroxyline 1.00
수용성 콜라게 ( 1% 수용액 ) 2.00 Water Soluble Collagen (1% Aqueous Solution) 2.00
1.3-부팀례금리콜 3.00  1.3-Deputy Interest Rate Recall 3.00
에타올 5.00 Ethanol 5.00
정제수 잔량 Purified water level
함계 100.00 [제형예 7] 미용액형 제제 Total 100.00 Formulation Example 7 Cosmetic Liquid Formulation
하기 표 10에 기재된 조성에 따라 통상적인 방법으로 미용액형 제제 제조하였다.  To prepare a cosmetic liquid formulation in a conventional manner according to the composition shown in Table 10.
【표 10】  Table 10
Figure imgf000019_0001
Figure imgf000019_0001
[제형예 8] 연질갑셀제 Formulation Example 8 Soft Chalcedel
실시예 1의 추출물 50mg, Lᅳ카르니틴 80~140mg, 대두유 180mg, 팜유 2mg, 식물성 경화유 ig, 황납 4mg 및 레시틴 6mg을 흔합하고， 통상의 방법에 따라 1캡술당 400mg씩 층진하여 연질갑셀을 제조하였다.  50 mg of extract of Example 1, 80 ~ 140mg L-carnitine, 180mg soybean oil, palm oil 2mg, vegetable hardened oil ig, 4kg and lecithin 6mg were mixed and layered by 400mg per capsule according to a conventional method to prepare a soft shell cell .
[제형예 9] 정제 Formulation Example 9 Tablets
실시예 1의 추출물 50mg, 갈락토올리고당 200mg, 유당 60mg 및 맥아당 140mg을 흔합하고 유동층 건조기를 이용하여 과립한 후 당 에스테르 (sugar ester)를 6mg을 첨가하여 타정기로 타정하여 정제를 제조하였다.  50 mg of the extract of Example 1, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed drier, and 6 mg of sugar ester was added thereto and tableted with a tableting machine.
[제형예 10] 과립제 Formulation Example 10 Granules
실시예 1의 추출물 50mg, 무수결정 포도당 250mg 및 전분 550mg을 흔합 하고, 유동층 과립기를 사용하여 과립으로 성형한 후 포에 층진하여 '과립제를 제조하였다.  50 mg of the extract of Example 1, 250 mg of anhydrous glucose and 550 mg of starch were mixed, molded into granules using a fluidized bed granulator, and then layered on a fabric to prepare a 'granule.
[제형예 11] 드링크제 Formulation Example 11 Drinks
실시예 1의 추출물 50mg, 포도당 10g, 구연산 0.6g, 및 액상 을리고당 2 을 흔합한 후 정제수 300ml를 가하여 각 병에 200ml씩 층진하였다. 병에 충 진한 후 130°C에서 4~5 초간 살균하여 드링크제 음료를 제조하였다. 50 mg of the extract of Example 1, 10 g of glucose, 0.6 g of citric acid, and liquid Loligosaccharide 2 were mixed, and 300 ml of purified water was added thereto, and 200 ml of each bottle was layered. After filling the bottle sterilized for 4 to 5 seconds at 130 ° C to prepare a beverage drinks.

Claims

【청구의 범위】 [Range of request]
【청구항 1】  [Claim 1]
인삼 추출물 총 중량에 대하여 진세노사이드 Rb2를 2.5 중량 % 이상, 진 세노사이드 RC를 3 중량 % 이상 및 진세노사이드 Rgl을 2 중량 % 이상으로 포함 하는 인삼 추출물. A ginseng extract comprising at least 2.5% by weight of ginsenoside Rb2, at least 3% by weight of ginsenoside R C and at least 2% by weight of ginsenoside Rgl.
【청구항 2】  [Claim 2]
제 1 항에 있어서， 상기 인삼 추출물은 진세노사이드 Re를 인삼 추출물 총 중량에 대하여 6 중량 % 이상으로 더 포함하는 것을 특징으로 하는 인삼 추 출물.  The ginseng extract of claim 1, wherein the ginseng extract further comprises ginsenoside Re in an amount of 6% by weight or more based on the total weight of the ginseng extract.
【청구항 3]  [Claim 3]
제 1 항에 있어서， 상기 인삼 추출물은 인삼의 뿌리에서 추출한 것을 특 징으로 하는 인삼 추출물.  The ginseng extract of claim 1, wherein the ginseng extract is extracted from the root of ginseng.
【청구항 4】  [Claim 4]
제 1 항에 있어서, 상기 인삼 추출물은 인삼의 잔뿌리에서 추출한 것을 특징으로 하는 인삼 추출물.  The ginseng extract of claim 1, wherein the ginseng extract is extracted from a small root of ginseng.
【청구항 5】  [Claim 5]
제 1 항의 인삼 추출물의 제조방법으로,  Claim 1 of the manufacturing method of the ginseng extract,
인삼에 물， 유기용매 또는 물 및 유기용매의 흔합물을 가한 후， 가압 및 감압 추출공정을 순차적으로 반복 실시하면서 추출하는 단계 ; 및  Adding water, an organic solvent or a mixture of water and an organic solvent to ginseng, followed by extracting while repeatedly performing a pressurized and reduced pressure extraction step; And
상기 단계에 의해 얻어진 인삼 추출물을 물에 녹인 후 유기용매로 추출 하고 유기용매 층을 제거한 다음, 물 층을 다시 유기용매로 추출하여 인삼 추 출물을 제조하는 단계 ;  Preparing a ginseng extract by dissolving the ginseng extract obtained by the above step in water, extracting it with an organic solvent, removing the organic solvent layer, and then extracting the water layer with an organic solvent again;
를 포함하는 인삼 추출물의 제조방법 .  Method of producing ginseng extract comprising a.
[청구항 6】  [Claim 6]
제 5 항의 제조방법에 의해 제조된 인삼 추출물.  Ginseng extract prepared by the method of claim 5.
【청구항 7]  [Claim 7]
제 1 항 내지 제 4 항 중 어느 한 항의 인삼 추출물을 유효성분으로 포 함하는 발모 및 육모 촉진용 조성물.  Hair growth and hair growth promoting composition comprising the ginseng extract of any one of claims 1 to 4 as an active ingredient.
【청구항 8】  [Claim 8]
제 7 항에 있어서, 상기 인삼 추출물을 조성물 총 중량에 대하여 2 중량 % 이상으로 포함하는 발모 및 육모 촉진용 조성물.  The composition for promoting hair growth and hair growth according to claim 7, wherein the ginseng extract is contained in an amount of 2% by weight or more based on the total weight of the composition.
[청구항 9】 제 7 항에 있어서, 상기 인삼 추출물에 포함되는 진세노사이드는 모발의 성장주기 중 휴지기에서 성장기로 이행되는 주기를 단축시켜주는 것임을 특징 으로 하는 발모 및 육모 촉진용조성물. [Claim 9] 8. The composition for promoting hair growth and hair growth according to claim 7, wherein the ginsenosides included in the ginseng extract shorten the cycle of transition from the resting phase to the growth phase of the hair growth cycle.
【청구항 10】  [Claim 10]
제 7 항에 있어서， 상기 조성물은 약학 조성물인 것을 특징으로 하는 발 모 및 육모 촉진용 조성물.  The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a pharmaceutical composition.
【청구항 11】  [Claim 11]
제 7 항에 있어서， 상기 조성물은 화장료 조성물인 것을 특징으로 하는 발모 및 육모 촉진용 조성물.  8. The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a cosmetic composition.
【청구항 12]  [Claim 12]
제 7 항에 있어서， 상기 조성물은 피부 외용제인 것을 특징으로 하는 발 모 및 육모 촉진용 조성물.  The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is an external preparation for skin.
【청구항 13]  [Claim 13]
제 7 항에 있어세 상기 조성물은 식품 조성물인 것을 특징으로 하는 발 모 및 육모 촉진용 조성물.  The composition for promoting hair growth and hair growth according to claim 7, wherein the composition is a food composition.
PCT/KR2014/000749 2013-07-11 2014-01-27 Composition for promoting hair sprouting and hair growth WO2015005554A1 (en)

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