KR101686008B1 - Composition comprising extract of herbal mixture for preventing alopecia or hair growth effect - Google Patents

Abstract

The present invention relates to a composition containing an extract of a herbal medicine mixture comprising a herbal composition, a lobule, a green leaf, a green tea, and a herbal composition, wherein the composition is excellent in growth and cleavage promotion effect of human dermal papilla cells, A pharmaceutical composition for wool, a health functional food, and a cosmetic composition.

Description

[0001] The present invention relates to a hair-loss-preventing or wool-effective composition containing an extract of a herbal mixture,

The present invention relates to a composition for hair loss prevention or hair wool containing an extract of a herbal medicine mixture comprising a herbal composition, a lobule, a green tea and a herbal composition.

The human hair has about 100,000 to 150,000 hairs, and each hair has a different period and grows and falls through anagen, catagen, and telogen. This cycle repeats over 36 years and results in an average of 50 to 100 hairs per day dropping normally.

In general, alopecia or hair loss means that the number of abnormal hair loss is increased due to the shortening of hair growth rate during the growing period and the increase of hair in the retrograde period or resting period among these cycles. Especially, In the case of male hair loss, there is no decrease in hair density, but it is revealed that hair thickness is extremely reduced.

The most widely used formulation to treat or prevent alopecia is Minoxidil, one of the two FDA-approved hair growth agents. Minoxidil was a remedy for hypertension, but it became a more popular drug as a hair growth drug nowadays with hair growth due to side effects. Minoxidil's hair growth mechanism has not been clarified precisely, but it is supposed that nutrient supply to the hair follicle promotes hair growth by increasing blood flow through vasodilation effect. This model of blood flow increase has been shown indirectly by a recent report that minoxidil increases the expression of vascular endothelial growth factor (VEGF), a growth factor related to vasodilation in the dermal papilla, (Br. J. of Dermatol., 1998, 138, 407-411). In addition, in addition to the vasodilating effect of minoxidil, hair follicle cell activation (Skin Pharmacol., 1996, 9, 3-8) and hair follicle tissue culture promoted hair follicle growth in vivo. Invest. Dermatol., 1989, 92, 315-320) suggest that minoxidil acts as a direct growth factor in hair follicles.

Finasteride, a major component of Merck's recently launched propecia, has also been shown to inhibit the conversion of testosterone to the more potent male hormone dihydrotestosterone (DHT) . In December 1997, a 1 mg tablet was approved for use by the FDA for the treatment of male alopecia, which is currently on the market and has been shown to have significant effects on clinical outcome, but some male functional inhibitory side effects have also been reported (J. Am. Acad. Dermatol , 1998, 39, 578-589). However, the drug is not as excellent as minoxidil, and clinical research is actively pursued for better hair growth due to concerns about side effects.

Although active ingredients that are biased toward each of these effects are reported to be effective in enhancing hair growth in animal experiments or limited clinical trials, they do not meet complex hair growth mechanisms, and the individual hair loss characteristics There are many cases in which the majority of users do not notice a noticeable effect because they are not considered. Therefore, it is necessary to develop new hair growth promoting products that have competitiveness with overseas competitiveness, conceptual competitiveness, and commercialization of original technology.

Houttuynia cordata , also known as anemic medicine , grows in the shaded forests. The underground stalk is long, narrow, thin and white. The stem is straight and 20 ~ 50cm high, has several vertical lines, and smells. Leaves are alternate phyllotaxis, 3 ~ 8cm long, pointed end, plain edge, stipule attached to bottom of petiole. The flower blooms in May to June, and the end of the stem of the stem ends up with a number of spikes on the end of the stem. The involucre is divided into 4 pieces, and under the inflorescence, it looks like a petal with a cruciform shape. The divided pieces are elliptical or elliptical with a length of 1.5 ~ 2cm and white. The length of the inflorescence is 1 ~ 3cm, the flower has no petal and calyx, 3 stamens and 1 pistil, 3 styles, and ovary is upper (upper) and 3 rooms. The fruit is seed vessel, and the seed is light brown. Use the plants before the flowering as a diuretic and insect repellent, and apply the leaves when they are bitten by boils and insects. In the private sector, it is used for swelling, hemorrhoids and hemorrhoids. In one room, the plant is used for gonorrhea, enteritis, urinary tract infection, pneumonia, bronchitis, hypertension, cystitis, sinusitis, feces, exfoliation, malaria and inflammation. Leaves are similar to leaves of buckwheat and are called medicinal plants because they are medicinal plants. It is distributed in Korea (Ulleungdo, Anmyeon-do, Geoje Island), Japan, China, Himalaya and Java. China 's' Prince Gangmok', 'Yeongnam' It is recorded that there is a detoxification effect on the Chinese medicine plant inscription. It is also known that it cleanses blood and improves skin and regenerates.

The leaves of Perilla frutescens var. Acuta [= Perilla sikokiana ] are dried and used. It contains many minerals such as vitamin A, vitamin C, calcium, phosphorus and iron. , Fever, diuretic, dryness, and dementia. In addition, it increases appetite, improves blood circulation, makes sweat better, and has an inhibitory effect such as inflammation and cough. Anemia is reduced, blood cholesterol lowering effect, vascular inflammation inhibition effect, cardiovascular disease prevention effect, etc., and digestive function is good, and headache and athlete's foot is also known to have a therapeutic effect.

Green tea is dried using the leaves of Camellia sinensis . It is heated immediately after collecting the leaves of the tea tree to destroy the oxidizing enzyme to keep the green as it is. At the same time, the water is evaporated and the leaves are faint Previously, a man rubbed leaves directly in a cauldron. Dried green tea leaves continue to be heated to remove most of the moisture to make them crispy to some extent and used as a raw material for tea or herbal medicine. The catechin component of green tea has been known to inhibit various cancers such as gastric cancer, liver cancer, breast cancer, and esophageal cancer. The polysaccharide contained in green tea promotes insulin synthesis and inhibits the rise of blood sugar. It is also known that it is effective in suppressing the aging of the skin due to the heavy metal removal effect, the increase of blood cholesterol, the prevention of obesity and the soothing action of the skin.

Eclipta prostrata grows in rice paddies and wetlands. The lower part grows diagonally and stays straight and smooth, but it has rough hair all over. It is 10 ~ 60cm in height and branches are split in the axilla. Leaves are opposite, 3 ~ 10cm in length, 5 ~ 25mm in width, with hard hairs on both sides and with serrate. Flowers bloom from August to September, and one head flower hangs at the end of the branch and at the end of the main stem. The hare is about 1cm in diameter and the lanceolate is white. It has 5 ~ 6 pieces, green with long oval shape and dull end. The fruit is achene, which grows in black color. The whole pool is made into a medicinal material. It is used as a hemostatic agent in the private sector, especially in the treatment of blood. It is distributed in a warm place in Asia and the world. Hansikcho has a function to protect men's period, and it is known that it has the effect of treating male diseases such as premature ejaculation and erectile dysfunction. It is also effective in improvement of kidney function, lower back pain, women's uterine bleeding, menstrual irregularity and poor circulation. It is also known to inhibit the growth of cancer cells.

On the other hand, the inventors of the present invention have found that when the herbal composition having anti-hair loss or wool effect is studied, the extract of the herbal medicine mixture containing the herbal composition, the lobule, the green tea, By confirming the superiority, the present invention can be completed.

Korean Registered Patent No. 1261321 (Shampoo Composition Comprising Chenopoderm and Preparation Method Thereof, Registered on Mar. 30, 2013.) Korean Registered Patent No. 1322926 (registered trademark of HanSunikcho Extract, its fractions, turtienyl derivatives or pharmaceutically acceptable salts thereof, composition for preventing hair loss and promoting hair growth, registered on October 22, 2013) Korea Patent Publication No. 2011-0112019 (hair cosmetic composition for hair loss prevention and promotion of hair growth and method for producing the same, Korea Patent Publication No. 2011-0104196 (composition for preventing hair loss or promoting hair growth, disclosed on September 22, 2011)

Lachgar, S. et al., Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells. Br. J. of Dermatol., 1998, 138, 407-411. Kurata. S. et al., Effects of hypertrichotic agents on follicular and nonfollicular cells in vitro. Skin Pharmacol., 1996, 9, 3-8. Buhl, A.E. et al., Minoxidil stimulates mouse vibrissae follicles in organ culture. J. Invest. Dermatol., 1989, 92, 315-320. Kaufman K.D. et al., Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group. J. Am. Acad. Dermatol., 1998,39, 578-589. E. Kaiser et al. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides., Anal. Biochem., 1970, 34 (2), 595-598.

It is an object of the present invention to provide a composition for hair loss prevention or hair wool containing an extract of a herbal medicine mixture comprising a herbal composition, a lobule, a green tea and a herbal medicine.

The present invention relates to a composition for preventing hair loss or wool comprising an extract of a herbal medicine mixture comprising 100 parts by weight of herringbone, 20 to 80 parts by weight of a baby leaf, 20 to 80 parts by weight of green tea, and 100 to 300 parts by weight of a herbal medicine.

The extract of the herbal medicine mixture is prepared by mixing the herbal medicine mixture comprising 100 parts by weight of herringbone, 20 to 80 parts by weight of hemp leaflets, 20 to 80 parts by weight of green tea, and 100 to 300 parts by weight of Hansen's syrup with water, C1 to C4 lower alcohol, Followed by extraction with a mixed solvent.

The present invention also provides a hair-dressing preventive or health food for the wool containing an extract of a herbal medicine mixture comprising 100 parts by weight of the herringbone, 20 to 80 parts by weight of a baby leaf, 20 to 80 parts by weight of green tea, and 100 to 300 parts by weight of do.

In still another embodiment, the present invention provides a hair loss preventing or wooly hair treatment composition containing an extract of a herbal medicine mixture comprising 100 parts by weight of herringbone, 20 to 80 parts by weight of a baby leaf, 20 to 80 parts by weight of green tea, and 100 to 300 parts by weight of Thereby providing a cosmetic composition.

The cosmetic composition may further comprise at least one member selected from the group consisting of hair tonic, hair cream, hair lotion, hair shampoo, hair rinse, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, sol gel, emulsion, oil, wax, It may be cosmetics.

Further, the extract of the crude drug mixture of the present invention,

(Step 1): A method for preparing a herbal mixture, comprising mixing 100 parts by weight of an adult plant, 20 to 80 parts by weight of a baby leaf, 20 to 80 parts by weight of a green tea, and 100 to 300 parts by weight of a herbicide.

(Step 2) Extraction of 1 to 10 times the total weight of the crude drug mixture with the herbal mixture in the above-mentioned one step, and extraction at 70 to 100 ° C for 0.5 to 10 hours; And

(Step 3) The step of removing the extract of the extract of the above two steps and collecting the filtrate can be obtained.

The herbal mixture in the above step may be a mixture of 20 to 80% of green lobules, 20 to 80% of green tea, and 100 to 300%

Hereinafter, the present invention will be described in detail.

The herbal medicine mixture used in the present invention may be a mixture of 20 to 80% of self-lobes, 20 to 80 of green tea, and 100 to 300 parts of 100 to 300 parts by weight of Hansikucho on the basis of 100 parts by weight of the perennial herb. The extract of the herbal medicine mixture, Or the wool effect is not good.

The extract of the crude drug mixture can be used by concentrating the extract at a reduced pressure when a C1 to C4 lower alcohol or a mixed solvent with water is used. In addition, in the case of extraction using water, the filtrate from which the mackerel has been removed can be used immediately, and it can also be used in the form of a concentrated liquid under reduced pressure. The C1 to C4 lower alcohols may be selected from one or more of ethanol, methanol, propanol, isopropanol and butanol.

Further, the extraction conditions are such that the extraction solvent is mixed with the herbal mixture at a total weight of 1 to 10 times the weight of the herbal medicine mixture, preferably at 50 to 130 캜, more preferably at 70 to 100 캜, most preferably at 80 to 90 캜 . The extraction time can be preferably extracted for 0.1 to 10 hours, more preferably 0.5 to 10 hours, most preferably 1 to 5 hours.

The extract of the crude drug mixture may be isolated by known methods used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether, acetone, etc.), partition with hexane and water, Or may be fractionated or purified by a suitable combination method.

The chromatography can be carried out using silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, medium pressure liquid chromatography chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

On the other hand, the green tea can be processed green tea leaves that can be drunk by tea, but it is also possible to use fresh green tea leaves in the shade.

According to a preferred embodiment of the present invention, the composition of the present invention may be provided as a pharmaceutical composition or a cosmetic composition.

Examples of the hair to which the pharmaceutical composition or cosmetic composition of the present invention is applied include head hair, eyebrows, eyelashes, pubic hair, underarm hair, chest hair, nose hair, Hair follicles and the like may be included.

The dose (on a dry weight basis) of the extract of the herbal medicine mixture containing the active ingredients of the present invention as the active ingredients is from 0.001 to 1000 mg / kg, preferably from 0.1 to 1000 mg / kg, To 1000 mg / kg, more preferably 1 to 500 mg / kg, and the dose may be administered once a day or divided into several times. The extract (on a dry weight basis) of the crude drug mixture of the present invention is preferably 0.0001 to 50% by weight, more preferably 0.001 to 40% by weight, and most preferably 0.001 to 30% by weight based on the total weight of the pharmaceutical composition. But are not limited thereto.

When the composition of the present invention is prepared from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers are those conventionally used in the field of manufacture and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, But are not limited to, polyvinylpyrrolidone, cellulose, purified water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil and the like. The pharmaceutical composition of the present invention may further include additives commonly used such as lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives and the like in addition to the above components. The pharmaceutical composition of the present invention is preferably oral or parenteral, and is preferably applied topically by parenteral administration. The pharmaceutical compositions may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method have.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on such factors as formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, .

The cosmetic composition of the present invention contains components commonly used in cosmetic compositions, as well as extracts of herbal preparations comprising the active ingredients of the herbicide, the lobule, the green tea and the medicinal herb, such as antioxidants, stabilizers, Vitamins, pigments and flavors, and carriers. Further, the composition of the present invention may be used in combination with a conventional hair-dressing agent (hair growth agent) or an anti-hair-resting agent which has been used so far as it does not impair the hair-loss prevention or wool-like action by the active ingredient of the present invention . As the carrier, there can be used water, purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butylene glycol or propylene glycol), higher fatty acids (palmitic acid or linolenic acid) But are not limited to, oil, camellia oil, jojoba oil, olive oil, squalane, sunflower oil, macadamia peanut oil, avocado oil, or fatty acid glyceride). If necessary, surfactants, moisturizers, preservatives, antioxidants and the like may be added.

Examples of the surfactant that can be used in the cosmetic composition of the present invention include anionic surfactants such as alkylbenzenesulfonates, polyoxyalkylene alkylsulfate ester salts, alkylsulfate ester salts, olefin sulfonates, alkyl phosphates, polyoxyalkylene Alkyl ether phosphates, dialkyl sulfosuccinates, fatty acid salts, and the like. Nonionic surfactants include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyhydric alcohol fatty acid partial esters, polyoxyethylene polyhydric alcohol fatty acid moieties Esters, polyglycerin fatty acid esters, polyoxyethylene hydrogenated castor oil derivatives, and fatty acid diethanolamides. Examples of the cationic surfactant include tertiary aliphatic amine salts, alkyltrimethylammonium halides and dialkyldimethylammonium halides. Examples of the amphoteric surfactants include amide betaine, imidazolinium betaine, sulfobetaine, And the like. Examples of the moisturizing agent include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, and sorbitol. Examples of the preservative include benzoic acid, dehydroacetic acid, paraoxybenzoic acid esters (such as methyl parahydroxybenzoate, butyl parahydroxybenzoate), and phenoxyethanol. Examples of the antioxidant include ascorbic acid and BHA. In addition, an ultraviolet absorber, an anti-inflammatory agent, and a refreshing agent may be added.

The formulations of the cosmetic composition of the present invention may be any formulations that can be combined with the active ingredients of the hair cosmetic composition. Examples of the hair cosmetic composition include hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, Hair gels, emulsions, oils, waxes, aerosols, and the like, such as hair, hair, aerosols, pomades, powders and gels.

The extract (on a dry matter basis) of the herbal medicine mixture comprising the herbal composition, the natural lobule, the green tea and the medicinal herb of the present invention is preferably 0.0001 to 50% by weight, more preferably 0.001 to 40% by weight, But it is not limited thereto.

The cosmetic composition of the present invention can be used everyday or can be used for an unspecified period. Preferably, the amount of usage, the number of times of use, and the period of time can be adjusted according to the user's age, skin condition or skin type, and the concentration of the peptide.

The present invention also provides a hair-loss-preventing or wool-health functional food containing an extract of a herbal medicine mixture comprising the above-mentioned herbal composition, female lobule, green tea, and hanuksikcho. The health functional food includes forms such as tablets, capsules, pills, and liquids. Examples of the foods to which the extract of the present invention can be added include various foods, beverages, gums, tea, Functional foods.

The composition for hair loss prevention or wool, the pharmaceutical composition or the health functional food containing the extract of the herbal medicine mixture of the present invention may be in the form of a dried product or may be used as it is in the form of an extract. If it is used directly in an extractive state, it may be provided as a form which is sterilized packaged in a pouch form and is easily taken or applied to a hair loss region.

The present invention relates to a composition containing an extract of a herbal medicine mixture comprising a herbal composition, a lobule, a green leaf, a green tea, and a herbal composition, wherein the composition is excellent in growth and cleavage promotion effect of human dermal papilla cells, A pharmaceutical composition for wool, a health functional food, and a cosmetic composition.

Korean Patent No. 1261321 discloses a shampoo composition in which green tea is included as a constituent component, Korean Patent No. 1322926 discloses the effect of preventing the hair loss and promoting the hair growth of the myrtle plant extract, and Korean Patent Publication No. 2011-0112019 It is indicated that the fermented product including green tea, hwaseongcho, and hanchikcho can be included in the hair cosmetic for preventing hair loss and promoting hair growth, and Korean Patent Publication No. 2011-0104196 discloses that the hanwoncho contains anti- However, it is not disclosed in the prior art that the extract of the herbal medicine mixture containing all of Hwasungsuja, Liliaceae, Green tea, and Hanshikwoo inhibits hair loss and has a wool effect as in the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a process for producing an extract of a herbal medicine mixture comprising a herbal composition, a lobule, a green tea, and a herb medicine.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the intention is to provide an exhaustive, complete, and complete disclosure of the principles of the invention to those skilled in the art.

<Example 1: Preparation of an extract of crude drug mixture containing Rhizobium, Rhizobia, Green tea,

The crude drug mixture of Table 1 below was mixed with 2 kg of water and extracted with a herbal extractor at 90 ° C for 4 hours. The extract was obtained by removing the mackerel. At this time, the total weight of the crude drug mixture was unified to 400 g. The herbal medicine for the preparation of the extract was dried.

Condition Goshencho (g) Lobulus (g) Green tea (g) Hansik Cho (g) Total (g) Example 1-1 100 50 50 200 400 Examples 1-2 130 70 70 130 400 Example 1-3 100 20 20 260 400 Examples 1-4 100 80 80 140 400 Examples 1-5 100 20 20 260 400

< Comparative Example  1. Preparation of comparative extracts>

The crude drug mixture shown in Table 2 below was mixed with 2 kg of water and extracted with a herbal extractor at 90 ° C for 4 hours. The extracts of each herbal mixture were obtained by removing the mackerel. At this time, the total weight of the crude drug mixture was unified to 400 g.

Condition Goshencho (g) Lobulus (g) Green tea (g) Hansik Cho (g) Total (g) Comparative Example 1-1 400 0 0 0 400 Comparative Example 1-2 0 400 0 0 400 Comparative Example 1-3 0 0 400 0 400 Comparative Example 1-4 0 0 0 400 400 Comparative Example 1-5 200 200 0 0 400 Comparative Example 1-6 200 0 200 0 400 Comparative Example 1-7 200 0 0 200 400 Comparative Example 1-8 0 200 200 0 400 Comparative Example 1-9 130 130 140 0 400 Comparative Example 1-10 130 130 0 140 400 Comparative Example 1-11 130 0 130 140 400 Comparative Example 1-12 100 10 10 280 400 Comparative Example 1-13 100 170 80 50 400 Comparative Example 1-14 10 50 200 140 400

EXPERIMENTAL EXAMPLE 1 Growth and Cleavage Promoting Effect of Human Dermal Papilla Cells [

Human dermal papilla cells (HFDPC) use dermal papilla cells isolated and cultured from human scalp, which is a byproduct of plastic surgery. Human dermal papilla cells were incubated in DMEM (Cambrex) medium containing 10% FBS (Gibco BRL) at 37 ° C and 5% CO ₂ in an incubator.

To treat the extracts of the present invention, human dermal papilla cells were seeded at a constant density of 3,000 cells in a 96-well plate for cell culture and cultured in DMEM medium containing 0.1% FBS for 5 hours at 37 ° C under 5% CO ₂ And incubated in an incubator. The extracts of Example 1 and Comparative Example 1 were lyophilized and then treated in a cell culture medium to a final concentration of 1 μg / ml and then incubated in an incubator at 37 ° C. and 5% CO ₂ for 3 to 5 days. Cells were washed with PBS and chilled 5% TCA and then disrupted with 0.1 N NaOH and 1% SDS (sodium dodecyl sulfate) solution. Cells were disrupted using a liquid scintillation counter (Beckman) The radioactivity was measured and the results are shown in Table 3.

Extract treatment conditions In the control group,
Human dermal papilla cell growth rate (%) Example 1-1 190 Examples 1-2 185 Example 1-3 194 Examples 1-4 213 Examples 1-5 210 Comparative Example 1-1 121 Comparative Example 1-2 135 Comparative Example 1-3 129 Comparative Example 1-4 110 Comparative Example 1-5 121 Comparative Example 1-6 142 Comparative Example 1-7 135 Comparative Example 1-8 128 Comparative Example 1-9 119 Comparative Example 1-10 110 Comparative Example 1-11 125 Comparative Example 1-12 120 Comparative Example 1-13 138 Comparative Example 1-14 148 Control group 100

As shown in Table 3, it can be seen that the extracts of the present invention are superior to the control (untreated group) and the control group 1 in the proliferation rate of human dermal papilla cells, and thus the hair or hair loss prevention effect is more excellent.

<Experimental Example 2> Hair growth test>

In C57BL / 6 mice, hair growth and decay are comparable after 6-7 weeks of age, which is an effective model for hair growth experiments. The extracts of Examples 1-1 and 1-2, Comparative Example 1-1, Comparative Example 1-5 and Comparative Example 1-9 were selectively treated with this animal model to confirm the hair growth effect.

Six-week old female C57BL / 6 rats were adapted to the breeding environment for one week (12 hours / 12 hours at night, 24 hours, 40-50% humidity, free access to food and water) The epidermis was evenly depilated using an epilator. The extracts of Examples 1-1 and 1-2 and Comparative Example 1-1, Comparative Example 1-5 and Comparative Example 1-9 were lyophilized, and then prepared as a 100 μg / ml dilution. The extracts were applied twice daily to the rat epidermis , 1% (w / v) minoxidil diluent in the positive control group, and water only in the negative control group. After 30 days of observation, changes in the hair growth pattern were observed. The results are shown in Table 4.

Extract treatment conditions Preparation of Minoxidil Diluent
The amount of hair growth in the extract-treated group (%) Example 1-1 95 Examples 1-2 93 Comparative Example 1-1 35 Comparative Example 1-5 41 Comparative Example 1-9 45 Comparative Example 1-11 42 Positive control: 1% (w / v minoxidil diluent) 100 Negative control group 29

Referring to Table 4, it can be seen that when the extract of the herbal medicine mixture of the present invention is treated, excellent hair growth effect is obtained in all individuals (n = 5).

<Experimental Example 2: Prevention of hair loss and confirmation of wool effect by taking extract of crude drug mixture>

For each of the extracts of Example 1 and Comparative Example 1, patients who were in the dermatology department in their 30s to 50s visiting dermatologists from Sep. 2013, the extracts were divided into 5 groups each for 40 days, 110ml three times daily (5 points: excellent, 4 points) in the following Table 5 after taking and applying for 40 days. The results are shown in Table 5 below. Point: excellent, 3 points: normal, 2 points: poor, 1 point: very poor) to prevent hair loss and wool effect.

Condition Hair loss prevention and wool effect Example 1-1 3.9 Examples 1-2 4.1 Example 1-3 4.0 Examples 1-4 4.2 Examples 1-5 4.1 Comparative Example 1-1 2.4 Comparative Example 1-2 2.3 Comparative Example 1-3 2.8 Comparative Example 1-4 2.6 Comparative Example 1-5 2.9 Comparative Example 1-6 2.8 Comparative Example 1-7 2.7 Comparative Example 1-8 3.0 Comparative Example 1-9 3.1 Comparative Example 1-10 3.1 Comparative Example 1-11 2.9 Comparative Example 1-12 2.8 Comparative Example 1-13 3.0 Comparative Example 1-14 3.0 5 points: Excellent, 4 points: Excellent, 3 points: Normal, 2 points: Poor, 1 point: Very poor

Referring to Table 5, the extract of the crude drug mixture of the present invention shows excellent hair loss prevention and wool effect.

<Experimental Example 3> Toxicity test>

Experimental Example 3-1. Acute toxicity

To examine the toxicity of the herbal mixture (lyophilized product) obtained in Example 1-1 of the present invention to the animal body in an acute manner (within 24 hours) when an excessive amount of the herbal extract (lyophilisate) was consumed in a short period of time, Respectively. Twenty ICR mice were injected into each of the control and experimental groups. In the control group, only the PEG-400 / tween-80 / EtOH (8/1/1, v / v / v) -80 / EtOH (8/1/1, v / v / v) for oral administration. As a result, the mice were found to survive in the control group and in the experimental group to which the extract of the crude drug mixture of Example 1-1 of the present invention was administered at a concentration of 2 g / kg / day.

Experimental Example 3-2. Organ organs toxicity test in experimental group and control group

In the long-term toxicity test, the extract (lyophilisate) of the crude drug mixture of Example 1-1 of the present invention was administered to C57BL / 6J mice (10 rats per group) for 8 weeks at each concentration. In order to investigate the effect on the organs (tissues) of the animals, the experimental group to which the extract of the crude drug mixture of Example 1-1 of the present invention was administered and the PEG-400 / tween-80 / EtOH (8/1/1, v / 8 weeks after the administration of v / v alone, the blood was collected and the concentration of glutamate-pyruvate transferase (GPT) and blood urea nitrogen (BUN) in the blood was measured using a Select E (Vital Scientific NV, Respectively. As a result, GPT, which is known to be related to hepatotoxicity, and BUN, which is known to be related to renal toxicity, showed no significant difference compared to the control group. In addition, liver and kidney were cut from each animal, followed by a general tissue section preparation, histological observation with an optical microscope, and no abnormalities were observed in all tissues.

&Lt; Formulation Example 1: Preparation of external ointment for skin &

Skin external ointment was prepared according to a conventional method with the composition shown in Table 6 below.

Ingredients Addition amount (% by weight) The extract (lyophilized product) of the crude drug mixture of Example 1-1 1.0 S-nitrosoglutathione 0.04 Hyaluronic acid 3.0 lecithin 3.0 Diethyl sebacate 8.0 Nonsense 5.0 Polyoxyethylene oleyl ether phosphate 6.0 Paraoxybenzoic acid ester Suitable amount vaseline up to 100

&Lt; Formulation Example 2: Preparation of shampoo &

Shampoos were prepared according to the usual methods with the compositions shown in Table 7 below.

Ingredients Addition amount (% by weight) The extract (lyophilized product) of the crude drug mixture of Example 1-1 0.01 S-nitrosoglutathione 0.04 Hyaluronic acid 1.5 lecithin 3.0 30% sodium lauryl sulfate 20.0 30% sodium polyoxyethylene lauryl sulfate 20.0 Coconut oil fatty acid diethanolamide 2.00 Propylene glycol 2.00 Cocamidopropyl betaine 3.00 Carboxyvinyl polymer 0.30 Polyquaternium-10 0.50 Paraoxybenzoic acid ester 0.20 Citric acid Suitable amount Spices Suitable amount Purified water Balance

< Formulation Example  3. Manufacture of hair tonic>

doing A hair tonic was prepared by the usual method with the composition shown in Table 8.

Ingredients Addition amount (% by weight) The extract (lyophilized product) of the crude drug mixture of Example 1-1 0.1 S-nitrosoglutathione 0.04 Hyaluronic acid 1.5 lecithin 3.0 ethanol 50.00 L-menthol 0.10 D-Panthenol 0.20 glycerin 3.00 Salicylic acid 0.10 Topoceryl acetate 0.10 Polyoxyethylene hardened castor oil 0.50 Spices Suitable amount Purified water Balance

&Lt; Formulation Example 2: Food Preparation >

2-1. Manufacture of cooking seasonings

The lyophilized mixture extract (lyophilized product) of Example 1-1 of the present invention was added to the cooking seasoning at 1 wt% to prepare a cooking sauce for health promotion.

2-2. Manufacture of flour food products

(Lyophilized product) of the crude drug mixture of Example 1-1 of the present invention was added to flour at 0.1 wt%, and bread, cake, cookies, crackers and noodles were prepared using this mixture to prepare health promotion foods Respectively.

2-3. Manufacture of soups and gravies

The health enhancing soup and the juice were prepared by adding the extract (lyophilized product) of the crude drug mixture of Example 1-1 of the present invention to the soup and juice at 0.1 wt%.

2-4. Manufacture of dairy products

The lyophilized mixture extract (lyophilized product) of Example 1-1 of the present invention was added to milk in an amount of 0.1% by weight, and various dairy products such as butter and ice cream were prepared using the milk.

2-5. Vegetable juice manufacturing

0.5 g of the herbal mixture extract (lyophilized product) of Example 1-1 of the present invention was added to 1,000 ml of tomato juice or carrot juice to prepare vegetable juice for health promotion.

2-6. Manufacture of fruit juice

0.1 g of the herbal mixture extract (lyophilized product) of Example 1-1 of the present invention was added to 1,000 ml of apple juice or grape juice to prepare fruit juice for health promotion.

Claims (4)

Characterized in that it contains a water extract of a herbal mixture which is obtained by mixing 100 parts by weight of herringbone, 80 parts by weight of green tea, 80 parts by weight of green tea and 140 parts by weight of herbal tea at 70 to 100 ° C for 0.5 to 10 hours Composition. Characterized in that it contains a water extract of a herbal mixture which is obtained by mixing 100 parts by weight of herringbone, 80 parts by weight of green tea, 80 parts by weight of green tea and 140 parts by weight of herbal tea at 70 to 100 ° C for 0.5 to 10 hours Health functional foods. Characterized in that it contains a water extract of a herbal mixture which is obtained by mixing 100 parts by weight of herringbone, 80 parts by weight of green tea, 80 parts by weight of green tea and 140 parts by weight of herbal tea at 70 to 100 ° C for 0.5 to 10 hours Cosmetic composition. (Step 1): 100 parts by weight of Horseshoe cherry, 80 parts by weight of hemp leaf, 80 parts by weight of green tea, and 140 parts by weight of Hansikuchoshi, to prepare a herbal mixture;
(Step 2) Mixing the herbal medicine mixture of the above one step with water having a total weight of 1 to 10 times the total weight of the herbal medicine mixture and extracting the mixture at 70 to 100 ° C for 0.5 to 10 hours; And
(Step 3) removing the gut of the water extract of the crude drug mixture extracted in the above two steps and collecting the filtrate; &Lt; / RTI &gt;

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