KR102348042B1 - Composition for improving skin condition comprising extract of hydrangea serrata - Google Patents

Abstract

The present specification relates to a composition comprising an extract of cornflower hydrangea, and more particularly, to a composition exhibiting a skin moisturizing effect or atopic dermatitis improvement effect, acne improvement effect, and skin inflammation improvement effect by including a cornflower hydrangea extract. Therefore, the composition comprising the extract of cornflower hydrangea disclosed in the present specification can be usefully used as a pharmaceutical, food or cosmetic composition, and in particular can be used as an external preparation for skin to exhibit its effect.

Description

Composition for improving skin condition comprising extract of cornflower hydrangea {COMPOSITION FOR IMPROVING SKIN CONDITION COMPRISING EXTRACT OF HYDRANGEA SERRATA}

The present specification relates to a composition comprising an extract of cornflower hydrangea.

The most important function of the epidermis, which is located in the outermost part of the skin, is to defend against various external stimuli (chemicals, air pollutants, dry environment, physical and chemical irritants such as UV rays) and to retain moisture in the body through the skin. It is a protective function to prevent excessive divergence, and this protective function can only be maintained when the stratum corneum composed of keratinocytes is normally formed. The stratum corneum (horney layer), which is the outermost layer of the epidermis, is formed from keratinocytes, and consists of keratinocytes that have completed differentiation and a lipid layer surrounding them (J. Invest. Dermatol. 1983;80:44~). 49). Keratinocytes are characteristic cells in which basal cells, which continuously proliferate in the lowermost layer of the epidermis, undergo morphological and functional changes in stages, and rise to the surface of the skin. It is eliminated and new keratinocytes take over their functions. This repetitive series of changes is called 'epithelial cell differentiation' or 'keratinization'. During the keratinization process, keratinocytes form the stratum corneum while producing natural moisturizing factor (NMF) and intercellular lipids (ceramide, cholesterol, fatty acid), which makes the stratum corneum firm and flexible, thereby providing a skin barrier. ) to retain its function.

However, the stratum corneum can easily lose its function due to excessive washing, lifestyle factors such as bathing, environmental factors such as dry air, pollutants, and intrinsic diseases such as atopic skin or senile skin, etc. Due to various factors that have increased in modern times, the number of people who are complaining of dry skin symptoms and various disorders due to them is increasing. Therefore, many studies have been conducted to supply moisture from the outside in order to maintain proper skin moisture or to prevent moisture loss from the body. It is widely used in the field.

However, the formation-fallover rate of the stratum corneum is slowed, the lipid synthesis ability of keratinocytes is reduced, or normal cell division, maturation and differentiation in the epidermis is not smooth. There is an increasing trend in cases in which the amount is reduced and the skin has a state in which the function of the normal stratum corneum cannot be maintained, that is, in a state in which the skin barrier function is disrupted.

Atopic dermatitis, which appears due to the breakdown of the skin barrier function, is a chronic, recurrent skin disease that often accompanies itching. Although the etiology of atopic dermatitis is not yet clear, it is known that it is caused by an immunological abnormality mediated by type 2 T helper lymphocytes, and is caused by environmental allergens, genetic predisposition, psychological factors, or pharmacological factors.

Psoriasis is a chronic inflammatory dermatosis in which red papules or plate-like rashes of various sizes occur repeatedly on the skin of the body. It is believed to stimulate keratinocytes, causing excessive proliferation and inflammation.

Recently, as it was revealed that an abnormally activated immune response, such as hypersensitivity due to hyperactivity of T lymphocytes and hypersecretion of IgE, is one of the main causes of atopic dermatitis, contact dermatitis, and psoriasis disease, excessive immunity Approaches to treat these skin diseases by inhibiting the response are of interest.

Due to the division and differentiation of these abnormal epidermal cells, the skin causes various skin diseases such as dry skin, atopic dermatitis and psoriasis. However, it is difficult to expect a fundamental cure.

Acne, which is one of the representative skin inflammatory diseases, refers to an inflammatory disease of the hair follicles, hair follicles, and surrounding tissues caused by the action of hormones, excessive sebum secretion, and bacterial infection. There are many causes of acne, but the main causes are excessive secretion of sebum and infection of pores due to excessive secretion of sebum. On the surface of the skin where the pH is increased due to excessive sebum, acne-causing bacteria easily proliferate, and the proliferated acne-causing bacteria causes purulent inflammation through weakened skin.

Acne treatment includes topical application therapy, oral drug administration, and surgical therapy, such as laser and dermabrasion, which are currently being performed. Topical application therapy uses antibacterial agents such as azelaic acid and salicylic acid for the purpose of antibacterial action against Propionibacterium acnes, which is known as the causative agent of acne, or benzoyl peroxide (benzoyl peroxide) for the purpose of dissolving comedones. peroxide) as an external ointment type. However, it is difficult to commercialize it due to formulation and cosmetic raw material laws. Oral drug administration uses retinoids and corticosteroids for the purpose of suppressing the function of the sebaceous glands and the destruction of the hair follicle wall. Therefore, caution is required when using it.

Recently, in order to solve the above problems while reducing the side effects of the skin caused by various chemicals, etc., a lot of compositions for external application for skin such as cosmetics using natural products have been developed. Accordingly, the present inventors have studied the possibility of application as an external preparation for skin from plants that are not widely known so far.

KR 2002-0070566 A

J. Invest. Dermatol. 1983;80:44~49

The inventors of the present specification have completed the present invention by confirming that the corn hydrangea extract is safe for the skin and can enhance the excellent moisturizing effect and skin immunity through repeated and various experiments.

Accordingly, an object of the present specification is to provide a composition for external application to the skin comprising an extract of cornflower hydrangea.

In order to achieve the above object, the present specification provides a composition for external application for skin for improving skin immunity and moisturizing the skin, comprising an extract of cornflower hydrangea as an active ingredient.

The composition of the present specification exhibits a skin moisturizing effect and an immunity enhancing effect by including the extract of cornflower hydrangea, and through this, it can be used in various ways in the cosmetic or pharmaceutical field.

In one aspect, the present specification may relate to a composition comprising an extract of hydrangea ( Hydrangea serrata ) as an active ingredient.

In one aspect of the present specification, the composition may be a pharmaceutical, food, or cosmetic composition.

In one aspect of the present specification, the composition may be a composition for improving skin condition.

In one aspect of the present specification, skin condition improvement may be skin moisturizing, skin barrier function enhancement, anti-inflammatory, antibacterial, sebum secretion suppression or acne improvement, prevention or treatment.

In one aspect of the present specification, the composition may be a composition for improving, preventing or treating atopic dermatitis, xeroderma, or psoriasis.

In one aspect of the present specification, the antibacterial may be an antibacterial composition against acne bacteria. The acne bacteria may be propionibacterium acnes.

In one aspect of the present specification, the hydrangea may be one or more selected from the group consisting of whole plants, leaves, stems, branches, bark, flowers, seeds and fruits of the woody hydrangea.

In one aspect of the present specification, the cornflower extract may be extracted by mixing leaves, fruits and bark in a weight ratio of 1 to 10:1 to 3:1. The weight ratio may be a weight ratio of 6-8: 1.5-2.5: 1 or a weight ratio of 7:2:1.

In one aspect of the present specification, the cornflower extract may be one or more extracts selected from the group consisting of water, an organic solvent, and a mixture thereof. The organic solvent may be at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol.

In one aspect of the present specification, the composition may be a composition for external application to the skin.

And arithmetic station (Hydrangea serrata) is in hydrangea plants (Hydrangea) herein, the genus Hydrangea macrophylla, Hydrangea and belongs to the (Hydrangeaceae), consists of a neck nature shrub of about 23 species. It is native to the Western Hemisphere and Eastern Asia. Hydrangea plants grown in Korea include hydrangea ( H. serrata ) and hydrangea. Hydrangeas and hydrangeas grow in mountains or fields, and the varieties planted in the garden are hydrangeas and their varieties. Although hydrangea can be found all over Korea, water hydrangea only grows on Ulleungdo and Jeju Island. In addition, tree hydrangeas and American hydrangeas are imported from abroad and planted for landscaping in gardens.

Hydrangea serrata referred to herein is, Hydrangea Serrata for Acuminata (S. et z.) Wilson (Hydrangea Serrata for . acuminata (Siebold & Zucc) Wilson), Hydrangea serrata for Hydrangea serrata for. buergeri Nakai., Hydrangea serrata for Koreana tea. Lee ( Hydrangea serrata for. coreana T. Lee) and Hydrangea serrata for. fertilis Nakai.

In the present specification, hydrangea Serrata for acuminata (S. et z.) Wilson (Hydrangea Serrata for. acuminata (Siebold & Zucc) Wilson), hydrangea (Lee Chang-bok, Korean botanical encyclopedia, 1984) or flower landscape It is also called soup (Songhoop, 1980, Korea Plant Resources), and is also called hairy hydrangea. It is a dicotyledonous broad-leaved shrub of the Rosaceae family of the Rosaceae family. It grows wild at an altitude of 200-1,400m in the south central part of Korea, and is also distributed in Japan and Taiwan. It usually grows up to 1m, grows by giving out many stems under the stem, and there are fine hairs on annual branches. The root has a fine root and a normal root, and the leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, and the lower part is round or acute, 5~15cm long and 2~10cm wide, with sharp saw teeth on the edge. There are hairs on the side veins and the back veins. The flowers are in July-August, with large flat inflorescences hanging at the tip of the branch grown in the current year, hairy, and the non-sexual flowers around them are 2-3cm in diameter, and the sepals are like petals and have 3-5 pieces, white, reddish-walled or wall-colored. Bisexual flowers have small sepals and 5 each together with petals. There are 5 stamens, and the length is 3~4mm together with the style, and there is 1 pistil and 3~4 styles. The fruits ripen in September-October and are small, large honey egg-shaped capsules.

In the present specification, Hydrangea serrata for. buergeri Nakai. is also called flower landscape hydrangea (Lee Chang-bok, Korean Botanical Encyclopedia, 1984) or Goryeo hydrangea (Songjiok, 1980, Korea Plant Resources). . It is distributed in the south central part of Korea including Jeju Island, Japan, and Taiwan. The growing temperature is 16~30℃, and it grows well in the waterside or valleys with a lot of soil moisture. Grows up to 1 m in height. The leaves are opposite, long oval, and pointed at the tip. The length of the leaf is 5~15cm and the width is 2~10cm, with saw teeth on the edge and hairs on the veins. The flowers are white or blue-purple, and hang in coaxial inflorescences at the end of branches in July-August. Nearby neuter flowers have 3 to 5 sepals, serrated, and look like petals. A bisexual flower hangs in the center, and there are 5 petals and 5 sepals each. The fruit is a capsule (朔果) which ripens in October and has fine roots.

In the present specification, hydrange Serrata for Koreana tea. Lee ( Hydrangea serrata for. coreana T. Lee), also called cotyledonous hydrangea (Lee Chang-bok, Korean Botanical Encyclopedia, 1984) or long-leaved hydrangea (Songjiok, Korea Plant Resources, 1980), is a deciduous broad-leaved shrub of the dicotyledonous family Rosaceae. . Distributed in central and south of Korea, it usually grows up to 1m, grows by giving out many stems under the stem, and there are fine hairs on annual branches. The root has a fine root and a normal root, and the leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, and the lower part is round or acute, 5~15cm long and 2~10cm wide, with sharp saw teeth on the edge. There are hairs on the side veins and the back veins, and the leaves are especially thick. Flowering period is July-August, and numerous bisexual flowers form flat inflorescences together with decorative flowers at the ends of branches grown in the current year. In decorative flowers, sepals are in the shape of petals, 3 to 5 pieces, and are blue or white. Bisexual flowers have small sepals and 5 each together with petals. Stamens are 10, longer than petals. There is 1 pistil and 3 to 4 styles. The fruits ripen in autumn and are egg-shaped capsules.

In the present specification, hydrangea serrata for fertilis Nakai (Hydrangea serrata for. fertilis Nakai.) is called Tamna hydrangea (Songjiok, Korea Plant Resources, 1980) or island hydrangea, and is a deciduous shrub of the dicotyledonous plant Rosaceae. . It is distributed in Jeju Island, Korea, and grows at the foot of a mountain or at the edge of a forest. It grows up to about 1m in height, grows by producing many stems from the bottom, and has fine hairs on annual branches. The leaves are opposite, oval or egg-shaped, with a long pointed tip like a tail, and the lower part is round or acute, 5-15 cm long and 2-10 cm wide, with sharp saw teeth, and hairs on the side veins on the surface and on the veins on the back side. . Flowers bloom in July-August, and large flat inflorescences hang at the tip of the branch grown in the current year, and there are hairs. Numerous bisexual flowers form large flat inflorescences along with nonsexual flowers with a diameter of 2-3 cm. Asexual flowers have sepals in the shape of petals, 3 to 5, and are blue or purple. Bisexual flowers have small sepals and 5 each together with petals. Stamens are 10, longer than petals, and pistil is 1, and style is 3-4. The fruits are egg-shaped and ripen in autumn.

The hydrangea used in this specification was sampled from plants planted as ornamental trees at the Osulloc Tea Museum in Seogwang Tea Garden, Jeju.

The method for preparing the cornflower extract used in the present specification is not particularly limited, and in the present specification, the cornflower hydrangea extract is a leachate obtained by leaching and discharging from a cornflower hydrangea, a concentrate obtained by concentrating part or all of the leachate again, and the concentration again Contains extracts prepared by drying water, or chemical substances that exert the main effect contained in extracts.

Cornflower hydrangea as used herein may be included in the form of an extract, a pulverized product of the crude drug itself, or a dry pulverized product of the crude drug, but is not limited thereto. In addition, the hydrangea used in this specification is not limited in its acquisition method, it can be cultivated and used or purchased and used on the market, and the whole wood, part or all of the above-ground part or the root part can be used, preferably the above-ground part can use all of Specifically, at least one selected from the group consisting of leaves, flowers, branches, stems, roots, bark, seeds and fruits of the woody hydrangea may be used.

In the present specification, "extract" includes all substances obtained by extracting the components of natural products, regardless of the extraction method, extraction solvent, extracted components, or the form of extracts It is a broad concept that includes all substances obtainable by processing or processing by a method.

In the present specification, "corn hydrangea extract" includes all substances obtained by extracting the components of cornflower hydrangea, regardless of the extraction method, extraction solvent, extracted component or the form of the extract, and in the process of extracting the component, heat, acid (acid), base (base), includes a substance obtained through an extraction method that includes a process with enzymes, etc., and can also be obtained by processing or treating the material obtained by extracting the components of cornflower hydrangea by other methods after extraction It is a broad concept that includes all substances that exist.

In one aspect of the present specification, the cornflower extract comprises the steps of washing and drying cornflower; solvent extraction of dried cornflower; And it can be obtained through the step of filtering the extracted cornflower. In the above, the solvent includes at least one selected from the group consisting of water, an organic solvent, and a mixture of water and an organic solvent. In the above, water includes distilled water or purified water, and the organic solvent is selected from the group consisting of alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, and chloroform, for example, _{C 1} to C _{5 lower alcohol.} One or more include, but are not limited to.

The cornflower extract may include a cornflower-C ₁ ~C ₆ alcohol extract, and specifically, the alcohol may be methanol or ethanol.

In the present specification, the cornflower extract may be a crude extract of a solvent selected from the group consisting _{of water, C 1} -C _{6 alcohol, and a combination thereof.} The C ₁ -C ₆ alcohol may be specifically methanol. When extracting cornflower hydrangea with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times that of cornflower hydrangea, and specifically, it is preferable to extract by adding about 10 times the solvent, but is not limited thereto. For the extraction, heating extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, etc. may be used, and there is no limitation as long as the extraction method is obvious to those skilled in the art. The extraction may be carried out at room temperature, but for more efficient extraction, it may be performed under heating conditions, preferably at a temperature of about 40 to 100°C, more preferably at a temperature of about 80°C, but limited thereto it is not The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but is not limited thereto, and may vary depending on conditions such as extraction solvent and extraction temperature. The extraction may be performed one or more times in order to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times consecutive extraction, and the combined extract may be used.

In the present specification, the extract of cornflower hydrangea may include a crude extract of cornflower hydrangea as described above, and may also be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having low polarity. The organic solvent may be hexane, methylene chloride, ethyl acetate, n-butanol, or the like, but is not limited thereto. The extract extracted by the above method or a soluble fraction of the extract may be used as it is, but it may be used in the form of an extract by concentration after filtration, or in the form of a freeze-dried product after concentration and freeze-drying.

As used herein, the term “skin” refers to a tissue covering the body surface of an animal, and is a broad concept including not only tissues covering body surfaces such as the face or body, but also the scalp.

Corneous hydrangea extract of the present specification is cut and dried; Adding water, an organic solvent, or a combination thereof to the dried cornflower hydrangea and extracting under reflux; After depositing the extract, filtering with a filter cloth; centrifuging the filtrate to separate the residue and the filtrate; It can be obtained by a method comprising; concentrating the separated filtrate under reduced pressure to obtain an extract of cornflower hydrangea.

In one aspect of the present specification, the cornflower extract is composed of purified water, ethanol, butylene glycol, and propylene glycol so that the cornflower hydrangea concentrate concentrated under reduced pressure according to the above method is included in 5% by weight based on the total weight of the extract. It may be prepared by dispersing/dissolving using one or more solvents selected from the group.

In addition, in the extraction process of the corn hydrangea extract of the present specification, for example, water or an organic solvent is added to the chopped and dried corn hydrangea, extracted under reflux and deposited, and then the residue and the filtrate are separated through filter cloth filtration and centrifugation, The separated filtrate can be concentrated under reduced pressure to obtain a cornflower extract. The organic solvent usable herein may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform, or a mixed solvent of these organic solvents and water, and preferably 100% water, 70% ethanol in consideration of the safety of raw materials Or butylene glycol (Butylene Glycol) is used. At this time, the extraction temperature is preferably 18 ~ 80 ℃ in the case of water, 18 ~ 40 ℃ in the case of 70% ethanol and butylene glycol, and the extraction time is preferably 6 ~ 168 hours. If the extraction temperature and extraction time are out of the range, extraction efficiency may decrease or a change in components may occur. After obtaining the extract using the solvent in the above, the liquid may be obtained by cooling, heating, and filtration at room temperature in a conventional method known in the art, or the solvent may be further evaporated, spray-dried or freeze-dried. In addition, during freeze-drying, freeze-drying may be performed at -50 to -70°C for 3 to 4 days.

The composition of the present specification preferably includes the cornflower hydrangea extract in an amount of 0.001 to 50% by weight based on the total weight of the composition. More preferably, 0.1 to 1% by weight or 0.5 to 10% by weight may be used. In the case of less than 0.001% by weight, it is difficult to expect the efficacy, and when 50% by weight is included, a problem in formulation occurs. Specifically, the cornflower extract is 0.001% by weight or more, 0.01% by weight or more, 0.1% by weight or more, 0.3% by weight or more, 0.5% by weight or more, 0.8% by weight or more, 1% by weight or more, 3% by weight or more with respect to the total weight of the composition. , 5 wt% or more, 7 wt% or more, 10 wt% or more, 15 wt% or more, 20 wt% or more, 25 wt% or more, 30 wt% or more, 35 wt% or more, or 40 wt% or more, and 60 wt% or more % or less or 50 wt% or less.

In one aspect of the present specification, the concentration of the cornflower extract may be 0.01ug/mL to 100ug/mL. In addition, the concentration of the cornflower extract is 0.01ug/mL or more, 0.1ug/mL or more, 0.3ug/mL or more, 0.4ug/mL or more, 0.5ug/mL or more, 0.6ug/mL or more, 0.7ug/mL or more, It may be 1ug/mL or more, 5ug/mL or more, 10ug/mL or more, 100ug/mL or more, or 1000ug/mL or more, and may be 100mg/mL or less or 10mg/mL or less.

The pharmaceutical composition according to the present specification may be in various oral or parenteral formulations. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, etc., and such solid preparations include at least one excipient in one or more compounds, for example, starch, calcium carbonate, sucrose Alternatively, it is prepared by mixing lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, internal solutions, emulsions, syrups, etc. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

The pharmaceutical dosage form of the composition of the present specification may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds as well as in an appropriate group. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, and the like can be used.

The composition of the present specification may be administered parenterally or orally as desired, and administered in an amount of 0.1 to 500 mg, preferably 1 to 100 mg per 1 kg of body weight per day, divided into 1 to several times administered. have. The dosage for a specific patient may vary depending on the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, severity of disease, and the like.

The pharmaceutical composition according to the present specification is prepared according to a conventional method according to a conventional method, respectively, in oral dosage forms such as powders, granules, tablets, soft or hard capsules, suspensions, emulsions, syrups, aerosols, ointments, creams, etc. And it can be formulated and used in any form suitable for pharmaceutical preparations, including sterile injection solutions.

The composition according to the present specification may be administered to mammals such as mice, mice, livestock, and humans by various routes such as parenteral and oral administration, and all modes of administration can be expected, for example, oral, rectal Or it may be administered by intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

In one aspect of the present specification, the food composition may be a health functional food composition.

The formulation of the food composition according to the present specification is not particularly limited, but may be formulated as, for example, tablets, granules, powders, liquids such as drinks, caramel, gels, bars, and the like. In addition to the active ingredient, the food composition of each dosage form can be appropriately selected by those skilled in the art without difficulty depending on the dosage form or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

In the food composition according to the present specification, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage thereof is, for example, 0.1 mg/kg/day to 5000 mg/kg/day, more specifically 50 It may be from mg/kg/day to 500 mg/kg/day, but is not limited thereto, and may vary depending on various factors such as age, health status, and complications of the subject to be administered.

The food composition according to the present specification, for example, various foods such as chewing gum, caramel products, candies, frozen desserts, and confectionery, soft drinks, mineral water, beverage products such as alcoholic beverages, vitamins and minerals, etc. Health functional foods can

In addition to the above, the food composition of one aspect of the present specification includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the functional food compositions of the present specification may include natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition herein.

The cosmetic composition according to one aspect of the present specification is not particularly limited in formulation, and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing It may be prepared in any one or more formulations selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser, but is not limited thereto.

When the formulation of the cosmetic composition according to the present specification is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

When the formulation of the cosmetic composition according to the present specification is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluoro propellants such as hydrocarbons, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition according to the present specification is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition according to the present specification is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

In the case of surfactant-containing cleansing of the cosmetic composition according to the present specification, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The cosmetic composition according to the present specification may further include functional additives and components included in general cosmetic compositions in addition to the extract of cornflower hydrangea. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

In the cosmetic composition of this specification, you may mix|blend the component contained in a general cosmetic composition as needed with the said functional additive further. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

Furthermore, the present specification relates to an external preparation for skin containing an extract of cornflower hydrangea as an active ingredient, and the external preparation for skin is a generic term that may include anything applied outside the skin, and cosmetics of various formulations may be included here.

Hereinafter, the configuration and effect of the present specification will be described in more detail with reference to Examples, Test Examples and Preparation Examples. However, these Examples, Test Examples, and Preparation Examples are provided only for the purpose of illustration to help the understanding of the present specification, and the scope and scope of the present specification are not limited by the following examples.

[Preparation Example 1] Preparation of hydranase Serrata for acuminata (S. et z.) Wilson extract

The leaves, fruits, and bark of Hydrangea Serrata for . acuminata (Siebold & Zucc) Wilson, chopped and dried in the shade, were mixed in a weight ratio of 7:2:1. 1 kg was extracted under reflux 3 times for 24 hours with 70% ethanol aqueous solution, cooled, and filtered with Whatman #5 filter paper. The filtered extract was concentrated under reduced pressure at 45°C or lower and freeze-dried at -60°C for 3 days.

The extract was prepared by dispersing/dissolving the concentrate under reduced pressure using ethanol so that it was included in 5% by weight based on the total weight of the extract of cornflower hydrangea.

[Preparation Example 2] Preparation of hydranase Serrata for Bujeri Nakai extract

An extract of Hydrangea serrata for. buergeri Nakai. was prepared in the same manner as in Preparation Example 1.

[Preparation Example 3] Hydrant Serrata for Koreana Tea. Preparation of Li extract

Hydrant Serrata for Koreana Tea in the same manner as in Preparation Example 1. An extract of Hydrangea serrata for. coreana T. Lee was prepared.

[Preparation Example 4] Preparation of hydranase Serrata p. pertilis Nakai extract

An extract of Hydrangea serrata for. fertilis Nakai. was prepared in the same manner as in Preparation Example 1.

[Examples 1 to 4, Comparative Example 1]

In order to use the composition of the present specification, a composition containing the cornflower extract of Preparation Examples 1 to 4 was prepared as a cream according to the composition specified in Table 1 below (Hydrant Serrata for Acuminata (S. et z). .) Wilson (Example 1), Hydrant Serrata for Bujeri Nakai (Example 2), Hydra Serrata for Koreana T. Lee (Example 3), Hydrant Serrata for Bujeri Nakai (Example 3) Nakai (Example 4)). For comparison, a cream formulation without cornflower extract was used as a comparative example.

ingredient Examples 1 to 4 (wt%) Comparative Example 1 (wt%) Purified water to 100 to 100 Any one cornflower extract of Preparation Examples 1 to 4 0.5 - vegetable hydrogenated oil 1.5 1.5 stearic acid 0.6 0.6 stearyl alcohol 2.0 2.0 glycerol stearate 1.0 1.0 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.0 1.0 Arachidyl Behenyl Alcohol & Arachidyl Glucoside 1.0 1.0 Cetylaryl Alcohol & Cetearyl Glucoside 2.0 2.0 PEG-100 stearate & glycerol oleate & propylene glycol 1.5 1.5 Caprylic/Capric Triglycerides 11 11 Cyclomedicone 6 6 triethanolamine 0.1 0.1

[Comparative Example 2]

In Preparation Examples 1 to 4, a willow extract was obtained through the same method by using cornflower as a willow tree.

[Test Example 1] Skin moisturizing effect test

In order to find out how long it lasts for skin moisture when applied once, the cosmetic compositions of Examples 1 to 4 and Comparative Example 1 were administered to 50 adults in their 30s and 40s who complained of dryness or who had dry skin. After allowing it to be applied to the skin, the moisture content of the skin was measured using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) for 24 hours. The coniometer measures the amount of moisture present in the epidermis of the skin by using a sensor to measure the ion level of moisture and quantify it to calculate the amount of moisture, and the measurement method is as follows.

1) Place the coniometer probe on the skin area to be measured.

2) When the probe is pressed on the skin, the capacitance of the skin is digitized through the sensor and displayed on the screen.

3) The measurement was repeated by changing the measurement site.

4) After measuring once, wipe the sensor with a tissue such as Kimwipe and measure again.

In the above method, the moisture content of the skin was measured using a coniometer under constant temperature and humidity conditions (24° C., humidity 40%) before the start of application in the above method and used as a basic value, and the change after 12 hours and 24 hours was measured. The results are shown in Table 2 below.

sample Skin moisture (%) the battlefield after 12 hours after 24 hours Example 1 24.1 37.2 34.0 Example 2 23.9 37.1 33.7 Example 3 24.2 37.5 34.1 Example 4 24.5 37.9 34.3 Comparative Example 1 25.4 33.1 26.0

From the results of Table 2, it can be seen that when Comparative Example 1 was applied, the skin moisture content increased to some extent after 12 hours after application, but returned to the state before application after 24 hours. On the other hand, when Examples 1 to 4, which are compositions for external application for skin containing a cornflower extract, are applied to the skin, it can be seen that the moisturizing power lasts for 24 hours or more, and the effect of increasing the skin moisture content is also higher than that of Comparative Example 1.

Therefore, it can be confirmed that the composition for external application for skin including the extract of cornflower hydrangea according to one aspect of the present specification has a remarkable skin moisturizing effect.

[Test Example 2] Evaluation of skin dryness (or psoriasis) improvement effect

At the time of completion of Test Example 1, after using Examples 1 to 4 and Comparative Example 1 for test subjects, subjective efficacy evaluation was conducted through a questionnaire on the improvement effect of dry skin. In the survey, satisfaction was divided into very good, good, average and poor for the degree of skin dryness improvement effect, and the results are shown in Table 3 below.

number of sample respondents very good Good usually Inadequate Example 1 8 29 12 One Example 2 9 28 12 One Example 3 9 29 11 One Example 4 10 29 9 2 Comparative Example 1 5 19 18 7

As can be seen from the results of Table 3, in Comparative Example 1, the number of persons marked as good or higher was 24, whereas Examples 1 to 4, which are compositions for external application for skin including the cornflower hydrangea extract according to the present specification, were 37 persons, respectively, 37, 38, and 39 were significantly increased compared to Comparative Example 1. Therefore, the cases of Examples 1 to 4, which are compositions for external application for skin including the extract of cornflower hydrangea according to the present specification, were more severe than those of Comparative Example 1 (or psoriasis). ), it can be seen that the improvement, prevention, or treatment effect is excellent.

[Test Example 3] Evaluation of the improvement effect of atopic dermatitis

Subjective efficacy evaluation was performed through a questionnaire on the improvement effect of dry skin after using Examples 1 to 4 for patients with acute exacerbated atopic dermatitis. The use of Examples 1 to 4 was applied once in the morning and then once in the evening according to the user's life pattern. At the time of application, the cream was applied directly to the skin as if using a cosmetic cream, and the cream was applied to the area where symptoms of atopic dermatitis appeared. One and the same examiner evaluated the degree of improvement of the patient's skin lesion and the SCORing Atopic Dermatitis (SCORAD) score, and the patient recorded a visual analogue scale (VAS, 0~10 cm) to evaluate the severity of itching. did The evaluation and recording were made within 1 hour after application. After the experiment, the results are shown in Table 4 below.

the battlefield After 1 application After 2 coats SCORAD score Example 1 53.10 41.80 36.50 Example 2 53.10 40.20 34.30 Example 3 53.10 40.12 33.90 Example 4 53.10 37.50 34.00 VAS score Example 1 9 6 4 Example 2 9 6 4 Example 3 9 5 3 Example 4 9 5 2

In the case of a SCORAD score of 40 or higher, atopic symptoms are considered serious. As can be seen from the above results, when the external composition of Examples 1 to 4 was applied, the scores from 53.10 were all decreased to 40 or less, confirming that the atopic condition was improved.

Also, in the case of the VAS score, the closer to 10, the more severe the pain. It can be judged that the pain felt by the patient due to atopic dermatitis is significantly reduced.

Therefore, due to repeated application of Examples 1 to 4, both the SCORAD score and the VAS score decreased, so that the overall atopic dermatitis symptoms including inflammatory reaction and itching in the body were improved, prevented and treated, and the skin moisturizing effect was exhibited. can be known

[Test Example 4] Antibacterial activity test against acne bacteria

Antibacterial activity was tested with the compositions of Examples 1 to 4 and Comparative Example 1 against Propionibacterium acnes <ATCC 6919: Medium-BHI broth>, which is an acne causative strain.

The antibacterial activity test method for acne bacteria was as follows.

(1) Preparation of test bacteria

Propionibacterium acnes was inoculated into BHI broth and anaerobic culture was used.

(2) Preparation of dilution solution

0.15 ml of the test bacterial solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well, which was used as a dilution solution.

(3) Sample preparation

Examples 1 to 4 and the cream, which is the cosmetic composition prepared in Comparative Example 1, was used as a sample as it was.

(4) Antibacterial power test

1) Put a sample in row 1 of a 96-well cell culture tube (96-well plate) to match the starting concentration of 0.4%, and adjust the total amount to 200ul with a dilution solution. Add 100ul each of the dilution solution to the remaining wells.

2) Mix well the mixture from row 1, take 100 ul, put it in row 2, mix well, then take 100 ul again and put it in row 3 to do double dilution.

3) After stationary culture at 32°C for 24 hours and 48 hours, the presence or absence of proliferation of bacteria is determined to the extent of suspension, and the minimum concentration without proliferation of bacteria is determined as the MIC (Minimum Inhibitory Concentration) value. If the mixture is opaque and it is difficult to determine whether or not the growth of bacteria is present, check through microscopic observation.

The results of the antibacterial activity test against Acne bacteria are shown in Table 5 below.

sample pH Propionibacterium acnes Example Example 1 5.37 <5ppm Example 2 5.32 <4.9ppm Example 3 5.33 <5ppm Example 4 5.29 <4.8ppm Comparative Example 1 5.63 <10ppm

Examples 1 to 4 had antimicrobial activity of MIC 5, 4.9, 5, and 4.8ppm, respectively, against the test bacteria. As the ppm concentration in the MIC is lower, it can be said that it is an effective substance against acne bacteria at a lower concentration, so it can be judged that the antibacterial effect of acne bacteria is more excellent. Therefore, it was confirmed that Examples 1 to 4 exhibited a remarkable effect of about 2 times higher in antibacterial activity against Acne acnes because the MIC concentration for inhibiting Propionibacterium acnes was about 2 times lower than that of Comparative Example 1. Therefore, it can be confirmed that the composition comprising the extract of cornflower hydrangea according to the present specification has excellent antibacterial effect against acne bacteria.

[Test Example 5] Acne improvement, sebum secretion reduction and stimulation test

Twelve people with acne were allowed to use the cream, which is the cosmetic composition prepared in Examples 1 to 4 and Comparative Example 1, for one month. Acne improvement scale was scored from 1 to 5, with 1 being ‘not’, 3 points being ‘moderate’, and 5 points being ‘very much’. The experimental results are shown in Table 6 below as the average score of 12 participants.

Acne disappearance time was based on the number of days that the disappearance was read, and acne recurrence was based on the results after 1 month. Decreased sebum secretion was scored on a scale of 1 to 5, with 1 being ‘not’, 3 points being ‘moderate’, and 5 points being ‘very much’. The experimental results are shown in Table 6 below as the average score of 12 participants. The presence or absence of skin irritation was evaluated as (number of people who showed an irritant response)/(total number of test subjects).

Improves Inflammatory Acne When comedonal acne disappears acne recurrence reduced sebum secretion with or without stimulation Example Example 1 4.37 4 days radish 4.03 1/12 Example 2 4.5 4 days radish 4.05 1/12 Example 3 4.44 3 days radish 4.09 2/12 Example 4 4.62 3 days radish 4.11 1/12 Comparative Example 1 3.1 8 days you 2.97 3/12

As shown in Table 6, in Examples 1 to 4, compared to Comparative Example 1, acne did not recur, and an average of 4 or more points for improvement of inflammatory acne, an average of 4 days or less for the disappearance time of comedonal acne, and a decrease in sebum secretion By obtaining an average of 4 or more points, it can be seen that there is an excellent effect on acne improvement and preventive treatment. In addition, it can be seen that there is an excellent effect on the suppression of sebum secretion.

Therefore, it can be seen that the corn hydrangea extract is suitable for patients with acne skin when looking at various factors such as stimulation, improvement rate, improvement degree, and reduction of sebum secretion.

[Test Example 6] Inflammation improvement effect

The anti-inflammatory effect was evaluated by the effect of inhibiting the production of prostaglandins. The effect was measured on macrophages (purchased from ATCC) using the previously extracted extract. First, the cyclooxygenase (“COX”) activity remaining in the cells was irreversibly inhibited by adding aspirin to the macrophages collected from the abdominal cavity of the mouse so that the final concentration was 500M. Then, 100 μl of the electric suspension was put into each well of a 96-well cell culture tube and cultured for 2 hours in an incubator under 5% CO2 and 37° C. to attach macrophages to the surface of the vessel. Then, the adherent macrophages were washed 3 times with PBS and then used for the effect test of the extract. After culturing for 12 hours by adding RPMI (Roswell Park Memorial Institute medium) medium containing 1% (w/v) LPS to 5x104 cells (cell)/ml of electrocultured macrophages, the production of prostaglandins was induced and prepared 100 μl of the corn hydrangea extract of Examples 1 to 4 and the willow extract of Comparative Example 2 were treated at a concentration of 0.5 ug/mL, and prostaglandins released were quantified using an enzyme immunoassay (ELISA). At this time, the prostaglandin production inhibitory activity of the extract was determined by setting the difference between the prostaglandins generated in the LPS-treated group and the non-treated group to 100%, and the percentage of prostaglandins reduced by treating the LPS and the sample together with the control group and the control group. The comparison and determination were made, and the results (the effect of inhibiting the production of prostaglandins) are shown in Table 7 below.

Blank 100% Control group (aspirin treatment group) 25% production example Preparation Example 1 35.5% Preparation 2 37.8% Preparation 3 38.0% Preparation 4 37.9% Comparative Example 2 21.5%

As shown in Table 7, it was found that the inhibitory effect on the production of prostaglandins according to Preparation Examples 1 to 4 was very high compared to the control group treated with aspirin. In addition, in the case of Preparation Examples 1 to 4, which is an extract of cornflower hydrangea according to the present specification, it was found that the inhibitory effect of prostaglandin production was very high even when compared with Comparative Example 2, which is a willow extract. Therefore, it was confirmed that the cornflower extract according to the present specification exhibits an excellent anti-inflammatory effect.

Formulation examples of the composition according to one aspect of the present specification will be described below, but other various formulations are also applicable, which is not intended to limit the present specification, but merely to describe it in detail.

[Formulation Example 1] Soft capsule

Mixing cornflower extract of any one of Preparation Examples 1 to 4, 8 mg, vitamin E 9 mg, vitamin C 9 mg, palm oil 2 mg, hydrogenated vegetable oil 8 mg, yellow wax 4 mg, and lecithin 9 mg, and mixing according to a conventional method to obtain a soft capsule filling solution manufacture Soft capsules are prepared by filling 400 mg per capsule. Separately, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol solution, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present specification.

[Formulation Example 2] Tablet

After mixing 8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose, granulation using a fluidized bed dryer, sugar ester (sugar ester) 6 mg is added. 500 mg of these compositions are compressed in a conventional manner to prepare tablets.

[Formulation Example 3] Drink agent

After mixing 8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharide, 300 ml of purified water is added, and 200 ml of each bottle is filled. do. After filling the bottle, it is sterilized at 130° C. for 4 to 5 seconds to prepare a drink.

[Formulation Example 4] Granules

8 mg of cornflower extract of any one of Preparation Examples 1 to 4, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed, formed into granules using a fluidized bed granulator, and then filled in a bag to obtain granules to manufacture

[Formulation Example 5] Injection

Injections were prepared in a conventional manner according to the composition shown in Table 8 below.

compounding ingredients content The cornflower extract of any one of Preparation Examples 1 to 4 10-50 mg Sterile distilled water for injection appropriate amount pH adjuster appropriate amount

[Formulation Example 6] Health functional food

Health functional foods were prepared in a conventional manner according to the composition shown in Table 9 below.

compounding ingredients content The cornflower extract of any one of Preparation Examples 1 to 4 20mg vitamin A acetate 70μg vitamin E 1.0mg vitamin B1 0.13mg vitamin B2 0.15mg vitamin B6 0.5mg vitamin B12 0.2μg vitamin C 10mg biotin 10 μg Nicotinamide 1.7mg folic acid 50 μg Calcium Pantothenate 0.5mg ferrous sulfate 1.75mg zinc oxide 0.82mg magnesium carbonate 25.3mg monobasic potassium phosphate 15mg dicalcium phosphate 55mg potassium citrate 90mg calcium carbonate 100mg magnesium chloride 24.8mg

Although the composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, the composition is mixed in a preferred embodiment, but the mixing ratio may be arbitrarily modified.

[Formulation Example 7] Health drink

Health drinks were prepared in a conventional manner according to the composition shown in Table 10 below.

compounding ingredients content The cornflower extract of any one of Preparation Examples 1 to 4 1000mg citric acid 1000mg oligosaccharide 100 g Taurine 1 g Purified water remaining amount

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and sterilized.

[Formulation Example 8] Softening lotion (skin lotion)

A softening lotion was prepared in a conventional manner according to the composition shown in Table 11 below.

compounding ingredients content (% by weight) The cornflower extract of any one of Preparation Examples 1 to 4 0.2 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG-12 Nonylphenyl Ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 9] Nutrient lotion (milk lotion)

Nutrient lotion was prepared in a conventional manner according to the composition shown in Table 12 below.

compounding ingredients content (% by weight) The cornflower extract of any one of Preparation Examples 1 to 4 1.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 Carboxyvinyl Polymer 0.1 beeswax 4.0 Polysorbate 60 1.5 Caprylic/Capric Triglycerides 5.0 squalane 5.0 sorbitasesquioleate 1.5 liquid paraffin 0.5 cetearyl alcohol 1.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 10] Nourishing Cream

Nutrient creams were prepared in a conventional manner according to the composition shown in Table 13 below.

compounding ingredients content (wt%) The cornflower extract of any one of Preparation Examples 1 to 4 2.0 glycerin 3.0 butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 11] Massage Cream

A massage cream was prepared in a conventional manner according to the composition shown in Table 14 below.

compounding ingredients content (wt%) The cornflower extract of any one of Preparation Examples 1 to 4 2.0 glycerin 8.0 butylene glycol 4.0 liquid paraffin 45.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 beeswax 4.0 cetearyl glucoside 1.5 Sesquioleic Acid Sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 12] Pack

A pack was prepared in a conventional manner according to the composition shown in Table 15 below.

compounding ingredients content (wt%) The corn hydrangea of any one of Preparation Examples 1 to 4 0.2 glycerin 4.0 polyvinyl alcohol 15.0 Hyaluronic Acid Extract 5.0 beta glucan 7.0 allantoin 0.1 nonyl phenyl ether 0.4 Polysorbate 60 1.2 ethanol preservative 6.0 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

As described above in detail a specific part of the present specification, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present specification is not limited thereto. Accordingly, the substantial scope of the present specification will be defined by the appended claims and their equivalents.

Claims (13)

Corneous hydrangea ( Hydrangea serrata ) An antibacterial composition for bacteria comprising an extract as an active ingredient, propionibacterium acnes. According to claim 1, wherein the hydrangea is Hydrangea Serrata for acuminata (S. et z.) Wilson (Hydrangea Serrata for. acuminata (Siebold & Zucc) Wilson), Hydrangea Serrata for Bujeri Hydrangea serrata for. buergeri Nakai., Hydrangea serrata for Koreana tea. Lee ( Hydrangea serrata for. coreana T. Lee) and Hydrangea serrata for. fertilis Nakai (Hydrangea serrata for. fertilis Nakai.) at least one composition selected from the group consisting of. The composition of claim 1, wherein the antibacterial is antibacterial against acne bacteria. According to claim 1, wherein the composition is at least one selected from the group consisting of whole cornflower hydrangea, woody leaves, stems, branches, bark, roots, seeds and fruits. The composition of claim 1, wherein the cornflower extract is at least one extract selected from the group consisting of water, an organic solvent, and mixtures thereof. The composition of claim 5, wherein the organic solvent is at least one selected from the group consisting of ethanol, butylene glycol, and propylene glycol. The composition according to any one of claims 1 to 6, wherein the cornflower extract is included in an amount of 0.001 to 50% by weight based on the total weight of the composition. The composition of claim 7, wherein the extract of cornflower hydrangea is included in an amount of 0.01 to 1% by weight based on the total weight of the composition. The composition according to any one of claims 1 to 6, wherein the composition is a composition for external application to the skin. delete delete delete delete