KR102561258B1 - Composition for controlling sebum and improving pore comprising Jasminum Officinale, Salix Alba (willow) bark and Solanum melogena fruit extract - Google Patents

Abstract

It relates to a composition comprising extracts of common jasmine, white willow bark and eggplant fruit, and the composition according to one aspect has a nitric oxide production inhibitory effect, a sebum secretion inhibitory effect, a pore size contraction effect, an anti-inflammatory effect, and a It has an antibacterial effect and is effective in improving, preventing or treating skin conditions.

Description

Composition for controlling sebum and improving pore comprising Jasminum Officinale, Salix Alba (willow) bark and Solanum melogena fruit extract}

It relates to a composition for sebum control and pore improvement comprising extracts of common jasmine, white willow bark and eggplant fruit.

Human skin undergoes changes due to various internal and external factors as we age. Internally, the secretion of various hormones that regulate metabolism is reduced, and the function of immune cells and the activity of cells are lowered, resulting in a decrease in the biosynthesis of constituent proteins necessary for the body. Externally, due to the destruction of the ozone layer, the amount of ultraviolet rays reaching the surface of the sun increases and environmental pollution becomes more serious. As free radicals and active oxygen increase, the thickness of the skin decreases and wrinkles appear. It causes various changes, such as increased skin elasticity, decreased elasticity, and frequent skin troubles.

On the other hand, there are about 20,000 human pores on the face, and the diameter is about 0.02 to 0.05 mm, which sometimes increases due to factors such as seasons and age. In addition, the pores are connected to the sebaceous glands, and serve as passageways for discharging sebum produced by the sebaceous glands. The sebum released through the pores functions to protect the skin from the outside, prevent bacterial infection, and moisturize the skin, but excessive sebum can cause comedos called blackheads or whiteheads. may be Comedones refer to a condition in which sebum is blocked by sebum pores due to repeated activation of sebaceous glands and hyperkeratosis, narrowing of the pores of the hair follicles, hindering the discharge of sebum, and papules, pustules, cysts, and nodules ( Nodule-like inflammatory lesions can progress to acne. In addition, when sebum is excessively secreted, the pores widen in the process of discharging it, and when sebum accumulates in the pores, the pores become wider.

Another reason for enlarged pores is aging of the skin. Aging of the skin affects degeneration or reduction of collagen fibers and elastic fibers that serve to support pores. As a result, as the power to support the pores becomes insufficient, the pores naturally widen. In addition, as skin aging progresses, division of keratinocytes and fibroblasts decreases, collagen synthesis decreases, MMP generation increases, and melanin generation signal transmission increases, etc., which causes wrinkles to increase and skin elasticity. This decreases, and spots, freckles, and age spots increase.

Diet is also one of the factors that affect the size of pores. Eating habits of frequently consuming greasy foods, instant foods, floury foods, sweet foods, or meats cause sebum production, resulting in enlarged pores. As a result, the skin texture becomes rough and a lot of blemishes may occur.

Such enlarged pores not only do not look good, but also cause uneven, uneven skin. In addition, when the pores become large, wastes such as cosmetic residue and bacteria penetrate well, which can cause various skin diseases such as acne. Therefore, it is necessary to discover natural materials that can improve skin conditions, prevent excessive sebum secretion, keep pores clean, prevent the proliferation of harmful bacteria, and effectively manage pore size.

Accordingly, the present inventors have tried to develop a composition for improving skin conditions, and as a result, a complex extract of common jasmine ( Jasminum Officinale ), white willow bark ( Salix Alba (Willow) Bark) and eggplant fruit ( Solanum Melongena (Eggplant) fruit) The present invention was completed by confirming that the sebum secretion can be inhibited and the size of pores can be reduced.

Korea Patent Registration 10-1464914

One aspect is to provide a cosmetic composition for improving or preventing skin conditions comprising a complex extract of common jasmine, white willow bark and eggplant fruit as an active ingredient.

Another aspect is to provide a health food composition for improving or preventing skin conditions comprising a complex extract of common jasmine, white willow bark and eggplant fruit as an active ingredient.

Another aspect is to provide a pharmaceutical composition for improving or treating skin conditions comprising a complex extract of common jasmine, white willow bark and eggplant fruit as an active ingredient.

One aspect provides a cosmetic composition for improving or preventing skin conditions comprising a common jasmine extract, a white willow bark extract, and an eggplant extract as active ingredients.

As used herein, the term "improvement" refers to any action that at least reduces a parameter associated with the condition being treated, eg, the severity of a symptom.

As used herein, the term “prevention” refers to partially or completely delaying or preventing the onset or recurrence of a disease, disorder, or its attendant symptoms, preventing the acquisition or re-acquisition of a disease or disorder, or preventing the occurrence or recurrence of a disease or disorder. Reducing the risk of acquisition. The prevention refers to any action that inhibits or delays the occurrence of inflammation or inflammation-related diseases, disorders, or symptoms by administration of the composition according to the present invention.

The common jasmine is an evergreen shrub, and about 200 species are distributed in the tropics and subtropics, and may be native to the Himalayas. The common jasmine extract is an alkaloid, coumarin, flavonoid, tannin, terpenoid, glycoside, emodine, leucoanthcyanin ), steroids, anthocyanin, phlobatinin, essential oil and saponin.

The white willow may be a species of white willow native to Europe, western and central Asia. The white willow bark extract contains salicin.

The eggplant fruit is a fruit of an elongated or round shape belonging to the solanaceae family, and may be native to India. It is an annual plant in the temperate zone and a perennial plant in the tropics.

The extract may be extracted after mixing common jasmine, white willow bark and eggplant fruit. In general, when extracting after mixing each raw material, the existing efficacy may be impaired. However, in the case of the composite extract prepared by mixing and extracting common jasmine, white willow bark and eggplant fruit according to the present invention, anti-inflammatory effect, skin top bacteria inhibitory effect, pore contraction effect and sebum branching inhibition compared to the original extract alone It was confirmed that the effect was increased.

In addition, according to one embodiment, the extract may be obtained by extracting each of common jasmine, white willow bark, and eggplant fruit, and then mixing them. In the case of preparing a composite extract by mixing the extracted extracts after extracting each raw material, not only can the efficacy of the raw material be maintained, but also the superiority of efficacy can be increased compared to preparing an extract by extracting after mixing the raw material. .

The extract may be extracted from at least one selected from the group consisting of common jasmine, white willow bark, or branches, roots, stems, branches, leaves, seeds, and fruits. The whole or part of the common jasmine, white willow bark or eggplant fruit used for extraction, or materials derived therefrom, may be ground, minced, or dried.

The extraction method for obtaining the extract may be prepared according to known physical, chemical or enzymatic extraction methods commonly used in the art to which the present invention pertains. Specifically, commonly used extraction methods such as cold precipitation, heat extraction, ultrasonic extraction, filtration, pressure extraction, reflux extraction, supercritical extraction, electrical extraction, steam distillation, organic solvent extraction, and hot water extraction may be used, and conventional extraction equipment may be used. , sonicating extractor or fractionator can be used.

In one embodiment, in the case of extraction using the hot water extraction method, a solvent is added to the raw material and extracted at a temperature of 80 to 100 ° C for 1 to 5 hours, and then 15 to 25 ° C, for example, 16 to 25 ° C, It may be cooled at 17 to 25 °C, 17 to 24 °C, 27 to 23 °C or room temperature. In addition, the hot water extraction may be repeated 1 to 5 times. The hot water export method is useful for obtaining a large amount of extract in a short period of time, and the active ingredient content significantly increases as the extraction time and temperature increase.

In one embodiment, the extract may be extracted using water, alcohol, or a mixture thereof as a solvent. The alcohol may be a C1 to C6 alcohol, for example a C1 to C4 alcohol. The C1 to C6 alcohol may be methanol, ethanol, propanol, isopropanol, 1,3-propanediol, butanol, pentanol, hexanol or butylene glycol.

In one embodiment, the extraction is 3 to 10 (volume or weight) times the extraction solvent for each extract, for example, 3 to 7 (volume or weight) times, 3 to 5 (volume or weight) times , 5 to 10 (volume or weight) times, or 4 to 10 (volume or weight) times.

In one embodiment, the solvent may be a mixture of water and alcohol, that is, an aqueous alcohol solution, for example, an aqueous ethanol solution. The alcohol concentration in the alcohol aqueous solution is 1 to 99.5 (v / v)%, 10 to 99.5 (v / v)%, 1 to 70 (v / v)%, 1 to 40 (v / v)%, 5 to 25 (v/v)%, 7 to 20 (v/v)%, 5 to 25 (v/v)%, or 10 to 20 (v/v)%.

In one embodiment, the common jasmine, white willow bark and eggplant extract may be included in a weight ratio of 1: 0.1 to 10: 0.1 to 10. For example, 1: 0.5 to 10: 0.5 to 10, 1: 0.5 to 9: 0.5 to 9, 1: 0.5 to 8: 0.5 to 8, 1: 0.5 to 7:0.5 to 7, 1:0.5 to 6:0.5 to 6, 1:0.5 to 5:0.5 to 5, 1:0.6 to 5:0.6 to 5, 1:0.7 to 5:0.7 to 5, 1:0.8 to 5:0.8 to 5, 1:0.9 to 5:0.9 to 5, 1:1 to 5:1 to 5, 1:1 to 5:1 to 4, 1:1 to 5:1 to 3 or 1:1 to 4: It may be included in a weight ratio of 1 to 2.

In one embodiment, the composite extract is 0.0001% to 99.0% by weight relative to the total weight of the composition, for example, 0.01% to 60% by weight, 0.01% to 40% by weight, 0.01% to 30% by weight, 0.01% to 20% by weight, 0.01% to 10% by weight, 0.01% to 5% by weight, 0.05% to 60% by weight, 0.05% to 40% by weight, 0.05% to 30% by weight, 0.05% by weight % to 20% by weight, 0.05% to 10% by weight, 0.05% to 5% by weight, 0.1% to 60% by weight, 0.1% to 40% by weight, 0.1% to 30% by weight, 0.1% to 0.1% by weight 20 wt%, 0.1 wt% to 10 wt%, 1 wt% to 50 wt%, 1 wt% to 40 wt%, 2 wt% to 40 wt%, 3 wt% to 30 wt%, 3 wt% to 20 wt% %, 3 wt% to 18 wt%, 3 wt% to 15 wt%, 3 wt% to 15 wt%, or 3 wt% to 10 wt% may be included.

In addition, if necessary, after the above extraction, filtration and/or concentration and/or freeze-drying methods known in the art may be additionally used.

In one embodiment, the skin condition improvement may be suppression of skin inflammation, enhancement of skin defense, skin exfoliation, improvement of skin wrinkles, improvement of skin elasticity, inhibition of skin aging, removal of sebum, improvement of acne, or reduction of pore size.

In one embodiment, the complex extract may improve skin conditions by inhibiting nitric oxide (NO) production.

The composite extract may have antibacterial activity against skin flora.

In one embodiment, the skin flora is Propionibacterium acnes , Staphylococcus aureus , Streptococcus pyogenes , Corynebacterium striatum ) and Staphylococcus epidermidis ( Staphylococcus epidermidis ) It may be any one or more selected from the group consisting of.

The cosmetic composition may be prepared in any formulation commonly prepared in the art to which the present invention pertains. For example, it may be formulated into softening lotion, nutrient lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc. It is not limited. More specifically, skin lotion, skin softener, skin toner, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulations.

The cosmetic composition may include additional ingredients commonly used in cosmetics, such as thickeners, dispersants, fragrances, fillers, preservatives, preservatives, neutralizers, sweeteners, vitamins, free-radical scavengers, metal ion sequestering agents, functional ingredients and these It may further include any conventional cosmetic ingredient which may be selected from mixtures of One skilled in the art may select any additional ingredients and/or amounts thereof such that the beneficial properties of the compositions according to the present disclosure are not adversely affected or substantially unaffected by the anticipated addition.

When the cosmetic composition is a surfactant-containing cleansing formulation, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester may be further included.

If the cosmetic composition according to the present invention is a cream or gel formulation, it may further contain animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters and the like may be further included.

When the cosmetic composition is a suspension formulation, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may further be included.

When the cosmetic composition is in a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, additionally chlorofluorohydrocarbon , a propellant such as propane/butane or dimethyl ether.

The cosmetic composition may be used by applying alone or overlapping, or by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

Another aspect provides a health food composition for improving or preventing skin conditions comprising a common jasmine extract, a white willow bark extract, and an eggplant extract as active ingredients.

The common jasmine extract, white willow bark extract and eggplant extract are as described above. In addition, the skin condition is as described above.

In this specification, the term "health functional food" refers to a food manufactured and processed by using a specific ingredient as a raw material or by extracting, concentrating, refining, mixing, etc. a specific ingredient contained in a food raw material for the purpose of health supplement. It refers to food designed and processed so that it can sufficiently exert biological control functions such as biological defense, regulation of biological rhythm, prevention and recovery of disease, etc. The health functional food composition may perform functions related to prevention and improvement of skin damage caused by fine dust.

There is no particular limitation on the type of food. Examples of foods to which the extract may be added include powders, granules, tablets, capsules, pills, gels, jellies, suspensions, emulsions, syrups, tea bags, leached teas, gums, candies, and formulations selected from the group consisting of health drinks etc., and includes all health foods in the usual sense.

The health beverage composition may include various sweeteners, flavoring agents, or natural carbohydrates as additional components, as in conventional beverages. The sweetener may be a natural sweetener or a synthetic sweetener. The natural sweetener may be taumatin or stevia extract, and the synthetic sweetener may be saccharin or aspartame.

The natural carbohydrate may be monosaccharide, disaccharide, polysaccharide, xylitol, sorbitol or erythritol. The monosaccharide may be glucose or fructose, and the disaccharide may be maltose or sucrose. The polysaccharide may be a dextrin or a cyclodextrin. The proportion of the natural carbohydrate may be generally about 0.01 to 10 g, for example, about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

The health functional food may include food additives acceptable in food science, and may include appropriate carriers commonly used in the manufacture of health functional food.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages; and the like. In addition, the composition of the present invention may include fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not critical, but is generally selected in the range of 0.01 to 0.1 part by weight per 100 parts by weight of the composition of the present invention.

Another aspect provides a pharmaceutical composition for preventing or treating inflammatory skin diseases or for antibacterial use, comprising a common jasmine extract, a white willow bark extract, and an eggplant extract as active ingredients.

As used herein, the term "treatment" refers to any action that improves or beneficially alters a disease, disorder, or its attendant symptoms.

The common jasmine extract, white willow bark extract and eggplant extract are as described above. In addition, the skin condition is as described above.

The inflammatory skin disease is selected from the group consisting of dermatitis, allergic dermatitis, irritant dermatitis, seborrheic dermatitis, atopic dermatitis, sensitive skin disease, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, drug rash and acne It can be any one.

In one embodiment, the pharmaceutical composition may be formulated in a formulation selected from the group consisting of tablets, soft or hard capsules, pills, powders, suspensions, syrups, injections and granules.

In one embodiment, the pharmaceutical composition may be for oral or parenteral administration.

The pharmaceutical composition includes conventional fillers, extenders, binders, disintegrants, anti-agglomerates, lubricants, wetting agents, pH adjusters, nutrients, vitamins, electrolytes, alginic acid and its salts, pectic acid and its salts, protective chlorides, glycerin, Perfumes, emulsifiers or preservatives may be included.

The pharmaceutical composition may include a pharmaceutically acceptable carrier, examples of which include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, and gelatin. , calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, propylhydroxybenzoate, It may be at least one selected from the group consisting of talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin and physiological saline.

The formulation of the pharmaceutical composition may vary depending on the method of use, and is formulated using a method well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. It can be.

Formulations for oral administration include tablets, soft or hard capsules, pills, powders, suspensions, syrups, injections and granules, etc., and these formulations include one or more excipients such as starch, calcium carbonate, sucrose or lactose. , It may be prepared by mixing gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Formulations for parenteral administration may be creams, lotions, ointments, warnings, liquids, aerosols, liquid extracts, elixirs, salivas, sachets, patches, or injections.

The method is applicable to any animal, and the animal may include livestock such as cattle, pigs, sheep, horses, dogs, and cats, as well as humans and primates.

The dosage of the composition for treatment, prevention, or improvement may be determined in consideration of the administration method, the age, sex, severity of the patient, the degree of absorption of the active ingredient in the body, the inactivation rate, and concomitant drugs, 0.1 mg/kg (body weight) to 500 mg/kg (body weight), 0.1 mg/kg (body weight) to 400 mg/kg (body weight), or 1 mg/kg (body weight) to 300 based on the daily active ingredient It may be administered in mg/kg (body weight), and may be administered once or divided into several times, but is not limited thereto.

The composition according to one aspect has an effect of inhibiting nitric oxide production, inhibiting sebum secretion, pore size contraction, anti-inflammatory effect, and antimicrobial effect against skin flora, thereby improving, preventing or treating skin conditions.

1 is a graph showing the change in pore size after using the essence.
Figure 2 is a graph showing the improvement rate for pore size after using the essence according to Preparation Example 6 compared to the pore size before using the essence according to Preparation Example 6: the test product is the essence according to Preparation Example 6; The control product is the essence according to the control group.

It will be described in more detail through the following examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1. Preparation of Complex Extracts of Common Jasmine, White Willow Bark and Eggplant Fruit

To prepare a composite extract of common jasmine, white willow bark and eggplant, common jasmine flowers, white willow bark and eggplant were washed with distilled water and then dried. Then, according to the mixing ratio of Table 1 below, 25 g of the common jasmine flower, 25 g of white willow bark, and 25 g of eggplant fruit were mixed, and then 30% by weight of butylene glycol 150 as an extraction solvent in the mixture mL was added and hot water extraction was performed at a temperature of 85 to 100 °C for 2 hours a total of 3 times. And after cooling the extract at room temperature, the precipitate was filtered with Whatman filter paper from 0.8 μm to 0.2 μm to prepare a composite extract of common jasmine, white willow bark and branch fruit mixture.

weight ratio common jasmine white willow bark eggplant fruit Example 1 One One One

Examples 2 to 6. Preparation of Complex Extracts of Common Jasmine Extract, White Willow Bark Extract and Eggplant Fruit Extract

To prepare a composite extract of common jasmine extract, white willow bark extract and eggplant extract, common jasmine flowers, white willow bark and eggplant fruit were washed with distilled water and then dried. Thereafter, 150 mL of 30 wt% butylene glycol as an extraction solvent was added to 25 g of the common jasmine flower, and hot water extraction was performed three times at a temperature of 85 to 100 ° C. for 2 hours to obtain a common jasmine flower extract. After cooling the common jasmine flower extract at room temperature, the precipitate was filtered with a Whatman filter paper from 0.8 μm to 0.2 μm to prepare a common jasmine extract. In the same manner, using 25 g of white willow bark and 25 g of eggplant fruit, extracts of white willow bark and eggplant fruit were prepared, respectively. Thereafter, the extracts of common jasmine, white willow bark and eggplant were mixed according to the mixing ratio shown in Table 1 below to prepare a composite extract of common jasmine, white willow bark and eggplant.

weight ratio common jasmine white willow bark eggplant fruit Example 2 One One One Example 3 2 One 3 Example 4 2 3 One Example 5 One 2 3 Example 6 One 3 2

Comparative Examples 1 to 3. Preparation of Common Jasmine Extract, White Willow Bark Extract and Eggplant Fruit Extract

To prepare extracts of common jasmine, white willow bark and eggplant, common jasmine flowers, white willow bark and eggplant were washed with distilled water and then dried. Thereafter, 150 mL of 30 wt% butylene glycol as an extraction solvent was added to 25 g of the common jasmine flower, and hot water extraction was performed three times at a temperature of 85 to 100 ° C. for 2 hours to obtain a common jasmine flower extract. After cooling the common jasmine flower extract at room temperature, the precipitate was filtered with a Whatman filter paper from 0.8 μm to 0.2 μm to prepare a common jasmine extract. In the same manner, using 25 g of white willow bark and 25 g of eggplant fruit, extracts of white willow bark and eggplant fruit were prepared, respectively. And the compounding ratio is shown in Table 3.

weight ratio common jasmine white willow bark eggplant fruit Comparative Example 1 One 0 0 Comparative Example 2 0 One 0 Comparative Example 3 0 0 One

Comparative Examples 4 to 6. Preparation of mixed extract of common jasmine and white willow bark, mixed extract of common jasmine and eggplant fruit, and mixed extract of white willow bark and eggplant fruit

To prepare common jasmine and white willow bark mixed extract, common jasmine and eggplant mixed extract, and white willow bark and eggplant mixed extract, common jasmine flowers, white willow bark and eggplant were washed with distilled water, and then dried Thereafter, 150 mL of 30 wt% butylene glycol as an extraction solvent was added to 25 g of the common jasmine flower, and hot water extraction was performed three times at a temperature of 85 to 100 ° C. for 2 hours to obtain a common jasmine flower extract. After cooling the common jasmine flower extract at room temperature, the precipitate was filtered with a Whatman filter paper from 0.8 μm to 0.2 μm to prepare a common jasmine extract. In the same manner, using 25 g of white willow bark and 25 g of eggplant fruit, extracts of white willow bark and eggplant fruit were prepared, respectively. Then, the common jasmine, white willow bark and eggplant extracts were mixed according to the mixing ratio shown in Table 2 below to obtain common jasmine and white willow bark mixed extract, common jasmine and eggplant mixed extract, and white willow bark and eggplant. A mixed fruit extract was prepared.

weight ratio common jasmine white willow bark eggplant fruit Comparative Example 4 One One 0 Comparative Example 5 0 One One Comparative Example 6 One 0 One

Preparation Examples 1 to 6. Preparation of Essence Formulation

Essence was prepared by mixing the ingredients in Table 5 below. The contents of the components are expressed in weight %.

ingredient control group Comparative Preparation Example 1 Comparative Preparation Example 2 Comparative Preparation Example 3 Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 Preparation Example 5 Preparation Example 6 Comparative Example 1 - 5 - - - - - - - - Comparative Example 2 - - 5 - - - - - - - Comparative Example 3 - - - 5 - - - - - - Example 1 - - - - 5 - - - - - Example 2 - - - - - 5 - - - - Example 3 - - - - - - 5 - - - Example 4 - - - - - - - 5 - - Example 5 - - - - - - - - 5 - Example 6 - - - - - - - - - 5 Jintan gum 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Hydroxyethyl Cellulose 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 glycerin 2 2 2 2 2 2 2 2 2 2 sodium hyaluronate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 1,2-hexanediol 2 2 2 2 2 2 2 2 2 2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1. Measurement of antioxidant activity

In order to measure the degree of antioxidant of the extracts prepared in Examples 1 to 6 and Comparative Examples 1 to 6, radical scavenging activity was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl).

Specifically, 0.9 mL of a 4.1 x 10 ^-5 M DPPH solution was added to 0.1 mL of the 0.1%, 0.5% or 1% concentration of the extract, reacted at room temperature for 30 minutes, and then absorbance was measured at 517 nm. As a control, ascorbic acid was used. Absorbance was measured three times in the same manner as above, and radical scavenging activity was calculated using Equation 1 below. And the average value of the radical scavenging ability for each extract obtained by repeating the experiment three times is shown in Table 6:

Equation 1

DPPH radical scavenging ability (%) = 100 - [(absorbance of sample / absorbance of control group)Х100].

division DPPH radical scavenging activity (%) Concentration of extract or control 0.1% 0.5% One % Comparative Example 1 37.7 54.4 56.2 Comparative Example 2 57.8 58.2 63.4 Comparative Example 3 49.3 54.4 58.3 Comparative Example 4 65.2 66.8 68.1 Comparative Example 5 55.2 56.6 58.6 Comparative Example 6 40.1 43.5 45.8 Example 1 56.2 60.2 63.8 Example 2 60.5 62.7 63.4 Example 3 54.2 57.8 62.7 Example 4 67.6 68.5 70.6 Example 5 60.3 62.3 64.8 Example 6 70.8 73.6 75.6 Ascorbic acid (control) 87.0 90.9 92.2

As shown in Table 6, the composite extract of common jasmine, white willow bark and eggplant extract is any one extract selected from the group consisting of common jasmine extract, white willow bark extract and eggplant extract, or a mixture of two extracts. It was confirmed that the radical scavenging activity was markedly contrasted.

Experimental Example 2. NO production inhibition effect measurement

In order to compare the anti-inflammatory efficacy of the extracts prepared in Examples 1 to 6 and Comparative Examples 1 to 6, the NO production inhibitory effect was measured.

Specifically, RAW264.7 macrophages were dispensed into a 96 well plate at 3.0 Х 10 ⁴ cells/well and cultured for 24 hours, followed by 0.1%, 0.5% or 1% extract and 1 μg/mL LPS (lipopolysaccharide). and incubated for 24 hours. In addition, 50 μL of Griess reagent (1% sulfanilamide / 0.1% naphthylethylene diamine dihydrochloride / 2% phosphoric acid, SIGMA) was mixed with 50 μL of the culture medium, reacted for 15 minutes, and absorbance was measured at 540 nm. A standard calibration curve was prepared with sodium nitrate, and NO ₂ concentration was calculated. All experiments were repeated three times, and the results are shown in Table 7 as an average.

division NO production inhibition effect (%) Concentration of extract or control 0.1% 0.5% One % Comparative Example 1 16.7 20.0 25.7 Comparative Example 2 37.9 42.4 44.8 Comparative Example 3 15.32 18.6 20.3 Comparative Example 4 37.9 42.4 51.8 Comparative Example 5 31.7 41.6 47.6 Comparative Example 6 30.3 34.5 36.1 Example 1 42.5 48.6 52.6 Example 2 40.6 45.8 48.1 Example 3 50.5 54.2 58.9 Example 4 60.1 62.6 64.8 Example 5 56.8 59.8 62.4 Example 6 65.64 68.1 72.6 Ascorbic acid (control) 78.68 98.53 98.69

As shown in Table 7, it was confirmed that the composite extract of common jasmine, white willow bark, and eggplant fruit showed an NO production inhibitory effect of 40% or more at a concentration of 0.1%. In particular, in the case of the extract according to Example 1.6, it was confirmed that the NO production inhibitory effect of 65.64% was exhibited, showing the best NO production inhibitory effect. On the other hand, in the case of a single extract or a mixed extract composed of two extracts, it was confirmed that at a concentration of 0.1%, the NO production inhibitory effect was around 30%. Therefore, the composite extract of common jasmine, white willow bark, and eggplant extract has significant anti-inflammatory efficacy compared to any one extract selected from the group consisting of common jasmine extract, white willow bark extract, and eggplant extract, or a mixture of two extracts. It was confirmed that

Experimental Example 3. Measurement of skin flora growth inhibitory effect

In order to measure the effect of inhibiting the growth of skin flora of the extracts prepared in Examples 1 to 6 and Comparative Examples 1 to 6, Propionibacterium acnes, which is one of the skin flora and is a major microbiological cause of acne, P. acnes ) and The antibacterial activity against Staphylococcus epidermidis ( S. epidermidis ) was measured through a disc diffusion test.

Specifically, P. acnes and S. epidermidis strains were isolated on a plate medium and incubated at 37° C. for 48 hours to activate them. Then, a single colony activated in the medium was taken with a disposable loop and suspended in RCM broth and TSA broth to a 0.5 McFarland standard turbidity concentration (1×10 ⁸ CFU/mL) to prepare a stock culture. In addition, the stock culture was diluted 10 times to a concentration of 1 × 10 ⁷ CFU/mL to prepare a working culture, and then 0.1 mL of the working culture was dispensed on a plate medium and uniformly spread with a disposable spreader. . Then, 8 mm of sterilized filter paper dics were placed on the surface of the plate medium, and then 0.05 mL/disc of the extract at a concentration of 50 mg/mL was dispensed. After drying at room temperature so that the extract was well absorbed, it was anaerobically cultured for 48 hours under 37 ℃ conditions in an anaerobic jar using an anaerobic gas kit (anerobic gas kit). The above process was repeated three times, and the diameter of the growth-inhibited ring (clear zone, mm) generated on each disc after culture was measured. The results were expressed as average values and are shown in Table 8.

division Growth inhibition circle (mm) target strain Propionibacterium acnes ( P. acnes ) Staphylococcus epidermidis ( S. epidermidis ) Comparative Example 1 - - Comparative Example 2 - - Comparative Example 3 - - Comparative Example 4 - - Comparative Example 5 - 9 Comparative Example 6 9 - Example 1 9 13 Example 2 11 9 Example 3 13 14 Example 4 10 16 Example 5 11 13 Example 6 11 17 Hexanediol (control) 18 20

As shown in Table 8, it was confirmed that the composite extract of common jasmine, white willow bark, and eggplant fruit exhibited antibacterial activity against P. acnes and S. epidermidis strains. On the other hand, it was confirmed that each of the common jasmine extract, the white willow bark extract, and the eggplant extract did not exhibit antibacterial activity against P. acnes and S. epidermidis strains. In addition, the two mixed extracts selected from the group consisting of common jasmine extract, white willow bark extract and eggplant extract exhibit antibacterial activity against only one of P. acnes and S. epidermidis strains, or against the above two strains. It was confirmed that there was no antibacterial activity against all.

This is because the complex extract of common jasmine, white willow bark and eggplant fruit has excellent skin flora growth compared to any one extract selected from the group consisting of common jasmine extract, white willow bark extract and eggplant extract or a mixture of two extracts. means to have an inhibitory effect.

Experimental Example 4. Pore contraction effect

In order to measure the pore contraction effect of the extracts prepared in Examples 1 to 6. and Comparative Examples 1 to 6., the pore contraction effect was measured using albumin as a protein to replace the skin.

Specifically, after dissolving 0.3% albumin in 0.05 M citric acid buffer (pH 4.5), 1 mL of 1%, 5% or 10% extract was added to 2 mL of 0.3% albumin solution. And after stirring for 5 minutes, the absorbance was measured by UV Visible spectrum at 650 nm. As a control, 1 mL of an extraction solvent was added instead of the extract. The measurement results are shown in Table 9 below, and the higher the absorbance value, the better the pore shrinking function. Each Comparative Example and Example was diluted to a concentration of 5% by adding purified water.

division Absorbance (A.U.) Concentration of extract or control One % 5% 10% Comparative Example 1 0.3653 0.6869 0.9528 Comparative Example 2 0.1365 0.3758 0.6135 Comparative Example 3 0.2368 0.3487 0.5336 Comparative Example 4 0.6968 0.9658 1.1053 Comparative Example 5 0.4568 0.5814 0.6589 Comparative Example 6 0.7864 1.0363 1.2356 Example 1 0.5698 0.5896 0.6862 Example 2 0.8624 1.3664 1.5986 Example 3 1.2658 1.5616 1.7969 Example 4 1.6891 1.8964 2.3689 Example 5 0.8694 1.4638 1.6968 Example 6 1.2316 1.8968 1.9845 control group 0.0064

As shown in Table 9, the composite extract of common jasmine, white willow bark, and eggplant extract is any one extract selected from the group consisting of common jasmine extract, white willow bark extract, and eggplant extract, or a mixture of two extracts. In contrast, it was confirmed that a remarkable pore contraction effect was shown.

Experimental Example 5. Sebum secretion inhibitory effect

The sebum secretion inhibitory effect of the essences of Preparation Examples 1 to 6 was measured by using a skin oil meter (Sebum meter SM 810) to measure the sebum secretion of the skin of 24 test subjects.

Specifically, 24 men and women between the ages of 20 and 40 were divided into eight groups (groups A to I) of three each regardless of gender. In addition, the essence according to the manufacturing example was applied to the left part of the forehead of each group member twice a day in the morning and evening, and the essence containing purified water was applied to the right part of the forehead twice a day in the morning and evening. Then, at 2 weeks and 4 weeks after application, the amount of sebum secreted by the skin was measured using a skin oil meter. The experiment was conducted under conditions of 25 °C and 20% relative humidity. The experimental results are shown in Table 10 as the average values of each group.

Normal skin has a value of 100 to 220 μg/cm 2 and for oily skin, the measured value is 220 μg/cm 2 or more.

group formulation Sebum secretion amount before implementation (μg/cm2) Sebum secretion after 14 days (μg/cm2) Sebum secretion amount after 28 days (μg/cm2) Improvement after 28 days (%) Group A Comparative Preparation Example 1 223 215 214 4.03 control group 221 210 226 -2.26 group B Comparative Preparation Example 2 216 213 211 2.31 control group 217 218 217 0 C group Comparative Preparation Example 3 221 210 209 5.43 control group 223 220 225 -0.90 D group Preparation Example 1 224 203 198 11.6 control group 221 218 220 0 group E Preparation Example 2 233 218 216 7.30 control group 230 227 229 0.43 Group F Preparation Example 3 221 194 193 12.67 control group 224 221 218 2.67 G group Production Example 4 220 198 197 10.45 control group 217 208 211 2.76 H group Preparation Example 5 224 201 202 9.82 control group 223 221 221 0.90 group I Preparation Example 6 221 196 189 14.48 control group 223 210 223 0

As shown in Table 10, it was confirmed that the formulation according to Preparation Example significantly decreased the amount of sebum secretion compared to the control group 28 days after application. In addition, it was confirmed that all of the formulations according to Preparation Examples 1 to 6 showed an improvement effect of about 2 times or more compared to the formulations according to Comparative Preparation Examples 1 to 3 in the degree of improvement in the amount of sebum secretion. In particular, it was confirmed that the formulation according to Preparation Example 6 showed a significant sebum improvement effect by showing a sebum secretion amount of 189 μg/cm 2 and a degree of improvement of 14.48% 28 days after application.

Experimental Example 6. Pore improvement effect

In order to measure the pore-improving effect of the essence according to Preparation Example 6, the change in pores was confirmed using ANTERA 3D (Miravexn Limited, Ireland) for 20 adult female subjects.

Specifically, the essence was applied to the cheeks of the face, and the change in pores was measured after 2 weeks and 4 weeks after using the essence, and the results are shown in FIGS. 1 and 2. Specifically, when the essence according to Preparation Example 6 was applied, it was confirmed that the size of pores initially 0.487 mm³ became 0.373 mm³ after 4 weeks of use. In particular, when the essence according to the control group was applied, the pore size increased from 0.514 mm³ at the beginning to 0.526 mm³ after 4 weeks, compared to the fact that the essence according to the manufacturing example showed a significant pore improvement effect (p <0.05) .

In addition, when the essence according to Preparation Example 6 was used for 4 weeks, it was confirmed that the pore improvement rate was 26.648% compared to the pores before use, indicating a significant pore improvement rate compared to the essence according to the control group.

1 is a graph showing the change in pore size after using the essence.

Figure 2 is a graph showing the improvement rate for pore size after using the essence according to Preparation Example 6 compared to the pore size before using the essence according to Preparation Example 6: the test product is the essence according to Preparation Example 6; The control product is the essence according to the control group.

Claims (12)

Common jasmine flower ( Jasminum Officinale flower), white willow bark ( Salix Alba (Willow) Bark) and Solanum Melongena (Eggplant) fruit as an active ingredient, a cosmetic composition for improving or preventing skin conditions,
The improvement of the skin condition is to suppress skin inflammation, remove sebum, improve acne or reduce pore size, a cosmetic composition. delete The method of claim 1,
The composite extract is a cosmetic composition prepared by mixing common jasmine flowers, white willow bark and eggplant fruits and then extracting them. The method of claim 1,
The composite extract is a cosmetic composition prepared by extracting common jasmine flowers, white willow bark and eggplant fruits, respectively, and then mixing them. The method of claim 1,
A cosmetic composition comprising the common jasmine flower, white willow bark and eggplant extract in a weight ratio of 1: 0.1 to 10: 0.1 to 10. The method of claim 1,
The composite extract is a cosmetic composition that is included in 0.0001% to 99.0% by weight relative to the total weight of the composition. The method of claim 1,
The composite extract is to inhibit the production of nitric oxide (nitric oxide: NO), a cosmetic composition. The method of claim 1,
The composite extract has an antibacterial activity against acne-causing bacteria, a cosmetic composition. The method of claim 8,
The acne-causing bacteria are Propionibacterium acnes ( Propionibacterium acnes ) or Staphylococcus epidermidis ( Staphylococcus epidermidis ), cosmetic composition. A health food composition for improving or preventing skin conditions comprising complex extracts of Jasminum Officinale flower, Salix Alba (Willow) Bark, and Solanum Melongena (Eggplant) fruit as active ingredients as,
The skin condition improvement is to suppress skin inflammation, remove sebum, improve acne or reduce pore size, the health food composition. For prevention or treatment of inflammatory skin diseases containing complex extracts of Jasminum Officinale flower, Salix Alba (Willow) Bark and Solanum Melongena (Eggplant) fruit as active ingredients, Or a pharmaceutical composition for antibacterial acne causative bacteria. The method of claim 11,
The inflammatory skin disease is selected from the group consisting of dermatitis, allergic dermatitis, irritant dermatitis, seborrheic dermatitis, atopic dermatitis, sensitive skin disease, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, drug rash and acne Any one, a pharmaceutical composition.