KR20140115228A - Composition for prevention or treatment of hair loss comprising oriental herbal extracts - Google Patents

Abstract

The present invention relates to a composition for preventing or treating hair loss, which comprises a mixed extract of Phellinus linteus, Acanthopanax senticosus, and Sepharose as an active ingredient. The mixed extracts of Phellodendron, Spodoptera litura, and Sepharose according to the present invention are biostable and have excellent hair growth promoting effect, and thus can be usefully used as a composition for preventing or treating hair loss.

Description

[0001] The present invention relates to a composition for prevention or treatment of hair loss comprising an extract of a herb medicine as an active ingredient,

The present invention relates to a composition for preventing or treating hair loss, which comprises a mixed extract of Phellinus linteus, Acanthopanax senticosus, and Sepharose as an active ingredient.

The human body has about 100,000 to 150,000 hairs, and each hair has a different cycle and repeats anagen, catagen, and telogen. During the growing season hair grows to make keratin, and the regressive phase grows slowly as the growth phase ends and the metabolic process slows down while maintaining the shape of the hair. In the retrograde period, keratin is not made, and the cell division of the hair follicle is stopped, and it is a period of shrinking rapidly. At the next resting period, the hair follicle stops its activity completely and prepares for hair loss. It is pushed by the newly growing hair at the base of the resting hair, falls off by itself, and repeats the cycle of withdrawal by physical action such as combing or winding hair. This cycle repeats over 3 to 6 years, resulting in a normal drop in hair around 100 per day.

As described above, since human hair has a mother cycle, it always maintains a constant number of hairs without molting. However, when the baldness progresses, the nipples in the hair follicles become smaller, and when the papillae become smaller, the thickness of the hair becomes smaller, and at the same time, the wool becomes shorter, and the newly emerged hair becomes thinner. Therefore, as the baldness progresses, the hair turns into fluffy hair, and the mother period becomes shorter, so that it grows a little and then falls off. As the baldness progresses, the scalp becomes more oily and seborrheic dermatitis develops, and the sebaceous glands attached to the hair follicle gradually grow to produce sebum, which is not the cause of baldness but secondary phenomenon of baldness. .

From time to time, hair loss has been widely recognized as a common disease in middle- and high-middle-aged men. However, in the modern society, middle-aged men as well as women, and even teenagers, are increasing hair loss, which is becoming a serious problem in recent years.

The causes of hair loss are largely hereditary, and genetic causes include disproportionate secretion of hormones, mental stress of social life, exposure to air pollution, intake of processed foods, serious illness due to high fever, nutritional imbalance, A variety of habits and environments such as thyroid medications, oral contraceptives, shampoo, strong ultraviolet rays, sweating during exercise, eating habits, and psychological pressure are known to be the main culprits.

Currently, many anti-hair-loss agents or hair-removing agents for preventing hair loss and promoting hair are available on the world market, but there are still few drugs or technologies that can satisfy the therapeutic effect. The US Food and Drug Administration (FDA), the only drug approved by the Food and Drug Administration (FDA) for the treatment of hair loss, is minoxidil, a product developed as a hypertensive drug originally developed for the treatment of hypertension. Baldness and alopecia. Another drug currently in use is Propecia, which is based on the hormone hypothesis of hair loss. The male hormone is converted to the metabolite dihydrotestosterone (DHT) by the action of 5-alpha reductase The material is known to atrophy and destroy hair follicles. The propecia inhibits DHA production by blocking 5-alpha-reductase, thereby slowing the progression of male-type hair loss to the utmost. The effect of the drug is not on hair growth but on preventing hair loss.

On the other hand, white porcelain ( Thujae orientalis ) is a seed of Thuja orientalis L. It is egg- shaped to long elliptical or long column-shaped, and has a length of 3 to 5 mm and a diameter of 2 to 3 mm. Outer surface is pale yellow to yellowish white, old ones gradually change color to yellowish brown, and oil comes out. The outer surface of the seed is often wrapped in a thin film-like endosperm, the tip is usually pointed, and the root is brown and has a dark brown point. The lower part is a dull circular shape. The cross section of this herb is microscopically white to yellowish white, rich in endosperm, and rich in lipids (Korean Pharmacopoeia and herbal medicine specifications). In addition, the Thuja orientalis L. varieties are 25cm in height and 1m in diameter, and the bark is reddish brown or grayish brown in color. The young branch is flat in green. Leaves are scaly and arranged in layers of V or X shape with a width of 2 ~ 2.5mm and a small line on the back. It is difficult to distinguish front and back. One male and two male flowers running at the end of last year are about 2 ~ 2.5mm long and brown. It consists of ten scales. The female flower is made up of 8 pieces of scales and is bent backward. It is light brown and spherical. The fruit is ovate with a cone and it is like a rugged and horny shape. It is ripened from green to brown as if covered with white powder from September to November, and there are 6 to 8 pieces of fruit. The seeds are 2 ~ 3 pieces in one piece, oval and dark brown. It is difficult to distinguish between the front and back of the leaf, unlike a flower or a flower. White porcelain is used to stabilize the mind and body and to lubricate the intestines, and is used for border, sperm, huhan, and constipation.

Aacanthopanax senticosus is a deciduous broad- leaved shrub of the Araliaceae Araliaceae, 2 to 3 m high. Thick and long thorns are dense throughout and grayish brown. Especially, there are many thorns on the bottom of petiole. Leaves are palmate folded leaves with alternate phyllotaxis and 3-5 small leaves with inverted ovate or long oval shape. There are hairs on the surface and there are brown hairs on the veins when the child is young and there are sharp tooth-like sawtooth on the edge. The petiole is 3 ~ 8㎝ long and has many thorns. The flowers bloom in July with light purple and yellow, and the inflorescence is run one at the end of the branch or split at the bottom. There is a honey gland where the peduncle is split. The style is 1 to 1.8 cm in length, fully merged, and the head of the stigma is very shallowly divided into five branches. The fruit is round and has a diameter of 8 ~ 10mm and ripens in black in September. Acanthopanax is used as neuralgia, arthritis, hypertension, nervous breakdown, diabetes and tonic.

Seoho is a perennial herbaceous herbaceous perennial herbaceous plant, also known as lepidoptera, lepidoptera. It grows in the grass and is 40 ~ 70cm high. There are no hairs in the whole aeration, and the branches are divided on the thin long stem. Roots are thick and short. The leaves are staggered, line-shaped or bar-shaped, the edges are flat, and the veins are parallel. The leaf on the root is like petiole with its lower part narrowed, and its length is 10 ~ 30cm. The leaves on the stem are 4 ~ 10cm long, 5 ~ 15mm long and the tip is pointed. Seoho is used for fever, pain, tonic, respiratory, digestive, and circulatory diseases.

As described above, although various pharmacological effects of white porcine, acanthopanax senticosus and safflower are known, there has been no report on the effect of accelerating the hair growth of the mixed extracts of white porcine, acanthopanax senticosus and safflower, .

Accordingly, the inventors of the present invention conducted a study to develop a novel composition for prevention or treatment of hair loss, and as a result, it was confirmed that the mixed extract of white porcine, Respectively.

It is an object of the present invention to provide a pharmaceutical composition for prevention or treatment of hair loss, which comprises, as an active ingredient, a mixed extract of Phellodendron, Spodoptera litura, and Seicho.

It is still another object of the present invention to provide a composition for preventing or alleviating hair loss, which comprises a mixed extract of Phellodendron, Spodoptera litura, and Sepharose as an active ingredient.

It is still another object of the present invention to provide a cosmetic composition for preventing or improving hair loss, which comprises a mixed extract of Phellodendron, Acanthopanax senticosus, and Sepharose as an active ingredient.

It is still another object of the present invention to provide a food composition for prevention or alleviation of hair loss, which comprises, as an active ingredient, a mixed extract of white porcine,

In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing or treating hair loss, which comprises a mixed extract of Phellinus linteus, Acanthopanax senticosus, and Sepharose as an active ingredient.

In addition, the present invention provides a composition for preventing or alleviating hair loss, comprising an extract of Phellodendron, Pseudomonas oryzae as an active ingredient.

In addition, the present invention provides a cosmetic composition for prevention or improvement of hair loss, which comprises, as an active ingredient, a mixed extract of Paeonia japonica, Pseudomonas sp.

In addition, the present invention provides a food composition for prevention or alleviation of hair loss, which comprises a mixed extract of Phellinus linteus, Pseudomonas sp.

The mixed extracts of Phellodendron, Spodoptera litura, and Sepharose according to the present invention are biostable and have excellent hair growth promoting effect, and thus can be usefully used as a composition for preventing or treating hair loss.

FIG. 1 is a graph showing the effect of accelerating hair growth in an animal model upon application of a mixed extract of Pigment, Spodoptera litura, and Seichoensis according to the present invention (Sample 1).
FIG. 2 is a graph showing changes in body weight during the experiment during application of the mixed extract of Pigment, Pseudomonas sp.
FIG. 3 is a diagram showing the results of dermatological analysis at the time of applying the mixed extract of Pigment, Spodoptera litura and Seicho (sample 1) according to the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention provides a composition for preventing or treating hair loss, which comprises a mixed extract of white porcine, caustic, and citric acid at a weight ratio of 100: 15 to 25: 7 to 17 as an active ingredient.

The composition includes a pharmaceutical composition, an external preparation for skin, a cosmetic composition or a food composition.

Mixed extracts of porcine, acanthopanax, and citric acid according to the present invention can be obtained as follows. Wash white porcelain, acanthan gum, and saplings with water to remove foreign matter, then dry and crush in the shade. White porcine, acanthopanax senticosus, and chicago can be used without restrictions such as cultivated or marketed. At this time, the white porcelain is preferably a fruit of a modified species of Thuja orientalis L., and it is preferable to use the leaf and stalks as a scarlet. The powdered ground white powder, viscous ground powder, and the powder of Seiho are mixed, and then an appropriate amount of solvent is added to allow complete immersion. The solvent may be selected from water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably ethanol. The extract may be impregnated or warmed at room temperature. The extract is filtered and concentrated to give a mixed extract of the final white porcelain, visa aqua and chihuahua.

In the composition of the present invention, it is preferable that the blending ratio of the white porcelain, visa aqua, and citric acid is 100: 15 to 25: 7 to 17 by weight. When the mixing ratio is out of the range, there is no significant difference in the effect of accelerating hair growth as compared with the case of using white porcelain, visa aqua, and chrysanthemum extract alone, and there is a risk of side effects.

The mixed extracts of Phellodendron, Spodoptera litura, and Sepharose according to the present invention are biostable and have excellent hair growth promoting effect, so they can be usefully used for preventing or treating hair loss.

Alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, traumatic, Trichotillomania, Pressure Alopecia, and the like can be used as the hair loss in accordance with the form, symptom or cause. But are not limited to, alopecia alopecia, non-rigid, alopecia syphiltiac, Alopecia seborrhecia, symptomatic, non-scarring, scarring and congenital alopecia.

The composition of the present invention may contain at least one known active ingredient having a hair loss preventive or therapeutic effect, together with a mixed extract of Phellodendron, Pseudomonas oryzae.

The compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. In addition, it can be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, oral preparations such as syrups and aerosols, external preparations, suppositories and sterilized injection solutions according to a conventional method. Suitable formulations known in the art are preferably those as disclosed in Remington ' s Pharmaceutical Science, recently, Mack Publishing Company, Easton PA. Examples of carriers, excipients and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose , Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner. The pharmaceutical composition of the present invention may be administered through various routes including oral or parenteral routes, but may be applied by parenteral administration, more preferably topical application by application.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and body weight of the individual, the degree of disease, the drug form, the route of administration and the period of time, and can be appropriately selected by those skilled in the art. For a desired effect, the composition of the present invention may be administered in an amount of 1 mg / kg to 10,000 mg / kg per day. The composition may be administered once a day, or divided into several doses.

The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers for hair loss prevention or treatment.

In addition, the composition of the present invention can be used as an external preparation for skin, a cosmetic composition and a food composition for prevention or improvement of hair loss.

In the case of using the mixed extract of white porcine, obtusa and chrysanthemum according to the present invention as a composition for external application for skin preventing or improving hair loss, it is further possible to use a composition containing a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, Surfactants, water, ionic or non-ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic Or lipophilic active agents, lipid vesicles, or any other ingredient conventionally used in dermatological applications. The components may also be introduced in amounts conventionally used in the related art. The external preparation for skin may be any one selected from the group consisting of cream, lotion, ointment, gel, mucilage, spray and wetting agent, but is not limited thereto.

In the case of using the mixed extract of white porcelain, acanthopanthops or acacia of the present invention as a cosmetic composition for prevention or improvement of hair loss, the mixed extract of white porcine, acanthopanax senticosus and safflower is added in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition , And may be added in an amount of preferably 0.005 to 10.0% by weight. If the content is less than 0.001% by weight, it is difficult to expect a hair growth promoting effect. If the content is more than 30% by weight, the manufacturing cost may be too high compared to the cosmetic effect.

The formulation of the cosmetic composition of the present invention is not particularly limited and can be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention may be used as a hair tonic, a hair cream, a hair lotion, a hair shampoo, a hair conditioner, a hair conditioner, a hair spray, a hair aerosol, a pomade, a powder, a gel, It can be manufactured in various forms such as solutions, sols gel, emulsion, oil, wax, aerosol such as hair growth agent, eyelash nutrition agent, pet shampoo and pet rinse, Can be used.

The composition may contain various components to be blended in a composition for preventing or improving hair loss, in accordance with the above-described various formulations or for a final purpose. For example, in the case of having a formulation of shampoo, it may contain at least one of a synthetic surfactant, which is a cleaning component, and at least one of preservatives, thickeners, viscosity regulators, pH adjusting agents, perfumes, dyes, hair conditioning agents and water. Examples of the synthetic anionic surfactant in the synthetic surfactant include alkyl and alkyl ether sulfates. Representative examples thereof include sodium lauryl sulfate, ammonium lauryl sulfate, triethanolamine lauryl sulfate, sodium polyoxyethylene lauryl sulfate, polyoxyethylene lauryl sulfate and the like . The synthetic amphoteric surfactants in the synthetic surfactants are alkyl betamines and alkylamidopropyl betaines. Representative examples include cocodimethylcarboxymethylbetaine, lauryldimethylcarboxymethylbetaine, lauryldimethyl alpha-carboxyethylbetaine , Cetyl dimethyl carboxymethyl betaine, and cocamidopropyl betaine. Examples of the nonionic surfactant include alkanolamides and amine oxides such as lauryldiethylamine oxide, palm oil alkyldimethylamine oxide, lauric acid diethanolamide, palm oil fatty acid diethanolamide, palm oil fatty acid monoethanolamide, and the like.

The cosmetic composition of the present invention may further contain at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, foaming agents, fragrances, surfactants, Or other cosmetically acceptable adjuvants, such as ionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. And, the above ingredients can be introduced in amounts commonly used in the field of dermatology.

In the present invention, the health functional food refers to a food having a biological control function such as prevention or improvement of disease, bio-defense, immunity, recovery after disease, suppression of aging, etc., and should be harmless to human body when taken over a long period of time. The mixed extracts of porcine porcupine, acanthopanax senticosus and citric acid of the present invention can be added to health functional foods for the purpose of preventing or improving hair loss. When the mixed extracts of porcine porcupine, white crocus and chrysanthemum of the present invention are used as food additives, the mixed extracts of white porcine, obtusilol and Seicho can be directly added or used together with other food or food ingredients, Lt; / RTI > The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of foods or beverages, the mixed extracts of white porphyritis, acanthopanthopsis and citric acid of the present invention are added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food. Examples of the foods to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharine and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments and production examples are provided to facilitate understanding of the present invention. However, the following Examples and Preparation Examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the Examples and Production Examples.

Example 1: Preparation of mixed ethanol extract of white porcelain, caustic glaze and citron

100 g of white porcelain, 15 ~ 25g of cauliflower and 7 ~ 17g of seaweed were respectively washed with water to remove foreign matter, followed by drying and grinding in the shade. The white porcelain was used for the modified breed, and the leaves and the stem were used for the barnyard grass. 15 g of the mixed powder was placed in 500 ml of 80% (v / v) ethanol (Merck Co., Germany), and then stirred at room temperature for 48 hours. At this time, ultrasonication was performed using an ultrasonic generator (Branson, USA) for 60 minutes three times, and the ultrasonic treatment was maintained at 40 캜 or lower. The mixture was centrifuged at 5000 rpm for 10 minutes, and the supernatant was then filtered through a 0.45 μm membrane filter (Millex-HP, Millipore, USA). The filtrate was lyophilized in a lyophilizer (Ilshin, Korea) to obtain a mixed ethanol extract of the final white porphyse, Acanthopanax senticosus and Sepharose.

Experimental Example 1. Estimation of Hair Growth Promoting Effectiveness of Mixed Extracts of White Piglets, Asteraceae and Seicho

Animal experiments were carried out in order to confirm the hair growth promoting effect of the mixed extracts of Phellodendron, Spodoptera litura and Seicho obtained in Example 1 above. The experimental animals were purchased from BioLink, a 6-week-old male C57BL / 6 mouse, and adapted to the animal breeding environment for one week. The rearing conditions were temperature 23 ± 3 ℃ and relative humidity 50 ± 10%. Experimental animals were allowed free access to water and feed. Five rabbits were used for the experiment.

First, the hair of the back part of the mouse was removed using an epilator (Product No. ER143, Panasonic Corporation, Japan), and hair was immediately removed using hair removal cream (Veet, Reckitt Benckiser, France). After 1 day, 100 쨉 l of the sample was applied to the epilation area once a day for each group. In the experimental group, mixed extracts (0.1, 0.3, 1, 3%) of white porcine, caissiflavia, and seokho were used. Only 80% ethanol was used for the negative control group and minoxidil 3% (minoxil, Hyundai Pharm) was used for the positive control group . At the 0, 7, 10, 14, 18, and 21 days after the start of the experiment, hair growth was confirmed and body weight was measured. The degree of hair growth was visually observed and evaluated as follows. If the degree of hair growth is 0%, it is 0; if it is less than 20%, it is 1; if it is less than 20% and 40% The results of confirming the hair growth promoting effect are shown in Fig. 1 and Table 1, and the weight change is shown in Fig.

Experiment Days 80% ethanol Mixed extracts of white porcine, cauliflower and chihuahua 3% minoxidil 0.1% 0.3% One% 3% 0 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0 0 ± 0.0 7 0 ± 0.0 0.2 ± 0.5 0 ± 0.0 0 ± 0.0 0 ± 0.0 0.4 ± 0.6 10 0.2 ± 0.5 1.4 ± 0.9 0.6 0.6 0.6 0.6 0.6 0.6 1.4 ± 0.8 14 1.0 ± 1.2 2.6 ± 0.9 2 ± 0.7 2.2 ± 0.8 2.4 ± 0.6 3.2 ± 1.1 18 2.6 ± 1.7 4.6 ± 0.6 4.2 ± 0.8 4.0 ± 1.0 4.2 ± 0.5 4.8 ± 0.5 21 4.0 ± 1.0 5.0 ± 0.0 5.0 ± 0.0 5.0 ± 0.0 5.0 ± 0.0 5.0 ± 0.0

As shown in FIG. 1 and Table 1, it was confirmed that the mixed extract of Phellodendron ganoderma, Acanthopanax senticosus, and Sepharose of the present invention showed an excellent hair growth promoting effect similar to that of 3% minoxidil even at a low concentration of 0.1%.

In addition, as shown in FIG. 2, there was no significant difference in body weight between the groups during the experiment. Thus, it was confirmed that the mixed extracts of white porcine, acanthopanax senticosus, and chrysanthemums of the present invention were free from biotoxicity.

Experimental Example 2. Dermal Histological Analysis of Hair Growth Promoting Effect of Mixed Extracts of White Piglets, Pseudomonas sp.

In order to confirm the hair growth promoting effect of the mixed extracts of white porcine, cauliflower and citrus of the present invention through dermal histological analysis, the following experiment was conducted. First, the skin tissues were removed from the sample treated area of the mice after 14 days of experiment, and immediately put in 10% neutral formalin and fixed at room temperature for 24 hours. After 12 hours of treatment in an autoclave, a paraffin block was prepared, and the tissue was cut into 4 mu m thick sections and stained according to hematoxylin & eosin (H & E) staining conditions. This was observed at 100 times using an optical microscope. The results are shown in Fig.

As shown in FIG. 3, it was confirmed that the mixed extracts of Phellodendron, Spodoptera litura and Sepharum of the present invention increased the number of hair follicles and the thickness of the dermis at a low concentration of 0.1%, and showed excellent hair growth promoting effect.

Experimental Example 3. Antioxidative Effectiveness of Mixed Extracts of White Piglets, Pseudomonas sp.

3-1. Free radical scavenging effect (DPPH method)

In order to measure the free radical scavenging effect of the mixed extracts of porcine porcupine, acanthopanthorrhizaea and chrysanthemum of the present invention, a known method (Mensor LL, Menezes FS, Leita GG, Reis AS, dos Santos TC, Coube CS, Leitao SG, Res., 15 (2), pp. 127-130, 2001) was slightly modified to carry out the following experiment. First, a sample solution according to the concentration of the mixed extract (0-400 占 퐂 / ml) of white porphyritis, acanthopanthopsis acantholite and citric acid according to the present invention and a solution of 0.3 mM DPPH (2,2- diphenyl-1-picrylhydrazyl, Aldrich, USA) . 750 μl of the sample solution and 300 μl of a 0.3 mM DPPH solution were mixed and allowed to stand for 30 minutes at room temperature. The absorbance was measured at 517 nm using a spectrophotometer (UV-1800, Shimadzu Corp., Japan). The free radical scavenging ability (%) was calculated by the following equation (1). L-ascorbic acid (Sigma, USA) and beta-carotene (Sigma, USA) were used as a control.

The results are shown in Table 2.

[Equation 1]

Free radical scavenging ability (%) = [1- (A / B)] 100

(A: Absorbance of each sample, B: Absorbance of the control (no sample added).)

sample
(占 퐂 / ml) 10 25 50 100 200 400 (70% ethanol) mixture of white porcelain, 2.4 ± 1.2 10.1 ± 2.3 20.2 ± 2.9 46.0 + - 2.8 82.1 ± 2.1 94.1 ± 0.6 (100% methanol) mixture of white porcelain, 6.6 ± 0.7 11.2 ± 2.8 25.3 ± 4.2 48.0 ± 5.2 81.1 ± 2.9 94.5 ± 0.6 L-ascorbic acid 95.4 ± 0.9 95.6 ± 1.1 95.7 ± 0.9 95.4 ± 1.0 95.8 ± 0.6 96.0 ± 0.4 Beta-carotene 10.3 ± 4.6 21.7 ± 4.8 30.5 ± 4.6 46.4 + - 10.0 59.9 ± 10.4 86.5 ± 8.9

As shown in Table 2 above, it was confirmed that the mixed extracts of white porphyrin, acanthopanthops, and citric acid of the present invention had excellent free radical scavenging ability similar to that of beta-carotene.

3-2. Effect of active oxygen scavenging (NBT method)

In order to measure the active oxygen scavenging effect of the mixed extracts of white porcine, obtusa, and Shiho of the present invention, a known method (Aruoma OI, Murcia A, Butler J, Halliwell B, Evaluation of the antioxidant and proxidant actions of gallic acid and its derivatives. J. Agri. Food Chem., 41 (11), pp. 1880-1885, 1993). First, 100 μl of 0.1 M phosphate buffer (pH 7.8), 100 μl of 10 mM xanthine (Sigma, USA) solution and 100 μl of 5 mM NBT (nitroblue tetrazolium, Sigma, USA) 10 [mu] l of the mixed extract of white porphyritis, Acanthopanax senticosus and Sepharose of the present invention were mixed. 100 μl of 0.3 units / ml xanthine oxidase (Sigma, USA) solution was added to each well, and the mixture was allowed to stand at room temperature for 10 minutes. Then, using a spectrophotometer (UV-1800, Shimadzu Corp., Japan) Absorbance was measured. At this time, phosphoric acid buffer was used instead of xanthine oxidase to obtain color correction values for each. The active oxygen scavenging ability (%) was calculated by the following equation (2). L-ascorbic acid (Sigma, USA) and beta-carotene (Sigma, USA) were used as a control.

The results are shown in Table 3.

&Quot; (2) "

Active oxygen scavenging ability (%) = [1- (A / B)] 100

(A: Absorbance of each sample, B: Absorbance of the control (no sample added).)

sample
(占 퐂 / ml) 10 25 50 100 200 400 (70% ethanol) mixture of white porcelain, 7.5 ± 2.1 14.5 ± 1.6 14.7 ± 1.4 21.7 ± 0.3 29.5 ± 2.4 35.6 ± 3.4 (100% methanol) mixture of white porcelain, 17.4 ± 0.8 19.3 ± 1.6 28.5 ± 2.5 47.0 ± 2.4 58.5 ± 3.5 86.7 ± 4.9 L-ascorbic acid 6.5 ± 1.8 14.1 ± 1.2 22.0 ± 1.3 58.1 ± 0.3 90.4 ± 2.1 97.0 ± 0.6 Beta-carotene 3.8 ± 1.5 7.1 ± 0.4 13.7 ± 2.6 16.4 ± 4.0 29.2 ± 5.1 43.0 ± 5.5

As shown in the above Table 3, it was confirmed that the mixed extract of Pigment, Pseudomonas oryzae of the present invention has excellent active oxygen scavenging ability.

Through the above experimental results, it was confirmed that the mixed extracts of Phellinus linteus, Pseudomonas sylvestris and Pseudomonas sp. According to the present invention have biostability and excellent hair growth promoting effect, and thus can be effectively used as a composition for preventing or treating hair loss.

Hereinafter, formulation examples of the composition of the present invention will be described, but the present invention is not intended to limit the scope of the present invention, but rather is to be specifically described.

Formulation Example 1. Preparation of a pharmaceutical composition

1-1. Manufacture of Powder

20 mg of mixed extract of white porcine, cauliflower and psyllium

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

Mixed extract of Cauliflower, Prunus persica and Sepharose 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

1-3. Injection preparation

Mixed extract of Cauliflower, Prunus persica and Sepharose 10 mg

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ .2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

Preparation Example 2. Preparation of composition for external application for skin

2-1. Manufacture of cream

7.0% by weight of a mixed extract of white porcelain,

3.0% by weight of cetostearyl alcohol,

0.5% by weight of self-emulsifiable monostearic acid

1.5% by weight of pro-type monostearic acid

0.5 wt% of beeswax, 8.0 wt% of liquid paraffin,

Squalane 7.0 wt%

4.0% by weight of isocetyl octanoate

Purified Jojoba oil 4.0 wt%

0.3% by weight of dimethylsiloxane

1.0% by weight of monostearic acid sorbitan

1.2 wt% of polyethylene glycol monostearate

Glycerin 6.0 wt%

4.0% by weight of propylene glycol

Betaine 4.0 wt%

Xanthan gum 0.06 wt%

0.10 wt% triethanolamine

Coloring matter, fragrance, preservative proper amount

Distilled water balance

2-2. Manufacture of gel

1.0% by weight of a mixed extract of white porcelain,

Glycerin 4.0 wt%

4.0% by weight of propylene glycol,

10% by weight of ethanol

0.1% by weight polyoxyethylene hardened castor oil

Carboxy polymer 0.30 wt%

0.30 wt% triethanolamine

Coloring matter, fragrance, preservative proper amount

Distilled water balance

Formulation Example 3. Preparation of cosmetic composition

3-1. Manufacture of shampoo

1.0 part by weight of a mixed extract of white porcine,

Silicone oil (100,000 cst) 1.5 parts by weight

Silicone gum 1.0 part by weight

Ammonium lauryl sulfate 12.0 parts by weight

4.0 parts by weight of ammonium polyoxyethylene lauryl sulfate

3.0 parts by weight of cocamide diether

Glycol stearate 1.0 part by weight

Laura mydropyl betaine 3.0 parts by weight

3.0 parts by weight of lauroamphocarboxy glycinate

Coloring matter, fragrance, preservative proper amount

Distilled water balance

3-2. Manufacture of soap

3.0 parts by weight of a mixed extract of white porcine,

Zinc pyrithione 3.0 parts by weight

Glycerin 1.0 part by weight

Polyethylene glycol 1.0 part by weight

Tocopheryl acetate 1.5 parts by weight

Titanium oxide 0.1 part by weight

Cocamidopropyl betaine 6 parts by weight

Purified water 4 parts by weight

3-3. Manufacture of lotions

5.0 wt% of mixed extract of white porcelain,

0.8% by weight of cetostearyl alcohol

1.0% by weight of self-emulsifiable monostearic acid

Wax 0.5 wt%

Stearic acid 0.5 wt%

Liquid paraffin 7.0 wt%

Squalane 5.0 wt%

Macadamia oil 3.0 wt%

2.0% by weight of isocetyl octanoate

0.3% by weight of dimethylsiloxane

0.5% by weight of monostearic acid sorbitan

1.2 wt% of polyethylene glycol monostearate

Glycerin 4.0 wt%

4.0% by weight of propylene glycol

Betaine 4.0 wt%

0.12% by weight of carboxy poly moiety

0.15 wt% triethanolamine

Coloring matter, fragrance, preservative proper amount

Distilled water balance

Formulation Example 4. Preparation of food composition

4-1. Manufacture of health food

100 mg of a mixed extract of white porcine, cauliflower and psyllium

Vitamin mixture quantity

Vitamin A acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

Biotin 10 μg

Nicotinic acid amide 1.7 mg

Folic acid 50 μg

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

4-2. Manufacture of health drinks

100 mg of a mixed extract of white porcine, cauliflower and psyllium

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

3.5 g of zinc oxide

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3 g

Water quantity

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 liter container, It is used in the production of the health beverage composition of the invention.

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (8)

A composition for preventing or treating hair loss, comprising as an active ingredient, a mixed extract obtained by mixing white porcine, cauliflower and sesame oil at a weight ratio of 100: 15 to 25: 7-17.
[Claim 2] The method according to claim 1, wherein the mixed extract is obtained by extracting a mixture of white powder, visa-free powder and seed powder with at least one solvent selected from the group consisting of water, an alcohol having 1 to 4 carbon atoms and a mixed solvent thereof , A pharmaceutical composition for preventing or treating hair loss.
The pharmaceutical composition for preventing or treating hair loss according to claim 2, wherein the alcohol having 1 to 4 carbon atoms is ethanol.
The method according to claim 1, wherein the hair loss is selected from the group consisting of Alopecia Areata, Androgenetic Alopecia, Telogen Effluvium, Trachomatis, Trichotillomania or Pressure Alopecia. Characterized in that it is at least one selected from the group consisting of alopecia, alopecia alopecia, alopecia syphiltiac alopecia, Alopecia seborrhecia, symptomatic alopecia, non-scarring alopecia, cicatric alopecia and congenital alopecia A pharmaceutical composition for preventing or treating.
A composition for preventing or alleviating hair loss, which comprises a mixed extract obtained by mixing white porcine, acaricide and sesame at a weight ratio of 100: 15 to 25: 7 to 17 as an active ingredient.
A cosmetic composition for prevention or improvement of hair loss comprising a mixed extract obtained by mixing white porcine, viscous olive oil and sesame oil at a weight ratio of 100: 15 to 25: 7 to 17 as an active ingredient.
The cosmetic composition according to claim 6, wherein the cosmetic composition is at least one selected from the group consisting of hair tonic, hair cream, hair lotion, hair shampoo, hair conditioner, hair conditioner, hair spray, hair aerosol, pomade, powder, gel, hair pack, hair treatment, Wherein the composition is at least one selected from the group consisting of hair growth promoting agents, hair growth promoting agents, hair growth promoting agents, hair growth promoting agents, hair growth promoting agents, hair growth promoting agents, hair growth promoting agents and eyelash nourishing agents.
Wherein the active ingredient is a mixed extract obtained by mixing white porcine, cauliflower, and sesame at a weight ratio of 100: 15 to 25: 7-17.

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