KR102467403B1 - Composition for preventing hair loss and promoting hair growth and method for manufacturing thereof - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, and a manufacturing method thereof. According to the present invention, the composition contains, as an active ingredient, a complex extract of Camellia japonica flowers, Acorus gramineus, Dictamnus dasycarpus root bark, Pleuropterus multiflorus TURCZ., Prunella vulgaris, Angelica gigas, and Chrysanthemum indicum. Therefore, the present invention may provide the composition for preventing hair loss and promoting hair growth which is harmless to the human body, can activate the scalp tissue to prevent hair loss and promote hair growth, and furthermore, can nourish the growing hair to become thicker, and the manufacturing method of the composition.

Description

Cosmetic composition for preventing hair loss and promoting hair growth and method for manufacturing the same {Composition for preventing hair loss and promoting hair growth and method for manufacturing thereof}

The present invention relates to a cosmetic composition for preventing hair loss and promoting hair growth and a method for preparing the same.

It is a reality that the hair loss population continues to increase in all age groups due to various causes such as environmental factors and social factors according to social development, and hair loss is increasing as the age group gradually decreases regardless of gender. Hair loss symptoms not only give severe stress to anyone, but also cause loss of external and internal self-confidence. Hair loss is preferably diagnosed at an early stage and prevented or treated. In particular, when the cause of genetic hair loss and the cause of acquired hair loss are combined, the symptoms of hair loss appear more severe, so it is good to diagnose and prevent it at an early stage.

Hair is formed in the hair follicle, and the hair follicle is composed of inner root sheath, outer root sheath, hair shaft, hair matrix, etc., and dermal papilla cell ) is directly related to the ability to form hair follicles and plays a key role in the formation of hair follicles, so hair follicles cannot be formed if there are no dermal papilla cells or if they do not function properly.

Common causes of hair loss include genetics, aging, excessive stress, DHT (dihydrotestosterone) production due to oversecretion of male hormones, endocrine system diseases, drug use, sensitive scalp, seborrheic scalp, and synthetic surfactants used almost every day. surfactant), perm, dyeing, etc. In addition, environmental pollution, improper eating habits and Westernization of eating habits, drinking and smoking, lack of sleep, nutritional imbalance, and excessive diet are also cited as causes of hair loss.

Currently, the prevention and treatment of alopecia is divided into a medical approach and an oriental medicine approach. Medical approaches include drug therapy, surgical methods, nutritional therapy, and dietary therapy. Drugs that promote hair growth include minoxidil (MXD) and finasteride approved by the Food and Drug Administration (FDA). However, it has been reported that minoxidil and finasteride have a problem in that hair loss proceeds normally when treatment is stopped, and side effects such as dermatitis occur when used for a long time.

Therefore, there is a need for research on a composition for preventing, improving and treating hair loss using a natural product having no side effects and high stability, and a composition for promoting hair growth by overcoming the problems of the prevention and treatment of hair loss as described above.

Conventionally, camellia flower and seokchangpo have been studied as raw materials such as cosmetics for preventing hair loss due to their scalp improvement activity.

The present inventors have conducted research on natural products that are non-toxic and have an excellent scalp condition improvement effect, and when using camellia flower and calamus as a complex extract of ringworm, sewage, Lamiaceae, angelica and chrysanthemum, the hair loss prevention effect is very good. Rather, the present invention was completed by discovering the fact that it exhibits the effect of promoting hair growth and the effect of increasing the thickness of hair.

KR 10-2065395 B KR 10-1841603 B

An object of the present invention is to provide a cosmetic composition excellent in preventing hair loss and promoting hair growth, and a method for preparing the same.

In order to achieve the above object, the present invention provides a cosmetic composition for preventing hair loss and stimulating hair growth, comprising complex extracts of camellia flower, rhododendron chrysanthemum, baekseonpi, sewage, Lamiaceae, angelica and gamguk as an active ingredient.

In one embodiment of the present invention, the composite extract may be a hot water extract.

In one embodiment of the present invention, camellia, rhododendron, baekseonpi, sewage, honey grass, angelica and gamguk are 10: 5-20: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0 may be the weight ratio of

In one embodiment of the present invention, the composite extract may further include a hot water extract of a mixture including cnidium, mugwort leaf, peppermint, medicinal buckwheat, triticale, corkscrew fruit, duckweed and nettle.

In one embodiment of the present invention, the mixture may further include vitamin tree fruit, red ginseng, purslane and cacao.

In one embodiment of the present invention, the cosmetic composition may be a formulation selected from shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist and soap.

In addition, the present invention (1) when the first heating temperature between 100 and 120 ° C. is reached by putting camellia flower, rhododendron, baekseonpi, sewage, honey grass, angelica and gamguk in water, and heating is stopped when the first heating temperature between 100 and 120 ° C is reached, then Obtaining a first extract by extracting by natural cooling; (2) Cnidium, wormwood leaves, peppermint, medicinal wheat, sambaekcho, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane, and cacao are put in water and heated to reach a second heating temperature between 70 and 90 ° C. 2 obtaining a second extract by extraction while maintaining a heating temperature; (3) When the first extract from which solids are removed in step (1) and the second extract containing solids in step (2) are mixed and heated to reach a third heating temperature between 70 and 90 ° C, the third Obtaining a third extract by extraction while maintaining the heating temperature; And (4) removing solids from the third extract, cooling it naturally to room temperature, adding a pH adjuster to adjust the pH to 5 to 7; providing a method for producing a cosmetic composition for preventing hair loss and promoting hair growth, including do.

According to the present invention, it is possible to prevent hair loss and promote hair growth by activating the scalp tissue without harm to the human body by using complex extracts of camellia flower, rhododendron, white lily, sewage, Lamiaceae, angelica and gamguk as active ingredients, Furthermore, it is possible to provide a composition for preventing hair loss and promoting hair growth and a method for producing the same, which can nourish the grown hair to make the hair thick.

Terms or words used in the specification and claims of the present invention are not limited to the usual or dictionary meaning, and the inventor can properly define the concept of the term in order to explain his/her invention in the best way. Based on the principle, it should be interpreted as a meaning and concept consistent with the technical idea of the present invention.

In the present specification, "hair loss" means a phenomenon in which hair falls off from the scalp or a state in which hair is sparse or thin, and "prevention of hair loss" means preventing and suppressing the hair loss phenomenon as described above, " "Stimulating hair growth" means promoting the generation of new hair as well as allowing existing hair to grow healthily. In general, “hair growth” takes place in the growth phase, and is promoted by induction from the telogen phase to the growth phase and delay from the growth phase to the catagen phase. The hair cycle can be divided into three main phases known as anagen, catagen, and telogen. During the growth phase, hair formation occurs as hair follicles grow deep into the skin with rapid proliferation of cells. The next phenomenon is the catagen phase, which is a transitional phase in which the cessation of cell division is prominent, and in this process, hair follicles gradually degenerate and hair growth stops. In the next phase, the telogen phase, the degenerative hair follicle contains a germ with densely packed dermal papilla cells. The initiation of new anagen events in the telogen is induced by rapid cell proliferation in the embryo, expansion of the dermal papilla and synthesis of basement membrane elements.

Therefore, it is necessary to prevent hair loss by promoting or extending the anagen phase, that is, to prevent hair loss, or to induce hair regrowth, that is, to promote hair growth, and the composition according to the present invention has the effect of preventing existing hair from falling out. , an effect of improving existing hair, such as thickening, and an effect of generating new hair. Dermal papilla cells are mesoderm-derived fibroblasts located at the base of hair follicles, and are thought to play an important role in hair growth through interaction with hair matrix cells (Jahoda CA, et al., 1984; Oliver RF, et al., 1986). In addition, minoxidil, which is currently used as a hair loss treatment, is a KATP-channel opener and has been found to increase growth in NIH3T3 fibroblasts (Sanders, et al., 1996). In addition, the composition according to the present invention can induce hair growth by activating dermal papilla cells (DPCs) and fibroblasts.

In the present specification, "alopecia" may include all hair loss classified as hair loss in the art, except for scarring (semi-renewable) alopecia. For example, it may mean hair loss of one or more of circular hair loss, androgenetic hair loss, generalized hair loss, female hair loss, childhood hair loss, adolescent hair loss, drug-induced hair loss, and seborrheic hair loss accompanying scalp skin diseases such as seborrheic dermatitis or psoriasis. .

In the present specification, "hair" means body hair, and refers to a thread-like form composed of keratin protein. Specifically, it may be hair, eyebrows, eyelashes, nose hair or body hair, but is not limited thereto.

In the present specification, when a part is said to "include" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

Although the present invention has been specifically described below, the present invention is not limited thereto.

One aspect of the present invention relates to a cosmetic composition for preventing hair loss and stimulating hair growth, comprising complex extracts of camellia flower, rhododendron chrysanthemum, baekseonpi, sewage, Lamiaceae, angelica gigas and gamguk as an active ingredient.

In the present specification, "composite extract" refers to 1) a form of extracting a mixture of camellia flower, calamus, algae, sage, lama, angelica, and chrysanthemum, or 2) camellia flower extract, calamus extract, ringworm extract, hasuoh extract, It means a mixed form of Lamiaceae extract, Angelica gigas extract, and Chrysanthemum chrysanthemum extract. The composite extract is more preferably extracted after mixing each raw material as in 1) above in terms of hair loss prevention and hair growth promotion efficacy.

In the present specification, "complex extract of camellia flower" means "complex extract of camellia flower, rhododendron, baekseonpi, hasuo, Lamiaceae, angelica and gamguk".

The “ Camellia Japonica L. )” is a flower of the camellia, an evergreen small tree belonging to the Camellia family, and is also called mountain tea flower. It is a flower of an evergreen tree that grows near the mountains and villages near the coast of Ulleungdo and Daecheongdo in the southern part of Korea. It contains a large amount of coanthocyanin and anthocyanin. In addition, the camellia flower contains camellin B, quercetin, kaemferol, sexangularetin, and ρ-hydroxybenzoic acid as phenolic compounds, protocatechuic acid ( Since it contains protocatechuic acid and gallic acid, it can increase the efficiency of collagen synthesis in fibroblasts and effectively improve skin elasticity.

The seokchangpo ( Acorus gramineus SOLAND ) is a perennial plant with evergreen leaves. The rhizome is thick and hard, has many nodes, and gives out small roots to live attached to places such as rock crevices. The seokchangpo is harvested from the roots of seokchangpo in the fall, removing the beard roots and drying in the sun. The seokchangpo contains 0.5 to 0.8% of essential oil in the rhizome. Ingredients include Asaron, Palmitinsaure, and Phenol. In addition to controlling breathing and facilitating blood circulation, the seokchangpo disperses wind and has effects such as stomach, wet, and small bells. Applied diseases are used to treat symptoms of stuffy stomach, confused symptoms, forgetfulness, and epilepsy. In addition, it is used for symptoms such as indigestion, stomach pain, abdominal pain, malignant swelling, bruises, and reddened eyes. In the present invention, it is preferable to use the "rhizome" of the iris.

The ringworm refers to the root bark of ringworm ( Dictamnus dsycarpus Turez ), which is a perennial plant of the rhubarb family. The ringworm skin is obtained by digging up the roots of ringworm in the fall, washing with water, peeling and drying. Ingredients include 0.13% of alkaloids in the root, 0.02 to 0.03% of dictamnin (dictamin) in the root bark, and also skimmianin, tricornellin, choline, dictamnolactone, fraxinelone, dictamnolide, obaku There are paddy, saponin, campesterol, sitosterol, and essential oil. In oriental medicine, it is known as an important medicinal material used for restless limbs and paralysis, and is used as a parasite medicine, and is used as a fever reducer, pain reliever, wheezing agent, antispasmodic, sedative, Used as a diuretic.

The sewage ( Pleuropterus multiflorus TURCZ.) is a vine-like perennial herbaceous plant belonging to the family Verniaceae, and in oriental medicine, the tuberous root is used as a medicine, and is known to have the efficacy of tonic, gangjeong, yanghyeol, interpolation, geopung, and small species. It is known that it can be used to improve symptoms such as physical weakness, back pain, arteriosclerosis, abduction, hypertension, chronic hepatitis, tuberculous lymphadenitis, enteritis, carbuncle, and constipation. In the present invention, it is preferable to use the root of the sewage plant.

The honey grass ( Prunella vulgaris ) is a perennial herb of dicotyledonous plants that grows in mountains or fields in each region of Korea, and is also called 'eggplant herb', and the herbal name is 'Hagocho (夏枯草)'. to be. It is about 30cm tall, and the leaves are 2-5cm long and face each other in long egg-shaped or long oval-shaped lanceolates, and the stem is square and has short hairs throughout. The flowers are reddish purple, 3-8 cm long, and the flowers are gathered in layers on the stem, and the petals that come out are shaped like lips. The fruit runs yellowish brown in July-August, and the pod remains dry in autumn. In oriental medicine, Lamigrass is used as a gonorrhea, tuberculosis, anti-inflammatory, abscess and diuretic. In the present invention, it is preferable to use at least one of the flowers, leaves and stems of Lamiaceae, preferably the aerial part.

The Angelica gigas is a perennial plant belonging to the dicotyledonous plant, umbel, umbel, umbel, and its roots are used as medicine. On the other hand, Angelica is a perennial herbaceous plant belonging to the family Umbel, and is mainly cultivated for medicinal purposes in Korea, Japan, and China. Angelica gigas Nakai produced in Korea, Angelica acutiloba Kitagawa produced in Japan, and Chinese donkey produced in China (Angelica sinensis Diels) are classified according to their origin, and their components and pharmacological effects is known to be different. Angelica gigas is a warm and non-toxic herbal medicine that is effective for gynecological diseases, high blood pressure, anemia, and blood loss, and is also used as a sedative, pain reliever, and tonic. In the present invention, it is preferable to use the root of Angelica gigas.

The Gamguk ( Chrysanthemum indicum ) is a perennial plant of the Asteraceae family, and is a wild chrysanthemum widely distributed in the mountainous regions of the central and southern parts of Korea. In oriental medicine, it is used for the purpose of antipyretic, anti-inflammatory, lowering blood pressure, relieving headache, etc., and through recent studies, antibacterial, anticancer, immunomodulating, antioxidant activities, etc. are known. In the present invention, it is preferable to use the ground part of the monitoring.

In the present invention, camellia flower, calamus, baekseonpi, sewage, Lamiaceae, angelica and gamguk are individually or as a mixture with an extraction solvent at a ratio of 1: 10 to 30, preferably 1: 15 to 25, more preferably 1: 18 After mixing and heating at a weight ratio of 22 to 90 to 120 ° C, preferably 100 to 120 ° C, more preferably 105 to 115 ° C. may have been manufactured. If the extraction temperature and weight ratio are out of the above range, the active ingredients of camellia flower, calamus, whites pisces, sewage, Lamiaceae, angelica and gamguk may be extracted in small amounts in the extract.

The extraction solvent for extracting the extract is water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. Examples of the lower alcohol include 20 to 80% by volume of methanol, ethanol, butanol or propanol.

The extraction solvent is not particularly limited, but the extract extracted with water acts more preferably to prevent hair loss and promote hair growth.

In this specification, "extract" means an effective (active) ingredient separated from a natural product. At this time, the active ingredient may be extracted from the natural product using an extraction solvent. When a natural product extract is obtained using an extraction solvent, the natural product extract may be interpreted as including both active ingredients and solvents separated from natural products. In addition, the natural product extract may refer to any one of a liquid, a dry powder thereof, and a processed product formulated using the same. In addition, the natural extract may include not only a crude extract generally used in the art, but also a fraction obtained by additionally fractionating the crude extract, a filtrate through filtration, and a purified product through purification. can

The complex extracts of camellia flower, rhododendron, chinensis, sage, Lamiaceae, angelica, and gamguk of the present invention exhibit excellent scalp protection, hair loss prevention, and hair growth promoting effects. In addition, the camellia flower complex extract of the present invention is a material derived from natural products, so it is not toxic, and even when applied to the human body, it does not cause side effects, so it can be usefully used as a cosmetic composition.

In particular, in one embodiment of the present invention, the composite extracts of camellia flower, rhododendron chrysanthemum, lily of the valley, rhododendron, silage, Lamiaceae, angelica and gamguk are synergistic or cooperative between the extracts and/or the active ingredients in the extract, respectively. It was confirmed that there is a remarkably superior hair loss prevention and hair growth promoting effect compared to the single extract of the plant.

The composite extract is a weight ratio of 10: 5-20: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0 extracts of camellia flower, seokchangpo, baekseonpi, sewage, Lamigrass, donkey and gamguk. , preferably a weight ratio of 10:5-15:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5, more preferably 10:8-12:0.8-1.2:0.8-1.2: It may be included in a weight ratio of 0.8-1.2: 0.8-1.2: 0.8-1.2. When the weight ratio of each extract is within the above range, the synergistic effect of the combined use of the extracts of camellia flower, rhododendron quincea, cinnamon cinnamon, sage, Lamiaceae, angelica and chrysanthemum extract can be maximized. When the mixing ratio of the natural substance is out of the above range, the effect of preventing hair loss and promoting hair growth may be deteriorated.

The camellia flower complex extract is a fraction obtained by re-fractionating a complex extract obtained by extracting camellia flower, rhododendron spruce, baekseon pi, sewage, Lamiaceae, angelica and gamguk with water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof and re-fractionated with a second organic solvent. can be Preferably, the camellia flower complex extract may be a fraction obtained by re-fractionating the camellia flower complex extract with hexane, ethyl acetate, butanol or water.

The term "complex extract" used herein while referring to camellia flower includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of a complex extract of camellia flower. For example, the camellia flower composite extract may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the camellia complex extract of the present invention is a processed product of the camellia flower complex extract formulated to be administered to animals in a broad sense, for example, camellia flower complex extract powder, for example, camellia flower complex extract powder. meaning to include Although the experiment was conducted with the camellia flower complex extract in the present invention, it will be expected by those skilled in the art that the desired effect can be achieved even in the form of a processed product of the camellia flower complex extract.

In the present invention, the meaning of "contained as an active ingredient" means that it contains an amount sufficient to achieve the efficacy or activity of the complex extract of camellia flower, calamus, ringworm, sage, Lamiaceae, angelica and gamguk. .

In an exemplary embodiment, the complex extracts of camellia flower, calamus, chinensis, sewage, Lamiaceae, angelica donkey and gamguk may be present in the composition in any amount desirable to provide effective hair loss prevention and hair growth promoting effects. According to one aspect, the complex extract of camellia flower, rhododendron, chinensis, sewage, Lamiaceae, donkey and gamguk may be 0.0001 to 99.9% by weight, preferably 0.001 to 30% by weight, more preferably 0.001 to 10% by weight %, more preferably 0.01 to 10% by weight, more preferably 0.1 to 5% by weight. If the content of the camellia flower, rhododendron, algae, sage, lava, angelica, and chrysanthemum composite extract is less than the above range, it is difficult to expect the effect of the composite extract, and if it exceeds the above range, the effect of the increase in the content Not only is the increase in insignificant, but there is a problem that the stability of the formulation is lowered. That is, by including the mixture of the present invention in the above range in the composition, excellent hair loss prevention and hair growth promoting effects can be implemented, formulation and product stability can be achieved, and excellent effects can be exerted in terms of economical efficiency with an optimal content.

In the cosmetic composition of the present invention, the complex extract may further include a hot water extract of a mixture including cnidium, mugwort leaf, peppermint, medicinal buckwheat, triticale, cockle fruit, duckweed and nettle.

Specifically, the complex extract is a mixture containing cnidium, mugwort leaf, peppermint, medicinal wheat, sambaekcho, cockle fruit, duckweed and nettle based on 100 parts by weight of the camellia flower, rhododendron, chinensis, sewage, Lamiaceae, angelica and chrysanthemum composite extract. 10 to 100 parts by weight of the hot water extract, preferably 20 to 80 parts by weight, more preferably 30 to 70 parts by weight, and more preferably 40 to 60 parts by weight. When the hot-water extract of the mixture is included in the above weight ratio with respect to the camellia flower, rhododendron, chinensis, sphagnum, Lamiaceae, angelica and gamguk complex extract, the effect of preventing hair loss and promoting hair growth is further improved. When the content of the hot water extract of the mixture containing cnidium, mugwort leaf, peppermint, medicinal wheat, triticale, cockle fruit, duckweed and nettle for the camellia flower complex extract is less than the lower limit, the effect of adding the hot water extract of the mixture is insignificant , When the above upper limit is exceeded, there is a problem in that the effect of preventing hair loss and promoting hair growth, which is increased due to the addition of the hot water extract of the mixture, is rather low.

The hot water extract of the mixture containing cnidium, mugwort leaf, mint, medicinal wheat, triticale, cockle fruit, duckweed and nettle is not particularly limited, but includes cnidium, mugwort leaf, mint, medicinal wheat, triticale, cockroach fruit, duckweed and nettle When the mixture is put into water and heated to reach a second heating temperature between 70 and 90 ° C., it can be obtained by extraction while maintaining the second heating temperature.

The mixture for the hot water extraction is cnidium, mugwort leaf, mint, medicinal buckwheat, sambaekcho, cockle fruit, duckweed and nettle 5: 5-10: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0 : 0.5-2.0 weight ratio, preferably 5 : 5-10 : 0.5-1.5 : 0.5-1.5 : 0.5-1.5 : 0.5-1.5 : 0.5-1.5 : 0.5-1.5 weight ratio, more preferably 5 : 8- It may be mixed in a weight ratio of 10: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2. When the weight ratio of each natural product is in the above range, the synergistic effect of the combined use of cnidium, mugwort leaf, peppermint, medicinal wheat, sambaekcho, corkscrew fruit, duckweed and nettle extract can be maximized. When the mixing ratio of the natural substance is out of the above range, the effect of preventing hair loss and promoting hair growth may deteriorate.

In addition, the mixture for the hot water extraction may further include vitamin tree fruit, red ginseng, purslane and cacao. When the mixture further includes vitamin tree fruit, red ginseng, purslane, and cacao, the effect of preventing hair loss and promoting hair growth of the cosmetic composition of the present invention is further improved, and the effect of thickening the hair is further improved.

In the mixture, the vitamin tree fruit, red ginseng, purslane, and cacao are in a weight ratio of 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0, preferably 0.5-1.5: 0.5-2.0, based on 5 parts by weight of the cnidium. It may be mixed in a weight ratio of 1.5: 0.5-1.5: 0.5-1.5, more preferably 0.8-1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2.

When the mixture further includes vitamin tree fruit, red ginseng, purslane, and cacao in the above weight ratio, respectively, the effect of preventing hair loss and promoting hair growth is further improved. If the content of the vitamin tree fruit, red ginseng, purslane and cacao in the mixture is less than the lower limit, the effect of the addition of the vitamin tree fruit, red ginseng, purslane and cacao is insignificant, and if it exceeds the upper limit, the vitamin tree fruit , there is a problem that the effect of preventing hair loss and promoting hair growth, which is increased due to the addition of red ginseng, purslane, and cacao, is rather low.

According to a preferred embodiment of the present invention, the formulation of the cosmetic composition for preventing hair loss and promoting hair growth is any one selected from shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist and soap can

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener , A chelating agent, a colorant, a preservative, a flavoring agent, etc. may be appropriately mixed, but is not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the formulation of the cosmetic composition.

Another aspect of the present invention relates to a composition for external application for skin for preventing hair loss and stimulating hair growth, comprising complex extracts of camellia flower, rhododendron chrysanthemum, chinensis dermatitis, sewage, Lamiaceae, angelica donkey and gamguk as an active ingredient.

The terms "complex extract", "anti-hair loss", and "promotion of hair growth" in the present specification are as described above.

The composition for external application for skin according to the present invention may be formulated by containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example solutions, gels, solids, anhydrous pasty products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and It may be provided in the form of a non-ionic vesicular dispersion or in the form of a cream, toner, lotion, powder, ointment, spray, pack or conceal stick. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions can be prepared according to conventional methods in the art.

In addition, the composition for external application for skin according to the present invention includes a fatty substance, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, and an ionic type. or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other commonly used in cosmetics. It may contain adjuvants commonly used in the field of cosmetology or dermatology, such as ingredients. The adjuvant is introduced in an amount generally used in the field of cosmetology or dermatology.

Another aspect of the present invention relates to a health functional food composition for preventing hair loss and stimulating hair growth, comprising a complex extract of camellia flower, rhododendron spruce, whites pisces, sewage, Lamiaceae, angelica donkey and gamguk as an active ingredient.

The terms "complex extract", "anti-hair loss", and "promotion of hair growth" in the present specification are as described above.

In this specification, the term "health functional food composition" refers to a food composition prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, 'functionality' means to obtain useful effects for health purposes such as adjusting nutrients for the structure and function of the human body or physiological functions. The health functional food composition of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food composition can be prepared without limitation as long as the formulation is recognized as a health functional food. The health functional food composition of the present invention, unlike general drugs, has the advantage of using food as a raw material and has no side effects that may occur when taking drugs for a long time, and is excellent in portability, so as to protect the scalp and promote hair growth. It can be taken as a supplement.

The health functional food composition according to the present invention, in addition to the composite extracts of camellia flower, rhododendron, whites pisces, sewage, Lamiaceae, angelica and gamguk, is preferably used within the scope of not impairing the main effect aimed at by the present invention. Other ingredients that can give a synergistic effect, for example, antioxidant compounds such as vitamin C or houttuynia cordata extract, licorice extract, moth extract, rhodiola extract, golden extract, wild ginseng extract, goji berry extract, mulberry bark extract, aloe vera leaf extract, It is also possible to contain natural products such as leaf leaf extract and tangerine peel extract.

The health functional food composition formulated in the above form may be added to food as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. Examples of food include drinks, meat, sausages, bread, biscuits, rice cakes, chocolates, candies, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, alcoholic beverages, and vitamin complexes. , dairy products and milk-processed products, etc., and includes all health functional foods in the usual sense.

In addition, the health functional food composition of the present invention is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the health functional food composition of the present invention of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not so important, but may be selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the complex extract of camellia flower, calamus, whites piranha, sphagnum, Lamiaceae, angelica and gamguk.

The dose of the active ingredient of the present invention may be limited within the level of those skilled in the art, and the daily dose of the health functional food composition of the present invention may vary depending on various factors such as the age and health condition of the subject to be administered. And, based on adults, generally 1 to 1000 mg / kg, preferably 1 to 500 mg / kg, more preferably 10 to 300 mg / kg can be divided and administered 1 to 2 times a day. And, the dosage does not limit the scope of the present invention in any way.

In the health functional food composition of the present invention, the complex extracts of camellia flower, rhododendron, algae, sage, lama, angelica, and gamguk are present in an amount of 0.01 to 5.0% by dry weight, preferably 0.01 to 2.0% by weight, based on the total weight of the composition. %, more preferably 0.01 to 1.0% by weight, more preferably 0.01 to 0.05% by weight.

Another aspect of the present invention is (1) when the first heating temperature between 100 and 120 ° C. is reached by putting camellia flower, calamus, baekseonpi, sewage, Lamiaceae, angelica and gamguk in water and heating, stopping the heating , Obtaining a first extract by extracting by natural cooling to room temperature; (2) Cnidium, wormwood leaves, peppermint, medicinal wheat, sambaekcho, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane, and cacao are put in water and heated to reach a second heating temperature between 70 and 90 ° C. 2 obtaining a second extract by extraction while maintaining a heating temperature; (3) When the first extract from which solids are removed in step (1) and the second extract containing solids in step (2) are mixed and heated to reach a third heating temperature between 70 and 90 ° C, the third Obtaining a third extract by extraction while maintaining the heating temperature; And (4) removing solids from the third extract, cooling it naturally to room temperature, adding a pH adjuster to adjust the pH to 5 to 7; will be.

As a specific example, in step (1), 10: 5-20: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5- A weight ratio of 2.0, preferably a weight ratio of 10:5-15:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5, more preferably a weight ratio of 10:8-12:0.8-1.2:0.8 -1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2, and water in a weight ratio of 1: 10 to 30, preferably 1: 15 to 25, more preferably 1: 18 to 22 After mixing and heating to reach the first heating temperature of 90 to 120 ° C, preferably 100 to 120 ° C, more preferably 105 to 115 ° C, the heating is stopped, and then 3 to 10 hours to room temperature, preferably A first extract can be obtained by extraction by natural cooling for 5 to 8 hours, more preferably 6 to 7 hours.

Next, in the step (2), cnidium, mugwort leaf, mint, medicinal wheat, sambaekcho, cockroach fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane, and cacao were prepared at a ratio of 10: 5-20: 0.5-2.0: 0.5-2.0, respectively. : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 in weight ratio, preferably 10 : 5-15 : 0.5-1.5 : 0.5- A weight ratio of 1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5:0.5-1.5, more preferably 10:8-12:0.8-1.2:0.8-1.2: 0.8-1.2 : 0.8-1.2 : 0.8-1.2 : 0.8-1.2 : 0.8-1.2 : 0.8-1.2 : 0.8-1.2 A mixture with water in a weight ratio of 1: 10 to 30, preferably 1: 15 to 25 , More preferably, when mixing and heating at a weight ratio of 1: 18 to 22 to reach a second heating temperature between 70 and 90 ° C., 1 to 60 minutes, preferably 10 to 50 minutes, while maintaining the second heating temperature , more preferably by extracting for 25 to 35 minutes.

Next, in step (3), the first extract from which solids are removed in step (1) and the second extract containing solids in step (2) are mixed and heated to a third heating temperature between 70 and 90 ° C. When the temperature is reached, a third extract can be obtained by extracting for 1 to 10 minutes, preferably 3 to 8 minutes, and more preferably 5 to 6 minutes while maintaining the third heating temperature.

Next, in the step (4), after removing the solids from the third extract and cooling it naturally to room temperature, citric acid, acetic acid, ascorbic acid, gamma linolenic acid, lactobaioic acid, lactic acid, phosphoric acid, tartaric acid, triethanolamine, sodium silicate , Sodium hydroxide, sodium citrate, sodium carbonate, and sodium borate by adding one or more types of pH adjusting agent, it is possible to adjust the acidity to pH 5 to 7, which relieves irritation of the scalp. It is not limited to the type of pH adjusting agent, and preferably, natural citric acid may be used.

In particular, (i) the extraction time in step (1) is 6 to 7 hours; (ii) the extraction time of step (2) is 25 to 35 minutes; (iii) The extraction time of the step (3) is 5 to 6 minutes, and (iv) the step (1) extracts camellia, calamus, chinensis, sage, Lamiaceae, angelica and gamguk at a ratio of 10: 8-12: 0.8 -1.2: 0.8-1.2: 0.8-1.2: 0.8-1.2: mixed at a weight ratio of 0.8-1.2, (v) the cnidium, mugwort leaf, mint, medicinal wheat, saccaria, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng , purslane and cacao were mixed in a weight ratio of 5:8-12:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2:0.8-1.2 (vi) 40 to 60 parts by weight of the second extract is mixed with respect to 100 parts by weight of the first extract in step (3), and (vii) when the pH adjusting agent satisfies all conditions of natural citric acid, it is significantly It was confirmed that all of them showed excellent hair cell proliferation promoting ability and capillary expansion ability, and as a result, the hair loss improvement and hair growth effect were very excellent.

Hereinafter, the present invention will be described in more detail through examples and the like, but the scope and contents of the present invention are reduced or limited by the examples below and cannot be interpreted. In addition, based on the disclosure of the present invention including the following examples, it is clear that a person skilled in the art can easily practice the present invention for which no experimental results are specifically presented, and the patents to which such variations and modifications are attached. It goes without saying that it falls within the scope of the claims.

<Examples and Comparative Examples>

Example 1: Preparation of camellia flower complex extract

1-1: The materials were prepared by drying and pulverizing camellia flower, rhizome of rhizome of calamus, white lily, sage root, aboveground part of Lamiaceae, root of Angelica gigas, and aboveground part of chrysanthemum chrysanthemum, respectively.

1-2: The dried and then pulverized camellia flower, calamus rhizome, whites hull, hasuo root, Lamiaceae aboveground part, angelica root, and chrysanthemum aboveground part were mixed in a weight ratio of 10: 10: 1: 1: 1: 1: 1 The mixture and water were mixed at a weight ratio of 1:20, heated, and stopped heating when the temperature reached 110° C., and then extracted while naturally cooling to room temperature for 6 hours to obtain a camellia flower composite extract. The obtained composite extract was pulverized by lyophilization through filtration and concentration under reduced pressure.

Example 2

2-1: After preparing the composite extract of camellia flower in the same manner as in Example 1, solids were removed to obtain a first extract.

2-2: Materials were prepared by drying and pulverizing cnidium, wormwood leaves, mint, medicinal buckwheat, sambaekcho, cockle fruit, duckweed, and nettle, respectively.

2-3: Cnidium, mugwort leaves, mint, medicinal wheat, sagebrush, cockle fruit, duckweed and nettle prepared by pulverizing after drying in a weight ratio of 5: 10: 1: 1: 1: 1: 1: 1 A mixture and water were mixed at a weight ratio of 1:20 and heated to reach a temperature of 80 °C, followed by extraction for 30 minutes while maintaining the temperature to obtain a second extract, which is a hot water extract of the natural product mixture.

2-4: Mix 50 parts by weight of the second extract obtained in 2-3 with respect to 100 parts by weight of the first extract obtained in 2-1 and heat to reach a temperature of 80 ° C. for 5 minutes while maintaining the temperature A third extract was obtained by extraction.

2-5: After removing the solids from the third extract and naturally cooling it to room temperature, a camellia flower composite extract was prepared by adding natural citric acid to adjust the pH to 6.

Example 3

Carried out in the same manner as in Example 2, but the mixture of steps 2-3 further includes vitamin tree fruit, red ginseng, purslane, and cacao, and the vitamin tree fruit, red ginseng, purslane, and cacao are each 1 part per 5 parts by weight of cnidium. : A composite extract of camellia flower was prepared by mixing in a weight ratio of 1: 1: 1.

Example 4

Carried out in the same manner as in Example 1, except that camellia flowers, calamus rhizomes, white aphids, rhizomes roots, Lamiaceae aerial parts, angelica root, and chrysanthemum aerial parts were mixed in a weight ratio of 1: 1: 1: 1: 1: 1: 1 to produce camellia flowers. A complex extract was prepared.

Example 5

Carried out in the same manner as in Example 1, but after mixing a mixture of camellia flower, calamus rhizome, white sandpiper, rhizome root, Lamiaceae aerial parts, angelica root, and chrysanthemum aerial parts mixed with a 70% ethanol aqueous solution at a weight ratio of 1:20, and then mixing 80 Extracted for 3 hours at ° C. to prepare a composite extract of camellia flower.

Comparative Example 1: Camellia flower extract alone

It was carried out in the same manner as in Example 1, but an extract was prepared using only camellia flowers.

Comparative Example 2: Iris extract alone

Example 1 was carried out in the same way, but an extract was prepared using only seokchangpo.

Comparative Example 3: Ringworm extract alone

It was carried out in the same manner as in Example 1, but an extract was prepared using only ringworm skin.

Comparative Example 4: Sewage extract alone

It was carried out in the same manner as in Example 1, but an extract was prepared using only sewage agar.

Comparative Example 5: Lamiaceae extract alone

It was carried out in the same manner as in Example 1, but an extract was prepared using only Lamiaceae.

Comparative Example 6: Angelica gigas extract alone

An extract was prepared in the same manner as in Example 1, except using only Angelica gigas.

Comparative Example 7: Chrysanthemum extract alone

It was carried out in the same manner as in Example 1, but an extract was prepared using only chrysanthemum chrysanthemum.

Comparative Example 8: Camellia flower and Iris extract

Carried out in the same manner as in Example 1, but the extract was prepared using a mixture of camellia and seokchangpo mixed in a 1: 1 ratio.

<Test Example>

statistical processing

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). A one-way ANOVA with Duncan's post-hoc test was used to determine the difference (p <0.05) in mean values between the experimental groups. Each of the following experiments was repeated at least three times, and each experimental data was expressed as mean ± standard deviation (SD).

Test Example 1. Cell proliferation promoting effect on dermal papilla cells

Human hair follicle dermal papilla cells (HFDPC-c) were purchased from PromoCell.

The dermal papilla cells were cultured in a culture medium additive (Supplement Mix; PromoCell, Heidelberg, Germany), HFDPC-c-specific culture medium (Follicle Dermal Papilla Cell Growth Medium; PromoCell, Heidelberg, Germany) containing 100 units/mL of penicillin and 100 μg/mL of streptomycin. ) at 37°C and 5% CO ₂ conditions.

After the cultured cells were dispensed at 3.0×10 ⁴ cells/well in a 96-well plate, they were cultured for 24 hours in a 37°C and 5% CO ₂ incubator. Thereafter, the cultured cells were treated with the composition according to Examples and Comparative Examples by concentration (0.1% by weight, 0.2% by weight, 0.5% by weight, 1.0% by weight and 2.0% by weight), and then 37 ° C. and 5% CO ₂ Incubated for 24 hours in an incubator.

Proliferation of the cells was measured by the following method using the MTT assay. Each sample was washed twice with PBS, and 200 μl/well of MTT (Sigma, MO, USA) was added and reacted for 3 hours. Thereafter, the supernatant was removed, 200 μl/well of DMSO solution was added, stirred for 30 minutes to dissolve the precipitate, and absorbance at 570 nm was measured using a microplate reader (VERSAmax microplate reader, Molecular Devices, CA, USA). measured. For absorbance, the average absorbance value for each sample group was obtained, and the growth rate was compared with the absorbance value of the control group, and is shown in Table 1 below. When the cell growth rate (survival rate) was reduced to less than 70% of the sample-free group (control), it was determined to be potentially cytotoxic. As a result of the experiment, all of the samples according to Examples and Comparative Examples showed no toxicity as the cell growth rate (survival rate) was not reduced to less than 70% at concentrations of 0.1, 0.2, 0.5, 1.0 and 2.0 dry weight%.

division Treatment concentration (dry weight %) Dermal papilla cell proliferation rate (%) Control group (untreated group) - 100 Example 1 0.1 127.1±5.4 ^## 0.5 135.5±11.6 ^## 1.0 153.8±9.7 ^## 2.0 169.3±15.3 ^## Example 2 0.1 144.2±10.9 ^## 0.5 156.3±16.4 ^## 1.0 167.8±8.7 ^## 2.0 183.4±5.9 ^## Example 3 0.1 159.6±13.1 ^## 0.5 167.9±15.2 ^## 1.0 183.3±9.7 ^## 2.0 194.5±2.4 ^## Example 4 0.1 114.2±10.1 ^## 0.5 122.1±11.3 ^## 1.0 138.8±10.9 ^## 2.0 144.5±6.2 ^# Example 5 0.1 115.6±7.9 ^## 0.5 120.2±11.5 ^## 1.0 134.6±13.8 ^## 2.0 140.7±19.6 ^## Comparative Example 1 0.1 105.2±5.7 0.5 110.2±10.4 1.0 115.1±11.2 2.0 122.4±13.0 ^** Comparative Example 2 0.1 99.4±8.6 0.5 106.2±10.4 1.0 114.2±9.2 2.0 121.0±8.1 ^** Comparative Example 3 0.1 101.3±7.4 0.5 106.2±5.9 1.0 111.3±10.6 2.0 115.4±11.7 Comparative Example 4 0.1 105.6±13.6 0.5 107.8±14.9 1.0 110.2±10.4 2.0 113.6±11.1 Comparative Example 5 0.1 99.8±12.6 0.5 101.3±8.1 1.0 102.4±9.6 2.0 100.5±10.1 Comparative Example 6 0.1 102.4±11.7 0.5 104.6±12.0 1.0 103.8±14.6 2.0 102.7±16.7 Comparative Example 7 0.1 105.4±15.2 0.5 108.5±13.8 1.0 109.1±10.7 2.0 108.4±11.4 Comparative Example 8 0.1 106.3±8.2 0.5 113.4±6.1 1.0 118.5±12.9 ^** 2.0 124.5±13.1 ^**

The ** means that there is a significant difference at p <0.05 compared to the control group, and the ## means that there is a significant difference at p <0.05 compared to Comparative Examples 1 to 8.

Looking at Table 1, it can be seen that the compositions of Examples exhibit significantly higher cell proliferation promoting effects than those of Comparative Examples 1 to 8. On the other hand, in the case of Comparative Examples 3 to 7, it can be seen that there is no significant proliferation promoting effect on dermal papilla cells.

Purba et al. reported that hair growth was promoted by promoting the proliferation of human hair follicle dermal papilla cells (HFDPC) and human epidermal cells (HaCaT cells) (Purba, T. S. et al., Characterization of cell cycle arrest and terminal differentiation in a maximally proliferative human epithelial tissue: Lessons from the human hair follicle matrix. Eur. J. Cell Biol. 96, 632-641. 2017).

From the above experimental results, it was confirmed that the composition of the present invention has an effect of promoting cell proliferation of HFDPC (dermal papilla cells), and thus it is judged to exhibit an effect of promoting hair growth.

Test Example 2: Evaluation of capillary dilation effect

When the K+ channel is opened, mitogenesis is activated and vascular smooth muscle is relaxed and blood vessels are dilated. On the other hand, it is known that the proliferation of NIH3T3 fibroblasts is inhibited when the K+ channel is blocked. Therefore, it can be seen that when the growth of NIH3T3 fibroblasts is promoted by sample treatment, K+ channels are opened, and furthermore, vasodilation is promoted.

Human fibroblasts (fibroblasts, NIH-3T3) were purchased from the Korean Cell Line Bank.

The fibroblasts were cultured in a DMEM medium containing 10% CS (calf serum) at 37° C. and 5% CO ₂ conditions.

The NIH3T3 fibroblasts were seeded in a 24-well culture plate at a concentration of 3.0×10 ⁴ cells/well, and DMEM containing 10% fetal calf serum (FCS) and 2 mM L-glutamine and no antibiotics. Incubated for 24 hours in the medium. Thereafter, the culture medium was replaced with a medium containing 0.5% by weight of the compositions of Comparative Examples and Examples of the present invention, respectively, and cultured for 72 hours. After removing the medium, it was washed with PBS (no Ca ²⁺ /Mg ²⁺ ), treated with 0.5ml TE (trypsin-ethylenediamine tetraacetic acid), and cultured. DMEM containing 10% FCS was added, the cells were taken, and then centrifuged at 6000 rpm in an EP tube. After removing the supernatant and suspending the remaining cell fluid in 100 μl of PBS, the number of cells was measured using a hemocytometer. Based on this, the capillary dilation ability of Comparative Examples and Examples of the present invention was calculated and shown in Table 2 below.

division Capillary dilation ability (%) Control group (untreated group) 100 Example 1 125.0±5.7 ^## Example 2 137.5±12.2 ^## Example 3 144.6±8.9 ^## Example 4 113.6±3.7 Example 5 115.2±13.6 Comparative Example 1 110.4±8.5 Comparative Example 2 112.9±9.1 Comparative Example 3 106.5±10.1 Comparative Example 4 103.8±6.1 Comparative Example 5 101.4±13.4 Comparative Example 6 102.4±8.3 Comparative Example 7 104.5±5.7 Comparative Example 8 113.4±7.6

The ## means that there is a significant difference at p<0.05 compared to Comparative Examples 1 to 8.

Referring to Table 2, it can be seen that the compositions of Examples have a significantly higher cell proliferation promoting effect on NIH3T3 fibroblasts than Comparative Examples 1 to 8, and furthermore, a significantly higher capillary dilation effect.

[Clinical trial]

In order to confirm whether the composition of the present invention is effective in preventing hair loss, the effect of preventing hair loss was clinically tested. As a test method, only patients who satisfied all of the following criteria were enrolled as study subjects.

[Test subject conditions]

1) A male over 20 years old in good physical and mental health who is experiencing hair loss

2) Patients who will maintain the same hair shape during the study period

3) Patients who will not dye during the study period

4) Patients who will not use special hair products or hair care and manipulation during the study period

Twenty volunteers who met the above conditions were carefully selected for the experiment, and at the beginning of the experiment, the hair condition was diagnosed, and a black dot was tattooed on the part with the most hair loss so that the same part could be measured at every measurement. did While figuring out the specific situation of each individual patient, it was recorded on the evaluation sheet and tested.

Test Example 3: Effect of improving number of hairs and hair thickness

Put 100 ml (0.5% by weight) of a solution diluted with water in each of the above Examples and Comparative Examples into a spray container and spray it on the scalp of the hair loss area of a patient with alopecia twice a day (morning and evening) three times, and check the effect. did The compositions of Examples and Comparative Examples were used twice daily for about 60 days, and after use, they were prepared and used in the same manner as described above. In the control group, minoxidil external application was applied to the scalp of the hair loss site and the expected hair loss site twice a day, and the effect was confirmed. Specifically, after measuring the number of hairs per unit area (number of hairs/cm2) and hair thickness (μm) of the hair loss area using a hair measuring device (Folliscope, Lead M), the results are shown in Table 3 below. As for the number of hairs per unit area, the number of terminal hairs in the unit area was calculated after photographing the hair loss area around the marked area using a 30x lens. The thickness of the hair was measured using a 200x lens and the thickness of 5 strands around the marked area was measured and then the average value was taken.

division Increased hair thickness (Δ㎛) Increased number of hairs (number of hairs/cm2) Control group (untreated group) -0.005 One Example 1 0.023 ^## 25.4 ^## Example 2 0.035 ^## 37.6 ^## Example 3 0.041 ^## 43.1 ^## Example 4 0.012 18.6 ^## Example 5 0.013 19.4 ^## Comparative Example 1 0.010 10 Comparative Example 2 0.012 8 Comparative Example 3 0.006 3 Comparative Example 4 0.003 5 Comparative Example 5 0.001 2 Comparative Example 6 0.002 4 Comparative Example 7 0.004 6 Comparative Example 8 0.013 8

The ## means that there is a significant difference at p<0.05 compared to Comparative Examples 1 to 8.

Looking at Table 4, it can be seen that the compositions of Examples show a positive effect on both the number and thickness of hair compared to Comparative Examples.

Preparation Example and Comparative Preparation Example: Preparation of shampoo

For use in the following test examples, shampoos containing the compositions of Examples and Comparative Examples as active ingredients were prepared according to a conventional method with the compositions shown in Table 4 below. Preparation Examples 1 to 5 are shampoos prepared with the following composition using the compositions of Examples 1 to 5, respectively, and Comparative Preparation Examples 1 to 8 are shampoos prepared with the following composition using the compositions of Comparative Examples 1 to 8, respectively. .

ingredient name Preparation Examples and Comparative Preparation Examples (% by weight) Examples and Comparative Examples
(dry weight) 0.5 Ammonium lauryl sulfate 10.00 Ammonium polyoxyethylene lauryl sulfate 5.00 Cocoamidopropyl Betaine 2.00 Ethylene glycol distearate 1.50 Cocoyl monoethanolamide 0.80 Polyquaternium-10 0.20 Blue No. 1 0.0002 Yellow No. 4 0.0001 Methylparaben 0.10 Spices 0.80 citric acid 0.10 dimethicone 1.00 Purified water To 100

Test Example 4: Hair loss prevention effect test

As a method for evaluating the hair loss prevention effect, the number of hairs falling out when washing the hair was measured. The preparation examples and comparative preparation examples were compared before and after using the shampoo for 3 months. The number of hairs falling out during hair washing was recorded once a day, and the amount of hair loss was averaged on a monthly basis and shown in Table 5 below.

division before use after 1 month of use after 2 months of use After 3 months of use Control group (untreated group) 20.2 20.6 21.4 21.3 Preparation Example 1 21.5 16.5 11.7 ^## 7.5 ^## Preparation Example 2 19.7 13.2 ^## 7.5 ^## 4.2 ^## Preparation Example 3 21.0 11.0 ^## 6.5 ^## 3.1 ^## Production Example 4 20.5 18.5 15.1 ^** 12.3 ^## Preparation Example 5 20.1 18.4 15.5 ^** 12.9 ^## Comparative Preparation Example 1 21.6 19.6 18.8 16.8 Comparative Preparation Example 2 20.7 19.5 18.9 16.2 Comparative Preparation Example 3 20.5 19.6 20.2 18.6 Comparative Preparation Example 4 20.4 19.4 19.5 19.4 Comparative Preparation Example 5 20.6 20.1 19.2 18.5 Comparative Preparation Example 6 21.7 20.5 18.7 18.0 Comparative Preparation Example 7 19.6 18.9 17.6 18.2 Comparative Preparation Example 8 20.4 19.5 17.2 15.1 ^**

The ** means that there is a significant difference at p <0.05 compared to before use, and the ## means that there is a significant difference at p <0.05 compared to Comparative Examples 1 to 8.

Looking at Table 5, it can be seen that the amount of hair loss is significantly reduced as compared to the case of using the shampoo of Comparative Preparation Example as the number of months passes after using the shampoo corresponding to Preparation Example.

Test Example 5: Hair growth promoting effect

As a method for evaluating the hair growth promoting effect, the hair lengths before and after 2 months of using the shampoo of the above Preparation Examples and Comparative Preparation Examples were compared. Hair length was recorded once daily, and the increased hair length was averaged and shown in Table 6 below.

division Increased hair length after 2 months of use (△mm) Control group (untreated group) 16.4 Example 1 24.7 ^## Example 2 26.3 ^## Example 3 27.1 ^## Example 4 21.1 ^** Example 5 21.5 ^** Comparative Example 1 19.4 Comparative Example 2 18.9 Comparative Example 3 17.3 Comparative Example 4 17.2 Comparative Example 5 16.9 Comparative Example 6 18.0 Comparative Example 7 18.1 Comparative Example 8 19.8 ^**

The ** means that there is a significant difference at p <0.05 compared to before use, and the ## means that there is a significant difference at p <0.05 compared to Comparative Examples 1 to 8.

Looking at Table 6, it can be seen that the increased hair length as months pass after using the shampoo corresponding to Preparation Example significantly increased compared to the case of using the shampoo of Comparative Preparation Example.

From the above test results, it was specifically confirmed that the composition of the present invention provides effects of preventing hair loss and promoting hair growth while being safe in vivo.

Although the present invention has been described in terms of the preferred embodiments mentioned above, various modifications and variations are possible without departing from the spirit and scope of the invention. The appended claims also cover such modifications and variations as fall within the subject matter of this invention.

Claims (8)

A cosmetic composition for preventing hair loss and promoting hair growth, comprising complex extracts of camellia flower, rhododendron chrysanthemum, lily of the valley, hawthorn, honey grass, angelica and persimmon chrysanthemum as an active ingredient,
The camellia flower, seokchangpo, baekseonpi, sewage, honey grass, donkey and gamguk have a weight ratio of 10: 5-20: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0,
The complex extract further comprises a hot water extract of a mixture containing cnidium, mugwort leaf, mint, medicinal wheat, saccaria, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane and cacao,
The above cnidium, wormwood leaf, mint, medicinal wheat, sambaekcho, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane and cacao are 5: 5-10: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: A weight ratio of 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0,
The composition is based on 100 parts by weight of the complex extracts of camellia flower, seokchangpo, baekseonpi, sewage, Lamiaceae, angelica and gamguk, the cnidium, mugwort leaf, peppermint, medicinal buckwheat, sambaekcho, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng , A cosmetic composition for preventing hair loss and promoting hair growth, comprising 10 to 100 parts by weight of a hot water extract of a mixture containing purslane and cacao. According to claim 1,
The composite extract is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that the hot water extract. delete According to claim 1,
The composite extract is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that 0.001 to 10% by weight based on the total weight of the composition. delete delete According to claim 1,
The cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that the formulation selected from shampoo, rinse, treatment, hair pack, hair gel, ampoule, hair tonic, essence, hair cream, mist and soap. (1) A mixture obtained by mixing camellia flower, calamus iris, baekseon pi, sewage, honey grass, angelica and gamguk in a weight ratio of 10 : 5-20 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 : 0.5-2.0 Obtaining a first extract by putting it in water and heating it to stop heating when it reaches a first heating temperature between 100 and 120 ° C., and then extracting by natural cooling to room temperature;
(2) cnidium, wormwood leaf, mint, medicinal wheat, saccaria, cockle fruit, duckweed, nettle, vitamin tree fruit, red ginseng, purslane, and cacao 5: 5-10: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5- 2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0: 0.5-2.0 When the mixture is mixed in water and heated to reach the second heating temperature between 70 and 90 ° C. Obtaining a second extract by extraction while maintaining the second heating temperature;
(3) 10 to 100 parts by weight of the second extract containing solids in step (2) is mixed with 100 parts by weight of the first extract from which solids have been removed in step (1), and heated to a third temperature between 70 and 90 ° C. Obtaining a third extract by extraction while maintaining the third heating temperature when the heating temperature is reached; and
(4) removing solids from the third extract, cooling it naturally to room temperature, adding a pH adjuster to adjust the pH to 5 to 7; a method for producing a cosmetic composition for preventing hair loss and promoting hair growth, including.