KR102311987B1 - Cosmetic composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss and promoting hair growth and, more specifically, to a composite preparation prepared by mixing asiaticoside, Vaccinium myrtillus extract, Andrographis paniculata extract, and Viola verecunda A.Gray extract. The composite preparation is superior in the effects of inhibiting 5α-reductase activity and promoting the proliferation of human dermal papilla cells, compared with each preparation, and contains non-toxic natural raw materials as active ingredients. Accordingly, the present invention provides a cosmetic composition that can be effectively used as a hair loss prevention agent and a hair growth promoting agent.

Description

Cosmetic composition for preventing hair loss and promoting hair growth {Cosmetic composition for preventing hair loss and promoting hair growth}

The present invention relates to a method for producing a cosmetic composition for preventing hair loss, proliferating human hair papilla cells and promoting hair growth, and more particularly, it contains asiaticoside, bilberry extract, cheonsimryeon extract and soybean violet extract as active ingredients. It relates to a cosmetic composition.

Hair loss in modern people is not only caused by aging or genetic factors, but also by acquired factors such as various environmental pollution, work stress, and abnormal hormone secretion due to changes in diet. Accordingly, the hair loss market is rapidly expanding due to the increase in the number of hair loss populations in Korea, and the hair loss related industry has entered a growth phase. The hair loss market is subdivided into hair loss management products, devices, management rooms and medical procedures, and related new products are being developed one after another.

In particular, the market for cosmetics such as shampoo is the largest, but quasi-drugs are emerging one after another, and home shopping is the main sales channel. Hospitals are also expanding the market for pharmaceuticals and hair transplants, and in the case of management offices, alternative medical management is currently being performed in hospitals and beauty salons, starting from specialized management offices.

On the other hand, hair loss is physiological hair loss. Generally, hair has a growth cycle of growth (3 years)-regressive (3 weeks)-resting (3 months). It is classified as abnormal hair loss (Alopecia), in which the hair of the growing season suddenly enters the resting phase and falls out a lot due to febrile disease, pregnancy, or mental stress. Hair growth is most active between the ages of 15 and 30, and gradually regresses between the ages of 40 and 50. If you are over 50 years old, natural hair loss due to aging starts to occur, and hair loss becomes faster after the age of 70. Normally, about 50 to 100 normal catagen hair loss occurs per day. There is a need.

In general, the most typical form of hair loss is androgenetic alopecia, which occurs due to excessive male hormone activity, and is dependent on androgens, which are male hormones. Representative androgens include testosterone and DHT (dihydrotestosterone). It is known that many Testosterone is converted to DHT by 5α-reductase, and this conversion occurs by 5α-reductase of Type 2 among two types of 5α-reductase, Type 1 and Type2, and conversion It has been reported that the gradual reduction of the dermal papilla and hair follicles is progressed by DHT.

Currently, the most widely used in the treatment and prevention of such alopecia are minoxidil-containing topical application approved by the US Food and Drug Administration (FDA), and oral preparations of Finasteride and dutasteride. Among them, minoxidil is currently the only application approved for the treatment of hair loss in Korea.

Minoxidil was used as an oral antihypertensive drug in 1979, and hirsutism was observed as a side effect in clinical trials, so it was developed and used as a topically applied hair growth agent. It was approved by the FDA as a treatment for male hereditary hair loss and was sold under the trade name Rogaine. , J Korean Med Assoc 2020 May; 63(5):277-285)

However, although the exact mechanism of hair growth of minoxidil is still not clearly known, it acts on peripheral blood vessels to induce local increase in blood flow to the skin to promote hair growth, and directly acts on hair follicles to induce hair growth. As for side effects, some side effects on the cardiovascular system have been reported, but they are rare and mild. Local side effects are relatively common, and irritation reactions such as dryness, itching, and erythema of the scalp are common. Also, hirsutism, in which the hair on the forehead or cheeks thickens, is a common occurrence in women. In addition, when minoxidil is used for a long period of time, its effect is halved, and as a side effect due to chemical vasodilation, asthenia and sebum increase have been reported. (Messenger AG, Rundegren J. Minoxidil: mechanisms of action on hair growth. Br J Dermatol 2004;150:186-194)

The effect of minoxidil mainly slows down the hair loss process and makes the hair grow, but it is difficult to expect the effect of long thick hair. There are disadvantages. (Kor. J. Aesthet. Cosmetol., Vol. 12 No. 6, 773-786, December 2014)

On the other hand, recently, it has been reported that natural products exhibit antioxidant and immunomodulatory functions in various studies, and fields related to natural products are growing in quantity and quality with high reliability and increased use worldwide due to the development of the well-being industry. It is a trend that is getting more and more spotlight, and research to explore ingredients that have the effect of preventing hair loss and hair growth in herbal medicines is active. Currently, drugs that are expected to prevent hair loss and promote hair growth are mainly drugs that have tonic and regenerative effects or drugs that improve skin local blood flow. Anti-hair loss agents are expected to be put to practical use in the future.

As such, extracts made of single or mixed natural substances tend to help prevent hair loss. In this regard, Korean Patent Laid-Open Patent No. 10-2010-0011176 (Reference 1) discloses that in the composition for preventing hair loss and promoting hair growth using oriental herbal complex extract, chrysanthemum, safflower, marigold, gold and silver flower, seonbokhwa and badger oil mixed extracts are used for hair loss. Korean Patent Laid-Open Publication No. 10-2016-0093816 (Reference 2), which is effective in improving hair, contains herbal extracts for promoting wool or hair growth, and a cosmetic composition containing the same, including herbal extracts of sauerkraut, arborvitae, cucurbita, wolfberry and eoseongcho. characterized in that In addition, Korean Patent Laid-Open No. 10-2020-0091361 (Reference 3) discloses a composition for preventing hair loss or promoting hair growth comprising a mixed extract of cucumber, eggplant and snail as an active ingredient, Korean Patent Laid-Open No. 10-2020-0002923 In No. (Reference 4), there is a cosmetic composition for preventing hair loss comprising extract of woobangja as an active ingredient, and in Korean Patent Laid-Open No. 10-2018-0082054 (Reference 5), hair growth promotion containing pumpkin seeds, mung beans, walnuts and nettle extracts And a cosmetic composition for preventing hair loss has been disclosed.

However, the results of studies with these natural products show clinically clear effects, but since these natural products contain a small amount of effective active ingredient for hair growth, it is difficult to obtain sufficient effects for hair loss treatment or hair growth promotion compared to the effects verified in the laboratory stage. Uneasy.

Reference 1: Republic of Korea Patent Publication No. 10-2010-0011176 Reference 2: Republic of Korea Patent Publication No. 10-2016-0093816 Reference 3: Republic of Korea Patent Publication No. 10-2020-0091361 Reference 4: Republic of Korea Patent Publication No. 10-2020-0002923 Reference 5: Republic of Korea Patent Publication No. 10-2018-0082054

Therefore, the present invention for solving the problems of the prior art contains asiaticoside, which is well known for its collagen synthesis enhancing effect, has a good antioxidant effect, and contains an extract effective for hair loss and hair growth, thereby maintaining the hair cycle in the growth phase for a long time. An object of the present invention is to provide a cosmetic composition that prevents hair loss and promotes hair growth and has an effect of preventing hair loss and promoting hair growth without irritation to the scalp even when used for a long period of time.

In particular, an object of the present invention is to provide a cosmetic composition in which the effect of preventing hair loss and promoting hair growth is maximized compared to the efficacy of each formulation by mixing bilberry extract, cheonsimryeon extract and soybean violet extract with asiaticoside.

The present invention in order to solve such a technical problem;

It provides a cosmetic composition for preventing hair loss and promoting hair growth, comprising asiaticoside, bilberry extract, cheonsimryeon extract and soybean violet extract as active ingredients.

In this case, the asiaticoside is characterized in that it contains 0.01 to 5.0% by weight based on the total weight of the cosmetic composition.

And the natural mixture composition consisting of the bilberry extract, cheonsimnyeon extract and soybean violet extract is characterized in that it contains 1.0 to 30% by weight based on the total weight of the cosmetic composition.

In addition, it is characterized in that the bilberry extract, cheonsimryeon extract and soybean violet extract are mixed in a weight ratio of 1: 1: 2.

In addition, the cosmetic composition for preventing hair loss and promoting hair growth is selected from the group consisting of astringent lotion, softening lotion, lotion, cream, essence, pack, cleansing foam, cleansing cream, shampoo, conditioner, hair tonic, and massage cream. It is characterized by having a formulation.

The cosmetic composition for preventing hair loss and promoting hair growth according to the present invention contains Asiaticoside, Vaccinium myrtillus extract, Andrographis paniculata extract, Viola verecunda A.Gray var extract as active ingredients. It is excellent in preventing hair loss and promoting hair growth.

In addition, the cosmetic composition for preventing hair loss and promoting hair growth according to the present invention is non-toxic because it is a material derived from a natural product, and has excellent safety because it does not cause side effects even when applied to the human body.

In addition, it should be understood that the effects of the present invention are not limited to the above-described effects and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 shows the results of Comparative Examples and Examples for the proliferation-promoting proliferation rate of human hair papilla cells according to Test Example 2 of the present invention.
Figure 2a is a photograph showing the degree of hair growth in mice after applying Comparative Example 4 according to Test Example 3 of the present invention.
Figure 2b is a photograph showing the degree of hair growth in mice after application of Example 5 according to Test Example 3 of the present invention.
Figure 2c is a photograph showing the degree of hair growth in mice after application of Example 6 according to Test Example 3 of the present invention.
Figure 2d is a photograph showing the degree of hair growth in mice after application of Example 7 according to Test Example 3 of the present invention.
3 is a graph showing the results of evaluating the degree of alleviation of the hair loss symptom of Table 5 and the degree of improvement of the condition of the hair and scalp on a 5-point scale.
4 is a photograph of changes in hair of the parietal part before and 3 months after use of the participants who participated in the test evaluation of Example 7;

Hereinafter, the characteristics of the cosmetic composition for preventing hair loss and promoting hair growth according to the present invention will be understood by the embodiments described in detail with reference to the accompanying drawings.

Since the present invention can have various changes and can have various forms, embodiments will be described in detail in the text. However, this is not intended to limit the present invention to the specific disclosed form, it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention.

The cosmetic composition for preventing hair loss and promoting hair growth according to the present invention contains asiaticoside, which is well known for its collagen synthesis enhancing effect, and contains bilberry extract, cheonsimryeon extract, and soybean violet extract that have good antioxidant effect and are effective for hair loss and hair growth. By doing so, it maintains the hair cycle during the growth phase for a long time, thereby preventing hair loss and promoting hair growth, and there should be no irritation to the scalp even after long-term use.

In addition to asiaticoside, the cosmetic composition of the present invention contains bilberry extract, cheonsimryeon extract and soybean violet extract as active ingredients. In this case, the method of extracting the bilberry extract, cheonsimryeon extract and soybean violet extract is not particularly limited, and may be extracted according to a method commonly used in the art. As an example, the present invention extracts bilberry, cheonsimryeon, and soybean violet under reflux with purified water, respectively, and then freeze-dried to make a concentrate using a conventional concentrator to extract bilberry extract, cheonsimryeon extract and soybean violet extract.

Here, asiaticoside has a complex structure in which a sugar is attached to an asiatic acid in a structure in which five hexagonal rings are bonded. As a natural product contained in a large amount in Centella asiatica, asiaticoside has been widely used for skin diseases since ancient times because it protects damage caused by burns and cuts or improves the healing rate. Asiaticoside has a good therapeutic effect on scar proliferation and scar mass, and can prevent erythema on the skin due to UV irradiation. Therefore, it is a research target material that is receiving a lot of attention, such as developing asiaticoside as a functional cosmetic to prevent and treat related skin diseases. (Shim et al, 1996. 6, 2937-2940, Bioorg. Med. Chem. Lett .)

And bilberry is a deciduous broad-leaved shrub of blue-colored edible fruit called Holtleberry as European blueberry (Vaccinium myrtillus). Although they have a lot in common with the blueberry (Vaccinium cyanococcus), they are more accurately called blue whortleberry and common bilberry to distinguish them from other cherries. It grows about 15-50cm tall, and the stem is yellow and the young branches are green. Flowers bloom in May-June and come out one by one from the leaf axil. Fruits ripen in July-September are round, ultramarine, and have a maximum diameter of 2cm. The main substance that helps eye health in bilberry is anthocyanin (or anthocyanoside), which is an antioxidant that plants produce to protect themselves from UV rays or viruses. It facilitates the supply of blood and nutrients to the In addition to anthocyanins, vitamin C, vitamin E, and resveratrol (an antioxidant found in red wine) also play a role in the antioxidant capacity of bilberries. The fruit contains 3~4 times more anthocyanin and flavonoid compound than blueberry, so it is used as a healthy fruit along with blueberry in various dietary life. The leaves have long been used medicinally for diabetes, retinal protection caused by sugar, and hypertension.

Also, Andrographis paniculata (穿心蓮) is an annual plant of the family Acanthaceae and grows throughout India, especially in southern fields and forests, and is cultivated as a medicinal plant. The height is 0.3 ~ 1.0m, the stem is dark green, 2 ~ 6mm thick, and the leaves are 8cm long and 2.5cm wide in the shape of a willow leaf, and the flowers have purple spots on white or light pink petals. All parts of this plant have an extremely bitter taste, so they are known as 'Maha-tila' (king of bitters). Of the 28 plants of the genus Andrographis, only a few have a weak phytospore, of which cheonsimryeon is the best known. According to Ayurveda, this plant has bitter, bitter, and cold properties and is used as a laxative, trauma treatment, anti-inflammatory, digestive, and anthelmintic. The main ingredient is adrographolide, and there are andrographiside, andropanoside, andrographin, etc., which are contained in leaves rather than stems. In modern medicine, it is widely used in functional drinks after liver disease, jaundice, and hangover, and its anticancer effect is being studied a lot.

And Viola verecunda A.Gray var, also called soybean barracuda, champignon, and sagebrush, is a perennial plant belonging to the family Violaaceae. The height is about 20cm, and several thin stems extend almost straight from the short rhizome. The white flowers bloom in sequence from spring to early summer. The fruit is egg-shaped capsule, which is split into 3 parts when ripe. It lives mainly in fields and is distributed all over Korea. The glycoside component in violet has the effect of inhibiting the growth of cancer cells, and is also used as a growth promoter and liver function promoter.

The cosmetic composition of the present invention contains 0.01 to 5.0% by weight of asiaticoside as an active ingredient in the total weight of the cosmetic composition (based on 100% by weight) and 1.0 to 30% by weight of a natural mixed composition in order to increase the effect of preventing hair loss and promoting hair growth. include

At this time, the natural mixture composition is a mixture of bilberry extract, cheonsimryeon extract and bean fern extract, and it is preferable to mix bilberry extract, cheonsimryeon extract and soybean violet extract in a weight ratio of 1: 1: 2.

Here, when the amount of asiaticoside is less than 0.01% by weight based on the total weight of the cosmetic composition (based on 100% by weight), the effect is insignificant, and when it exceeds 5.0% by weight, the solubility is deteriorated, so the stability is not good. In addition, the natural mixture composition consisting of bilberry extract, cheonsimryeon extract and soybean violet extract has insignificant effects even when it is less than 1.0% by weight based on the total weight of the cosmetic composition (based on 100% by weight), and even if it exceeds 30% by weight, hair compared to the content The growth promoting effect is not great.

In one embodiment of the cosmetic composition according to the present invention, when a composition containing all of asiaticoside, bilberry extract, cheonsimryeon extract, and soybean violet extract is applied, significantly excellent hair growth promoting effect and 5-alpha reductase activity inhibitory effect , it was confirmed that the effects of promoting hair growth and clinical trials using mice were excellent.

The cosmetic composition of the present invention is prepared in a formulation selected from the group consisting of solutions, creams, nutrient lotions, softening lotions, packs, emulsions, essences, soaps, liquid detergents, hair tonics, hair shampoos, body washes, and hair treatments. can, but is not limited thereto.

Hereinafter, efficacy experiments of the cosmetic composition according to the present invention will be described.

<Preparation Example 1: Preparation of bilberry extract>

10 times purified water was added to 100 g of dried bilberry and extracted under reflux at 90 ~ 100℃ for 4 hours. The extract was filtered through filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60°C using a conventional concentrator (EYELA, Japan). Thereafter, the concentrate was freeze-dried to obtain 35.4 g of bilberry extract by dry weight, which was used as the following experimental sample.

<Preparation Example 2: Preparation of Cheonsimnyeon Extract>

10 times purified water was added to 100 g of dried Cheonsimnyeon and extracted under reflux at 90 ~ 100℃ for 4 hours. The extract was filtered through filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60°C using a conventional concentrator (EYELA, Japan). Then, the concentrate was freeze-dried to obtain 29.3 g of cheonsimryeon extract by dry weight, which was used as the following experimental sample.

<Preparation Example 3: Preparation of Soybean Violet Extract>

About 10 times of purified water was added to 100 g of dried bean violets and extracted under reflux at 90 ~ 100 °C for 4 hours. The extract was filtered through filter paper (Whatman, Dassel, Germany), and then concentrated under reduced pressure at 60°C using a conventional concentrator (EYELA, Japan). Thereafter, the concentrate was freeze-dried to obtain 25.9 g of bean violet extract by dry weight, which was used as the following experimental sample.

Hereinafter, with the natural extract extracted through Preparation Examples 1 to 3, mixed compositions of various mixing ratios were prepared by mixing at the mixing ratio as shown in [Table 1], respectively, and the hair growth promoting efficacy of the cosmetic composition according to the present invention was tested. explain about

Mixed weight ratio of extracts division Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 Example 4 Bilberry Extract One - - One 2 One One Cheonsimnyeon Extract - One - One One 2 One Soybean Violet Extract - - One One One One 2

# Test Example 1: Confirmation of 5-alpha-reductase activity inhibitory effect

The 5-alpha-reductase used in the 5-alpha-reductase activity inhibition experiment was obtained from rat liver (adult, Sprague-Dawley, male rat (8-10 week oil, testis)), and a certain amount of protein suspension was obtained through protein quantification. (protein auspension) was prepared.

^{Radio-labeled testosterone (3} H testosterone) was used as a substrate for 5-alpha reductase, (enzyme reaction solution: protein suspension: each extract at a mixed weight ratio of A1 to A7 = 70:20: After reacting in an incubator for 30 minutes at a ratio of 10), extraction was performed with ethyl acetate, and 100 microliters of the ethyl acetate phase was developed on a thin layer chromatogram (TLC), and the conversion rate to developed testosterone was measured. Thus, the inhibition rate of 5-alpha-reductase was evaluated by comparing the result with the control (conversion rate when no extract was added). The method of calculating the inhibition rate is as follows.

Conversion rate = amount of radioactivity in the DHT region / total amount of radioactivity

5-alpha reductase activity inhibition rate (%) = [(A-B)/A] X 100

Here, A = conversion rate from testosterone to dihydrotestosterone when no extract was added (negative control), and B = conversion rate from testosterone to dihydrotestosterone when extract was added.

division density dihydrotestosterone testosterone Total Conversion rate (%) Inhibition rate (%) (ug/ml) (dpm) (dpm) (dpm) negative control 15454 20483 35937 43.0 Comparative Example 1 One 11012 30125 41137 26.8 37.8 10 9871 32154 42025 23.5 45.4 100 7787 34564 42351 18.4 57.2 Comparative Example 2 One 11234 29784 41018 27.4 36.3 10 10124 31454 41578 24.3 43.4 100 8977 33451 42428 21.2 50.8 Comparative Example 3 One 9867 30124 39991 24.7 42.6 10 8879 31454 40333 22.0 48.8 100 7458 35467 42925 17.4 59.6 Example 1 One 9687 32516 42203 23.0 46.6 10 6784 33456 40240 16.9 60.8 100 6084 34571 40655 15.0 65.2 Example 2 One 9546 30124 39670 24.1 44.0 10 7214 34676 41890 17.2 60.0 100 5978 35647 41625 14.4 66.6 Example 3 One 9978 32146 42124 23.7 44.9 10 8014 34215 42229 19.0 55.9 100 5978 39457 45435 13.2 69.4 Example 4 One 8012 31456 39468 20.3 52.8 10 6584 36778 43362 15.2 64.7 100 5014 40326 45340 11.1 74.3

As shown in [Table 2], each composition inhibited the activity of testosterone 5-alpha-reductase in a concentration-dependent manner. It was confirmed that the inhibitory effect was the best when mixed in a ratio of 1:1:2.

<Preparation Example 4>

[Table] 3], a cosmetic composition for preventing hair loss and promoting hair growth was prepared.

division Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Example 5 Example 6 Example 7 award part Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 glycerin 5 5 5 5 5 5 5 5 5 5 propylene glycol 10 10 10 10 10 10 10 10 10 10 Disodium EDIT 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 minoxidil 5.00 - - - - - - - - - Sodium Hyaluronate 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 solubilization part ethanol 5 5 5 5 5 5 5 5 5 5 Polyglyceryl-10 Stearate One One One One One One One One One One Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 caprylyl glycol 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 additive Asiaticoside - 0.01 2.5 5 - - - 0.01 2.5 5 Example 4 - - - - One 5 10 One 5 10

The cosmetic composition of [Table 3] dissolves the aqueous part and the solubilizing part, respectively, and then puts the solubilized part in the aqueous part, stirs and mixes for 20 minutes, adds the additive to completely dissolve, and then matures in a constant temperature chamber at 30 ℃ for 24 hours After that, it was confirmed that the formulation was free from phase separation or suspension.

# Test Example 2: Proliferative effect of human hair papilla cells

Human Hair Dermal Papilla Cells (ScienceCell Research LaboratoriesCo., USA) were inoculated in a 96-well plate to 1.5 X 104 cells/well, and then 10% fetal bovine serum (Gibco Co., USA) Using this containing DMEM (Gibco Co., USA) medium, it was cultured for 24 hours at 37° C., 5% CO 2 incubator. Then, each test substance was diluted with DMEM medium without fetal bovine serum and treated for 24 hours at each concentration (0, 25, 50, 100 μg/mL), and then 5 mg/mL of MTT (3-(4) ,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution was added to a final concentration of 1 mg/mL, and incubated for an additional 4 hours. Finally, discard the supernatant, add 200 μL of DMSO (Sigma Co., USA) to each well, dissolve crystal violet in a shaking incubator (TW3, Fine PRC, Korea) for 30 minutes, and then use a microplate reader (BIO-TEK Instruments Inc.). , Power Wave X340, USA) was used to measure the absorbance at 570 nm. The effect of each test substance prepared in [Table 2] on the proliferation of human dermal papilla cells was expressed as a percentage of the absorbance of the control group not treated with each test substance. Minoxidil was used as a positive control containing 5% by weight, the standard notified by the Ministry of Food and Drug Safety.

test substance Human dermal papilla cell proliferation rate (% control) 25 μg/mL 50 μg/mL 100 μg/mL control 100 100 100 Comparative Example 4 140.5 154.8 168.7 Comparative Example 5 115.1 121.7 122.3 Comparative Example 6 119.4 122.1 132.8 Comparative Example 7 128.3 139 149.3 Comparative Example 8 109.1 112.8 119.2 Comparative Example 9 125.7 135.4 149.6 Comparative Example 10 130.4 145.2 154.6 Example 5 127.7 135.9 145.9 Example 6 135.2 155.8 164.5 Example 7 142.8 162.8 184.5

As shown in the results of [Table 4], it was confirmed that the proliferation rate of human dermal papilla cells was increased when the positive control group was treated, and the treatment concentration was also increased during the treatment of Comparative Examples 5 to 10 and Examples 5 to 7 of the present invention. It was confirmed that the proliferation of human dermal papilla cells was promoted in a dependent manner. In particular, it was confirmed that the composition of Example 7, 5% by weight of asiaticoside, and Example 4, 30% by weight, showed the same or superior effect than the composition containing 5% by weight of minoxidil of Comparative Example 4, thereby promoting the proliferation of human dermal papilla cells the most. could

# Test Example 3: Measurement of the effect of promoting hair growth using a mouse

In order to examine the effect of the asiaticoside and complex extract used in the present invention, the animal used in the test was a male C57BL/6 mouse, and was used for waxing on the 49th to 51st days after entering the second catagen. of the hairs were completely removed, and 100 μl of the compositions of Comparative Examples 4, 5, 6 and 7 were applied twice a day for 15 days, which was used for the time lapse and test, and all the animals used in the test No significant body weight changes or adverse reactions were observed. Drinking water and feed were provided sterilized, but there were no restrictions on intake. In order to check the presence or absence of abnormalities in the experimental animals, body weight was measured daily during the test period as well as the adaptation period, but no weight loss was observed.

For hair removal of C57BL/6 mice, first, hair was removed with an animal electric clipper, then a depilatory agent (Yuhan Corporation, Ner Cream) containing thioglycolic acid was applied again, and the hair was completely removed with soft cotton after 1 minute. The remaining depilatory agent was completely removed using a cotton pad soaked in water, and each sample was applied 2 days after depilation. All applications were carried out for 14 days, and only the solvent (50% ethanol, 30% butylene glycol, 20% distilled water) used for dissolving the sample was applied to the negative control group.

As shown in the test results using the C57BL/6 mouse (1) in FIGS. 2a to 2d, the hair growth promoting effect of Examples 5, 6, and 7 treatment groups was that of Comparative Example 4, a control group currently used as a hair loss related drug. Minoxidil 5% by weight treatment group showed a similar degree of hair growth promoting effect, and side effects such as weight loss or skin irritation were not observed in all of the experimental groups.

Therefore, it was confirmed that the cosmetic composition comprising asiaticoside, bilberry extract, cheonsimryeon extract, and soybean violet extract of the present invention is a safe material at the same time having excellent hair growth promoting effect.

# Test Example 4: Clinical Trial Efficacy Evaluation

15 male and 5 female subjects (M-shaped & round 43%, M-shaped 14%, M-shaped 43%) were randomly divided into 3 groups and served in Incheon area with symptoms of hair loss, aged 40 and 60 years old. Minoxidil cream sold by the company and the cosmetic compositions of Comparative Examples 4 and 7 prepared in Table 3 were used once a day for 3 months before bedtime.

After 3 months of using each of the provided products, the effects of taking pictures of the hair and scalp of all the test subjects participating in the test were measured for a total of 3 times. In order to photograph the same place every time, the photographing method was measured by capturing the characteristics of the subject's crown or central point. As a result, the degree of alleviation of hair loss symptoms and the degree of improvement in the condition of hair and scalp were evaluated on a 5-point scale, and the results are shown in [Table 5] below.

division Comparative Example 4 Example 7 minoxidil cream hair growth 2.3 4.1 4 Relieves scalp irritation 2.5 3.5 3.5 Reduce hair loss 2.1 4.6 4.2 hair thickness 2 4.0 3.9 Overall satisfaction 1.4 4.8 4.7

According to [Table 5], Example 7 and minoxidil cream showed excellent effects in hair growth and hair loss reduction items, and improved scalp inflammation and hair thickness were also excellent. Therefore, the effect of improving the overall hair loss symptoms was clear, and most of the patients who participated in the test evaluation showed satisfaction.

On the other hand, Figure 4 is a photograph of the hair change of the parietal part 2 before and after the test evaluation of the participant who participated in the test evaluation. It was confirmed that the hair was expressed and the density of the hair was increased.

Therefore, the cosmetic composition comprising asiaticoside, bilberry extract, cheonsimryeon extract, and soybean violet extract as an active ingredient has an effect of inhibiting 5-alpha-reductase activity, promoting the proliferation of human hair papilla cells, It was confirmed through several experiments that the effects of promoting hair growth using mice and clinical trials were excellent.

<Preparation Example 1: Preparation of hair tonic>

The composition of the hair tonic containing Example 4 and the cosmetic composition containing Asiatico as an active ingredient is shown in Table 6 below.

division Preparation Example 2 function Purified water To 100 solvent glycerin 2.00 moisturizer dipropylene glycol 10.00 moisturizer Disodium EDIT 0.02 sequestering agent Asiaticoside 5.00 Hair loss prevention and hair growth promoter betaine 1.00 moisturizer ethanol 5.00 astringent Polyglyceryl-10 Laurate 0.50 solubilizer Tocopheryl Acetate 0.05 antioxidant Spices 0.10 flavoring agent caprylyl glycol 0.05 moisturizer Ethylhexylglycerin 0.05 moisturizer Cosmetic composition of Example 4 30.0 Hair loss prevention and hair growth promoter

< Preparation Example 2: Shampoo >

The composition of the hair tonic containing Example 4 and the cosmetic composition containing Asiatico as an active ingredient is shown in Table 7 below.

division Preparation Example 4 function Purified water To 100 solvent Sodium Laureth Sulfate 30.00 Surfactants Sodium Lauryl Sulfate 15.00 Surfactants Lauryl Glucoside 10.00 Surfactants Sodium Cocoyl Glucoside 5.00 Surfactants Decylglucoside 2.00 Surfactants Asiaticoside 5.00 Hair loss prevention and hair growth promoter propanediol 1.00 moisturizer Poloxamer 407 0.70 thickener panthenol 0.50 skin improvement agent Polyquaternium-10 0.30 thickener hydroxyacetophenone 0.10 preservative Disodium EDIT 0.02 sequestering agent Spices 0.15 flavoring agent glycerin 0.50 moisturizer Cosmetic composition of Example 4 10.00 Hair loss prevention and hair growth promoter

<Production Example 3: Hair treatment>

The composition of the hair tonic containing Example 4 and the cosmetic composition containing Asiatico as an active ingredient is shown in Table 8 below.

division Preparation Example 4 function Purified water To 100 solvent cetyl alcohol 6.00 emulsion stabilizer dimethicone 6.00 skin conditioning agent Butylene Glycol 5.50 moisturizer Asiaticoside 5.00 Hair loss prevention and hair growth promoter Steatrimonium Chloride 2.00 antistatic agent Spices 1.50 flavoring agent propylene glycol 1.00 moisturizer Cocamidopropyl Betaine 1.00 Surfactants Guarhydroxypropyltrimonium chloride 0.50 antistatic agent Bis(C13-15 Alkoxy) Sebum-Amodimethicone 0.40 hair conditioning agent C14-15 alcohol 0.40 skin conditioning agent isotridecyl alcohol 0.30 skin conditioning agent 1,2-Hexanediol 1.00 moisturizer PEG-14M 1.20 solvent Polyquaternium-10 0.60 antistatic agent Ethylhexylglycerin 0.05 moisturizer Disodium EDIT 0.02 sequestering agent Cosmetic composition of Example 4 30.00 Hair loss prevention and hair growth promoter

Although the embodiments of the present invention have been described in detail as described above, the scope of the present invention is not limited thereto, and the scope of the present invention extends to those substantially equivalent to the embodiments of the present invention.

1: C57BL/6 mouse
2: Parietal part

Claims (5)

In the cosmetic composition for preventing hair loss and promoting hair growth,
The cosmetic composition comprises asiaticoside, bilberry extract, cheonsimryeon extract and soybean violet extract as active ingredients,
The asiaticoside comprises 0.01 to 5.0% by weight based on the total weight of the cosmetic composition,
The natural mixed composition consisting of the bilberry extract, cheonsimryeon extract and soybean violet extract contains 1.0 to 30% by weight based on the total weight of the cosmetic composition,
The natural mixture composition is a cosmetic composition for preventing hair loss and promoting hair growth, characterized in that the bilberry extract, cheonsimryeon extract and soybean violet extract are mixed in a weight ratio of 1: 1: 2.
The method of claim 1,
The cosmetic composition for preventing hair loss and promoting hair growth is an astringent lotion, softening lotion, lotion, cream, essence, pack, cleansing foam, cleansing cream, shampoo, conditioner, hair tonic, any one formulation selected from the group consisting of massage cream A cosmetic composition for preventing hair loss and promoting hair growth, characterized in that it has a.
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