KR20100011176A - Natural and oriental herbs complex with anti-hairloss and hair growth stimulating effects - Google Patents

Abstract

PURPOSE: A composition for preventing hair loss and promote hair growth using medicinal herb extract is provided to ensure low irritation to the skin and promote hair growth. CONSTITUTION: A cosmetic composition for preventing hair loss and promoting hair growth contains 10-25 weight% of Chrysanthemum indicum L., 10-25 weight% of Carthamus tinctorius, 10-25 weight% of Calendula arvensis, 10-25 weight% of Lonicera japonica Thunberg, 10-25 weight% of Inula Britannica var. japonica., and 0.1-5 weight% of badger oil as active ingredient. The medicinal herb extract is contained in 0.001-5 weight% of dry or 0.001-50.00% of liquid phase. The medicinal herb extract is obtained by adding 1 to 15 times volume of extract solvent to Chrysanthemum indicum L., Carthamus tinctorius, Calendula arvensis, Lonicera japonica Thunberg, Inula Britannica var. japonica., and badger oil, heating at 80-100°C for 8 to 48 hours, extracting at 4-10°C for 3 to 20 days. The extraction solvent is water, ethanol, ethylacetate, glycerin, ethyleneglycol, butyleneglycol, propyleneglycol, hydrous ethano, hydrous glycerin, hydrous ethyleneglycol, hydrous butyleneglycol, or hydrous propyleneglycol. The composition is used in the form of shampoo, rinse, hair gel, hair tonic, hair lotion, soap, or hair pack.

Description

Composition for preventing hair loss and promoting hair growth using herbal extracts {Natural and oriental herbs complex with anti-hairloss and hair growth stimulating effects}

The present invention relates to a composition for preventing hair loss and promoting hair growth using the herbal complex extract.

Hair is not directly related to life, but it provides sexual appeal and protects the scalp from sunlight.

While many hairy animals renew their appearance with regular molting, people can't see any obvious changes because they lose their hair little by little every day. This is because the animal's hair is all the same cycle, so if the hair falls out, the entire time is missing, but because the human hair cycles are all different, only some of the hair falls out every day. The hair grows and falls in a constant cycle and continues to produce different types of hair in the same hair follicle, not just like a fingernail or toenail, which continues to grow throughout life, but where it grows for a certain period of time and then exits and exits again. Started hair grows. This growth cycle is called the mother cycle, and it is produced and destroyed repeatedly with periodic changes of the anagen, the catagen, the telogen, and the degenerator.

Anagen is a period in which hair continues to grow, with active cell division in hair follicles, or hair follicles. Catagen is a time when hair follicle growth activity ceases and rapidly contracts, and the shape of the hair becomes similar to the club.

Telogen hair follicles cease to be active and hair loss begins in the exogene. At the base of resting hair, new hair grows, and the existing hair is pushed out by the hair. When the hair is pulled from the normal scalp, about 85 ~ 90% of the hair is growing, 10 ~ 15% is the resting period, and degenerative hair is hard to find because of the small number. There are about 140,000 blondes, about 100,000 brown hairs, about 90,000 red hairs, and less than 100 hairs per day are newly grown.

Hair loss refers to a condition in which hair falls out more than new hairs. Hair loss is classified into scars and non-scars.

Scar loss is caused by the destruction of hair follicles and fibrous tissue, resulting in permanent hair loss, which is caused by trauma such as burns and wounds, radiation dermatitis, infections, folliculitis, pore squamous lines, lupus erythematosus, scleroderma, and tumors.

On the other hand, non-cancerous alopecia is alopecia in which tissue is not fibrous and hair follicles are preserved as it is, such as alopecia areata, masculine alopecia, growing hair alopecia, and hair growth walls. However, non-escarious alopecia can also be converted to scarring if the disease continues, and if scarring alopecia is not treated medically.

The cause of hair loss varies from person to person, but may be due to degeneration of the scalp cells due to aging, lack of nutrients to the hair roots, lack of oxygen supply due to excessive scalp fat or scalp disease, including genetic and hormonal effects. Inflammation, pore closure, and stress.

Alopecia areata is a symptom of over 90% of alopecia areata, and one or two or three round or oval alopecias occur suddenly, not only on the scalp but also on the eyebrows, eyelashes, and beards. In some patients, alopecia areata progresses to frontal alopecia or general alopecia areata.

The most commonly used treatments are corticosteroids, which are classified in the form of injections (muscle or intravenous and intralesional injections), oral tablets, external ointments or fluids, and the treatment formulation varies depending on the symptoms of alopecia areata.

However, corticosteroids have side effects such as acne, stretch marks, and irregular menstruation.

Androgenetic alopecia is caused by male hormone alopecia, and the scalp of the patients with this symptom is greasy and greasy, which is due to the development of sebaceous glands due to hormonal effects, which secrete sebum a lot, resulting in seborrheic dermatitis. Further promote. The prognosis is determined by genetic factors and male hair loss tends to be more severe as hair loss begins at a younger age. Minoxidil and finasteride, recently approved by the US Food and Drug Administration (FDA), are currently used worldwide because they are known for their effectiveness and they do not completely cure baldness.

Testosterone (androgen) is synthesized and secreted in the adrenal cortex and gonads, the most potent and representative of which is testosterone.

Testosterone is converted to the active male hormone dihydrotestosterone by an enzyme called 5a-reductase. 5a-reductase is present in the hair roots, and dihydrotestosterone plays a role in shortening hair follicle growth and decreasing hair follicle size. In other words, the thick black hair is not enough to grow and become thinner and weaker, so that it falls out much faster than normal, and shortens the hair cycle, so the hair is initially weak and thin and gradually loses its hair and does not become new.

Thus, male alopecia is a genetic disease in which various genes are expressed by interacting with certain environmental factors. In addition to testosterone, growth factors such as cytokines, epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factor-α (TGF-α), and TGF-β also affect hair growth.

Inflammatory findings are observed in about 50% of hair loss patients, which stimulate cytokines and growth factors. EGF and TGF-α promote the formation of hair-like structures and TGF-β inhibits hair growth.

In addition to genetic and physiological factors of hormonal hair loss, the causes of hair damage can be divided into physical and chemical causes. Physical is mainly caused by technical stimulus (brushing or reversal), heat, etc., but it is mainly morphologically changed, but there are cases in which qualitative damage is caused by chemical change in hair components by wave or dye.

Therefore, the present inventors have been researched to solve the above problems, the anti-oxidant effect is good, and prescribed in the optimal content of herbal extracts effective for hair loss and hair growth, almost no cell stimulation for human-derived keratinocytes, strong The present invention has been completed by developing an anti-hair loss and hair growth promoting hair cosmetic composition effective for preventing hair loss and wool, and hair loss through antioxidant activity and bacteriostatic activity.

Accordingly, an object of the present invention is to provide a hair cosmetic composition for preventing hair loss and promoting hair growth, including a complex extract of gamguk, safflower, marigold, gold, silver, and badger oil as an active ingredient.

The present invention is characterized by a hair cosmetic composition for preventing hair loss and promoting hair growth comprising a complex extract of gamguk, safflower, marigold, gold silver, sunbok and badger oil as an active ingredient.

Referring to the present invention in more detail as follows.

The present invention has been found that the extract of the mixture of gamguk, safflower, marigold, marigold, bokhwa, and badger oil has an excellent effect on antioxidant, hair loss prevention, and hair growth. This is about possible cosmetics.

Literature pharmacological action of the six herbal components used in the herbal hydrothermal extract composition is as follows.

Guam is the flower of chrysanthemum. The gun is 0.3 ~ 3cm in diameter and the gun has 3 ~ 4 layers. The outer surface of the gun is greenish brown or brown. The quality is light and brittle. It has a distinctive aroma and tastes slightly sweet and bitter. Flavonoid-based acecetin, acecetin-7-O-β-D-galactopyranoside and sesquiterpene (Sesquiterpene family of arteglasin A, chrysetunone, tunefulin). Gamyeok has an antipyretic effect and enhances the resistance of capillaries. It also has antibacterial and antiviral action. In addition, acetylene-containing compounds and flavonoids inhibit NO production in LPS-induced macrophages.

Safflower is either the coronation of Safflower (Carthamus tinctorius L.) as it is or the removal of most of the yellow pigment. It is made of red corolla and yellow shipper and stamen. The length is around 10 mm. It has an unusual smell and a bit bitter taste. The pigments include carthamin, safflor yellow A, cartormin, tinctormine, and the like. Flavonoid-based carthamidin, isocarthamidin, neocartamine, neoocthamin, 6-hydroxykaempferol, 6-hydroxycamperol 3-glucoside (6-hydroxykaempferol 3-glucoside), 6-hydroxycamphorol 3,6-diglucoside (6-hydroxykaempferol 3,6-diglucoside), apigenin, quercetin, kaempferol, (-) -Driodictyl [(-)-driodictyl], contains sccutellarein. It also has N- (p-coumaroyl) serotonin [N- (p-coumaroyl) serotonin] and N-feruroylserotonin which are serotonin derivatives. Others include kinobeon A, alkanediol, and helianol. TancutominTinctomine has a Ca ²⁺ antagonistic effect that lowers blood pressure. In addition, it exhibits an dilating action by antagonizing the vasoconstriction of adrenaline or noradrenaline. Serotonin derivatives, N- (p-coumaroyl) serotonin and N-ferroylserotonin, have antioxidant activities. Triterpene alcohols, such as Helianol, inhibit inflammation.

Marigold is a representative species of annual plant of autumn planting blooming from spring to early summer. Native to southern Europe and the Mediterranean. Its height is 30 ~ 50 cm and yellow or orange double flowers similar to chrysanthemum bloom in April ~ June. Contains a lot of lutein (Lutein). Lutein belongs to xanthophylls in carotenoids and serves as a pigment for photosynthesis of plants. Lutein is also a powerful antioxidant. Has antibacterial and antimicrobial action, anti-inflammatory action, and has an excellent antioxidant effect.

Gold and silver coins are buds of honeysuckle. It is a small funnel-shaped bud, 15-35 mm long. Magnifying glass has light brown hairs, calyx is green with 5 ends, and the fragment is hairy and about 2 mm long. Flavonoids include luteolin, lonicerin, and ochnaflavone. Iridoid-based loganin, secologanin, and secologanin. Dimethylacetal, sweroside, secologanoside 7-methyl ester, loniceracetalide A and B, phenylalaninocecologa Phenylalaninosecologanin. Phenolic compounds include chlorogenic acid, isochlorogenic acid, and inositol. The extract was improved by administering chlorogeninic acid to experimental animals infected with influenza virus as well as bacteria such as Salmonella typhi, S. paratyphi and Escherichia coli. In addition, it exhibits anti-inflammatory action by inhibiting edema and the like of the foot and reducing the degradation of IκBα in the liver to inhibit the activity of NFκB. Oxhaflavones also inhibit the activity of phospholipase A2.

Lilac is a flower of Inula Japonica Thunb. The diameter is 10 to 15 mm and has a spherical shape. It is composed of several guns and arranged in a tile shape. It has an unusual smell, tastes sweet and bitter behind. Sesquiterpene lactone-based xanthanolide, inulicin, deacetylinulicine, bigelovin, ergolide, 2,3- 2,3-dihydroaromaticin and flavonoid-based patulintin 7-Ο- (6 ''-isobutyryl) glucoside [patuletin 7-Ο- (6 ''-isobutyryl) glucoside ], Isoraminetin 3-glucoside (isorhaminetin 3-glucoside), patulitrin (patulitrin) and the like. Pseudoguaianolides such as Bigelovin (0.46 μM), 2,3-dihydroaromaticin, ergolide (1.05, 0.69 μM) iNOS inhibitory activity. Ergolides inhibit the expression of iNOS and COX-2 by inhibiting NFκB.

Badger (badger, scientific name: Meles meles) is a growing animal in oriental medicine, folk remedies and food. Recently, the Korea Forestry Research Institute developed the technology for mass production of food badger (1997), breeding permits and livestock permits (Ministry of Agriculture, October 17, 2001).

Badger's subcutaneous fat, along with seals and mink's subcutaneous fat, is used for burn treatment of folk remedies. This means that badger oil has the effect of promoting the growth of newly formed skin. In addition, the content of palmitic acid (palmitic acid) is very high, such as 14.0 ~ 18.0% by weight to promote the regeneration of the hydro-lipoid membrane of the dermal tissue, inhibiting bacteria invasion, harmful oxygen and UV protection, moisture evaporation It is effective in inhibiting sedimentation, improving blood circulation, improving skin circulation, etc., because it contains a large amount of unsaturated fatty acid (70 to 80%) to suppress the deposition of pigments due to pollutants and cell regeneration. Efficacy is recognized.

Extraction solvents of 1 to 15 volumes are added to a mixture of Gwakku, safflower, marigold, gold and silver, and scallop oil, which are heated at 80 to 100 ℃ for 8 to 48 hours, or deposited at 4 to 10 ℃ for 3 to 20 days. Extraction is followed by concentration. At this time, more preferably 10 to 25% by weight, 10 to 25% by weight, marigold 10 to 25% by weight, gold and silver 10 to 25% by weight, 10 to 25% by weight and badger oil 0.1 to the total extract composition It is advisable to mix at -5% by weight. At this time, outside the above range is not preferable because there is a problem of preventing hair loss and promoting hair growth, increasing skin irritation and cytotoxicity. The extraction solvent is one or two selected from the group consisting of water, ethanol, ether acetate, glycerin, ethylene glycol, butylene glycol, propylene glycol, hydrous ethanol, hydrous glycerin, hydrous ethylene glycol, hydrous butylene glycol, hydrous propylene glycol The above mixed solvent is preferable.

The composite extract thus prepared showed 3 to 4 times better hair loss prevention and hair growth promoting effect than a single extract, and 2 to 3 times better hair loss prevention and hair growth promoting effect than 2 to 5 complex extracts. In addition, it has a safe effect on skin irritation, and when applied to hair cosmetics, it can heal or improve skin troubles such as dandruff and scalp itch, and it will be more useful for people with chemical-sensitive skin because it uses natural materials. It is expected.

On the other hand, it is preferable to apply the complex extract as a hair product cosmetic, specifically, shampoo, rinse, hair gel, hair tonic, hair lotion, soap or hair pack, etc. as a dry weight with respect to the total weight of the hair cosmetic composition 0.001 ~ 5 It is blended in an amount of 0.001 to 50.00% by weight or liquid weight.

The composition according to the present invention, gamguk, safflower, marigold, marigold, bokhwa, badger oil complex extract is a natural substance, there is no objection to consumers, hypoallergenic and excellent safety on the skin, as well as prevent hair loss and promote hair growth It was effective, and compared with the conventional hair cosmetics, hair loss prevention and hair growth can be promoted, and it was possible to mix | blend and use in various hair cosmetics.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited thereto.

Example 1: Preparation of Complex Extract

Herbal medicines used in the present invention are composed of six herbal ingredients with high antioxidant effect through the selection of medicines and previous tests through data research, and the herbal medicine name, scientific name and content are shown in Table 1 below.

Chinese medicine renaming Scientific name and use area Content (wt%)    Persimmon Chrysanthemi Flos 20    Safflower Carthami flos 20    marigold Calendula arvensis 20    Gold coins Lonicerae Flos 20    Conquest Inulae Flos 19    Osioil Meles meles One

In order to obtain a flower composition (Flowers complex) excellent in hair growth and hair loss prevention effect used in the present invention, the herbs of Table 1 were sufficiently washed in purified water, and then mixed in the content ratio shown above. In order to obtain 10 L of herbal hydrothermal extract composition in a specific manner, 20 g of sauerkraut, 20 g of safflower, 20 g of marigold, 20 g of gold and silver coin, 19 g of ginseng and 1 g of oriorioyl were used. The hot water extract was heated at 90 ° C. for about 30 hours in a boiling water distillation machine. A cold water cooling tube of 7 ° C. was used to obtain distilled components volatilized at the extraction temperature during the extraction process. Specifically, vapors volatilized during hot water extraction were condensed while passing through a cold water cooling tube and recovered in a hot water still. The obtained composite extract was centrifuged at 12,000 rpm for 4 minutes for 30 minutes to remove solids, and the supernatant was used in the following test example.

The composite extract used in the present invention, respectively, by obtaining the respective herbal medicines in the same manner as described above, respectively, and then mixed in the content ratios shown in Table 1 showed the same hair growth and hair loss prevention effect.

Test Example 1: Human keratinocyte cell culture and cytotoxicity test

HaCaT cell line (Cell Line Service, Germany), a human keratinocyte, was cultured in a 37 ° C., 5% CO ₂ incubator for this study. When the degree of cultivation of 85 to 90% of the culture vessel was shown, the cells were detached by trypsin treatment, counted, and subcultured at 5 × 10 ³ cells / cm ² . Cell cultures were Delbecco's Modified Eagle Medium (DMEM, GIBCO, Cat.No. 10% FBS (GIBCO, Cat. No., 26140-079, USA) plus 100 U / ml penicillin and 100 μg / ml streptomycin). 11995-065, USA). 75 T-flasks (NUNC, Cat. No. 156499, Danmark) were used for subculture and 24 well plates (NUNC, Cat. No., 142475, Danmark) were used for cytotoxicity testing.

The MTT assay is a method of measuring the cytotoxicity of a test substance by finally forming a pigment product called promazan by using the dehydrogenase action of mitochondria which is active only in living cells.

In order to confirm whether the complex extract used in the present invention can show hair protection and hair loss prevention effects without scalp irritation even during long-term use, it was tested for cytotoxicity against human keratinocytes. Human keratinocytes cultured in the manner described in the section “Human keratinocyte cell culture” were counted using a heamacytometer and then aliquoted equally at 5 × 10 ³ cells / well in 24 well plates. After 48 hours of incubation in DMEM containing 10% FBS and incubated by 25 to 30% of the surface of the culture vessel, the complex extract of the present invention was replaced with FBS-free DMEM containing 10% to 0.1% for 24 hours. After incubation, 2.5 μl / ml of MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide, Sigma M5655, USA) solution was added 50 μl and incubated further for 3 hours. Subsequently, all of the cell culture solution was discarded, 200 μl of DMSO (Sigma D2650, USA) was treated with 200 μl of each well, followed by stirring. Then, 100 μl was taken as 96 wells and the absorbance was measured at 570 nm by ELISA. The degree of cytotoxicity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

An MTT test using a human-derived keratinocyte cell line HaCaT was performed as an indirect assessment of scalp stimulation that could be prolonged in long-term use. Initial cultivation 5 × 10 ³ cells per well was added to the composite extract up to 10% at the time when 25 to 30% of the surface area of the culture vessel, the cytotoxicity compared to the control is shown in FIG. There was no statistical difference between untreated group and 1% treated group. This result implies that scalp irritation due to cytotoxicity is not induced even when the complex extract is used for a long time.

Test Example 2: Antioxidant Test Using DPPH

One of the main mechanisms for the protective effect of the complex extract used in the present invention was the degree of antioxidant activity.

Compound DPPH (1,1-diphenyl-2-picryl hydrazyl, Sigma D9132-1G, USA) generates free radicals in ethanol. It was confirmed how much the amount of free radicals produced by mixing with the composite extract of Example 1 in a predetermined ratio. Briefly, 0.4 ml of ethanol was added 0.5 ml of 0.1 mM DPPH solution prepared with ethanol and 0.1 ml of the composite extract to a final concentration of 10% to 0.1%. It was strongly vortexed for 10 seconds and stored for 30 minutes in a cool dark place. Absorbance was measured at 517 nm using ELISA. The degree of antioxidant activity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

Contaminants from environmental pollution and chemical factors in excessive hair products are the main culprit of excessive oxidative stress on the hair. Such excessive oxidative stress may convert the growing hair into a resting state, and variously worsen the hair condition and may cause hair loss. Oxidative stress is also known to be a cause of alopecia areata as a mechanism for activating type 2 beta-corticotin secretion hormone receptors around hair follicles. It was confirmed by the free radical scavenging ability of DPPH whether the complex extract used in the present invention can have a hair growth and hair loss prevention effect by showing an antioxidant capacity, the results are shown in FIG.

As shown in FIG. 2, the DPPH free radicals of the reaction system were 83.78%, 47.92% when treated with 5%, 11.92% when treated with 1%, 5.39% when treated with 0.5% compared to the control group using purified water. I could confirm it. These results show that when the complex extract is applied to hair products such as shampoos, even when used in a high content of 1 to 10%, it has a small amount of cytotoxicity and can have hair growth and hair loss prevention effects with significant antioxidant capacity.

In particular, the extract was prepared by the method of Example 1, but the results of confirming the DPPH antioxidant activity of the single extract extracted 100 g each of the six herbal medicines, it is shown in FIG. As a result, the composite extract of the present invention showed a three to four times better DPPH antioxidant effect than a single extract, which shows that the hair growth and hair loss prevention effect in the composite extract can have a superior effect than the single extract.

Test Example 3: The bacteriostatic ability through the remaining live bacteria test

One of the main causes of damaged hair is unclean scalp conditions such as oxidative stress caused by pollutants such as air pollution and dandruff caused by abnormal dandruff transition.

The complex extract of the present invention was confirmed whether the bacteriostatic ability for which microorganisms do not survive without preservative treatment is present against bacteria and fungi. In order to confirm the number of viable bacteria in the complex extract, the number of viable bacteria was tested immediately after the extract was prepared and left at room temperature for 30 days. 0.1 ml of a briefly prepared hot water extract solution was plated in a retinal agar medium (Difco, USA) and incubated at 30 ° C. for 48 hours to confirm the presence of bacteria, and 0.1 ml of sabrowood dextrose agar medium (Difco, USA). ) And incubated for 72 hours at 25 ℃ to confirm the presence of fungi.

Dandruff, which can cause hair loss and hair damage as an inhibitor of scalp cleanness, can be thought of as a replacement of normal scalp cells. However, as the transition of fungal dandruff-producing bacteria such as pytyrosporum increases due to various causes such as various oxidative stresses today, the occurrence of dandruff may be remarkably increased and hair damage may be caused. Through the spontaneous bacteriostatic activity of the complex extract used in the present invention, it was confirmed whether it is possible to inhibit the transition of microorganisms without the addition of preservatives, the results are shown in FIG. Immediately after the preparation of the composite extract, the presence of bacteria (A) and fungi (B) was confirmed by the method of culturing after plating on agar medium.

The bacteriostatic ability of inhibiting the transition of microorganisms without the addition of preservatives even in long-term storage at room temperature may allow the complex extract to prevent hair loss and promote hair growth by inhibiting abnormal transitions such as dandruff producing bacteria.

Test Example 4: Determination of the effect of promoting hair growth using a mouse

In order to determine the effect of the composite extract used in the present invention, the hair growth promoting effect of the composition was confirmed using C57BL / 6N mice. On 49-51 days of age when the C57BL / 6N mouse enters the second catagen, the hair of the back is completely removed using wax and the extract of Example 1 is applied 100 μl twice a day for 15 days. By measuring the length of the hair according to the degree of hair growth promotion was compared.

As a control, pure water having no hair growth promoting effect was used.

The results are shown in Table 2 below, and FIG. 5 is a morphological change photograph showing the hair growth process over time as a representative example.

Elapsed material 6 days 10 days 14 days Treatment group (composite extract) 0.70 ± 0.2 mm 1.25 ± 0.21 mm 2.99 ± 0.24 mm Control group (pure water) 0.63 ± 0.25 mm 1.08 ± 0.13 mm 2.01 ± 0.35 mm

Test Example 5: Skin irritation test

Most herbal extracts have less skin irritation than common chemicals, but they can cause skin problems or scalp itching if the harmful substances are not removed properly. Thus, in order to measure the degree of skin irritation of the complex extract used in the present invention, a patch test was conducted on 11 subjects. A patch containing 100 µl of the complex extract and the control was attached to a clean area inside the arm of the subject, washed 48 hours later with running water, and then the degree of stimulation was determined after 30 minutes. The negative control group was pure water, and the positive control group was 5% sodium laureth sulfate.

Evaluation of the skin response was determined by dividing the stimulus value into five stages, it was confirmed that the skin irritation of the complex extract of the present invention was 2.2 in the negative control group, 54.5 in the positive control group, 6.8 of the complex extract was almost no skin irritation .

division Stimulation level Skin irritation average degree (%) Irritancy judgment 4 3 2 One 0 Negative Control 0 0 0 One 10 2.2 One Positive control group 0 4 5 2 0 54.5 4 Complex extract 0 0 0 3 8 6.8 One

 * Irritation Determination: 1 (less than 10, minimum stimulation), 2 (10-20, mild stimulation) 3 (20-30, moderate stimulation), 4 (more than 30, severe stimulation)

Preparation of Cosmetics Using Complex Extracts of Gamguk, Safflower, Marigold, Gold and Silver, Bokhwa and Badger Oil

Formulation Example 1: Preparation of Shampoo

Prescription examples of shampoo in cosmetics containing gamguk, safflower, marigold, gold and silver, sunbok, badger oil complex extract is shown in Table 4.

number Raw material name Prescription Example 1 Comparative Example 1 One  Complex extract 20.00 - 2 Sodium Lauryl Sulfate Solution (25%) 25.00 25.00 3 Sodium lauryloxyethylene sulfate solution (25%) 25.00 25.00 4 Cocamidopropyl Betaine 10.00 10.00 5 Panthenol 0.10 0.10 6 Polyquaternium-10 0.50 0.50 8 Hydroxypropyltrimonium chloride 0.20 0.20 9 Cocamide Diei 3.00 3.00 10 Ethylene Glycol Distearate 2.00 2.00 11 Butylene Glycol 1.00 1.00 12 Citric acid 0.10 0.10 13 antiseptic Quantity Quantity 14 Spices Quantity Quantity 15 Purified water Remaining amount Remaining amount

Formulation Example 2: Preparation of Rinse

Prescription examples of the rinse in cosmetics containing a mixture of gamguk, safflower, marigold, gold silver, sunbok, badger oil is shown in Table 5.

number Raw material name Prescription Example 2 Comparative Example 2 One  Complex extract 20.00 - 2 Disodium ID 0.05 0.05 3 Panthenol 0.10 0.10 4 Polyquaternium-10 0.30 0.30 5 Glyceryl Stearate / Pig-100 Stearate 1.50 1.50 6 Polysorbate 60 0.50 0.50 7 Glyceryl Stearate 0.40 0.40 8 Stearyl alcohol 3.00 3.00 9 Dimethicone 2.00 2.00 10 Mineral oil 3.00 3.00 11 Cetyl alcohol 2.00 2.00 12 antiseptic Quantity Quantity 13 Spices Quantity Quantity 14 Purified water Remaining amount Remaining amount

Formulation Example 3: Preparation of Hair Gel

Prescription examples of hair gels in cosmetics containing gamguk, safflower, marigold, marigold, sunbok, and badger oil complex extracts are shown in Table 6 below.

number Raw material name Prescription Example 1 Control One  Complex extract 5.00 - 2 Disodium ID 0.05 0.05 3 Panthenol 0.10 0.10 4 glycerin 5.00 5.00 5 Carbomer 0.60 0.60 6 Hydroxyethyl cellulose 0.30 0.30 7 ethanol 7.00 7.00 8 Polyoxyethylene Cured Castor Oil 1.00 1.00 9 Triethanolamine 0.60 0.60 10 Polyvinylpyrrolidone 5.00 5.00 11 antiseptic Quantity Quantity 12 Spices Quantity Quantity 13 Purified water Remaining amount Remaining amount

Prescription 4: Preparation of hair tonic

Prescription examples of hair tonic in cosmetics containing gamguk, safflower, marigold, gold silver, sunbok, badger oil complex extract is shown in Table 7.

number Raw material name Prescription Example 1 Control One Complex extract 50.00 - 2 ethanol 35.00 40.00 3 Fiji-8 5.00 10.00 4 Methylgluses-20 3.00 5.00 5 Panthenol 0.50 0.50 6 Fiji-32 2.00 3.00 7 Sorbitol 1.00 2.00 8 Polyoxyethylene Cured Castor Oil 0.50 0.50 10 antiseptic Quantity Quantity 11 Spices Quantity Quantity 12 Purified water Remaining amount Remaining amount

Figure 1 shows the cytotoxicity results of the complex extract according to the present invention.

Figure 2 shows the free leg scavenging ability of DPPH for the complex extract according to the present invention.

Figure 3 compares the antioxidant capacity of DPPH for a complex extract and a single extract according to the present invention.

Figure 4 shows the bacteriostatic ability of the composite extract according to the present invention.

5 is an external morphological change picture showing the hair growth process over time.

Claims (6)

Hair cosmetics for preventing hair loss and promoting hair growth, characterized in that it comprises a complex extract of gamguk, safflower, marigold, gold and silver, sunbok and badger oil as an active ingredient. The method of claim 1, wherein the complex extract is 10 to 25% by weight, 10 to 25% by weight safflower, 10 to 25% by weight marigold, 10 to 25% by weight gold and silver, 10 to 25% by weight, badger oil 0.1 ~ A hair cosmetic composition, characterized in that 5% by weight of the mixture is extracted with an extraction solvent. The method of claim 1, wherein the complex extracts of the gamguk, safflower, marigold, marigold, bokhwa and badger oil are dried in an amount of 0.001 to 5% by weight or 0.001 to 50.00% as a liquid weight based on the total weight of the hair cosmetic composition. Hair cosmetic composition, characterized in that it is included. According to claim 1, wherein the complex extracts of the gamguk, safflower, marigold, gold and silver, bokhwa and badger oil is 1 to 15 vol. The hair cosmetic composition, characterized in that prepared by heating for 8 to 48 hours at 80 ~ 100 ℃, or by dipping for 3 to 20 days at 4 ~ 10 ℃, then concentrated. The method of claim 4, wherein the extraction solvent is composed of water, ethanol, ether acetate, glycerin, ethylene glycol, butylene glycol, propylene glycol, hydrous ethanol, hydrous glycerin, hydrous ethylene glycol, hydrous butylene glycol, hydrous propylene glycol Hair cosmetic composition, characterized in that one or more mixed solvents selected from the group. The hair cosmetic composition according to any one of claims 1 to 5, having a formulation of a shampoo, rinse, hair gel, hair tonic, hair lotion, soap or hair pack.

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