KR101332215B1 - Cosmetic composition containing fermentated complex of extracts with cryptomeria japonica and nelumbo nucifera for acne improvement and enhancing immune system - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a complex fermented extract which treats acne and enhances skin immunity with superior safety, and more specifically, to a cosmetic composition containing a complex fermented extract containing Cryptomeria japonica and lotus leaves as an active ingredient for treating acne and enhancing skin immunity.

Description

Cosmetic composition for acne improvement and immune enhancement containing cedar and lotus leaf fermentation extracts as an active ingredient {Cosmetic composition containing fermentated complex of extracts with Cryptomeria Japonica and Nelumbo Nucifera for acne improvement and enhancing immune system}

The present invention relates to a cosmetic composition containing a complex fermented extract that is excellent in safety and effective in improving acne and enhancing skin immunity. More specifically, the present invention relates to a cosmetic composition having an improved effect on skin acne and skin immunity, including a complex fermentation extract containing cedar and lotus leaf among natural products as an active ingredient.

Acne is a skin disease in which excessive sebum caused by the active activity of sebaceous glands is accumulated in the pores and does not escape smoothly, but is combined with bacteria in the pores and progresses to inflammation. 70 to 80% of patients are 11 to 25 years old. Focused on the age group, mild acne can be said to be one of the skin characteristics of the growing season rather than skin disease. The causes of acne can be largely explained according to the degree of development of acne symptoms such as imbalance of hormone secretion, increase of sebum secretion, narrowing of hair follicle hyperkeratosis, hyperplasia of acne bacteria, inflammatory reaction, etc. Different mechanisms may also work in combination.

Looking at the main causes of occurrence,

First is the change in hormones. Testosterone, a type of male hormone (androgen), stimulates sebaceous glands to promote sebum secretion, increasing sebum volume, and at the same time thickening the keratin of the pores wall to prevent sebum from being discharged smoothly, causing acne. This male hormone is secreted during puberty, so acne will occur a lot. This male hormone is not only released to men, but also to females secrete an adequate amount of male hormones at a certain rate. When the balance of these hormones is disturbed, it causes acne.

Second is the increase in sebum secretion. When the sebaceous glands are stimulated by androgen hormones, they produce more sebum. The oil-based sebum accumulates in the hair follicles and then rises up the skin surface along the hair wall to be discharged out of the skin, where the sebum is mixed with the normal skin bacteria bacteria or dead skin cells that have fallen out of the skin cells that make up the inside of the hair follicles. do. When excessive sebum is produced, it can't get out of the skin and part of it accumulates in the hair follicles, leading to acne lesions.

Third, changes in hair follicles. As the production of androgen hormones increases and the sebaceous glands become larger, changes in the skin that make up the inner wall of the hair follicles occur. Normally, the dead cells in the hair follicles come out of the skin one by one, but in puberty acne patients, these cells drop out so rapidly that they do not come out all together and are entangled with sebum to block the hair follicles.

Fourth, the growth of acne bacteria. Hair follicles blocked by sebum and dead skin cells create a good environment for bacteria to grow. Characteristically, in acne, a bacterium called propionibacterium acne is the main cause of acne when it grows on sebum. In normal people without acne, the bacteria are normally present on the skin and do not cause any problems. Clogged sebum accumulates in the hair follicles eat sebum quickly grow up. Various chemicals produced by these bacteria can cause inflammation of hair follicles and surrounding skin.

In addition, indirect causes of stress, lack of sleep, drinking, food, cosmetics, physiology, drug side effects are related to acne. These causes the skin to become less resistant and cause sensitive or purulent acne.

To treat such acne, there is a method of using medicines in the form of oral or topical agents. Specifically, the method of inhibiting sebum production using anti-androgenic agents, treatment with anti-inflammatory action of steroid hormones and nonsteroidal anti-inflammatory drugs, including lesochinol, sulfur, salicylic acid, benzoyl peroxide, retinoic acid or isotretinoin There is a method for inhibiting the activity of propionibacterium acne bacteria by antibiotics such as antimicrobial agents and exfoliants, tetracycline, erythromycin, and macrocycline. Recently, treatment with azelic acid has also been widely used.

However, the above-described methods have some results in acne treatment, but have some problems in terms of effectiveness, side effects, and usability. The use of hormones has been reported to have side effects such as inhibition of the growth of the epidermis, and the exfoliating agents such as retinoic acid and benzoyl peroxide have been reported to cause skin irritation and contact dermatitis, The substance is known to be resistant to bleeding and photosensitization of resistant bacteria. In particular, isotretinoin is known to have an ultrasound effect on the fetus.

Therefore, in acne skin, the need for the development of a product that is safe for the human body and has an improvement effect and can enhance the skin's immunity is steadily emerging.

Republic of Korea Patent Publication No. 2006-0111026 "Cosmetic composition for improving oily skin containing herbal extracts and a method for producing the same" contains the extract of rhubarb, red ginseng, rye lotus and Changsha of the skin pore contraction effect, anti-inflammatory effect and anti-acne A cosmetic composition for improving oily skin having an effect is disclosed, and Korean Patent Registration No. 0829729, "External skin composition containing Byeongsocho extract as a main active ingredient," has a `` outpost extract of Bisosocho with fern cedar ferns. External skin composition having excellent hair growth, acne prevention effect, anti-inflammatory effect, stimulating effect, characterized in that it contains as an active ingredient 'is disclosed.

The present inventors completed the present invention by confirming that the cedar and lotus leaf complex extracts have an excellent acne improvement effect while researching natural products having acne improving activity.

Accordingly, an object of the present invention is to provide a cosmetic composition having a cedar and lotus leaf-containing complex fermentation extract as an active ingredient having an improvement on skin acne and skin immune enhancement effect. In particular, it has antimicrobial activity that inhibits growth against P. acnes, inhibits Lipogenesis and promotes immune enhancement activation by increasing production of nitric oxide and TNF-α secreted from macrophage An object of the present invention is to provide a cosmetic composition having an acne improvement and skin immune enhancing effect.

According to the present invention to achieve the above object, there is provided a cosmetic composition for improving acne and skin immunity containing the fermented extract of cedar and lotus leaf. The complex fermentation extract is obtained by fermenting cedar and lotus leaf extract with yeast or lactic acid bacteria, respectively or a mixture thereof. Preferably, the yeast is Saccharomyces cerevisiae. At this time, the cedar and lotus leaf complex fermentation extract as an active ingredient is contained 0.1 to 10% by weight based on the total weight of the cosmetic composition. In addition, the cedar and lotus leaf complex fermentation extract is fermented and extracted by mixing the cedar and lotus leaf in the weight ratio of 1 to 2: 1 to 2, respectively, more preferably to be fermented by mixing in a ratio of 2: 1 . The cedar and lotus leaf extract is a hot water extract, or a solvent extract by at least one solvent selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol. More preferably an extract with 70% ethanol solvent.

In order to achieve the above another object, according to the present invention, (A) cedar and lotus leaf are mixed in a weight ratio of 1 to 2: 1 to 2 and water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene Preparing a mixed extract by extracting with at least one solvent selected from the group consisting of glycol and butylene glycol; (B) inoculating fermented yeast, lactic acid bacteria or mixtures thereof to the prepared mixture extract; And (C) after the sterilization is provided a method for producing a composite fermentation extract comprising the step of filtration and concentration.

Preferably, the step (B) consists of fermenting Saccharomyces cerevisiae (Saccharomyces cerevisiae) in the mixed extract for 4-6 days at 22-28 ° C.

The combined fermentation extract has antimicrobial activity that inhibits growth against acne bacteria (P. acnes), inhibits lipid synthesis and activates immune enhancement by increasing production of nitric oxide and TNF-α secreted from macrophage (Macrophage) It has the characteristics of acne improvement and skin immune enhancing effect by promoting.

The complex fermentation extract may be applied as a raw material in the form of a solution or powder by submicron encapsulation, microencapsulation or liposome.

In addition, the cosmetic composition containing the cedar and lotus leaf complex extract extract skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence , Pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow can be applied in any one formulation selected from the group consisting of.

Cosmetic composition comprising a composite fermented extract of cedar and lotus leaf is effective in improving acne and immune enhancing activity, in particular, the effect of inhibiting the antibacterial activity and lipid synthesis (Lipogenesis) to inhibit the growth of acne bacteria (P. acnes) It is excellent in promoting nitric oxide and TNF-α secretion effect of macrophages. The cosmetic composition containing the cedar and lotus leaf fermented extract is useful for acne improvement and cosmetic composition for skin immunity enhancement.

The present invention relates to a cosmetic composition for acne improvement and skin immunity enhancement containing a complex fermentation extract of cedar and lotus leaf as an active ingredient.

The cedar tree (Cryptomeria japonica) used in the present invention is an evergreen tree with larch, which is cold-resistant and grows only in the southern region, and its origin is Japan, but it is planted a lot in windproof forests in Jeju, etc. in Korea, and grows up to 70m in height and 4m in diameter. The leaves are needle-shaped, 0.5 ~ 1cm. Cedar is rich in phytoncide, which is widely known for its antimicrobial effects. It is a substance released by plants to resist pests, germs and bacteria, and has been widely used to cure harmful bacteria from ancient times. It has been widely used for allergic skin diseases such as swelling and atopic dermatitis.

The lotus leaf used in the present invention is also known as the perennial plant of the genus Amaniiaceae, also known as lotus (Nelumbo nucifera), and contains amino acids such as vitamin C and vitamin B12 and asparagine, arginine, tyrosine, etc., to improve fatigue and skin beauty. It is effective, as well as analgesic sedation of roemerine and nuciferine, which is effective for nervousness and stress, and in the folk, as well as pneumonia, bronchial asthma, gonorrhea, tonic, dyspepsia, It is known to use when bitten by poisonous insects. Lotus leaves are harvested from early June to mid-October. Normally, lotus leaves start in early June and fall in mid-October. Lotus leaf used in the present invention, also known as the lower lobe (荷葉) and as a pharmacological action is used for diarrhea, headache and dizziness, hemostasis, nosebleeds such as bleeding, postpartum blood treatment, enuresis, and neutralizes various toxic substances . In particular, smokers have a detoxifying action to remove nicotine. In addition, lotus leaf generally clears blood with detoxification effect, hemostasis, antibacterial action and blood pressure lowering effect. Especially, in the synonymous bogam, lotus leaf is called especially hobby and this part is pregnant Reminds a woman that it has the effect of relaxing the womb and eliminating bad blood. Teas using lotus leaf include Chinese whale Gambibi (減肥 減) (containing lotus leaf, yulmu, mountain lion, dermis, etc.), which is known to be effective for diet and skin beauty.

The cedar and lotus leaf fermentation extracts of the present invention have antimicrobial activity that inhibits growth against P. acnes, inhibit Lipogenesis and produce nitric oxide and TNF-α secreted from macrophage By increasing the activation of immune boosting has an effect of improving acne and skin immunity.

According to a specific example of the present invention, the cedar and lotus leaf mixed extracts are prepared by extracting with water, one or more solvents selected from anhydrous or hydrous alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol. . Alternatively, according to another specific example of the invention, the cedar and lotus leaf mixed extract may be cut into cedar and lotus leaf, and then water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonite It is extracted with one or more solvents selected from the group consisting of reel, petroleum ether and diethyl ether.

The cedar and lotus leaf mixture extract thus prepared is fermented with yeast, lactic acid bacteria or mixtures thereof. Preferably, the yeast is Saccharomyces cerevisiae. Skin absorption is promoted by the fermentation of the microorganism, and the effect of improving acne and skin immune enhancing activity is further increased.

In the cosmetic composition of the present invention, the cedar and lotus leaf fermented extract may be added to the cosmetic composition in an amount of 0.001 to 10.0% by weight based on the total weight of the cosmetic composition, preferably in an amount of 0.001 to 5.0% by weight Can be added to. The cedar and lotus leaves are each fermented and extracted by mixing in a weight ratio of 1 to 2: 1 to 2, more preferably, when the fermented extract is mixed in a ratio of 2: 1 to the growth of acne bacteria (P. acnes) It was the most effective in inhibiting antimicrobial activity and lipid synthesis (Lipogenesis) and skin immune enhancing activity.

The cedar and lotus leaf fermentation extract may be applied to the raw material in the form of a solution or powder by submicron encapsulation, microencapsulation or liposomes. In addition, the cosmetic composition containing the cedar and lotus leaf complex extract extract skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence , Pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow can be applied in any one formulation selected from the group consisting of.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Example 1 Cedar and Lotus Leaf Mixed Extract Preparation

Each cedar leaf and lotus leaf were collected, dried, and then washed with 50 g of dried cedar leaf and 50 g of lotus leaf (dry weight ratio = 1: 1), and 1 kg of 70% hydrous ethanol was added. Boil for hours and extract. Subsequently, the filtrate was filtered twice with Edbentech 5 filter paper and GFC 47 mm filter paper. After concentrating at 50 ° C. using a vacuum concentrator, the mixture was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry extract of 7.5 g in dry weight.

Examples 2 to 3: Cedar and lotus leaf mixed extract preparation

To prepare a mixed extract by extracting and filtering in the same manner as in Example 1 by varying the mixed weight ratio of cedar leaves and lotus leaf as shown in Table 1 below.

Example 4: Cedar and Lotus Leaf Fermented Extract Preparation

3kg of 70% hydrous ethanol was added to a sample of 50g of dried cedar leaves and 50g of lotus leaves (dry weight ratio = 1: 1), and dried at 100 ° C in an extractor equipped with a cooling condenser. Boil for hours and extract. After inoculation of Saccharomyces cerevisiae (Saccharomyces cerevisiae) to the extract was fermented at 25 ℃ for 5 days, sterilized for 2 hours at 95 ℃ and then filtered with 300 mesh filter paper, the precipitate was left at room temperature for one day to filter the sediment of Edbentech 5 Was filtered twice with Whatman GFC 47 mm filter paper. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a combined fermentation extract having a dry weight of 6.8 g.

Examples 5-6 6: Cedar and Lotus Leaf Fermented Extract Preparation

As shown in Table 1, the mixed weight ratio of cedar leaf and lotus leaf was changed to extract, ferment and filter in the same manner as in Example 4 to prepare a composite fermentation extract.

(Weight ratio) Mixed extract Complex fermentation extract Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Cedar One One 2 One One 2 A lotus leaf One 2 One One 2 One

Comparative Example 1: Preparation of Cedar Extract

After grinding the dried cedar leaves in the shade, 100g was put in 1kg of 70% hydrous ethanol and extracted by boiling at 100 ℃ for 8 hours in an extractor with a cooling condenser. Subsequently, the filtrate was filtered twice with Edbentech 5 filter paper and GFC 47 mm filter paper. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a cedar extract having a dry weight of 6.2 g.

Comparative Example 2: Preparation of Lotus Leaf Extract

After grinding the dried lotus leaf in the shade, 100g was put in 1kg of 70% hydrous ethanol and extracted by boiling at 100 ℃ for 8 hours in an extractor with a cooling condenser. Subsequently, the filtrate was filtered twice with Edbentech 5 filter paper and GFC 47 mm filter paper. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry leaf extract of 7.8 g in weight.

The cedar extract and lotus leaf extract prepared in Comparative Examples 1 and 2, the cedar and lotus leaf mixed extract prepared in Examples 1 to 3 and the cedar and lotus leaf composite fermented extract prepared in Examples 4 to 6 in dimethyl sulfoxide Dilution solution prepared by dilution was used for the experiment.

Test Example  1: acne bacteria (P. acne Antimicrobial activity test

The antibacterial effect of acne bacteria was compared with each extract made from cedar leaf and lotus leaf and triclosan as a positive control group as a chemical synthetic antimicrobial agent. Acne bacteria Propionibacterium acnes ATCC 6919 was used as test bacteria. The antimicrobial activity against P. acnes was determined by liquid medium dilution method. After diluting each sample at 100 ppm intervals from 0 to 1000 ppm in 2 ml of liquid BHI medium, 20 μl of 10 ⁶ CFU / ml of the inoculum was inoculated and incubated for 48 hours. The minimum concentration at which growth was inhibited based on turbidity was defined as the minimum growth inhibition concentration (MIC). The results obtained are shown in Table 2 below.

Minimum growth inhibitory concentration (MIC) sample Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 P. acne 1200 1700 1000 1200 1000 600 700 400

As shown in Table 2, it can be seen that each extract has an antimicrobial activity against acne bacteria, and among them, the antimicrobial activity of Example 6 (combined fermented extract prepared with a ratio of cedar leaf and lotus leaf 2: 1) is It was found to be particularly excellent.

Test Example 2 Lipogenesis Synthesis Inhibition Test

The sebaceous glands are many hamster biopsy (biopsy) the ear tissue in order to assess the degree of lipid synthesis inhibition on sebaceous gland by the method for evaluating the consumption of the carbon element (uptake) Lipid synthesis inhibitors to quantify the extent to the extent necessary for lipid synthesis is C ¹⁴ Incubated for 6 hours in a labeled medium and the degree of inhibition was assessed by comparative quantitation of the control and the sample group using a radiometer. As a sample group, 0.01% of the sample solution was added to the medium using hamster right ear tissue, and 0.01% of physiological saline was added to the medium using the hamster left ear tissue as a control group. The results are shown in Table 3. Inhibition rate in Table 3 was calculated by the following formula, the value is the average value performed for five hamsters.

Inhibition rate (%) = sample group / control group × 100

Lipogenesis inhibition assay sample Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Inhibition rate (%) 24 18 43 38 42 66 64 72

As shown in Table 3, it can be seen that each extract has an inhibitory effect on lipid synthesis, and among them, the lipid synthesis inhibition of Example 6 (combined fermented extract prepared with a ratio of cedar leaf and lotus leaf 2: 1). It was found that the effect was particularly excellent. Therefore, lipid synthesis is inhibited, and sebum is reduced, so that occurrence of acne can be suppressed.

Test Example 3: Nitric oxide measurement test

Macrophage (Raw 264.7 mouse cells) was dispensed into 96-well microplates at 4 × 10 ⁵ cells per well and treated with each extract at a concentration of 1000 ㎍ / mL, followed by an upper layer after 24 hours at 37 ° C and 5% CO _2. The solution was taken and measured for absorbance at 550 nm using Griess reagent to determine the concentration of nitric oxide. LPS was treated with 1 μg / ml as a control. The results obtained are shown in Table 4 below.

NO assay sample Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 NO generation
% Increase 14 8 22 17 23 46 44 58

As shown in Table 4, it can be seen that each extract has an effect of inducing NO production, and among them, the NO of Example 6 (combined fermented extract prepared using 2: 1 ratio of cedar leaf and lotus leaf) Induction of production of macrophages increased the activity of the immune system was found to be particularly good.

Test Example 4 TNF-α Measurement Test

Macrophages (Raw 264.7 mouse cells) were dispensed in 96-well microplates at 4 × 10 ⁵ cells per well and each extract was treated at 1000 ° C./ml at 37 ° C. and 5% CO ₂ for 24 hours. LPS was treated with 1 μg / ml as a control. The supernatant was taken and the amount of TNF-α was measured by sandwich ELISA. The results obtained are shown in Table 5 below.

TNF-α assay sample Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 TNF-α
Expression rate (%) 24 18 33 28 32 56 54 68

As shown in Table 5, it can be seen that each extract has an effect of inducing TNF-α, and among them, Example 6 (combined fermented extract prepared with a ratio of cedar leaf and lotus leaf 2: 1) is Induction of TNF-α production was shown to increase the activity of macrophages, the immune boosting effect was particularly excellent.

Important immune cells involved in the natural immune response are macrophages. Activated macrophages secrete inflammatory mediators and cytokines. We confirmed that the cedar and lotus leaf fermentation extracts were involved in innate immune responses through the activation of macrophages by confirming the increased production of NO and TNF-α, which are important mediators released from macrophages.

Example 7: Emulsion Base Preparation

Emulsion base was prepared according to the composition of Table 6 for the clinical test on the acne improvement effect and skin safety of the cosmetic containing the cedar and lotus leaf composite fermentation extract of the present invention.

Comparative Examples 3, 4 and 5: emulsion base preparation

To prepare a emulsion base containing only cedar and lotus leaf extract or no extract of the present invention as shown in the composition of Table 6 as a comparative example.

ingredient weight% Example 7 Comparative Example 3 Comparative Example 4 Comparative Example 5 glycerin 3.00 3.00 3.00 3.00 Disodium iodide 0.02 0.02 0.02 0.02 Polyglyceryl-3-methylglucoside distearate 1.50 1.50 1.50 1.50 Cetearyl alcohol 0.50 0.50 0.50 0.50 Caprylic / capric triglyceride 7.00 7.00 7.00 7.00 Polyacrylamide & C13-14 Isoparaffin
& Laures-7 0.60 0.60 0.60 0.60 Cedar and Lotus Leaf Fermented Extracts 1.00 - - - Cedar Extract - 1.00 - - Lotus Leaf Extract - - 1.00 - Incense, preservative a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance system 100 100 100 100

Test Example 5: Measurement of Improvement on Acne

Twenty women aged 19 to 26 years were allowed to use the emulsion bases of Examples 7, and Comparative Examples 3 to 5 for the same use as the daily lotion for 4 weeks. Was determined according to the following evaluation criteria. The test results are shown in Table 7.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: Some improvement.

±: No improvement but no deterioration.

-: No improvement, but worse.

sample 1 week 2 weeks 3 weeks 4 weeks Example 7 + ++ ++ +++ Comparative Example 3 ± + + + Comparative Example 4 ± ± + + Comparative Example 5 ± ± ± ±

As can be seen from Table 7, the emulsion base containing the cedar and lotus leaf fermentation extract was found to have a very good improvement on acne.

Test Example 6: Skin Safety Test

In order to confirm the skin safety of the cosmetic composition containing the cedar and lotus leaf fermentation extract of the present invention has not shown a hypersensitivity reaction to the skin irritation in the past, currently 20 women in their 20s to 30s without skin disease or skin allergy symptoms Human skin patch test was performed. The test site was first wiped with 70% ethanol and then dried. The materials of Example 7 and Comparative Examples 3 to 5 were added dropwise into the Fin chamber (Finn chamber, 100x 10, EPITEST, Finland) by 15 µg, and then sealed in the forearm inner part of the test subject. The patch was patched for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. The skin response was determined according to the regulations of the Internatiolnal Contact Dermaitis Research Group (ICDRG), and the average response rate was calculated according to the following formula, and the results are shown in Table 8.

Average skin reactivity = (score × number of responses × 100 × 1/2) × [3 (maximum score) × total number of subjects (n)]

* Criteria, preferences and scores of skin reactions

No response:-(0)

Faint or light erythema: ± (0.5)

Boundary but weak erythema, edema and papules: + (1)

Pronounced erythema, papules and hydrophobic: ++ (2)

Severe erythema and alveolar, skin formation: +++ (3)

Test substance After 1 hour 24 hours later Response (%)
(n = 20) ± + ++ ± + ++ Example 7 - - - - - - 0.00 Comparative Example 3 - - - - - - 0.00 Comparative Example 4 - - - - - - 0.00 Comparative Example 5 - - - - - - 0.00

As can be seen from Table 8, skin stimulation did not appear in the cosmetics containing the cedar and lotus leaf fermentation extracts were excellent skin safety.

Claims (7)

Cosmetic composition for acne improvement and skin immunity enhancement comprising the cedar and lotus leaf fermentation extract as an active ingredient. The cosmetic composition for acne improvement and skin immunity enhancement according to claim 1, wherein the complex fermentation extract is obtained by fermenting cedar and lotus leaf extracts with yeast, lactic acid bacteria or a mixture thereof. The cosmetic composition for acne improvement and skin immunity enhancement according to claim 1, wherein the cedar and lotus leaf fermentation extracts are fermented and extracted by mixing cedar and lotus leaf in a weight ratio of 1 to 2: 1 to 2, respectively. According to claim 1, wherein the composition is acne bacterium Propionibacterium acnes (Propionibacterium acnes) characterized in that the cosmetic composition for acne improvement and skin immunity enhancement. According to claim 1, wherein the composition is a cosmetic composition for improving acne and skin immunity, characterized in that to inhibit lipogenesis (lipogenesis). According to claim 1, wherein the composition is a cosmetic composition for improving acne and skin immunity, characterized in that the production of NO and TNF-α which is a medium secreted from macrophages. (A) at least one selected from the group consisting of cedar and lotus leaf in a weight ratio of 1 to 2: 1 to 2 and water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol and butylene glycol Preparing a mixed extract by extracting with a solvent;
(B) inoculating fermented yeast, lactic acid bacteria or mixtures thereof to the prepared mixture extract; And
(C) method of producing cedar and lotus leaf fermented extract comprising the step of filtration and concentration after sterilization.