CN108354884B - Facial mask capable of removing acne and preparation method thereof - Google Patents
Facial mask capable of removing acne and preparation method thereof Download PDFInfo
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- CN108354884B CN108354884B CN201810368537.1A CN201810368537A CN108354884B CN 108354884 B CN108354884 B CN 108354884B CN 201810368537 A CN201810368537 A CN 201810368537A CN 108354884 B CN108354884 B CN 108354884B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention belongs to the technical field of facial mask preparation, and particularly relates to a facial mask capable of removing whelks and a preparation method thereof, wherein the preparation method of the facial mask takes evening primrose, radix sophorae flavescentis and rice as raw materials, the evening primrose is prepared into an evening primrose fermentation extract by utilizing lactic acid bacteria, the radix sophorae flavescentis extract is prepared by utilizing the lactic acid bacteria and a high-pressure treatment mode of 100-200 MPa, the rice flour is mixed with filter residues after the radix sophorae flavescentis treatment and organic acid, and the rice extract is prepared by utilizing acid protease for enzymolysis after soaking; finally, mixing 30-50 parts of the evening primrose fermentation extract, 10-30 parts of the sophora flavescens extract and 500-1000 parts of the rice extract, and then carrying out high-pressure and cold storage treatment; the finally prepared mask has good oxidation resistance for inhibiting bacteria, removing grease and removing free radicals, can remove whelk, has obvious effect, and has no uncomfortable side effects such as irritation or red, swollen and swollen face and the like.
Description
Technical Field
The invention belongs to the technical field of facial mask preparation, and particularly relates to a facial mask capable of removing whelks and a preparation method thereof.
Background
Acne, also called facial acne, is a common skin disease, frequently occurring in teenagers. The main reasons for whelk include internal and external factors, wherein the internal factor is mainly an endocrine factor, which means that after whelk enters, the hormone in the body is increased to stimulate the secretion of the pilosebaceous gland, so as to block the pilosebaceous gland duct. External causes include improper diet, irregular life, improper cosmetics or improper facial cleaning, and aggravation of damp heat in the body and generation of acne easily caused by pungent taste, sweetness, greasiness, high fat and high calorie consumption, cold drink and the like; irregular life such as frequent staying up night easily causes in vivo metabolism disorder, causes skin water diversion and endocrine dyscrasia, causes dark complexion and acne; improper use of cosmetics or improper cleansing of the face may cause clogging of pores or bacterial infection, thereby inducing whelk. Acne is often a combination of the above factors.
Once whelk happens, the face beauty is affected, and the patient is subjected to greater psychological stress. According to the different degrees of whelk, facial cleansing treatment, hormone treatment, traditional Chinese medicine treatment and the like are generally available. The facial cleaning treatment is suitable for the condition of less acne, and the hormone treatment and the traditional Chinese medicine treatment aim at the condition of more acne. The facial cleaning treatment mainly uses sulfur soap for cleaning, and is used for cleaning facial grease and inhibiting bacteria. However, the sulfur soap has certain irritation side effects, so that the risk of sensitive and delicate skin injury is high. The hormone treatment medicine has large side effect and is easy to cause body fat, etc. The traditional Chinese medicine needs to be decocted, so that the preparation of a patient is troublesome, the quality guarantee period is short, and the taste is bitter.
In order to solve the problems, acne-removing and skin-care masks or face creams appear for years, and the skin care products have the effects of skin care and acne removal and are convenient to use. For example, chinese patent CN102366374B discloses an anti-acne natural extract composition and anti-acne cream prepared from the same, which comprises scutellaria baicalensis extract, honeysuckle extract, sophora flavescens extract, dandelion extract, glycerin, salicylic acid and the like, and has good oil-controlling anti-acne effects. But the raw materials of the acne-removing cream are various and too complex.
In conclusion, the prior art has the problem that an acne-removing skin care product with small side effect, convenient use and few raw material types is lacked for treating and preventing the condition of few acnes.
Disclosure of Invention
The invention aims to provide a facial mask capable of removing whelks and a preparation method thereof, and the facial mask capable of removing whelks has small side effect, is convenient to use and has few raw material varieties.
The invention provides a facial mask capable of removing whelks, which comprises the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Adding lactobacillus liquid into fresh whole plant of the Chinese sage herb, fermenting for 24-36 h at 30 +/-1 ℃, then adding 2g/L citric acid solution, sterilizing, filtering, and collecting filtrate, namely the Chinese sage herb fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus bacterial liquid and the citric acid solution is 10-30: 1: 50-100;
taking fresh radix sophorae flavescentis, cutting into slices, placing the slices in lactobacillus liquid, soaking for 10-12 h at 4 ℃, then treating for 2-3 min under the condition of 100-200 MPa, filtering, respectively collecting filter residues and filtrate, and sterilizing the collected filtrate to obtain a radix sophorae flavescentis extract;
s2, Rice extract
Grinding rice into powder, sieving to obtain rice powder, adding an organic acid solution and the filter residue obtained in S1 into the rice powder, uniformly mixing, standing for 1.5-2 h, adding acid protease, performing water bath enzymolysis at 60 ℃ for 30-50 min, and performing water bath enzyme deactivation at 95 ℃ to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 50-100: 100-150: 1;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 30-50 parts of a Chinese sage herb fermented extract, 10-30 parts of a sophora flavescens extract and 500-1000 parts of a rice extract;
mixing the weighed evening primrose fermentation extract, the sophora flavescens extract and the rice extract, concentrating the obtained mixture to be pasty, treating for 1-3 min under 100-200 MPa, and refrigerating for 4-6 h at 4 ℃ to obtain the mask capable of removing the acne.
Preferably, in the preparation method of the facial mask for removing whelk, in S1, the Lactobacillus is Lactobacillus plantarum ATCC 14917, and the viable count in the Lactobacillus bacterial liquid is not less than 106One per ml.
Preferably, the preparation method of the facial mask capable of removing whelks comprises the following steps: activating Lactobacillus plantarum ATCC 14917 seed, inoculating into culture medium, culturing at 30 + -1 deg.C until the viable count in the bacterial liquid is not less than 106Per ml; the culture medium is prepared according to the following method: 50ml of cow milk, 20g of cane sugar, 5g of evening primrose, 5g of fresh radix sophorae flavescentis slices and distilled water are added to a constant volume of 1L, and a culture medium is sterilized for later use.
Preferably, in the preparation method of the facial mask capable of removing whelks, the culture medium is sterilized at 121 ℃ for 15min when the lactobacillus bacterial liquid is prepared.
Preferably, in the method for preparing the facial mask capable of removing acne, in S2, the organic acid solution is 0.01mol/L acetic acid solution, 2g/L malic acid solution or a mixture of the two according to a volume ratio of 1: 1.
Preferably, in the method for preparing the facial mask capable of removing acne, in S1, the sterilization mode is boiling sterilization for 15 min.
Preferably, in the preparation method of the facial mask for removing acne, in S3, the specific operation mode of concentrating to paste is as follows: the mixture obtained in S3 is continuously stirred and heated to evaporate water until it becomes pasty, and finally homogenized by a homogenizer.
The invention also provides a facial mask prepared by the method and capable of removing the acne.
Compared with the prior art, the facial mask capable of removing whelks and the preparation method thereof provided by the invention have the following beneficial effects:
in the preparation method of the mask, the Chinese sage herb, the radix sophorae flavescentis and the rice are used as raw materials, the Chinese sage herb has the main functions of cooling blood, activating blood and diminishing swelling, and the prior art is mainly used for treating traumatic injury. 2g/L citric acid solution is added in the preparation process of the fermented extract of the Chinese sage herb to prevent the oxidation of active substances in the sterilization process and increase the antioxidant activity of the fermented extract of the Chinese sage herb.
The main functions of the sophora flavescens are heat and humidity clearing, insect killing and bacteriostasis, active substances in fresh sophora flavescens slices are dissolved out by using lactic acid bacteria and a 100-200 MPa high-pressure treatment mode, plant cells can be damaged by 100-200 MPa high-pressure short-time treatment, and plant cells are damaged and bacteriostasis are realized by using lactic acid bacteria metabolites in bacteria liquid through low-temperature soaking treatment of the lactic acid bacteria liquid.
Mixing rice flour with radix Sophorae Flavescentis processed residue and organic acid, soaking, performing enzymolysis with acidic protease to hydrolyze protein in rice into absorbable small molecular polypeptide, performing acidolysis and enzymolysis on radix Sophorae Flavescentis residue, and further extracting effective components. The rice extract has the main functions of removing facial stains, resisting oxidation of grease and protecting skin, and can also be used as a carrier of other active ingredients of the extract.
In the last step of the preparation method, after the evening primrose fermentation extract, the sophora flavescens extract and the rice extract are mixed, all the substances can be homogenized and stabilized through high pressure and refrigeration treatment.
The practical application effect of the facial mask proves that the facial mask prepared by the method has good anti-oxidation capabilities of inhibiting bacteria, removing grease and removing free radicals, can remove acne, has obvious effect, and does not have uncomfortable side effects such as irritation or red, swollen and swollen face and the like.
Detailed Description
The present invention will be described in detail with reference to specific embodiments, but it should be understood that the scope of the present invention is not limited by the specific embodiments. The test methods not specified in the following examples are generally conducted under conventional conditions, and the sources of the test materials not specified are commercially available, and the steps thereof will not be described in detail since they do not relate to the invention. If not otherwise indicated, the reaction is carried out at room temperature. The high pressure vessel used in the embodiments of the present invention is commercially available from Jinan Si Ming Tech technologies, Inc.
The invention provides a preparation method of a facial mask capable of removing whelks, which comprises the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Adding lactobacillus liquid into fresh whole plant of the Chinese sage herb, fermenting for 24-36 h at 30 +/-1 ℃, then adding 2g/L citric acid solution, sterilizing, filtering, and collecting filtrate, namely the Chinese sage herb fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus bacterial liquid and the citric acid solution is 10-30: 1: 50-100;
taking fresh radix sophorae flavescentis, cutting into slices, placing the slices in lactobacillus liquid, soaking for 10-12 h at 4 ℃, then treating for 2-3 min under the condition of 100-200 MPa, filtering, respectively collecting filter residues and filtrate, and sterilizing the collected filtrate to obtain a radix sophorae flavescentis extract;
s2, Rice extract
Grinding rice into powder, sieving to obtain rice powder, adding an organic acid solution and the filter residue obtained in S1 into the rice powder, uniformly mixing, standing for 1.5-2 h, adding acid protease, performing water bath enzymolysis at 60 ℃ for 30-50 min, and performing water bath enzyme deactivation at 95 ℃ to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 50-100: 100-150: 1;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 30-50 parts of a Chinese sage herb fermented extract, 10-30 parts of a sophora flavescens extract and 500-1000 parts of a rice extract;
mixing the weighed evening primrose fermentation extract, the sophora flavescens extract and the rice extract, concentrating the obtained mixture to be pasty, treating for 1-3 min under 100-200 MPa, and refrigerating for 4-6 h at 4 ℃ to obtain the mask capable of removing the acne.
The pasty facial mask prepared by the method simplifies the raw material formula of the facial mask, adopts pure natural plants, has no irritation and side effects, is suitable for various skins such as dry, oily and sensitive skins, is directly coated on the face, is applied once every 2d for 15-20 min every time, is washed by clear water, is convenient to use, and has good effects of protecting skin, inhibiting bacteria and removing whelks. The curative effect is better when the composition is stored in a cold storage way and is restored to the room temperature when in use.
The following examples are specifically included.
Example 1
A preparation method of a facial mask capable of removing whelks comprises the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Grinding fresh whole plant of herba Equiseti Ramosissimi into slurry, dissolving out juice of herba Equiseti Ramosissimi sufficiently, adding lactobacillus bacteria solution, fermenting at 30 + -1 deg.C for 24 hr, adding 2g/L citric acid solution, boiling and sterilizing for 15min, filtering with four layers of medical gauze, and collecting filtrate to obtain herba Equiseti Ramosissimi fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactic acid bacteria liquid and the citric acid solution is 10:1: 50;
cutting fresh radix Sophorae Flavescentis into 2mm thick slices, soaking in lactobacillus liquid at 4 deg.C for 10 hr, allowing the lactobacillus liquid to permeate the slice surface, treating under 100MPa for 3min, filtering with two layers of medical gauze, respectively collecting residue and filtrate, boiling the filtrate, and sterilizing for 15min to obtain radix Sophorae Flavescentis extract;
s2, Rice extract
Grinding rice (polished round-grained rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding an organic acid solution and the filter residue obtained in S1 (the filter residue collected in the preparation of the sophora flavescens extract) into the rice powder, uniformly mixing, standing at room temperature, soaking for 1.5h, adding acid protease, performing enzymolysis in a water bath at 60 ℃ for 50min, stirring during enzymolysis, and inactivating the enzyme in the water bath at 95 ℃ for 10min to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 50:100: 1; the organic acid solution is 0.01mol/L acetic acid solution;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 30 parts of Chinese sage herb fermentation extract, 10 parts of sophora flavescens extract and 1000 parts of rice extract;
mixing the weighed herba Equiseti Arvinsis fermented extract, radix Sophorae Flavescentis extract and rice extract, concentrating the obtained mixture to paste, treating at 100MPa for 3min, and refrigerating at 4 deg.C for 6 hr to obtain facial mask for removing acne. The specific operation mode of concentrating into paste is as follows: the mixture obtained in S3 is continuously stirred and heated to evaporate water until no water drops on the surface of the mixture and the mixture becomes pasty, and finally the mixture is homogenized by a homogenizer.
In example 1, the lactic acid bacteria are Lactobacillus plantarum ATCC 14917, and the viable count of the lactic acid bacteria liquid is not less than 106One per ml. Lactobacillus plantarum ATCC 14917 has a good antihypertensive effect and is a highly safe strain for food (see patent publication CN 105062944B). In each step of example 1, the preparation method of the lactic acid bacteria liquid is as follows: activating Lactobacillus plantarum ATCC 14917 seed, inoculating into culture medium, culturing at 30 + -1 deg.C until the viable count in the bacterial liquid is not less than 106Per ml; the culture medium is prepared according to the following method: 50ml of cow milk, 20g of cane sugar, 5g of evening primrose, 5g of fresh radix sophorae flavescentis slices and distilled water are added to a constant volume of 1L, the culture medium is sterilized in a mode of sterilizing the culture medium at 121 ℃ for 15min for later use. In addition, the amount of the additive is determined according to the actual amount of the additiveA step-by-step amplification culture mode is adopted, the inoculation amount is 5-10% during each amplification culture, the amplification culture time is 2d, and the last culture is carried out until the number of viable bacteria in the bacterial liquid is more than or equal to 106The dosage is only one/ml.
Example 2
A preparation method of a facial mask capable of removing whelks comprises the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Grinding fresh whole plant of herba Equiseti Ramosissimi into slurry, dissolving out juice of herba Equiseti Ramosissimi sufficiently, adding lactobacillus bacteria solution, fermenting at 30 + -1 deg.C for 36 hr, adding 2g/L citric acid solution, boiling and sterilizing for 15min, filtering for four layers, and collecting filtrate to obtain herba Equiseti Ramosissimi fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus bacterial liquid and the 2g/L citric acid solution is 30:1: 100;
cutting fresh radix Sophorae Flavescentis into 4mm thick slices, soaking in lactobacillus liquid at 4 deg.C for 12 hr, allowing the lactobacillus liquid to permeate the slice surface, treating under 200MPa for 2min, filtering for two layers, collecting residue and filtrate, boiling and sterilizing the filtrate for 15min to obtain radix Sophorae Flavescentis extract;
s2, Rice extract
Grinding rice (brown rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding organic acid solution and the residue obtained in S1 (residue collected during preparation of radix Sophorae Flavescentis extract) into rice powder, mixing, standing at room temperature for soaking for 2h, adding acid protease, performing enzymolysis in 60 deg.C water bath for 30min, stirring during enzymolysis, and inactivating enzyme in 95 deg.C water bath for 10min to obtain rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 100:150: 1; the organic acid solution is 2g/L malic acid solution;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 50 parts of Chinese sage herb fermentation extract, 30 parts of sophora flavescens extract and 500 parts of rice extract;
mixing the weighed herba Equiseti Arvinsis fermented extract, radix Sophorae Flavescentis extract and rice extract, concentrating the obtained mixture to paste, treating at 200MPa for 1min, and refrigerating at 4 deg.C for 4 hr to obtain facial mask for removing acne. The specific operation mode of concentrating to paste, the lactobacillus strain and the preparation method of the liquid are the same
Example 1.
Example 3
A preparation method of a facial mask capable of removing whelks comprises the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Grinding fresh whole plant of herba Equiseti Ramosissimi into slurry, dissolving out juice of herba Equiseti Ramosissimi sufficiently, adding lactobacillus bacteria solution, fermenting at 30 + -1 deg.C for 30 hr, adding 2g/L citric acid solution, boiling and sterilizing for 15min, filtering with plate-and-frame filter, and collecting filtrate to obtain herba Equiseti Ramosissimi fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus liquid and the 2g/L citric acid solution is 20:1: 80;
cutting fresh radix Sophorae Flavescentis into slices with thickness of 3mm, soaking in lactobacillus liquid at 4 deg.C for 11 hr, allowing the lactobacillus liquid to permeate the slice surface, treating at 140MPa for 2min, filtering with plate-and-frame filter, respectively collecting residue and filtrate, boiling and sterilizing the collected filtrate for 15min to obtain radix Sophorae Flavescentis extract;
s2, Rice extract
Grinding rice (glutinous rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding organic acid solution and the filter residue obtained in S1 (the filter residue collected during preparation of the radix sophorae flavescentis extract) into the rice powder, uniformly mixing, soaking at room temperature for 1.5h, adding acid protease, performing enzymolysis in 60 ℃ water bath for 40min, stirring during enzymolysis, and performing enzyme deactivation in 95 ℃ water bath for 10min to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 80:130: 1; the organic acid solution is a mixed solution of 0.01mol/L acetic acid solution and 2g/L malic acid solution according to the volume ratio of 1: 1;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 40 parts of Chinese sage herb fermented extract, 20 parts of sophora flavescens extract and 800 parts of rice extract;
mixing the weighed herba Equiseti Arvinsis fermented extract, radix Sophorae Flavescentis extract and rice extract, concentrating the obtained mixture to paste, treating at 140MPa for 2min, and refrigerating at 4 deg.C for 5 hr to obtain facial mask for removing acne. The specific operation mode of concentrating to paste, the lactobacillus strain and the preparation method of the liquid are the same
Example 1.
Some experimental data are listed below to demonstrate the effect of the present invention. In the following experiments, the method for evaluating the bacteriostatic effect is as follows: investigation of different samples on Staphylococcus aureus (bacterial liquid concentration 1.3X 10)8CFU/ml), the diameter of the bacteriostatic circle is small, the bacteriostatic effect is good for the case with large diameter, and the bacteriostatic effect is poor for the case with small diameter;
the degreasing effect evaluation method is as follows: the test is carried out according to the method for measuring the detergency in the national standard GB/T13174-2008, the following test is mainly carried out by using grease to contaminate a cloth piece, after a sample to be measured is prepared into a washing solution with the same concentration, the detergency of the sample and the commercially available sulfur soap is measured, the detergency ratio P is expressed, and the calculation formula is as follows:
P＝(R11-R12)/(R21-R22) (1)
in the formula (1), R11Reflectance, R, of the washed cloth pieces for the samples to be tested12Reflectance, R, of the cloth piece before washing for the sample to be tested21Is the reflectivity, R, of the cloth piece after the washing of the sulfur soap22Is the reflectivity of the cloth before the sulphur soap is washed.
The evaluation method of the effect of scavenging free radicals is as follows: the hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity and DPPH radical scavenging capacity of the sample are respectively measured, and the measuring method refers to comparison and analysis of the antioxidant capacity of different lactic acid bacteria, Liu Shao Min et al, Chinese Dairy industry, 2015,43 (8): 8:12. Adding ultrapure water with equal mass before measuring the mask sample, homogenizing to obtain suspension, and measuring.
The method for measuring the hydroxyl radical scavenging capacity comprises the following steps: mixing 1ml of 0.75mmol/L phenanthroline, 2ml of PBS (pH7.4), and 1ml of 0.75mmol/L ferrous sulfate solution, adding 1ml of H with mass fraction of 0.12%2O2The solution was then added with 1ml of the sample to be testedThe solution was incubated at 37 ℃ for 90min, and the change in absorbance at 536nm was measured to determine the hydroxyl radical scavenging ability (A)S-AC)/(AB-AC)×100％，ASAs absorbance value of the sample, ACAs the light absorption value of a control group (distilled water replaces the solution of the sample to be measured) ABAs blank absorbance value (distilled water instead of H)2O2Solution and sample solution to be tested).
The superoxide anion free radical scavenging ability was determined as follows: in each 1ml of reaction solution, the concentration of PBS with pH7.4 is 20mmol/L, NBT, the concentration is 50 mu mol/L, the concentration of NADH is 75 mu mol/L, PMS, the concentration is 15 mu mol/L, and the volume of a sample solution to be detected is 50 mu L; incubating at 37 deg.C for 5min, and measuring change of absorbance at 560nm to obtain superoxide anion free radical scavenging ability (A)S-AC)/AS×100％，ASAs absorbance value of the sample, ACControl absorbance (distilled water instead of sample).
The DPPH free radical scavenging ability is determined as follows: dissolving DPPH in methanol to obtain 0.1mmol/L solution, mixing 2ml of the solution and 1ml of the sample solution to be measured, incubating at room temperature in a dark place for 30min, measuring the change of light absorption value at 517nm, and determining DPPH free radical scavenging capacity (A)S-AC)/AS×100％，ASAs absorbance value of the sample, ACControl absorbance (distilled water instead of sample).
Bacteriostasis, degreasing and free radical removing effects of evening primrose in one or different treatment modes
Processing method a 1: the fermented extract of evening primrose was prepared in the same manner as in example 1.
Processing method a 2: grinding whole plant of herba Equiseti Ramosissimi into slurry, dissolving out juice of herba Equiseti Ramosissimi sufficiently, leaching with distilled water for 24 hr, adding distilled water for the second time, boiling and sterilizing for 15min, filtering with four layers of medical gauze, and collecting filtrate to obtain herba Equiseti Ramosissimi fermented extract; the mass ratio of the whole plant of the Chinese sage herb to the distilled water is 10: 51.
Processing method a 3: grinding whole plant of herba Equiseti Ramosissimi into slurry, dissolving juice in herba Equiseti Ramosissimi sufficiently, adding lactobacillus bacteria solution, fermenting at 30 + -1 deg.C for 24 hr, adding distilled water, boiling for sterilizing for 15min, filtering with four layers of medical gauze, and collecting filtrate to obtain herba Equiseti Ramosissimi fermented extract; the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus liquid and the distilled water is 10:1: 50.
The bacteriostatic, degreasing and free radical scavenging effects of the evening primrose in different treatment modes are shown in table 1. As is clear from the results in Table 1, the treatment method A1 exhibited the best bacteriostatic, degreasing and radical scavenging effects.
TABLE 1 bacteriostatic, degreasing and free radical scavenging effects of the fermented extract of Thalassiosira
Second, the bacteriostatic effect of sophora flavescens with different treatment modes
Processing method B1: the sophora flavescens extract was prepared in the manner of reference example 1.
Processing method B2: cutting fresh radix Sophorae Flavescentis into 2mm thick slices, soaking in distilled water at 4 deg.C for 10 hr, allowing lactobacillus bacteria solution to permeate the surface of the slices, filtering with two layers of medical gauze, respectively collecting residue and filtrate, boiling the filtrate, and sterilizing for 15min to obtain radix Sophorae Flavescentis extract.
Processing method B3: cutting fresh radix Sophorae Flavescentis into 2mm thick slices, soaking in lactobacillus liquid at 4 deg.C for 10 hr, allowing the lactobacillus liquid to permeate the slice surface, filtering with two layers of medical gauze, respectively collecting residue and filtrate, boiling the filtrate, and sterilizing for 15min to obtain radix Sophorae Flavescentis extract.
The bacteriostatic effect of sophora flavescens is shown in table 2. From the results in Table 2, it is clear that the treatment method B1 is the most excellent in bacteriostasis.
TABLE 2 bacteriostatic effect of Sophora flavescens Aiton extract
| Processing method B1 | Processing method B2 | Processing method B3 | |
| Diameter of zone of inhibition | 23.1±0.02mm | 15.7±0.01mm | 17.3±0.01mm |
Degreasing and free radical removing effects of rice extract with different treatment modes
Processing method C1: a rice extract was prepared in the same manner as in example 1.
Processing method C2: grinding rice (polished round-grained rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding distilled water into the rice powder, mixing uniformly, and soaking at room temperature for 1.5h to obtain a rice extract; wherein the mass ratio of the rice flour to the distilled water is 50: 100.
Processing method C3: grinding rice (polished round-grained rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding distilled water and the filter residue obtained in S1 (the filter residue collected in the preparation of the sophora flavescens extract) into the rice powder, uniformly mixing, and soaking at room temperature for 1.5h to obtain a rice extract; wherein the mass ratio of the rice flour, the distilled water and the filter residue obtained in the S1 is 50:100: 1.
Processing method C4: grinding rice (polished round-grained rice) into powder, sieving with a 200-mesh sieve to obtain rice powder, adding organic acid solution and the filter residue obtained in S1 (the filter residue collected in the preparation of the radix sophorae flavescentis extract) into the rice powder, uniformly mixing, and soaking at room temperature for 1.5h to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 50:100: 1; the organic acid solution is 0.01mol/L acetic acid solution.
The degreasing and radical scavenging effects of the rice extracts of the different treatment modes are shown in Table 3, and from the results of Table 3, it can be seen that the degreasing and radical scavenging effects of treatment mode C1 are the best.
TABLE 3 bacteriostasis and degreasing of rice extract
Fourthly, the facial mask has the effects of inhibiting bacteria, removing grease and removing free radicals
The effects of bacteriostasis, degreasing and free radical scavenging of the facial masks of the embodiments 1-3 of the invention are shown in Table 4,
TABLE 4 bacteriostatic, degreasing and free radical scavenging effects of the masks of examples 1-3
Fifthly, the practical application effect of the mask of the invention
Randomly selecting 100 acne patients of 13-28 years of age who voluntarily participate in the test, wherein 25 patients with severe symptoms (manifested as red and swollen face, pimple area occupying about 1/3), 35 patients with moderate symptoms (manifested as red and swollen face, pimple area occupying about 1/6), and 40 patients with mild symptoms (manifested as red and swollen face, pimple area occupying about 1/10); 20 subjects with acne, pimples of age 13-28 years who were voluntarily enrolled in the test were randomly selected. The facial mask of example 1 was applied directly to the face once every 2 days for 20min, and then the face was washed with clean water and observed for efficacy after 8 consecutive uses.
The criteria for the therapeutic effect are as follows: (1) and (3) healing: the red and swollen face disappears, no pimple exists, no scar exists, and the skin is glossy; (2) the method is remarkably effective: the red swelling of the face disappears, a little keloid and a little scar; (3) the method has the following advantages: the red and swollen face disappears, few keloids and scars are generated, and the skin is glossy; (4) and (4) invalidation: the symptoms did not improve or worsen. The practical application results are as follows: the symptoms of patients with severe acne are improved effectively, the symptoms of patients with moderate acne are improved effectively, patients with mild acne and patients with acne and pimple are healed, and all patients do not have irritation or uncomfortable side effects such as red, swollen and swollen face. The facial mask prepared by the method can remove the acne and has obvious effect.
Finally, it is noted that the Lactobacillus selected by the invention is Lactobacillus plantarum ATCC 14917 which has good pressure resistance, and the viable count in the Lactobacillus liquid is more than or equal to 106The diameter of the inhibition zone can reach 13.5mm at each ml, the effect is obvious, so the bacteria concentration is not required to be cultured to be more than or equal to 108The culture time of the thalli is saved. The method of example 1 is adopted to prepare the lactobacillus bacterial solution, the culture medium adopts the snow evening primrose and fresh radix sophorae flavescentis slices (the thickness is 3mm) to carry out acclimatization culture on the lactobacillus, so that the lactobacillus bacterial solution is more suitable for a new fermentation environment, the viable count is more than or equal to 106The fermentation time per ml can be good, namely the fermentation time in S1 is only 24-36 h. In contrast, if only cow milk and sucrose are added into the culture medium, the viable count is more than or equal to 108The seed liquid per ml has good fermentation effect on the Chinese sage herb and the lightyellow sophora root, and the fermentation time of the Chinese sage herb is required to be 48 hours.
In the preparation method of the embodiment of the invention, in S1, the sterilization mode is boiling sterilization for 15 min. The reason why autoclaving at 121 ℃ is not used is to prevent the autoclaving environment from destroying the activity of the extracted active substance.
It should be noted that, when referring to a numerical range in the present invention, any numerical value within the numerical range is feasible, and for the sake of avoiding redundancy, only the preferred embodiments of the present invention are described, but once the basic inventive concept is known, other variations and modifications can be made to the embodiments by those skilled in the art. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. A preparation method of a facial mask capable of removing whelks is characterized by comprising the following steps:
s1, preparing the fermented extract of the Chinese sage herb and the extract of the lightyellow sophora root
Adding lactobacillus liquid into fresh whole plant of the Chinese sage herb, fermenting for 24-36 h at 30 +/-1 ℃, then adding 2g/L citric acid solution, sterilizing, filtering, and collecting filtrate, namely the Chinese sage herb fermented extract; wherein the mass ratio of the whole plant of the Chinese sage herb, the lactobacillus bacterial liquid and the citric acid solution is 10-30: 1: 50-100; the Lactobacillus is Lactobacillus plantarum ATCC 14917, and the viable count in the Lactobacillus strain liquid is more than or equal to 106Per ml;
taking fresh radix sophorae flavescentis, cutting into slices, placing the slices in lactobacillus liquid, soaking for 10-12 h at 4 ℃, then treating for 2-3 min under the condition of 100-200 MPa, filtering, respectively collecting filter residues and filtrate, and sterilizing the collected filtrate to obtain a radix sophorae flavescentis extract;
s2, Rice extract
Grinding rice into powder, sieving to obtain rice powder, adding an organic acid solution and the filter residue obtained in S1 into the rice powder, uniformly mixing, standing for 1.5-2 h, adding acid protease, performing water bath enzymolysis at 60 ℃ for 30-50 min, and performing water bath enzyme deactivation at 95 ℃ to obtain a rice extract; wherein the mass ratio of the rice flour, the organic acid solution and the filter residue obtained in S1 is 50-100: 100-150: 1;
s3, preparing the facial mask capable of removing whelks
Weighing the following components in parts by weight: 30-50 parts of a Chinese sage herb fermented extract, 10-30 parts of a sophora flavescens extract and 500-1000 parts of a rice extract;
mixing the weighed evening primrose fermentation extract, the sophora flavescens extract and the rice extract, concentrating the obtained mixture to be pasty, treating for 1-3 min under 100-200 MPa, and refrigerating for 4-6 h at 4 ℃ to obtain the mask capable of removing the acne.
2. The method for preparing the mask for removing whelk according to claim 1, wherein the method for preparing the lactobacillus bacterial liquid comprises the following steps: activated lactic acid bacteria LactobaThe strain of the strain Cillus plantarum ATCC 14917 is inoculated into a culture medium and cultured at the temperature of 30 +/-1 ℃ until the viable count in the strain liquid is more than or equal to 106Per ml; the culture medium is prepared according to the following method: 50ml of cow milk, 20g of cane sugar, 5g of evening primrose, 5g of fresh radix sophorae flavescentis slices and distilled water are added to a constant volume of 1L, and a culture medium is sterilized for later use.
3. The method for preparing a facial mask for removing acne as claimed in claim 2, wherein the culture medium is sterilized by 121 ℃ for 15min when preparing the lactobacillus bacterial solution.
4. The method of preparing a facial mask for removing acne according to claim 1, wherein in S2, the organic acid solution is 0.01mol/L acetic acid solution, 2g/L malic acid solution or a mixture thereof at a volume ratio of 1: 1.
5. The method for preparing a facial mask for removing pimples according to claim 1, wherein the sterilization means is boiling sterilization for 15min at S1.
6. The method for preparing a facial mask for removing pimples according to claim 1, wherein the concentration to a pasty state in S3 is performed in the following manner: the mixture obtained in S3 is continuously stirred and heated to evaporate water until it becomes pasty, and finally homogenized by a homogenizer.
7. The facial mask for removing whelk prepared by the preparation method according to any one of claims 1 to 6.
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