CN111956534B - Skin care composition for treating acne sensitive skin and preparation method thereof - Google Patents
Skin care composition for treating acne sensitive skin and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to a skin care composition for focusing on repairing oil skin sensitive pox muscles, and a preparation method and application thereof. The paint comprises the following components in parts by weight: 20-45 parts of yeast fermentation filtrate of the Australian orange extract, 10-22 parts of rosa roxburghii tratt extract and 3-10 parts of arachnoid wildrye extract. The skin care composition takes the yeast fermentation filtrate of the extract of the orange australis as a main functional component, and the Rosa roxburghii water extract and the 60-92% ethanol extract of the larch are compounded to play roles of effectively removing acnes and avoiding skin anaphylactic reaction.
Description
Technical Field
The invention belongs to the technical field of cosmetics. More particularly, it relates to a skin care composition that focuses on repairing oil skin sensitive vaccinia muscle and a method of making the same.
Background
The acne is commonly called as whelk, pimple and acne, and has a plurality of induction factors, which are mainly caused by that male hormone secretion promotes sebaceous gland to secrete more grease, so that hair follicle and sebaceous gland are blocked and inflamed. In various skin types, the pox repair on the sensitive skin of the greasy skin is the most difficult to maintain, and the skin is mainly formed by the following reasons: the facial mask has the advantages that frequent water supplement or excessive use of products with high oil content causes excessive hydration of facial skin, even causes inflammation and aggravates acne; ② excessive use of salicylic acid and tartaric acid leads to the skin cuticle self-repair speed not reaching the metabolism speed, and causes the skin barrier function to be damaged. Aiming at the skin, the skin is required to be prevented from causing sensitive reaction while controlling oil and reducing grease secretion, but most products in the market at present aim at oiled skin pox muscles, the products aiming at oiled skin sensitive pox muscles are fewer, and if the products are used for the oiled skin sensitive pox muscles, the sensitive skin sensitive reaction is easily stimulated, so that the skin inflammation causes secondary damage to the skin. Therefore, there is a need to provide an anti-acne product suitable for use on the acne sensitive vaccinia muscle.
Disclosure of Invention
The invention aims to overcome the defects of the existing acne removing product and provide an acne removing product suitable for oilskin sensitive acne muscles, which can effectively remove acnes and does not cause skin allergic reaction.
The above purpose of the invention is realized by the following technical scheme: a skin care composition for reducing acne skin sensitivity comprises the following components in parts by weight:
20-45 parts of yeast fermentation filtrate of the extract of Australia finger orange,
-Rosa roxburghii extract 10-22 parts, and
3-10 parts of a arachnoid bluegrass extract.
Preferably, the skin care composition comprises the following components in parts by weight:
35 parts of yeast fermentation filtrate of the extract of Australia finger orange,
-Rosa roxburghii extract 16 parts, and
5 parts of a spider-web-like Lancete extract.
Preferably, the skin care composition comprises the following components in parts by weight:
22 parts of yeast fermentation filtrate of the extract of orange in Australia,
-22 parts of rosa roxburghii tratt extract, and
8 parts of a spider-web-like Lancete extract.
Preferably, the skin care composition comprises the following components in parts by weight:
45 parts of yeast fermentation filtrate of the extract of orange in Australia,
-the aqueous extract of Rosa roxburghii 10 parts, and
-3 parts of a spider-web Lancete extract.
Preferably, the skin care composition comprises the following components in parts by weight:
32 parts of yeast fermentation filtrate of the extract of orange in Australia,
-12 parts of an aqueous extract of rosa roxburghii, and
10 parts of a spider-web-like Lancete extract.
Preferably, the preparation of the fermentation filtrate of the orange extract yeast comprises the following steps:
step one, taking pulp of Australian finger oranges as a raw material, crushing the tissue, adding an organic solvent for extraction, and concentrating an extracting solution under reduced pressure to obtain an extract of the Australian finger oranges;
and step two, adding the obtained Australian orange extract into a yeast culture medium according to a certain volume ratio to form a fermentation substrate, inoculating yeast for fermentation to obtain fermentation liquor, and filtering to obtain the Australian orange extract.
Preferably, in the first step, the organic solvent is an ethanol solution, and the volume fraction of the ethanol solution is 40-70%. More preferably, the volume fraction of the ethanol solution is 55%, 65% or 70%; more preferably, the volume fraction of the ethanol solution is 65%.
Preferably, in the first step, the extraction temperature is 75-90 ℃ and the extraction is carried out for 1-3 times. Preferably, the extraction temperature is 75 ℃, 80 ℃, 85 ℃ or 90 ℃; more preferably, the extraction temperature is 85 ℃.
Preferably, in the second step, the volume of the added Australian orange extract is 10-21%. Preferably, australia refers to an orange extract that may be added in a volume of 10%, 15%, 18% or 21%. More preferably, Australia refers to 15% by volume of orange extract.
Preferably, in the second step, the fermentation parameters are: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 25-30 ℃; the fermentation time is 2-5 days. More preferably, the fermentation temperature is 28 ℃; the fermentation time was 3 days.
Preferably, the roxburgh rose extract is a roxburgh rose water extract. It can be prepared by water extraction steps conventional in the art, and more preferably, the preparation steps are:
taking the roxburgh rose fruit, drying, crushing the tissue, adding deionized water in an amount which is 3-6 times that of the roxburgh rose fruit, decocting for 1-3 times, 60-150 min each time, filtering, combining the filtrates, and concentrating under reduced pressure to obtain the roxburgh rose fruit extract.
Preferably, the arachnoid palustris extract is a 60-92% ethanol extract of arachnoid palustris. The preparation method of the 60-92% ethanol extract of the arachnoid nepenthes can adopt an ethanol extraction method adopted in the field, and more preferably comprises the following steps:
taking the roots of the arachnoid nepalensis, cleaning, drying, crushing, adding 2-5 times of 60-92% ethanol solution, performing reflux extraction at 60-80 ℃ for 1-3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain the compound.
The invention also aims to provide essence for concentrating on repairing the oil skin sensitive acne muscle, which comprises 1-99.99 wt% of the skin care composition. More preferably, the skin care composition is 22%, 25%, 32% or 42% by weight of the serum.
More preferably, the essence water may further include other adjuvants commonly used in the cosmetic field, including but not limited to surfactants, thickeners, neutralizers, and the like.
The 'Australia refers to orange extract yeast fermentation filtrate' is rich in bioflavonoids, chlorogenic acid and tartaric acid, wherein the bioflavonoids can inhibit the activity of propionibacterium acnes and key inflammatory enzymes, and have the effects of removing acnes and relieving skin inflammation; the contained fruit acid has the function of mildly accelerating cutin metabolism, and the skin is smoother and brighter after the application; the synergistic Rosa roxburghii water extract and the 60-92% ethanol extract of the arachnoid agrimony can effectively repair acnes, do not cause allergic reaction of skin, can continuously help the skin to lock water, have strong repair and defense capabilities, and achieve the synergistic effect by combining the Rosa roxburghii water extract and the arachnoid agrimony.
The invention has the following beneficial effects:
the skin care composition takes the yeast fermentation filtrate of the extract of the orange australis as a main functional component, and the Rosa roxburghii water extract and the 60-92% ethanol extract of the larch are compounded to play roles of effectively removing acnes and avoiding skin anaphylactic reaction.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
The yeast culture medium comprises the following components: each 100ml of water contains 18g of potato, 5g of glucose and 3g of agar.
Example 1 skin care composition
The skin care composition comprises 35 parts of yeast fermentation filtrate of an orange extract of Australia, 16 parts of a rosa roxburghii water extract and 5 parts of an arachnoid wildrye extract.
Preparing a yeast fermentation filtrate of the Macadimia indica extract:
step one, taking pulp of the Australian finger orange, cleaning, crushing the tissue, sieving by a 50-mesh sieve, adding 5 times of 65% ethanol solution, carrying out reflux extraction at 85 ℃ for 3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain an Australian finger orange extract;
step two, adding the prepared Australian finger orange extract into a yeast culture medium according to the volume ratio of 15%, uniformly mixing, and sterilizing at 121 ℃ to obtain a fermentation substrate;
step three, inoculating saccharomycetes which are cultured in a shaking table in advance into the fermentation substrate to ensure that the concentration of the saccharomycetes in the fermentation substrate is 8 multiplied by 106cfu/ml, fermenting, and filtering the fermentation liquor by an ultrafiltration membrane after the fermentation is finished to obtain the product; wherein the fermentation parameters are as follows: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 28 ℃; the fermentation time was 3 days.
Preparing the rosa roxburghii water extract:
collecting fructus Rosae Normalis, drying, pulverizing tissue, adding 5 times of deionized water, decocting for 2 times (120 min each time), filtering, mixing filtrates, and concentrating under reduced pressure.
The preparation method of the arachnoid bluegrass extract comprises the following steps:
cleaning root of herba Adenophorae Trachelioidis, drying, pulverizing, adding 4 times of 85% ethanol solution, reflux-extracting at 75 deg.C for 3 times, filtering, mixing filtrates, and concentrating under reduced pressure.
Example 2 skin care composition
The skin care composition comprises 22 parts of yeast fermentation filtrate of an orange extract of Australia, 22 parts of a rosa roxburghii water extract and 8 parts of an arachnoid wildrye extract.
Preparing a yeast fermentation filtrate of the Macadimia indica extract:
step one, taking pulp of the Australian finger orange, cleaning, crushing the tissue, sieving by a 50-mesh sieve, adding 6 times of 55% ethanol solution, carrying out reflux extraction at 80 ℃ for 3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain an Australian finger orange extract;
step two, adding the prepared Australian finger orange extract into a yeast culture medium according to the volume ratio of 10%, uniformly mixing, and sterilizing at 121 ℃ to obtain a fermentation substrate;
step three, inoculating saccharomycetes which are cultured in a shaking table in advance into the fermentation substrate to ensure that the concentration of the saccharomycetes in the fermentation substrate is 8 multiplied by 106cfu/ml, fermenting, and filtering the fermentation liquor by an ultrafiltration membrane after the fermentation is finished to obtain the product; wherein the fermentation parameters are as follows: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 28 ℃; the fermentation time was 3 days.
Preparing the rosa roxburghii water extract:
collecting fructus Rosae Normalis, drying, pulverizing, adding 6 times of deionized water, decocting for 3 times (150 min each time), filtering, mixing filtrates, and concentrating under reduced pressure.
The preparation method of the arachnoid bluegrass extract comprises the following steps:
cleaning root of herba Adenophorae Trachelioidis, drying, pulverizing, adding 5 times of 75% ethanol solution, reflux-extracting at 80 deg.C for 2 times, filtering, mixing filtrates, and concentrating under reduced pressure.
Example 3 skin care composition
The skin care composition comprises 45 parts of yeast fermentation filtrate of an orange extract of Australia, 10 parts of a rosa roxburghii water extract and 3 parts of a subarachnoid auricularia extract.
Preparing a yeast fermentation filtrate of the Macadimia indica extract:
step one, taking pulp of the Australian finger orange, cleaning, crushing the tissue, sieving by a 50-mesh sieve, adding 4 times of 70% ethanol solution, carrying out reflux extraction at 90 ℃ for 2 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain an Australian finger orange extract;
step two, adding the prepared Australian finger orange extract into a yeast culture medium according to the volume ratio of 21%, uniformly mixing, and sterilizing at 121 ℃ to obtain a fermentation substrate;
step three, inoculating saccharomycetes which are cultured in a shaking table in advance into the fermentation substrate to ensure that the concentration of the saccharomycetes in the fermentation substrate is 8 multiplied by 106cfu/ml, fermenting, and filtering the fermentation liquor by an ultrafiltration membrane after the fermentation is finished to obtain the product; wherein the fermentation parameters are as follows: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 30 ℃; the fermentation time was 5 days.
Preparing the rosa roxburghii water extract:
collecting fructus Rosae Normalis, drying, pulverizing tissue, adding 3 times of deionized water, decocting for 2 times (100 min each time), filtering, mixing filtrates, and concentrating under reduced pressure.
The preparation method of the arachnoid bluegrass extract comprises the following steps:
cleaning root of herba Adenophorae Trachelioidis, drying, pulverizing, adding 6 times of 92% ethanol solution, extracting at 65 deg.C under reflux for 3 times, filtering, mixing filtrates, and concentrating under reduced pressure.
Example 4 skin care composition
The skin care composition comprises 32 parts of yeast fermentation filtrate of an orange extract of Australia, 12 parts of a rosa roxburghii water extract and 10 parts of an arachnoid wildrye extract.
Preparing a yeast fermentation filtrate of the Macadimia indica extract:
step one, taking pulp of the Australian finger orange, cleaning, crushing the tissue, sieving by a 50-mesh sieve, adding 5 times of 50% ethanol solution, carrying out reflux extraction at 75 ℃ for 3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain an Australian finger orange extract;
step two, adding the prepared Australian finger orange extract into a yeast culture medium according to the volume ratio of 18%, uniformly mixing, and sterilizing at 121 ℃ to obtain a fermentation substrate;
step three, inoculating saccharomycetes which are cultured in a shaking table in advance into the fermentation substrate to ensure that the concentration of the saccharomycetes in the fermentation substrate is 8 multiplied by 106cfu/ml, fermenting, and filtering the fermentation liquor by an ultrafiltration membrane after the fermentation is finished to obtain the product; wherein the fermentation parameters are as follows: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 28 ℃; the fermentation time was 3 days.
Preparing the rosa roxburghii water extract:
collecting fructus Rosae Normalis, drying, pulverizing tissue, adding 5 times of deionized water, decocting for 2 times (120 min each time), filtering, mixing filtrates, and concentrating under reduced pressure.
The preparation method of the arachnoid bluegrass extract comprises the following steps:
cleaning root of herba Adenophorae Trachelioidis, drying, pulverizing, adding 4 times of 85% ethanol solution, reflux-extracting at 75 deg.C for 3 times, filtering, mixing filtrates, and concentrating under reduced pressure.
Example 5~ 8 skin care essence formula (mass fraction)
| Components | Example 5 | Example 6 | Example 7 | Example 8 |
| 1, 2-pentanediol | 3％ | 3％ | 3％ | 3％ |
| Butanediol | 1％ | 1％ | 1％ | 1％ |
| Tridecyl alcohol polyether-9 | 3％ | 3％ | 3％ | 3％ |
| Skin care compositions | 32％ | 25％ | 22％ | 42％ |
| Sodium benzoate | 0.1％ | 0.1％ | 0.1％ | 0.1％ |
| Sorbic acid | 0.1％ | 0.1％ | 0.1％ | 0.1％ |
| Deionized water | Balance of | Balance of | Balance of | Balance of |
The preparation method comprises the following steps:
adding deionized water into a stirring pot, heating to 42 deg.C, slowly adding 1, 2-pentanediol, butanediol, tridecyl alcohol polyether-9 and skin care composition while stirring, stirring well, and keeping the temperature for 5 min; cooling to 35 deg.C, adding sodium benzoate and sorbic acid, stirring, inspecting, discharging, and packaging.
Comparative example 1 differs from example 5 in that the skin care composition is only australian orange extract yeast fermentation filtrate, with the remaining parameters being the same as in example 5.
Comparative example 2, which differs from example 5 in that the skin care composition is only an aqueous extract of rosa roxburghii tratt, the remaining parameters are the same as in example 5.
Comparative example 3 differs from example 5 in that the skin care composition is only an ethanol extract of arachnoid bluegrass, and the remaining parameters are the same as in example 5.
Comparative example 4 differs from example 5 in that the yeast fermentation filtrate of the Australian finger orange extract was replaced by the Australian finger orange extract, and the other parameters were the same as in example 5.
The preparation method of the Australia orange extract refers to the step one in the example 1.
Comparative example 5 differs from example 5 in that the yeast fermentation filtrate of the Australian finger orange extract was replaced by the lactic acid bacteria fermentation filtrate of the Australian finger orange extract, and the other parameters were the same as in example 5.
Wherein, the lactobacillus fermentation parameters are as follows: the stirring speed is 150-200 rpm; the ventilation volume is 1-3 VVM; the fermentation temperature is 37 ℃; the fermentation time was 3 days. Wherein the lactic acid bacteria culture medium comprises the following components: per 1000ml of water: 220g of potatoes and 10-15 g of agar, and the pH is natural.
Test example I, efficacy test
1.1 test sample: the essence water of examples 5 to 8 and comparative examples 1 to 5 was used as a test sample
1.2 test methods: 90 female volunteers are selected, the age is 22-32 years old, the skin characteristics of oil skin and sensitive muscle are mild and moderate acne patients, the patients are randomly divided into 9 groups, and 10 cases are selected. The test samples were administered to each group of subjects daily for 28 consecutive days.
1.3 index determination:
1.3.1 oil-controlling acne-removing effect determination: the subjects' facial condition before and at day 28 of use were evaluated clinically using the GAGS acne scoring system, with the evaluation criteria shown in Table 1 below and the test results shown in Table 2 below;
1.3.2 skin barrier function assay: the transdermal skin water loss (g/hm) of each group of subjects before and 28 days after use was measured using a DERMALAB tester from cottex technical Aps, Denmark2) The continuous measurement method was used for each measurement, the reading period was 1 second, the average of 60 data was measured for each measurement of 1 minute, and the change rate of the skin transdermal water loss rate on day 28 compared to that before use was calculated, and the test results are shown in table 3 below.
1.3.3 skin allergy assay: the Mexameter test instrument was used to measure the skin red pigment content of each group of subjects before and 28 days after use, and the rate of change of skin transdermal red pigment on day 28 compared to before use was calculated, with the test results shown in Table 4 below; the VISA skin tester was used to record the photographs of the red blood streak on the face before and 28 days after use, and the change in red blood streak was evaluated, and the rate of change in red blood streak on day 28 compared to that before use was calculated, with the evaluation criteria shown below and the evaluation results shown in table 5 below.
Evaluation criteria: more than 30% of red blood streak is removed as obvious effect; 5-30% of red blood streak is effectively removed; less than 5% of red blood streak removed or not significantly ineffective.
Each index test environment: and (3) measuring the test result indoors at the temperature of 22-25 ℃ and the relative humidity of 35-45%, and starting the test after each group of test subjects enter a test room for rest for 30 minutes.
2. Results
TABLE 1 GAGS acne System Scoring Standard
| Total score | 1 to 13 minutes | 14 to 21 minutes | 22 to 26 minutes | 27 to 31 minutes |
| Rank of | Mild degree of | Of moderate degree | Severe degree | Is very serious |
TABLE 2 Change in GAGS score in the groups of subjects
| Test sample | Before use | Day 28 |
| Example 5 | 18.65±3.12 | 8.28±1.45** |
| Example 6 | 20.37±4.39 | 12.51±2.57** |
| Example 7 | 16.28±2.67 | 6.63±1.02** |
| Example 8 | 17.54±3.15 | 7.81±2.36** |
| Comparative example 1 | 15.69±2.14 | 10.62±1.44* |
| Comparative example 2 | 20.18±5.63 | 18.37±3.75 |
| Comparative example 3 | 19.54±3.85 | 16.45±3.82 |
| Comparative example 4 | 17.55±4.92 | 11.17±2.56* |
| Comparative example 5 | 18.39±3.37 | 12.21±2.69* |
Note: compared with the traditional Chinese medicine preparation before use,*P＜0.05；**P＜0.01。
as can be seen from Table 1, the combination of the yeast fermentation filtrate of the Australian finger orange extract, the rosa roxburghii water extract and the arachnoid grass extract has a good effect of treating acne, and the score of the acne is very obvious different (P is less than 0.01) after 28 days of use compared with that before use; analysis of other comparative examples shows that, if lactic acid bacteria are used for replacing yeast for fermentation and the fermentation filtrate of the orange extract yeast of Australia is used alone for 28 days, the acne score is remarkably reduced, and the result shows that the fermentation filtrate of the orange extract yeast of Australia has a good effect on treating acne; but the single use of the roxburgh rose fruit water extract or the arachnoid grass extract has no obvious improvement effect on the acne.
TABLE 3 TWEL changes in facial skin of subjects in each group
| Test sample | Before use | Day 28 | Rate of change |
| Example 5 | 20.15 | 11.63 | -42.28％ |
| Example 6 | 18.36 | 10.87 | -40.80％ |
| Example 7 | 21.24 | 12.79 | -39.78％ |
| Example 8 | 20.68 | 11.51 | -44.34％ |
| Comparative example 1 | 22.65 | 26.54 | +17.17％ |
| Comparative example 2 | 17.57 | 13.52 | -23.06％ |
| Comparative example 3 | 19.25 | 13.06 | -32.16％ |
| Comparative example 4 | 21.33 | 22.15 | +3.84％ |
| Comparative example 5 | 20.58 | 15.48 | -24.78％ |
Note: rate of change ═ skin TEWL value-pre-use TEWL value on day 28)/pre-use TEWL value × 100%
As can be seen from the above table, the TWEL value of the skin of each subject was 17g/hm before use2The above shows that the skin barrier is damaged, and the results show that the combination of the orange extract, the yeast fermentation filtrate, the rosa roxburghii water extract and the arachnoid grass extract has a good effect of repairing the damaged skin barrier, the TWEL value of the skin is reduced by more than 40 percent after the skin is used for 28 days, and the TWEL value is 10-12 g/hm2Meanwhile, the skin barrier is restored to be normal after the skin care essence water of the examples 5-8 is used; the acne removal of the yeast fermentation filtrate of the Australian finger orange extract is enhanced by singly using the yeast fermentation filtrate of the Australian finger orange extract or replacing the yeast fermentation filtrate of the Australian finger orange extract with the extract of the Australian finger orange, which is probably related to the contained tartaric acid and chlorogenic acid.
TABLE 4 changes in facial skin haematochrome in the subjects of each group
TABLE 5 Change in Red blood streak in the Subjects of each group
| Sample (I) | Total number of cases | Number of cases/effects | Effective/number of cases | Number of invalid/instances |
| Example 5 | 10 | 8 | 2 | 0 |
| Example 6 | 10 | 7 | 3 | 0 |
| Example 7 | 10 | 8 | 2 | 0 |
| Example 8 | 10 | 8 | 2 | 0 |
| Comparative example 1 | 10 | 0 | 0 | 10 |
| Comparative example 2 | 10 | 1 | 4 | 5 |
| Comparative example 3 | 10 | 1 | 3 | 6 |
| Comparative example 4 | 10 | 1 | 2 | 7 |
| Comparative example 5 | 10 | 2 | 3 | 5 |
From the above tables 4 and 5, it can be seen that the combination of the yeast fermentation filtrate of the Australian finger orange extract, the rosa roxburghii water extract and the arachnoid grass extract is used as an acne removing product, so that the acne can be effectively removed, and the allergic reaction of the skin can not be caused or even reduced; the results of the tests in the tables show that although the yeast fermentation filtrate of the Australian orange extract or the Australian orange extract has a certain acne removing effect, the yeast fermentation filtrate or the Australian orange extract has a large stimulation effect on skin due to the fact that the yeast fermentation filtrate or the Australian orange extract contains more tartaric acid and chlorogenic acid, and after the yeast fermentation filtrate or the Australian orange extract is used, the skin is damaged and allergic reaction is aggravated, and the yeast fermentation filtrate or the Australian orange extract is not.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (6)
1. A skin care composition for repairing the acne sensitive acne muscle is characterized by comprising the following components in parts by weight:
20-45 parts of yeast fermentation filtrate of the extract of Australia finger orange,
-Rosa roxburghii extract 10-22 parts, and
-3-10 parts of a arachnoid bluegrass extract;
the preparation method of the fermentation filtrate of the orange extract yeast comprises the following steps:
step one, taking pulp of Australian finger oranges as a raw material, crushing the tissue, adding an organic solvent for extraction, and concentrating an extracting solution under reduced pressure to obtain an extract of the Australian finger oranges;
step two, adding the obtained Australian orange extract into a yeast culture medium according to a certain volume ratio to form a fermentation substrate, inoculating yeast for fermentation to obtain fermentation liquor, and filtering to obtain the extract;
the roxburgh rose extract is a roxburgh rose water extract; the preparation steps of the rosa roxburghii tratt water extract are as follows: taking Rosa roxburghii fruit, drying, crushing tissue, adding 3-6 times of deionized water, decocting for 1-3 times (60-150 min each time), filtering, combining filtrates, and concentrating under reduced pressure to obtain the final product;
the arachnoid palum extract is a 60-92% ethanol extract of arachnoid palum; the preparation method of the arachnoid nepenthes extract comprises the following steps: taking the roots of the arachnoid nepalensis, cleaning, drying, crushing, adding 2-5 times of 60-92% ethanol solution, performing reflux extraction at 60-80 ℃ for 1-3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain the compound.
2. The skin care composition according to claim 1, comprising the following components in parts by weight:
35 parts of yeast fermentation filtrate of the extract of Australia finger orange,
-Rosa roxburghii extract 16 parts, and
5 parts of a spider-web-like Lancete extract.
3. The skin care composition according to claim 1, wherein in the first step, the organic solvent is an ethanol solution with a volume fraction of 40-70%.
4. The skin care composition according to claim 1, wherein in the first step, the extraction temperature is 75 to 90 ℃ and the extraction is performed 1 to 3 times.
5. The skin care composition of claim 1, wherein in step two, the volume of the added Australian orange extract is 10-21%.
6. The skin care composition of claim 1, wherein in step two, the fermentation parameters are: the pH value is 4.3-5.6; the stirring speed is 100-200 rpm; the ventilation volume is 1-4 VVM; the fermentation temperature is 25-30 ℃; the fermentation time is 2-5 days.
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| JP2010531816A (en) * | 2007-07-04 | 2010-09-30 | ベイジン ギンコ グループ | Cosmetic composition containing bilberry extract and its application |
| CN110419696A (en) * | 2019-06-28 | 2019-11-08 | 贵州大学 | A kind of Rosa roxburghii Tratt fermented nutritive liquid and its application |
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| WO2016127184A1 (en) * | 2015-02-06 | 2016-08-11 | University Of Florida Researchfoundation, Inc. | Bacteria and method for improving plant health and growth |
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| CN110419696A (en) * | 2019-06-28 | 2019-11-08 | 贵州大学 | A kind of Rosa roxburghii Tratt fermented nutritive liquid and its application |
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