CN115252520B

CN115252520B - Composite plant extract and application thereof

Info

Publication number: CN115252520B
Application number: CN202210931878.1A
Authority: CN
Inventors: 沈学三; 朱凯乐; 魏铮
Original assignee: Guangzhou Meichi Cosmetics Co ltd
Current assignee: Guangzhou Meichi Cosmetics Co ltd
Priority date: 2022-08-04
Filing date: 2022-08-04
Publication date: 2023-05-30
Anticipated expiration: 2042-08-04
Also published as: CN115252520A

Abstract

The invention discloses a compound plant extract and application thereof. The composite plant extract comprises the following components in percentage by mass: ginseng extract: 26% -68%; wolfberry extract: 10% -20%; tremella extract: 10% -20%; radix Ophiopogonis extract: 5% -15%; sugarcane root extract: 5% -15%; 1, 2-hexanediol: 1% -2%; 1, 2-pentanediol: 1% -2%; the ginseng extract, the medlar extract, the tremella extract, the dwarf lilyturf tuber extract and the sugarcane root extract are prepared by solid state fermentation, enzymolysis and ultrafiltration. The composite plant extract has the effects of lasting moisture retention, oxidation resistance, saccharification resistance, skin elasticity improvement and the like, has simple preparation process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Description

Composite plant extract and application thereof

Technical Field

The invention relates to the technical field of skin care, in particular to a composite plant extract and application thereof.

Background

The plant extract is a substance obtained by extracting or processing a plant (whole or a part of a plant) as a raw material with a suitable solvent or method, and can be used in various fields such as medicine, food, daily chemicals, and the like. At present, one or more plant extracts are added to many cosmetics, but the following problems are common: 1) Although the plant extract with a single component can exert certain effect, the action mechanism of the plant extract is single, the function is single, and the effect which can be exerted by the plant extract is greatly reduced due to the influence of a formula system or certain components in the formula; 2) Plant extracts added in cosmetic formulations are generally difficult to effectively absorb by the skin and have very limited practical effects.

Therefore, the development of the compound plant extract with various effects of lasting moisture preservation, oxidation resistance, saccharification resistance, skin elasticity improvement and the like has very important significance.

Disclosure of Invention

One of the objects of the present invention is to provide a complex plant extract.

The second object of the present invention is to provide a moisturizing composition to which the composite plant extract of the present invention is added.

The technical scheme adopted by the invention is as follows:

a composite plant extract, which comprises the following components in percentage by mass:

ginseng extract: 26% -68%;

wolfberry extract: 10% -20%;

tremella extract: 10% -20%;

radix Ophiopogonis extract: 5% -15%;

sugarcane root extract: 5% -15%;

1, 2-hexanediol: 1% -2%;

1, 2-pentanediol: 1% -2%;

the ginseng extract, the medlar extract, the tremella extract, the dwarf lilyturf tuber extract and the sugarcane root extract are prepared by solid state fermentation, enzymolysis and ultrafiltration.

Preferably, the ginseng extract, the medlar extract, the tremella extract, the dwarf lilyturf tuber extract and the sugarcane root extract are prepared by the following methods:

1) Cleaning and drying plant raw materials, and performing superfine grinding and screening to obtain plant raw material powder;

2) Dispersing plant raw material powder with water, and then performing high-pressure sterilization to obtain a solid plant culture medium;

3) Uniformly mixing the activated bacterial liquid and the sterilized sodium alginate solution, then dripping the mixture into the sterilized calcium chloride solution to form gel beads, and then aging to obtain embedded strain particles;

4) Mixing the embedded strain particles with a solid plant culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain a plant fermentation liquid;

5) Carrying out enzymolysis on the plant fermentation liquor to obtain an enzymatic hydrolysis plant fermentation liquor;

6) And (3) inactivating enzyme of the enzymatic plant fermentation broth, and performing ultrafiltration to obtain the plant extract.

Preferably, the ultrafiltration membrane used for ultrafiltration has a molecular weight cut-off of 1000Da to 2000Da.

Preferably, the ginseng extract is prepared by the following method:

1) Cleaning and drying ginseng, and then carrying out superfine grinding and screening to obtain ginseng powder;

2) Dispersing Ginseng radix powder with water, and sterilizing under high pressure to obtain solid Ginseng radix culture medium;

3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution, dripping into sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles with a solid ginseng culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain ginseng fermentation liquor;

5) Adjusting the pH value of the ginseng fermentation liquor to 1.0-2.0, adding pepsin for primary enzymolysis, adjusting the pH value to 6.0-7.0, and adding papain for secondary enzymolysis to obtain the ginseng fermentation liquor for enzymolysis;

6) Inactivating enzyme of the fermented Ginseng radix liquid, and ultrafiltering to obtain Ginseng radix extract.

Preferably, the drying in step 1) is carried out at a temperature of 50-55 ℃.

Preferably, the screening in the step 1) adopts a 60-80 mesh screen.

Preferably, the adding amount ratio of the ginseng powder to the water in the step 2) is 1g:1.3 mL-1 g:2.5mL.

Preferably, the temperature of the high-pressure sterilization in the step 2) is 121 ℃, and the sterilization time is 20-30 min.

Preferably, the aging time in the step 3) is 10 min-15 min.

Preferably, the fermentation in the step 4) is carried out at 30-35 ℃ for 3-7 days.

Preferably, the specific operation of adding water for dispersion in the step 4) is as follows: adding the fermented product into water, wherein the mass ratio of the fermented product to the water is 1:8.5-9.5, mashing, and then placing in a constant-temperature water bath for ultrasonic vibration at 20-30 ℃ for 1.5-2 h.

Preferably, the centrifugation in the step 4) is performed under the condition that the rotation speed of the centrifugal machine is 4000 r/min-5000 r/min, and the centrifugation time is 15 min-25 min.

Preferably, the first enzymolysis in the step 5) is carried out at the temperature of 35-40 ℃ for 2.5-3 hours.

Preferably, the second enzymolysis in the step 5) is carried out at 50-60 ℃ for 2.5-3 hours.

Preferably, the enzyme deactivation in the step 6) is carried out at 100-105 ℃ for 5-10 min.

Preferably, the ultrafiltration in step 6) is performed under a pressure of 1.5MPa to 2.5 MPa.

Preferably, the wolfberry extract is prepared by the following method:

1) Cleaning and drying the medlar, and then carrying out superfine grinding and screening to obtain medlar powder;

2) Dispersing the medlar powder with water, and then performing high-pressure sterilization to obtain a solid medlar culture medium;

3) Uniformly mixing the activated saccharomycete liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles with a solid medlar culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain medlar fermentation liquor;

5) Adjusting the pH value of the medlar fermentation broth to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymatic medlar fermentation broth;

6) And (3) inactivating enzyme of the enzymatic matrimony vine fermentation liquid, and performing ultrafiltration to obtain matrimony vine extract.

Preferably, the drying in step 1) is carried out at a temperature of 50-55 ℃.

Preferably, the screening in the step 1) adopts a 60-80 mesh screen.

Preferably, the adding amount ratio of the medlar powder to the water in the step 2) is 1g:1.3 mL-1 g:2.5mL.

Preferably, the aging time in the step 3) is 10 min-15 min.

Preferably, the enzymolysis in the step 5) is carried out at 40-50 ℃ for 2.5-3 hours.

Preferably, the tremella extract is prepared by the following method:

1) Cleaning and drying tremella, and then carrying out superfine grinding and screening to obtain tremella powder;

2) Dispersing tremella powder with water, and then performing high-pressure sterilization to obtain a solid tremella culture medium;

3) Uniformly mixing the activated saccharomycete liquid, the activated lactobacillus liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles with a solid tremella culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain tremella fermentation liquor;

5) Adjusting the pH value of the tremella fermentation liquor to 6.0-7.0, and then adding cellulase, pectase and neutral protease for enzymolysis to obtain the tremella fermentation liquor for enzymolysis;

6) And (3) inactivating enzyme of the enzymatic tremella fermentation liquid, and performing ultrafiltration to obtain tremella extract.

Preferably, the drying in step 1) is carried out at a temperature of 50-55 ℃.

Preferably, the screening in the step 1) adopts a 60-80 mesh screen.

Preferably, the adding amount ratio of the tremella powder to the water in the step 2) is 1g:1.3 mL-1 g:2.5mL.

Preferably, the aging time in the step 3) is 10 min-15 min.

Preferably, the ophiopogon root extract is prepared by the following method:

1) Cleaning and drying radix Ophiopogonis, and micronizing and sieving to obtain radix Ophiopogonis powder;

2) Dispersing radix Ophiopogonis powder with water, and performing high-pressure sterilization to obtain solid radix Ophiopogonis culture medium;

4) Mixing the embedded strain particles with a solid ophiopogon culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain ophiopogon fermentation liquor;

5) Adjusting the pH value of the ophiopogon japonicus fermentation liquor to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymolysis ophiopogon japonicus fermentation liquor;

6) And (3) inactivating enzyme of the enzymatic radix ophiopogonis fermentation liquid, and performing ultrafiltration to obtain the radix ophiopogonis extract.

Preferably, the drying in step 1) is carried out at a temperature of 50-55 ℃.

Preferably, the screening in the step 1) adopts a 60-80 mesh screen.

Preferably, the adding amount ratio of the dwarf lilyturf tuber powder to the water in the step 2) is 1g:1.3 mL-1 g:2.5mL.

Preferably, the aging time in the step 3) is 10 min-15 min.

Preferably, the sugarcane root extract is prepared by the following method:

1) Cleaning and drying the sugarcane roots, and carrying out superfine grinding and screening to obtain sugarcane root powder;

2) Dispersing the sugarcane root powder with water, and then performing high-pressure sterilization to obtain a solid sugarcane root culture medium;

4) Mixing the embedded strain particles with a solid-state sugarcane root culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain sugarcane root fermentation liquor;

5) Adjusting the pH value of the sugarcane root fermentation liquor to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymatic hydrolysis sugarcane root fermentation liquor;

6) And (3) inactivating enzyme of the enzymatic hydrolysis sugarcane root fermentation liquor, and performing ultrafiltration to obtain the sugarcane root extract.

Preferably, the drying in step 1) is carried out at a temperature of 50-55 ℃.

Preferably, the screening in the step 1) adopts a 60-80 mesh screen.

Preferably, the adding amount ratio of the sugarcane root powder to the water in the step 2) is 1g:1.3 mL-1 g:2.5mL.

Preferably, the aging time in the step 3) is 10 min-15 min.

Use of the above compound plant extract in cosmetics.

The essence comprises the following components in percentage by mass:

humectant: 7% -12%;

chelating agent: 0.01 to 0.1 percent;

and (3) a thickening agent: 0.1 to 0.2 percent;

the above compound plant extract: 0.5% -20%;

preservative: 0.2% -1%;

water: 70% -90%.

Preferably, the humectant is at least one of glycerin, butylene glycol and betaine.

Preferably, the chelating agent is at least one of disodium EDTA and calcium citrate.

Preferably, the thickener is at least one of carrageenan and hydrolyzed polysaccharide gum.

Preferably, the preservative is at least 2 of p-hydroxyacetophenone, 1, 2-hexanediol, methylparaben and phenoxyethanol.

The essence cream comprises the following components in percentage by mass:

phase A:

humectant: 5% -10%; disodium EDTA: 0.01 to 0.1 percent; water: 60% -80%;

and B phase:

emulsifying agent: 1% -3%; oil ester: 5% -10%;

and C phase:

and (3) a thickening agent: 1% -2%;

and D phase:

the above compound plant extract: 0.5% -20%; preservative: 0.5 to 1.5 percent.

Preferably, the emulsifier is emulsifier Montanov 82 (main ingredient: cetyl stearyl alcohol and cocoyl glucoside), emulsifier Montanov 202 (main ingredient: arachidyl alcohol, behenyl alcohol and arachidyl glucoside), and emulsifier SENSANOV WR (main ingredient: C) ₂₀ ～C ₂₂ Alcohol phosphate and C ₂₀ ～C ₂₂ Alcohol).

Preferably, the grease is at least one of caprylic/capric triglyceride, polydimethylsiloxane, isononyl isononanoate and mineral oil.

Preferably, the thickener is at least one of a thickener SEPINOV EMT 10 (main component: hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer), and a thickener SEPIPLUS 400 (main component: polyacrylate-13, polyisobutylene and polysorbate-20).

The beneficial effects of the invention are as follows: the composite plant extract has the effects of lasting moisture retention, oxidation resistance, saccharification resistance, skin elasticity improvement and the like, has simple preparation process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Specifically:

1) The compound plant extract is prepared from ginseng, medlar, tremella, dwarf lilyturf tuber and sugarcane roots through solid state fermentation, enzymolysis and ultrafiltration, so that the extraction rate of active ingredients is maximized, plant extracts containing a plurality of active ingredients such as polysaccharide, saponin, vitamin, amino acid, polypeptide and the like are obtained, the molecular weight of each active ingredient in the plant extracts is controlled below 2000Da through ultrafiltration, the efficient absorption of skin is facilitated, and in addition, the synergistic effect among the plurality of plant extracts can be fully exerted by compounding, and the action effect of the compound plant extract can be highlighted;

2) The composite plant extract has simple production process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Detailed Description

The invention is further illustrated and described below in connection with specific examples.

Example 1:

a composite plant extract having the composition shown in the following table:

TABLE 1 composition of composite plant extracts

Raw materials	Mass percent (%)
		Ginseng extract	47
Lycium barbarum extract	15
		Tremella extract	15
Radix Ophiopogonis extract	10
		Sugarcane root extract	10
1, 2-hexanediol	1.5
		1, 2-pentanediol	1.5

Note that:

the ginseng extract is prepared by the following steps: 1) Cleaning Ginseng radix, oven drying at 55deg.C, micronizing with an ultrafine pulverizer, and sieving with 80 mesh sieve to obtain Ginseng radix powder; 2) Stirring and dispersing 10g of ginseng powder with 25mL of deionized water, and then filling into an autoclave, and performing high-pressure sterilization at 121 ℃ for 30min to obtain a solid ginseng culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, dripping the mixture into sterilized calcium chloride solution with the mass fraction of 4% by using a syringe to form gel beads, standing for 10min for aging, filtering, and flushing the filtered solid with sterile water to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid ginseng culture medium, putting the mixture into a constant temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermented product into deionized water, wherein the mass ratio of the fermented product to the deionized water is 1:9, mashing, putting the crushed mixture into a constant temperature water bath, ultrasonically vibrating the crushed mixture at 25 ℃ for 2 hours, centrifuging the mixture for 25 minutes at the rotation speed of a centrifuge of 5000r/min, and taking supernatant to obtain ginseng fermentation liquor; 5) Adjusting the pH value of the ginseng fermentation liquor to 1.5, adding pepsin, carrying out enzymolysis for 3 hours at 37 ℃, adjusting the pH value to 6.5, adding papain, carrying out enzymolysis for 3 hours at 50 ℃ to obtain an enzymolysis ginseng fermentation liquor; 6) Heating the enzymolysis Ginseng radix fermentation broth to 105deg.C, inactivating enzyme for 10min, cooling to 25deg.C, and ultrafiltering with ultrafiltration membrane with molecular weight cut-off of 2000Da under pressure of 2.5MPa to obtain Ginseng radix extract.

The medlar extract is prepared by the following steps: 1) Cleaning fructus Lycii, oven drying at 55deg.C, micronizing with an ultrafine pulverizer, and sieving with 80 mesh sieve to obtain fructus Lycii powder; 2) Stirring and dispersing 10g of medlar powder with 25mL of deionized water, and then filling the mixture into an autoclave, and carrying out high-pressure sterilization for 30min at 121 ℃ to obtain a solid medlar culture medium; 3) Uniformly mixing the activated saccharomycete liquid and a sterilized sodium alginate solution with the mass fraction of 3 percent according to the volume ratio of 1:5, dripping the mixture into a sterilized calcium chloride solution with the mass fraction of 4 percent by using a syringe to form gel beads, standing for 10 minutes for aging, filtering, and flushing the filtered solid by using sterile water to obtain embedded strain particles; 4) Mixing the embedded strain particles and a solid medlar culture medium, putting the mixture into a constant temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermented product into deionized water, wherein the mass ratio of the fermented product to the deionized water is 1:9, mashing, putting the mixture into a constant temperature water bath, ultrasonically vibrating for 2 hours at 25 ℃, centrifuging for 25 minutes at the rotation speed of a centrifuge of 5000r/min, and taking supernatant to obtain medlar fermentation liquor; 5) Adjusting the pH value of the medlar fermentation broth to 6.5, adding cellulase and pectase, and performing enzymolysis for 3 hours at 50 ℃ to obtain the enzymatic medlar fermentation broth; 6) And (3) heating the enzymatic hydrolysis fermentation liquid of the Chinese wolfberry, inactivating enzyme for 10min after the temperature is raised to 105 ℃, cooling to 25 ℃, and performing ultrafiltration by using an ultrafiltration membrane with the molecular weight cutoff of 2000Da under the condition of the pressure of 2.5MPa to obtain the Chinese wolfberry extract.

The tremella extract is prepared by the following steps: 1) Cleaning Tremella, oven drying at 55deg.C, micronizing with an ultrafine pulverizer, and sieving with 60 mesh sieve to obtain Tremella powder; 2) Stirring and dispersing 10g of tremella powder with 25mL of deionized water, and then filling the tremella powder into an autoclave, and carrying out high-pressure sterilization for 30min at 121 ℃ to obtain a solid tremella culture medium; 3) Uniformly mixing the activated saccharomycete liquid, the activated lactobacillus liquid and the sterilized sodium alginate solution with the mass fraction of 3 percent according to the volume ratio of 1:1:8, dripping the mixture into the sterilized calcium chloride solution with the mass fraction of 4 percent by using a syringe to form gel beads, standing for 10 minutes for aging, filtering, and flushing the filtered solid by using sterile water to obtain embedded strain particles; 4) Mixing the embedded strain particles and a solid tremella culture medium, putting the mixture into a constant temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermented product into deionized water, wherein the mass ratio of the fermented product to the deionized water is 1:9, mashing, putting the mixture into a constant temperature water bath, ultrasonically vibrating for 2 hours at 25 ℃, centrifuging for 25 minutes at the rotation speed of a centrifuge of 5000r/min, and taking supernatant to obtain tremella fermentation liquor; 5) Adjusting the pH value of the tremella fermentation liquor to 6.5, adding cellulase, pectase and neutral protease, and carrying out enzymolysis for 3 hours at 50 ℃ to obtain the tremella fermentation liquor subjected to enzymolysis; 6) And (3) heating the enzymatic tremella fermentation liquid to 105 ℃, inactivating enzymes for 10min, cooling to 25 ℃, and performing ultrafiltration with an ultrafiltration membrane with a molecular weight cutoff of 2000Da under the condition of 2.5MPa to obtain tremella extract.

The radix ophiopogonis extract is prepared by the following steps: 1) Cleaning radix Ophiopogonis, oven drying at 55deg.C, micronizing with an ultrafine pulverizer, and sieving with 60 mesh sieve to obtain radix Ophiopogonis powder; 2) Stirring and dispersing 10g of radix Ophiopogonis powder with 25mL of deionized water, and then placing into an autoclave, and performing high-pressure sterilization at 121 ℃ for 30min to obtain a solid radix Ophiopogonis culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, dripping the mixture into sterilized calcium chloride solution with the mass fraction of 4% by using a syringe to form gel beads, standing for 10min for aging, filtering, and flushing the filtered solid with sterile water to obtain embedded strain particles; 4) Mixing the embedded strain particles and a solid ophiopogon culture medium, putting the mixture into a constant temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermented product into deionized water, wherein the mass ratio of the fermented product to the deionized water is 1:9, mashing, putting the crushed mixture into a constant temperature water bath, ultrasonically vibrating for 2 hours at 25 ℃, centrifuging for 25 minutes at the rotation speed of a centrifuge of 5000r/min, and taking supernatant to obtain ophiopogon fermentation liquor; 5) Adjusting the pH value of the ophiopogon japonicus fermentation liquor to 6.5, adding cellulase and pectase, and carrying out enzymolysis for 3 hours at 50 ℃ to obtain the enzymolysis ophiopogon japonicus fermentation liquor; 6) Heating the enzymolysis radix Ophiopogonis fermentation broth to 105deg.C, inactivating enzyme for 10min, cooling to 25deg.C, and ultrafiltering with ultrafiltration membrane with molecular weight cut-off of 2000Da under pressure of 2.5MPa to obtain radix Ophiopogonis extract.

The sugarcane root extract is prepared by the following steps: 1) Cleaning the sugarcane roots, drying at 55 ℃, carrying out superfine grinding by using a superfine grinder, and then sieving by using a 60-mesh screen to obtain sugarcane root powder; 2) Stirring and dispersing 10g of sugarcane root powder with 25mL of deionized water, and then filling the mixture into an autoclave, and carrying out high-pressure sterilization for 30min at 121 ℃ to obtain a solid sugarcane root culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, dripping the mixture into sterilized calcium chloride solution with the mass fraction of 4% by using a syringe to form gel beads, standing for 10min for aging, filtering, and flushing the filtered solid with sterile water to obtain embedded strain particles; 4) Mixing the embedded strain particles and a solid sugarcane root culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermented product into deionized water, wherein the mass ratio of the fermented product to the deionized water is 1:9, mashing, putting the crushed mixture into a constant-temperature water bath, ultrasonically vibrating for 2 hours at 25 ℃, centrifuging for 25 minutes at the rotation speed of a centrifuge of 5000r/min, and taking supernatant to obtain sugarcane root fermentation liquor; 5) Adjusting the pH value of the sugarcane root fermentation liquor to 6.5, adding cellulase and pectase, and carrying out enzymolysis for 3 hours at 50 ℃ to obtain the enzymolysis sugarcane root fermentation liquor; 6) Heating the enzymatic hydrolysis fermentation liquor of the sugarcane roots to 105 ℃, inactivating enzymes for 10min, cooling to 25 ℃, and performing ultrafiltration by using an ultrafiltration membrane with a molecular weight cut-off of 2000Da under the condition of 2.5MPa to obtain the sugarcane root extract.

Example 2:

a composite plant extract having the composition shown in the following table:

TABLE 2 composition of composite plant extracts

Raw materials	Mass percent (%)
		Ginseng extract	49
Lycium barbarum extract	13
		Tremella extract	15
Radix Ophiopogonis extract	5
		Sugarcane root extract	15
1, 2-hexanediol	2
		1, 2-pentanediol	1

Note that: the preparation methods of ginseng extract, wolfberry extract, tremella extract, ophiopogon root extract and sugarcane root extract are the same as in example 1.

Example 3:

a composite plant extract having the composition shown in the following table:

TABLE 3 composition of composite plant extracts

Raw materials	Mass percent (%)
		Ginseng extract	37
Lycium barbarum extract	20
		Tremella extract	20
Radix Ophiopogonis extract	10
		Sugarcane root extract	10
1, 2-hexanediol	1.5
		1, 2-pentanediol	1.5

Example 4:

a composite plant extract having the composition shown in the following table:

TABLE 4 composition of composite plant extracts

Raw materials	Mass percent (%)
		Ginseng extract	27
Lycium barbarum extract	20
		Tremella extract	20
Radix Ophiopogonis extract	15
		Sugarcane root extract	15
1, 2-hexanediol	1
		1, 2-pentanediol	2

Comparative example 1:

a composite plant extract having the composition shown in the following table:

TABLE 5 composition of composite plant extracts

Note that:

the ginseng extract is prepared by the following steps: 1) Cleaning Ginseng radix, oven drying at 55deg.C, micronizing with an ultrafine pulverizer, and sieving with 80 mesh sieve to obtain Ginseng radix powder; 2) Dispersing 10g of ginseng powder with 90mL of deionized water under stirring, soaking for 1h, decocting at 80 ℃ for 1h, filtering, adding filter residues into 90mL of deionized water, decocting at 80 ℃ for 1h, filtering, combining the two filtrates, concentrating to 10mL, centrifuging at a centrifuge rotating speed of 5000r/min for 25min, filtering and sterilizing the supernatant to obtain the ginseng extract.

The preparation method of the medlar extract, the tremella extract, the dwarf lilyturf tuber extract and the sugarcane root extract is the same as that of the ginseng extract.

Comparative example 2:

ginseng radix extract (preparation method is the same as in example 1).

Application example 1:

an essence, the composition of which is shown in the following table:

TABLE 6 composition of essence

Raw materials	Mass percent (%)
		Glycerol	6.0
Butanediol (butanediol)	4.0
		EDTA disodium salt	0.02
Hydrolyzed polysaccharide gum	0.1
		The Complex plant extract of example 1	3.0
Para hydroxy acetophenone	0.5
		1, 2-hexanediol	0.5
Deionized water	85.88

The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, EDTA disodium and hydrolyzed polysaccharide gum in deionized water, heating to 80deg.C, maintaining the temperature for 20min, cooling to 45deg.C, adding the compound plant extract of example 1 and p-hydroxyacetophenone and 1, 2-hexanediol, and mixing to obtain essence.

Application example 2:

an essence, the composition of which is shown in the following table:

TABLE 7 composition of essence

The preparation method of the essence comprises the following steps: dissolving glycerol, betaine, EDTA disodium and carrageenan in deionized water, heating to 80deg.C, maintaining the temperature for 20min, cooling to 45deg.C, adding the compound plant extract of example 1, preheating and mixing to transparent 1, 2-hexanediol, methylparaben and phenoxyethanol, and mixing to obtain essence.

Application example 3:

an essence cream, the composition of which is shown in the following table:

table 8 composition table of essence cream

The preparation method of the essence cream comprises the following steps: mixing and stirring the phase A raw material and the phase B raw material respectively, heating to 85 ℃, adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C, homogenizing for 6min again, preserving heat for 20min, cooling to 45 ℃, adding the composite plant extract of the embodiment 1 in the phase D, preheating and dissolving to transparent p-hydroxyacetophenone and 1, 2-hexanediol, and uniformly mixing to obtain the essence cream.

Application example 4:

an essence cream, the composition of which is shown in the following table:

table 9 composition table of essence cream

The preparation method of the essence cream comprises the following steps: mixing and stirring the phase A raw material and the phase B raw material respectively, heating to 85 ℃, adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C, homogenizing for 6min again, preserving heat for 20min, cooling to 45 ℃, adding the composite plant extract of the embodiment 1 in the phase D, and preheating to dissolve to transparent 1, 2-hexanediol, methylparaben and phenoxyethanol, and mixing uniformly to obtain the essence cream.

Comparative application example 1:

an essence, the composition of which is shown in the following table:

table 10 composition table of essence

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The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, EDTA disodium and hydrolyzed polysaccharide gum in deionized water, heating to 80deg.C, maintaining the temperature for 20min, cooling to 45deg.C, adding the compound plant extract of comparative example 1, preheating and mixing to transparent p-hydroxyacetophenone and 1, 2-hexanediol, and mixing to obtain essence.

Comparative application example 2:

an essence, the composition of which is shown in the following table:

table 11 composition table of essence

Raw materials	Mass percent (%)
		Glycerol	6.0
Butanediol (butanediol)	4.0
		EDTA disodium salt	0.02
Hydrolysis of polysaccharide acids	0.1
		Ginseng radix extract of comparative example 2	3.0
Para hydroxy acetophenone	0.5
		1, 2-hexanediol	0.5
Deionized water	85.88

The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, EDTA disodium and hydrolyzed polysaccharide gum in deionized water, heating to 80deg.C, maintaining the temperature for 20min, cooling to 45deg.C, adding Ginseng radix extract of comparative example 2, preheating and mixing to transparent p-hydroxyacetophenone and 1, 2-hexanediol, and mixing to obtain essence.

Comparative application example 3:

an essence cream, the composition of which is shown in the following table:

table 12 composition table of essence cream

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The preparation method of the essence cream comprises the following steps: and respectively mixing and stirring the phase A raw material and the phase B raw material uniformly, heating to 85 ℃, adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C, homogenizing for 6min again, preserving heat for 20min, cooling to 45 ℃, adding the composite plant extract of the comparative example 1 in the phase D, and preheating to dissolve to transparent 1, 2-hexanediol, methylparaben and phenoxyethanol, and uniformly mixing to obtain the essence cream.

Comparative application example 4:

an essence cream, the composition of which is shown in the following table:

TABLE 13 composition of essence cream

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The preparation method of the essence cream comprises the following steps: mixing and stirring the phase A raw material and the phase B raw material respectively, heating to 85 ℃, adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C, homogenizing for 6min again, preserving heat for 20min, cooling to 45 ℃, adding the ginseng extract of comparative example 2 in the phase D, and preheating to dissolve to transparent 1, 2-hexanediol, methylparaben and phenoxyethanol, and mixing uniformly to obtain the essence cream.

Comparative application example 5:

european Bedses moisturizing repair stock (Guangzhou, makeup Co., ltd.).

Comparative application example 6:

european Bei Sishui moisturizing cream (Guangzhou City, makeup Co., ltd.).

Performance test:

1) Determination of skin moisture content:

test standard: QB/T4256-2011, cosmetic moisturizing efficacy evaluation guide.

Test object: 200 volunteers aged 18 to 60 years (without limitation for men and women, except for gestational and lactating women), had healthy skin, no history of skin allergy, and no other clinical trial was taken in the test sites for nearly three months, and 200 volunteers were randomly divided into 8 groups (designated as group A, group B, group C, group D, group E, group F, group G and group H) of 25 persons each.

Test environment: the temperature is 20-22 ℃ and the relative humidity is 40-60%, and the real-time dynamic detection is carried out.

The testing method comprises the following steps:

a) Any product cannot be used 2-3 days before the test of the tested part, water cannot be contacted for 1-3 hours, a mark of 3cm multiplied by 3cm is made on the inner side of the forearm, the tested part is wiped by dry facial tissue before the test, and then the tested part is stood still for 20min;

b) H 0 (blank test): the test areas were measured in parallel 3 times using a MoistureMeter SC stratum corneum moisture content measuring instrument, with the test samples at 2.0mg/cm ² ±0.1mg/cm ² Single coating is carried out on the dosage of the (B) and the actual coating sample quantity is recorded;

c) 1h (experimental test): measuring the test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and carrying out parallel measurement for 3 times; h 2 (experimental test): measuring the test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and carrying out parallel measurement for 3 times; h 4 (experimental test): measuring the test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and carrying out parallel measurement for 3 times;

d) The essence of application example 1 is used in group a, the essence of application example 4 is used in group B, the essence of comparative application example 1 is used in group C, the essence of comparative application example 3 is used in group D, the essence of comparative application example 2 is used in group E, the essence of comparative application example 4 is used in group F, the European style moisturizing and repairing stock of comparative application example 5 is used in group G, and the European style Bei Sishui moisturizing and repairing cream of comparative application example 6 is used in group H.

The test indexes are as follows:

a) The higher the moisture content of the stratum corneum, the higher the value, indicating a higher moisture content of the stratum corneum;

b) Percent change (%) = (n hours number-0 hours number)/0 hours number x 100%;

c) Total effective rate (%) =effective number/total number×100%.

Calculating a test result:

a) Descriptive statistics are carried out on the measured values of all the test areas, wherein the descriptive statistics comprise average values, standard deviations, minimum values, median values and maximum values;

b) Using SPSS 22.0 analysis, the differences before and after the test are compared by applying paired sample T test to normal distribution, and the differences are compared by applying rank and test to normal distribution, wherein P is less than or equal to 0.05, the differences are significant, if P is more than or equal to 0.05, the differences are not significant, the statistical method adopts double-tail detection, and the detection level alpha=0.05.

The test results are shown in tables 14 to 29:

table 14A comparison of results of water content in stratum corneum of subject and control subjects (unit: a.u.)

Table 15 results of percentage change in stratum corneum Water content of group A subjects

Cycle time	1h	2h	4h
				Percent Change (%)	141.18	135.40	139.06

Table 16B comparison of results of water content in stratum corneum of subject and control (unit: a.u.)

Table 17 results of percentage change in stratum corneum Water content of group B subjects

Cycle time	1h	2h	4h
				Percent Change (%)	139.20	133.74	136.09

Table 18C comparison of results of water content in stratum corneum of subject and control of subjects (unit: a.u.)

Table 19 results of percentage change in stratum corneum Water content of group C subjects

Cycle time	1h	2h	4h
				Percent Change (%)	76.78	69.49	47.89

Table 20D comparison of results of water content in stratum corneum of subject and control subjects (unit: a.u.)

Table 21 percent change results of stratum corneum Water content in group D subjects

Cycle time	1h	2h	4h
				Percent Change (%)	76.30	61.41	49.75

Table 22E comparison of results of water content in stratum corneum of subject and blank control (unit: a.u.)

Table 23 percent change results of stratum corneum Water content in group E subjects

Cycle time	1h	2h	4h
				Percent Change (%)	78.03	70.69	48.94

Table 24F comparison of results of water content in stratum corneum of subject and control subjects (unit: a.u.)

Table 25 percent change results of stratum corneum Water content in group F subjects

Cycle time	1h	2h	4h
				Percent Change (%)	71.98	60.27	47.05

Table 26G comparison of results of water content in stratum corneum of subject and control of subjects (unit: a.u.)

Table 27 percent change results of stratum corneum Water content in group G subjects

Cycle time	1h	2h	4h
				Percent Change (%)	85.45	99.69	40.18

Table 28H comparison of results of water content in stratum corneum of subject and control subjects (unit: a.u.)

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Table 29 percent change results of stratum corneum Water content in group H subjects

Cycle time	1h	2h	4h
				Percent Change (%)	87.69	99.65	45.47

Tables 14 to 29 show that: the difference before and after the test is subjected to normal distribution by using SPSS 22.0 analysis, and the difference between the blank control area difference value of the group A and the blank control area difference value of the group B and the difference between the tested area difference value of the group A and the blank control area difference value of the tested area difference value of the group B are less than or equal to 0.05 when the difference between the blank control area difference value and the tested area difference value of the group A and the blank control area difference value of the tested area difference value of the group B and the tested area difference value of the tested area difference between the blank control area difference value and the tested area difference value of the tested area difference between the blank control area and the tested area difference between the blank control area and the tested area difference between the tested area and the tested area are less than or equal to 0.05 when the difference between the blank control area and the blank control area difference between the blank area and the tested area are 1h and; the difference between the blank control areas of the group C, the group D, the group E and the group F and the difference between the blank control areas of the group F and the tested areas are less than or equal to 0.05 at 1H, and P is more than 0.05 at 2H and 4H, which indicates that the water content of the stratum corneum is remarkably different before 1H compared with that of the tested samples without any product, the tested samples have moisturizing effect, no remarkable difference exists after 2H, the tested samples have no moisturizing effect, P is less than or equal to 0.05 at 1H and 2H of the group G and the group H, and P is more than 0.05 at 4H, which indicates that the tested samples have moisturizing effect before 2H and have no moisturizing effect after 4H.

In summary, the essence of application example 1 and the essence cream of application example 4 have more remarkable moisturizing effect in the test, and the duration of the moisturizing effect is longer, which indicates that the composite plant extract of the invention has more remarkable moisturizing effect and longer moisturizing time in the cosmetic than the composite plant extract and the single ginseng extract prepared by the common extraction method.

2) Skin elasticity test:

test object: 80 volunteers aged 18-60 years (without limitation for men and women, except for gestational and lactating women), healthy skin, no history of skin allergy, no other clinical trial at the test sites for nearly three months, and the 80 volunteers were randomly divided into 8 groups (designated as group a, group B, group C, group D, group E, group F, group G and group H) of 10 persons each.

The testing method comprises the following steps: the essence of application example 1 was used in group a, the essence of application example 4 was used in group B, the essence of comparative application example 1 was used in group C, the essence of comparative application example 3 was used in group D, the essence of comparative application example 2 was used in group E, the essence of comparative application example 4 was used in group F, the European style moisturizing and repairing stock of comparative application example 5 was used in group G, the European style Bei Sishui moisturizing and repairing cream of comparative application example 6 was used in group H, and the skin elasticity tester revismeter RV600 was used for measuring the measured parts at the same time before, after 1 week, after 2 weeks and after 4 weeks of use, respectively, continuously for 4 weeks.

The test results are shown in table 30:

table 30 skin elasticity test results

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Note that: percent change in elasticity (%) = (n weeks for Rn-before Rn) x 100% before Rn.

As can be seen from table 30: the volunteers used the essence of application example 1 and the essence cream of application example 4, which showed the most remarkable increase in skin elasticity index after 4 weeks, and showed that the skin elasticity was greatly improved, indicating that the composite plant extract of the present invention was easier to penetrate and absorb in cosmetics, and increased skin elasticity and water content of horny layer than the composite plant extract and single ginseng extract prepared by the general extraction method.

3) Oxidative stress test:

the inspection principle is as follows: oxidative stress is one of the important causes of aging and injury of skin cells, and the excessive production of free radicals by oxygen metabolism of the body can lead to skin aging and induce the formation of skin wrinkles. Vitamin C (ascorbic acid) is an effective free radical scavenger that is converted to dehydroascorbic acid by stepwise electron supply to scavenge DPPH and like free radicals.

The testing method comprises the following steps:

a) Test samples were divided into 8 groups: the essence of application example 1 is used in group a, the essence of application example 4 is used in group B, the essence of comparative application example 1 is used in group C, the essence of comparative application example 3 is used in group D, the essence of comparative application example 2 is used in group E, the essence of comparative application example 4 is used in group F, the European style moisturizing and repairing stock of comparative application example 5 is used in group G, and the European style Bei Sishui moisturizing and repairing cream of comparative application example 6 is used in group H;

b) Accurately weighing a test sample and vitamin C, respectively adding a proper amount of absolute ethyl alcohol for dilution, shaking uniformly, preparing a series of concentrations of vitamin C solution by using absolute ethyl alcohol for standby, respectively mixing the test sample solution and the vitamin C solution with a series of concentrations with DPPH solution uniformly, standing at room temperature in a dark place for 30min, measuring absorbance at 515nm, taking vitamin C as a positive control, taking each group of samples for 2 times, and taking an average value.

The test results are shown in table 31:

TABLE 31 determination of DPPH radical scavenger of test samples

As can be seen from table 31: the essence of application example 1 and the essence of application example 4 have much higher scavenging rate on DPPH free radical than other control samples, i.e., application example 1 and application example 4 have the strongest antioxidant ability, indicating that the compound plant extract of the present invention has stronger antioxidant ability than the compound plant extract prepared by the general extraction method and the single ginseng extract.

4) Anti-glycation test:

the testing method comprises the following steps:

a) The test samples were divided into 8 groups, group a using the essence of application example 1, group B using the essence of application example 4, group C using the essence of comparative application example 1, group D using the essence of comparative application example 3, group E using the essence of comparative application example 2, group F using the essence of comparative application example 4, group G using the obex moisturizing and repairing stock solution of comparative application example 5, group H using the euro Bei Sishui moisturizing and repairing cream of comparative application example 6, and the 8 groups of samples were diluted into 10% aqueous solutions, respectively;

b) 1mL of sample and 1mL of fructose solution (1.5 mol/L) are added into a test tube under the aseptic condition, evenly mixed, incubated for 2 hours in a constant temperature incubator at 37 ℃, then BSA (bovine serum albumin) solution (30 mg/mL) and 1mL of BSA (bovine serum albumin) solution are added, all reactants are dissolved by using a phosphoric acid buffer solution containing 0.1% of sodium azide, an aminoguanidine with the same mass concentration is used as a positive representative, the phosphate buffer solution is used as a blank control, the blank control is placed into the incubator at a fixed temperature for reaction for 6 days at 37 ℃, the fluorescence intensity of the sample is measured under the conditions that the excitation wavelength is 370nm and the emission wavelength is 440nm, each group of measurement is carried out twice, Taking an average value, and calculating a calculation formula of the generation inhibition rate R of AGEs: r (%) = (1-F) _A /F _B ) X 100%, where F _A For the fluorescence intensity of each sample group, F _B Fluorescence intensity for the blank.

The test results are shown in table 32:

table 32 test sample for inhibition of fluorescent AGEs production

As can be seen from table 32: the highest inhibition ratio of the essence of application example 1 and the essence of application example 4 to fluorescent AGEs, namely the strongest anti-saccharification ability of application example 1 and application example 4, shows that the anti-saccharification ability of the composite plant extract of the invention is more prominent compared with the composite plant extract prepared by a common extraction method and a single ginseng extract.

5) Skin feel test:

test object: 80 volunteers aged 18 to 60 years (without limitation for men and women, except for women in gestation and lactation), skin health, no history of skin allergy, were randomly divided into 8 groups (designated as group a, group B, group C, group D, group E, group F, group H and group G) of 10 persons each.

The testing method comprises the following steps: the essence of application example 1 was used in group a, the essence of application example 4 was used in group B, the essence of comparative application example 1 was used in group C, the essence of comparative application example 3 was used in group D, the essence of comparative application example 2 was used in group E, the essence of comparative application example 4 was used in group F, the European style moisturizing and repairing stock solution of comparative application example 5 was used in group G, the European style Bei Sishui moisturizing and repairing cream of comparative application example 6 was used in group H, and the procedure was continued for 4 weeks, and each group was measured twice, taking the average value of each group.

The test results are shown in table 33:

TABLE 33 skin feel test results

Note that: the score of each item is 0-10 points, 0 points being worst, 10 points being best.

As can be seen from table 33: the essence of application example 1 and the essence of application example 4 have the highest scores, which show that the composite plant extract of the invention has good spreadability in cosmetics, is easy to absorb, is not sticky and is more easily accepted and favored by consumers compared with the composite plant extract prepared by a common extraction method and a single ginseng extract.

Furthermore, by performing the same test, it was found that: the effects of the composite plant extracts of examples 2 to 4 in terms of moisture retention, oxidation resistance, saccharification resistance, skin elasticity improvement, etc. were very similar to those of the composite plant extract of example 1.

The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.

Claims

1. The composite plant extract is characterized by comprising the following components in percentage by mass:

Ginseng extract: 26% -68%;

wolfberry extract: 10% -20%;

tremella extract: 10% -20%;

radix Ophiopogonis extract: 5% -15%;

sugarcane root extract: 5% -15%;

1, 2-hexanediol: 1% -2%;

1, 2-pentanediol: 1% -2%;

the ginseng extract, the medlar extract, the tremella extract, the dwarf lilyturf tuber extract and the sugarcane root extract are prepared by solid state fermentation, enzymolysis and ultrafiltration;

the ginseng extract is prepared by the following steps: 1) Cleaning and drying ginseng, and then carrying out superfine grinding and screening to obtain ginseng powder; 2) Dispersing Ginseng radix powder with water, and sterilizing under high pressure to obtain solid Ginseng radix culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution, dripping into sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid ginseng culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain ginseng fermentation liquor; 5) Adjusting the pH value of the ginseng fermentation liquor to 1.0-2.0, adding pepsin for primary enzymolysis, adjusting the pH value to 6.0-7.0, and adding papain for secondary enzymolysis to obtain the ginseng fermentation liquor for enzymolysis; 6) Inactivating enzyme of the enzymolysis Ginseng radix fermentation broth, and ultrafiltering to obtain Ginseng radix extract;

The medlar extract is prepared by the following steps: 1) Cleaning and drying the medlar, and then carrying out superfine grinding and screening to obtain medlar powder; 2) Dispersing the medlar powder with water, and then performing high-pressure sterilization to obtain a solid medlar culture medium; 3) Uniformly mixing the activated saccharomycete liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid medlar culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain medlar fermentation liquor; 5) Adjusting the pH value of the medlar fermentation broth to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymatic medlar fermentation broth; 6) Inactivating enzyme of the enzymatic matrimony vine fermentation liquid, and performing ultrafiltration to obtain matrimony vine extract;

the tremella extract is prepared by the following steps: 1) Cleaning and drying tremella, and then carrying out superfine grinding and screening to obtain tremella powder; 2) Dispersing tremella powder with water, and then performing high-pressure sterilization to obtain a solid tremella culture medium; 3) Uniformly mixing the activated saccharomycete liquid, the activated lactobacillus liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid tremella culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain tremella fermentation liquor; 5) Adjusting the pH value of the tremella fermentation liquor to 6.0-7.0, and then adding cellulase, pectase and neutral protease for enzymolysis to obtain the tremella fermentation liquor for enzymolysis; 6) Inactivating enzyme of the zymolysis tremella fermentation liquid, and performing ultrafiltration to obtain tremella extract;

The ophiopogon root extract is prepared by the following method: 1) Cleaning and drying radix Ophiopogonis, and micronizing and sieving to obtain radix Ophiopogonis powder; 2) Dispersing radix Ophiopogonis powder with water, and performing high-pressure sterilization to obtain solid radix Ophiopogonis culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution, dripping into sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid ophiopogon culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain ophiopogon fermentation liquor; 5) Adjusting the pH value of the ophiopogon japonicus fermentation liquor to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymolysis ophiopogon japonicus fermentation liquor; 6) Inactivating enzyme of the enzymatic radix Ophiopogonis fermentation broth, and ultrafiltering to obtain radix Ophiopogonis extract;

the sugarcane root extract is prepared by the following steps: 1) Cleaning and drying the sugarcane roots, and carrying out superfine grinding and screening to obtain sugarcane root powder; 2) Dispersing the sugarcane root powder with water, and then performing high-pressure sterilization to obtain a solid sugarcane root culture medium; 3) Uniformly mixing activated lactobacillus bacteria solution and sterilized sodium alginate solution, dripping into sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles; 4) Mixing the embedded strain particles with a solid-state sugarcane root culture medium, fermenting, adding water for dispersion, and centrifuging to obtain supernatant to obtain sugarcane root fermentation liquor; 5) Adjusting the pH value of the sugarcane root fermentation liquor to 6.0-7.0, and then adding cellulase and pectase for enzymolysis to obtain the enzymatic hydrolysis sugarcane root fermentation liquor; 6) And (3) inactivating enzyme of the enzymatic hydrolysis sugarcane root fermentation liquor, and performing ultrafiltration to obtain the sugarcane root extract.

2. The compound plant extract of claim 1, wherein: the molecular weight cut-off of the ultrafiltration membrane adopted by the ultrafiltration is 1000 Da-2000 Da.

3. Use of the complex plant extract of claim 1 or 2 in cosmetics.

4. The essence is characterized by comprising the following components in percentage by mass:

humectant: 7% -12%;

chelating agent: 0.01 to 0.1 percent;

and (3) a thickening agent: 0.1 to 0.2 percent;

the complex plant extract of claim 1 or 2: 0.5% -20%;

preservative: 0.2% -1%;

water: 70% -90%.

5. The essence cream is characterized by comprising the following components in percentage by mass:

phase A:

humectant: 5% -10%; disodium EDTA: 0.01 to 0.1 percent; water: 60% -80%;

and B phase:

emulsifying agent: 1% -3%; oil ester: 5% -10%;

and C phase:

and (3) a thickening agent: 1% -2%;

and D phase:

the complex plant extract of claim 1 or 2: 0.5% -20%; preservative: 0.5 to 1.5 percent.