CN115252520A

CN115252520A - Composite plant extract and application thereof

Info

Publication number: CN115252520A
Application number: CN202210931878.1A
Authority: CN
Inventors: 沈学三; 朱凯乐; 魏铮
Original assignee: Guangzhou Meichi Cosmetics Co ltd
Current assignee: Guangzhou Meichi Cosmetics Co ltd
Priority date: 2022-08-04
Filing date: 2022-08-04
Publication date: 2022-11-01
Anticipated expiration: 2042-08-04
Also published as: CN115252520B

Abstract

The invention discloses a composite plant extract and application thereof. The composite plant extract comprises the following components in percentage by mass: the ginseng extract: 26 to 68 percent; and (3) wolfberry extract: 10% -20%; white fungus extract: 10%. E20 percent; and (3) extracting radix ophiopogonis: 5% -15%; sugarcane root extract: 5% -15%; 1,2-hexanediol: 1% -2%; 1,2-pentanediol: 1% -2%; the ginseng extract, the medlar extract, the tremella extract, the ophiopogon root extract and the sugarcane root extract are all prepared by solid state fermentation, enzymolysis and ultrafiltration. The composite plant extract has the effects of lasting moisture preservation, oxidation resistance, saccharification resistance, skin elasticity improvement and the like, has simple preparation process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Description

Composite plant extract and application thereof

Technical Field

The invention relates to the technical field of skin care, and particularly relates to a composite plant extract and application thereof.

Background

The plant extract is obtained by extracting or processing a plant (whole or a part of the plant) as a raw material with a suitable solvent or method, and can be used in various fields such as medicines, foods, daily chemicals, and the like. At present, one or more plant extracts are added into a plurality of cosmetics, but the following problems are generally existed: 1) Although the single-component plant extract added in the cosmetic formula can exert certain effects, the action mechanism of the plant extract is single, the function is single, and the effect which can be actually exerted is greatly reduced due to the influence of a formula system or certain components in the formula; 2) Plant extracts added in cosmetic formulations are generally difficult to be effectively absorbed by skin, and the effects which can be actually exerted are very limited.

Therefore, the development of the composite plant extract with various effects of lasting moisture preservation, oxidation resistance, saccharification resistance, skin elasticity improvement and the like is of great significance.

Disclosure of Invention

The invention aims to provide a composite plant extract.

The second object of the present invention is to provide a moisturizing composition to which the complex plant extract of the present invention is added.

The technical scheme adopted by the invention is as follows:

a composite plant extract comprises the following components in percentage by mass:

the ginseng extract: 26 to 68 percent;

and (3) wolfberry extract: 10% -20%;

white fungus extract: 10% -20%;

and (3) extracting radix ophiopogonis: 5% -15%;

sugar cane root extract: 5% -15%;

1,2 hexanediol: 1% -2%;

1,2-pentanediol: 1% -2%;

the ginseng extract, the medlar extract, the tremella extract, the ophiopogon root extract and the sugarcane root extract are all prepared by solid state fermentation, enzymolysis and ultrafiltration.

Preferably, the ginseng extract, the medlar extract, the tremella extract, the ophiopogon root extract and the sugarcane root extract are prepared by the following steps:

1) Cleaning and drying the plant raw materials, and then carrying out superfine grinding and screening to obtain plant raw material powder;

2) Dispersing plant raw material powder with water, and autoclaving to obtain solid plant culture medium;

3) Uniformly mixing the activated bacterial liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles and a solid plant culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain plant fermentation liquor;

5) Carrying out enzymolysis on the plant fermentation liquor to obtain an enzymolysis plant fermentation liquor;

6) Inactivating enzyme of the zymolytic plant fermentation liquor, and performing ultrafiltration to obtain the plant extract.

Preferably, the ultrafiltration membrane used for ultrafiltration has a molecular weight cut-off of 1000Da to 2000Da.

Preferably, the ginseng extract is prepared by the following method:

1) Cleaning and drying ginseng, and then carrying out superfine grinding and screening to obtain ginseng powder;

2) Dispersing Ginseng radix powder with water, and autoclaving to obtain solid Ginseng radix culture medium;

3) Uniformly mixing the activated lactobacillus bacterial liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles and a solid ginseng culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain ginseng fermentation liquor;

5) Adjusting the pH value of the ginseng fermentation liquor to 1.0-2.0, adding pepsin for carrying out first enzymolysis, adjusting the pH value to 6.0-7.0, and adding papain for carrying out second enzymolysis to obtain the ginseng fermentation liquor subjected to enzymolysis;

6) Inactivating enzyme of the zymolytic Ginseng radix fermentation liquid, and ultrafiltering to obtain Ginseng radix extract.

Preferably, the drying in step 1) is performed at 50-55 ℃.

Preferably, the screening in step 1) is performed by using a screen of 60-80 meshes.

Preferably, the addition ratio of the ginseng powder and water in the step 2) is 1g.

Preferably, the temperature of the high-pressure sterilization in the step 2) is 121 ℃, and the sterilization time is 20min to 30min.

Preferably, the aging time in the step 3) is 10min to 15min.

Preferably, the fermentation in the step 4) is carried out at 30-35 ℃, and the fermentation time is 3-7 days.

Preferably, the specific operation of adding water for dispersing in the step 4) is as follows: adding the fermented product into water, wherein the mass ratio of the fermented product to the water is 1.5-9.5, mashing, and placing in a constant-temperature water bath for ultrasonic vibration for 1.5-2 h at 20-30 ℃.

Preferably, the centrifugation in the step 4) is carried out at the rotation speed of the centrifuge of 4000r/min to 5000r/min for 15min to 25min.

Preferably, the first enzymolysis in the step 5) is carried out at 35-40 ℃, and the enzymolysis time is 2.5-3 h.

Preferably, the second enzymolysis in the step 5) is carried out at 50-60 ℃, and the enzymolysis time is 2.5-3 h.

Preferably, the enzyme deactivation in the step 6) is carried out at 100-105 ℃, and the enzyme deactivation time is 5-10 min.

Preferably, the ultrafiltration in step 6) is performed under a pressure of 1.5MPa to 2.5 MPa.

Preferably, the medlar extract is prepared by the following method:

1) Cleaning and drying the medlar, and then carrying out superfine grinding and screening to obtain medlar powder;

2) Dispersing fructus Lycii powder with water, and autoclaving to obtain solid fructus Lycii culture medium;

3) Uniformly mixing the activated saccharomycete liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and ageing to obtain embedded strain particles;

4) Mixing the embedded strain particles and a solid medlar culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain medlar fermentation liquor;

5) Adjusting the pH value of the medlar fermentation liquor to 6.0-7.0, and then adding cellulase and pectinase for enzymolysis to obtain the enzymolysis medlar fermentation liquor;

6) Inactivating enzyme of the zymolytic fructus Lycii fermentation liquor, and ultrafiltering to obtain fructus Lycii extract.

Preferably, the drying in step 1) is performed at 50-55 ℃.

Preferably, the screening in the step 1) adopts a screen with 60-80 meshes.

Preferably, the addition ratio of the medlar powder and water in the step 2) is 1g.

Preferably, the aging time in the step 3) is 10min to 15min.

Preferably, the enzymolysis in the step 5) is carried out at 40-50 ℃, and the enzymolysis time is 2.5-3 h.

Preferably, the tremella extract is prepared by the following method:

1) Cleaning and drying tremella, and then carrying out superfine grinding and screening to obtain tremella powder;

2) Dispersing Tremella fuciformis powder with water, and performing autoclaving to obtain a solid Tremella fuciformis culture medium;

3) Uniformly mixing the activated saccharomycete liquid, the activated lactobacillus liquid and the sterilized sodium alginate solution, dripping the mixture into the sterilized calcium chloride solution to form gel beads, and aging to obtain embedded strain particles;

4) Mixing the embedded strain particles with a solid tremella culture medium, fermenting, adding water for dispersion, centrifuging and taking supernatant to obtain tremella fermentation liquor;

5) Adjusting the pH value of the tremella fermentation liquid to 6.0-7.0, and then adding cellulase, pectinase and neutral protease for enzymolysis to obtain an enzymolysis tremella fermentation liquid;

6) And (3) inactivating enzyme of the zymolytic tremella fermentation liquor, and then performing ultrafiltration to obtain the tremella extract.

Preferably, the drying in step 1) is performed at 50-55 ℃.

Preferably, the screening in the step 1) adopts a screen with 60-80 meshes.

Preferably, the addition ratio of the tremella powder and water in the step 2) is 1g.

Preferably, the aging time in the step 3) is 10min to 15min.

Preferably, the ophiopogon japonicus extract is prepared by the following method:

1) Cleaning and drying radix ophiopogonis, and then carrying out superfine grinding and screening to obtain radix ophiopogonis powder;

2) Dispersing radix Ophiopogonis powder with water, and autoclaving to obtain solid radix Ophiopogonis culture medium;

4) Mixing the embedded strain particles and a solid radix ophiopogonis culture medium, fermenting, adding water for dispersion, centrifuging and taking supernate to obtain radix ophiopogonis fermentation liquor;

5) Adjusting the pH value of the radix ophiopogonis fermentation liquor to 6.0-7.0, adding cellulase and pectinase for enzymolysis, obtaining an enzymolyzed radix Ophiopogonis fermentation broth;

6) Inactivating enzyme of the enzymolyzed radix Ophiopogonis fermentation liquid, and ultrafiltering to obtain radix Ophiopogonis extract.

Preferably, the drying in step 1) is performed at 50-55 ℃.

Preferably, the screening in the step 1) adopts a screen with 60-80 meshes.

Preferably, the addition ratio of the wheat and winter flour to the water in the step 2) is 1g.

Preferably, the aging time in the step 3) is 10min to 15min.

Preferably, the enzymolysis in the step 5) is carried out at the temperature of 40-50 ℃, and the enzymolysis time is 2.5-3 h.

Preferably, the sugar cane root extract is prepared by the following method:

1) Cleaning and drying the sugarcane roots, and then carrying out superfine grinding and screening to obtain sugarcane root powder;

2) Dispersing the sugarcane root powder with water, and then performing autoclaving to obtain a solid sugarcane root culture medium;

4) Mixing the embedded strain particles with a solid sugarcane root culture medium, fermenting, adding water for dispersion, and centrifuging to obtain a supernatant to obtain a sugarcane root fermentation liquid;

5) Adjusting the pH value of the sugarcane root fermentation liquid to 6.0-7.0, and then adding cellulase and pectinase for enzymolysis to obtain the enzymolysis sugarcane root fermentation liquid;

6) And (4) inactivating enzyme of the zymolytic sugarcane root fermentation liquor, and then performing ultrafiltration to obtain the sugarcane root extract.

Preferably, the drying in step 1) is performed at 50-55 ℃.

Preferably, the screening in the step 1) adopts a screen with 60-80 meshes.

Preferably, the addition ratio of the sugarcane root powder and water in the step 2) is 1g.

Preferably, the aging time in the step 3) is 10min to 15min.

Preferably, the specific operation of adding water for dispersing in the step 4) is as follows: adding the fermentation product into water, wherein the mass ratio of the fermentation product to the water is 1.5-9.5, mashing, and placing in a constant-temperature water bath for ultrasonic vibration for 1.5-2 h at 20-30 ℃.

Preferably, the centrifugation in the step 4) is carried out at the rotation speed of the centrifugal machine of 4000 r/min-5000 r/min for 15 min-25 min.

Use of the above compound plant extract in cosmetics is provided.

The essence comprises the following components in percentage by mass:

humectant: 7 to 12 percent;

chelating agent: 0.01 to 0.1 percent;

thickening agent: 0.1 to 0.2 percent;

the above composite plant extract: 0.5 to 20 percent;

preservative: 0.2% -1%;

water: 70 to 90 percent.

Preferably, the humectant is at least one of glycerin, butylene glycol and betaine.

Preferably, the chelating agent is at least one of disodium EDTA and calcium citrate.

Preferably, the thickener is at least one of carrageenan and hydrolyzed polysaccharide gum.

Preferably, the preservative is at least 2 of p-hydroxyacetophenone, 1,2-hexanediol, methylparaben and phenoxyethanol.

The essence cream comprises the following components in percentage by mass:

phase A:

humectant: 5% -10%; disodium EDTA: 0.01 to 0.1 percent; water: 60% -80%;

phase B:

emulsifier: 1% -3%; oil ester: 5% -10%;

and C phase:

thickening agent: 1% -2%;

phase D:

the above composite plant extract: 0.5 to 20 percent; preservative: 0.5 to 1.5 percent.

Preferably, the emulsifier is emulsifier MONTANOV 82 (main ingredient: cetearyl alcohol and coco glucoside), emulsifier MONTANOV 202 (main ingredient: arachidyl alcohol, behenyl alcohol and arachidyl glucoside), emulsifier SENSANOV WR (main ingredient: C) ₂₀ ～C ₂₂ Alcohol phosphoric acid esters and C ₂₀ ～C ₂₂ Alcohol).

Preferably, the oil is at least one of caprylic capric triglyceride, polydimethylsiloxane, isononyl isononanoate and mineral oil.

Preferably, the thickener is at least one of thickener SEPINOV EMT 10 (main component: hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer), thickener SEPIPLUS 400 (main component: polyacrylate-13, polyisobutylene and polysorbate-20).

The beneficial effects of the invention are: the composite plant extract has the effects of lasting moisture preservation, oxidation resistance, saccharification resistance, skin elasticity improvement and the like, has simple preparation process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Specifically, the method comprises the following steps:

1) The composite plant extract is prepared from ginseng, medlar, tremella, dwarf lilyturf tuber and sugarcane root through solid state fermentation, enzymolysis and ultrafiltration, so that the extraction rate of active ingredients is maximized, the plant extract containing various active ingredients such as polysaccharide, saponin, vitamin, amino acid, polypeptide and the like is obtained, the molecular weight of each active ingredient in the plant extract is controlled to be below 2000Da through ultrafiltration, the efficient absorption of skin is facilitated, in addition, the synergistic effect of the various plant extracts can be fully exerted by compounding the plant extracts, and the action effect of the composite plant extract can be further highlighted;

2) The composite plant extract has simple production process and low production cost, can be applied to various cosmetics, and is suitable for large-scale industrial application.

Detailed Description

The invention will be further explained and illustrated with reference to specific examples.

Example 1:

a composite plant extract having the composition shown in the following table:

TABLE 1 composition table of a composite plant extract

Raw materials	Mass percent (%)
		Ginseng extract	47
Extract of Lycium barbarum	15
		Tremella extract	15
Radix Ophiopogonis extract	10
		Sugarcane root extract	10
1,2 hexanediol	1.5
		1,2 pentanediol	1.5

Note:

the ginseng extract is prepared by the following method: 1) Cleaning Ginseng radix, oven drying at 55 deg.C, micronizing with micronizer, and sieving with 80 mesh sieve to obtain Ginseng radix powder; 2) Stirring 10g of ginseng powder with 25mL of deionized water, dispersing, placing into an autoclave, and autoclaving at 121 ℃ for 30min to obtain a solid ginseng culture medium; 3) Uniformly mixing activated lactobacillus bacterial liquid and a sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, then dripping the mixture into a sterilized calcium chloride solution with the mass fraction of 4% by using an injector to form gel beads, standing for 10min for aging, filtering, washing the filtered solid with sterile water, and obtaining embedded strain particles; 4) Mixing the embedded strain particles and a solid ginseng culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermentation product into deionized water, wherein the mass ratio of the fermentation product to the deionized water is 1:9, mashing, putting the mixture into a constant-temperature water bath, vibrating for 2 hours at 25 ℃ by ultrasonic waves, centrifuging for 25 minutes at the rotating speed of a centrifuge of 5000r/min, and taking supernatant to obtain ginseng fermentation liquor; 5) Adjusting pH of the Ginseng radix fermentation broth to 1.5, adding pepsin, performing enzymolysis at 37 deg.C for 3 hr, adjusting pH to 6.5, adding papain, and performing enzymolysis at 50 deg.C for 3 hr to obtain an enzymolysis Ginseng radix fermentation broth; 6) Heating the zymolytic ginseng fermentation liquor to 105 ℃, inactivating enzyme for 10min, cooling to 25 ℃, and performing ultrafiltration by using an ultrafiltration membrane with molecular weight cutoff of 2000Da under the condition that the pressure is 2.5MPa to obtain the ginseng extract.

The medlar extract is prepared by the following method: 1) Cleaning fructus Lycii, oven drying at 55 deg.C, micronizing with micronizer, and sieving with 80 mesh sieve to obtain fructus Lycii powder; 2) Stirring and dispersing 10g of medlar powder with 25mL of deionized water, then putting into an autoclave, and carrying out autoclaving at 121 ℃ for 30min to obtain a solid medlar culture medium; 3) Uniformly mixing the activated saccharomycete liquid and a sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, dripping the mixture into a sterilized calcium chloride solution with the mass fraction of 4% by using an injector to form gel beads, standing for 10min for aging, filtering, washing the filtered solid with sterile water, and obtaining embedded strain particles; 4) Mixing the embedded strain particles and a solid medlar culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermentation product into deionized water, wherein the mass ratio of the fermentation product to the deionized water is 1:9, mashing, putting the mixture into a constant-temperature water bath, vibrating for 2 hours at 25 ℃ by ultrasonic waves, centrifuging for 25 minutes at the rotating speed of a centrifugal machine under the condition of 5000r/min, and taking supernatant to obtain medlar fermentation liquor; 5) Adjusting the pH value of the medlar fermentation liquor to 6.5, adding cellulase and pectinase, and carrying out enzymolysis at 50 ℃ for 3 hours to obtain an enzymolysis medlar fermentation liquor; 6) Heating the zymolytic fructus Lycii fermentation broth to 105 deg.C, inactivating enzyme for 10min, cooling to 25 deg.C, and ultrafiltering with ultrafiltration membrane with molecular weight cutoff of 2000Da under pressure of 2.5MPa to obtain fructus Lycii extract.

The tremella extract is prepared by the following method: 1) Cleaning Tremella, oven drying at 55 deg.C, micronizing, and sieving with 60 mesh sieve to obtain Tremella powder; 2) Stirring and dispersing 10g of tremella powder with 25mL of deionized water, then putting into an autoclave, and carrying out autoclaving at 121 ℃ for 30min to obtain a solid tremella culture medium; 3) Uniformly mixing the activated saccharomycete liquid, the activated lactobacillus liquid and a sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1; 4) Mixing the embedded strain particles and a solid tremella culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermentation product into deionized water, mashing the mixture, putting the mixture into constant-temperature water bath, vibrating for 2 hours at 25 ℃ by ultrasonic waves, centrifuging for 25 minutes at the rotating speed of a centrifuge of 5000r/min, and taking supernatant to obtain tremella fermentation liquor; 5) Adjusting the pH value of the tremella fermentation broth to 6.5, adding cellulase, pectinase and neutral protease, and performing enzymolysis at 50 ℃ for 3h to obtain an enzymolysis tremella fermentation broth; 6) Heating the zymolytic tremella fermentation liquor to 105 ℃, inactivating enzyme for 10min, cooling to 25 ℃, and performing ultrafiltration by using an ultrafiltration membrane with molecular weight cutoff of 2000Da under the condition that the pressure is 2.5MPa to obtain the tremella extract.

The radix ophiopogonis extract is prepared by the following method: 1) Cleaning radix Ophiopogonis, oven drying at 55 deg.C, micronizing, and sieving with 60 mesh sieve to obtain radix Ophiopogonis powder; 2) Stirring and dispersing 10g of dwarf lilyturf tuber powder with 25mL of deionized water, then putting into an autoclave, and carrying out autoclaving at 121 ℃ for 30min to obtain a solid dwarf lilyturf tuber culture medium; 3) Uniformly mixing activated lactobacillus bacterial liquid and a sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, then dripping the mixture into a sterilized calcium chloride solution with the mass fraction of 4% by using an injector to form gel beads, standing for 10min for aging, filtering, washing the filtered solid with sterile water, and obtaining embedded strain particles; 4) Mixing the embedded strain particles and a solid radix ophiopogonis culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermentation product into deionized water, mashing the fermentation product and the deionized water at a mass ratio of 1:9, putting the mixture into a constant-temperature water bath, vibrating for 2 hours at 25 ℃ by ultrasonic waves, centrifuging for 25 minutes at the rotating speed of a centrifuge of 5000r/min, and taking supernatant to obtain radix ophiopogonis fermentation liquor; 5) Adjusting the pH value of the radix ophiopogonis fermentation liquor to 6.5, adding cellulase and pectinase, and carrying out enzymolysis at 50 ℃ for 3h to obtain an enzymolysis radix ophiopogonis fermentation liquor; 6) Heating the zymolytic radix Ophiopogonis fermentation broth to 105 deg.C, inactivating enzyme for 10min, cooling to 25 deg.C, and ultrafiltering with ultrafiltration membrane with molecular weight cutoff of 2000Da under pressure of 2.5MPa to obtain radix Ophiopogonis extract.

The sugarcane root extract is prepared by the following method: 1) Cleaning sugarcane roots, drying at 55 ℃, carrying out superfine grinding by using a superfine grinder, and screening by using a 60-mesh screen to obtain sugarcane root powder; 2) Stirring and dispersing 10g of sugarcane root powder with 25mL of deionized water, then putting into an autoclave, and carrying out autoclaving at 121 ℃ for 30min to obtain a solid sugarcane root culture medium; 3) Uniformly mixing activated lactobacillus bacterial liquid and a sterilized sodium alginate solution with the mass fraction of 3% according to the volume ratio of 1:5, then dripping the mixture into a sterilized calcium chloride solution with the mass fraction of 4% by using an injector to form gel beads, standing the gel beads for 10min for aging, filtering, washing the filtered solid with sterile water, and obtaining embedded strain particles; 4) Mixing the embedded strain particles and a solid sugarcane root culture medium, putting the mixture into a constant-temperature vibration incubator, fermenting for 5 days at 35 ℃, adding the fermentation product into deionized water, mashing the mixture, putting the mixture into constant-temperature water bath, vibrating for 2 hours at 25 ℃ by ultrasonic waves, centrifuging for 25 minutes at the rotating speed of a centrifuge of 5000r/min, and taking supernatant to obtain sugarcane root fermentation liquid; 5) Adjusting the pH value of the sugarcane root fermentation liquid to 6.5, adding cellulase and pectinase, and carrying out enzymolysis at 50 ℃ for 3h to obtain an enzymolysis sugarcane root fermentation liquid; 6) Heating the zymolytic sugarcane root fermentation liquor to 105 ℃, inactivating the enzyme for 10min, cooling to 25 deg.C, and ultrafiltering with ultrafiltration membrane with molecular weight cutoff of 2000Da under pressure of 2.5MPa to obtain extract of sugarcane root.

Example 2:

a composite plant extract having the composition shown in the following table:

TABLE 2 composition table of a composite plant extract

Raw materials	Mass percent (%)
		Ginseng extract	49
Extract of Lycium barbarum	13
		Tremella extract	15
Radix Ophiopogonis extract	5
		Sugarcane root extract	15
1,2 hexanediol	2
		1,2 pentanediol	1

Note: the preparation methods of the ginseng extract, the wolfberry extract, the tremella extract, the ophiopogon root extract and the sugarcane root extract are the same as in example 1.

Example 3:

a composite plant extract having the composition shown in the following table:

TABLE 3 composition table of a composite plant extract

Raw materials	Mass percent (%)
		Ginseng extract	37
Extract of Lycium barbarum	20
		Tremella extract	20
Radix Ophiopogonis extract	10
		Sugarcane root extract	10
1,2 hexanediol	1.5
		1,2 pentanediol	1.5

Example 4:

a composite plant extract having the composition shown in the following table:

TABLE 4 composition table of a composite plant extract

Raw materials	Mass percent (%)
		Ginseng extract	27
Extract of Lycium barbarum	20
		Tremella extract	20
Radix Ophiopogonis extract	15
		Sugarcane root extract	15
1,2 hexanediol	1
		1,2 pentanediol	2

Comparative example 1:

a composite plant extract having the composition shown in the following table:

TABLE 5 composition table of a composite plant extract

Starting materials	Mass percent (%)
		Ginseng extract	47
Extract of Lycium barbarum	15
		Tremella extract	15
Ophiopogon japonicus extract	10
		Sugarcane root extract	10
1,2 hexanediol	1.5
		1,2 pentanediol	1.5

Note:

the ginseng extract is prepared by the following method: 1) Cleaning Ginseng radix, oven drying at 55 deg.C, micronizing with micronizer, and sieving with 80 mesh sieve to obtain Ginseng radix powder; 2) Stirring and dispersing 10g of ginseng powder with 90mL of deionized water, soaking for 1h, decocting at 80 ℃ for 1h, filtering, adding filter residues into 90mL of deionized water, decocting at 80 ℃ for 1h, filtering, combining the filtrates, concentrating to 10mL, centrifuging for 25min at the rotation speed of a centrifuge of 5000r/min, taking the supernatant, filtering and sterilizing to obtain the ginseng extract.

The preparation method of the medlar extract, the tremella extract, the ophiopogon root extract and the sugarcane root extract is the same as that of the ginseng extract.

Comparative example 2:

ginseng extract (prepared as in example 1).

Application example 1:

an essence having the composition shown in the following table:

TABLE 6 composition table of essence

Raw materials	Mass percent (%)
		Glycerol	6.0
Butanediol	4.0
		EDTA disodium salt	0.02
Hydrolyzed polysaccharide gums	0.1
		Composite plant extract of example 1	3.0
P-hydroxyacetophenone	0.5
		1,2 hexanediol	0.5
Deionized water	85.88

The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, disodium EDTA and hydrolyzed polysaccharide gum in deionized water, heating to 80 deg.C, keeping the temperature for 20min, cooling to 45 deg.C, adding the composite plant extract of example 1 and transparent p-hydroxyacetophenone and 1,2-hexanediol, and mixing to obtain essence.

Application example 2:

an essence having the composition shown in the following table:

TABLE 7 composition table of essence

The preparation method of the essence comprises the following steps: dissolving glycerol, betaine, EDTA disodium and carrageenan in deionized water, heating to 80 ℃, keeping the temperature for 20min, cooling to 45 ℃, adding the composite plant extract of example 1 and 1,2-hexanediol, methylparaben and phenoxyethanol which are preheated and mixed to be transparent, and uniformly mixing to obtain the essence.

Application example 3:

an essence cream, the composition of which is shown in the following table:

table 8 composition table of essence cream

The preparation method of the essence cream comprises the following steps: respectively mixing and stirring the phase A raw material and the phase B raw material uniformly, heating to 85 ℃, then adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C, homogenizing for 6min again, keeping the temperature for 20min, cooling to 45 ℃, adding the composite plant extract of the embodiment 1 in the phase D and the transparent p-hydroxyacetophenone and 1,2-hexanediol which are heated and dissolved in advance, and uniformly mixing to obtain the essence cream.

Application example 4:

an essence cream, the composition of which is shown in the following table:

TABLE 9 composition table of essence cream

The preparation method of the essence cream comprises the following steps: respectively mixing and stirring the phase A raw material and the phase B raw material uniformly, heating to 85 ℃, then adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C raw material, homogenizing for 6min, keeping the temperature for 20min, cooling to 45 ℃, adding the composite plant extract of the embodiment 1 in the phase D and 1,2-hexanediol, methylparaben and phenoxyethanol which are preheated and dissolved to be transparent, and uniformly mixing to obtain the essence cream.

Comparative application example 1:

an essence having the composition shown in the following table:

TABLE 10 composition table of essence

The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, disodium EDTA and hydrolyzed polysaccharide gum in deionized water, heating to 80 deg.C, keeping the temperature for 20min, cooling to 45 deg.C, adding the composite plant extract of comparative example 1 and transparent p-hydroxyacetophenone and 1,2-hexanediol, and mixing to obtain essence.

Comparative application example 2:

an essence having the composition shown in the following table:

TABLE 11 composition table of essence

Starting materials	Mass percent (%)
		Glycerol	6.0
Butanediol	4.0
		EDTA disodium salt	0.02
Hydrolyzed polysaccharide acids	0.1
		Ginseng extract of comparative example 2	3.0
P-hydroxyacetophenone	0.5
		1,2 hexanediol	0.5
Deionized water	85.88

The preparation method of the essence comprises the following steps: dissolving glycerol, butanediol, disodium EDTA and hydrolyzed polysaccharide gum in deionized water, heating to 80 deg.C, keeping the temperature for 20min, cooling to 45 deg.C, adding the Ginseng radix extract of comparative example 2 and transparent p-hydroxyacetophenone and 1,2-hexanediol, and mixing to obtain essence.

Comparative application example 3:

an essence cream, the composition of which is shown in the following table:

TABLE 12 composition table of essence cream

The preparation method of the essence cream comprises the following steps: respectively mixing and stirring the phase A raw material and the phase B raw material uniformly, heating to 85 ℃, then adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C raw material, homogenizing for 6min, keeping the temperature for 20min, cooling to 45 ℃, adding the composite plant extract of the comparative example 1 in the phase D and 1,2-hexanediol, methylparaben and phenoxyethanol which are preheated and dissolved to be transparent, and uniformly mixing to obtain the essence cream.

Comparative application example 4:

an essence cream, the composition of which is shown in the following table:

TABLE 13 composition table of essence cream

The preparation method of the essence cream comprises the following steps: respectively mixing and stirring the phase A raw material and the phase B raw material uniformly, heating to 85 ℃, then adding the phase B raw material into the phase A raw material, homogenizing and emulsifying for 6min, adding the phase C raw material, homogenizing for 6min again, keeping the temperature for 20min, cooling to 45 ℃, adding the ginseng extract of the comparative example 2 in the phase D and 1,2-hexanediol, methylparaben and phenoxyethanol which are preheated and dissolved to be transparent, and uniformly mixing to obtain the essence cream.

Comparative application example 5:

the Obes moisturizing and repairing stock solution (Meichi cosmetics Co., ltd., guangzhou city).

Comparative application example 6:

obes moisturizing cream (Meichi cosmetics Co., ltd., guangzhou city).

And (3) performance testing:

1) Measurement of skin moisture content:

and (4) testing standard: QB/T4256-2011 guide for evaluating moisturizing efficacy of cosmetics.

Test object(s): 200 volunteers (male and female are not limited, except women in gestation period and lactation period) with the age of 18-60 years have healthy skin and no history of skin allergy, the test sites were not enrolled in other clinical trials for the last three months, and 200 volunteers were randomized into 8 groups (scored as group a, group B, group C, group D, group E, group F, group G, and group H), with 25 persons per group.

And (3) testing environment: the temperature is 20-22 ℃, and the relative humidity is 40-60%, so that real-time dynamic detection is carried out.

The test method comprises the following steps:

a) The tested part can not be used with any product 2-3 days before testing, can not contact water for 1-3 hours, a mark of 3cm multiplied by 3cm is made on the inner side of the front arm, the tested part is wiped by dry facial tissue before testing, and then the part is statically seated for 20min;

b) 0h (blank test): measuring the test area by using MoistureMeter SC cuticle water content measuring instrument, measuring for 3 times in parallel, and measuring the sample content according to 2.0mg/cm ² ±0.1mg/cm ² The amount of the coating is used for single coating, and the actual sample coating amount is recorded;

c) 1h (experimental test): measuring a test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and performing parallel measurement for 3 times; 2h (experimental test): measuring a test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and performing parallel measurement for 3 times; 4h (experimental testing): measuring a test area by using a MoistureMeter SC stratum corneum water content measuring instrument, and performing parallel measurement for 3 times;

d) The group a used the essence of application example 1, the group B used the essence of application example 4, the group C used the essence of comparative application example 1, the group D used the essence of comparative application example 3, the group E used the essence of comparative application example 2, the group F used the essence of comparative application example 4, the group G used the obeys moisturizing lotion of comparative application example 5, and the group H used the obeys moisturizing lotion of comparative application example 6.

And (3) testing indexes:

a) The larger the value of the water content of the horny layer is, the higher the water content of the horny layer of the skin is;

b) Percent change (%) = (n hours value-0 hours value)/0 hours value × 100%;

c) Total effective rate (%) = effective number/total number × 100%.

And (3) test result calculation:

a) Performing descriptive statistics on the measured values of all the test areas, wherein the descriptive statistics comprise an average value, a standard deviation, a minimum value, a median value and a maximum value;

b) And (3) analyzing by using SPSS 22.0, comparing the difference before and after the test by applying a matched sample T test according to normal distribution, comparing the difference without applying a rank sum test according to normal distribution, wherein the difference is significant if P is less than or equal to 0.05, and the difference is not significant if P is greater than 0.05, wherein a statistical method adopts two-tail detection, and the detection level alpha =0.05.

The test results are shown in tables 14 to 29:

TABLE 14A comparison of stratum corneum hydration results for subjects in the placebo and test zones (units: a.u.)

TABLE 15 results of percentage change in stratum corneum water content of A group subjects

Period of time	1h	2h	4h
				Percent change (%)	141.18	135.40	139.06

Table 16B group subjects the results of comparing the stratum corneum hydration in the placebo and test zones (units: a.u.)

TABLE 17 results of percentage change in stratum corneum Water content of B group subjects

Period of time	1h	2h	4h
				Percentage change (%)	139.20	133.74	136.09

TABLE 18C comparison of stratum corneum hydration results for subjects in the placebo and test zones (units: a.u.)

TABLE 19 results of percentage change in stratum corneum water content of C group subjects

Period of time	1h	2h	4h
				Percent change (%)	76.78	69.49	47.89

TABLE 20D comparison of stratum corneum hydration results (units: a.u.) for subjects in the placebo and test zones

TABLE 21 results of percentage change in stratum corneum water content in D group subjects

Period of time	1h	2h	4h
				Percentage change (%)	76.30	61.41	49.75

TABLE 22E comparison of stratum corneum hydration results (units: a.u.) for the blank control and test areas of subjects

TABLE 23 results of percentage change in stratum corneum water content in subjects in E group

Period of time	1h	2h	4h
				Percent change (%)	78.03	70.69	48.94

Table 24F group subjects the results of stratum corneum moisture content comparison (units: a.u.) between the blank control zone and the test zone

TABLE 25 results of percentage change in water content in stratum corneum of F group subjects

Period of time	1h	2h	4h
				Percent change (%)	71.98	60.27	47.05

TABLE 26G group subjects blank control zone compared to test zone stratum corneum hydration results (units: a.u.)

TABLE 27 results of percentage change in stratum corneum water content of G group subjects

Period of time	1h	2h	4h
				Percent change (%)	85.45	99.69	40.18

TABLE 28H comparison of stratum corneum hydration results for subjects in the placebo and test zones (units: a.u.)

TABLE 29 results of percentage change in water content in stratum corneum of H group subjects

Period of time	1h	2h	4h
				Percent change (%)	87.69	99.65	45.47

From tables 14 to 29, it is clear that: by using SPSS 22.0 for analysis, the difference between the groups before and after the test is in normal distribution, and the difference between the blank control areas of the group A and the group B and the difference between the tested areas have significance difference of less than or equal to 0.05 when the difference is 1h, 2h and 4h, which indicates that the significance of the water content of the horny layer is increased and the tested sample has the moisturizing effect compared with the test sample without any product; the difference between the blank control area difference and the tested area difference of the group C, the group D, the group E and the group F is not more than 0.05 at 1H, and is more than 0.05 at 2H and 4H, which indicates that the tested sample has the significant difference before 1H in the water content of the horny layer, the tested sample has the moisturizing effect, and has no significant difference after 2H, and has no moisturizing effect, and the group G, the group H, the group 1H and the group 2H have the P not more than 0.05, and has the P more than 0.05 at 4H, which indicates that the tested sample has the moisturizing effect before 2H, and has no moisturizing effect after 4H.

In conclusion, the essence of application example 1 and the essence of application example 4 have more remarkable moisturizing effect and longer duration of the moisturizing effect in the test, which shows that the composite plant extract of the present invention has more prominent moisturizing effect and longer moisturizing time when being used in cosmetics, compared to the composite plant extract and the single ginseng extract prepared by the common extraction method.

2) Skin elasticity test:

test object(s): 80 volunteers (male and female are not limited, except for pregnant and lactating women) aged 18-60 years have healthy skin and no allergic history of skin diseases, tested parts of the patients in the last three months do not participate in other clinical tests, and the 80 volunteers are randomly divided into 8 groups (marked as A group, B group, C group, D group, E group, F group, G group and H group), and each group comprises 10 persons.

The test method comprises the following steps: the group A uses the essence of the application example 1, the group B uses the essence of the application example 4, the group C uses the essence of the comparative application example 1, the group D uses the essence of the comparative application example 3, the group E uses the essence of the comparative application example 2, the group F uses the essence of the comparative application example 4, the group G uses the Obes moisturizing and repairing stock solution of the comparative application example 5, the group H uses the Obes moisturizing and repairing cream of the comparative application example 6, the groups H and the groups H are continuously used for 4 weeks, and the parts to be measured are respectively measured by a skin elasticity tester Revisometer RV600 before use, after 1 week, after 2 weeks and after 4 weeks.

The test results are shown in table 30:

TABLE 30 skin elasticity test results

Note: percent (%) elastic change (= Rn used n weeks-Rn used)/Rn used x 100%.

As can be seen from table 30: after 4 weeks, the skin elasticity index of the volunteer who uses the essence of application example 1 and the essence of application example 4 rises most obviously, and the skin elasticity is greatly improved, which shows that compared with the composite plant extract and the single ginseng extract which are prepared by the common extraction method, the composite plant extract of the invention is easier to permeate and absorb and is used in the cosmetics to increase the skin elasticity and the water content of the horny layer.

3) Oxidative stress testing:

and (3) checking the principle: oxidative stress is one of the important causes of skin cell aging damage, and excessive production of free radicals by oxygen metabolism of the body leads to skin aging and induces the formation of skin wrinkles. Vitamin C (ascorbic acid) is an effective radical scavenger, and it is converted into dehydroascorbic acid by stepwise supply of electrons to scavenge radicals such as DPPH.

The test method comprises the following steps:

a) Test samples were divided into 8 groups: group a used the essence of application example 1, group B used the essence of application example 4, group C used the essence of comparative application example 1, group D used the essence of comparative application example 3, group E used the essence of comparative application example 2, group F used the essence of comparative application example 4, group G used the obeys moisturizing lotion of comparative application example 5, group H used the obeys moisturizing lotion of comparative application example 6;

b) Accurately weighing a test sample and vitamin C, respectively adding a proper amount of absolute ethyl alcohol for dilution, shaking up, preparing a series of concentrations of a vitamin C solution by using the absolute ethyl alcohol for standby, respectively mixing the test sample solution and the vitamin C solution with the series of concentrations with a DPPH solution, standing for 30min in a dark place at room temperature, measuring absorbance at 515nm, taking the vitamin C as a positive control, taking 2 times for each group of samples, and taking an average value.

The test results are shown in table 31:

TABLE 31 measurement of DPPH radical scavenging Rate of test samples

As can be seen from table 31: compared with other control samples, the essence of application example 1 and the essence of application example 4 have a much higher DPPH free radical scavenging rate, namely, the application example 1 and the application example 4 have the strongest antioxidant capacity, which shows that the composite plant extract of the invention has stronger antioxidant capacity compared with the composite plant extract prepared by a common extraction method and a single ginseng extract.

4) Anti-glycation assay:

the test method comprises the following steps:

a) Dividing test samples into 8 groups, wherein the group A uses the essence of the application example 1, the group B uses the essence of the application example 4, the group C uses the essence of the comparative application example 1, the group D uses the essence of the comparative application example 3, the group E uses the essence of the comparative application example 2, the group F uses the essence of the comparative application example 4, the group G uses the Obes moisturizing and protecting original liquid of the comparative application example 5, the group H uses the Obes moisturizing and nourishing cream of the comparative application example 6, and the 8 groups of samples are respectively diluted into aqueous solutions with the mass ratio of 10%;

b) Adding 1mL of sample and 1mL of fructose solution (1.5 mol/L) into a test tube under aseptic condition, incubating for 2h in a constant temperature incubator at 37 ℃ after uniformly mixing, adding 1mL of BSA (bovine serum albumin) solution (30 mg/mL), dissolving the reactants by using a phosphate buffer solution containing 0.1% of sodium azide, taking an aminoguanidine substitute sample with the same mass concentration as a positive representative, taking a phosphate buffer solution substitute sample as a blank control, placing the samples in the incubator at a fixed temperature at 37 ℃ for reacting for 6d, measuring the fluorescence intensity of the samples under the conditions that the excitation wavelength is 370nm and the emission wavelength is 440nm, measuring each group twice, averaging, and calculating the formation inhibition rate R of AGEs according to the formula: r (%) = (1-F) _A /F _B ) X 100%, wherein F _A Fluorescence intensity for each sample set, F _B Fluorescence intensity of blank group.

The test results are shown in table 32:

TABLE 32 inhibition of fluorescent AGEs production by test samples

As can be seen from table 32: the essence of application example 1 and the essence of application example 4 have the highest inhibition rate on the generation of fluorescent AGEs, that is, the anti-saccharification capacity of application example 1 and application example 4 is the strongest, which shows that the anti-saccharification capacity of the composite plant extract is more outstanding than that of a composite plant extract and a single ginseng extract prepared by a common extraction method.

5) Skin feel test:

test objects: 80 volunteers (male and female are not limited, except for pregnant and lactating women) aged 18-60 years have healthy skin and no history of skin disease allergy, and the 80 volunteers are randomly divided into 8 groups (marked as A group, B group, C group, D group, E group, F group, H group and G group), and each group has 10 persons.

The test method comprises the following steps: the group A uses the essence of the application example 1, the group B uses the essence of the application example 4, the group C uses the essence of the comparative application example 1, the group D uses the essence of the comparative application example 3, the group E uses the essence of the comparative application example 2, the group F uses the essence of the comparative application example 4, the group G uses the Obes moisturizing and protecting stock solution of the comparative application example 5, the group H uses the Obes moisturizing and moisturizing cream of the comparative application example 6, the groups are continuously used for 4 weeks, each group is measured twice, and the average value of each group is taken.

The test results are shown in table 33:

TABLE 33 skin feel test results

Note: the score of each item is 0-10, with 0 being the worst and 10 being the best.

As can be seen from table 33: the essence of application example 1 and the essence cream of application example 4 have the highest score, which shows that the composite plant extract of the invention has good spreadability, easy absorption and no stickiness when being used in cosmetics compared with the composite plant extract prepared by a common extraction method and a single ginseng extract, and is more easily accepted and favored by consumers.

In addition, by performing the same test, it was found that: the effects of the composite plant extracts of examples 2 to 4 in terms of moisturizing, oxidation resistance, anti-glycation, skin elasticity improvement, etc. are very similar to those of the composite plant extract of example 1.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such modifications are intended to be included in the scope of the present invention.

Claims

1. The composite plant extract is characterized by comprising the following components in percentage by mass:

the ginseng extract: 26 to 68 percent;

and (3) wolfberry extract: 10% -20%;

white fungus extract: 10% -20%;

and (3) extracting radix ophiopogonis: 5% -15%;

sugarcane root extract: 5% -15%;

1,2 hexanediol: 1% -2%;

1,2-pentanediol: 1% -2%;

2. The composite plant extract as claimed in claim 1, wherein: the molecular weight cut-off of the ultrafiltration membrane used in the ultrafiltration is 1000 Da-2000 Da.

3. The composite plant extract as claimed in claim 1 or 2, wherein: the ginseng extract is prepared by the following method:

1) Cleaning and drying ginseng, carrying out superfine grinding and screening to obtain ginseng powder;

4. The composite plant extract as claimed in claim 1 or 2, wherein: the medlar extract is prepared by the following method:

5. The composite plant extract as claimed in claim 1 or 2, wherein: the tremella extract is prepared by the following method:

2) Dispersing Tremella powder with water, and autoclaving to obtain solid Tremella culture medium;

6. The composite plant extract as claimed in claim 1 or 2, wherein: the radix ophiopogonis extract is prepared by the following method:

1) Cleaning and drying radix Ophiopogonis, micronizing and sieving to obtain radix Ophiopogonis powder;

5) Adjusting the pH value of the radix ophiopogonis fermentation liquor to 6.0-7.0, and then adding cellulase and pectinase for enzymolysis to obtain the enzymolyzed radix ophiopogonis fermentation liquor;

7. The composite plant extract as claimed in claim 1 or 2, wherein: the sugarcane root extract is prepared by the following method:

1) Cleaning and drying sugarcane roots, and then carrying out superfine grinding and screening to obtain sugarcane root powder;

5) Adjusting the pH value of the sugarcane root fermentation liquor to 6.0-7.0, and then adding cellulase and pectinase for enzymolysis to obtain the enzymolysis sugarcane root fermentation liquor;

8. Use of the complex plant extract of any one of claims 1 to 7 in cosmetics.

9. The essence is characterized by comprising the following components in percentage by mass:

humectant: 7 to 12 percent;

chelating agent: 0.01 to 0.1 percent;

thickening agent: 0.1 to 0.2 percent;

the complex plant extract of any one of claims 1 to 7: 0.5 to 20 percent;

preservative: 0.2% -1%;

water: 70 to 90 percent.

10. The essence cream is characterized by comprising the following components in percentage by mass:

phase A:

humectant: 5% -10%; disodium EDTA: 0.01 to 0.1 percent; water: 60% -80%;

phase B:

emulsifier: 1% -3%; oil ester: 5% -10%;

and C phase:

thickening agent: 1% -2%;

phase D:

the complex plant extract of any one of claims 1 to 7: 0.5 to 20 percent; preservative: 0.5 to 1.5 percent.