CN111603404B - Skin care essence containing cell extracting solution and preparation method thereof - Google Patents
Skin care essence containing cell extracting solution and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a skin care essence containing a cell extracting solution and a preparation method thereof, wherein the skin care essence comprises the following components in parts by weight: 10-15 parts of purple sweet potato cell extracting solution, 0.5-1 part of ginseng extract, 0.25-0.5 part of peony root extract, 0.08-0.2 part of mulberry leaf extract, 2-4 parts of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickening agent, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water; the invention takes the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract as main materials, and is supplemented with the humectant, the thickener and the like, and the compatibility and the scientific proportion are reasonable, so that different effective active ingredients generate the synergistic interaction effect, the product has excellent effects of removing hydroxyl free radicals, enhancing skin elasticity, delaying senility and supplementing skin moisture, and the preparation process is simple and is suitable for industrial production.
Description
Technical Field
The invention relates to a skin care essence, in particular to a skin care essence containing a cell extracting solution and a preparation method thereof, and belongs to the technical field of cosmetics.
Background
With the age, the water loss in the skin is obvious, the water and oil are unbalanced, and the problems of acne, roughness, wrinkles and the like are easy to occur; meanwhile, the gradually deteriorating environment increases the content of pollutants in the air, and the skin is exposed to the air for a long time and is damaged by the pollutants in the environment, so that the skin has a phenomenon of lipid peroxidation. With the improvement of living standard of people, the functional requirements on skin care products are higher and higher. The essence is a skin care product added with concentrated high-nutrition components, such as plant extracts, ceramide, squalane and the like, and has the effects of resisting aging, resisting wrinkles, preserving moisture, whitening, removing spots and the like according to the pertinence of the formula. The source of the functional components is mainly divided into chemical and plant types, wherein the natural plant type has the characteristics of naturalness, mildness, no toxic or side effect and the like, is well liked by consumers and has wide market prospect.
At present, application research for increasing stem cell active factors in skin care essence is carried out, but no report that plant cell extract is used for producing skin care essence is found.
Disclosure of Invention
The invention aims to provide a skin care essence containing a cell extracting solution, which has the effects of resisting aging, resisting wrinkles and moisturizing.
The invention also aims to provide a preparation method of the skin care essence containing the cell extracting solution, which is simple to prepare and can be used for industrial production.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a skin care essence containing cell extract comprises the following components in parts by weight: 10-15 parts of purple sweet potato cell extract, 2-4 parts of ginseng extract, 0.5-1 part of peony root extract, 0.25-0.5 part of mulberry leaf extract, 0.08-0.2 part of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickener, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water.
Preferably, the skin care essence containing the cell extracting solution comprises the following components in parts by weight: 12 parts of purple sweet potato cell extracting solution, 3 parts of ginseng extract, 0.8 part of peony root extract, 0.4 part of mulberry leaf extract, 0.15 part of tremella polysaccharide, 0.5 part of nicotinamide, 0.3 part of tocopherol, 0.8 part of trehalose, 1.15 parts of betaine, 4 parts of glycerol, 4 parts of dipropylene glycol, 0.45 part of thickening agent, 0.3 part of triethanolamine, 0.1 part of chelating agent and 78 parts of water.
Preferably, the thickening agent is one or more of carbomer and xanthan gum.
Preferably, the chelating agent is disodium EDTA.
Preferably, the water is pure water with the conductivity less than or equal to 2 mu S/cm.
Further, the skin care essence containing the cell extracting solution further comprises one or more of an aromatic, a toner and a preservative.
The invention also provides a preparation method of the skin care essence containing the cell extracting solution, which comprises the following steps:
(1) centrifuging the purple sweet potato cell culture solution, removing the precipitate, freezing and crushing the supernatant at ultralow temperature by using liquid nitrogen to obtain purple sweet potato cell powder;
(2) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing for five times at-80 ℃, centrifuging at 3-5 ℃, taking supernate, namely purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;
(3) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, thickening agent and chelating agent into an emulsifying pot, stirring, heating to 85-90 deg.C, stirring, and sterilizing at constant temperature for 20-25 min;
(4) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 42-48 deg.C, adding accurately weighed purple sweet potato cell extractive solution, Ginseng radix extract, peony root extract, folium Mori extract, Tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring;
(5) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.
Preferably, the extraction method of the purple sweet potato cell culture solution is as follows: taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 1.5-2.5, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the temperature of 50-70 ℃ for 60-120min, wherein the addition amount of the alpha-amylase is 0.01-0.09% of the weight of the purple sweet potato pulp raw material, and the addition amount of the glucoamylase is 0.01-0.05% of the weight of the purple sweet potato pulp raw material, so as to prepare a purple sweet potato saccharification liquid; inoculating 6-12% yeast seed culture solution at volume ratio, culturing at 25-30 deg.C for 3-4 days with pH of 3.0-3.5, and filtering after fermentation.
Preferably, in step (1), the centrifugation conditions are: the rotating speed is 3000-.
Preferably, in the step (2), the centrifugation conditions are as follows: the rotating speed is 8000-.
The pharmacological mechanism analysis of each active component in the formula of the invention is as follows:
purple sweet potato cell extracting solution: rich in natural anthocyanins, has hydroxyl radical scavenging activity, and can inhibit elastase, protect collagen protein and improve skin elasticity;
the ginseng extract: delaying skin aging, preventing skin dryness and dehydration, increasing skin elasticity, protecting skin luster, tendering, preventing and reducing skin wrinkle, and inhibiting melanin reduction to make skin white and smooth.
Peony root extract: the skin care product is rich in amino acids, mineral substances, various vitamins, zinc, selenium and other trace elements required by a human body, has a strong relieving effect, and can reduce the activity of melanin in skin keratinocytes so as to help inhibit the generation of melanin; has antibacterial and antiinflammatory effects; the cream stimulates the lymphatic circulation of the skin, makes the skin glossy, improves the skin quality, and can make the skin slowly volatilize dull and rough, and make the skin bright and moist after being continuously used.
And (3) mulberry leaf extract: contains various physiological active substances such as folium Mori flavone, folium Mori polyphenol, folium Mori polysaccharide, etc., and has effects of inhibiting elastase, delaying aging, inhibiting tyrosinase activity, and whitening skin.
White fungus polysaccharide: the skin care product can activate epidermal cells, repair photodamaged skin, accelerate skin regeneration, and form a uniform film with a certain thickness on the surface of the skin so that the skin has good smooth and moist feeling;
nicotinamide: the skin-care product can effectively reduce the generation of melanin, accelerate the metabolism of melanin horny cells, promote the synthesis of skin epidermal protein and greatly improve the defense force of skin;
tocopherol is a hydrolysate of vitamin E, is one of the most main antioxidants, has fat solubility, can protect skin, enable the skin to have elasticity, stabilize the protein active structure of a cell membrane, promote the normal development of muscle, keep the elasticity of the skin and enable the skin and the body to keep vitality; the skin cell entering the skin cell can directly help the skin to resist the invasion of free radicals, ultraviolet rays and pollutants, and prevent the skin from losing elasticity until aging due to some chronic or recessive injuries.
Trehalose, betaine, glycerol and dipropylene glycol are used as moisturizers, and can improve the moisture content and the moisture retention of the skin.
Thickening agent: has effects in thickening and improving skin feeling.
Triethanolamine: and the pH regulator is used for regulating the pH value of the system.
Chelating agent: chelating the metal ions.
Compared with the prior art, the invention has the following beneficial effects:
1. the essence disclosed by the invention is added with the purple sweet potato cell extracting solution, and the cells are crushed at ultralow temperature by adopting liquid nitrogen, so that the biological activity of substances in the cells is retained to the greatest extent, the high cell breakage rate is achieved, the anthocyanin is completely released, the permeability is strong, the renewal and growth of the cells of an organism are promoted, and the activity of skin cells is improved.
2. The invention takes the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract as main materials, and is supplemented with the humectant, the thickener and the like, and the compatibility and the scientific proportion are reasonable, so that different effective active ingredients generate the synergistic interaction effect, and the product has excellent effects of removing hydroxyl free radicals, enhancing the skin elasticity, delaying the aging and supplementing the skin moisture.
3. The essence disclosed by the invention is mild and non-irritant to skin, good in safety, simple in preparation process and suitable for industrial production.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1
A skin care essence containing cell extract comprises the following components in parts by weight: 12 parts of purple sweet potato cell extracting solution, 3 parts of ginseng extract, 0.8 part of peony root extract, 0.4 part of mulberry leaf extract, 0.15 part of tremella polysaccharide, 0.5 part of nicotinamide, 0.3 part of tocopherol, 0.8 part of trehalose, 1.15 parts of betaine, 4 parts of glycerol, 4 parts of dipropylene glycol, 0.45 part of carbomer, 0.3 part of triethanolamine, 0.1 part of disodium EDTA and 78 parts of water.
The preparation method of the skin care essence containing the cell extracting solution comprises the following steps:
(1) taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 2, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the enzymolysis temperature of 60 ℃ for 90min, wherein the addition amount of the alpha-amylase is 0.06% of the weight of the raw material of the purple sweet potato pulp, and the addition amount of the glucoamylase is 0.03% of the weight of the raw material of the purple sweet potato pulp, so as to prepare a purple sweet potato saccharification liquid; inoculating yeast from the inclined plane into wort culture medium with mass ratio of soluble solid of 6.0%, and culturing at 28-30 deg.C and 150r/min for 24 hr; inoculating 8% yeast seed culture solution according to volume ratio, culturing at 25 deg.C for 4 days with pH value of 3.0, and filtering after fermentation;
(2) centrifuging the purple sweet potato cell culture solution at 4000r/min for 10min, removing the precipitate, and freezing and crushing the supernatant at ultralow temperature by liquid nitrogen to obtain purple sweet potato cell powder;
(3) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing at-80 ℃ for five times, centrifuging at 3-5 ℃ of 10000r/min for 15min, taking supernatant fluid which is purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;
(4) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, a thickening agent and a chelating agent into an emulsifying pot, stirring, heating to 85 ℃, stirring, and sterilizing at constant temperature for 20 min;
(5) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 45 ℃, then adding accurately weighed purple sweet potato cell extract, ginseng extract, peony root extract, mulberry leaf extract, tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring uniformly;
(6) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.
Example 2
A skin care essence containing cell extract comprises the following components in parts by weight: 10 parts of purple sweet potato cell extracting solution, 2 parts of ginseng extract, 0.5 part of peony root extract, 0.25 part of mulberry leaf extract, 0.08 part of tremella polysaccharide, 0.4 part of nicotinamide, 0.2 part of tocopherol, 0.5 part of trehalose, 0.5 part of betaine, 2 parts of glycerol, 3 parts of dipropylene glycol, 0.4 part of xanthan gum, 0.25 part of triethanolamine, 0.08 part of EDTA disodium and 65 parts of water.
The preparation method of the skin care essence containing the cell extracting solution comprises the following steps:
(1) taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 1.5, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the enzymolysis temperature of 50 ℃ for 120min, wherein the addition amount of the alpha-amylase is 0.01% of the weight of the raw material of the purple sweet potato pulp, and the addition amount of the glucoamylase is 0.01% of the weight of the raw material of the purple sweet potato pulp, so as to prepare a saccharification liquid of the purple sweet potatoes; inoculating yeast from the inclined plane into wort culture medium with mass ratio of soluble solid of 6.0%, and culturing at 28-30 deg.C and 150r/min for 24 hr; inoculating 6% yeast seed culture solution at volume ratio, culturing at 28 deg.C for 3-4 days with pH of 3.0, and filtering after fermentation.
(2) Centrifuging the purple sweet potato cell culture solution at 3000r/min for 20min, removing the precipitate, and freezing and pulverizing the supernatant at ultralow temperature with liquid nitrogen to obtain purple sweet potato cell powder;
(3) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing at-80 ℃ for five times, centrifuging at 3-5 ℃ of 8000r/min for 20min, taking supernatant as purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;
(4) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, a thickening agent and a chelating agent into an emulsifying pot, stirring, heating to 85 ℃, stirring, and sterilizing at constant temperature for 25 min;
(5) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 42 ℃, then adding accurately weighed purple sweet potato cell extract, ginseng extract, peony root extract, mulberry leaf extract, tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring uniformly;
(6) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.
Example 3
A skin care essence containing cell extract comprises the following components in parts by weight: 15 parts of purple sweet potato cell extracting solution, 4 parts of ginseng extract, 1 part of peony root extract, 0.5 part of mulberry leaf extract, 0.2 part of tremella polysaccharide, 0.6 part of nicotinamide, 0.4 part of tocopherol, 1.0 part of trehalose, 1.5 parts of betaine, 5 parts of glycerol, 5 parts of dipropylene glycol, 0.5 part of thickening agent, 0.4 part of triethanolamine, 0.12 part of EDTA disodium and 80 parts of water.
The preparation method of the skin care essence containing the cell extracting solution comprises the following steps:
(1) taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 2.5, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the enzymolysis temperature of 70 ℃ for 60min, wherein the addition amount of the alpha-amylase is 0.09% of the weight of the raw material of the purple sweet potato pulp, and the addition amount of the glucoamylase is 0.05% of the weight of the raw material of the purple sweet potato pulp, so as to prepare a purple sweet potato saccharification liquid; inoculating yeast from the inclined plane into wort culture medium with mass ratio of soluble solid of 6.0%, and culturing at 28-30 deg.C and 150r/min for 24 hr; inoculating 12% yeast seed culture solution according to volume ratio, culturing at 30 deg.C for 4 days with pH value of 3.5, and filtering after fermentation.
(2) Centrifuging the purple sweet potato cell culture solution at 5000r/min for 20min, removing precipitate, and freezing and pulverizing the supernatant at ultralow temperature with liquid nitrogen to obtain purple sweet potato cell powder;
(3) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing for five times at-80 ℃, centrifuging for 10min at 3-5 ℃ of 11000r/min, taking supernatant fluid which is purple sweet potato cell extracting solution, and storing for later use at 3-5 ℃;
(4) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, thickening agent and chelating agent into an emulsifying pot, stirring, heating to 90 ℃, stirring, and sterilizing at constant temperature for 20-25 min;
(5) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 48 ℃, then adding accurately weighed purple sweet potato cell extract, ginseng extract, peony root extract, mulberry leaf extract, tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring uniformly;
(6) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.
Comparative example 1
This comparative example is the same as example 1 except that no purple sweet potato cell extract was added.
Comparative example 2
This comparative example is the same as example 1 except that ginseng extract, peony root extract, and mulberry leaf extract were not added.
Comparative example 3
This comparative example is the same as example 1 except that the purple sweet potato cell extract was not added, and the ginseng extract, peony root extract and mulberry leaf extract were not added.
To further understand the present invention, the inventors organized 90 volunteers aged between 30-55 years to be tested, all volunteers being healthy women. The 90 volunteers were divided into 6 groups of 15 individuals, the first group tested the product of comparative example 1, the second group tested the product of comparative example 2, the third group tested the product of comparative example 3, the fourth group tested the product of experimental example 1, the fifth group tested the product of experimental example 2 and the sixth group tested the product of experimental example 3.
(1) Skin elasticity detection
The subject applied the serum product twice a day, morning and evening, using the method: cleaning hand skin, wiping, smearing essence on right hand skin, and comparing corresponding symmetric region of left hand with no sample. During the experiment, the subject did not use any other cosmetic at the experimental site. The elasticity of the applied skin area was measured 24 hours after each application for 8 consecutive weeks, and before each measurement, the subject was left in a constant temperature, constant humidity (20 ℃, 60% RH) laboratory for 15 minutes, and the skin elasticity was measured with a skin elasticity measuring instrument (Cutometer dual MPA580) three times per spot, and the average measured value was compared with the control group.
TABLE 1 rate of change of skin elasticity values
As can be seen from table 1, after comparing the products prepared in comparative examples 1 to 3 and examples 1 to 3, respectively, the skin elasticity increasing rate of the samples of comparative example 3 (i.e., no extract of purple sweet potato cells is added, no ginseng extract, peony root extract, and mulberry leaf extract is added) is not significant, the skin elasticity increasing rate of the samples of comparative example 2 (i.e., no extract of ginseng, peony root extract, and mulberry leaf is added) and the skin elasticity increasing rate of the samples of comparative example 1 (i.e., no extract of purple sweet potato cells is added) is significantly increased, and the skin elasticity increasing rate is significantly increased after the extracts of purple sweet potato cells, ginseng, peony root, and mulberry leaf are added, which indicates that the synergistic effects of the active ingredients of purple sweet potato cell extract, ginseng extract, peony root extract, and mulberry leaf extract are generated. With the increase of the using time, the skin care essence containing the cell extracting solution has a remarkable improvement effect on the skin elasticity.
(2) Moisture content detection
Subjects applied the serum product twice a day, morning and evening. The use mode is as follows: after cleansing the facial skin, 1 tablet per day, morning and evening, was used continuously for 2 months, during the experiment, the subject did not use any other cosmetic on the experimental site, and was tried for two months (60 days).
Before the skin moisture tester is used for the first time, the skin moisture content of the forehead, the left face, the right face and the chin is respectively tested by using a FISCIEL SK-III type handheld digital display skin moisture tester, the arithmetic mean value is taken, the skin moisture content of the forehead, the left face, the right face and the chin at the same position is tested by using the skin moisture tester 1 hour after each use, and the arithmetic mean value is taken; the test was carried out for 60 days, on days 30 and 60, respectively, and the test method was the same as that of day 1, the subjects were kept in a constant temperature, constant humidity (20 ℃, 60% RH) laboratory for 15 minutes before the measurement, and the results were arithmetically averaged for each group of 15 volunteers, and the test results are shown in table 2:
TABLE 2 rate of change of skin moisture content values
| Comparative example 1 | Comparative example 2 | Comparative example 3 | Example 1 | Example 2 | Example 3 | |
| Day 0 | 22.9％ | 22.5％ | 23.2％ | 24.3％ | 23.4％ | 22.8％ |
| Day 1 | 25.6％ | 24.5％ | 24.1％ | 30.6％ | 29.2％ | 28.7％ |
| Day 30 | 28.4％ | 26.3％ | 26.1％ | 34.4％ | 33.0％ | 32.6％ |
| Day 60 | 34.3％ | 31.8％ | 31.4％ | 44.1％ | 41.6％ | 42.2％ |
| Rate of change | 11.4％ | 9.3％ | 8.2％ | 19.8％ | 18.2％ | 19.4％ |
As can be seen from table 2, after the comparison of the products prepared in comparative examples 1 to 3 and examples 1 to 3, respectively, the moisturizing effect of the sample in comparative example 3 (i.e., no purple sweet potato cell extract is added, nor is the ginseng extract, the peony root extract, or the mulberry leaf extract added) is weak, the moisturizing effect of the sample in comparative example 2 (i.e., no ginseng extract, the peony root extract, or the mulberry leaf extract added) and the moisturizing effect of the sample in comparative example 1 (i.e., no purple sweet potato cell extract is added) are slightly improved, and the moisturizing effect is remarkably enhanced after the purple sweet potato cell extract, the ginseng extract, the peony root extract, or the mulberry leaf extract is added, which indicates that the active ingredients purple sweet potato cell extract, the ginseng extract, the peony root extract, and the mulberry leaf extract produce synergistic effects. With the increase of the using time, the skin care essence containing the cell extracting solution prepared in the examples 1 to 3 can obviously improve the moisture content of the facial skin.
(3) Detection of antioxidant Activity
The determination principle is as follows: the salicylic acid capture method is adopted. OH generated by Fenton reaction can oxidize salicylic acid to generate 2, 3-dihydroxy benzoic acid with special absorption to 510nm light, and the OH clearance rate is determined by measuring a product obtained by capturing OH by salicylic acid.
The determination method comprises the following steps: sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube4Solution 3mL, 1mmol/L H202Shaking the solution 3mL, adding salicylic acid solution 3mL 6mmol/L, shaking, heating in water bath at 37 deg.C for 15min, and measuring absorbance A0. Then adding 1mL of the samples of comparative examples 1-3 and examples 1-3 (1 mL of distilled water is used for replacing the solution to be detected for the blank), shaking up, continuing to heat in water bath for 15min, taking out and detecting the absorbance Ax(Axx). The clearance rate of hydroxyl radical of the liquid to be tested is calculated according to the following formula: clearance rate ═ axx-Ax)/A0×100％。
The results of the hydroxyl radical scavenging ability of the samples of comparative examples 1 to 3 and examples 1 to 3 are shown in table 3.
TABLE 3 hydroxyl radical scavenging Rate
| Comparative example 1 | Comparative example 2 | Comparative example 3 | Example 1 | Example 2 | Example 3 | |
| Clearance rate | 58.3％ | 63.9％ | 41.7％ | 76.1％ | 72.5％ | 73.9％ |
As can be seen from Table 3, the clearance of hydroxyl radicals is not obvious in the samples of comparative example 3 (i.e. no purple sweet potato cell extract is added, nor is the ginseng extract, the peony root extract and the mulberry leaf extract added), the clearance of hydroxyl radicals is slightly improved in the samples of comparative example 2 (i.e. no ginseng extract, the peony root extract and the mulberry leaf extract are added) and the clearance of hydroxyl radicals is greatly improved in the samples of comparative example 1 (i.e. no purple sweet potato cell extract is added), and the clearance of hydroxyl radicals is greatly improved after the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract are added, so that the synergistic effect of the active ingredients of the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract.
(4) Skin allergy and irritation
All women tested had no allergic reaction and no skin irritation during the test period, the product was mild and non-irritating, and was highly safe.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (5)
1. A skin care essence containing cell extract is characterized in that: the composition is prepared from the following components in parts by weight: 10-15 parts of purple sweet potato cell extract, 2-4 parts of ginseng extract, 0.5-1 part of peony root extract, 0.25-0.5 part of mulberry leaf extract, 0.08-0.2 part of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickener, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water;
the preparation method of the skin care essence containing the cell extracting solution comprises the following steps:
(1) centrifuging the purple sweet potato cell culture solution, removing the precipitate, freezing and crushing the supernatant at ultralow temperature by using liquid nitrogen to obtain purple sweet potato cell powder;
(2) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing for five times at-80 ℃, centrifuging at 3-5 ℃, taking supernate, namely purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;
(3) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, thickening agent and chelating agent into an emulsifying pot, stirring, heating to 85-90 deg.C, stirring, and sterilizing at constant temperature for 20-25 min;
(4) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 42-48 deg.C, adding accurately weighed purple sweet potato cell extractive solution, Ginseng radix extract, peony root extract, folium Mori extract, Tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring;
(5) starting circulating cooling water, continuously stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging;
the extraction method of the purple sweet potato cell culture solution comprises the following steps: taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to a weight ratio of 1: 1.5-2.5, and then carrying out enzymolysis by adopting alpha-amylase and saccharifying enzyme at 50-70 ℃ for 60-120min, wherein the addition amount of the alpha-amylase is 0.01-0.09% of the weight of the purple sweet potato pulp raw material, and the addition amount of the saccharifying enzyme is 0.01-0.05% of the weight of the purple sweet potato pulp raw material, so as to prepare a purple sweet potato saccharifying liquid; inoculating 6-12% yeast seed culture solution at volume ratio, culturing at 25-30 deg.C for 3-4 days with pH of 3.0-3.5, and filtering after fermentation;
in the step (1), the centrifugation conditions are as follows: the rotating speed is 3000-;
in the step (2), the centrifugation conditions are as follows: the rotating speed is 8000-.
2. The skin care essence containing cell extract according to claim 1, wherein: the skin care essence containing the cell extracting solution comprises the following components in parts by weight: 12 parts of purple sweet potato cell extracting solution, 3 parts of ginseng extract, 0.8 part of peony root extract, 0.4 part of mulberry leaf extract, 0.15 part of tremella polysaccharide, 0.5 part of nicotinamide, 0.3 part of tocopherol, 0.8 part of trehalose, 1.15 parts of betaine, 4 parts of glycerol, 4 parts of dipropylene glycol, 0.45 part of thickening agent, 0.3 part of triethanolamine, 0.1 part of chelating agent and 78 parts of water.
3. The skin care essence containing cell extract according to claim 1, wherein: the thickening agent is one or more of carbomer and xanthan gum.
4. The skin care essence containing cell extract according to claim 1, wherein: the chelating agent is disodium EDTA.
5. The skin care essence containing cell extract according to claim 1, wherein: the water is pure water with the conductivity less than or equal to 2 mu S/cm.
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