CN112618404A

CN112618404A - Application of sandalwood polypeptide in preparation of skin care product and composition thereof

Info

Publication number: CN112618404A
Application number: CN202011401624.6A
Authority: CN
Inventors: 李欢; 孙中霞
Original assignee: Tianjin Yunxi Biotechnology Co ltd
Current assignee: Tianjin Yunxi Biotechnology Co ltd
Priority date: 2020-12-02
Filing date: 2020-12-02
Publication date: 2021-04-09

Abstract

The invention provides application of sandalwood polypeptide in preparation of skin care products and a composition thereof, wherein the composition comprises plant extracts, hydroxypropyl tetrahydropyrane triol and deionized water, the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extracts is 1 (2-19), and the plant extracts comprise sandalwood polypeptide, oriental cherry leaf extracts, citrus extracts, egg fruit extracts and tremella polysaccharide. The wrinkle-removing raw materials are reasonable in matching, and are not available, and the prepared finished product has good wrinkle-removing, moisturizing and skin-tendering effects, can effectively remove free radicals, prevent oxidative cells, repair damaged cells, and timely supplement collagen and active substances; the skin care product can keep skin vitality, recover skin luster, can permanently remove wrinkles, can effectively improve the problems of dry, tight and fine lines of the skin, can not cause skin allergy, and has good whitening and moisturizing effects on the skin.

Description

Application of sandalwood polypeptide in preparation of skin care product and composition thereof

Technical Field

The invention relates to application of sandalwood polypeptide in preparation of skin care products and a composition thereof, and belongs to the technical field of cosmetics.

Background

The skin aging is a natural development rule, and factors influencing the skin aging mainly comprise endogenous factors and exogenous factors, wherein the endogenous factors comprise a genetic theory, a metabolite damage theory, a neuro-immune-endocrine dyscrasia theory and the like; extrinsic factors include photoaging, environmental pollution, and the like. The skin aging is characterized notably by the appearance of wrinkles, skin laxity, rough skin, loss of elasticity, pigmentation, etc. Wrinkles on the face are formed by aging of the soft tissues of the face and the movement of the muscles of the face.

CN106726923B discloses a cosmetic additive, which contains one or more of mulberry bark extract, pomegranate flower extract, chamomile extract, cherry leaf essence, oldenlandia diffusa extract and lychee shell extract, wherein each component of the cosmetic additive is plant extract, and the cosmetic additive is safe and has small irritation; can inhibit the formation of melanin at different stages, thereby having synergistic effect on the inhibition of melanin, slowing down the deposition of melanin and improving the whitening effect.

CN110897898A discloses an anti-wrinkle moisturizing eye mask, which comprises ginseng extract, retinol, resveratrol, jellyfish extract, citrus extract, arbutin, papain, polypeptide, trehalose, green tea extract, pearl powder, mint extract, cubilose extract, propolis and other components, wherein the eye mask prepared from the components has good permeability and easy absorption, and has the functions of anti-wrinkle and moisturizing; the anti-wrinkle moisturizing eye mask disclosed by the invention adopts pure natural traditional Chinese medicinal materials, does not contain heavy metals, and has no toxic or side effect.

CN103006460B discloses a skin care composition, which comprises tremella polysaccharide, cell growth factors and edible preservatives, wherein the cell growth factors and the tremella polysaccharide are synergistically prepared into the skin care composition, the cell growth factors and the tremella polysaccharide complement each other in function and complement each other, and the effect of the cooperation of the cell growth factors and the tremella polysaccharide is far greater than the effect of the cooperation of the cell growth factors and the tremella polysaccharide. The invention not only can be used for daily skin care, such as removing skin wrinkles and spots, increasing skin elasticity and luster, but also can be used for the maintenance of sensitive skin, and can be used for wound recovery after wound healing and minimally invasive cosmetic surgery, scar removal and skin beautification.

However, the above products have a fundamental problem that the products cannot simultaneously satisfy the effects of wrinkle removal, moisture retention and whitening, and some products have a plurality of components and high cost, so that the price of the products is improved and the products are not suitable for the public; and some products only aim at local face to remove wrinkles, and have small application range.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a sandalwood polypeptide-containing composition which not only has the effects of removing wrinkles, moisturizing and whitening, but also has the moisturizing effect of more than 8 hours.

In order to achieve the purpose, the invention is realized by the following technical scheme: application of sandalwood polypeptide in preparing skin care products.

Preferably, the skin care product is a wrinkle-removing skin care product.

Preferably, the wrinkle-removing skin care product is prepared from a composition containing sandalwood polypeptide.

A skin care composition comprises a plant extract, wherein the plant extract comprises a sandalwood polypeptide.

Preferably, the mass ratio of the sandalwood polypeptide to the plant extract is 1 (5-20).

Preferably, the mass ratio of the sandalwood polypeptide to the plant extract is 1 (5-10).

Preferably, the mass ratio of the sandalwood polypeptide to the plant extract is 1: 5.

Preferably, the skin care composition also contains hydroxypropyl tetrahydropyrane triol, and the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is 1 (2-19).

Preferably, the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is 1 (2-11).

Preferably, the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is 1: 5.

Preferably, the plant extract further comprises one or more of cherry leaf extract, orange extract, egg fruit extract and tremella polysaccharide.

Preferably, the skin care composition further comprises amino acid, and the amino acid accounts for 5% -20% of the total mass of the composition.

Preferably, the amino acid is one or more of tyrosine, tryptophan, valine, threonine, serine, arginine, glutamine, proline, asparagine, methionine, leucine, lysine, isoleucine, histidine, phenylalanine, glutamic acid, aspartic acid, cysteine and alanine.

Preferably, the skin care composition is used in creams, essences, masks, emulsions, creams, gels.

Preferably, the skin care composition is used for anti-wrinkle cream, anti-wrinkle essence, anti-wrinkle mask and medical dressing.

Preferably, the skin care composition is used for skin external skin care products and medical products.

A skin care composition is prepared by mixing the plant extract, hydroxypropyl tetrahydropyrane triol, amino acid and deionized water in proportion.

The hydroxypropyl tetrahydropyrane triol, also called vitronectin, which is related to the present invention is a glycoprotein mixture made of xylose. It is known to stimulate the production of aminodextran and also to prevent the loss of water from the skin. Hydroxypropyl tetrahydropyrane triol enters the skin and promotes the production and construction of what are known as protein polysaccharides in the extracellular matrix (e.g., gelatinous protein networks and sugars that act as scaffolds and intercellular shock absorbers). These molecules absorb water, making the matrix gel-like, increasing the firmness of the cells and skin. It may also serve as a channel for the passage of repair molecules, such as proteins for cell growth.

According to the invention, sandalwood is a traditional Chinese medicinal material with a beautifying effect, can extract sandalwood polypeptide which directly acts on human skin, can nourish skin, can replenish water for skin, can accelerate skin metabolism, can accelerate skin cell renewal, can repair damaged skin cells, can prevent skin inflammation, can fade spots and acne marks, and can enable skin of people to become better and better. The application increases sandalwood polypeptide component, can increase capillary permeability, improves microcirculation, promotes epidermal cell metabolism, and old and new skin cell is replaced with higher speed, makes the skin plump, is rich in elasticity. And the sandalwood polypeptide adopted in the application is prepared by the preparation method disclosed in CN 111073941A.

In the invention, the oriental cherry leaf extract mainly has the effects of an antioxidant, whitening and freckle removal and milk beautifying in cosmetics and skin care products, has a risk coefficient of 1, is safe, can be used safely, generally has no influence on pregnant women, and has no pox-causing property. Has broad-spectrum scavenging effect on various free radicals, and can be used as antioxidant for preventing aging; has good promoting effect on hair growth, and can be used for preventing alopecia and nourishing hair; has effects in inhibiting tyrosinase activity, and can be used for whitening skin; meanwhile, the composition has certain anti-inflammatory effect.

In the invention, after the glucuronic acid in the molecular structure of the tremella polysaccharide is decomposed, the released glucuronic acid monomer is a precursor substance for synthesizing hyaluronic acid, and can promote the synthesis of hyaluronic acid in vivo and improve the skin state.

In the present invention, amino acids are the most basic substances that constitute biological proteins and are involved in their life activities, and are the basic units that constitute protein molecules in the body, and are closely related to the life activities of the organism. It has special physiological function in antibody and is one of the essential nutrients for organism. The amino acid component can activate cells, improve metabolism and blood circulation, revive the skin, distinguish the skin from dullness, has high-efficiency moisturizing function, and can strengthen and replenish the moisture of the skin according to seasons to keep the skin moist. Compared with the common hyaluronic acid, the hyaluronic acid has better transparent effect, can smooth dry lines, can strengthen the resisting function of skin to protect the skin, avoids the damage caused by the change of external environment, and prevents and improves the dry skin.

It is well known that moisture is a key factor for skin health and is the basis for other care, and even a good product can only play a role in the condition of balanced and healthy skin moisture. The skin has increased moisture content and can be seen as smoother and glossy. The skin moisture index is reduced to a certain degree, the skin is in a dry state, the skin looks rough and loose, the time is long, and wrinkles appear.

A large number of experiments show that when the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is 1 (2-19), the skin moisture and moisturizing effect is optimal, and the wrinkle removing effect can be further better achieved.

There are three major areas of free radical damage to the human body: firstly, cell membranes are damaged; secondly, the activity of the serum anti-protease is lost; and thirdly, the damaged gene causes the occurrence and accumulation of cell variation. The skin of people can generate a plurality of free radicals in the environments of sunshine, air pollution and the like, the problems of dark yellow, fine lines and uneven skin color can be caused when the free radicals of the skin are excessive, and the oxidation resistance is needed to be realized when the damage caused by the free radicals is reduced.

A large number of experiments prove that the compatibility of the sandalwood polypeptide, the cherry leaf extract, the orange extract, the egg fruit extract and the tremella polysaccharide can better eliminate free radicals and achieve the antioxidation effect.

The invention has the beneficial effects that:

(1) the wrinkle-removing raw materials are reasonable in matching, and are not available, and the prepared finished product has good wrinkle-removing, moisturizing and skin-tendering effects, can effectively remove free radicals, prevent oxidative cells, repair damaged cells, and timely supplement collagen and active substances; the skin care product can keep skin vitality, recover skin luster, can permanently remove wrinkles, can effectively improve the problems of dry, tight and fine lines of the skin, can not cause skin allergy, and has good whitening and moisturizing effects on the skin.

(2) The plant extract is added, so that the moisturizing duration of the composition is prolonged, and the effects of removing wrinkles, moisturizing and whitening can be simultaneously met.

(3) According to the invention, through the compatibility of the sandalwood polypeptide, the cherry leaf extract, the orange extract, the passion fruit extract and the tremella polysaccharide, the composition can better eliminate free radicals, and the antioxidation effect is achieved.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

Example 1 compositions containing Santalum album polypeptide

The amino acid is threonine, serine, arginine and glutamine, wherein the mass ratio of threonine to serine to arginine to glutamine is 1:1:1: 1.

Mixing lignum Santali albi polypeptide, folium Pruni Pseudocerasi extract, hydroxypropyl tetrahydropyrane triol, amino acids and deionized water at a certain ratio.

Example 2 compositions containing Santalum album polypeptide

The amino acid is isoleucine, histidine, phenylalanine and glutamic acid, wherein the mass ratio of the isoleucine to the histidine to the phenylalanine to the glutamic acid is 1:1:1: 1.

Mixing lignum Santali albi polypeptide, citrus extract, hydroxypropyl tetrahydropyrane triol, amino acid and deionized water at a certain proportion.

Example 3 compositions containing Santalum album polypeptide

The amino acid is tyrosine, tryptophan, valine and proline, wherein the mass ratio of the tyrosine to the tryptophan to the valine to the proline is 1:1:1: 1.

Mixing sandalwood polypeptide, eggfruit extract, hydroxypropyl tetrahydropyrane triol, amino acid and deionized water according to a proportion.

Example 4 compositions containing Santalum album polypeptide

The amino acid is asparagine, methionine, leucine and lysine, wherein the mass ratio of asparagine to methionine to leucine to lysine is 1:1:1: 1.

The gel is prepared by mixing sandalwood polypeptide, tremella polysaccharide, hydroxypropyl tetrahydropyran triol, amino acid and deionized water according to a proportion.

Example 5 compositions containing Santalum album polypeptide

The amino acid is phenylalanine, glutamic acid, aspartic acid, cysteine and alanine, wherein the mass ratio of the phenylalanine, the glutamic acid, the aspartic acid, the cysteine and the alanine is 1:1:1: 1.

Mixing the sandalwood polypeptide, the tremella polysaccharide, the citrus extract, the hydroxypropyl tetrahydropyrane triol, the amino acid and the deionized water according to a proportion.

Example 6 compositions containing Santalum album polypeptide

The amino acid is serine, arginine and glutamine, wherein the mass ratio of the serine to the arginine to the glutamine is 1:2: 1.

Mixing lignum Santali albi polypeptide, Tremella polysaccharide, Mandarin orange extract, fructus Plumeriae Acutifoliae extract, hydroxypropyl tetrahydropyrane triol, amino acids and deionized water at a certain proportion.

Test example 1 Effect of the ratio of hydroxypropyl tetrahydropyrane triol to plant extract on moisturizing Effect

The composition is prepared according to the proportion in the table 1, 60 persons are randomly selected, the age is 30-40 years old, the proportion of male and female is 1:1, the compositions are divided into 6 groups, the testing period is 12h, and the skin moisture content is used as the testing index.

Before application, the subjects washed their faces with the same cleanser, and selected 5 × 5cm areas as experimental areas at symmetrical positions on the left and right sides of the face of the subjects, and applied 0.5g of the composition prepared in the control group to the left face and 0.5g of the composition prepared in the test example to the right face, and the subjects performed daily detection and nighttime detection in a room with the same temperature and humidity, and performed skin humidity detection at regular time intervals within 12 hours after application, as shown in table 2.

TABLE 1 composition of the compositions

The plant extracts in table 1 are sandalwood polypeptide, cherry mosaic extract, orange extract, passion fruit extract and tremella polysaccharide, and the mass ratio of the sandalwood polypeptide, the cherry mosaic extract, the orange extract, the passion fruit extract and the tremella polysaccharide is 1:1:1:1: 1.

TABLE 2 skin moisture test results of the test subjects

Referring to table 2, the moisture retention time of the left face to which the control composition was applied was short and the moisture content of the skin was low within 1 to 12 hours after application, whereas the moisture retention time of the right face to which the composition of the comparative example was applied was as long as 5 hours or more, and when 8 hours later, the moisture content of the skin suddenly decreased.

When the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is between 1 (2-19), the skin moisture-retaining effect is the best, so the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is selected to be 1 (2-19).

Test example 2 scavenging action of content of Santalum album polypeptide on free radical

(1) Activity test for scavenging hydroxyl radical (. OH)

The test method comprises the following steps: by means of H₂O₂With Fe²⁺The mixing reaction generates OH, salicylic acid is added into the system to capture OH and generate a colored substance which has maximum absorption at the wavelength of 510 nm. The reaction system contains 1mL of 8.8mmol/LH₂O₂、1mL 9mmol/L FeSO₄1mL of a 9mmol/L salicylic acid-ethanol solution and 1mL of the compositions of comparative examples 7-12 at a concentration of 10%, distilled water being the reference. Ascorbic acid, TBHQ, was used as a control, and each concentration was repeated 5 times, and averaged.

The mass ratio of hydroxypropyl tetrahydropyrane triol to plant extract in comparative examples 7-12 was 1:5, i.e.: 10g of hydroxypropyl tetrahydropyrane triol, 50g of plant extract, 20g of amino acid and 10g of deionized water, wherein the amino acid is specifically 3g of serine, 4g of arginine and 3g of glutamine, the mass ratio of the sandalwood polypeptide to the plant extract is shown in table 3, and the test result is shown in table 4.

TABLE 3 Mass ratio of Santalum album polypeptide to plant extract

TABLE 4 detection of scavenging free radicals

As can be seen from table 4, the composition of the present invention has stronger ability to scavenge free radicals than ascorbic acid and TBHQ, although the sandalwood polypeptide is not added in comparative example 7, but comparative example 7 also has stronger ability to scavenge free radicals, but when the sandalwood polypeptide is added in the composition, the ability to scavenge free radicals is significantly enhanced, but as the content of the sandalwood polypeptide increases, the ability to scavenge free radicals gradually decreases, and when the ratio exceeds 1:20, the ability to scavenge free radicals suddenly decreases, which may be because the content of the sandalwood polypeptide increases and the content of other plant extracts correspondingly decreases, and the synergistic effect between the plant extracts cannot be better achieved, thereby resulting in the decrease of the ability to scavenge free radicals.

(2) Measurement of DPPH scavenging ability

The test method comprises the following steps: the characteristic absorption peak of DPPH solution is used, and the reduction of absorbance after adding antioxidant or whey oligopeptide solution indicates the scavenging capacity of the DPPH solution on free radicals. DPPH was prepared to 0.2mmol/L in methanol, 2mL of this solution was reacted with 2mL of the composition of comparative examples 7 to 12 having a concentration of 10% in a dark room for 30min, absorbance was measured at a wavelength of 517nm, and each concentration was repeated 5 times to take an average value. The control group comprises ascorbic acid and TBHQ, and the test results are shown in Table 5.

TABLE 5 measurement of DPPH scavenging ability

Group of	Control rate/%)
		Comparative example 7	79.15
Comparative example 8	98.88
		Comparative example 9	99.01
Comparative example 10	99.16
		Comparative example 11	98.08
Comparative example 12	80.11
		Ascorbic acid	30.56
TBHQ	99.21

As can be seen from table 5, the composition of the present invention has a stronger ability to scavenge DPPH than ascorbic acid, and is substantially the same as TBHQ, when sandalwood polypeptide is added to the composition, the ability to scavenge free radicals is significantly enhanced, and when the ratio exceeds 1:20, the ability to scavenge free radicals is suddenly reduced, which may be caused by the fact that the content of sandalwood polypeptide is increased, the content of other plant extracts is correspondingly reduced, and the synergistic effect between the plant extracts cannot be better achieved, resulting in the reduction of the ability to scavenge free radicals.

Test example 3 wrinkle-removing Effect

(1) Test samples: examples 1 to 6, control group in test example 1, and composition of comparative example 7.

(2) Test method

240 wrinkled volunteers are selected, the male-female ratio is 1:1, the age is 30-45 years old, freckles are on the face, the cosmetics are not allergic, other hormone medicines or immunosuppressants are not used, and the groups are randomly divided into 8 groups, and each group comprises 30 people.

The test sample is smeared on the wrinkles, 1mL each time, once in the morning and at night, and is continuously used for 30 days. After 30 days, the use feeling and the skin texture condition are specifically analyzed.

(3) Evaluation criteria

A: the effect is good, fine lines can be reduced by naked eyes, the skin moisture detected by a skin moisture tester is more than 45%, and freckles become light;

b: generally, wrinkles become slightly shallow but not obvious, the skin moisture measured by a skin moisture tester is more than 30%, and individual freckles become pale;

c: the skin moisture content detected by a skin moisture tester is less than 30%, and freckles are unchanged.

The details of the evaluation are shown in Table 6.

TABLE 6 evaluation of wrinkle-removing Effect after 30 days of use

As is clear from table 6, the compositions of examples 1 to 6 of the present invention can suppress the lightening of fine wrinkles, dry wrinkles, etc., and make the skin firm and smooth, and can improve the skin moisture by using the compositions for a long period of time.

While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The application of the sandalwood polypeptide in preparing the skin care product is characterized in that the skin care product is a wrinkle-removing skin care product.

2. The use of claim 1, wherein the wrinkle-removing skin care product is prepared from a composition comprising a sandalwood polypeptide.

3. A skin care composition comprising a plant extract, wherein said plant extract comprises a sandalwood polypeptide.

4. The skin care composition according to claim 3, wherein the mass ratio of the sandalwood polypeptide to the plant extract is 1 (5-20), preferably 1 (5-10), more preferably 1: 5.

5. The skin care composition according to claim 3 or 4, wherein the skin care composition further comprises hydroxypropyl tetrahydropyrane triol, and the mass ratio of the hydroxypropyl tetrahydropyrane triol to the plant extract is 1 (2-19), preferably 1 (2-11), more preferably 1: 5.

6. the skin care composition according to claim 5, wherein the plant extract further comprises one or more of cherry leaf extract, orange extract, passion fruit extract, and tremella polysaccharide.

7. The skin care composition of claim 6, further comprising an amino acid, wherein the amino acid comprises 5% to 20% of the total composition by weight.

8. The skin care composition according to claim 7, wherein the amino acid is one or more of tyrosine, tryptophan, valine, threonine, serine, arginine, glutamine, proline, asparagine, methionine, leucine, lysine, isoleucine, histidine, phenylalanine, glutamic acid, aspartic acid, cysteine, and alanine.

9. The skin care composition according to claim 8, wherein the skin care composition is used in a cream, serum, emulsion, cream, gel, dressing, medical product.

10. A method of making the skin care composition of claim 9 wherein the plant extract, hydroxypropyl tetrahydropyran triol, amino acids and deionized water are mixed in proportions.