CN109330954B

CN109330954B - Whitening skin brightening lotion, preparation method thereof and tyrosinase inhibitor

Info

Publication number: CN109330954B
Application number: CN201811601743.9A
Authority: CN
Inventors: 杨登亮; 刘德海; 戴嘉彬; 江兰兰; 李华真; 林盛杰; 张伟杰; 张楚标
Original assignee: GUANGZHOU BAIYUN LIANJIA FINE CHEMICAL PLANT; Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Current assignee: Guangdong Danz Group Co Ltd; Guangzhou Keneng Cosmetic Research Co Ltd
Priority date: 2018-12-26
Filing date: 2018-12-26
Publication date: 2022-03-11
Anticipated expiration: 2038-12-26
Also published as: CN109330954A

Abstract

The invention provides whitening toner, a preparation method thereof and a tyrosinase inhibitor. The whitening and skin-brightening lotion comprises a tyrosinase inhibitor; the tyrosinase inhibitor comprises a kudzu root extract and a revived grass extract, wherein the addition amount of the kudzu root extract is 0.5-75% and the addition amount of the revived grass extract is 25-99.5% based on the total mass of the tyrosinase inhibitor. The whitening skin-brightening lotion disclosed by the invention is mild in formula, and can effectively inhibit the activity of tyrosinase, so that the whitening effect is achieved. Further, the tyrosinase inhibitor of the present invention is excellent in the inhibitory effect on the activity of tyrosinase.

Description

Whitening skin brightening lotion, preparation method thereof and tyrosinase inhibitor

Technical Field

The invention relates to a whitening toner, a preparation method thereof and a tyrosinase inhibitor, belonging to the field of cosmetics.

Background

Facial pigmentation is a skin problem of great concern. Chloasma, freckles and melanin deposits affect the appearance, thereby causing troubles to people. Whitening additives can reduce the deposition of black or reduce the skin color, and Chinese women are increasingly pursuing fair skin, so that the demand of whitening and brightening lotion is continuously increased.

Most of the existing whitening additives in the prior art are organic compounds, and the substances have irritation to skin, large side effect, increased harm to human bodies and reduced safety factor; in addition, a single plant extract is also adopted as a whitening additive, but the plant extract has a single whitening component, a single action mechanism and an unobvious whitening effect.

Patent application document CN101904809A discloses a whitening composition containing cortex mori extract, chamomile extract, rhodiola rosea extract, grape seed extract, licorice extract, morus alba extract, arbutin, kojic acid dipalmitate, niacinamide, tetrahydrocurcumin, a humectant and a preservative, which can inhibit the activity of tyrosinase to some extent, thereby blocking melanin transport and inhibiting melanin formation. However, it contains organic compounds kojic acid dipalmitate enzyme and nicotinamide, and is harmful to human body.

Disclosure of Invention

Problems to be solved by the invention

In view of the problems of poor effect, poor whitening effect and the like of the Chinese herbal medicine whitening and freckle removing preparation in the prior art, the invention firstly provides whitening and skin brightening lotion. The whitening and skin-brightening lotion has excellent inhibition effect on tyrosinase activity, can play a whitening role and has no side effect on human bodies.

Further, the present invention provides a tyrosinase inhibitor having an excellent inhibitory effect on tyrosinase activity.

Further, the invention also provides a preparation method of the whitening and skin-brightening lotion, which is simple to operate and easy to obtain raw materials.

Means for solving the problems

The invention provides a whitening and skin-brightening lotion, which comprises a tyrosinase inhibitor; the tyrosinase inhibitor comprises a kudzu root extract and a revived grass extract, wherein the addition amount of the kudzu root extract is 0.5-75% and the addition amount of the revived grass extract is 25-99.5% based on the total mass of the tyrosinase inhibitor.

The whitening and skin-brightening lotion provided by the invention is characterized in that the mass ratio of the kudzu root extract to the revival grass extract is 1: 0.4-90, preferably 1: 1-80, more preferably 1:2 to 70, and more preferably 1: 2.5 to 60, and more preferably 1: 3 to 55, and more preferably 1: 3.5 to 50.

The whitening and skin-brightening lotion provided by the invention is characterized in that the tyrosinase inhibitor is added in an amount of 0.01-12% by mass based on the total mass of the whitening and skin-brightening lotion.

The whitening and skin-brightening lotion further comprises a humectant, a thickener, a solubilizer, a skin conditioner, a soothing agent and a preservative; wherein the addition amount of the humectant is 0.01-20% by the total mass of the whitening and skin-brightening lotion; the addition amount of the thickening agent is 0.02-0.8%; the addition amount of the solubilizer is 0.01-0.5%; the addition amount of the skin conditioner is 0.01-5%; the addition amount of the allergy relieving agent is 0.01-5%; the addition amount of the preservative is 0.01-1.5%.

The whitening and skin-brightening lotion comprises one or a combination of more than two of dipropylene glycol, propylene glycol, butylene glycol, panthenol, glycerol, polyethylene glycol-32, glyceryl polyether-26 and sodium hyaluronate; and/or

The thickening agent comprises one or more than two of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, hydroxyethyl cellulose, xanthan gum, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, carbomer and ammonium acryloyldimethyl taurate/VP copolymer.

The whitening and skin-brightening water comprises one or more of polysorbate-20, PEG-40 hydrogenated castor oil and PPG-26-butanol polyether-26; and/or

The antiseptic comprises one or more of phenoxyethanol, methyl hydroxybenzoate, propyl hydroxybenzoate, benzoic acid and its salt.

The whitening and skin-brightening lotion comprises one or more of a giant kelp extract, vitamin E, avenin, ceramide 2, a fucus extract, a chlorella fermentation product, hydrolyzed collagen, hydrolyzed wheat protein, a green algae extract, a brown algae extract, allantoin, a lactobacillus/soybean fermentation product extract, a mistletoe ginkgo leaf extract, a cogongrass rhizome extract, a cactus extract and trehalose; and/or

The soothing agent comprises Hamamelis virginiana water, flos Matricariae Chamomillae extract, herba Portulacae extract, herba Centellae extract, rhizoma Zingiberis recens extract, bisabolol, and dipotassium glycyrrhizinate.

The invention also provides a tyrosinase inhibitor, which comprises a kudzu root extract and a revived grass extract, wherein the addition amount of the kudzu root extract is 0.5-75% and the addition amount of the revived grass extract is 25-99.5% based on the total mass of the tyrosinase inhibitor.

The tyrosinase inhibitor provided by the invention is characterized in that the mass ratio of the kudzu root extract to the revival grass extract is 1: 0.4-90, preferably 1: 1-80, more preferably 1:2 to 70, and more preferably 1: 2.5 to 60, and more preferably 1: 3 to 55, and more preferably 1: 3.5 to 50.

The invention also provides a preparation method of the whitening and skin-brightening lotion, which comprises the step of mixing the components of the whitening and skin-brightening lotion.

ADVANTAGEOUS EFFECTS OF INVENTION

The whitening skin-brightening lotion disclosed by the invention is mild in formula, and can effectively inhibit the activity of tyrosinase, so that the whitening effect is achieved.

Further, the tyrosinase inhibitor of the present invention is excellent in the inhibitory effect on the activity of tyrosinase.

Furthermore, the preparation method of the whitening and skin-brightening lotion is simple to operate, easily obtains raw materials, and can be produced in batch.

Drawings

FIG. 1 is a graph showing the relationship between logarithmic mass concentration and tyrosinase inhibition rate of the extract of Pueraria lobata of comparative examples 1 to 5 of the present invention;

FIG. 2 is a graph showing log mass concentration versus tyrosinase inhibition for extracts of reconstituted grass according to comparative examples 6-9 of the present invention;

FIG. 3 is a graph showing the log mass concentration of tyrosinase inhibitors versus tyrosinase inhibition in accordance with examples 1-7 of the present invention.

FIG. 4 is a graph showing the relationship between the content of Pueraria lobata extract and the interaction coefficient in tyrosinase inhibitors of examples 1-7 of the present invention.

FIG. 5 is a graph showing a comparison of the melanin change rates of application examples 1 to 8 and application comparative examples 1 to 2 of the present invention.

Detailed Description

Various exemplary embodiments, features and aspects of the invention will be described in detail below. The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.

Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In other instances, methods, means, devices and steps which are well known to those skilled in the art have not been described in detail so as not to obscure the invention.

First embodiment

A first embodiment of the invention provides a tyrosinase inhibitor, comprising a tyrosinase inhibitor; the tyrosinase inhibitor comprises radix Puerariae extract and revived herba Hedyotis Diffusae extract. The tyrosinase inhibitor can well inhibit the activity of tyrosinase, and has an excellent whitening effect when being used in cosmetics, particularly in whitening and brightening lotion.

Radix Puerariae is dried root of Pueraria lobata (wild Ohwi) or Pueraria thomsonii Benth (Pueraria thomsonii Benth) of Leguminosae, which is also known as radix Puerariae. China has 11 kinds of Pueraria plants in total, and the Pueraria starch is mainly cultivated artificially, and the rest is mainly wild. The Chinese pharmacopoeia (1990 edition) contains two Pueraria plants, i.e., Pueraria and Pueraria.

The kudzu root mainly comprises the following components: starch, cellulose, protein and total flavonoids, and further contains small amount of fat, pectin, tannin, alkaloid, etc. The starch of kudzu root contains 11 amino acids and 11 mineral elements, wherein the microelements necessary for human body include Fe, Cu, Zn, Mn, Si, etc. The pueraria flavonid contains isoflavone component, which is the main active component of pueraria.

The kudzu root extract is generally used for anti-aging cosmetics, can smooth the face, has the efficacy of removing acne and wrinkles, and has the mechanism related to that kudzu root isoflavones have the functions of resisting oxidation and enhancing the immunity of organisms. The radix Puerariae isoflavone has effects of scavenging oxygen free radicals and resisting lipid peroxidation, and can significantly inhibit erythrocyte hemolysis caused by oxidative damage, and inhibit active oxygen (-OH, O) to microsome^2-The product can improve the activity strength of SOD in vivo and regulate the balance of oxygen free radicals and free radical scavenger in vivo, so that radix Puerariae has good anti-aging effect.

Under the condition of extreme drought, branches and leaves of the revival grass can be tightly contracted into balls, the last precious drop of water in the body is firmly locked, and the revival grass can survive for decades even if the height of the revival grass loses 98 percent of water. Once the rare rainy season is met, the grass revival body contains rich glycosyl trehalose which can play a strong water locking and moisturizing function, then the water containing life activity is smoothly and freely guided into each cell in the body, the water which is 460 times of the weight of the cell is locked, the branches and leaves can be stretched again, and the living green life is recovered.

The resurrection grass extract is applied to cosmetics, can adjust the immune function of skin, enhance the disease resistance of the skin, eliminate inflammation, prevent allergy, clean the skin and simultaneously relieve the toxic and side effects of the cosmetics and other external factors on the skin.

In addition, the revival grass extract can also enhance the activity and metabolism of cells, activate and regenerate skin cells, enhance the oxidation resistance of the skin cells, reduce the redness of the skin, resist the erythema, accelerate the healing of wounds and the tissue repair, slow down the aging process of the fiber cells in the dermis and improve the collagen production in the aging cells by 36 percent.

The kudzu root extract and the resurrection grass extract have a synergistic effect by using the combination of the kudzu root extract and the resurrection grass extract, and can further play a role in inhibiting the activity of tyrosinase. In the invention, the addition amount of the kudzu root extract is 0.5-75% and the addition amount of the revival grass extract is 25-99.5% of the total mass of the tyrosinase inhibitor. In the present invention, the pueraria extract and the revived grass extract can be combined in any ratio.

Preferably, the mass ratio of the kudzu root extract to the revival grass extract is 1: 0.4-90, preferably 1: 1-80, more preferably 1:2 to 70, and more preferably 1: 2.5 to 60, and more preferably 1: 3 to 55, and more preferably 1: 3.5 to 50. When the mass ratio of the pueraria extract to the revived grass extract is within the above range, a synergistic effect can be further achieved, and the effect of inhibiting tyrosinase activity is excellent.

Second embodiment

A second embodiment of the present invention provides a whitening and skin-lightening lotion comprising the tyrosinase inhibitor of the first embodiment. According to the invention, the tyrosinase inhibitor with a proper content is added, so that the activity of tyrosinase can be inhibited, and the whitening and skin-brightening lotion has an excellent whitening effect.

Wherein, the adding amount of the tyrosinase inhibitor is 0.01-12%, preferably 0.1-10% of the total mass of the whitening and skin-brightening water, such as: 0.5%, 1%, 2%, 3%, 5%, 6%, 7%, 8%, etc. When the amount of tyrosinase inhibitor is 0.01-12%, the melanin content in skin is reduced after the skin whitening and brightening lotion is used. When the addition amount of the tyrosinase inhibitor is less than 0.01%, the reduction amount of melanin content is small, and the whitening effect cannot be achieved; when the adding amount of the tyrosinase inhibitor is more than 12%, the content of the tyrosinase inhibitor is too high, the cost is too high, and the corresponding whitening effect is not obviously improved.

The whitening and skin-brightening lotion can also comprise a humectant, a thickening agent, a solubilizer, a skin conditioner, a soothing agent and a preservative. The formula of the whitening and skin-brightening lotion is mild, so that the effect of the tyrosinase inhibitor can be fully exerted. Specifically, the method comprises the following steps:

the addition amount of the humectant is 0.01-20% of the total mass of the whitening and skin-brightening lotion. When the addition amount of the humectant is 0.01-20%, the humectant can play a role in moisturizing and hydrating. In order to further exert the efficacy of the moisturizer, the amount of the moisturizer of the present invention added may be preferably 1 to 19%, 4 to 18%, 6 to 17%, 8 to 16%, 10 to 15%, or the like. When the content of the humectant is less than 0.01%, the moisturizing effect is not obvious; when the content of the humectant is more than 20%, the whitening and skin-brightening lotion has a sticky feeling.

In the invention, the humectant comprises one or more of dipropylene glycol, propylene glycol, butylene glycol, panthenol, glycerin, polyethylene glycol-32, glyceryl polyether-26 and sodium hyaluronate. The whitening and skin-brightening lotion provided by the invention has excellent moisturizing performance by using a combination of a plurality of humectants.

The addition amount of the thickening agent is 0.02-0.8% of the total mass of the whitening and skin-brightening lotion. When the addition amount of the thickener is between 0.02 and 0.8 percent, the low-viscosity feeling and excellent use feeling are achieved, the dispersibility is good, and the absorption is fast. When the addition amount of the thickener is less than 0.02%, the whitening and skin-brightening lotion is thin in texture and does not have any sticky feeling; when the thickener is added in an amount of more than 0.8%, the whitening and skin lightening lotion is too thick and heavy, which may increase the burden on the skin.

In the invention, the thickening agent comprises one or more than two of acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, hydroxyethyl cellulose, xanthan gum, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, carbomer and acryloyldimethyl taurate/VP copolymer.

The addition amount of the solubilizer is 0.01-0.5% based on the total mass of the whitening and skin-brightening lotion. The solubilizer is used in the skin whitening and brightening water, so that the skin feel of the skin whitening and brightening water is smooth. Preferably, the solubilizer is added in an amount of 0.05-0.4%, 0.08-0.3%, etc. In the present invention, the solubilizer includes one or more of polysorbate-20, PEG-40 hydrogenated castor oil, and PPG-26-buteth-26.

In order to further improve the efficacy of the whitening and skin-brightening lotion, the whitening and skin-brightening lotion further comprises a skin conditioner. The skin conditioner is added, so that the effects of moisturizing and moisturizing can be further achieved, and the generation of wrinkles can be reduced. In addition, the skin conditioner can also properly reduce the adhesive force of stratum corneum cells, accelerate the renewal of epidermal cells and enhance the repair capacity of skin.

The addition amount of the skin conditioning agent is 0.01-5% of the total mass of the whitening and skin-brightening water, and can be 0.5%, 1%, 2%, 3%, 4% and the like. When the addition amount of the skin conditioner is less than 0.01%, the content is too low to achieve the corresponding effect.

In the present invention, the skin conditioner includes one or a combination of two or more of a macroalgae extract, vitamin E, avenin, ceramide 2, a fucus extract, a chlorella fermentation product, hydrolyzed collagen, hydrolyzed wheat protein, a green algae extract, a brown algae extract, allantoin, a lactobacillus/soybean fermentation product extract, a ginkgo mistletoe leaf extract, a cogongrass rhizome extract, a cactus extract, and trehalose.

The kelp extract is derived from kelp, and the kelp can increase the content of natural plant protein by the mass propagation of kelp cells. The giant kelp extract has the effects of supplementing cell nutrition and effectively preserving moisture.

The ceramide 2 can be used for improving the sebum membrane and inhibiting the secretion of active sebaceous glands, so that the skin is balanced in water and oil, the self-protection function of the skin is enhanced, and the skin care cream is suitable for the young skin which is greasy and has requirements. The composition has good effects in moisturizing and repairing skin, is an important skin-activating component in horny layer, and can enhance skin barrier and rebuild cells.

Among them, allantoin can reduce the adhesion of stratum corneum cells, accelerate epidermal cell renewal, enhance skin repair ability, and has high safety.

Wherein the avenin is a fragment of avenin and a component of avenin. Oat protein has unique function, and the amino acid species balance of oat protein is far superior to other grains. After the oat protein is decomposed, small molecular peptides with high quality can be generated, and the small molecular peptides are easy to be absorbed by skin. The oat peptide has the effects of resisting oxidation, increasing skin activity, delaying skin aging, moisturizing, reducing wrinkles, resisting allergy and the like.

Wherein the hydrolyzed wheat protein contains gliadin (gliadin) and glutenin (glutenin) as amino acids, and cystine (cystine). The hydrolyzed wheat protein has effects of keeping moisture, resisting oxidation, softening and refining skin, and improving wrinkle.

In addition, the whitening and skin-brightening lotion can also be added with a soothing agent. By adding the allergy relieving agent, the skin can be calmed, so that the skin has certain allergy relieving effect on the injury red swelling of the facial skin, and the skin can be helped to resist inflammation, relieve and promote cell repair.

The amount of the soothing agent added is 0.01-5% by total mass of the whitening and skin-brightening lotion, and can be 0.5%, 1%, 2%, 3% and the like, for example. When the addition amount of the allergy relieving agent is less than 0.01 percent, the allergy relieving effect is not obvious. When the addition amount of the allergy relieving agent is more than 5%, the further allergy relieving effect cannot be achieved, and the cost is too high.

The hamamelis virginiana flower water in the soothing agent belongs to high-concentration plant original dew, has light herbal fragrance, and has the effects of controlling oil, conditioning, astringing, tightening pores, removing stasis and relieving swelling. Can be used for caring skin and soothing skin.

The whitening and skin-brightening lotion can be properly added with a pH regulator, an antioxidant and a chelating agent. Generally, the pH regulator is added in an amount of 0-1% based on the total mass of the whitening and skin-brightening lotion; the addition amount of the antioxidant is 0-2%; the addition amount of the chelating agent is 0-1%. The pH regulator comprises one or more of aminomethyl propanol, arginine, citric acid, sodium citrate, and sodium hydroxide. The antioxidant can be one or more of vitamin E, tocopherol acetate, butylated hydroxytoluene, lycopene, etc. The chelating agent may be EDTA-2Na and/or EDTA-4Na, etc.

The addition amount of the preservative in the whitening and skin-brightening water is 0.01-1.5%. The preservative can comprise one or the combination of more than two of phenoxyethanol, methyl hydroxybenzoate, propyl hydroxybenzoate, benzoic acid and salts thereof. In addition, the whitening and brightening toner also contains an aromatic. The addition amount of the aromatic is 0.005-0.5% of the total mass of the whitening and skin-brightening lotion. The aromatic may be a perfume, etc.

Third embodiment

The second embodiment of the invention also provides a preparation method of the whitening and skin-brightening lotion, which comprises the step of mixing the components of the whitening and skin-brightening lotion.

Specifically, the preparation method of the whitening and skin-brightening lotion provided by the invention can comprise the following steps:

1. adding water, humectant, thickener, part of skin conditioner, optional chelating agent, optional pH regulator, aromatic and part of antiseptic into a stirring pot, stirring, and heating to 80-85 deg.C;

2. cooling to 40-50 deg.C, adding tyrosinase inhibitor, solubilizer, rest skin conditioner, soothing agent, optional antioxidant and rest antiseptic, and stirring;

3. cooling to 35-40 deg.C, discharging after qualified inspection, and standing for 12-48 hr;

4. and (5) after the inspection is qualified, subpackaging, packaging, inspecting again, and warehousing the finished product.

Examples

Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.

Comparative examples 1 to 5

Taking radix Puerariae extract as tyrosinase inhibitor. The puerariae radix extract was dissolved in 5 sets of phosphate buffers with different volumes and pH 6.8 to obtain 5 sets of test solutions with different concentrations. Wherein, the logarithmic mass concentration of the kudzu root extract is shown in the following table 2.

Comparative examples 6 to 9

Taking the extract of the resurrection grass as the tyrosinase inhibitor. The revival grass extract was dissolved in 4 sets of phosphate buffer solutions having a pH of 6.8 at volumes corresponding to comparative examples 1-4 to obtain 4 sets of test solutions having concentrations corresponding to comparative examples 1-4. Wherein, the logarithmic mass concentration of the revival grass extract is shown in the following table 2.

Examples 1 to 7

Taking radix Puerariae extract and herba Potentillae Fulgentis extract as tyrosinase inhibitor. The tyrosinase inhibitor is obtained by mixing the kudzu root extract and the revived grass extract according to the mass ratio of 2:1 (example 1), 1:1 (example 2), 1:2 (example 3), 15:85 (example 4), 5:95 (example 5), 2:98 (example 6) and 7:68 (example 7). The tyrosinase inhibitors of examples 1-7 were dissolved in 5 volumes of phosphate buffer solutions having a pH of 6.8 corresponding to those of comparative examples 1-5, respectively, and each of examples 1-7 allowed 5 sets of test solutions having a concentration corresponding to those of comparative examples 1-5 to be obtained. Wherein, in the tyrosinase inhibitor, the contents (mass%) of the kudzu root extract and the revived grass extract are shown in the following table 1, and the logarithmic mass concentration of the tyrosinase inhibitor is shown in the following table 3.

TABLE 1

In vitro testing

Tyrosinase activity is measured as the tyrosinase activity that catalyzes the production of dopaquinone from L-dopa. The method specifically comprises the following steps:

taking 1 test tube, adding 0.4mL (1.0mg/mL) of substrate L-dopa solution, adding 2.4mL of phosphate buffer solution with pH of 6.8, carrying out water bath at 30 ℃ for 10min, and then adding 0.2mL (250U/mL) of tyrosinase to obtain reaction solution 1.

After taking 1 tube, 0.2mL (250U/mL) of tyrosinase was added, and 2.8mL of a phosphate buffer solution having a pH of 6.8 was added to the tube, thereby obtaining a reaction solution 2.

0.4mL (1.0mg/mL) of the substrate L-dopa solution was added to each of 44 tubes, and then 2.4mL of the test solutions of comparative examples 1 to 9 and examples 1 to 7 was added, and after incubation in a water bath at 30 ℃ for 10 minutes, the amount of tyrosinase was then added in an amount of 0.2mL (250U/mL), to obtain a reaction solution 3.

2.4mL of the test solutions of comparative examples 1 to 9 and examples 1 to 7 were added to 44 tubes, respectively, and 0.4mL of a phosphate buffer solution having a pH of 6.8 was added, followed by incubation in a 30 ℃ water bath for 10 minutes, and then an amount of tyrosinase of 0.2mL (250U/mL) was added to obtain a reaction solution 4.

The enzymatic reaction converts L-dopa to the red product dopaquinone, with maximum absorption at 475 nm. Read the absorbance A at 475nm 1min from the start of the mixing₄₇₅The rate of the enzymatic reaction is defined as A per minute₄₇₅Is indicated by an increased value of (a). The absorbance A at 475nm of the above reaction solution 1min from the start of the reaction was measured, and the inhibition rate of tyrosinase activity was calculated according to the following formula. Wherein the mass concentrations of the tyrosinase inhibitors in the table are the final mass concentrations of the tyrosinase inhibitors in the test system.

Inhibition rate ═ 1- (A)₃-A₄)/(A₁-A₂)]×100％

In the formula: a. the₁Absorbance when substrate alone was included without adding tyrosinase inhibitor;

A₂absorbance without adding tyrosinase inhibitor and without adding substrate;

A₃is the absorbance of the mixture containing the tyrosinase inhibitor and the substrate;

A₄is the absorbance when the tyrosinase inhibitor is contained and no substrate is added.

The inhibition rates of the pueraria lobata extract (comparative examples 1-5) and the revival grass extract (comparative examples 6-9) on the tyrosinase activity were calculated respectively. And calculating the corresponding mass concentration (IC) when the inhibition rate of radix Puerariae extract is 50% by combining with the relation graph of logarithmic mass concentration-tyrosinase inhibition rate_50A) Mass concentration (IC) corresponding to 50% inhibition ratio of the revival grass extract_50B) The results are shown in Table 2.

TABLE 2

The tyrosinase inhibitors of examples 1-7 were then tested for their inhibition of tyrosinase activity. And calculating the mass concentration (IC) of radix Puerariae extract when the combined action of radix Puerariae extract and revived grass extract generates equivalent inhibition rate (50%) by combining the logarithmic mass concentration-tyrosinase inhibition rate relationship diagram_50a) The mass concentration (IC) of the revival grass extract when the compound action of the kudzu root extract and the revival grass extract generates equivalent inhibition rate (50 percent)_50b) The results are shown in Table 3.

The effect of the combined action of the pueraria extract and the revived grass extract can be evaluated by the interaction coefficient γ, and the specific results are shown in table 3.

γ＝IC_50a/IC_50A+IC_50b/IC_50B

Wherein, IC_50AThe inhibition rate of the kudzu root extract is 50 percentMass concentration of (d);

IC_50Brepresents the mass concentration corresponding to the 50% inhibition rate of the revival grass extract;

IC_50athe mass concentration of the kudzu root extract is shown when the compound action of the kudzu root extract and the resurrection grass extract generates equivalent inhibition rate (50%);

IC_50bthe mass concentration of the resurrection grass extract is shown when the compound action of the kudzuvine root extract and the resurrection grass extract generates an equivalent inhibition rate (50%).

Wherein γ ═ 1, indicates that the pueraria extract and revived grass extract exhibit a simple additive effect; gamma is less than 1, the radix puerariae extract and the resurrection grass extract show a synergistic effect, and the smaller the gamma value is, the stronger the synergistic effect is; gamma is more than 1, the kudzu root extract and the resurrection grass extract show antagonistic effect, and the larger the gamma value is, the larger the antagonistic effect is.

TABLE 3

As can be seen from table 3, the tyrosinase inhibitor of the present invention has an interaction coefficient of less than 1, and the interaction coefficient value thereof may be 0.65 or less, and may be 0.5 or less, so that the puerariae radix extract and the revived grass extract may exhibit excellent synergistic effects.

Application examples 1 to 8

The whitening skin-brightening lotion is prepared according to the content (mass percentage) of each component in the formula of the whitening skin-brightening lotions of the application examples 1 to 8 in the following tables 4 to 5 and according to the following production process steps. The production process comprises the following steps:

1. adding the raw materials of the phase A, the phase B and the phase C into a stirring pot, stirring and heating to 82-85 ℃;

2. cooling to 42 deg.C, adding phase D, phase E and phase F, and stirring;

3. cooling to 37 ℃, discharging after the inspection is qualified, and standing for 24 hours;

Note: the phase A, the phase B, the phase C, the phase D, the phase E and the phase F in the process are respectively,

phase A: water;

phase B: butanediol, glycerol, propylene glycol, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate/VP copolymer, sodium hyaluronate, panthenol, dipropylene glycol;

and C phase: methylparaben, allantoin, glyceryl polyether-26, polyethylene glycol-32;

phase D: PEG-40 hydrogenated castor oil, essence;

phase E: phenoxyethanol, ceramide 2, trehalose;

and (3) phase F: radix Puerariae extract, revived grass extract, Macrocystis extract, Hamamelis Virginiana flower water, hydrolyzed wheat protein, and oat peptide.

The kudzu root extract and the resurrection grass extract used in the formula are tyrosinase inhibitors;

butanediol, glycerol, propylene glycol, sodium hyaluronate, panthenol, dipropylene glycol, glyceryl polyether-26, and polyethylene glycol-32 as humectant;

hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer and acryloyl dimethyl ammonium taurate/VP copolymer are thickening agents;

the skin conditioner comprises Macrocystis extract, ceramide 2, hydrolyzed wheat protein, oat peptide, allantoin, and trehalose;

PEG-40 hydrogenated castor oil is a solubilizer; hamamelis virginiana flower water is a soothing sensitizer;

phenoxyethanol and methyl hydroxybenzoate as antiseptic; the essence is an aromatic.

In the invention, the kudzu root extract is prepared by the following steps: quzhou City exhibition-macro biotechnology Co., Ltd;

resurrection lily rhizome extract, the manufacturer: quzhou City exhibition-macro Biotechnology Ltd.

TABLE 4

Application of comparative examples 1 to 2

Skin lightening water was prepared according to the contents (mass percentages) of the components in the skin lightening water formulations of comparative application examples 1 to 2 in the following table 5 in the same manner as in application examples 1 to 8.

TABLE 5

Whitening efficacy test

Method for testing skin melanin change rate: the content of melanin in the skin is determined by measuring the reflection of light of a specific wavelength on the skin of a human body. The emitter of the probe emits light with three wavelengths of 568nm, 660nm and 880nm to the skin surface, the receiver measures the light reflected by the skin and measures the amount of light absorbed by the skin, so as to obtain the change rate of skin melanin.

The test of the skin melanin change rate is carried out by using a pigment detector of CK company in Germany, wherein a test probe MX 18 of the skin melanin consists of a light source emitter and a light source receiver, and a spring is additionally arranged to keep the pressure on the skin constant during the detection. The measuring range of the probe is 0-999, and the higher the measuring value is, the higher the content of melanin in the skin can be indicated.

The number of the subjects is 33, the test period is 4 weeks, the whitening skin-lightening lotion of application examples 1-8 and the skin-lightening lotion of application comparative examples 1-2 are selected in the test, the skin-lightening lotion is applied to different areas of the inner side of the forearm every morning and evening, the reflection amount of the skin of the tested area before the test (Day1) and after the skin is used for 28 days (Day28) after the light with specific wavelength is respectively measured to determine the content of the melanin in the skin, and then the change rate of the melanin is characterized, and the result of the specific melanin change rate is shown in fig. 5.

As can be seen from fig. 5, the change rate of melanin is large, that is, the melanin content is reduced, in the application examples 1 to 8 of the present application, and thus, the skin can be effectively whitened by using the pueraria lobata extract and the revival grass extract as tyrosinase inhibitors.

In addition, in application examples 6 to 8 of the present application, since the content of the pueraria lobata extract was high, the melanin content was less decreased.

In the application comparative examples 1-2, when the pueraria extract or the revival grass extract is used as the tyrosinase inhibitor, the change rate of the melanin content is small, the melanin content is reduced a little, and the whitening effect is poor.

The above examples of the present invention are merely examples for clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A whitening and skin-brightening lotion is characterized by comprising a tyrosinase inhibitor, a humectant, a thickener, a solubilizer, a skin conditioner, a soothing agent and a preservative; the adding amount of the tyrosinase inhibitor is 0.01-12% of the total mass of the whitening and skin-brightening lotion; the addition amount of the humectant is 0.01-20%; the addition amount of the thickening agent is 0.02-0.8%; the addition amount of the solubilizer is 0.01-0.5%; the addition amount of the skin conditioner is 0.01-5%; the addition amount of the allergy relieving agent is 0.01-5%; the addition amount of the preservative is 0.01-1.5%;

the tyrosinase inhibitor is a kudzu root extract and a revived grass extract, the addition amount of the kudzu root extract is 0.5-75% and the addition amount of the revived grass extract is 25-99.5% based on the total mass of the tyrosinase inhibitor, and the mass ratio of the kudzu root extract to the revived grass extract is 1: 3.5 to 50;

the pueraria lobata extract is obtained from thoroughfare vessel exhibitory biotechnology limited, and the revival grass extract is obtained from thoroughfare vessel exhibitory biotechnology limited.

2. The whitening toner according to claim 1, wherein the humectant comprises one or a combination of two or more of dipropylene glycol, propylene glycol, butylene glycol, panthenol, glycerin, polyethylene glycol-32, glyceryl polyether-26, and sodium hyaluronate; and/or

3. The whitening and skin-lightening lotion according to claim 1 or 2, wherein the solubilizer comprises one or more of polysorbate-20, PEG-40 hydrogenated castor oil and PPG-26-buthaneth-26; and/or

The preservative comprises one or the combination of more than two of phenoxyethanol, methyl hydroxybenzoate, propyl hydroxybenzoate, benzoic acid or benzoate.

4. The whitening and skin-lightening lotion according to claim 1 or 2, wherein the skin conditioner comprises one or a combination of two or more of a macroalgae extract, vitamin E, oat peptide, ceramide 2, a fucus extract, a chlorella fermentation product, hydrolyzed collagen, hydrolyzed wheat protein, a green algae extract, a brown algae extract, allantoin, a lactobacillus/soybean fermentation product extract, a mistletoe ginkgo leaf extract, a cogongrass rhizome extract, a cactus extract, and trehalose; and/or

5. The preparation method of the whitening and skin-brightening lotion as claimed in any one of claims 1 to 4, characterized by comprising the step of mixing the components of the whitening and skin-brightening lotion.