KR101796286B1 - Cosmetic Composition Comprising fermented Selaginella Tamariscina - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented liquid of Selaginella tamariscina as an active ingredient, and more particularly, to a cosmetic composition comprising, as an active ingredient, a fermented liquid of Selaginella tamariscina, which is fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof. The present invention also relates to a cosmetic composition further comprising dried mature seeds of Canavalia Gladiata. The cosmetic composition comprising the fermented liquid of Selaginella tamariscina or fermented liquids of Selaginella tamariscina and dried mature seeds of Canavalia Gladiata, according to the present invention, is excellent in formulation stability and has an excellent skin whitening effect without skin irritation.

Description

[0002] Cosmetic Composition Comprising fermented Selaginella tamariscina as an active ingredient,

More particularly, the present invention relates to a cosmetic composition comprising a rocky hand fermentation broth as an active ingredient, and more particularly, to a cosmetic composition comprising a rocky hand fermentation broth fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof To a cosmetic composition. The present invention further relates to a cosmetic composition further comprising a dairy fermentation broth. The cosmetic composition comprising the rock-hand fermentation broth or the rock-and-spoon fermentation liquid according to the present invention is excellent in formulation stability and has excellent skin whitening effect without skin irritation. It also has additional skin moisturizing, gloss enhancement and elasticity enhancement.

Skin is a tissue covering the outside of the body. It acts as a barrier to the external environment, prevents external leakage of water and electrolytes, controls body temperature, performs sensory functions such as tactility, pressure, and temperature stimulation, performs immune function, and produces vitamin D Function.

In recent years, there has been an increasing interest in skin cosmetics, and there is an increasing demand for cosmetic compositions having functionalities such as skin whitening, moisturizing, gloss enhancement, or elasticity enhancement. Describing skin whitening among these functionalities, for example, ascorbic acid and its derivatives or tyrosinase inhibitors are mainly used as whitening agents.

Tyrosinase is a copper-containing metal enzyme that catalyzes the oxidation of tyrosine and phenols by oxygen. It is present in most plant and animal tissues and is known to be involved in the formation of melanin pigmentation in animal tissues and the darkening of cut parts of mushrooms, potatoes, apples and the like. As the tyrosinase inhibitor, hydroquinone derivatives such as arbutin, kojic acid dipalmitate, and kojic acid derivatives such as kojic acid tripeptide are widely used, and cyclo acetic acid derivatives, chalcone derivatives, N-benzyl benzamide, stilbene derivatives , Procyanidin derivatives, butyne derivatives, alkylfuranone, centauridine, chlorophorine, and Magnolysin have been studied.

However, ascorbic acid and its derivatives are highly stable in human body, but have little whitening effect, and hydroquinone derivatives used as tyrosinase inhibitors are cytotoxic to melanocytes, and are allowed to be used as cosmetic compositions in Europe, Japan, and South Africa And is very susceptible to oxidation, making it difficult to stabilize it in cosmetic composition formulations. Another tyrosinase inhibitor, kojic acid, causes skin sensitization, such as exhibiting contact allergies.

Therefore, it is required to develop a cosmetic composition having safety and excellent functionality without side effects. Recently, in response to this demand, development of a cosmetic composition using natural materials has been actively performed.

On the other hand, 'Selaginella Tamariscina' is the 'Evergreen herb with the winged bamboo plant of the vascular plant', which grows on the rock. The undergrowth extends between the ground and the gypsophila and grows straight to 15 ~ 40 cm. It divides into 3-4 quartz like ovate or long ovate leaves, and the bottom part is petiole. Leaves are scaly and arranged in 4 rows, similar in shape to the base but in the upper part in two forms and with a sawtooth on the edge. The sporophyllous spore [spore 囊 穗] runs on one end of the small branch and is squared. The sporophyll is a triangular egg-shaped, 5 ~ 25 mm in length and 1 mm in diameter, and distributed in Korea, Japan and China. The above-mentioned rock-like hand is a natural plant preparation which has been used safely for a long time by folk remedies. The main ingredients include flavones, phenolic components, amino acids, polysaccharides such as trehalose, and small amounts of tannins.

Dodu is dried bean seeds of Canadian Bean (Canavalia Gladiata). The taste is sweet and the quality is warm. It acts on the stomach (胃 经) and the colon (肠肠 经). It warms the middle chest and shows the 肾气 (chestnut) by lowering the chest. It exhibits antitumor activity in pharmacological experiments. Huh Hanseong hiccups, vomiting, abdominal bloating, Shinhei low back pain (肾 虛 腰痛), sputum comes out to cough.

On the other hand, as a prior art related to the present invention, Korean Patent No. 10-0924920 discloses a composition for preventing or treating acne, which contains a rock extract of a hand.

The above-mentioned rock extract of the prior art is characterized by being an extract derived from water, an organic solvent, or a mixture thereof, and has an effect of inhibiting the growth of acne bacterium and thus alleviating acne.

However, the composition of the prior art has a problem that turbidity is high due to a large amount of suspended matters, a turbidity is high, a precipitate is generated in the production of the product, layer separation occurs, In addition, the composition of the prior art inhibits the growth of acne bacterium and does not have an effect on improvement of the skin condition.

Accordingly, the present inventor has developed a cosmetic composition which is superior in skin whitening, moisturizing, gloss enhancement, or elasticity-enhancing effect without any skin irritation by using a rocking hand and a doodle.

1. Korean Patent No. 10-0924920

The present invention is to provide a cosmetic composition having natural skin whitening effect without skin irritation using a natural product. The present invention also provides a cosmetic composition which has moisturizing, gloss enhancement or elasticity-enhancing effect and improves the skin condition.

In order to solve the above technical problems, the present invention provides a cosmetic composition comprising Selaginella tamariscina fermentation broth as an active ingredient.

According to one aspect of the present invention, the rocky hand fermentation broth is characterized in that a rock or hand extract of a rock is fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof.

According to one aspect of the present invention, the above-mentioned rocky hand fermentation broth is obtained by inoculating Lactobacillus bacteria, Bifidobacteria or a mixture thereof, and then fermenting at 30 to 40 DEG C for 5 to 20 days .

According to one aspect of the present invention, there is provided a cosmetic composition comprising, as an active ingredient, a rocky hand and a fermentation liquid of Canavalia Gladiata.

According to one aspect of the present invention, the edible fermentation broth is characterized in that a rock or hand extract is fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof.

According to one aspect of the present invention, the soybean fermentation broth is characterized by being obtained by inoculating Lactobacillus bacteria, Bifidobacteria or a mixture thereof, and then fermenting at 35 to 40 DEG C for 5 to 20 days .

According to one aspect of the present invention, the cosmetic composition according to the present invention is characterized in that it is for skin whitening, moisturizing, moisturizing, or elasticity enhancement.

According to one aspect of the present invention, a cosmetic composition according to the present invention is used as a cosmetic composition for cosmetics such as a convergent lotion, a soft lotion, a skin lotion, a cream, an essence, a nutrition gel, a lipstick, a foundation, an eye shadow, a pack, a patch, a mast sheet, Rinses, treatments, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

According to one aspect of the present invention, there is provided a method for sterilizing a rock, comprising: sterilizing a rocked hand by heating at 100 to 130 DEG C for at least 15 minutes; Adding Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rocks; And a step of extracting the fermented rock hands. The present invention also provides a method for producing a cosmetic composition.

According to one aspect of the present invention, there is provided a method for sterilizing a rock, comprising the steps of: Fermenting by adding Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rock hands and toes; And extracting the fermented rock hands and the tofu. The present invention also provides a method for producing a cosmetic composition.

The cosmetic composition according to the present invention is excellent in formulation stability and has an excellent skin whitening effect without skin irritation. It also has skin moisturizing, luster enhancement and elasticity enhancing effect and has an effect of improving the skin condition.

The cosmetic composition according to the present invention improves the skin tone and reduces the skin spot by including the rocky hand fermented product or the rocky hand and the dried fermented product which inhibits the synthesis of melanin, and has an excellent skin whitening effect.

The whitening effect of the cosmetic composition according to the present invention has a whitening effect superior to that of the rock extract or the rock extract.

1 is a flow chart showing a manufacturing method of an embodiment according to the present invention.
2 is a photograph before and after applying the embodiment according to the present invention to human skin.

In order to accomplish the above object, the present invention provides a cosmetic composition comprising the fermentation broth of Selaginella tamariscina, further comprising a cosmetic composition comprising an active ingredient, and a fermentation broth thereof, and a method for producing the same.

The present invention is characterized by a cosmetic composition comprising an effective ingredient of a fermentation broth of Selaginella tamariscina.

The Selaginella tamariscina fermentation broth can be fermented with Lactobacillus bacteria, Bifidobacteria, or a mixture thereof, by hand or rock extract.

When preparing the fermentation broth, various portions of the rock hands can be suitably used. For example, the fermentation broth can be used in various ways such as leaves, roots, stem, The rock hands may be those that have not undergone any pretreatment, and may be subjected to pretreatment such as drying, cutting, immersion and the like. Preferably, the washed hand of the rocks is cut into 2-20 mm size, and the hands are immersed in water at room temperature for 1 to 5 hours. In this case, the rock hands may be used in an amount of 3 to 20% by weight, preferably 5 to 15% by weight, based on the weight of water.

In addition, it is possible to ferment an extract of a rocky hand, and appropriately prepared rock hands according to the above method can be subjected to extraction by a hot water extraction method, a solvent extraction method, a compression extraction method, a steam distillation method, a carbon dioxide supercritical extraction method, a steam distillation method, a microwave process extraction method, And the like.

The fermentation broth of Selaginella tamariscina according to the present invention can be produced using lactic acid bacteria, Bacillus subtilis, Mycelia, Yeast, Porphyra, etc., preferably lactic acid bacteria. For example, rock or hand extracts of rocks may be fermented with Lactobacillus bacteria, Bifidobacteria or mixtures thereof.

The rocky hand fermentation broth of the present invention is prepared by inoculating fermentation broth Lactobacillus bacteria, Bifidobacteria or a mixture thereof in an appropriate amount, for example, at a concentration of ¹⁸ CFU / mL, To 20 days, more preferably 5 to 10 days.

In addition, the present invention provides a cosmetic composition comprising as an active ingredient a fermentation broth of Canavalia Gladiata.

The fermentation broth of Selaginella tamariscina can be prepared as described above, and the fermentation broth can also be produced in the same manner. On the other hand, rocky hands and doodulizing fermentation broth can be produced by fermenting the rock hands and the doodu together or by mixing the respective fermentation broths.

Therefore, the fermentation broth of Canavalia Gladiata can be fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof, and Lactobacillus bacteria, Bifidobacteria, Or a mixture thereof, and then fermenting at 35 to 40 DEG C for 5 to 20 days.

On the other hand, the above-mentioned rocky hand fermentation broth, rocky hand, and doodle mixed fermentation broth can be subjected to appropriate extraction or filtration to provide a cosmetic composition. Various extraction methods such as hot water extraction method, solvent extraction method, compression extraction method, steam distillation method, carbon dioxide supercritical extraction method, steam distillation method, microwave process extraction method and phenolation extraction method can be used. can do. The filtration methods include membrane separation, centrifugation, sedimentation, fractional distillation, and the like. Preferably, a micro-network filtration method can be used. In addition, the filtration step can be repeatedly performed. Preferably, the precipitate of the fermentation broth can be removed using a sedimentation method or centrifugation method after filtration using micro-nets. In addition to the steps described above according to the design conditions, further steps such as concentration, extraction, etc. may be performed.

According to one aspect of the present invention, the cosmetic composition according to the present invention may be added with a substance selected arbitrarily in accordance with the purpose of formulation or use of other cosmetic ingredients in addition to the rock-like fermented product, the rocky hand, and the fermented product. For example, purified water, an oil, a surfactant, a moisturizer, a high alcohol, a thickener, a chelating agent, a pigment, a fatty acid, an antioxidant, an antiseptic, a wax, a pH adjusting agent and a perfume may be added.

Examples of the oil include mineral oil, cyclomethicone, squalane, octyldodecyl myristate, olive oil, macadamia nut oil, glyceryl octanoate, castor oil, ethylhexyl isononanoate, dimethicone, cyclopentasiloxane, Sunflower seed oil and the like can be used.

Examples of the surfactant include sorbitan sesquiunorate, polysorbate 60, glyceryl stearate, pro-type glyceryl stearate, sorbitan oleate, sorbitan stearate, diai-cetyl phosphate, sorbitan stearate / Sucrose cocoate, glyceryl stearate / polyethylene glycol-100 stearate, cetearyl-6 olivate, arachidyl alcohol / behenyl alcohol / arachidyl glucoside. Polypropylene glycol-26-butes-26 / polyethylene glycol-40 hydrogenated castor oil and the like can be used.

Examples of the moisturizing agent include glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, diglycerin, betaine, glycereth-26 and methylglucose-20.

As the higher alcohol, cetyl alcohol, stearyl alcohol, octyldodecanol, isostearyl alcohol and the like can be used.

Examples of the thickener include xanthan gum, carbomer, magnesium aluminum silicate, cellulose gum, dextrin palmitate, and the like.

As the chelating agent, disodium dithiophosphate, tetrasodium dithiophosphate and the like may be used.

Examples of the dye include Blue No. 1, Red No. 40, Yellow No. 4, and Yellow No. 5.

Examples of the fatty acid include stearic acid, myristic acid, palmitic acid, isostearic acid, lauric acid, and the like.

Examples of the antioxidant include tocopheryl acetate, butylated hydroxytoluene, and the like.

Examples of the preservative include methylparaben, butylparaben, propylparaben, phenoxyethanol, imidazolidinyl urea, chlorphenesin, and the like.

As the wax, carnauba wax, candelilla wax, beeswax, and wax can be used.

Examples of the pH adjuster include triethanolamine, citric acid, and the like.

As the fragrance, natural fragrance, combination fragrance and the like can be used.

The formulation of the cosmetic composition of the present invention is not particularly limited as long as it is a conventional cosmetic formulation, and examples thereof include cosmetic lotion, soft lotion, skin, lotion, cream, essence, nutrition gel, lipstick, foundation, eye shadow, Packs, patches, mast sheets, soaps, shampoos, rinses, treatments, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

The present invention relates to a method for sterilization by sterilizing a rocky hand by heating at 100 to 130 DEG C for at least 15 minutes; Adding Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rocks; And extracting the fermented rock hands. The present invention also provides a method for producing a cosmetic composition.

According to one aspect of the present invention, there is provided a method for sterilizing a rock, comprising the steps of: Fermenting by adding Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rock hands and toes; And extracting the fermented rock hands and doodles.

The process of sterilization is intended to minimize the undesirable effects of bacteria other than the fermenting bacteria in the fermentation process. The heating can be performed by heating at 100 to 130 ° C for 15 minutes or more, preferably sterilized by heating at 100 to 110 ° C for 15 to 20 minutes. The amount of the fermenting bacteria to be added and the extraction process are as described above.

Hereinafter, the present invention will be described in more detail with reference to Examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.

Example  1. Manufacture of stone-hand fermentation broth 1

The rock hands are washed and dried in the shade (wheat dry). Cut the dried rock hands into 2 ~ 4mm size. 18kg of the cut stone hands 180kg of water, more than 3 hours at 25 ℃ water temperature is called. The rock hands are sterilized by heating at 100 ° C for 15 minutes. After sterilized stone hands are cooled to 35-40 ℃, 100g of lactic acid bacteria is added, fermented in 7-8 days in winter, and 4-5 days in summer, spring and autumn. The resulting fermented product is cooled, put in a compactor, put in a microwave oven after filtration, and precipitated for more than one week. Concentrate the product (to a concentration of about 60-70%).

Example  2. Manufacture of rock broth fermentation broth 2

The rock hands are washed and dried in the shade (wheat dry). Cut the dried rock hands into 2 ~ 4mm size. 18kg of the cut stone hands 180kg of water, more than 3 hours at 25 ℃ water temperature is called. The rock hands are placed in a squeezer, put into a microwave oven after being juiced, filtered and allowed to settle for more than one week. The obtained rock extract is sterilized by heating at 100 ° C for 15 minutes. After sterilized stone hands are cooled to 35-40 ℃, 100g of lactic acid bacteria is added, fermented in 7-8 days in winter, and 4-5 days in summer, spring and autumn. Concentrate the product (to a concentration of about 60-70%).

Example  3. Rock hands and Doodoo  Production of fermentation broth 1

The rock hands are washed and dried in the shade (wheat dry). Cut the dried rock hands into 2 ~ 4mm size. 18kg of the cut stone hands 180kg of water, more than 3 hours at 25 ℃ water temperature is called. The rock hands are sterilized by heating at 100 ° C for 15 minutes and then cooled. 30 degrees at the end of the water at 25 degrees Celsius, 180 kilograms of water, put more than 3 hours. Sterilize the doodle by heating at 100 ° C or more for 15 minutes or more, and cool it. After mixing the above-prepared rock hands and doodu, 100 g of lactic acid bacteria are added, fermented for 7-8 days in winter, and fermented for 4-5 days in summer and spring autumn. The resulting fermented product is cooled, put in a compactor, put in a microwave oven after filtration, and precipitated for more than one week. Concentrate the product (to a concentration of about 60-70%).

Example  4. Rock hands and Doodoo  Preparation of fermentation broth 2

The rock hands are washed and dried in the shade (wheat dry). Cut the dried rock hands into 2 ~ 4mm size. 18kg of the cut stone hands 180kg of water, more than 3 hours at 25 ℃ water temperature is called. 30 degrees at the end of the water at 25 degrees Celsius, 180 kilograms of water, put more than 3 hours. After mixing the above-prepared rock hands and doodle, put them in a press machine, put them in a microwave oven after juicing, and set them for more than one week. The obtained mixed extract of rocks and bean curd are sterilized by heating at 100 ° C for 15 minutes. After the sterilized mixture is cooled to 35-40 ° C, 100g of lactic acid bacteria is added and fermented 7-8 days in winter and 4-5 days in summer and spring fall. Concentrate the product (to a concentration of about 60-70%).

Manufacturing example  1. Rock hands and Doodoo  Cream containing fermentation liquid

Examples of the preparation of the cream including the rocky hands and the fermentation broth are as shown in Table 1 below.

ingredient Content (% by weight) (Example 3) < RTI ID = 0.0 > 60.0 C13-14 isoparaffin (C13-14 Isoparaffin) 8.0 Laureth-7 (Laureth-7) 4.0 Butylene Glycol 4.0 Dimethicone Copolyol 4.0 Cyclomethicone 3.0 Glycerin 3.0 Alcohol 2.0 Trehalose 2.0 Niacinamide 1.0 Dimethyl Sulfone 1.0 Isohexadecane 0.8 Preservative, fragrance handful Purified water Balance Sum 100.0

Manufacturing example  2. Rock hands and Doodoo  Fermentation skin

Examples of the preparation of the skin including the rock-like hands and the fermentation liquid of Doodu are as shown in Table 2 below.

ingredient
Content (% by weight)
Skin 1 Skin 2 Skin 3 (Example 3) < RTI ID = 0.0 > 30.0 50.0 87.0 Dimethyl Sulfone 10.0 7.0 5.0 PEG-40 Hydrogenated Castor Oil (PEG-40 Hydrogenated Castor Oil) 8.0 5.0 3.0 26-Buteth-26 (PPG-26-Buteth-26) 5.0 3.0 1.0 Preservative, fragrance handful handful handful Purified water Balance Balance Balance Sum 100.0 00.0 100.0

Manufacturing example  3. Rock hands and Doodoo  Soap containing fermentation broth

Examples of the production of soaps including the rock hands and the fermentation broth are shown in Table 3 below.

ingredient Content (% by weight) Soap Chip 82.0 (Example 3) < RTI ID = 0.0 > 15.0 Fragrance 1.0 Glycerin 0.5 Tocopherol 0.3 Hyaluronic Acid 0.3 Collagen 0.2 Preservative, pigment handful Sum 100.0

Experimental Example 1. Melanogenesis inhibition rate

Melanocytes are present in the basal layer of the epidermis and produce melanin pigment by ultraviolet rays, oxidative stress, genetic factors, etc., and affect skin tone. Therefore, skin whitening effect of the present invention was confirmed by measuring inhibition rate of melanin pigment formation.

B16F10 cells were cultured and stabilized, and each sample was treated for 48 hours. The cells were then removed by centrifugation at 10,000 to 13,000 rpm for 15 minutes. The resulting pellet was washed with alcohol, and the cells were washed with 1 N NaOH solution supplemented with 10% DMSO The incubation time was 1 hour in a thermostat bath to dissolve the melanin and the absorbance was measured at 405 nm.

Based on the absorbance of the untreated group, the inhibition rate of melanin formation in the experimental group was calculated.

The rocky hand extract and tofu extract used as comparative examples were prepared in the same manner as in the above example except for the fermentation step, and the fermented product obtained by fermenting soybeans only without mixing with the rock hands. As a comparative example, it was also compared with the arbutin-treated group used as a whitening agent.

sample Treatment concentration (mM) Inhibition rate (%) Example 1 (Rock Hand Fermentation Solution) One 43.1 Example 3 (Rock stone + Tongdu fermentation liquid) 1 (0.5 + 0.5) 52.2 Comparative Example 1 (rock extract) One 35.1 Comparative Example 2 (extract of Tohoku) One 27.8 Comparative Example 3 (Tongue fermentation broth) One 33.2 COMPARATIVE EXAMPLE 4 (rock extract + Todo extract) 1 (0.5 + 0.5) 32.8 Comparative Example 5 (Arbutin) One 48.3

The inhibition rate of melanin formation was 27.8%, 32.8%, 34.2%, 35.1%, and 43.1%, respectively, ) And rocky hand + doodle fermentation broth (52.2%). These results indicate that the fermented broth mixed with rocky hand fermented broth and rocky hands has excellent whitening effect.

Especially, the fermented liquid mixed with rocky hands and doodles showed about 48 ~ 59% inhibition of melanin formation compared with the extract of rocky hand or rocky hand + doodle. In addition, the melanin inhibition rate of arbutin used as a whitening agent 48.3%), respectively.

Experimental Example 2 Evaluation of skin irritation degree

10 to 50 males and 10 females were subjected to a sensory evaluation after using the cream of Preparation Example 1 or the cream containing 60% by weight of Comparative Example 4 continuously for 12 weeks twice a day for 12 weeks .

Each score was assessed as a 5-point scale (1-very poor, 2-poor, 3-moderate, 4-excellent, 5-excellent) for skin moisturization, gloss, elasticity, (In the case of a stimulus, the closer to the 5th point the less stimulus is).

Moisturizing property burnish elasticity Stimulation Whitening The cream of Preparation Example 1 4.6 4.3 4.1 4.7 4.7 The cream containing Comparative Example 4 3.7 3.9 2.9 3.5 3.9

As a result of the sensory evaluation, it was confirmed that the cream containing the rock and hand fermented product had better skin moisturizing, shine, elasticity and whitening effect than the cream containing the rock and hand extracts of Comparative Example 4, .

Experimental Example  3. Skin tone  Improvement efficacy

The sensory evaluation was carried out after 5 creams of 20 to 40 years old women were continuously using the cream of Preparation Example 1 twice a day for 12 weeks. The assessments were evaluated by a professional researcher on items such as skin tone, number of spots, and size of the largest spots.

division Skin tone
(1 point (bright) - 5 points (dark)) Number of spots Size of largest spot
(mm) Before processing After processing Before processing After processing Before processing After processing Subject 1 3.8 2.5 5 2 2.2 1.8 Subject 2 3.2 1.9 5 2 2.7 1.6 Target person 3 3.2 1.7 3 0 1.9 0.5 Subject 4 4.9 3.2 7 2 3.7 1.8 Subject 5 5.0 2.7 7 5 3.0 1.5

As a result of the sensory evaluation, it was confirmed that the skin tone was brightened in all the subjects after the treatment, and both the number of spots and the size of the largest spots were reduced, confirming the whitening effect of the cosmetic composition according to the present invention.

Experimental Example  4. Clinical application to patients with skin diseases

The cream formulations of Preparation Example 1 according to the present invention were actually applied to people with various skin disease states. Actual photographs prepared before and after the treatment of Production Example 1 according to the present invention were recorded (Figs. 2A to 2E). After using the cream according to the present invention, it is possible to visually confirm that the condition of the skin disease is remarkably improved by the effect of skin whitening, moisturizing, gloss enhancement and elasticity enhancement.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (10)

A method for preparing a whitening cosmetic composition, which comprises fermenting a rocky hand fermentation broth as an active ingredient and the above-mentioned rocky hand fermentation broth with a lactic acid bacterium (Lactobacillus bacteria), a bifidobacteria or a mixture thereof .
The method according to claim 1,
Wherein said rocky hand fermentation broth is obtained by inoculating Lactobacillus bacteria, Bifidobacteria or a mixture thereof, and then fermenting at 30 to 40 DEG C for 5 to 20 days.
The method according to claim 1,
Wherein the cosmetic composition further comprises a soybean fermentation broth, wherein the soybean fermentation broth is fermented with Lactobacillus bacteria, Bifidobacteria or a mixture thereof.
The method of claim 3,
Wherein the soybean fermentation broth is obtained by inoculating Lactobacillus bacteria, Bifidobacteria or a mixture thereof and then fermenting at 30 to 40 DEG C for 5 to 20 days.
5. The method according to any one of claims 1 to 4,
The cosmetic composition of the present invention may be used in cosmetics such as astringent lotion, supple lotion, skin lotion, cream, essence, nutrition gel, lipstick, foundation, eye shadow, pack, patch, mast sheet, soap, shampoo, rinse, Wherein the composition has a formulation selected from the group consisting of cleansing cream, body lotion and body cleanser.
a) sterilizing the rocks by heating them at 95 to 130 ° C for 5 to 30 minutes;
b) adding Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rocks and fermenting them;
and c) extracting the fermented rock hands.
a) sterilizing the rock hands and horses by heating at 95 to 130 ° C for 5 to 30 minutes;
b) adding the Lactobacillus bacteria, Bifidobacteria or a mixture thereof to the sterilized rock hands and horses;
and c) extracting the fermented rock hands and toes. delete delete delete

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