KR102142930B1 - A composition for promoting melanin synthesis comprising flower extract of milk thistle - Google Patents

Abstract

The present invention relates to a composition for promoting melanin production comprising a milk thistle flower extract as an active ingredient, and the composition of the present invention comprising a milk thistle flower extract promotes melanin production and induces blackening to induce vitiligo, It has the effect of preventing, improving or treating gray hair or hypochromatism. In addition, the composition has no skin irritation and cytotoxicity, has excellent human stability, and is very effective in promoting melanin production, so it can be safely used as an active ingredient in cosmetics or pharmaceutical compositions for the prevention, improvement or treatment of vitiligo, white hair or hypopigmentation. I can.

Description

A composition for promoting melanin production comprising a flower extract of milk thistle as an active ingredient {A composition for promoting melanin synthesis comprising flower extract of milk thistle}

The present invention relates to a composition for promoting melanin production comprising a milk thistle flower extract as an active ingredient, and more specifically, to promote melanin production and induce blackening to cause vitiligo, gray hair, or hypopigmentation ( It relates to a cosmetic or pharmaceutical composition comprising a milk thistle flower extract capable of preventing, improving or treating hypochromatism) as an active ingredient.

Melanin, produced by melanocytes in the base layer of the epidermis of human skin, refers to a phenolic polymer that has a complex form of black pigment and protein. The melanin determines the color of a person's skin according to its concentration and distribution, and also serves to protect the human skin from ultraviolet rays.

When melanin is produced excessively, it causes pigmentation such as spots and freckles. On the contrary, if the production of melanin is excessively suppressed, skin lesions of vitiligo may appear, the number and function of melanocytes in the hair root may decrease, resulting in white hair, and hypopigmentation may occur, which is a risk factor for skin cancer. Therefore, studies that promote melanin production and induce blackening are very important as much as studies related to whitening that inhibit melanin production.

These vitiligo, white hair, and hypopigmentation are not only not looking good in appearance, but also cause many difficulties in social activities. Therefore, in the case of vitiligo and hypopigmentation, there are many cases where they are treated using surgical procedures or temporarily concealed using external skin preparations such as cosmetics, and many people are trying to temporarily hide gray hair by dyeing white hair. However, since these methods are not fundamental prevention, improvement or treatment methods, studies on melanin production accelerators are necessary for a more fundamental solution.

In this regard, Korean Patent Registration No. 10-1669359 discloses a composition for promoting melanin synthesis comprising a mixture of azalea extract, salicornia extract, and oyster oak extract, but since each extract is used by mixing several extracts There is a problem that the preparation of raw materials and the manufacturing process are complicated.

On the other hand, milk thistle is a perennial plant of the genus Asteraceae white-patterned thistle. It is a perennial plant or a biennial plant whose leaves have clear white stripes. The generic name'Silybum' is derived from the Greek name for Dioscorides attached to a thistle-like plant, and it is widely known as milk thistle because when the leaves are broken, milky sap comes out. Milk thistle extract is known to be effective in not only improving liver function, but also reducing total cholesterol and treating diabetes, and is also used to prevent motion sickness and heart disease. However, as of yet, the effect of promoting melanin synthesis of milk thistle flower extract has not been found.

Accordingly, the inventors of the present invention are researching natural materials that can effectively promote melanin production with little stimulation to the human body in order to fundamentally solve vitiligo, white hair and hypopigmentation in addition to the above problems, milk thistle flower extract is melanin. The present invention was completed by promoting production to induce blackening, and confirming that the human body has excellent stability due to lack of skin irritation and cytotoxicity.

The technical problem to be solved in the present invention is to provide a composition for promoting melanin production comprising a milk thistle flower extract as an active ingredient.

Another technical problem to be solved in the present invention is to provide a cosmetic composition for preventing or improving vitiligo, white hair, or hypopigmentation comprising a milk thistle flower extract as an active ingredient.

Another technical problem to be solved in the present invention is to provide a pharmaceutical composition for preventing or treating falsification, white hair, or hypopigmentation comprising a milk thistle flower extract as an active ingredient.

In order to solve the above technical problem, the present invention provides a composition for promoting melanin production comprising a milk thistle flower extract as an active ingredient.

The milk thistle flower extract according to the present invention promotes the production of melanin, which is a pigment cell, and increases the synthesis of melanin by activating the produced melanocytes, thereby causing pigment re-deposition (repigmentation).

In order to solve the above other technical problems, the present invention provides a cosmetic composition for preventing or improving vitiligo, white hair or hypopigmentation comprising the composition for promoting melanin production.

In order to solve the above other technical problems, the present invention provides a pharmaceutical composition for preventing or treating vitiligo, white hair or hypopigmentation comprising the composition for promoting melanin production.

The milk thistle used in the present invention is Silybum marianum or Silybum ebumeum , Silybum marianum means blessed milk thistle, and is found throughout Europe, Asia, North Africa, China, Australia, etc., Silybum eburneum is The silver milk thistle (silver milk thistle) or elephant thistle (elephant thistle) is mainly originated from Algeria, Morocco and Spain. All parts are edible, and mainly leaves, stems, roots, and seeds are used. In particular, silymarin in the seeds has a strong antioxidant function and is known for its ability to detoxify and protect the liver from many toxins in the human body. .

The term "extract" of the present invention refers to a preparation obtained by squeezing a herbal medicine into an appropriate leachate and evaporating the leachate, but is not limited thereto, but is not limited to the extract obtained by the extraction treatment, the dilution or concentrate of the extract, or the extract. It may be a dried product obtained by drying, a preparation or purified product thereof.

The milk thistle flower extract of the present invention may be extracted using a conventional solvent known in the art, and the extracted liquid may be used in a liquid form or concentrated and/or dried. At this time, as the solvent, water, an anhydrous or aqueous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform, or 1,3 -Any one of butylene glycol can be used. Preferably, it can be extracted using methanol, ethanol or butanol. However, it is not particularly limited thereto, and since the degree of extraction and loss of the active ingredient of the extract may vary depending on the organic solvent to be extracted, it is preferable to select and use an appropriate organic solvent.

The extraction method is not particularly limited thereto, but includes cold needle extraction, ultrasonic extraction, or reflux cooling extraction method, and it is preferable to perform ultrasonic extraction.

In addition, the milk thistle flower extract may be obtained through a conventional purification process in addition to the method of extracting with the extraction solvent. For example, through various additional purification methods, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity, or affinity). Milk thistle flower extract can also be obtained through the obtained fraction.

According to one specific embodiment of the present invention, the milk thistle flower is shaded and ground, and then 0.5 to 20 times the weight of the dried sample, preferably 1 to 15 times the amount of water, ethanol, methanol, etc. Using a lower alcohol of C4 or a mixed solvent of water and ethanol at room temperature for about 20 to 70 hours, preferably 40 to 50 hours, an extraction method such as stirring extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction. Using, preferably, the extract obtained after ultrasonic extraction may be filtered, concentrated under reduced pressure, or dried to obtain a milk thistle flower extract.

The milk thistle flower extract of the present invention may be all extracts, fractions, purified products, diluted solutions, concentrates, or dried products obtained in each step of extraction, fractionation, or purification.

The composition for promoting melanin production comprising the milk thistle flower extract of the present invention as an active ingredient comprises 0.0001 to 20.0% by weight, preferably 0.001 to 10.0% by weight of the milk thistle flower extract, based on the total weight of the composition. . If the content of the milk thistle flower extract is less than 0.0001% by weight, the effect of promoting melanin production cannot be expected, and if it exceeds 20.0% by weight, the increase in the effect of promoting melanin production according to the content increase is insignificant, and it is difficult to prepare a pharmaceutical formulation. Occurs.

In one specific embodiment, the composition for promoting melanin production comprising the milk thistle flower extract of the present invention as an active ingredient is used to prevent, improve or treat vitiligo, white hair, or hypopigmentation caused by destruction or dysfunction of melanocytes. It can be used as a cosmetic composition or a pharmaceutical composition for.

The vitiligo is a phenomenon in which melanocytes of various sizes and shapes appear on the skin due to the destruction of melanocytes due to an abnormality in autoimmune function, which means an acquired depigmenting disease.

The gray hair refers to a phenomenon in which the hair turns white while the number and function of melanocytes in the hair root decreases.

The hypochromatism refers to a disease in which a functional disorder occurs in melanocytes locally due to skin diseases such as eczema, atopic dermatitis, and inflammatory dermatitis, so that the skin at the lesion site temporarily turns white.

In the present invention, the prevention, improvement or treatment of vitiligo, white hair, or hypopigmentation is to stimulate inactive melanocytes or pigment blasts present in the skin or hair follicles to promote differentiation, proliferation and migration, and to produce melanin to produce vitiligo, To prevent, ameliorate or treat symptoms of white hair or hypopigmentation.

More specifically, the present invention provides a cosmetic composition effective in preventing or improving vitiligo, white hair, or hypopigmentation by promoting the production of melanin in cells by including the milk thistle flower extract as an active ingredient.

The cosmetic composition comprising the milk thistle flower extract of the present invention as an active ingredient contains 0.0001 to 20.0% by weight, preferably 0.001 to 10.0% by weight of the milk thistle flower extract, based on the total weight of the cosmetic composition. When the content of the milk thistle flower extract is less than 0.0001% by weight, no effect can be expected in the prevention or improvement of vitiligo, white hair, or hypopigmentation, and when it exceeds 20.0% by weight, it is difficult to prepare a cosmetic formulation.

The cosmetic composition may additionally include components commonly used in cosmetic compositions in addition to the milk thistle flower extract. For example, it may contain conventional auxiliaries and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray. More specifically, it may be prepared in a formulation such as a nourishing cream, an astringent lotion, a flexible lotion, a lotion, an essence, a nourishing gel or a massage cream. However, it is not particularly limited thereto.

When the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Can be used.

When the formulation of the cosmetic composition is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, Propellants such as propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan can be used. have.

When the formulation of the cosmetic composition is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

When the formulation of the cosmetic composition is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acids as carrier components Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

More specifically, the present invention provides a pharmaceutical composition effective in preventing or treating vitiligo, white hair or hypopigmentation by promoting the production of melanin in cells by including the milk thistle flower extract as an active ingredient.

The pharmaceutical composition comprising the milk thistle flower extract of the present invention as an active ingredient contains 0.0001 to 20.0% by weight, preferably 0.001 to 10.0% by weight of the milk thistle flower extract, based on the total weight of the cosmetic composition. If the content of the milk thistle flower extract is less than 0.0001% by weight, the preventive or therapeutic effect of vitiligo, white hair, or hypopigmentation cannot be expected, and if it exceeds 20.0% by weight, the increase in the effect due to the increase in the content is insignificant and pharmaceuticals Difficulties arise in preparing the formulation.

In addition to the milk thistle flower extract, the pharmaceutical composition may further include a carrier generally acceptable for pharmaceutical compositions.

Carriers that are generally acceptable for the pharmaceutical composition are those commonly used in formulation, and carbohydrate compounds (e.g., lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch, cellulose, etc.), gum acacia, phosphoric acid Calcium, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oil (e.g. corn oil, cotton seed oil, soy milk, olive oil, Coconut oil), polyethylene glycol, methyl cellulose, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate, mineral oil and the like, but are not limited thereto.

The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be applied to mammals such as rats, mice, livestock, humans, etc. by oral or parenteral administration, preferably parenteral administration, more preferably topical application by application. It can be applied in a way.

A suitable dosage of the pharmaceutical composition may be prescribed in various ways depending on factors such as formulation method, mode of administration, age, weight, sex, pathological condition, food, administration time, route of administration, excretion rate and response sensitivity of the patient. have. The oral dosage of the pharmaceutical composition of the present invention is in the range of 0.001-300 mg/kg (body weight) on an adult basis. In addition, in the case of an external preparation, it is good to apply for 1 to 5 times a day in an amount of 1.0 to 3.0 ml on an adult basis and continue for at least 1 month. However, the dosage is not intended to limit the scope of the present invention.

The cosmetic or pharmaceutical composition containing the milk thistle flower extract of the present invention as an active ingredient promotes the production of melanin in an individual by intra-individual administration, subcutaneous administration, or topical administration by application, and by activation of the produced melanocytes, melanin There is an advantage in that the synthesis is increased to cause pigment re-deposition (repigmentation), thereby achieving the purpose of alleviating, ameliorating, preventing, improving or treating vitiligo, white hair or hypopigmentation.

In one specific embodiment, the present invention provides a method for improving vitiligo, white hair, and hypopigmentation in an individual by administering a composition comprising a milk thistle flower extract as an active ingredient to an individual to promote melanin production in the individual, and its use. to provide.

The composition administered to an individual of the present invention has an effect of improving or treating vitiligo, white hair, and hypopigmentation of the individual due to the effect of promoting melanin production as described above.

In the present invention, the term "subject" refers to monkeys, cows, horses, pigs, sheep, dogs, cats, rats, mice, including humans with conditions or diseases in which symptoms can be improved by administering the composition of the present invention, It means mammals such as chimpanzees.

In the present invention, the term "improvement" refers to (a) inhibition of development, (b) alleviation, (c) elimination of vitiligo, white hair or hypopigment caused by destruction or dysfunction of melanocytes in an individual.

On the other hand, the milk thistle flower extract of the present invention is a natural extract that does not have skin irritation and cytotoxicity and has little side effects to the human body due to excellent human stability and is very effective in promoting the production of melanin, thus preventing vitiligo, white hair or hypopigmentation. It can be safely used as an active ingredient in cosmetic or pharmaceutical compositions for improvement or treatment.

The composition for promoting melanin production, including the milk thistle flower extract of the present invention as an active ingredient, has no skin irritation and cytotoxicity, has excellent human stability, and is very effective in promoting melanin production, thus preventing and improving vitiligo, white hair or hypopigmentation. Or it can be safely used as an active ingredient in therapeutic cosmetics or pharmaceutical compositions.

1 is a graph showing the effect of promoting melanin synthesis according to the concentration of the extract for each portion of milk thistle in human melanocytes.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1: Preparation of two kinds of milk thistle extracts for each part

Two kinds of milk thistle ( Silybum marianum , Silybum ebumeum ) were collected by flowers, leaf stems and roots at the top. Flowers were prepared separately from the seed part. The collected area was cleaned to completely remove foreign substances and impurities, dried in the shade at 20 to 35° C., and then pulverized so that the size of the particles was 1 mm or less. Thereafter, 1 kg of the pulverized milk thistle powder was immersed in 70% ethanol solvent, followed by ultrasonic extraction for 48 hours, and then the obtained extract was filtered using a filter paper (Advantes, No. 2). Then, it was concentrated under reduced pressure to prepare an extract for each portion of the milk thistle 2 species.

Example 2: Measurement of the effect of promoting melanin production by extracts of two types of milk thistle in human melanocytes

In order to investigate the effect of promoting melanin production by the milk thistle extract according to the present invention, the following experiment was performed using human melanocytes.

Human melanocytes were inoculated at 1.5x10 ⁵ per well in a 6-well plate using M254 medium containing HMGS (Human Melanocytes Growth Supplement), until the cells adhered to the bottom of the well by about 80% or more. Incubated at 5% CO ₂ and 37°C. After cultivation, the medium was removed, and each sample obtained in Example 1 was replaced with a medium diluted to an appropriate concentration, followed by incubation at 5% CO ₂ and 37°C for 5 days. The concentration range of the extract for each portion of the milk thistle according to Example 1 was determined to be 1 ppm, 3 ppm, and 5 ppm without cytotoxicity. The cells from which the medium was removed were washed with PBS (phosphated buffer salinee), and then the cells were recovered. For the recovered cells, the number of cells was measured using a hemocytometer, and then centrifuged at 10,000 to 13,000 rpm for 10 minutes to remove the supernatant and obtain a pellet. After drying the obtained cell pellet at 60°C, 100 μl of 1M sodium hydroxide solution containing 10% DMSO was added to obtain an intracellular melanin solution in a 60°C incubator. With this solution, absorbance was measured at 450 nm with a microplate reader to determine the amount of melanin per cell. The results are shown in Table 1 and Fig. 1 below.

1 is a graph showing the effect of promoting melanin synthesis according to the concentration of the extract for each portion of milk thistle in human melanocytes.

sample part Treatment concentration (μg/mL) Melanin production promotion rate (%) control (-) - - 100 ± 3.8 Silybum
marianum Flower One 167 ± 0.7 5 200 ± 3.3 Leaf/stem One 104 ± 0.7 5 103 ± 2.2 Root One 100 ± 1.5 5 100 ± 2.2 Silybum
ebumeum Flower One 179 ± 1.8 5 199 ± 2.0 Leaf/stem One 101 ± 1.8 5 104 ± 0.2 Root One 103 ± 0.4 5 104 ± 0.2

As shown in Table 1 and FIG. 1, as a result of the test of the melanin production promoting effect of the milk thistle extract for each part, the flower extract for the two types of milk thistle had a higher rate of promoting melanogenesis compared to the control group in a concentration-dependent manner, and 5 ㎍/ When treated with a concentration of mL, it was observed that the melanin production was more than doubled. However, the extracts other than the flower extract did not show the effect of promoting melanin production.

Example 3: Test for safety confirmation of two types of milk thistle flower extracts on human skin

In order to confirm whether the two types of milk thistle flower extracts according to the present invention are safe for human skin, a skin safety verification experiment was performed. For this, a cumulative skin irritation test was conducted.

A nutrient cream containing 0.1%, 0.5%, and 1% of the two types of milk thistle flower extracts prepared in Example 1, respectively, was prepared. Specifically, purified water, triethanolamine and propylene glycol, which are aqueous phases, were heated to 70°C and dissolved, and then beeswax, liquid paraffin, oily components, emulsifiers and preservatives were heated to 70°C, and the dissolved solution was added to emulsify. After the emulsification was completed, the solution was cooled to 45° C., and 0.1%, 0.5%, and 1% milk thistle flower extract were added, dispersed, and then cooled to 30°C. As the content of the milk thistle flower extract was increased to 0.1%, 0.5% and 1%, the amount of liquid paraffin was reduced to 9.91%, 9.51% and 9.01%, respectively, to prepare a nutrient cream.

Using the nutritional cream prepared as described above, a total of nine 24-hour cumulative patches were applied on the upper forearm every other day for 30 healthy adults to determine whether the milk thistle flower extract irritated the skin.

The patching method used a pin chamber (Finn chamber, Epitest Ltd, Finland). After 15 µl of each of the external preparations for skin was added dropwise to the chamber, a patch was applied. The degree of reaction that appeared on the skin each time was scored using Equation 1 below, and the results are shown in Table 2 below.

In the reactivity, ± is 1 point, + is 2 points, and ++ is 4 points, and when the average reactivity is less than 3, it is judged as a safe composition.

Test substance Number of subjects with a reaction Average
Responsiveness 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + Squalene
[Control] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 Silybum
marianum 0.1%
[Test group 1] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 0.5%
[Test group 2] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 One%
[Test group 3] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 Silybum
ebumeum 0.1%
[Test group 4] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 0.5%
[Test group 5] 0 - - 0 - - --- --- --- --- --- --- --- 0.00 One%
[Test group 6] 0 - - 0 - - --- --- --- --- --- --- --- 0.00

As shown in Table 2, the number of people corresponding to ±, + and ++ in all of the test groups 1 to 6 was 0, and the average response was also 0.00. As a result of the above test, since the average reactivity was 3 or less, the milk thistle flower extract according to the present invention was determined to be a safe substance for human skin that does not exhibit a distinct cumulative irritation pattern.

The composition of the present invention may be prepared as a formulation example as follows, but is not limited thereto.

Formulation Example 1: Cosmetic formulation

1-1. Flexible lotion

As shown in Table 3 below, flexible cosmetic water containing the milk thistle flower extract as an active ingredient was prepared according to a conventional method.

ingredient weight% 2 types of milk thistle flower extract 0.01 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, trace pigments, trace flavors and trace purified water 82.79 sum 100.0

1-2. Nutritional lotion

As shown in Table 4 below, nutrient cosmetic water containing the milk thistle flower extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Milk Thistle Flower Extract 0.01 Beeswax 4.0 Polysorbate 60 1.5 Sorbitansquioleate 0.5 Floating paraffin 5.0 Squalane 5.0 Caprylic/Capric Triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservatives, trace pigments, trace flavors and trace purified water 69.69 sum 100.0

1-3. Nourishing cream

As shown in Table 5 below, a nutritional cream containing the milk thistle flower extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Milk Thistle Flower Extract 0.01 Beeswax 10.0 Polysorbate 60 1.5 Sorbitansquioleate 0.5 Floating paraffin 10.0 Squalane 5.0 Caprylic/Capric Triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, trace pigments, trace flavors and trace purified water 56.79 sum 100.0

1-4. Massage cream

As shown in Table 6 below, a nourishing cream containing the milk thistle flower extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Milk Thistle Flower Extract 0.01 Beeswax 10.0 Polysorbate 60 1.5 Sorbitansquioleate 0.8 Floating paraffin 40.0 Squalane 5.0 Caprylic/Capric Triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, trace pigments, trace flavors and trace purified water 27.49 sum 100.0

1-5. pack

As shown in Table 7 below, a pack containing the milk thistle flower extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Milk Thistle Flower Extract 0.01 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonylphenyl ether 0.3 Preservatives, trace pigments, trace flavors and trace purified water 81.39 sum 100.0

Formulation example 2: pharmaceutical preparation

2-1. Preparation of powder

ingredient Content (g) Milk Thistle Flower Extract 2 Lactose One

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

2-2. Preparation of tablets

ingredient Content(mg) Milk Thistle Flower Extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above ingredients, tablets were prepared by tableting according to a conventional tablet preparation method.

2-3. Preparation of capsules

ingredient Content(mg) Milk Thistle Flower Extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

After mixing the above ingredients, a gelatin capsule was filled according to a conventional capsule preparation method to prepare a capsule formulation.

Claims (5)

A composition for promoting melanogenesis, comprising a flower extract from which seeds are removed from milk thistle flowers. The method of claim 1,
The milk thistle is Silybum marianum or A composition for promoting melanin production, characterized in that it is Silybum ebumeum . A cosmetic composition for preventing or improving vitiligo, white hair or hypopigmentation comprising the composition for promoting melanin production according to claim 1 or 2. The method of claim 3,
The cosmetic composition is any one of the group consisting of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. A cosmetic composition for preventing or improving vitiligo, white hair or hypopigmentation, characterized in that. Vitiligo, white hair or hypopigmentation prevention or treatment pharmaceutical composition comprising the composition for promoting melanin production according to claim 1 or 2.

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