KR20190020946A - A composition for preventing or improving skin wrinkle comprising herb extracts or fermentation products thereof - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating skin wrinkles, comprising herbal extracts or fermented products thereof. The composition, comprising the herbal extracts, which consist of Rehmannia glutinosa, Astragalus membranaceus Bunge, green tea, peony, Cornus officinalis, Pueraria lobata Ohwi, Schisandra chinensis, Angelica gigas, Cnidium officinale Makino, cinnamon, Illicium vernum and placenta diffusa, or the fermented products thereof, honey and a cosmetically, pharmaceutically or cosmetically acceptable salt thereof, as active ingredients, has excellent collagen biosynthetic ability and an excellent inhibitory effect on collagenase, thereby having a skin wrinkle alleviating effect. In addition, the herbal extracts consisting of Rehmannia glutinosa, Astragalus membranaceus Bunge, green tea, peony, Cornus officinalis, Pueraria lobata Ohwi, Schisandra chinensis, Angelica gigas, Cnidium officinale Makino, cinnamon, Illicium vernum and placenta diffusa or the fermented products thereof and honey as the active ingredients of the composition for preventing or alleviating skin wrinkles, have no cytotoxicity and skin side effects and thus can be safely used in cosmetic compositions, pharmaceutical compositions and food compositions.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing or improving skin wrinkles comprising a herbal medicine extract or a fermented product thereof. BACKGROUND OF THE INVENTION 1. Field of the Invention < RTI ID = 0.0 &

The present invention relates to a composition for prevention or improvement of skin wrinkles comprising a medicinal herb extract or a fermented product thereof, and more particularly to a composition for preventing or improving wrinkles of a skin including a medicinal herb extract or a fermented product thereof. The present invention also relates to a cosmetic composition, a pharmaceutical composition and a food composition for prevention or improvement of skin wrinkles containing an extract of a donkey placenta, fermented extract thereof and honey as an active ingredient.

Skin is the primary organ of aging, and much research has been done in the field of cosmetics to delay or prevent it. Skin aging usually begins around the age of 25, and skin aging proceeds in earnest at around the age of 40 years. As a representative phenomenon of skin aging, sebum secretion is reduced, skin becomes dry, cell regeneration is delayed, and aging skin is piled up and skin becomes rough. In addition, the amount of collagen that supports the epidermis is reduced, and elastin (elastic fibers) is denatured to form wrinkles. In addition, the color of the skin is stained, spots, black spots and pigmentation symptoms appear, the skin becomes thinner and the skin protection function is weakened. Other phenomena include an increase in skin troubles due to a decrease in the skin barrier effect (barrier effect) associated with a decrease in skin thickness. Researches related to skin aging are classified into materials that protect skin from ultraviolet rays and active oxygen, which are major causes of skin aging, skin wrinkle improving materials, skin elasticity enhancing materials, skin moisturizing materials, have.

Dermatological atrophy is a typical aging phenomenon after 70 years of age. Changes in the dermis are caused by a change in the number of fibroblasts and a change in the molecular weight of the extracellular matrix due to a decrease in their ability to synthesize. Specific changes include separation of collagen bundles, reduction of point polysaccharide synthesis, reduction in the number and diameter of collagen and elastic fibers, collagen and elastic fiber grinding, and expansion of blood vessels. Generally, the most important factors affecting the formation of wrinkles among various complex factors such as moisture content of the skin, collagen content, and immunity against the external environment are collagen decomposition enzyme collagenase, which reduces collagen production and collagen content The amount and activity of digestive enzymes.

Therefore, in the field of cosmetics, research and development on functional cosmetics having the effect of improving the wrinkles of the skin are actively carried out, and studies using natural materials have been continuing in order not to give toxicity or irritation to the skin.

Accordingly, the present inventors have sought to develop a naturally occurring compound which has excellent skin wrinkle-reducing effect and skin aging-preventing effect, has high safety and stability, and has no skin side effects. As a result, it has been found that a composition comprising Sukjwanghwang, Hwanggi, green tea, peony, corn oil, Puerariae, Omija, Angelica gigantei, cinnamon, cinnamon and donal placenta extract or extract fermented products thereof increases collagen synthesis and inhibits collagenolytic enzyme activity The present invention has been completed.

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a composition for preventing or improving wrinkles of a skin having excellent collagen synthesis ability and excellent collagenase activity inhibiting action.

In order to solve the above-mentioned technical problems, the present invention provides a mixed extract or a fermented product consisting of Sukjunghwang, Hwanggi, green tea, peony, Sansui, Pueraria, Omija, Angelica gigas, Or a pharmaceutically acceptable salt thereof, for the prevention or amelioration of skin wrinkles.

In the present invention, skin wrinkles comprising a mixed extract consisting of Sukjunghwang, Hwanggi, Green tea, Peony root, Sansui, Pueraria, Omija, Angelica, Angelica, Cinnamon, Prevention or amelioration of a disease or condition.

In the present invention, skin wrinkles containing mixed extracts or a fermented product thereof, honey and a pharmaceutically acceptable salt thereof, consisting of a mixture of Sukjunghwang, Hwanggi, Green tea, Peony root, Sansui, Pueraria, Omija, Angelica gigas, Prevention or amelioration.

According to one preferred embodiment, in the present invention, 2 to 10% by weight of steamed rice, 2 to 10% by weight of sulfur, 2 to 10% by weight of green tea, 2 to 10% by weight of peanut, 1 to 8% 1 to 3% by weight of honey and 1 to 3% by weight of honey and 40 to 60% by weight of honey, 0.5 to 5% By weight of a composition for prevention or improvement of skin wrinkles.

In the present invention, the " extract " is an extract obtained by extracting each of the above-mentioned Sukjunghwang, Hwanggi, Green tea, Peony, Sansui, Punggi, Omija, Angelica gigas, , Peony root, corn oil, Puerariae radix, Omija, Angelicae gigantei, cinnamon, cinnamon, garlic, and donkey placenta, as well as their dry powders or all forms thereof formulated therewith.

The extract can be extracted with water or an organic solvent, and the extracted liquid can be used in liquid form or can be used by concentration and / or drying. The organic solvent may be selected from the group consisting of methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethylsulfoxide , 3-butylene glycol, propylene glycol, or a mixed solvent thereof. The extract can be extracted at room temperature or with heating under the condition that the active ingredient of the extract is not destroyed or minimized.

In one specific embodiment, the extracts of Sukjwanghwang, Hwanggi, green tea, peony, Sansui, Pueraria, Omija, Angelica, Angelica, Cinnamon, After extracting the time, the process of collecting the supernatant with the filter paper is repeated 3 times, and then collected and dried under reduced pressure.

The extraction method is not limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction.

The mixed extracts of the present invention of the present invention can be obtained by extracting all extracts obtained in each step of extraction, fractionation, or purification (separation, fractionation) Fraction, purified product, diluted solution thereof, concentrated solution, or dried product.

In the present invention, all parts such as stem, root, leaf, and flower can be used as extraction sites of Sukjunggwang, Hwanggi, Green tea, Peony root, Sansui, Puerariae, Omija, , And is not limited to any specific site.

In the present invention, " extracted fermented product " means that the extract is fermented in lactic acid bacteria. In this case, lactic acid bacteria are used as the fermenting bacteria, for example, Lactobacillus casei casei, L. plantarum , L. acidophilus , L. bulgaricus , Bifidobacterium longum , Bifidobacterium, Bifidobacterium longum , Bifidobacterium longum , B. bifidum or a mixed microorganism thereof may be used. Preferably, L. plantarum, B. bifidum or a mixed microorganism thereof can be used. And L. plantarum and B. bifidum are particularly preferable.

In a preferred embodiment of the present invention, the fermented extract is prepared by adding lactic acid bacterium to a mixed extract of Sukjunghwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica, By weight, and incubating for 10 to 20 hours.

The mixed extract of the present invention or a fermented product thereof and honey or its cosmetic, pharmacologically or pharmacologically acceptable extracts of the present invention, A composition for improving skin wrinkles containing a salt as an active ingredient has a use for preventing skin aging or improving skin wrinkles.

The composition of the present invention exhibits an excellent wrinkle-reducing effect through the promoting mechanism of collagen synthesis and collagenase inhibiting activity while being low in cytotoxicity, which is clearly confirmed by the following examples.

The improvement in skin wrinkles, which is the application of the composition of the present invention, is interpreted to include conventional skin protection uses (prevention of wrinkles, removal of wrinkles, prevention or treatment, etc.).

In a specific embodiment of the present invention, collagen biosynthesis, collagenase activity inhibitory activity and the like, which are generally used for screening wrinkle-reducing substances, are measured. As a result, a composition comprising a mixed extract consisting of Sukjunghwang, Hwanggi, green tea, peony, Sansui, Pueraria, Omija, Angelica, Angelica, Cinnamomum, Convolvulus and Don placenta or a fermented product thereof and honey has the advantages of promoting collagen production and cell proliferation But also the effect of inhibiting collagenase activity can be observed.

The active ingredient of the composition of the present invention, rosewood, is the root of Rehmannia glutinosa Liboschitz var. Purpurea Makino or R. glutinosa Liboschitz . It is used for the treatment of blood deficiency and consequently liver and heart malnutrition, It is known to strengthen the height of the kidneys. Symptoms such as back pain, dizziness, dizziness, tinnitus, hearing loss, periodic fever, and cold sweating may occur due to lack of nutrition of the liver and heart. It is known that it is supplemented with jing (jing in Chinese, essence in English), filling the bone, and it is known that it can alleviate vomiting, wheezing, asthma by the nutritional deficiency of the kidney. Thus, although some effects have been known in the past, there has been no study on the effect of inhibiting collagenase activity.

The active ingredient of the composition of the present invention, Astragalus semblanaceus Bunge , grows in the mountain area, reaches a height of 1m and has fine hairs throughout. Leaves are 6 ~ 11 pairs of lobes, and are odd-numbered birds. Flowers bloom in July and August, 15 ~ 18 ㎜ in length, light yellow, and several flowers hang on a long peduncle, forming a bloom. Root is used as a medicinal ingredient, and its active ingredient is folic acid, choline, and the like. In animal experiments, the excitatory and diuretic effects of the central nervous system were also remarkable. When a large amount of powder was administered to rats, the occurrence of nephritis was inhibited, the proteinuria and cholesterolemia were delayed, and the hypotensive effect was also recognized. It is also widely used for uterine drainage, gastrointestinal tract, gastrointestinal tract, esophagus, and uterine bleeding. It also increases physical strength and increases the tension of whole body muscles.

Green tea, which is an active ingredient of the composition of the present invention, uses leaves of Camellia sinensis as a raw material. The young leaves from the newly bloomed branches are used for tea making, and the leaves are usually picked up three times in May, July and August and the best tea in May is the one. This tea is an evergreen tree that grows well in relatively warm and rainfall areas. In order to produce green tea, the other leaf is immediately heated to destroy the oxidizing enzyme to keep the green, while at the same time evaporating the water to make the leaves dull and cloudy. In recent years, cars are manufactured using steam boilers, crude oil generators, oil boilers, rebuilders, vacuum cleaners, and dryers.

Paeonialactiflora, an active ingredient of the composition of the present invention, is a perennial herb that is a perennial herbaceous herbaceous plant of peonies and peonies. It is about 1m in height and may be divided into a white seed or an apple depending on the flower color. Peony contains ingredients such as aspartic acid and benzoic acid, and headache and toothache to settle. In addition, it eliminates the blood in the body, smoothes the blood circulation, cleanses the blood and is effective for the improvement of the female disease. When taking the peony pills steadily, the stomach is relaxed while suppressing the secretion of stomach to relieve stomach cramps and stomach ulcers. It is also effective for abdominal pain.

Cornus officinalis ( Cornus officinalis ), an active ingredient of the composition of the present invention, is a fruit of the saponin tree which is a deciduous tree in the deep forest. Elliptical nuclei are green at first, but ripen reddish from October to October. Seeds are long oval and have ridges. It has a bitter sweetness and strong acidity. It is harvested after the frost fall in mid-October. The flesh is separated from the seeds and the meat is used as a material for sake, tea and Chinese medicine. The pulp contains the glycosides such as cornin, morroniside, loganin, tannin and saponin and organic acids such as wine, malic and tartaric acid. In addition, it contains vitamin A and a large amount of sugars. Seeds include palmitic acid, oleic acid, linoleic acid, and the like. Among the components, cornin is known to have an excitatory action of parasympathetic nerves. From the past, the pulp was weakened in one batch. According to the "Dongbibogam" and the "Fragrant Bulgari", it is said that it has effects such as strengthening of 阴 阴, 肾 精 and 肾气, and convergence. It is used for medicines such as headache, tinnitus, blood circulation, fever, and menstrual hyperactivity. It is also used for folk remedies such as cold sweat and enuresis. It can also be used as a tea or a drink, and has effects such as stopping sweat and consonant. Sansui is native to Korea and China, and is planted in the middle of South Korea. In Korea, Shandong, Gyeonggi Province, Gyeonggi Province, Gyeonggi Province, and Gyeongsangbukdo, Yusung Gun, and other local products are shipped every year.

The active ingredient of the composition of the present invention, Puerariae Radix , is a dried perennial plant of Pueraria lobata Ohwi , a perennial herb of soybean plants and is grown in mountains of Korea, China and Japan . In Oriental medicine, the various parts of 칡 are classified respectively, and the dried root is called as Pueraria, the flower is Galána, the fruit is Galák or Galil, the leaf is called elliptical, and the vine is called Galáman. . It is used for the purpose of releasing cold muscle, digestive diseases, epigastric diseases, and so on. It helps the spleen and stomach to rise, so it can help to relieve thirst It acts as a branch office.

Schisandra chinensis , an active ingredient of the composition of the present invention, has a ball shape with a diameter of about 1 cm and a deep red color. It contains 1-2 drops of red juice and reddish brown seeds. There are five flavors of sweetness, sourness, bitter taste, salty taste and spicy taste, among which the sour taste is strong. There are two types of omija (north omija), south omija, and black omija. Omiza grows mainly in the area of Taebaek Mountain, Namimiza grows in the southern island region, and black omija grows in Jejudo. It is produced in Korea, Japan, Sakhalin, and China. It contains ingredients such as thiazide, thymidine, citrulline, malic acid, and citric acid, which strengthens the heart, lowers the blood pressure, increases immunity, and is used as a tonic. It strengthens the pulmonary function, and it helps to cure the cough and thirst by the deep sea bream and the geodam function. It puts dried fruit in cold water and puts honey and sugar in the water which is reddish, and drinks it with a drink, and it makes it to eat a leaf or a starch piece. Chestnuts, jujube, and mushrooms are put together and boiled to make tea or soak.

Angelica Gigas , an active ingredient of the composition of the present invention, belongs to the fauna of the persimmon, and has the property to make the five healthy and warm the body, and the body of Angelica gigas regulates blood and the upper part of angiosperms has the effect of blood .

The active ingredient of the composition of the present invention, Cnidium officinale , is a perennial herbaceous perennial herbaceous plant having a height of 40 to 70 cm and is a fragrant plant. It has wound healing, skin regeneration and antibacterial action. In addition, it excellently inhibits melanocyte stimulating hormone activity and is excellent in whitening effect.

Cinnamon, an active ingredient of the composition of the present invention, refers to a bark of Cinnamomum cassia Blume (broiler) or a bark of bamboo flora or flounder, which has been somewhat removed. Broiler tree is an evergreen large tree native to southern China. It is used to collect bark from summer to autumn. It has a peculiar smell and later is slightly mucilage, astringent, flavorless, sweet and temperate. Cinnamon promotes blood circulation, eliminates the coldness of the thighs, promotes appetite, promotes digestion, and is used for limb paralysis. It has been reported that the pharmacological action is to inhibit improving bacteria, to inhibit white spore strain, to act on the surface, and to stimulate saliva and gastric secretion.

The active ingredient of the composition of the invention is the daehoehyang fennel (Foeniculum vulgare ), aka Illicium verum, and the fruit of magnolia and evergreen. This fruit is dried and then powdered and used as a spice. When added to a dish cooked for a long time, such as steamed or simmered in a dish made with duck or pork, it is used to eliminate the bad smell of the main ingredients and to make the taste of the dish taste unique. And diuretic and appetite-promoting action, and abdominal bloating and nausea. In China, it is used as a herbal medicine for the treatment of back pain, constipation, and cystitis, and is used to alleviate pain in acupuncture and acute rheumatism. I also use it to taste cough medicine.

The donor placenta (the placenta of the pig), which is the active ingredient of the composition of the present invention, was found to be surprisingly similar to that of the human placenta among mammalian placentas. The donor placenta is an important component of proteins and various nutrients and DNA and RNA. It has been reported as a source of Bio-Active cytosine which is responsible for differentiation and fetal development.

The placenta is an organs in the uterus of a pregnant mammal, which feeds the fetus through the umbilical cord and sustains life. The placenta is released from the uterus at birth, and the placenta is called postnatal. The placenta contains essential amino acids, melatonin, RNA, DNA and other nucleic acid components, and the antioxidant enzyme SOD (Super Oxide Dismutase). It has been shown to be effective in the treatment of various diseases such as hyaluronic acid, antioxidants, cytocines, placental peptides, insulin-like-growth factor, epidermal growth factors (EGFS) (SCAFS, Senescent Cell Adivating Factors) and cytokines are known to be useful for restoring fatigue and improving immunity. In addition, the placenta has an effect of inhibiting the activity of enzyme tyrosinase, which promotes melanin granulation from tyrosine, thereby inhibiting pigment formation and promoting the release of cells containing melanin pigment, (Mallick S et al., Pigment Cell Res. Feb 18 (1), 25-33, 2005), as well as the effect of preventing pigmentation. In addition, the placenta promotes the action of tissue metabolism by increasing sebaceous gland function to normalize the secretion of sebum, the effect is to reduce or eliminate the fine lines. In addition, the placenta contains fibroblast growth factor (FGF), which promotes the proliferation and regeneration of fibroblasts. Through its activation, it increases the amount of collagen, elastin, and hyaluronic acid, restoring the elasticity and elasticity of the skin, And to improve skin deflection.

As a method of extracting placenta, conventionally, a method of hydrolyzing placenta using strong acid (usually 3N hydrochloric acid) or strong alkali to obtain the product, and placenta extraction method using enzyme have been used. Korean Patent Laid-Open No. 10-2007-0004477 describes a method for producing placenta extract through alkaline hydrolysis. In Korean Patent No. 0642080, a method for producing a placenta extract through plant enzyme and acid hydrolysis is described Japanese Patent Laid-Open Publication No. 2004-097033 describes a method for preparing a placenta extract using proteolytic enzyme. In Korean Patent No. 0642681, a method for producing a placenta extract using a supercritical fluid extraction process .

The mixed extract of the present invention or a fermented product thereof and honey or its cosmetic, pharmacologically or pharmacologically acceptable extracts of the present invention, The composition containing the salt exhibits an effect of improving skin wrinkles, and has little cytotoxicity as a natural substance.

According to one embodiment of the present invention, there is provided a cosmetic composition comprising a mixed extract consisting of Safflower, Hwanggi, green tea, peony, corn oil, Puerariae, Omija, Angelica gigas, A cosmetic composition comprising a salt as an active ingredient is provided.

In a preferred embodiment of the present invention, the medicinal herb extract or its fermented product and honey, which comprise the medicinal plant extract of Sukkiwanghae, Huanggi, Green tea, Peony root, Sansui, Pueraria, Omija, Angelica gigas, May be contained in an amount of 0.00001 to 15.0 wt%, more preferably 0.0001 to 10 wt%, and most preferably 0.0001 to 5 wt%.

The cosmetic composition according to one embodiment of the present invention may contain, in addition to the active ingredient, conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, Can be added.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.

When the formulation of the present invention is a paste, cream or gel, use is made of an animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, tosse, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Castellulose, aluminum metahydroxide, bentonite, agar or tracert, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The pharmaceutical composition according to one embodiment of the present invention is pharmaceutically acceptable in addition to mixed extracts or fermented products thereof consisting of Sukjunghwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica, . The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one usually used at the time of formulation, and includes lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably applied by parenteral administration, more preferably topical application by application.

A suitable dosage of the pharmaceutical composition of the present invention may vary depending on such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate, . The dosage of the pharmaceutical composition of the present invention is in the range of 0.001-100 mg / kg on an adult basis. When the composition is an external preparation, it is preferably applied in an amount of 1.0 to 3.0 ml on an adult basis once to five times a day, and continued for 1 month or more. However, the dose is not intended to limit the scope of the present invention.

The pharmaceutical composition of the present invention may be prepared in unit dosage form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art, Into the container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in an oil or aqueous medium, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

In addition, the food composition according to one embodiment of the present invention may contain, as an active ingredient, a mixed extract consisting of Sukjibo, Huanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica, Angelica, Cinnamon, For example, proteins, carbohydrates, fats, nutrients, flavoring agents and flavors, which are conventionally added in the manufacture of food, as well as honey.

Examples of such carbohydrates are monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, have.

On the other hand, the mixed extracts of the active ingredients of the composition of the present invention, namely, Sukjunggwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica gigas, Toxicity and side effects, it can be safely used even in long-term use, and can be safely applied to cosmetic, pharmaceutical and food compositions as described above.

As described above, the herbal medicine extract, the fermented product thereof, the honey, and the cosmetic, pharmaceutical or foodstuffs of the present invention comprising medicinal plant extracts, medicinal plants extracts of the present invention, green tea, peonies, As an active ingredient, is excellent in collagen biosynthesis, has excellent collagenase inhibiting effect, and has an effect of improving skin wrinkles. The present invention also relates to a medicinal herb extract or its fermented product comprising the active ingredients of a composition for preventing or improving skin wrinkles, such as Sukjunghwang, Hwanggi, Green tea, Peony, Sansui, Punggi, Omija, Angelica gigas, Honey has no cytotoxicity and skin side effects and can be safely used in cosmetic compositions, pharmaceutical compositions and food compositions.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the collagen biosynthesis performance of the composition according to the present invention.
FIG. 2 is a graph showing measured collagenase inhibitory effects of the composition of the present invention. FIG.
FIG. 3 through FIG. 5 are clinical photographs showing the effect of improving the wrinkles of beverages containing the medicinal herb extract according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Preparation of a Composition Containing Extracts of Sukjunghwang, Hwanggi, Green Tea, Peony, Corn Starch, Puerariae Radix, Omija, Angelica, Astragalus, Cinnamon,

(W / v) of distilled water was added to each of the extracts and dried at 90 ° C for 4 hours, and then the supernatant was filtered with filter paper, Respectively. This process was repeated three times and then dried under reduced pressure and stored at -20 ° C.

Each of the obtained extracts and honey were mixed at the composition ratios shown in Table 1 below.

Sookmyung Hwanggi green tea Peony Corn oil Changwon Schisandra Angelica Celestial cinnamon Conversation Money placenta honey weight% 6 6 6 6 4.8 4.8 4.8 3 3 3 0.8 1.8 50

Example 2 Preparation of a Composition Containing Extracted Fermented Product of Sukjunghwang, Hwanggi, Green Tea, Peony, Corn Oil, Paddy Rice, Schizandra chinensis, Angelica, Crown, Cinnamon,

Lactobacillus plantarum (Lactobacillus plantarum ) obtained in Example 1 was added to the extracts of Sulfuric Acid, Hwanggi, Green tea, Peony, Corn oil, Paddy root, Omija, Angelica gigas, In an amount of 5% by weight, and cultured for 12 hours to prepare an extract fermented product.

The extracted fermented products and honey from the above-prepared Sulfuric acid, Hwanggi, Green tea, Peonies, Corn oil, Phellinus linteus, Omija, Angelica gigas, Lt; / RTI >

≪ Test Example 1 > Measurement of collagen biosynthesis performance

The effect of improving the wrinkles of the composition according to the present invention was observed by measuring the collagen biosynthesis performance. Human normal fibroblast was inoculated (2 x 10 ⁵ cells / well) into a 24-well microplate containing DMEM medium and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours . After 24 hours, the medium was removed from each well and the samples prepared in Examples 1 and 2 were treated and then re-cultured for 24 hours. After 24 hours, the cell culture medium was collected to prepare a sample.

The amount of collagen synthesis was measured using a procollagen type I C-peptide (Type I C-peptide, manufactured by Takara Bio Inc.) using a collagen measurement kit (Procollagen Type I C-peptide EIA kit MK101, Takara, Japan) : PICP) was measured. The results are shown in Fig.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the collagen biosynthesis performance of the composition according to the present invention. As can be seen from the results, it was confirmed that the composition according to the present invention increased collagen production in human fibroblasts. Especially, the extract fermented was more effective than the extract.

≪ Test Example 2 > Measurement of collagenase inhibitory effect

Collagenase inhibitory effect was measured to observe the effect of improving the wrinkles of the composition according to the present invention. Human normal fibroblast was inoculated (2 x 10 ⁵ cells / well) into a 24-well microplate containing DMEM medium and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours . After 24 hours, the medium was removed from each well, the samples prepared in Examples 1 and 2 were pretreated, and after 2 hours, UVA (5 J / cm ² ) was treated in each well and re-cultured for 24 hours. After 24 hours, the cell culture medium was collected to prepare a sample.

The collagenase inhibitory effect was measured by using a collagenase assay kit (MMP-1 ELISA kit (QIA55), Merck, Germany) according to the kit instructions. The results are shown in Fig.

FIG. 2 is a graph showing measured collagenase inhibitory effects of the composition of the present invention. FIG. As can be seen from the results, it was confirmed that the composition according to the present invention reduced the amount of collagenase produced from the human fibroblasts, and that the extract fermented was more effective than the extract.

≪ Test Example 3 > Measurement of wrinkle improvement effect of cosmetics

(Step 1) Preparation of nutritional cream

The compositions of the herbal cream extracts or the nutritional cream containing the fermented extracts prepared in Examples 1 and 2 are shown in Table 2 below. The aqueous phase, purified water, triethanolamine and propylene glycol were heated and dissolved at 70 DEG C, and a fatty acid, an oily component, an emulsifier and an antiseptic agent were heated to 70 DEG C to dissolve the emulsified liquid. After the emulsification is complete, the solution is cooled to 45 DEG C, and the fermented extract and fragrance are added and dispersed and then cooled to 30 DEG C. [

ingredient Content (% by weight) Herbal medicine extract or fermented extract thereof 0.01, 0.05, or 1.00 Jojoba oil 5.0 Liquid paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 methylglucose distearate 2.0 Glyceryl stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Arginine 0.3 Carboxyvinyl polymer 0.25 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Balance Sum 100

(Step 2) Observation of wrinkle improvement effect of cosmetics

The effect of improving the wrinkles of cosmetic preparations containing medicinal herb extracts or fermented extracts thereof was measured by clinical tests. Nutritive creams (Preparation Example 1 and Preparation Example 2) each containing 1% of the extract or fermented extract prepared in Step 1 above and a nutritive cream containing purified water as a comparative example were used.

The effect of improving wrinkles was evaluated by measuring changes in skin elasticity. Skin elasticity was measured in 30 healthy women (25 to 35 years old) under the condition that the temperature was kept constant at 24 to 26 DEG C and the humidity was 38 to 40%, and three types of nutritional creams of Production Example 1 and Production Example 2 The nutritional cream of the comparative example was applied to the subject's face twice a day for 3 months, then measured using a cutometer SEM 474 (Courage + Khazaka, Cologne, Germany) And the relative values were compared. The test results are shown in Table 3 below.

Test Items Production Example 1 Production Example 2 Comparative Example Skin elasticity 2.68 2.87 2.31

As shown in Table 3, the cosmetic compositions of Production Examples 1 and 2 of the present invention were much more effective in improving the wrinkles than the cosmetic compositions of the comparative preparation examples. In particular, it was confirmed that the cosmetic composition containing the extract fermented product had very good skin elasticity.

≪ Test Example 4 > Measurement of wrinkle improvement effect of food

(Step 1) Production of beverage

The beverage containing the medicinal herb extracts or fermented extracts prepared in Examples 1 and 2 was prepared as shown in Table 4 below.

Raw material name  Formulation (g) ratio(%) Herbal medicine extract or fermented extract thereof  1.0000 0.4125 Liquid fructose  1.0000  0.4125 Anhydrous citric acid  0.4000 0.1650 Incense of fruit mixture 0.3241 0.1337 Purified salt 0.1081  0.0446 Vitamin C 0.1300 0.0536 Cooling incense 0.0550 0.0227 Potassium chloride  0.0400 0.0165 Sucralose 0.0150 0.0062 Magnesium chloride 0.0100 0.0041 젱 젱  230.3180 95.0156 gun 242.40 100.0000

(Step 2) Observation of improvement of wrinkle of beverage

The effect of improving the wrinkles of beverages containing medicinal herb extracts or fermented extracts thereof was measured through clinical studies. The drink containing the extract prepared in step 1 above was taken once daily for 4 weeks to three adult women aged 62, 61, and 55 years old, and the effect of improving the skin wrinkles was measured.

FIG. 3 through FIG. 5 are clinical photographs showing the effect of improving the wrinkles of beverages containing the medicinal herb extract according to the present invention. As can be seen, it was confirmed that the skin wrinkles were remarkably improved after 4 weeks of ingesting the beverage containing the medicinal herb extract according to the present invention.

<Test Example 5> Confirmation of safety for human skin

In order to confirm the safety of human skin extract or extract fermented product to human skin, skin safety verification experiment was conducted. For this purpose, cumulative skin irritation test was performed. Based on squalene, a formulation containing 1% of each herbal medicine extract or fermented extract was prepared, and then a total of 9 times 24 hour cumulative pellets were applied to 30 healthy adults on the upper forearm every day, Or whether the extracted fermented product stimulates the skin. A pin chamber (Finn chamber, Epitest Ltd, Finland) was used as the deposition method. 15 [mu] l of each of the above external preparations for skin was dropped into the chamber, followed by applying a patch. The degree of the skin reaction on each occasion was scored using the following Equation 1, and the results are shown in Table 5 below.

A score of ±, a +, and a ++ is given as 1, 2, and 4, respectively, and a safe composition is determined when the average degree of reaction is less than 3.

Test substance Number of subjects who responded Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Fourth 5th 6th Seventh 8th car 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ Control group
(Squalene) One - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.09 extract
[Test group 1] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00 Extracted fermented product
[Test group 2] 0 - - 0 - - - - - - - - - - - - - - - - - - - - - - - 0.00

In Table 5, the numbers of persons corresponding to ±, + and ++ in test groups 1 and 2 were 0, 0 and 1, respectively, and the average reactivity was 0.00 and 0.00, respectively. Therefore, the extract of the present invention and the fermented extract according to the present invention were judged to be a substance safe for human skin which does not exhibit a distinct cumulative stimulation pattern, since an average degree of reaction is 3 or less.

Examples of formulations for the composition of the present invention are illustrated below.

Formulation Example 1 Cosmetic preparation

1-1. Manufacture of softening longevity

A lotion lotion containing the herb extract or the fermented extract as an active ingredient in the composition shown in the following Table 6 was prepared according to a conventional method.

ingredient weight% Herbal medicine extract or fermented extract 0.01 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 ethanol 10.0 Arginine 0.08 Preservatives, micronutrients, trace fragrances and trace water 82.79 sum 100.0

1-2. Manufacture of nutrition lotions

Nutritional lotions containing the herbal medicine extract or the fermented extract as an active ingredient in the composition as shown in the following Table 7 were prepared according to a conventional method.

ingredient weight% Herbal medicine extract or fermented extract 0.01 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Arginine 0.15 Preservatives, micronutrients, trace fragrances and trace water 69.69 sum 100.0

1-3. Manufacture of nutrition cream

As shown in Table 8 below, a nutritional cream containing the herb extract or the fermented extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Herbal medicine extract or fermented extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Arginine 0.18 Preservatives, micronutrients, trace fragrances and trace water 56.79 sum 100.0

1-4. Manufacture of massage cream

Massage cream containing the herb extract or the fermented extract as an active ingredient in the composition shown in the following Table 9 was prepared according to a conventional method.

ingredient weight% Herbal medicine extract or fermented extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Arginine 0.2 Preservatives, micronutrients, trace fragrances and trace water 27.49 sum 100.0

1-5. Manufacture of packs

A pack containing the herb extract or the fermented extract as an active ingredient in the composition shown in the following Table 10 was prepared according to a conventional method.

ingredient weight% Herbal medicine extract or fermented extract 0.01 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonyl phenyl ether 0.3 Preservatives, micronutrients, trace fragrances and trace water 81.39 sum 100.0

Formulation Example 2: Preparation of pharmaceutical preparations

2-1. Manufacture of Powder

In order to prepare powders containing the herbal medicine extract or the fermented extract as an active ingredient, the ingredients shown in Table 11 below were mixed and filled in airtight bags to prepare powders.

ingredient g Herbal medicine extract or fermented extract 2 Lactose One

The above components were mixed and packed in airtight bags to prepare powders.

2-2. Manufacture of tablets

In order to prepare tablets containing the medicinal herb extract or the fermented extract as an active ingredient, tablets were prepared by mixing the ingredients listed in Table 12 below and tableting according to a conventional preparation method of tablets.

ingredient mg Herbal medicine extract or fermented extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

2-3. Preparation of capsules

In order to prepare a capsules containing the herbal medicine extract or the fermented extract as an active ingredient, the following ingredients of Table 13 were mixed and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

ingredient mg Herbal medicine extract or fermented extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

A method for preventing or preventing skin wrinkles comprising a mixed extract consisting of Sukjunghwang, Hwanggi, green tea, peony, corn oil, Pueraria, Omija, Angelica gigas, cinnamon, A cosmetic composition for improvement. The method according to claim 1,
2 to 10% by weight of steamed rice, 2 to 10% by weight of green tea, 2 to 10% by weight of green tea, 2 to 10% by weight of peanut, 1 to 8% by weight of corn oil, 1 to 8% by weight of Puerariae, 1 to 8% The prevention or improvement of skin wrinkles comprising 0.5 to 5% by weight of celery, 0.5 to 5% by weight of cinnamon, 0.5 to 5% by weight of cinnamon, 0.1 to 2% by weight of carrot, 1 to 3% by weight of placenta and 40 to 60% &Lt; / RTI &gt; The method according to claim 1,
The extract was extracted with distilled water in an amount of 10 times (w / v) of the weight of each of Sukjunggwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica gigas, And recovering the supernatant with filter paper is repeated three times to collect and then dried under reduced pressure to prevent or improve the wrinkle of the skin cosmetic composition. The method according to claim 1,
The extracted fermented product is prepared by adding lactic acid bacterium to each extract of Sukjiwha, Hwanggi, Green tea, Peony root, Corn oil, Phellinus linteus, Omija, Angelica keiskei, Wherein the composition is fermented by being inoculated and cultured for 10 to 20 hours. 5. The method of claim 4,
When the lactic acid bacterium is Lactobacillus casei casei, L. plantarum , L. acidophilus , L. bulgaricus , Bifidobacterium longum , Bifidobacterium, Bifidobacterium longum , Bifidobacterium longum , B. bifidum or a mixture thereof. The cosmetic composition for preventing or improving wrinkles of the skin. The present invention relates to a method for preventing or preventing skin wrinkles comprising a mixed extract consisting of Sukjunghwang, Hwanggi, green tea, peony, corn oil, Pueraria, Omija, Angelica gigas, cinnamon, cinnamon and money placenta or an extracted fermented product thereof and honey and a pharmacologically acceptable salt thereof A pharmaceutical composition for improvement. The method according to claim 6,
2 to 10% by weight of steamed rice, 2 to 10% by weight of green tea, 2 to 10% by weight of green tea, 2 to 10% by weight of peanut, 1 to 8% by weight of corn oil, 1 to 8% by weight of Puerariae, 1 to 8% The prevention or improvement of skin wrinkles comprising 0.5 to 5% by weight of celery, 0.5 to 5% by weight of cinnamon, 0.5 to 5% by weight of cinnamon, 0.1 to 2% by weight of carrot, 1 to 3% by weight of placenta and 40 to 60% &Lt; / RTI &gt; The method according to claim 6,
The extract was extracted with distilled water in an amount of 10 times (w / v) of the weight of each of Sukjunggwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica gigas, And recovering the supernatant with filter paper is repeated three times to collect and then dried under reduced pressure to prevent or improve the wrinkle of the skin. The method according to claim 6,
The extracted fermented product is prepared by adding lactic acid bacterium to each extract of Sukjiwha, Hwanggi, Green tea, Peony root, Corn oil, Phellinus linteus, Omija, Angelica keiskei, Wherein the composition is fermented by being inoculated and cultured for 10 to 20 hours. 10. The method of claim 9,
When the lactic acid bacterium is Lactobacillus casei casei, L. plantarum , L. acidophilus , L. bulgaricus , Bifidobacterium longum , Bifidobacterium, Bifidobacterium longum , Bifidobacterium longum , B. bifidum or a mixed microorganism thereof. A method of preventing or preventing skin wrinkles comprising mixed extracts comprising extracts of Sukjwangju, Hwanggi, green tea, peony, corn oil, Puerariae, Omija, Angelica gigas, cinnamon, Food composition for improvement. 12. The method of claim 11,
2 to 10% by weight of steamed rice, 2 to 10% by weight of green tea, 2 to 10% by weight of green tea, 2 to 10% by weight of peanut, 1 to 8% by weight of corn oil, 1 to 8% by weight of Puerariae, 1 to 8% The prevention or improvement of skin wrinkles comprising 0.5 to 5% by weight of celery, 0.5 to 5% by weight of cinnamon, 0.5 to 5% by weight of cinnamon, 0.1 to 2% by weight of carrot, 1 to 3% by weight of placenta and 40 to 60% &Lt; / RTI &gt; 12. The method of claim 11,
The extract was extracted with distilled water in an amount of 10 times (w / v) of the weight of each of Sukjunggwang, Hwanggi, Green tea, Peony, Sansui, Pueraria, Omija, Angelica gigas, And recovering the supernatant with filter paper is repeated three times, followed by drying under reduced pressure to prepare a food composition for preventing or improving skin wrinkles. 12. The method of claim 11,
The extracted fermented product is prepared by adding lactic acid bacterium to each extract of Sukjiwha, Hwanggi, Green tea, Peony root, Corn oil, Phellinus linteus, Omija, Angelica keiskei, Wherein the composition is fermented by being inoculated and cultured for 10 to 20 hours. 15. The method of claim 14,
When the lactic acid bacterium is Lactobacillus casei casei, L. plantarum , L. acidophilus , L. bulgaricus , Bifidobacterium longum , Bifidobacterium, Bifidobacterium longum , Bifidobacterium longum , B. bifidum , or a mixture thereof. &Lt; RTI ID = 0.0 &gt; 18. &lt; / RTI &gt;

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