KR102525129B1 - Composition comprising fermented extracts of unripe plum and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a composition comprising a fermented extract of unripe plum and a preparation method thereof and, more specifically, to a cosmetic composition, a quasi-drug composition, and a food composition for skin whitening, skin trouble alleviation, skin moisturizing, skin wrinkle alleviation, and/or antioxidation, wherein unripe plum fruit pulp with skin and seeds removed is fermented once or twice by using Bacillus subtilis or lactic acid bacteria. The composition of the present invention is obtained by fermenting the unripe plum fruit to provide skin whitening, skin trouble alleviation, an anti-inflammatory effect, moisturizing, skin wrinkle alleviation, and antioxidant activity. Accordingly, the composition can be advantageously utilized as a cosmetic composition, a quasi-drug composition, and/or a food composition for skin care.

Description

Composition containing fermented extract of immature plums and method for preparing the same

The present invention relates to a composition containing a fermented extract of immature plums and a method for producing the same, and more particularly, to a method for preparing the same, and more particularly, to single or double fermentation of the flesh of immature fruits of plums from which skins and seeds have been removed using Bacillus subtilis or lactic acid bacteria. It relates to a cosmetic composition, a quasi-drug composition, and a food composition for skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, and/or antioxidant, characterized by being fermented.

Prunus ( Prunus salicina L. ) is a fruit that has been cultivated since the Shilla Dynasty in Korea, and belongs to the plum subgenus of Prunus miaceae.

Most of the plums produced in Korea are Asian plums, and about 70% are one of the major domestic fruits produced in Gyeongbuk. Plum is a fruit that is harvested early throughout the year, and although the composition varies depending on the variety and environment, it contains a large amount of natural phenolic compounds as useful components.

According to recent studies, it has been found that the phenolic compound is oxidized instead of active oxygen and is effective in antioxidation, anticancer, antibacterial, etc., and is being actively studied.

These plums are mainly consumed raw or processed into dried plums, jams, nectars, juices, wines, and the like. The processed foods of plums as described above mainly use the flesh and skin of plums, and for this, mature fruits, not immature fruits of plums, are required.

However, during the cultivation of plums, a large number of fallen fruits due to abnormal weather occur, and therefore most of the plums are discarded.

Therefore, various attempts have been made to utilize the by-products of these plums, but they are currently insufficient.

On the other hand, there are several factors that determine human skin color. Among them, the activity of melanocytes that produce melanin pigment, the distribution of blood vessels, the thickness of skin, and other genetic factors are important. In particular, the activity of melanocytes in the human body to produce melanin pigment by the action of various enzymes such as tyrosinase is known to be the most important factor.

Melanin is a dark brown pigment produced by oxidative polymerization of phenolic compounds. It not only determines human skin color, but also absorbs ultraviolet rays or exhibits antioxidant effects as a free radical scavenger to protect the skin. It plays an important role in the skin.

However, if the skin is excessively exposed to UV rays, or if melanin is excessively produced due to factors such as hormonal changes or stress, it can cause pigmentation in the skin, making the skin dark and cloudy, or causing spots or freckles, which can negatively affect skin beauty. can

Commonly known whitening ingredients include substances that inhibit tyrosinase enzyme activity such as kojic acid or arbutin, hydroquinone, vitamin C or derivatives thereof, and various plant extracts.

They are known to brighten the skin tone by inhibiting the synthesis of melanin pigment, achieve skin whitening, or improve hyperpigmentation of the skin, such as spots and freckles caused by ultraviolet rays, hormones, or genetic factors, but when applied to the skin, they cause irritation. There was a problem that there was a limit on the amount of use due to safety issues such as overflammation, or a practical effect could not be expected because the effect was insignificant.

Republic of Korea Patent Publication No. 10-2013-0134097

The present invention is to solve the above problems, and as a result of diligent efforts to develop a substance with high utilization value as a cosmetic or quasi-drug for skin whitening without side effects and toxicity to the human body using natural raw materials, the pulp of immature plums By fermenting with Bacillus subtilis and / or lactic acid bacteria, it is intended to provide a composition that is effective not only in skin whitening but also in improving skin troubles, anti-inflammatory, moisturizing, improving skin wrinkles and enhancing antioxidant activity, and a method for producing the composition .

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object can be achieved by a cosmetic composition containing fermented immature plum extract as an active ingredient.

Specifically, the fermented extract of immature plums may be characterized in that immature plums are double fermented using Bacillus subtilis or lactic acid bacteria.

Specifically, the immature plum may be characterized in that the flesh of the immature fruit of the plum from which the skin and seeds are removed.

Specifically, the cosmetic composition may be characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof.

Specifically, the cosmetic composition is a flexible lotion, astringent lotion, nutrient lotion, nutrient cream, massage cream, lotion, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, paste, gel, suspension, emulsion , It may be characterized in that it is formulated with one or more selected from the group consisting of sprays, serums, capsules, and combinations thereof.

Another object of the present invention can be achieved by a quasi-drug composition comprising an extract of fermented immature plums as an active ingredient.

Specifically, the quasi-drug composition may be characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof.

Specifically, the quasi-drug composition is one or more selected from the group consisting of external preparations, powders, sterilization disinfectants, toothpastes, ointments, lotions, internal preparations, wet tissues, sprays, pastes, bands, patches, and combinations thereof. It can be characterized as formulated.

Another object of the present invention can be achieved by a food composition comprising the fermented immature plum extract as an active ingredient.

Another object of the present invention can be achieved by a health functional food comprising the food composition.

Another object of the present invention, (a) preparing an immature plum extract; (b) inoculating the immature plum extract with a fermentation strain and fermenting the immature plum extract at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 for 10 to 20 days to obtain a fermented product; (c) removing the fermentation strain from the fermentation product; And (d) aging the fermented product from which the fermentation strain was removed at a temperature range of 10 ° C to 20 ° C and a humidity range of 70% to 90% for 10 to 30 days to obtain an immature fermented plum fermented extract; comprising , It can be achieved by a method for preparing fermented immature plum extract.

Specifically, the immature plum extract may be characterized in that it is extracted from the pulp of the immature plum fruit from which the skin and seeds are removed.

Specifically, the fermentation strain, Bacillus subtilis ( Bacillus subtilis ) or lactic acid bacteria may be characterized in that.

Another object of the present invention can be achieved by a composition comprising the fermented immature plum extract prepared by the above method.

According to the present invention, by fermenting the pulp of immature fruits of plums using Bacillus subtilis or lactic acid bacteria, it exhibits skin whitening, skin trouble improvement, antioxidant and anti-inflammatory, skin moisturizing, and skin wrinkle improvement effects. Cosmetics for skin care It can be used as a composition, a quasi-drug composition, and/or a food composition.

However, the effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

1 is a graph showing the DPPH free radical scavenging activity of the fermented immature plum extract according to an embodiment of the present invention.
Figure 2 is a graph showing the ABTS free radical scavenging activity of the fermented immature plum extract according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented as examples to explain the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

In addition, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art to which this invention belongs, and in case of conflict, this specification including definitions of will take precedence.

In order to clearly explain the proposed invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification. And, when a certain component is said to "include", this means that it may further include other components without excluding other components unless otherwise stated. Also, a “unit” described in the specification means one unit or block that performs a specific function.

In each step, the identification code (first, second, etc.) is used for convenience of description, and the identification code does not describe the order of each step, and each step does not clearly describe a specific order in context. It may be performed differently from the order specified above. That is, each step may be performed in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order.

Hereinafter, embodiments and embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. However, it may not be limited to these implementations and examples and drawings herein.

One aspect of the present application provides a cosmetic composition comprising an unripe fermented plum extract as an active ingredient.

In one embodiment, the cosmetic composition includes, as an active ingredient, a fermented extract prepared by fermenting immature plums using a fermented strain, thereby improving skin whitening, skin trouble, antioxidant, anti-inflammatory, skin moisturizing, and skin wrinkle improvement. effect can be shown.

In one embodiment, the fermented immature plum extract may include fermented immature plums using a fermentation strain. For example, the fermentation strain may include Bacillus subtilis or lactic acid bacteria, and the fermented extract of the immature plum is inoculated with the immature plum using Bacillus subtilis or lactic acid bacteria, followed by double fermentation. can do.

In the present invention, the term "immature plum" may mean an immature fruit of a plum. More preferably, the immature plums may be characterized in that they are immature fruits and pulp of plums from which skins and seeds are removed.

In one embodiment, the cosmetic composition may be characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof. there is.

In the present invention, the term "lactic acid bacteria" may refer to bacteria that grow by fermentation and produce lactic acid as a main product.

In one embodiment, the lactic acid bacteria may mean Lactobacillus , Streptococcus , Enterococcus , or Pediococcus , and more specifically, Lactobacillus plantarum. plantarum ), but is not limited thereto.

In the present invention, the term "fermentation" may mean any activity or process including enzymatic or metabolic decomposition of organic substances using microorganisms.

In one embodiment, the microorganism used for fermentation is Bacillus , Lactobacillus , Streptococcus , Enterococcus , Bifidobacterium , Pediococcus , Saccharomyces , Schizosaccharomyces , Zygosaccharomyces , Galactomyces , Streptomyces , Candida , Monascus or Aspergillus It may mean bacteria such as Aspergillus , yeast, or fungus.

More specifically, the microorganism may be Bacillus subtilis, that is , Bacillus subtilis or Lactobacilus plantarum , but is not limited thereto.

The "Bacillus subtilis" is a species of rod-shaped bacteria commonly present in nature, such as decaying soil, water, and air, and is a rod-shaped or cylindrical bacterium of various sizes and lengths, and is known to have flagella or spores.

In the present invention, the term "extract" refers to an extract obtained by the extraction of immature plums, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a product thereof. All mixtures are included.

In one embodiment, the extract can be extracted without limitation from natural, hybrid, and genetically modified immature plums, and can also be extracted from tissue culture thereof. Preferably, for the efficiency of extraction, the immature plums may be dried before extraction and then finely pulverized for extraction.

In one embodiment, the fermented extract may be a fermented extract of the immature plum, a fermented product obtained by inoculating the extract of the immature plum with Bacillus subtilis or lactic acid bacteria, and a diluted solution, a concentrate, a dried product thereof, a purified product, or a mixture thereof. may include all of them.

In one embodiment, a method for extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

In one embodiment, the type of extraction solvent used to extract the extract is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water (distilled water); C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; or mixtures thereof may be used. Preferably, water, lower alcohols of C1 to C4, or polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol may be used alone or in combination of two or more for extraction.

In one embodiment, in order to remove floating solid particles and other impurities, the extract is filtered using a method known in the art and then used as it is, or lyophilized, hot air dried, or spray dried. Can be used after drying.

In one embodiment, the fermentation may be carried out at a temperature range of about 20 ° C to about 40 ° C. If the fermentation is performed at less than about 20°C, the fermentation may not be sufficiently performed at an excessively low temperature, and if the temperature exceeds about 40°C, the contents may deteriorate.

In one embodiment, the fermentation may be carried out in a pH range of about 4 to 6. If the fermentation is performed in a range of less than pH 4 or greater than pH 6, fermentation may not proceed sufficiently.

In one embodiment, the fermentation may be performed for about 10 days to about 20 days. If the fermentation is performed for less than about 10 days, fermentation may not be sufficiently performed, and if the fermentation exceeds about 20 days, corruption may occur.

In one embodiment, the fermentation strain may be single or double inoculated. For example, the immature plum may be fermented by inoculating the fermentation strain alone, or two or more fermentation strains may be sequentially double-inoculated and then fermented.

In one embodiment, the fermented immature plum extract may include fermenting immature plums using a fermentation strain, removing the fermentation strain, and aging in a specific temperature and humidity range. By additionally including the aging step, components that have not been fermented can be fermented and the activity of enzymes can be increased. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; It may be obtained by a method including; and obtaining an immature fermented plum fermented extract by aging the fermented product from which the fermentation strain is removed.

In one embodiment, the aging may be performed at a temperature range of about 20 °C to about 40 °C. If the aging is performed at less than about 20 ° C, the effect of the aging may not be sufficiently exerted, and when the temperature exceeds about 40 ° C, the contents may deteriorate.

In one embodiment, the aging may be performed in a humidity range of about 70% to 90%. If the aging is performed in a humidity range of less than about 70%, the effect of the aging may not be sufficiently exerted, and if the humidity exceeds about 90%, the possibility of deterioration of the contents may increase.

In one embodiment, the aging may be performed for about 10 days to about 30 days. If the aging is performed for less than about 10 days, the effect of the aging may not be sufficiently exerted, and if it exceeds about 30 days, decay may occur.

In one embodiment, the fermented immature plum extract may include secondary aging in a crypt after fermentation of immature plums using a fermentation strain and then aging in a specific temperature and humidity range. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; Obtaining an immature fermented plum extract by aging the fermented product from which the fermentation strain is removed; and aging the unripe fermented plum extract in a crypt at a temperature ranging from about 10° C. to about 15° C. for about 30 days to about 90 days. By additionally aging the immature fermented plum extract in a crypt at a low temperature, more excellent antioxidant and anti-inflammatory effects can be exhibited. If the crypt aging is performed at a temperature of less than about 10° C. for less than about 30 days, the antioxidant and anti-inflammatory effects of the crypt aging may not be sufficiently exhibited, and about 90 days at a temperature exceeding about 15° C. If exceeded, the composition may deteriorate.

In one embodiment, the cosmetic composition includes fermented immature plum extract as an active ingredient, and the fermented immature plum extract may be included in an amount of about 0.001 to about 20% by weight based on the total weight of the cosmetic composition. If the fermented plum extract is contained in an amount of less than about 0.001% by weight, the effects of skin whitening, trouble improvement, antioxidant, anti-inflammatory, skin moisturizing and skin wrinkle improvement according to the inclusion of the fermented plum extract may not be sufficiently exhibited. , If it exceeds about 20% by weight, it may cause skin irritation. For example, the unripe fermented plum extract is about 0.001 to about 20% by weight, about 0.001 to about 10% by weight, about 0.001 to about 5% by weight, about 0.001 to about 1% by weight, about 0.001 to about 0.1% by weight, It may be included in about 0.001 to about 0.01% by weight, about 0.01 to about 1% by weight, or about 0.01 to about 0.1% by weight, but is not limited thereto.

In one embodiment, the cosmetic composition may be prepared by further including a blackcurrant juice solution when preparing a fermented immature plum extract. The black currant juice means obtained by cold-pressing the fruit of black currant, also called cassis. Since the black currant juice contains enough polysaccharides, it can be added during the preparation of the fermented extract of the immature plum to further promote fermentation and add a refreshing flavor. For example, the black currant juice may be further included in the step of inoculating the fermentation strain from the immature plum extract to further promote the fermentation.

In one embodiment, the black currant juice may be added in a weight ratio of about 0.1 to 0.3 with respect to the immature plum extract. If the black currant juice is added in a weight ratio of less than about 0.1 of the immature plum extract, the fermentation enhancing effect may not be sufficiently exhibited by adding the black currant juice, and the weight ratio of about 0.3 If it is exceeded, miscibility with other ingredients may be impaired.

In addition to the fermented unripe plum extract, the cosmetic composition of the present invention may further include other components or substances that enhance skin whitening, trouble improvement, anti-inflammatory, moisturizing, skin wrinkle improvement, and antioxidant activity.

In one embodiment, the cosmetic composition may further include sea buckthorn oil. The sea buckthorn oil may exhibit high antioxidant and anti-inflammatory effects by including vitamins, flavonoids, essential amino acids, and fatty acids. Specifically, the sea buckthorn oil contains abundant vitamins such as thiamine, riboflavin, and pyridoxine, vitamin A, vitamin K, and vitamin E, thereby providing vitality and elasticity to the skin, preventing wrinkles and aging, , It exhibits excellent antioxidant efficacy and can impart storage and formulation stability effects when additionally included in a cosmetic composition.

In one embodiment, the cosmetic composition may include about 0.001 to about 0.1% by weight of sea buckthorn oil based on the total weight of the cosmetic composition. If the seabuckthorn oil is included in less than about 0.001% by weight, the anti-inflammatory and antioxidant effects may not be sufficiently exhibited, and if it exceeds about 20% by weight, mixing with other ingredients sexuality may be impaired.

In one embodiment, the cosmetic composition may further include, in addition to the above-described ingredients, components and/or carriers typically required for preparing cosmetic compositions, but may not be limited thereto. For example, water, a surfactant, a humectant, a lower alcohol, a thickener, a chelating agent, a colorant, a preservative, a flavoring agent, and the like may be appropriately blended, but is not limited thereto. In the present invention, the cosmetically acceptable carrier may vary depending on the formulation.

In one embodiment, the cosmetic composition, softening lotion, astringent lotion, nutrient lotion, nutrient cream, massage cream, lotion, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, paste, gel, It may be characterized in that it is formulated with one or more selected from the group consisting of suspensions, emulsions, sprays, serums, capsules, and combinations thereof.

In one embodiment, the formulation of the cosmetic composition may be characterized in that a solution or emulsion formulation.

In one embodiment, when the formulation of the cosmetic composition is a solution or emulsion, the carrier component may include a solvent, a solubilizing agent or an emulsifying agent, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate , benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol, or fatty acid esters of sorbitan, but are not limited thereto.

In one embodiment, when the formulation of the cosmetic composition is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester suspending agents such as, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like, but are not limited thereto.

In one embodiment, the formulation of the cosmetic composition may be characterized in that a paste, cream, or gel formulation.

In one embodiment, when the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc as a carrier component Alternatively, zinc oxide may be included, but is not limited thereto.

In one embodiment, the formulation of the cosmetic composition may be characterized in that the powder or spray formulation.

In one embodiment, when the formulation of the cosmetic composition is a powder or a spray, it may include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in the case of a spray, additionally propellants such as, but not limited to, chlorofluorohydrocarbons, propane/butane or dimethyl ether.

In one embodiment, the formulation of the cosmetic composition may be characterized in that the cleansing formulation.

In one embodiment, when the formulation of the cosmetic composition is surfactant-containing cleansing, carrier components include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, Sarcosinates, fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be included, but are not limited thereto. The carrier components included in the cosmetic composition of the present invention may be appropriately included in an amount commonly used in the art.

In one embodiment, the cosmetic composition includes commonly used adjuvants, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic actives, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, in addition to the immature fermented plum extract. , absorbents and dyes, and the like.

In the present invention, the term "skin whitening" refers to not only brightening the skin tone by inhibiting the synthesis of melanin pigment, but also improving skin hyperpigmentation such as spots or freckles caused by ultraviolet rays, hormones, stress, or genetic factors. it means.

In the present invention, the term "skin trouble" means a skin disease or symptom that can be caused by inflammation caused by excessive production of nitric oxide (NO) in macrophages. The skin trouble includes all skin diseases related to inflammation regardless of their type, and non-limiting examples of skin diseases or symptoms related to the inflammation include skin rash, acne, pimple, atopic dermatitis, psoriasis, erythematous disease, skin pruritus, seborrheic eczema, etc., but are not limited thereto.

In the present invention, the term "trouble improvement" means suppressing or inhibiting the generation of the trouble on the skin, or alleviating the trouble that has already been created, and more specifically, suppressing the production of inflammation on the skin or inflammation that has already been created. It may contain "anti-inflammatory" activity that alleviates and improves

In the present invention, the term “moisturizing the skin” may mean increasing the feeling of moisture in the skin and maintaining a moist state.

In the present invention, the term "wrinkle improvement" means suppressing or inhibiting the formation of wrinkles on the skin, or relieving wrinkles already formed, a state in which elastin and collagen sufficiently exist in the skin layer. Maintaining and improving skin elasticity may also be included in wrinkle improvement of the present invention.

In the present invention, the term "antioxidation" refers to the oxidation of cells by free radicals or reactive oxygen species (ROS) generated by oxidative stress caused by intracellular metabolism or ultraviolet light. It means to inhibit, and may include reducing damage to cells caused by removing free radicals or reactive oxygen species.

In one embodiment, as specifically confirmed through the examples, the cosmetic composition of the present invention has a very good free radical (DPPH, ABTS) scavenging ability, and is effective in skin antioxidant, whitening, skin trouble improvement, moisturizing, and wrinkle improvement. It can be seen that there is efficacy.

Another aspect of the present application provides a quasi-drug composition comprising the fermented immature plum extract as an active ingredient.

In one embodiment, the fermented immature plum extract may include fermented immature plums using a fermentation strain. For example, the fermentation strain may include Bacillus subtilis or lactic acid bacteria, and the fermented extract of the immature plum is inoculated with the immature plum using Bacillus subtilis or lactic acid bacteria, followed by double fermentation. can do.

In one embodiment, the immature plum may be characterized in that the immature plum and the pulp of the plum from which the skin and seeds are removed.

In one embodiment, the lactic acid bacteria may mean Lactobacillus , Streptococcus , Enterococcus , or Pediococcus , and more specifically, Lactobacillus plantarum. plantarum ), but is not limited thereto.

In one embodiment, the microorganism used for fermentation is Bacillus , Lactobacillus , Streptococcus , Enterococcus , Bifidobacterium , Pediococcus , Saccharomyces , Schizosaccharomyces , Zygosaccharomyces , Galactomyces , Streptomyces , Candida , Monascus or Aspergillus It may mean bacteria such as Aspergillus , yeast, or fungus.

More specifically, the microorganism may be Bacillus subtilis, that is , Bacillus subtilis or Lactobacilus plantarum , but is not limited thereto.

In one embodiment, the fermentation may be carried out at a temperature range of about 20 ° C to about 40 ° C. If the fermentation is performed at less than about 20°C, the fermentation may not be sufficiently performed at an excessively low temperature, and if the temperature exceeds about 40°C, the contents may deteriorate.

In one embodiment, the fermentation may be carried out in a pH range of about 4 to 6. If the fermentation is performed in a range of less than pH 4 or greater than pH 6, fermentation may not proceed sufficiently.

In one embodiment, the fermentation may be performed for about 10 days to about 20 days. If the fermentation is performed for less than about 10 days, fermentation may not be sufficiently performed, and if the fermentation exceeds about 20 days, corruption may occur.

In one embodiment, the fermentation strain may be single or double inoculated. For example, the immature plum may be fermented by inoculating the fermentation strain alone, or two or more fermentation strains may be sequentially double-inoculated and then fermented.

In one embodiment, the fermented immature plum extract may include fermenting immature plums using a fermentation strain, removing the fermentation strain, and aging in a specific temperature and humidity range. By additionally including the aging step, components that have not been fermented can be fermented and the activity of enzymes can be increased. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; It may be obtained by a method including; and obtaining an immature fermented plum fermented extract by aging the fermented product from which the fermentation strain is removed.

In one embodiment, the aging may be performed at a temperature range of about 20 °C to about 40 °C. If the aging is performed at less than about 20 ° C, the effect of the aging may not be sufficiently exerted, and when the temperature exceeds about 40 ° C, the contents may deteriorate.

In one embodiment, the aging may be performed in a humidity range of about 70% to 90%. If the aging is performed in a humidity range of less than about 70%, the effect of the aging may not be sufficiently exerted, and if the humidity exceeds about 90%, the possibility of deterioration of the contents may increase.

In one embodiment, the aging may be performed for about 10 days to about 30 days. If the aging is performed for less than about 10 days, the effect of the aging may not be sufficiently exerted, and if it exceeds about 30 days, decay may occur.

In one embodiment, the fermented immature plum extract may include secondary aging in a crypt after fermentation of immature plums using a fermentation strain and then aging in a specific temperature and humidity range. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; Obtaining an immature fermented plum extract by aging the fermented product from which the fermentation strain is removed; and aging the unripe fermented plum extract in a crypt at a temperature ranging from about 10° C. to about 15° C. for about 30 days to about 90 days. By additionally aging the immature fermented plum extract in a crypt at a low temperature, more excellent antioxidant and anti-inflammatory effects can be exhibited. If the crypt aging is performed at a temperature of less than about 10° C. for less than about 30 days, the antioxidant and anti-inflammatory effects of the crypt aging may not be sufficiently exhibited, and about 90 days at a temperature exceeding about 15° C. If exceeded, the composition may deteriorate.

In one embodiment, the quasi-drug composition may be prepared by further including black currant juice when preparing the fermented extract of unripe plums. The black currant juice means obtained by cold-pressing the fruit of black currant, also called cassis. Since the black currant juice contains enough polysaccharides, it can be added during the preparation of the fermented extract of the immature plum to further promote fermentation and add a refreshing flavor. For example, the black currant juice may be further included in the step of inoculating the fermentation strain from the immature plum extract to further promote the fermentation.

In one embodiment, the black currant juice may be added in a weight ratio of about 0.1 to 0.3 with respect to the immature plum extract. If the black currant juice is added in a weight ratio of less than about 0.1 of the immature plum extract, the fermentation enhancing effect may not be sufficiently exhibited by adding the black currant juice, and the weight ratio of about 0.3 If it is exceeded, miscibility with other ingredients may be impaired.

In one embodiment, the quasi-drug composition may further include sea buckthorn oil. The sea buckthorn oil may exhibit high antioxidant and anti-inflammatory effects by including vitamins, flavonoids, essential amino acids, and fatty acids. Specifically, the sea buckthorn oil contains abundant vitamins such as thiamine, riboflavin, and pyridoxine, vitamin A, vitamin K, and vitamin E, thereby providing vitality and elasticity to the skin, preventing wrinkles and aging, , It exhibits excellent antioxidant efficacy, and when added to a quasi-drug composition, storage and formulation stability effects can be imparted.

In one embodiment, the quasi-drug composition may contain seabuckthorn oil in an amount of about 0.001 to about 0.1% by weight based on the total weight of the quasi-drug composition. If the seabuckthorn oil is included in less than about 0.001% by weight, the anti-inflammatory and antioxidant effects may not be sufficiently exhibited, and if it exceeds about 20% by weight, mixing with other ingredients sexuality may be impaired.

In one embodiment, the quasi-drug composition may be characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof there is.

In one embodiment, the quasi-drug composition is selected from the group consisting of external preparations, powders, sterilization disinfectants, dentifrices, ointments, lotions, internal preparations, wet tissues, sprays, pastes, bands, patches, and combinations thereof It may be characterized in that it is formulated with one or more.

In one embodiment, the quasi-drug composition may further include a pharmaceutically acceptable carrier, excipient, or diluent, if necessary, in addition to the fermented immature plum extract. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as it can achieve the effect of the present invention, for example, a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, a lubricant, a sweetener, a flavoring agent, and a preservative. etc. may be included.

In one embodiment, representative examples of the pharmaceutically acceptable carrier, excipient, or diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia gum, alginate, Calcium phosphate, calcium carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycols, vegetable oils, injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like, but are not limited thereto.

In one embodiment, when the composition of the present invention is used as a quasi-drug, it may further contain at least one active ingredient having the same or similar function as the fermented immature plum extract. For example, it may further include a component having known skin whitening, trouble improvement, anti-inflammatory, moisturizing, skin wrinkle improvement or antioxidant activity, in this case, the composition of the present invention for skin whitening, trouble improvement, anti-inflammatory, moisturizing , skin wrinkle improvement and antioxidant activity can be further increased. When adding the active ingredient, skin safety according to combined use, ease of formulation, and stability of the active ingredient may be considered. The additional active ingredient may be included in an amount of about 0.0001 to about 10% by weight (w/w) based on the total weight of the quasi-drug composition, and the content range may be appropriately adjusted by a person skilled in the art in consideration of skin safety, ease of formulation, etc. there is.

In one embodiment, the quasi-drug composition may further include other components or substances that enhance skin whitening, trouble improvement, anti-inflammatory, moisturizing, skin wrinkle improvement, and antioxidant activity, in addition to the fermented unripe plum extract.

In one embodiment, the quasi-drug composition is an external preparation, powder, disinfectant, toothpaste, ointment, lotion, internal preparation (vitamin/mineral preparation, nutritional tonic), wet tissue, spray paste, band or patch ), etc., but is not particularly limited thereto, and the formulation method, dosage, method of use, components, etc. of quasi-drugs can be appropriately selected by those skilled in the art from conventional techniques known in the art.

In one embodiment, the quasi-drug composition includes an extract of fermented unripe plums as an active ingredient, and the fermented extract of unripe plums may be included in an amount of about 0.001 to about 20% by weight based on the total weight of the quasi-drug composition. If the fermented plum extract is contained in an amount of less than about 0.001% by weight, the effects of skin whitening, trouble improvement, antioxidant, anti-inflammatory, skin moisturizing and skin wrinkle improvement according to the inclusion of the fermented plum extract may not be sufficiently exhibited. , If it exceeds about 20% by weight, it may cause skin irritation. For example, the unripe fermented plum extract is about 0.001 to about 20% by weight, about 0.001 to about 10% by weight, about 0.001 to about 5% by weight, about 0.001 to about 1% by weight, about 0.001 to about 0.1% by weight, It may be included in about 0.001 to about 0.01% by weight, about 0.01 to about 1% by weight, or about 0.01 to about 0.1% by weight, but is not limited thereto.

Another aspect of the present invention provides a food composition comprising the fermented immature plum extract as an active ingredient.

In one embodiment, the fermented immature plum extract may include fermented immature plums using a fermentation strain. For example, the fermentation strain may include Bacillus subtilis or lactic acid bacteria, and the fermented extract of the immature plum is inoculated with the immature plum using Bacillus subtilis or lactic acid bacteria, followed by double fermentation. can do.

In one embodiment, the immature plum may be characterized in that the immature plum and the pulp of the plum from which the skin and seeds are removed.

In one embodiment, the lactic acid bacteria may mean Lactobacillus , Streptococcus , Enterococcus , or Pediococcus , and more specifically, Lactobacillus plantarum. plantarum ), but is not limited thereto.

In one embodiment, the microorganism used for fermentation is Bacillus , Lactobacillus , Streptococcus , Enterococcus , Bifidobacterium , Pediococcus , Saccharomyces , Schizosaccharomyces , Zygosaccharomyces , Galactomyces , Streptomyces , Candida , Monascus or Aspergillus It may mean bacteria such as Aspergillus , yeast, or fungus.

More specifically, the microorganism may be Bacillus subtilis, that is , Bacillus subtilis or Lactobacilus plantarum , but is not limited thereto.

In one embodiment, the fermentation may be carried out at a temperature range of about 20 ° C to about 40 ° C. If the fermentation is performed at less than about 20°C, the fermentation may not be sufficiently performed at an excessively low temperature, and if the temperature exceeds about 40°C, the contents may deteriorate.

In one embodiment, the fermentation may be carried out in a pH range of about 4 to 6. If the fermentation is performed in a range of less than pH 4 or greater than pH 6, fermentation may not proceed sufficiently.

In one embodiment, the fermentation may be performed for about 10 days to about 20 days. If the fermentation is performed for less than about 10 days, fermentation may not be sufficiently performed, and if the fermentation exceeds about 20 days, corruption may occur.

In one embodiment, the fermentation strain may be single or double inoculated. For example, the immature plum may be fermented by inoculating the fermentation strain alone, or two or more fermentation strains may be sequentially double-inoculated and then fermented.

In one embodiment, the fermented immature plum extract may include fermenting immature plums using a fermentation strain, removing the fermentation strain, and aging in a specific temperature and humidity range. By additionally including the aging step, components that have not been fermented can be fermented and the activity of enzymes can be increased. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; It may be obtained by a method including; and obtaining an immature fermented plum fermented extract by aging the fermented product from which the fermentation strain is removed.

In one embodiment, the aging may be performed at a temperature range of about 20 °C to about 40 °C. If the aging is performed at less than about 20 ° C, the effect of the aging may not be sufficiently exerted, and when the temperature exceeds about 40 ° C, the contents may deteriorate.

In one embodiment, the aging may be performed in a humidity range of about 70% to 90%. If the aging is performed in a humidity range of less than about 70%, the effect of the aging may not be sufficiently exerted, and if the humidity exceeds about 90%, the possibility of deterioration of the contents may increase.

In one embodiment, the aging may be performed for about 10 days to about 30 days. If the aging is performed for less than about 10 days, the effect of the aging may not be sufficiently exerted, and if it exceeds about 30 days, decay may occur.

In one embodiment, the fermented immature plum extract may include secondary aging in a crypt after fermentation of immature plums using a fermentation strain and then aging in a specific temperature and humidity range. For example, the fermented extract of immature plums may include preparing an extract of immature plums from immature plums; fermenting by inoculating the fermentation strain into the immature plum extract; Preparing a fermentation strain from the fermented product produced by the fermentation; Obtaining an immature fermented plum extract by aging the fermented product from which the fermentation strain is removed; and aging the unripe fermented plum extract in a crypt at a temperature ranging from about 10° C. to about 15° C. for about 30 days to about 90 days. By additionally aging the immature fermented plum extract in a crypt at a low temperature, more excellent antioxidant and anti-inflammatory effects can be exhibited. If the crypt aging is performed at a temperature of less than about 10° C. for less than about 30 days, the antioxidant and anti-inflammatory effects of the crypt aging may not be sufficiently exhibited, and about 90 days at a temperature exceeding about 15° C. If exceeded, the composition may deteriorate.

In one embodiment, the food composition may be prepared by further including black currant juice when preparing the fermented immature plum extract. The black currant juice means obtained by cold-pressing the fruit of black currant, also called cassis. Since the black currant juice contains enough polysaccharides, it can be added during the preparation of the fermented extract of the immature plum to further promote fermentation and add a refreshing flavor. For example, the black currant juice may be further included in the step of inoculating the fermentation strain from the immature plum extract to further promote the fermentation.

In one embodiment, the black currant juice may be added in a weight ratio of about 0.1 to 0.3 with respect to the immature plum extract. If the black currant juice is added in a weight ratio of less than about 0.1 of the immature plum extract, the fermentation enhancing effect may not be sufficiently exhibited by adding the black currant juice, and the weight ratio of about 0.3 If it is exceeded, miscibility with other ingredients may be impaired.

In one embodiment, the food composition may include about 0.001 to about 20% by weight of the fermented immature plum extract based on the total weight of the food composition. If the fermented plum extract is less than about 0.001% by weight, the effect of including the fermented plum extract may not be sufficiently exhibited, and if it exceeds about 20% by weight, skin irritation may occur. For example, the unripe fermented plum extract is about 0.001 to about 20% by weight, about 0.001 to about 10% by weight, about 0.001 to about 5% by weight, about 0.001 to about 1% by weight, about 0.001 to about 0.1% by weight, It may be included in about 0.001 to about 0.01% by weight, about 0.01 to about 1% by weight, or about 0.01 to about 0.1% by weight, but is not limited thereto.

In one embodiment, the food composition may further include other components or substances that enhance skin whitening, trouble improvement, anti-inflammation, moisturizing, skin wrinkle improvement, and antioxidant activity in addition to the fermented unripe plum extract.

In one embodiment, the food composition may further include sea buckthorn oil. The sea buckthorn oil may exhibit high antioxidant and anti-inflammatory effects by including vitamins, flavonoids, essential amino acids, and fatty acids. Specifically, the sea buckthorn oil contains abundant vitamins such as thiamine, riboflavin, and pyridoxine, vitamin A, vitamin K, and vitamin E, thereby providing vitality and elasticity to the skin, preventing wrinkles and aging, , It exhibits excellent antioxidant efficacy and can impart storage and formulation stability effects when added to food compositions.

In one embodiment, the food composition may include about 0.001 to about 0.1% by weight of sea buckthorn oil based on the total weight of the food composition. If the seabuckthorn oil is included in less than about 0.001% by weight, the anti-inflammatory and antioxidant effects may not be sufficiently exhibited, and if it exceeds about 20% by weight, mixing with other ingredients sexuality may be impaired.

In one embodiment, the food composition may include a food chemically acceptable food supplement additive in addition to the fermented immature plum extract. The term "food supplement additive" refers to a material that can be supplementally added to food, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food supplement additives include nutrients, vitamins, electrolytes, synthetic or natural flavors, colorants, fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, Carbonation agents used in alcohol and carbonated beverages, etc. are included, but are not limited thereto.

In one embodiment, the food composition includes the fermented unripe plum extract as an active ingredient, and the fermented unripe plum extract may be included in about 0.001 to about 20% by weight based on the total weight of the food composition. If the fermented plum extract is less than about 0.001% by weight, the effect of including the fermented plum extract may not be sufficiently exhibited, and if it exceeds about 20% by weight, irritation may occur. For example, the unripe fermented plum extract is about 0.001 to about 20% by weight, about 0.001 to about 10% by weight, about 0.001 to about 5% by weight, about 0.001 to about 1% by weight, about 0.001 to about 0.1% by weight, It may be included in about 0.001 to about 0.01% by weight, or about 0.01 to about 1% by weight, but is not limited thereto.

Another aspect of the present invention provides a health functional food comprising the food composition.

In the present invention, the term "health functional food" refers to food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, "functionality" means obtaining useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation. In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of using food as a raw material and not having side effects that can occur when taking drugs for a long time, and is effective in skin whitening, trouble improvement, anti-inflammatory , It can be taken as an adjuvant to moisturize, improve skin wrinkles, and enhance antioxidant activity.

There is no limit to the form that the health functional food of the present invention can take, and it may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as health functional food and functional food. In addition, the health functional food of the present invention can be prepared by mixing suitable other auxiliary ingredients and known additives that can be included in food according to the selection of those skilled in the art. Examples of the health functional food include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and vitamins There are complex agents, etc., and it can be prepared by adding juice, tea, jelly, juice, etc. prepared using the composition according to the present invention as a main component.

In one embodiment, the health functional food may be characterized in that inner beauty food.

In the present invention, the term "inner beauty food" is also called cosmetic or beauty food to eat, and refers to food that improves skin constitution healthily by absorbing ingredients good for skin beauty and health into the body. Just like choosing cosmetics for your type, you can select and consume “inner beauty” foods that are suitable for you by considering your skin condition and lifestyle. The health functional food of the present invention can exhibit more excellent skin whitening, trouble improvement, anti-inflammation, moisturizing, skin wrinkle improvement and antioxidant activity by being consumed in the form of inner beauty food.

Another aspect of the present invention, (a) preparing an immature plum extract; (b) inoculating the immature plum extract with a fermentation strain and fermenting the immature plum extract at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 for 10 to 20 days to obtain a fermented product; (c) removing the fermentation strain from the fermentation product; And (d) aging the fermented product from which the fermentation strain was removed at a temperature range of 10 ° C to 20 ° C and a humidity range of 70% to 90% for 10 to 30 days to obtain an immature fermented plum fermented extract; comprising , It provides a method for producing an immature fermented plum extract.

In one embodiment, the immature plum extract may be characterized in that it is extracted from immature plums and pulp from which the skin and seeds are removed.

In one embodiment, the fermentation strain may include Bacillus subtilis or lactic acid bacteria, and the fermented extract of immature plums is obtained by inoculating the immature plums with Bacillus subtilis or lactic acid bacteria and then double fermenting. can be characterized.

In one embodiment, the lactic acid bacteria may mean Lactobacillus , Streptococcus , Enterococcus , or Pediococcus , and more specifically, Lactobacillus plantarum. plantarum ), but is not limited thereto.

In one embodiment, the microorganism used for fermentation is Bacillus , Lactobacillus , Streptococcus , Enterococcus , Bifidobacterium , Pediococcus , Saccharomyces , Schizosaccharomyces , Zygosaccharomyces , Galactomyces , Streptomyces , Candida , Monascus or Aspergillus It may mean bacteria such as Aspergillus , yeast, or fungus.

More specifically, the microorganism may be Bacillus subtilis, that is , Bacillus subtilis or Lactobacilus plantarum , but is not limited thereto.

In one embodiment, the fermentation may be carried out at a temperature range of about 20 ° C to about 40 ° C. If the fermentation is performed at less than about 20°C, the fermentation may not be sufficiently performed at an excessively low temperature, and if the temperature exceeds about 40°C, the contents may deteriorate.

In one embodiment, the fermentation may be carried out in a pH range of about 4 to 6. If the fermentation is performed in a range of less than pH 4 or greater than pH 6, fermentation may not proceed sufficiently.

In one embodiment, the fermentation may be performed for about 10 days to about 20 days. If the fermentation is performed for less than about 10 days, fermentation may not be sufficiently performed, and if the fermentation exceeds about 20 days, corruption may occur.

In one embodiment, the fermentation strain may be single or double inoculated. For example, the immature plum may be fermented by inoculating the fermentation strain alone, or two or more fermentation strains may be sequentially double-inoculated and then fermented.

In one embodiment, it may include fermenting immature plums using a fermentation strain, removing the fermentation strain, and aging in a specific temperature and humidity range. By including the aging step, components that have not yet been fermented can be fermented and the activity of enzymes can be increased.

In one embodiment, the aging may be performed at a temperature range of about 20 °C to about 40 °C. If the aging is performed at less than about 20 ° C, the effect of the aging may not be sufficiently exerted, and when the temperature exceeds about 40 ° C, the contents may deteriorate.

In one embodiment, the aging may be performed in a humidity range of about 70% to 90%. If the aging is performed in a humidity range of less than about 70%, the effect of the aging may not be sufficiently exerted, and if the humidity exceeds about 90%, the possibility of deterioration of the contents may increase.

In one embodiment, the aging may be performed for about 10 days to about 30 days. If the aging is performed for less than about 10 days, the effect of the aging may not be sufficiently exerted, and if it exceeds about 30 days, decay may occur.

In one embodiment, after the step of obtaining the fermented immature plum extract by aging the fermented product from which the fermentation strain is removed, a step of crypt-aging the fermented immature plum extract may be further included. For example, after the step of obtaining an immature fermented plum fermented extract by aging the fermented product from which the fermentation strain was removed at a temperature range of 10 ° C to 20 ° C and a humidity range of 70% to 90% for 10 to 30 days, the A step of maturing the unripe fermented plum extract in the crypt at a temperature range of about 10 ° C to 15 ° C for about 30 days to about 90 days may be further included. By additionally aging the immature fermented plum extract in a crypt at a low temperature, more excellent antioxidant and anti-inflammatory effects can be exhibited. If the crypt aging is performed at a temperature of less than about 10° C. for less than about 30 days, the antioxidant and anti-inflammatory effects of the crypt aging may not be sufficiently exhibited, and about 90 days at a temperature exceeding about 15° C. If exceeded, the composition may deteriorate.

Another aspect of the present invention provides a composition comprising the fermented immature plum extract prepared by the above method. Such a composition is a composition for antioxidant, whitening, skin trouble improvement, moisturizing, wrinkle improvement, etc., and includes, for example, a cosmetic composition, a food composition, a quasi-drug composition, and the like.

In one embodiment, the composition containing the fermented immature plum extract as an active ingredient can be used for skin whitening, trouble improvement, skin moisturizing and / or wrinkle improvement methods, including the step of applying it to the skin of a subject, The subject includes without limitation mammals including mice, livestock, humans, etc., and may preferably mean humans.

In one embodiment, the composition may be prepared by further including black currant juice when preparing the fermented immature plum extract. The black currant juice means obtained by cold-pressing the fruit of black currant, also called cassis. Since the black currant juice contains enough polysaccharides, it can be added during the preparation of the fermented extract of the immature plum to further promote fermentation and add a refreshing flavor. For example, the black currant juice may be further included in the step of inoculating the fermentation strain from the immature plum extract to further promote the fermentation.

In one embodiment, the black currant juice may be added in a weight ratio of about 0.1 to 0.3 with respect to the immature plum extract. If the black currant juice is added in a weight ratio of less than about 0.1 of the immature plum extract, the fermentation enhancing effect may not be sufficiently exhibited by adding the black currant juice, and the weight ratio of about 0.3 If it is exceeded, miscibility with other ingredients may be impaired.

In one embodiment, the composition may further include sea buckthorn oil. The sea buckthorn oil may exhibit high antioxidant and anti-inflammatory effects by including vitamins, flavonoids, essential amino acids, and fatty acids. Specifically, the sea buckthorn oil contains abundant vitamins such as thiamine, riboflavin, and pyridoxine, vitamin A, vitamin K, and vitamin E, thereby providing vitality and elasticity to the skin, preventing wrinkles and aging, , It exhibits excellent antioxidant efficacy and can impart storage and formulation stability effects when added to food compositions.

In one embodiment, the composition may include about 0.001 to about 0.1% by weight of sea buckthorn oil based on the total weight of the composition. If the seabuckthorn oil is included in less than about 0.001% by weight, the anti-inflammatory and antioxidant effects may not be sufficiently exhibited, and if it exceeds about 20% by weight, mixing with other ingredients sexuality may be impaired.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

[Production Example 1]

First, the pulp of immature plums from which skins and seeds have been removed is washed and dried, and then the dried samples are pulverized into 3 L of water (distilled water) or 60% (v/v) ethanol, respectively, and cooled with a cooling condenser. Extraction was performed by boiling for 3 hours in a reflux extractor. Thereafter, it was filtered with a filter cloth to remove insoluble substances and impurities, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and then freeze-dried to prepare an immature plum extract.

Next, after high-pressure sterilization of the prepared immature plum extract, Bacillus subtilis, Lactobacilus plantarum, and Bacillus subtilis and Lactobacillus plantarum were inoculated as fermentation strains, respectively, to prepare the fermented extract. (Bacillus subtilis fermentation group, lactic acid bacteria fermentation group, and double fermentation group). The immature plum extract was fermented for 14 days in a fermentor at 30 ° C and pH 5, and the prepared fermentation broth was vacuum sterilized at 85 ° C and centrifuged to first remove the fermentation strain. Thereafter, the fermented product filtered with filter paper was aged for 20 days at 15° C. and 80% humidity conditions to finally prepare a Bacillus subtilis fermentation extract, a lactic acid bacteria fermentation extract, and a double fermentation extract of immature plums.

[Production Example 2]

First, the pulp of immature plums from which skins and seeds have been removed is washed and dried, and then the dried samples are pulverized into 3 L of water (distilled water) or 60% (v/v) ethanol, respectively, and cooled with a cooling condenser. Extraction was performed by boiling for 3 hours in a reflux extractor. Thereafter, it was filtered with a filter cloth to remove insoluble substances and impurities, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and then freeze-dried to prepare an immature plum extract.

Next, after high-pressure sterilization of the prepared immature plum extract, 0.25 weight ratio of black currant juice was added and mixed with respect to the total 100 parts by weight of the immature plum extract. Thereafter, Bacillus subtilis and Lactobacillus plantarum were double inoculated as fermentation strains to prepare the fermented extract. The immature plum extract was fermented in a fermentor at 30 ° C. and pH 5 for 14 days, and the prepared fermentation broth was vacuum sterilized at 85 ° C. and then centrifuged to first remove the fermented strain. Thereafter, the fermented product filtered through filter paper was aged for 20 days at 15° C. and 80% humidity conditions, and secondaryly aged for 45 days in a crypt at 10° C. to finally prepare a double fermentation extract of immature plums.

[Experimental Example 1: Confirmation of free radical scavenging activity]

The free radical scavenging activity was measured using DPPH and ABTS of the fermented Bacillus subtilis extract, the fermented lactic acid bacteria extract, and the double fermented extract of immature plums prepared in Preparation Example 1.

Specifically, after preparing a standard DPPH ethanol solution of 1.5 mM (0.06 mg / ml), 50 mg of ethanol was added to the Bacillus subtilis fermented extract, lactic acid bacteria fermented extract, and double fermented extract of immature plums prepared in Preparation Example 1, respectively. Samples were made at a concentration of /mL.

To the control, ascorbic acid (antioxidant), ethanol was added to prepare a sample at a concentration of 1 μg/mL. Thereafter, each sample and the standard DPPH solution were added in the same ratio, stirred well, reacted at 37° C. for 30 minutes, and absorbance was measured at 520 nm.

In addition, after preparing an ABTS ethanol solution in the same manner as above, ethanol was added to each of the fermented extract of Bacillus subtilis immature plum, the fermented lactic acid bacteria extract, and the double fermentation extract of Preparation Example 1 to prepare samples at a concentration of 5 mg/mL. To the control, ascorbic acid (antioxidant), ethanol was added to make a sample at a concentration of 1 μg/mL. Thereafter, each sample and standard ABTS solution were added in the same ratio, stirred well, reacted at 37° C. for 30 minutes, and absorbance was measured at 520 nm.

As a result, as shown in FIGS. 1 and 2, the free radical (DPPH, ABTS) scavenging ability of the fermented extract of the immature plum, the fermented lactic acid bacteria extract, and the double fermented extract all increased as the concentration increased. Compared to corbic acid, it showed significantly superior free radical scavenging activity, and it was confirmed that it was effective in antioxidation, whitening, skin trouble, moisturizing, and wrinkle improvement.

[Example 1]

The composition of Example 1 was prepared by mixing the double fermented extract of immature plums prepared in Preparation Example 1 with the components shown in Table 1 below.

Ingredients Example 1 Water 66.82025952 1,2-Hexanediol 1.71439500 Immature Plum Double Fermented Extract 0.01371516 Immature Plum Double Fermented Extract 0.01371516 Immature Plum Double Fermented Extract 0.01371516 Butylene Glycol 12.00000000 Glycerin 4.97500000 Water 0.02500000 Propanediol 5.00000000 Water 4.55000000 Butylene Glycol 0.25000000 1,2-Hexanediol 0.15000000 Hevea Brasiliensis Leaf Extract 0.05000000 Niacinamide 2.00000000 Water 0.52000000 Glycerin 0.43000000 Glyceryl Acrylate/Acrylic Acid Copolymer 0.02000000 1,2-Hexanediol 0.02000000 PVM/MA Copolymer 0.01000000 Water 0.48500000 1,2-Hexanediol 0.01000000 Sodium Hyaluronate 0.00500000 1,2-Hexanediol 0.30000000 Polyglyceryl-10 Laurate 0.20000000 Gluconolactone 0.12000000 Dipropylene Glycol 0.05490000 Hydroxyacetophenone 0.03000000 Caprylyl Glycol 0.01500000 Dipotassium Glycyrrhizate 0.00010000 Water 0.02890000 Butylene Glycol 0.01000000 Diospyros Kaki Leaf Extract 0.01000000 Vitis Vinifera (Grape) Fruit Extract 0.01000000 Coffea Arabica (Coffee) Seed Extract 0.00700000 Carthamus Tinctorius (Safflower) Flower Extract 0.00700000 Polygonum Cuspidatum Root Extract 0.00700000 Camellia Sinensis Leaf Extract 0.00600000 Castanea Crenata (Chestnut) Shell Extract 0.00600000 Zanthoxylum Piperitum Fruit Extract 0.00600000 1,2-Hexanediol 0.00200000 Ethylhexylglycerin 0.00010000 Adenosine 0.04000000 Ammonium Acryloyldimethyltaurate/VP Copolymer 0.03000000 Sodium Phytate 0.01000000 Betaine 0.01000000 Allantoin 0.01000000 Succinic Acid 0.00100000 Panthenol 0.00100000 Pentylene Glycol 0.00100000 Water 0.00068490 Caprylic/Capric Triglycerides 0.00010000 Glycerin 0.00010000 Glyceryl Citrate/Lactate/Linoleate/Oleate 0.00002000 Cetyl Ethylhexanoate 0.00002000 1,2-Hexanediol 0.00002000 Butylene Glycol 0.00002000 Taraxacum Officinale (Dandelion) Leaf Extract 0.00001000 Prunus Persica (Peach) Flower Extract 0.00001000 Polyglyceryl-10 Myristate 0.00001000 Cholesterol 0.00000200 Ethyl Linoleate 0.00000100 Ceramide NP 0.00000100 Lactobacillus Ferment Lysate 0.00000100 Palmitic Acid 0.000000055 Stearic Acid 0.000000045 Water 0.00008750 Butylene Glycol 0.00001000 1,2-Hexanediol 0.00000200 Sodium Guaiazulene Sulfonate 0.00000050 Ceramide NP 0.00010000 Sum 100.00000000

[Example 2 and Example 3]

Compositions of Examples 2 and 3 were prepared by mixing the double fermented extract of immature plums prepared in Preparation Example 2 with the components shown in Table 2 below.

Ingredients Example 2 Example 3 Water 66.82025952 66.81525952 1,2-Hexanediol 1.71439500 1.71439500 Immature Plum Double Fermented Extract 0.01371516 0.01371516 Immature Plum Double Fermented Extract 0.01371516 0.01371516 Immature Plum Double Fermented Extract 0.01371516 0.01371516 Seabuckthorn Oil 0.00000000 0.00500000 Butylene Glycol 12.00000000 12.00000000 Glycerin 4.97500000 4.97500000 Water 0.02500000 0.02500000 Propanediol 5.00000000 5.00000000 Water 4.55000000 4.55000000 Butylene Glycol 0.25000000 0.25000000 1,2-Hexanediol 0.15000000 0.15000000 Hevea Brasiliensis Leaf Extract 0.05000000 0.05000000 Niacinamide 2.00000000 2.00000000 Water 0.52000000 0.52000000 Glycerin 0.43000000 0.43000000 Glyceryl Acrylate/Acrylic Acid Copolymer 0.02000000 0.02000000 1,2-Hexanediol 0.02000000 0.02000000 PVM/MA Copolymer 0.01000000 0.01000000 Water 0.48500000 0.48500000 1,2-Hexanediol 0.01000000 0.01000000 Sodium Hyaluronate 0.00500000 0.00500000 1,2-Hexanediol 0.30000000 0.30000000 Polyglyceryl-10 Laurate 0.20000000 0.20000000 Gluconolactone 0.12000000 0.12000000 Dipropylene Glycol 0.05490000 0.05490000 Hydroxyacetophenone 0.03000000 0.03000000 Caprylyl Glycol 0.01500000 0.01500000 Dipotassium Glycyrrhizate 0.00010000 0.00010000 Water 0.02890000 0.02890000 Butylene Glycol 0.01000000 0.01000000 Diospyros Kaki Leaf Extract 0.01000000 0.01000000 Vitis Vinifera (Grape) Fruit Extract 0.01000000 0.01000000 Coffea Arabica (Coffee) Seed Extract 0.00700000 0.00700000 Carthamus Tinctorius (Safflower) Flower Extract 0.00700000 0.00700000 Polygonum Cuspidatum Root Extract 0.00700000 0.00700000 Camellia Sinensis Leaf Extract 0.00600000 0.00600000 Castanea Crenata (Chestnut) Shell Extract 0.00600000 0.00600000 Zanthoxylum Piperitum Fruit Extract 0.00600000 0.00600000 1,2-Hexanediol 0.00200000 0.00200000 Ethylhexylglycerin 0.00010000 0.00010000 Adenosine 0.04000000 0.04000000 Ammonium Acryloyldimethyltaurate/VP Copolymer 0.03000000 0.03000000 Sodium Phytate 0.01000000 0.01000000 Betaine 0.01000000 0.01000000 Allantoin 0.01000000 0.01000000 Succinic Acid 0.00100000 0.00100000 Panthenol 0.00100000 0.00100000 Pentylene Glycol 0.00100000 0.00100000 Water 0.00068490 0.00068490 Caprylic/Capric Triglycerides 0.00010000 0.00010000 Glycerin 0.00010000 0.00010000 Glyceryl Citrate/Lactate/Linoleate/Oleate 0.00002000 0.00002000 Cetyl Ethylhexanoate 0.00002000 0.00002000 1,2-Hexanediol 0.00002000 0.00002000 Butylene Glycol 0.00002000 0.00002000 Taraxacum Officinale (Dandelion) Leaf Extract 0.00001000 0.00001000 Prunus Persica (Peach) Flower Extract 0.00001000 0.00001000 Polyglyceryl-10 Myristate 0.00001000 0.00001000 Cholesterol 0.00000200 0.00000200 Ethyl Linoleate 0.00000100 0.00000100 Ceramide NP 0.00000100 0.00000100 Lactobacillus Ferment Lysate 0.00000100 0.00000100 Palmitic Acid 0.000000055 0.000000055 Stearic Acid 0.000000045 0.000000045 Water 0.00008750 0.00008750 Butylene Glycol 0.00001000 0.00001000 1,2-Hexanediol 0.00000200 0.00000200 Sodium Guaiazulene Sulfonate 0.00000050 0.00000050 Ceramide NP 0.00010000 0.00010000 Sum 100.00000000 100.00000000

[Comparative Example]

It was prepared in the same manner as in Example 1, but a composition of Comparative Example was prepared by mixing the same amount of plum pulp extract (extraction solvent: ethanol) instead of the double fermented extract of immature plums.

[Experimental Example 2: Measurement of Type I Collagen Synthesis]

The amount of type I collagen synthesis of each of the compositions prepared in the above Examples and Comparative Examples was measured. First, after dispensing and culturing HDFa cells at a concentration of 1×10 ⁴ cells/well in a 6-well plate, a control medium was used, each composition sample was treated with 2%, and cultured for 12 hours. Thereafter, after culturing for 12 hours by treatment with 200 mJ of UVB, the culture medium was centrifuged at 800 rpm for 5 minutes, and the synthesis amount was measured using a Procollagen Type I C-peptide ELA kit. The results are shown in Table 3 below.

division UVB Treatment concentration (w/v%) Amount of type I collagen synthesis (%) Control - - 100.00 Negative control + - 68.15 Example 1 + 2.00 91.41 Example 2 + 2.00 93.94 Example 3 + 2.00 97.04 comparative example + 2.00 732

As shown in Table 3, in the case of the composition of Example, it was confirmed that there is an effect of skin moisturizing and skin wrinkle improvement by exhibiting an excellent amount of collagen synthesis compared to the composition of Comparative Example.

[Experimental Example 3: Sensory Evaluation]

prepared in the above Examples and Comparative Examples Satisfaction, including the skin moisture absorption rate, moisture retention, skin regeneration, and skin trouble improvement effects of the composition, was confirmed through evaluation. The experiment was conducted on 90 men and women between the ages of 30 and 45 who met the selection criteria and were not included in the exemption criteria. They were divided into 15 people each and applied once a day for 4 weeks as a self-application method. The degree of preference felt by the subject was evaluated. The evaluation criteria were very good (5 points), excellent (4 points), average (3 points), poor (2 points), and very poor (1 point). Table 4 shows.

evaluation items Example 1 Example 2 Example 3 comparative example moisturizing skin 4.3 4.5 4.8 2.8 skin suppleness 4.0 4.2 4.6 3.2 no skin irritation 4.8 4.9 5.0 1.7 Skin trouble improvement 3.6 3.7 3.7 2.3 skin regeneration 3.6 3.9 4.2 3.0 product satisfaction 4.2 4.2 4.6 2.4

As shown in Table 4, in the case of the composition prepared according to the present invention, unlike the comparative example, most of the subjects were average compared to the overall satisfaction including skin moisturizing, absorption rate, skin regeneration and skin trouble improvement, non-irritation, etc. It was evaluated above.

[Experimental Example 4: Stability Evaluation]

The stability of the compositions prepared in Examples and Comparative Examples was evaluated. In the stability evaluation, it was visually determined whether component separation occurred when the prepared compositions were left at room temperature (20° C.) and above room temperature (35° C.) for 2 months, respectively. The criterion was described as 'unstable' when the separation of components occurred and 'stable' when the separation did not occur. The stability evaluation results are shown in Table 5 below.

temperature Example 1 Example 2 Example 3 comparative example 20℃ stability stability stability stability 35℃ stability stability stability Instability

As shown in Table 5, the compositions of Examples according to the present invention, unlike the compositions of Comparative Examples in which separation occurred when left at a high temperature (35 ° C.) for 2 months, separation occurred even during long-term storage at room temperature and above room temperature. It was confirmed that it is stable without

In this specification, only a few examples of various embodiments performed by the present inventors are described, but the technical spirit of the present invention is not limited or limited thereto, and can be modified and implemented in various ways by those skilled in the art, of course.

Claims (14)

0.001 to 1% by weight of fermented immature plum extract; and
A cosmetic composition comprising, as an active ingredient, blackcurrant juice in an amount of 0.1 to 0.3 parts by weight based on 100 parts by weight of the fermented immature plum extract;
The immature plum fermented extract is obtained by sequentially inoculating immature plums with Bacillus subtilis and lactic acid bacteria, followed by double fermentation at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 for 10 to 20 days. characterized by
The cosmetic composition is characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof.
delete The method of claim 1, wherein the immature plums,
A cosmetic composition, characterized in that it is the flesh of an immature plum fruit from which the skin and seeds have been removed.
delete The method of claim 1, wherein the cosmetic composition,
Soft lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, lotion, essence, pack, skin adhesive patch, skin adhesive gel, powder, ointment, paste, gel, suspension, emulsion, spray, serum, capsule, and Characterized in that formulated with one or more selected from the group consisting of combinations thereof, a cosmetic composition.
0.001 to 1% by weight of fermented immature plum extract; and
A quasi-drug composition comprising, as an active ingredient, blackcurrant juice in an amount of 0.1 to 0.3 parts by weight based on 100 parts by weight of the fermented immature plum extract,
The immature plum fermented extract is obtained by sequentially inoculating immature plums with Bacillus subtilis and lactic acid bacteria, followed by double fermentation at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 for 10 to 20 days. characterized by
The quasi-drug composition is characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof.
delete The method of claim 6, wherein the quasi-drug composition,
Characterized in that it is formulated with one or more selected from the group consisting of external agents, powders, sterilization disinfectants, toothpastes, ointments, lotions, internal preparations, wet tissues, sprays, pastes, bands, patches, and combinations thereof, Quasi-drug composition.
0.001 to 1% by weight of fermented immature plum extract; and
A food composition comprising, as an active ingredient, blackcurrant juice in an amount of 0.1 to 0.3 parts by weight based on 100 parts by weight of the fermented immature plum extract;
The immature plum fermented extract is obtained by sequentially inoculating immature plums with Bacillus subtilis and lactic acid bacteria, followed by double fermentation at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 for 10 to 20 days. characterized by
The food composition is characterized in that at least one selected from the group consisting of skin whitening, skin trouble improvement, skin moisturizing, skin wrinkle improvement, skin antioxidant, and combinations thereof.
delete A health functional food comprising the food composition of claim 9.
(a) preparing an immature plum extract;
(b) Bacillus subtilis and lactic acid bacteria are sequentially inoculated into the immature plum extract as a fermentation strain and double fermented for 10 to 20 days at a temperature range of 20 ° C to 40 ° C and a pH range of 4 to pH 6 Obtaining a fermented product;
(c) removing the fermentation strain from the fermentation product;
(d) aging the fermented product from which the fermentation strain was removed at a temperature range of 10 ° C to 20 ° C and a humidity range of 70% to 90% for 10 to 30 days to obtain an immature fermented plum extract; and
(e) aging the fermented immature plum extract in a crypt at a temperature ranging from 10° C. to 15° C. for 30 days to about 90 days;
Containing, a method for producing an immature plum fermented extract.
The method of claim 12, wherein the immature plum extract,
A method for producing an extract of fermented plums of immature plums, characterized in that the extract is extracted from the unripe fruits and pulp of plums from which the skins and seeds have been removed.
delete

Images