KR102019632B1 - Cosmetic composition for anti-aging containing extract of unripe fruits - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed extract of unripe fruits as an active ingredient. According to the present invention, provided is a cosmetic composition containing, as an active ingredient, a mixed extract of any one of the unripe fruits selected from the group consisting of unripe mandarin, unripe apple, unripe tomato, unripe strawberry, unripe banana, unripe lemon, unripe persimmon, unripe fig, unripe jujube and unripe plum. The cosmetic composition containing the mixed extract of unripe fruits as an active ingredient exhibits excellent skin wrinkle reduction, skin elasticity improvement, skin regeneration and stimulation relief effects.

Description

Cosmetic composition for anti-aging containing extracts of immature fruit

The present invention relates to an anti-aging cosmetic composition containing an immature fruit mixture extract, specifically from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum It relates to a cosmetic composition having an excellent anti-aging activity by containing any one of the selected mixed extract as an active ingredient.

All living things go through the process of aging from birth, which is an integral part of life. As the skin ages, aging progresses, and the first place where aging occurs is the face. Skin aging is classified into endogenous aging and photoaging caused by exposure to environmental factors. Environmental factors include ultraviolet rays, fine dust, smoking, etc. In particular, sunlight is known as a representative factor causing skin aging. Endogenous aging is mainly caused by free radicals produced by the body's metabolic processes, and the accumulation of free radicals causes skin damage and promotes aging.

Wrinkles on the skin are a common symptom of aging skin, and as you age, the amount of collagen produced in your body decreases, resulting in an increase in wrinkles on your face and a loss of elasticity and volume. Therefore, maintaining the amount of collagen is important to slow skin aging. Collagen is a protein that makes up the bulk of the dermis below the skin's epidermis, helping cells in the skin support each other. Elastin, an elastic fiber component that is involved in skin elasticity with collagen, is located in the skin tissue along with collagen, making skin tissue soft and elastic. Thus, as the skin ages, the production of collagen and elastin decreases, forming wrinkles and leading to decreased skin elasticity, which results in an aging face.

As society develops, the elderly population increases and the desire to keep skin healthy and young increases the interest in managing healthy skin and aging skin. In this interest, the management of skin aging is developing rapidly.

In this regard, research has recently been conducted to utilize immature fruits, which are not commercially available and are not used for food, that are discarded entirely. Korean Unexamined Patent Publication No. 10-2006-0104054 "Whitening cosmetic composition containing immature citrus extract" has the effect that the immature citrus extract inhibits melanin production and inhibits pigmentation, improving blemishes or freckles and skin whitening It is disclosed that the effect is excellent ', and the Republic of Korea Patent Publication No. 10-2011-0066042 "Cosmetic composition containing the unripe fruit extract" has the higher inhibitory activity of tyrosinase than the unripe fruit extract. It is disclosed that the Republic of Korea Patent No. 10-1194767 "Cosmetic composition for inhibiting pores contraction and sebum secretion containing a green apple extract" is a green apple extract, in particular the ethyl acetate fraction of the green apple peel has a pore shrinkage effect and sebum secretion suppression It is disclosed that the effect is excellent, and the Republic of Korea Patent Publication No. 10-2014-0141188 "with foot extract The oil-derived external preparation composition "discloses that the foot extract has an effect of reducing skin pores and reducing sebum secretion.

The present inventors prepared a mixed extract of various immature fruits while searching for a natural product having anti-aging activity, and studied the activity, wherein the mixed extract of green mandarin, green apple and put tomato has excellent anti-aging effect compared to the respective extracts. When it is confirmed that it has a specific extract with other immature fruits, the activity is further increased to show an excellent skin improvement effect, so that it can be usefully used as an anti-aging cosmetics to complete the present invention. It was.

The present invention contains a mixed extract of any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum as excellent ingredients It is an object to provide a cosmetic composition having aging activity.

In order to achieve the above object, according to the present invention, a mixed extract of any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum There is provided a cosmetic composition containing as an active ingredient. At this time, the mixed extract as an active ingredient is contained 0.01 to 90% by weight relative to the total weight of the cosmetic composition.

Preferably, the mixed extract is any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum. The mixture is extracted by mixing at a weight ratio of ˜2: 1 to 2: 1-2, and most preferably, is mixed and extracted at a weight ratio of 2: 2: 2: 1.

The mixed extract is hot water extract or from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether Solvent extract with at least one solvent selected.

The cosmetic composition is for skin wrinkles and elasticity improvement, skin cell regeneration or skin irritation relief.

The mixed extract of any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum according to the present invention Improvement, skin cell regeneration and skin irritation alleviating effect is excellent can be used as an anti-aging cosmetics.

Hereinafter, the present invention will be described in more detail.

The present invention is a cosmetic comprising a mixed extract of any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum It relates to a composition.

The green citrus (Citrus unshiu) used in the present invention refers to citrus fruits that are less ripe and green. It is known that green tangerine is more than twice as high in total polyphenols and pong flavonoids as compared to ripe tangerines and contains 1.5 to 2% of citric acid, which breaks down lactic acid, a substance that causes fatigue.

Unlike ripe apples, Pyrus Malus is known to contain 10 times higher polyphenol content and is rich in antioxidants, which prevents skin aging by preventing cell oxidation and cell aging. Green apple's astringent taste, tannin, rutin is effective in removing free radicals and shrinking pores, which helps to prevent skin aging.

Tomato (Solanum Lycopersicum) is a major source of lycopene for its antioxidant and anti-inflammatory effects and is also known as a longevity food. Puttomato is hard, rich in taste and contains a lot of tomate, tomatine and tomatedenole, which promotes muscle growth and prevents muscle wasting from aging.

Strawberry (Fragaria Ananassa) is a perennial herb of the dicotyledon plant Lipanaceae Rosaceae. Strawberry has a good balance between sour and sweet and contains abundant vitamin C. It contains not only anthocyanins but also various antioxidants such as flavonoids and polyphenols. Such antioxidants are known to inhibit cancer-causing cells produced by substances such as free radicals, and have functions such as antimutagenicity and cholesterol lowering action. On the other hand, the type or content of these functional phytochemicals contained in the plant also depends on the degree of maturity of the fruit. In the present invention, green strawberry means the fruit before ripening in red.

Banana (Musa Paradisica) is a generic name for plants belonging to the genus Banana, the monocotyledonous plant. Footbanana refers to an unripe green banana fruit, which is known to be beneficial to our body because it contains a lot of resistant starch, and starch, which is the most important component of water-soluble dietary fiber, is gradually It is known to be absorbed into the body and provide energy for a long time. Bananas contain vitamins C and B6 to help prevent aging by protecting against free radicals that cause premature aging of the skin.

Lemon (Citrus Limon) is a representative fruit rich in vitamin C. Vitamin C is a representative antioxidant vitamin that prevents the aging of the human body, which plays a role in delaying aging by preventing the oxidation of cells. Rich in minerals to prevent aging of the skin.

Diospyros Kaki's astringent taste of tannin has an excellent effect on astringent action, strengthening the skin and protecting the skin from free radicals with strong antioxidant power. Persimmon is rich in vitamin A, which boosts immunity and makes skin elastic. Green means a mature green persimmon.

Ficus carica L. is a subtropical semi-arictic deciduous broad-leaved plant belonging to the Moraceae family, with more than 600 varieties distributed throughout the world. Figs are rich in carbohydrates, proteins, vitamins, and minerals, and they contain antioxidants because they contain polyphenols. Figs help prevent skin aging and increase elasticity, and vitamins C and E in figs also help whitening. The extract of green fig has excellent protease activity and is being used as a meat softener for food processing.

Jujube is the fruit of the sea buckthorn and Zizyphus jujuba Mill. It contains a variety of vitamins, minerals, and beta-carotene, known for its excellent antioxidants, which boost metabolism and help prevent and prevent aging. It is initially green and ripens reddish brown or reddish brown in September-October. Jujube is known for its detoxification and anti-inflammatory properties, which help remove inflammation and alleviate pain in the body. Green jujube used in the present invention means an unripe green fruit.

Prunus Salicina is rich in vitamin C and soluble dietary fiber, which is good for skin care. Vitamin E in plum has excellent effects on anti-aging effect by blocking harmful free radicals. The green plum used in the present invention means a raw green plum fruit.

According to one embodiment of the present invention, the immature fruit mixed extract is prepared by the following method.

Washed and dried immature fruits, green apples, green tomatoes and green berries, green bananas, green lemons, green persimmons, green figs, green jujubes and green plums, 1-2 to 1-2: 1 each. Extract by mixing in a weight ratio of ˜2: 1 ~ 2. It showed excellent skin improvement effect in the above mixing ratio. At least one solvent selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether Solvents may be used. After extraction, sterile, filtered and concentrated to produce a mixed extract.

The mixed extract of the immature fruits prepared as described above had an excellent effect of increasing the expression of wrinkle-related factors (Test Examples 1 and 3), the effect of inhibiting the expression of wrinkle-forming factors (Test Examples 2 and 4), and the effect of increasing the expression of elastic factors (Test Example 5). , Inhibitory effect on elasticity-related factors (Test Example 6), skin cell regeneration effect (Test Example 7) and skin irritation mitigating effect (Test Example 8).

The mixed extract is any one immature fruit selected from the group consisting of green mandarin, green apple, green tomato and green strawberry, green banana, green lemon, green persimmon, green fig, green jujube and green plum each of 2: 2: 2: 1 When the mixture is extracted by weight ratio it showed a more excellent effect. Among them, green tea, green apple, green tomato and green strawberry were the most excellent effects when extracted by mixing at a weight ratio of 2: 2: 2: 1, respectively.

The cosmetic composition containing the immature fruit mixture extract of the present invention may be prepared in any formulation that is commonly prepared, such as lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body Gels, shampoos, rinses, hair conditioners, hair gels, foundations, lipsticks, mascaras, makeup bases, mask sheets, sun products, soaps, and the like.

EXAMPLE

Hereinafter, the present invention will be described in detail with reference to the following examples and test examples, but the present invention is not limited to these examples.

Example 1 Preparation of Green Tangerine, Green Apple, Green Tomato and Green Strawberry Mix Extract

3 g of dried green mandarin orange, 3 g of green apple, 3 g of green strawberry, 3 g of puttomato (dry weight ratio = 1: 1: 1: 1) were added to a sample of 70% hydrous ethanol, and the extractor was equipped with a cooling condenser. After boiling for 8 hours to extract the extract was filtered using a filter paper (Qualitive Filter Papers NO.2) and obtained in the filtrate state.

Examples 2-11: Preparation of green mandarin, green apple, green tomato and green strawberry mixed extract

To prepare a mixed extract by extracting and filtering in the same manner as in Example 1 by varying the mixing weight ratio of green mandarin, green apple, green tomato and green strawberry as shown in Table 1.

(Weight ratio) Example 2 3 4 5 6 7 8 9 10 11 Green mandarin One One One 2 2 2 2 2 2 2 Green apple One One 2 One One One 2 2 One 2 Foot tomato One 2 One One One 2 One One 2 2 Berry 2 One One One 2 One One 2 2 One

Comparative Example 1: Preparation of Green Tangerine Extract

12 g of dried tangerines were put in 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO. 2) to obtain a filtrate.

Comparative Example 2: Green Apple Extract Preparation

12 g of dried green apples were put in 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO. 2) to obtain a filtrate.

Comparative Example 3: Put tomato extract

12 g of dried tomato was placed in 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, filtered using filter paper (Qualitive Filter Papers NO. 2), and obtained in a filtrate state.

Comparative Example 4: Preparation of the strawberry extract

After drying 12g of dried strawberry in 1Kg of 70% hydrous ethanol and extracted by boiling at 100 ℃ in an extractor equipped with a cooling condenser for 8 hours, filtered using a filter paper (Qualitive Filter Papers NO.2) to obtain a filtrate.

Test Example 1 Confirmation of Wrinkle Related Factor Expression Increase Effect

Fibroblasts (HDFa) were adjusted to 5 × 10 ⁶ cells / well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated into 6 well plates and incubated for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the expression rate of collagen was increased through reverse transcription polymerase chain reaction (RT-PCR). Collagen expression increase rate was calculated by the following formula, the results are shown in Table 2 below. Retinol palmitate was used as a control.

Collagen expression rate (%) = (sample collagen expression amount / control collagen expression amount) × 100

Sample name Treatment concentration
(w / v%) collagen expression rate
(%) Green fruit, green apple, green tomato and green strawberry mixed extract (Example 1) 0.5 88.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 2) 0.5 89.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 3) 0.5 88.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 4) 0.5 88.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 5) 0.5 89.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 6) 0.5 87.9 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 7) 0.5 89.3 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 8) 0.5 89.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 9) 0.5 89.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 10) 0.5 90.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) 0.5 94.2 Green Tangerine Extract (Comparative Example 1) 0.5 85.2 Green apple extract (Comparative Example 2) 0.5 85.9 Foot Tomato Extract (Comparative Example 3) 0.5 84.6 Green Strawberry Extract (Comparative Example 4) 0.5 82.2 Retinol palmitate 0.01 83.8

When the same concentration as confirmed in Table 2, the control extract and each immature fruit extract in Examples 1 to 11 regarding the mixed extract extracted by mixing the green berries, green apples, green apples and green tomatoes here It showed collagen expression rate superior to Comparative Examples 1-4. Through this, it was confirmed that the synergistic effect by the mixture. Among them, the best collagen expression rate was shown in the mixed extract of Example 11, which was extracted by mixing green fruits, green apples, green tomatoes, and green berries in a weight ratio of 2: 2: 2: 1.

Test Example 2 Inhibitory Effect of Wrinkle Formation Factor Expression

Fibroblasts (HDFa) were adjusted to 5 × 10 ⁶ cells / well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated into 6 well plates and incubated for 24 hours. After incubation, the cells were irradiated with UVB, and then the samples of the examples and the comparative examples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the expression inhibition rate of MMP-1 was confirmed by RT-PCR (Reverse transcription polymerase chain reaction). MMP-1 expression inhibition was calculated by the following formula, the results are shown in Table 3 below. Retinol palmitate was used as a control.

MMP-1 expression rate (%) = (sample MMP-1 expression amount / control MMP-1 expression amount) x 100

Sample name Treatment concentration
(w / v%) MMP-1 expression rate (%) Green fruit, green apple, green tomato and green strawberry mixed extract (Example 1) 0.5 43.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 2) 0.5 43.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 3) 0.5 44.3 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 4) 0.5 42.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 5) 0.5 43.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 6) 0.5 40.4 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 7) 0.5 40.1 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 8) 0.5 43.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 9) 0.5 44.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 10) 0.5 44.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) 0.5 32.5 Green Tangerine Extract (Comparative Example 1) 0.5 50.2 Green apple extract (Comparative Example 2) 0.5 51.3 Foot Tomato Extract (Comparative Example 3) 0.5 50.5 Green Strawberry Extract (Comparative Example 4) 0.5 52.4 Retinol palmitate 0.01 40.5

Comparative Example 1 regarding the control and each immature fruit extract in Examples 1 to 11 regarding the mixed extract extracted by mixing green mandarin, green apple, green tomato and green strawberry during the same concentration treatment as confirmed in Table 3 MMP-1 expression inhibition effect was better than ~ 4. In particular, Example 11 showed the lowest expression rate of MMP-1.

Examples 12-17: Preparation of immature fruit mixed extract including green mandarin orange, green apple, green tomato

In the preparation of the mixed extract of the above example, in place of the foot strawberry of Example 11, as in the composition of Table 4, the foot banana, green lemon, green persimmon, green fig, green jujube and green plum each the same as in Example 1 Extraction and filtration by the method to prepare a mixed extract.

(Weight ratio) Example 12 13 14 15 16 17 Green mandarin 2 2 2 2 2 2 Green apple 2 2 2 2 2 2 Foot tomato 2 2 2 2 2 2 Footbanana One - - - - - Foot lemon - One - - - - Foot - - One - - - Green fig - - - One - - Green jujube - - - - One - Green plum - - - - - One

Comparative Example 5: Footbanana Extract Preparation

12 g of dried fuvana was put in 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and then filtered using a filter paper (Qualitive Filter Papers NO.

Comparative Example 6: Preparation of Foot Lemon Extract

12 g of dried foot lemon was added to 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO.

Comparative Example 7: Preparation of green persimmon extract

12 g of dried green persimmons was added to 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO.

Comparative Example 8: Preparation of green fig extract

12 g of dried green figs were put in 1 Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO.

Comparative Example 9: Preparation of Green Jujube Extract

12g of dried green jujube was placed in 1Kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and then filtered using a filter paper (Qualitive Filter Papers NO.

Comparative Example 10: Preparation of Green Plum Extract

12 g of dried green plums were placed in 1 kg of 70% hydrous ethanol, extracted by boiling at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser, and filtered using a filter paper (Qualitive Filter Papers NO.

Experimental Example 3: Confirmation of wrinkle-related factor expression increase effect

Fibroblasts (HDFa) were adjusted to 5 × 10 ⁶ cells / well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated into 6 well plates and incubated for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the expression rate of collagen was increased through reverse transcription polymerase chain reaction (RT-PCR). Collagen expression increase rate was calculated by the following formula, the results are shown in Table 5 below. Retinol palmitate was used as a control.

Collagen expression rate (%) = (sample collagen expression amount / control collagen expression amount) × 100

Sample name Treatment concentration
(w / v%) collagen expression rate
(%) Green fruit, green apple, green tomato and green banana mixed extract (Example 12) 0.5 87.6 Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) 0.5 87.5 Green fruit, green apple, green tomato and green persimmon extract (Example 14) 0.5 86.9 Green fruit, green apple, green tomato and green fig mixed extract (Example 15) 0.5 88.1 Green fruit, green apple, green tomato and green jujube extract (Example 16) 0.5 87.9 Green fruit, green apple, green tomato and green plum mixture extract (Example 17) 0.5 88.2 Footbanana Extract (Comparative Example 5) 0.5 80.2 Foot Lemon Extract (Comparative Example 6) 0.5 81.5 Foot persimmon extract (comparative example 7) 0.5 81.7 Green Fig Extract (Comparative Example 8) 0.5 81.9 Green Jujube Extract (Comparative Example 9) 0.5 80.3 Green Plum Extract (Comparative Example 10) 0.5 83.1 Retinol palmitate 0.01 83.8

As shown in Table 5, at the same concentration treatment, any one immature fruit selected from the group consisting of green mandarin, green apple and green tomato, and foot banana, green lemon, green persimmon, green fig, green jujube and green plum The mixed extracts of Examples 12 to 17 extracted by mixing showed better collagen expression rates than the immature fruit extracts (Table 1 and Table 5) of the control and comparative examples by the synergistic effect.

Experimental Example 4: Inhibitory Effect on Wrinkle Formation Factor Expression

Fibroblasts (HDFa) were adjusted to 5 × 10 ⁶ cells / well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated into 6 well plates and incubated for 24 hours. After incubation, the cells were irradiated with UVB, and then the samples of the examples and the comparative examples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the expression inhibition rate of MMP-1 was confirmed by RT-PCR (Reverse transcription polymerase chain reaction). MMP-1 expression inhibition rate was calculated by the following formula, the results are shown in Table 6. Retinol palmitate was used as a control.

MMP-1 expression rate (%) = (sample MMP-1 expression amount / control MMP-1 expression amount) x 100

Sample name Treatment concentration
(w / v%) MMP-1 expression rate (%) Green fruit, green apple, green tomato and green banana mixed extract (Example 12) 0.5 45.8 Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) 0.5 47.5 Green fruit, green apple, green tomato and green persimmon extract (Example 14) 0.5 48.3 Green fruit, green apple, green tomato and green fig mixed extract (Example 15) 0.5 47.2 Green fruit, green apple, green tomato and green jujube extract (Example 16) 0.5 48.0 Green fruit, green apple, green tomato and green plum mixture extract (Example 17) 0.5 47.3 Footbanana Extract (Comparative Example 5) 0.5 53.8 Foot Lemon Extract (Comparative Example 6) 0.5 55.3 Foot persimmon extract (comparative example 7) 0.5 54.6 Green Fig Extract (Comparative Example 8) 0.5 57.8 Green Jujube Extract (Comparative Example 9) 0.5 59.2 Green Plum Extract (Comparative Example 10) 0.5 55.7 Retinol palmitate 0.01 40.5

As shown in Table 6, at the same concentration treatment, any one immature fruit selected from the group consisting of green mandarin, green apple and green tomato, which consists of green banana, green lemon, green persimmon, green fig, green jujube and green plum The mixed extracts of Examples 12 to 17 extracted by mixing showed better MMP-1 expression inhibitory effect than the immature fruit extracts (Table 2 and Table 6) of the control and comparative examples by the synergistic effect.

Test Example 5: Increased expression of elasticity-related factors

Fibroblasts (HDFa) were adjusted to 1 × 10 ⁶ cell / well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), inoculated into 6 well plates and incubated for 24 hours. After incubation, the samples were treated and incubated for 48 hours. RNA was extracted from the cultured cells and the rate of increase of tropoelastin was confirmed by reverse transcription polymerase chain reaction (RT-PCR). Tropoelastin expression increase rate was calculated by the following formula, the results are shown in Table 7. Retinol palmitate was used as a control.

Tropoelastin expression rate (%) = (sample Tropoelastin expression / control Tropoelastin expression) x 100

Sample name Treatment concentration
(w / v%) Tropoelastin
Expression rate (%) Green fruit, green apple, green tomato and green strawberry mixed extract (Example 1) 0.5 84.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 2) 0.5 83.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 3) 0.5 82.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 4) 0.5 87.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 5) 0.5 88.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 6) 0.5 88.9 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 7) 0.5 87.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 8) 0.5 87.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 9) 0.5 86.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 10) 0.5 87.3 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) 0.5 92.5 Green fruit, green apple, green tomato and green banana mixed extract (Example 12) 0.5 86.9 Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) 0.5 86.5 Green fruit, green apple, green tomato and green persimmon extract (Example 14) 0.5 88.7 Green fruit, green apple, green tomato and green fig mixed extract (Example 15) 0.5 87.2 Green fruit, green apple, green tomato and green jujube extract (Example 16) 0.5 87.3 Green fruit, green apple, green tomato and green plum mixture extract (Example 17) 0.5 86.9 Green Tangerine Extract (Comparative Example 1) 0.5 84.5 Green apple extract (Comparative Example 2) 0.5 85.6 Foot Tomato Extract (Comparative Example 3) 0.5 83.9 Green Strawberry Extract (Comparative Example 4) 0.5 85.8 Footbanana Extract (Comparative Example 5) 0.5 84.3 Foot Lemon Extract (Comparative Example 6) 0.5 78.9 Foot persimmon extract (comparative example 7) 0.5 78.4 Green Fig Extract (Comparative Example 8) 0.5 77.9 Green Jujube Extract (Comparative Example 9) 0.5 78.3 Green Plum Extract (Comparative Example 10) 0.5 77.6 Retinol palmitate 0.01 88.9

When the same concentration as confirmed in Table 7, Examples 1 to 17 of the immature fruit mixed extract of the present invention exhibited better tropoelastin expression rate than the immature fruit extract of the control and Comparative Examples 1 to 10 by the synergistic effect It was. In particular, the highest tropoelastin expression rate was shown in Example 11.

Experimental Example 6: Inhibitory effect on elasticity-related factors

In order to confirm the inhibitory effect of elastase activity, an elastin congo red assay was performed. Prepare 2.5% agar containing 0.1% elastin-congo red using 0.2 M Tris HCl buffer. Make holes of the same size in Agar, mix 1800 units / ml of elastase enzyme solution with the sample, and disperse the hole for 24 hours. Elastase activity rate was calculated by the following equation by measuring the halo diameters, the results are shown in Table 8 below. Retinol palmitate was used as a control.

Elastase activity rate (%) = (sample halo meter / control halo meter) x 100

Sample name Treatment concentration
(w / v%) Elastase Activity
(%) Green fruit, green apple, green tomato and green strawberry mixed extract (Example 1) 0.5 40.8 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 2) 0.5 41.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 3) 0.5 42.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 4) 0.5 40.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 5) 0.5 43.6 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 6) 0.5 40.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 7) 0.5 41.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 8) 0.5 40.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 9) 0.5 40.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 10) 0.5 43.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) 0.5 36.5 Green fruit, green apple, green tomato and green banana mixed extract (Example 12) 0.5 44.1 Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) 0.5 42.8 Green fruit, green apple, green tomato and green persimmon extract (Example 14) 0.5 43.7 Green fruit, green apple, green tomato and green fig mixed extract (Example 15) 0.5 44.7 Green fruit, green apple, green tomato and green jujube extract (Example 16) 0.5 46.2 Green fruit, green apple, green tomato and green plum mixture extract (Example 17) 0.5 46.1 Green Tangerine Extract (Comparative Example 1) 0.5 46.5 Green apple extract (Comparative Example 2) 0.5 47.2 Foot Tomato Extract (Comparative Example 3) 0.5 46.8 Green Strawberry Extract (Comparative Example 4) 0.5 46.5 Footbanana Extract (Comparative Example 5) 0.5 50.2 Foot Lemon Extract (Comparative Example 6) 0.5 48.9 Foot persimmon extract (comparative example 7) 0.5 48.7 Green Fig Extract (Comparative Example 8) 0.5 47.9 Green Jujube Extract (Comparative Example 9) 0.5 50.2 Green Plum Extract (Comparative Example 10) 0.5 50.1 Retinol palmitate 0.01 42.3

As confirmed in Table 8, when the same concentration treatment, the immature fruit mixed extract of Examples 1 to 17 of the present invention showed a lower elastase activity rate than the control and Comparative Examples 1 to 10. In particular, Example 11 showed the lowest elastase activity rate.

Test Example 7: Skin Cell Regeneration Effect

A wound healing assay was conducted to confirm the skin cell regeneration effect. Keratinocytes (HEKa) were adjusted to 1 × 10 ⁶ cells / well using Medium 154 medium containing Human Keratinocyte Growth Supplement (HKGK), and then inoculated into 6 well plates and incubated for 18 hours. A portion of the cells were scratched and then treated with the samples of the above Examples and Comparative Examples and incubated for 8 hours. Cell regeneration rate was calculated by the following equation by measuring the wound gap, the results are shown in Table 9 below. Madecassoside was used as a control.

Cell regeneration rate (%) = (sample cell regeneration rate / control cell regeneration rate) × 100

Sample name Treatment concentration
(w / v%) % Cell regeneration Green fruit, green apple, green tomato and green strawberry mixed extract (Example 1) 0.5 88.7 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 2) 0.5 87.9 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 3) 0.5 89.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 4) 0.5 89.9 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 5) 0.5 87.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 6) 0.5 90.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 7) 0.5 89.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 8) 0.5 88.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 9) 0.5 87.2 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 10) 0.5 86.5 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) 0.5 93.5 Green fruit, green apple, green tomato and green banana mixed extract (Example 12) 0.5 86.9 Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) 0.5 84.9 Green fruit, green apple, green tomato and green persimmon extract (Example 14) 0.5 87.5 Green fruit, green apple, green tomato and green fig mixed extract (Example 15) 0.5 85.2 Green fruit, green apple, green tomato and green jujube extract (Example 16) 0.5 86.2 Green fruit, green apple, green tomato and green plum mixture extract (Example 17) 0.5 86.3 Green Tangerine Extract (Comparative Example 1) 0.5 84.8 Green apple extract (Comparative Example 2) 0.5 85.6 Foot Tomato Extract (Comparative Example 3) 0.5 85.9 Green Strawberry Extract (Comparative Example 4) 0.5 84.8 Footbanana Extract (Comparative Example 5) 0.5 85.5 Foot Lemon Extract (Comparative Example 6) 0.5 82.4 Foot persimmon extract (comparative example 7) 0.5 85.6 Green Fig Extract (Comparative Example 8) 0.5 85.7 Green Jujube Extract (Comparative Example 9) 0.5 84.6 Green Plum Extract (Comparative Example 10) 0.5 84.9 Madecassoside 0.01 88.6

As confirmed in Table 9, Examples 1 to 17, the immature fruit mixture extract of the present invention, when treated at the same concentration, showed a superior cell regeneration effect than the Comparative Examples 1 to 10 of the control and each immature fruit extract by synergy. It was.

Examples 18-24 Preparation of Lotions Containing Mixed Immature Fruit Extracts

The lotion containing the mixed immature fruit mixture extract prepared in Examples (Examples 11 to 17) was prepared according to a conventional method in the composition and content of Table 10 below.

(weight%) Control Example 18 19 20 21 22 23 24 Green fruit, green apple, green tomato and green strawberry mixed extract (Example 11) - 2 - - - - - - Green fruit, green apple, green tomato and green banana mixed extract (Example 12) - - 2 - - - - - Green fruit, green apple, green tomato and green lemon mixed extract (Example 13) - - - 2 - - - - Green fruit, green apple, green tomato and green persimmon extract (Example 14) - - - - 2 - - - Green fruit, green apple, green tomato and green fig mixed extract (Example 15) - - - - - 2 - - Green fruit, green apple, green tomato and green jujube extract (Example 16) - - - - - - 2 - Green fruit, green apple, green tomato and green plum mixture extract (Example 17) - - - - - - - 2 glycerin 8 8 8 8 8 8 8 8 Butylene Glycol 4 4 4 4 4 4 4 4 Caprylic / Capric Triglycerides 10 10 10 10 10 10 10 10 Cetearylglucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Sorbitan stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 Cetearyl Alcohol One One One One One One One One Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Ethylhexylglycerin 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 incense 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

Test Example 8 Confirmation of Skin Stimulation Relaxation Effect

Ten skin men and women in their twenties and thirties who do not currently have skin allergy symptoms induced skin irritation through the Tape Stripping Method in the inner region of the lower arm, and then contrasted with the lotions containing Examples 18 to 24. The lotion containing the example was applied to evaluate the degree of sedation caused by the irritation. First, taping 30 times with regular Scotch tape on two inner areas of the lower arm caused skin erythema. After erythema induction, the cosmetics containing the mixed immature and extract according to the present invention were applied, and the lotion of the control example containing no mixture according to the present invention was applied. After 3 hours of application, the degree of skin irritation was evaluated.

<Stimulation of skin irritation (erythema)>

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (obvious but weak erythema) 4 (obvious erythema) 5 (severe erythema)

Sample name Skin irritation degree Left Ooh Control One One Example 18 0 0 Example 19 0 0 Example 20 0 0 Example 21 0 0 Example 22 0 0 Example 23 0 0 Example 24 0 0

As confirmed in Table 11, the lotion containing the present invention mixed immature fruit mixed extract was excellent skin irritation effect.

Claims (7)

A green tea, green apple, green tomato and green strawberry are mixed in a weight ratio of 2: 2: 2: 1, respectively, a cosmetic composition containing a mixed extract extracted as an active ingredient. The cosmetic composition of claim 1, wherein the mixed extract is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition. delete The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin wrinkles. According to claim 1, wherein the cosmetic composition is a cosmetic composition, characterized in that for improving the skin elasticity. The cosmetic composition according to claim 1, wherein the cosmetic composition is for skin cell regeneration. According to claim 1, wherein the cosmetic composition is a cosmetic composition, characterized in that for reducing skin irritation.