KR20100010315A - Cosmetic composition inhibiting skin aging and wrinkle formation - Google Patents

Abstract

PURPOSE: A functional cosmetic composition containing medicinal herb extract is provided to improve anti-oxidation, collagen synthesis, and skin elasticity. CONSTITUTION: A medicinal herb composition contains 0.001-10% of extract of Carthamus tinctorius Linne, Rose davurica Pallas, Glycyrrhiza gabra Linne, Lycium chinense Mill, Pinus densiflora Sieb & Zucc., Oenanthe javanica(BLUME.) Dc., and Camellia sinensis. The composition has a functional cosmetic product formulation. The cosmetic product is formulated in skin, lotion, cream, essence, and mask sheet.

Description

Herbal cosmetic composition having an anti-aging effect {Cosmetic composition inhibiting skin aging and wrinkle formation}

The present invention relates to a novel herbal composition having an anti-aging effect of skin, and in particular, a herbaceous extract composed of licorice extract, safflower extract, bio-heat extract, licorice extract, goji berry extract, pine needle extract, buttercup extract, and green tea extract It relates to a functional cosmetic composition having antioxidant efficacy and collagen synthesis promotion, skin elasticity enhancing effect.

As the skin ages, it may be accompanied by changes in skin dryness, wrinkles, and skin relaxation. This can be explained as a nutritional disorder of the skin caused by loss of the capillary network of the dermis due to aging and overexposure to ultraviolet light. Therefore, the skin can be said to properly maintain the elasticity and moisturizing of the skin through a smooth supply of blood. The following two cases can be considered for smooth blood supply. One is that there must be enough blood, and the second is that the blood must circulate smoothly. This is explained in Chinese medicine and Gi (氣) and blood (血).

Our body has five viscera and the sense organs of the skin, skeletal, muscles, and five senses, and has a circulatory system called meridians that connects the five viscera and other organs, including the skin. The role of the five intestines is to change the foods we ingest into substances that our bodies need, such as Gi and blood. It is sent through the system to the skin and other organs. Based on this, other organs, including the skin, are nourished to perform normal functions and metabolic processes and form a circulatory system that sends metabolites back to the five intestines through meridians. As a person ages, he becomes deficient in blood. Of course, Ki also tends to weaken. The weakening and lack of blood donation affects many parts of the body. Among these changes in appearance can be seen to be noticeable first, and the change caused by this appears as skin sagging due to dry skin, wrinkles, decreased elasticity, and pigmentation. Therefore, in oriental medicine, two methods are used simply to make the skin healthy. The former method is to supply blood by taking drugs internally, and the other is to supply blood directly to the skin externally. . Recently, various functional herbal cosmetics combining oriental medicine and modern science have been developed using the latter method.

The herbal medicine prescription Jinguidan to be described in the present invention was prescribed as a licorice extract, safflower extract, biothermal ear extract, licorice extract, gojija extract, pine leaf extract, buttercup extract, green tea extract, etc. It is the essence that can remain as it is without getting old. ”

According to the present invention, there is provided a functional cosmetic composition of a herbal medicine safe for the skin, which exhibits an antioxidant effect and promotes collagen synthesis and promotes skin elasticity, thereby showing an excellent effect on preventing skin aging.

The present invention uses a herbal composition to solve the above problems.

Hereinafter, the herbal medicine mixed extract contained in the composition of the present invention is an extract of Licorice extract, safflower extract, bio-ear ear extract, licorice extract, goji berry extract, pine leaf extract, buttercup extract, green tea extract, which have antioxidant and collagen synthesis ability and skin elasticity enhancing effect. It is characterized by the configuration.

The herbal ingredients contained in the composition of the present invention will be described below.

Licorice (Glycyrrhiza gabra Linne) is a medicinal plant belonging to the legumes (Leguminosae). Contains various active ingredients such as licuraside and rhamnoliquiritin. In oriental medicine, it is used for the treatment of embryonic pain, fever, cough, sore throat, gastric ulcer, sensation, poisoning, dizziness, diseases of liver and uterine system, bronchitis, eczema, chronic gastritis and diarrhea. It is known that there is a whitening effect by tyrosinase inhibition.

Gojija (Lycium chinense Mill) is the fruit of goji berry tree and contains vitamin B1, B2, C, carotene, betaine, zeaxanthin, pisalien, etc. In oriental medicine, nourishing tonic action and hepatocellular growth promotion It has been shown to have an effect on the action (肝 細胞 新生 促進 作用), and skin efficacy has been reported to be excellent in removing skin exfoliation, protecting skin from ultraviolet rays and harmful oxygen, strengthening immunity, promoting and activating metabolism.

Camellia sinensis, which belongs to theaceae, is a tea made from unfermented tea leaves, which is consumed by many people, and contains various active ingredients such as quercetin, quercitrin, rutin, salicylic acid, and tannin. Due to the large amount of flavonoids, it is known to have noxious oxygen removal effect, cell activation, skin immunity strengthening effect, antibacterial action, anti-inflammatory action, antioxidant action, and skin aging inhibitory effect.

Oenanthe javanica (BLUME.) Dc. Belongs to the Apiaceae family and has a distinctive flavor and alkaline characteristics. In oriental medicine, leaves and stems are used as a medicine called carpal (水芹). The chest is stuffy with high fever and is effective for severe thirst symptoms, and it has a diuretic effect to remove swelling. Buttercup contains vitamins A, B1, B2, C, and a large amount of minerals such as ca, P, Fe, and has been reported to have harmful oxygen removal, anti-allergic and anti-inflammatory effects.

Rose davurica Pallas, a deciduous shrub of the family Rosaceae, is a medicinal crop that has been used for indigestion and menstrual disorders since ancient times, and contains large amounts of vitamins A and C. In addition, it is known to be excellent in cell and skin regeneration, anti-aging effect, skin sedation effect, whitening effect.

Pine leaf (Pinus densiflora Sieb & Zucc.) Is a leaf of the genus plant such as pine, and it is effective for preventing and treating epidemic colds and encephalitis. Among the components of pine needles, vitamins A and C have been reported to have an excellent effect on cell regeneration. In addition, pine needles are known to be effective in cell activation due to astringent action and various minerals.

Safflower (Carthamus tinctorius Linne) is a biennial herb of the Asteraceae family, also known as hongram (람), safflower (이 花), birch, and safflower. In oriental medicine, safflower seeds contain a lot of linoleic acid, which is used for the prevention and treatment of arteriosclerosis caused by excessive cholesterol, and the flower part of safflower is known to be good for menstruation and to remove blood and promote blood circulation. Linolenic acid and oleic acid, essential fatty acids contained in safflower, are known to inhibit skin aging and to activate cells by promoting skin softening and blood circulation.

In the present invention, the amount of the herbal mixture mixed with the extract having the above-mentioned efficacy may be blended in an amount of 0.0001 to 20% by weight, preferably 0.001 to 10% by weight, for the stability of the formulation.

It may have a formulation of a conventional functional cosmetic of the present invention, for example, it may be formulated into a skin, lotion, cream, essence, mask sheet and the like.

[Example 1. Juanjin raw material manufacturing method]

1-1. Noble ingredients

2L of distilled water was added to 100g of the licorice, safflower, green radish, licorice, wolfberry, pine needles, buttercup, and green tea dried samples, followed by hot water extraction for 3 hours, followed by filtration to remove the residue. The hot water extract was separated and purified, and the extract was appropriately prepared by diluting it in 1,3-Butylene Glycol to obtain a herbal extract (rare).

Experimental Example 1. Scavenging ability experiment on DPPH radicals

In order to evaluate the antioxidant efficacy of the genera, the scavenging ability of DPPH radical and the amount of GSH present in the cell were examined. The Blois method (using the reducibility of DPPH radicals) was used to measure the radical scavenging effect of the sample using DPPH (1,1-diphenyl-2-picrylhydrazyl). 0.1 mM DPPH dissolved in MeOH was added to the same amount of the raw material and reacted for 10 minutes. The DPPH radical scavenging ability of the sample was measured by measuring the absorbance at 565 nm using an absorber.

1-1. Experiment method

In 1 ml of 0.1mM DPPH solution dissolved in methanol, the samples were diluted at each concentration and 1 ml each were mixed well. After reacting for 10 minutes at room temperature, the absorbance was measured at 565 nm.

1-2. Experiment result

As a result of specifying the antioxidant activity of azaleas consisting of licorice, safflower, bio-heat, licorice, wolfberry, pine needles, buttercup, and green tea, the radical scavenging ability increased depending on the concentration and about 50% at the concentration of 0.005% (w / w) DPPH radical scavenging ability of was confirmed.

Table 1. DPPH Radical Scavenging Activity of Quercetin

DPPH radical scavenging activity of Quercetin density % Scavenging power 5 uM 25.5 ± 5.3 25 uM 87.2 ± 2.2

Table 2. DPPH radical scavenging ability measurement results of the precious metal

density % Scavenging power 0.0001% 18.8 ± 0.7 0.0005% 23.0 ± 3.2 0.001% 32.9 ± 1.2 0.005% 50.6 ± 0.4

Experimental Example 2 Evaluation of UVA Damage Defense Capacity

UVA, which penetrates into the dermis and causes major skin aging, was directly irradiated to fibroblasts to confirm that collagen synthesis ability by UVA was lowered and to investigate whether collagen synthesis was promoted. The amount of UVA (2J / cm 2) that can reduce the synthesis of collagen was treated in human-derived fibroblast CCD-986sk, and the nystagmus was treated by concentration. After 48 hours of incubation, the expression of collagen in the cell culture medium was examined by ELISA.

2-1. Experiment method

After inoculating human-derived fibroblasts (CCD-986sk) at the bottom of the culture dish, IMDM containing phenyrine (100 U / mL), streptomycin (100 μg / mL) and 10% FBS (fetal bovine serum) Iscove's Modified Dulbecco's Medium) was added and cultured in an incubator containing 37 ° C. and 5% carbon dioxide. Cells were seeded in a 35 mm culture dish to a concentration of 2 × 10 ⁵ / well and further incubated for 24 hours. After replacement with PBS buffer solution, UVA 2J / m ² was irradiated and treated with a nystagmus 0.05, 0.1% concentration and further incubated for 48 hours. The amount of collagen was measured by ELISA method using cell culture medium, and the amount of protein was corrected.

2-2. Experiment result

As a result of evaluating collagen synthesis ability of the novelty, it was confirmed that collagen synthesis capacity was increased than UVA only treatment at 0.05% concentration.

In the case of the genuine raw material, collagen synthesis ability decreased at 0.1% concentration compared to 0.05% sample, which may be due to the decrease in cell number due to the presence of some cytotoxicity at 0.1% concentration. At 0.05% concentration, it is determined that UVA protects against skin damage by UVA.

Table 3. Results of measuring collagen synthesis in control group (% of control)

sample Collagen Synthesis Control group (sample no treatment) 100 UVA 2J / m ² 42

Table 4. Results of measuring collagen synthesis ability (% of control)

             Concentration (w / v%) sample 0.05 0.1 Noble 49 43

Experimental Example 3. Measurement of Skin Elasticity

Cutometer MPA 580 (Courage Khazaka, Germany) was used to confirm the increase in skin elasticity of the sample. Cutometer measures the elasticity of skin using a physical index that expresses the difference between the degree of pulling and restoring the skin by applying a negative pressure inside the probe that is in close contact with the skin. The index from R0 to R9 depending on the physical characteristics. It expresses skin elasticity. The raw material concentrate was applied twice a day to the test site for 2 weeks to 1%, and the change in skin elasticity was measured using a cutometer 24 hours after the last sample application.

3-1. Experiment method

1) Target: 10 women in their 20s (applied to both cheeks)

2) Measurement content: skin elasticity evaluation

3) Temperature and Humidity: Temperature-21.2 ℃, Humidity-56.5%

4) Measuring Equipment: Cutometer MPA 580

5) Measuring condition

On-time Off-time Pressure Repetition Total-time 2.0 sec 2.0 sec 450 mbar 3 12 sec

3-2. Experiment result

R2 values, which represent the total elasticity of the skin (skin remodeling capacity), were compared before and after application of the sample. The closer to R2, the better the recovery rate is when the skin is pulled using negative pressure. As shown in Table 5 below, as a result of measuring the elasticity, the elasticity before the sample treatment and after the sample treatment was increased.

Table 5. Evaluation Results of Elasticity

Right ball Left ball Before 0.95 0.95 After 0.98 0.97

Experimental Example 4. Formulation Stability Check

In order to secure stability in applying cosmetic formulations of the genuine ingredient, which confirmed various skin effects, the stability against temperature and pH was confirmed.

4-1. Stability to temperature

In order to confirm the stability according to the temperature of the raw material, prepared by diluting with 1, 3, 5% aqueous solution using the raw material and incubated for 30 minutes at a temperature range of 4 ℃, room temperature, 65 ℃ was measured. As a result, it is judged that the temperature is very stable as shown in Table 6.

Table 6. Temperature Stability Evaluation Results

 V / V 4 ℃ Room temperature 65 ℃ 30min 60min 120 min 30min 60min 120 min 30min 60min 120 min One % + + + + + + + + + 3% + + + + + + + + + 5% + + + + + + + + +

                            +: Stable, ±: somewhat cloudy

4-2. stability to pH

In order to confirm the stability according to the pH of the true raw material raw material was prepared by diluting with 1, 3, 5% aqueous solution using the raw material and measured in the range from pH 2.0 to pH 10.0 at room temperature. As a result, it is determined that the pH is very stable as shown in Table 7.

Table 7. Results of pH Stability Evaluation

2 4 6 8 10 One % + + + + + 3% + + + + + 5% + + + + +

                              +: Stable, ±: somewhat cloudy

Claims (4)

Composition with a total of 0.1% to 10 herbs containing herbal extracts, safflower extracts, green heat extract, licorice extract, goji berry extract, pine leaf extract, buttercup extract, green tea extract based on the total weight of the composition. The herbal composition characterized in that the composition is characterized by the antioxidant efficacy of the skin. Herbal composition wherein said composition exhibits promoting collagen synthesis of skin. Herbal composition wherein the composition is to promote the elasticity of the skin.