JP6910751B2 - Moisturizers, anti-inflammatory agents, antioxidants, skin turnover improvers, cell activators and whitening agents - Google Patents

Description

The present invention relates to a functional material having excellent biosafety and which is blended in cosmetics (including quasi-drugs) and cosmetological oral compositions.

Skin aging is caused by internal factors such as inactivation of cell proliferation and differentiation with aging, decreased hormone secretion, and quantitative decrease of extracellular matrix components, as well as active oxygen and chemistry induced by sunlight (ultraviolet rays). It is a phenomenon that occurs when substances or external factors such as environmental stress are intricately intertwined. Among them, ultraviolet rays and chemical substances, which are external factors, may damage cells and tissues of the skin and alter biological components, resulting in the generation of antigens in the skin. From this, these external factors cause skin inflammation and allergies caused by antigens, and also induce abnormal deposition of melanin pigment to cause spots, freckles, chloasma, etc. on the skin. Inflicts damage.

In order to prevent skin aging caused by the above factors and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics and oral compositions containing these active ingredients have been proposed. Is on the market. For example, antioxidants such as vitamin C and vitamin E; anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; cell activation such as α-hydroxycarboxylic acid, placenta extract, and γ-amino-β-hydroxybutyric acid. Ingredients; extracellular matrix components such as collagen, elastin, hyaluronic acid; moisturizers such as urea have been proposed. However, it is difficult for conventional active ingredients to sufficiently satisfy both efficacy and biosafety, and there is a demand for a functional raw material having improved such points.

In view of the problems of the prior art, the present inventors have conducted diligent studies to find a new active ingredient derived from a natural product from the viewpoint of biosafety. As a result, the extract of the immature fruit of peach, which is a plant belonging to the genus Sakura (Peach) of the Rosaceae family, exhibits excellent skin-whitening and whitening effects, and by blending the extract, it is excellent in biosafety and effectiveness. We have found that it will be possible to provide cosmetics and oral cosmetic compositions.

In the process of cultivating peaches, which are Rosaceae plants, the work of thinning out immature fruits, that is, the work called "fruit thinning" is performed in order to adjust the number and size of fruit fruits. Therefore, among peaches, it has been required to effectively utilize these thinned immature fruits.

Conventionally, cosmetics containing peach seeds (peach seeds) or flower extracts, which are plants belonging to the genus Sakura (peach) of the Rosaceae family, as active ingredients are known (Patent Documents 1 and 2). Further, it is known that immature peach fruits have an elastase activity inhibitory action (Patent Document 3). Further, oral or external compositions containing polyphenols derived from immature fruits of Rosaceae plants (apples, pears, peaches) as active ingredients are also known (Patent Documents 4 and 5). However, the immature fruit extract of peach itself has a moisturizing effect, an anti-inflammatory effect, an antioxidant effect, a skin turnover improving effect, a cell activating effect and a whitening effect, and it is effective to combine these. Nothing was known about the ability to provide sexually superior cosmetics or cosmetological oral compositions.

Japanese Patent Application Laid-Open No. 11-335235 Japanese Patent Application Laid-Open No. 07-309770 Japanese Patent Application Laid-Open No. 2011-105693 Japanese Patent Application Laid-Open No. 08-259453 Japanese Patent Application Laid-Open No. 09-175982

The present invention is a moisturizer containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.
Further, the present invention is an anti-inflammatory agent containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.
Further, the present invention is an antioxidant containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.
Further, the present invention is a skin turnover improving agent containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.
Further, the present invention is a cell activator containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.
Further, the present invention is a whitening agent containing an extract of immature fruits of peaches of the genus Plum of the Rosaceae family as an active ingredient.

According to the present invention, the extract of immature fruit of peach, which is an active ingredient, is excellent due to the synergistic action of moisturizing action, anti-inflammatory action, antioxidant action, skin turnover improving action, cell activating action and whitening action. It is possible to provide a combination drug of a cosmetic or an oral composition for beauty. In addition, since the extract is derived from a natural product, it is possible to provide cosmetics and cosmetological oral compositions having excellent biosafety.

Hereinafter, the present invention will be described in detail. In this specification, the term cosmetics is used in a broad sense to include not only so-called cosmetics but also quasi-drugs.
The varieties of Plum (Prunus persica) of the Rosaceae family used in the present invention are not particularly limited. , Asama white peach, and any varieties of peach may be used.

The immature peach fruit referred to in the present invention refers to a fruit having a diameter of 0.5 to 5 cm, preferably 2 to 3 cm.

To prepare the extract, immature peach fruits are washed with water in advance to remove foreign substances, if necessary, dried as they are or dried, and then chopped or crushed as necessary, and extracted according to a conventional method such as a dipping method. This can be done by contacting with a solvent.

Extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like. Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in admixture of two or more. Used.

Among these extraction solvents, hydrophilic solvents such as water, lower alcohols and polyhydric alcohols are suitable in the present invention. Preferred examples of using this hydrophilic solvent include, for example, water or lower alcohols (particularly ethanol) used alone, a mixed solvent of water and lower alcohols (particularly ethanol), or water and polyhydric alcohols (particularly ethanol). In particular, the use of a mixed solvent with 1,3-butylene glycol or propylene glycol) can be mentioned, but the use of water alone is most preferable. When a mixed solvent is used, for example, in the case of a mixed solvent of water and ethanol, the volume ratio (hereinafter the same applies) is 1: 1 to 25: 1, and in the case of a mixed solvent of water and glycerin, 1: 1. In the case of a mixed solvent of ~ 20: 1, water and 1,3-butylene glycol, the range is preferably 1: 1 to 20: 1.

When preparing the extract, the pH of the extract is not particularly limited, but is generally preferably in the range of 4 to 8. In this sense, if necessary, an alkali adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide may be added to the extraction solvent to adjust the pH to a desired level.

Extraction conditions such as extraction temperature and extraction time differ depending on the type and pH of the solvent used. For example, when water is used as the extraction solvent, the extraction temperature is preferably in the range of 0 to 85 ° C., and the extraction time. Is preferably in the range of 2 to 3 hours.

The extract obtained under the above conditions generally has a pH adjusted to 4 to 8, and even if it is used as it is as a compounding agent for cosmetics or cosmetological oral compositions, it is used as a desired concentration by concentration under reduced pressure or the like. You may.

Since the extract prepared under the above conditions does not substantially contain polyphenols, polyphenols derived from immature fruits of Rosaceae plants (apples, pears, peaches, etc.) disclosed in Patent Documents 4 and 5 above. Is distinguished from.

Cosmetics (including non-medicinal products) containing the immature fruit extract of the peach of the present invention include, for example, basic cosmetics such as milky lotion, cream, lotion, essence, and pack, lipstick, foundation, liquid foundation, and makeup. Makeup cosmetics such as uppress powder, blusher, white powder, cleansing cosmetics such as face wash, body shampoo, soap, hair cosmetics such as shampoo, hair conditioner, hair cream, and bathing agents. However, of course, it is not limited to these. In addition, as oral cosmetic compositions, beverages such as beauty beverages, nutritional drinks, sports drinks, near water, vitamin beverages, mineral beverages, alcoholic beverages; various soups (including powdered soups), dairy products, jellies, candy , Tablets, gums and other foods; can be blended in tablets, liquids, granules or jelly-like health foods / beverages, etc. can do.

The blending amount of the immature peach fruit extract in the cosmetics is generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2 in the case of basic cosmetics, as the solid content of the extract. In the range of% by weight, in the case of make-up cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight, and in the case of cleansing cosmetics, generally in the range of 0. It is in the range of 002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight (solid content weight%, the same applies hereinafter), preferably 0.0001 to 3% by weight. It is 0.0% by weight. The blending amount of the immature peach fruit extract in the cosmetological oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

In addition to the essential ingredient peach immature fruit extract, cosmetics or cosmetological oral compositions include ingredients commonly used in cosmetics, such as oily ingredients, surfactants (synthetic and natural products), etc. Moisturizers, thickeners, preservatives / bactericides, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances and the like can be appropriately blended as needed. In addition, as long as the effectiveness and characteristics of the immature fruit extract of peach are not impaired, other physiologically active ingredients may be combined and blended at all.

Here, examples of the oily component include olive oil, jojoba oil, paraffin oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadowfoam oil, sheer butter, tea tree oil, and avocado oil. Plant-derived fats and oils such as macadamia nut oil and plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, vaseline, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Examples thereof include synthetic esters such as isopropyl, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, and poly. Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, tris. Cationic surfactants such as alkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamide propyl-N', N'-dimethyl-N'- Amphoteric surfactants such as β-hydroxypropylammoniosulfobetaine can be used.

Examples of emulsifiers or emulsifying aids include stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented grains (wheat, beans, miscellaneous grains, etc.). It can also be blended.

Examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, and saccharides such as trehalose and mucopolysaccharides (for example, hyalurone). Acids and their derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extract, silane roots Examples include extracts, various amino acids and their derivatives.

Examples of the thickener include ingredients derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white and) extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, Gum such as tragant gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, alginic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; poly Examples thereof include glutamate and derivatives thereof.

Examples of preservatives and bactericides include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophenol, hexachlorophene, chlorhexidine hydrochloride, and benza chloride. There are luconium, salicylic acid, ethanol, undesyleneic acid, phenols, jamar (imidazodeini ruurea), 1,2-pentanediol, various essential oils, and dried bark.

Examples of powder components include cericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. There are system powders, grain powders (rice, wheat, corn, millet, etc.), beans (soybeans, red beans, etc.) powders, etc.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tershally butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

Whitening agents include t-cycloamino acid derivatives, kodiic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4 -Potasium salicylate, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhododenol, 4- (4-hydroxyphenyl) -4-butanol), hydroxybenzoic acid And its derivatives, Vitamin E and its derivatives, α-hydroxyic acid, AMP (adenosine monophosphate, adenosine monophosphate), and these may be blended alone or in combination of two or more.

Examples of the ascorbic acid derivative include ascorbic acid esters such as ascorbic acid monobutyrate, ascorbic acid monocaplate, ascorbic acid monopalmitate, and ascorbic acid dibutyrate, ascorbic acid ethers, and ascorbic acid sugar derivatives such as ascorbic acid glucoside. However, examples of the ascorbic acid derivative include L-ascorbic acid-2-phosphate ester sodium, L-ascorbic acid-2-phosphate ester magnesium, L-ascorbic acid-2-sulfate sodium ester, and L-ascorbic acid-2. -Ascorbic acid ester salts such as magnesium sulfate, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, and 6-position acylated products of these ascorbic acid sugar derivatives (the acyl group is Hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitic acid ester, L-ascorbic acid tetralauric acid ester, 3-O-ethylascorbic acid, L- Ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or an acylated derivative thereof, ascorbic acid glucerin derivative such as bisglyceryl ascorbic acid, L-ascorbic acid phosphate aminopropyl, L-ascorbic acid As a hyaluronic acid derivative or the like, as a hydroquinone derivative, albutin (hydroquinone-β-D-glucopyranoside), α-albutin (hydroquinone-α-D-glucopyranoside) or the like, and as a tranexamic acid derivative, a tranexamic acid ester (for example, tranexam) Examples thereof include acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, methylamide tranexamic acid), and examples of the resorcinol derivative include 4-n-butylresorcinol. As the 2,5-dihydroxybenzoic acid derivative, for example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid and the like can be used. Examples of the nicotinic acid derivative include nicotinic acid amide and benzyl nicotinate, and examples of α-hydroxy acid include lactic acid, ascorbic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like.

Physiologically active ingredients include, for example, placenta extract, sohakuhi extract, yukinoshita extract, perilla extract, rice bran extract or its hydrolyzate, white potato extract or its hydrolyzate, white potato extract. Fermented product, Shakyaku extract or its hydrolyzate, Lactobacillus fermented rice, Murasakikibu extract, Hass seed extract or its hydrolyzate, Hass seed fermented product, Ginseng extract, Hatomugi hydrolyzate, Hatomugi seed fermented product, Royal jelly Fermented product, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. are listed, and coral grass extract is used as an anti-aging component. Extract of rice leaf extract or its hydrolyzate, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, seaweed extract such as catamen giraffe Extracts of marine flowering plants such as amamo, fermented soymilk, jellyfish water, rice extract or its hydrolyzate, fermented rice extract, sprouted rice extract or its hydrolyzate, sprouted rice fermented product, black bean extract Fermented material or its hydrolyzate, damask rose flower extract, bamboo shoot skin extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid, etc.), animal or fish-derived collagen and its derivatives, elastin And its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract , Carrot extract, Aloe extract, Mitsuishikonbu extract, Hechima extract, Anaaosa extract, Zizyphus joazeiro extract and the like.

Next, the present invention will be described in more detail with reference to Production Examples, Examples (Prescription Examples) and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Preparation of peach immature fruit extract 600 g of immature fruit of peach (Plumus persica Bach) is mixed with 600 g of purified water, and the extract solution is extracted at 80 ° C. for 2 hours in a standing state, and the extract solution is 450.1 g. Got Then, the obtained extract solution was filtered, and 1% of activated carbon (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the filtered solution to perform activated carbon treatment for 1 hour, and the light brown peach was immature. 431.0 g of a fruit extract solution was obtained (pH 4.1, solid content concentration 3.50%).

Comparative manufacturing example 1. Preparation of extract of mature peach fruit 150 g of purified water was mixed with 15 g of mature fruit (skin, dried product with seeds) of peach (Plumus persica Bach), and the mixture was left to stand and extracted for 2 hours at 80 ° C. rice field. Then, 110.5 g of the extract solution of the mature fruit of peach was obtained. Then, the obtained extract solution was filtered, and 1% of activated carbon (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the filtered solution to perform activated carbon treatment for 1 hour to extract the mature fruit of peach. A solution was obtained (pH 5.0, solid content concentration 7.30%).

Example 1. Cream [Ingredient A] Part Liquid paraffin 5.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) Sorbitan Monostearate 6.0
Phenoxyethanol 0.1
[Component B] Extract solution of Part Production Example 1 2.5
Glycerin 5.0
Carboxymethyl monostearate 0.1
Amount of purified water totaling 100 parts [C component]
Appropriate amount of fragrance The above components A and B were heated to 80 ° C. or higher, and then stirred and mixed. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2. Emulsion [Component A] Part Liquid paraffin 6.0
Olive oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
[Component B] Part The extract solution of Production Example 1 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Collagen 0.1
Amount of purified water totaling 100 parts
[C component]
Appropriate amount of fragrance The above components A and B were heated to 80 ° C. or higher, and then stirred and mixed. After cooling this to 50 ° C., the C component was added and further stirred and mixed to obtain a milky lotion.

Example 3. Lotion [Component A] Part Extraction Solution of Production Example 1 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Phenoxyethanol 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts The above components were mixed to obtain a lotion.

Example 4. Toner [A component] Part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
[Component B] Extract solution of Part Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts
[Component C] Appropriate amounts of perfume A component and B component were heated to 80 ° C. or higher, and then B component was added to A component and stirred, and homogenized with hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 ° C., the C component was added, stirred and mixed, and further cooled to 30 ° C. or lower to obtain a lotion.

Example 5. Emulsion [Component A] Part Liquid paraffin 6.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Appropriate amount of fragrance The above components A and B were heated to 80 ° C. or higher, and then stirred and mixed. After cooling this to 50 ° C., the C component was added and further stirred and mixed to obtain a milky lotion.

Production example 6. Emulsion In the B component of Example 5, 2.0 parts of L-ascorbic acid-2-phosphate ester magnesium is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide. Obtained a milky lotion in the same manner as in Examples.

Example 7. Emulsion Emulsion in the same manner as in Example 5 except that 2.0 parts of tranexamic acid is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Example 5. Got

Example 8. Emulsion Emulsion was prepared in the same manner as in Example 5 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Example 5. Obtained.

Example 9. Emulsion [Component A] Part Liquid paraffin 6.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Appropriate amount of fragrance The above components A and B were heated to 80 ° C. or higher, and then stirred and mixed. After cooling this to 50 ° C., the C component was added and further stirred and mixed to obtain a milky lotion.

Example 10. Liquid foundation [A component] Stearic acid 2.4
Propylene glycol monostearate 2.0
Setostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract solution of Production Example 1 2.0
Sodium Carboxymethyl Cellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Amount of purified water totaling 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Appropriate amount of color pigment The above components A and B were heated and then mixed and stirred. This was reheated, the above C component was added and poured into a mold, and the mixture was stirred until it reached room temperature to obtain a liquid foundation.

Example 11. Cream Foundation [Ingredient A] Part Stearic Acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalene 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[Component B] Part 2.5 Extract solution of Production Example 1
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Amount of purified water totaling 100 parts [C component] part Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Appropriate amount of coloring pigment [D component] Part fragrance 0.3
The C component was mixed and pulverized with a pulverizer. The B component was mixed, the pulverized C component was added thereto, and the mixture was uniformly dispersed with a colloid mill. After heating the A component and the uniformly dispersed B and C components to 80 ° C., the A component was added to the B and C components with stirring, and homogenization was further carried out with hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 ° C., component D was added and mixed by stirring, and the mixture was further cooled to 30 ° C. or lower with stirring to obtain a cream foundation.

Example 12. Body shampoo [A component] Part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[Component B] Part Extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts A component and B component are each heated to 80 ° C and uniformly dissolved, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a body shampoo. rice field.

Prescription example 13. Hair shampoo [A component] Part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) Alkyl Ether Sodium Sulfate 20.0
Betaine Lauryl Dimethylamino Acetate 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Amount of purified water that makes up 100 parts A component and B component are each heated to 80 ° C and uniformly dissolved, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a hair shampoo. rice field.

Example 14. Hair conditioner [Component A] Part Polyoxyethylene (10) Hardened castor oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts A component and B component were each heated to 80 ° C. to uniformly dissolve them, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. ..

Prescription example 15. Extract of Beauty Beverage Production Example 1 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (vitamin C) 0.01
Amount of purified water totaling 100 parts

Prescription example 16. Extract of Tablet Production Example 1 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After mixing each component by weight, pressure molding was performed to obtain a tablet.

Test example 1. Gene expression evaluation test of normal human epidermal cells In this test, the following tests were conducted to evaluate the effect of the extract of immature peach fruit according to the present invention on gene expression of normal human epidermal cells. ..
[Test method]
^{Normal human epidermal cells are prepared at 6 × 10 5} cells / mL with HuMedia KG2 [manufactured by Kurabo Industries] containing a growth additive, 1 mL is seeded in a φ6 cm petri dish, and cultured at 37 ° C. under _{5% CO 2 and saturated steam.} did. After culturing for 24 hours, a culture solution containing the extract (sample solution) of Production Example 1 (the extract was added so that the final concentration of the solution was 2.0% with respect to the total amount of the culture solution). Was added and cultured. In addition, as a comparative control, a control group was set in which a culture solution containing only 2.0% PBS (−) solution was added instead of the sample solution. After culturing for 24 hours, the cells of each test group were collected with 1 mL of Trizol reagent (manufactured by Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, stirred and mixed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes with a centrifuge (TOMY Co., Ltd./MX-160). After that, only the aqueous layer was separated by 400 μL. 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer, stirred and mixed, and centrifuged at 15,000 rpm for 15 minutes under the conditions of 4 ° C. to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, stirred and washed, and the precipitate was collected by centrifugation at 15,000 rpm for 15 minutes under the condition of 4 ° C. The recovered total RNA was reverse-transcribed using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser [Perfect Real Time] (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using the synthesized cDNA as a sample, using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.]. The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is commonly expressed in a certain amount in many tissues and cells, is always expressed, and is essential for cell maintenance and proliferation. It is one of the above, and since the expression level is always constant, it is used as an internal standard in PCR experiments. The test results were compared with the expression level of each gene in each test group when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of the gene in the test group was determined when the expression level of each gene in the control group was 100. The test results are shown in Tables 1-6.

[Table 1]

[Table 2]

As shown in Tables 1 and 2, the extract of Production Example 1 according to the present invention is a gene encoding hyaluronoglucosaminidase1 [HYAL1], Hyaluronoglucosaminidase2 [HYAL2], which is an enzyme that decomposes hyaluronic acid in epidermal cells. The moisturizing function of the skin can be improved by suppressing the expression of.

[Table 3]

[Table 4]

As shown in Tables 3 and 4, the extract of Production Example 1 according to the present invention is a matrix metalloproteinase (matrix metalloproteinase2 [MMP2], matrix metalloproteinase 9) which is an enzyme that decomposes type IV collagen constituting the basement membrane of epidermal cells. By suppressing the expression of the gene encoding [MMP9]), it is possible to protect the basement membrane of epidermal cells and improve the turnover of the superficial layer of the skin.

[Table 5]

As shown in Table 5, the extract of Production Example 1 according to the present invention suppresses the expression of the gene encoding the inflammatory cytokine interleukin-1 (Interleulin1 alpha [IL1A2]), and thus causes skin inflammation. And pigmentation can be suppressed.

[Table 6]

As shown in Table 6, the extract of Production Example 1 according to the present invention expresses a gene encoding nitric oxide synthase (NOS2], which is one of the active oxygen species. By suppressing it, it is possible to suppress oxidative damage to cells and further suppress skin pigmentation caused by active oxygen.

Test example 2. Gene expression evaluation test of normal human fibroblasts In this test, in order to evaluate the effect of the immature fruit extract of the peach according to the present invention on the gene expression of normal human fibroblasts, the following tests were conducted. went.
[Test method]
^{Normal human fibroblasts were prepared in 6 × 10 5} cells / mL with Eagle MEM (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 0.5% by volume NCS, and 1 mL was seeded in a φ6 cm petri dish to saturate with _{5% CO 2.} The cells were cultured at 37 ° C. under steam. After culturing for 24 hours, a culture solution containing the extract of Production Example 1 according to the present invention (the extract was added so that the final concentration of the solution was 2.0% with respect to the total amount of the culture solution). Was added and cultured. As a comparative control, the final concentration of PBS (-) with respect to the total amount of the culture solution was adjusted to 2.0% instead of the extract of Production Example 1 according to the present invention. The test group (control group) to which the product was added was set. After culturing for 24 hours, the cells of each test group were collected with 1 mL of Trizol reagent (manufactured by Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, stirred and mixed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes with a centrifuge (TOMY Co., Ltd./MX-160). After that, only the aqueous layer was separated by 400 μL. 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer, stirred and mixed, and centrifuged at 15,000 rpm for 15 minutes under the conditions of 4 ° C. to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, stirred and washed, and the precipitate was collected by centrifugation at 15,000 rpm for 15 minutes under the condition of 4 ° C. The recovered total RNA was reverse-transcribed using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using the synthesized cDNA as a sample, using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.]. The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is commonly expressed in a certain amount in many tissues and cells, is always expressed, and is essential for cell maintenance and proliferation. It is one of the above, and since the expression level is always constant, it is used as an internal standard in PCR experiments. The test results were compared with the expression level of each gene in each test group when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of the gene in the test group was determined when the expression level of each gene in the control group was 100. The test results are shown in Tables 7 to 15.

[Table 7]

As shown in Table 7, the extract of Production Example 1 according to the present invention enhances the expression of the gene encoding Hyaluronan synthase2 [HAS2], which is a synthase of dermal hyaluronic acid. It is possible to improve the firmness and the like.

[Table 8]

As shown in Table 8, the extract of Production Example 1 according to the present invention enhances the expression of the gene encoding the constituent protein of dermal elastin fiber (Elastin microfibril interfacer1 [EMILIN1]). Can be improved.

[Table 9]

As shown in Table 9, the extract of Production Example 1 according to the present invention is a gene encoding XVIII type collagen (collagen, type XVIII, alpha 1 [COL18A1]), which is a kind of proteoglycan constituting the basement membrane of the skin. Since the expression of collagen is enhanced, the synthesis of proteoglycan constituting the basement membrane can be promoted, and skin turnover and the like can be improved.

[Table 10]

As shown in Table 10, the extract of Production Example 1 according to the present invention suppresses the expression of a gene encoding an enzyme (prostaglandin I2 synthase [PTGIS]) involved in the synthesis of prostaglandin I2, which is an inflammatory substance. Therefore, skin inflammation and pigmentation can be suppressed.

[Table 11]

[Table 12]

As shown in Tables 11 and 12, the extract of Production Example 1 according to the present invention detoxifies harmful molecules and is involved in the oxidative stress response in the presence of glutaone, which is an antioxidant in the living body. By enhancing the expression of genes encoding glutathione peroxidase1 [GPX]) and glutathione-S-transferase mu2 [GSTM2], it prevents and improves in vivo detoxification effects, disorders caused by oxidative stress, and diseases. can do.

[Table 13]

[Table 14]

As shown in Tables 13 and 14, it is necessary to enhance the expression of genes encoding aconitase (Aconitase1 [ACO1], Aconitase2 [ACO2]), which are enzymes that play an important role in the intracellular energy metabolism pathway (TCA cycle). Therefore, it is possible to activate intracellular energy synthesis that decreases with age.

[Table 15]

As shown in Table 15, since it enhances the expression of a gene encoding an inhibitor of metalloproteinase inhibitor3 [TIMP3], which is an enzyme that decomposes extracellular matrix (for example, collagen, proteoglycan, elastin, etc.), The extracellular matrix can be protected.

Test example 3. ^{Fibroblast ATP synthesis enhancing effect Human dermal-derived fibroblast NB1RGB was seeded in 1 × 10 4} cells / hole in a 96-well microplate containing the minimum essential medium for eagle containing 0.5% NCS (manufactured by Nissui Pharmaceutical Co., Ltd.). After pre-culturing under the conditions of 37 ° C. and 5.0% CO ₂ for 1 day, the extract of Production Example 1 was used as a sample solution in a medium at a concentration of 2.5% (the final solution of the sample solution with respect to the total amount of the medium). It was added so as to have a concentration of), and the cells were cultured under the same conditions for another 3 days. For comparison, PBS (−) and mature peach extract were also added to a concentration of 2.5% and cultured in the same manner. Next, the medium was removed, 100 μL / hole of PBS (-) was added, 100 μL / hole of ATP luminescent reagent (Toyo Orbit) was added, and the mixture was stirred with a microplate shaker for 1 minute and then luminometer set at 23 ° C. The amount of light emitted was measured by allowing it to stand for 10 minutes at (TECAN). In this test system, the relative value of the luminescence amount in the other test groups when the luminescence amount in the PBS (−) 2.5% addition group was set to 100 was calculated and used as the ATP synthesis rate.

The results of Test Example 3 are shown in Table 16.
[Table 16]

As shown in Table 16, it was confirmed that the extract of Production Example 1 according to the present invention has a significantly superior effect of enhancing fibroblast ATP synthesis as compared with the extract of mature peach fruit of Comparative Example 1. Was done.

Test Example 4: Confirmation test for phenols (polyphenols, etc.) [Sample]
Extract solution of immature fruit of peach of Production Example 1 (Sample 1 of the present invention)
[Test method]
First, 9 g of ferric chloride (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in 100 mL of purified water to prepare a ferric chloride solution. Next, the above ferric chloride solution was added to 1 g of the sample solution, and the color development was observed.
[result]
Even when the ferric chloride solution was added to the sample 1 of the present invention, no change was observed in the color of the sample solution, and no color reaction was observed. Since ferric chloride reacts with phenols (polyphenols, etc.) and exhibits green to black-blue, it was confirmed that the extract of immature peach fruits according to the present invention does not contain phenols. rice field.

Claims (3)

Hyaluronoglucosaminidase 1 gene expression inhibitory action and hyalurono using an extract of immature fruits of peach (Prunus persica) of Rosaceae (Rosaceae) Plum (Prunus persica) as an active ingredient Glucosaminidase 2 A skin moisturizing function improving agent based on the gene expression inhibitory effect. Matrix metalloprotease gene expression inhibitory action and XVIII type collagen containing an extract of immature fruits of Plum (Prunus persica) of Rosaceae (Rosaceae) and peach (Prunus persica) as an active ingredient. A skin turnover improving agent based on gene expression enhancing action. Interleukin-1 gene expression inhibitory action and monoxide containing an extract of immature fruits of Plum (Prunus persica) of Rosaceae (Rosaceae) and Plum (Prunus persica) as an active ingredient whitening agent based on a nitrogen synthase gene expression suppression operation.