JP2000136124A - Skin lotion - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin lotion capable of keeping the skin elastic, fresh and young by controlling a skin differentiation and a skin proliferation by inhibiting an elastase activity. SOLUTION: This skin lotion comprises either or both of (1) a component comprising an oligopeptide having an amino acid sequence consisting of Val-Gly- Val-Ala-Pro-Gly, a polypeptide, a protein or an extract containing the oligopeptide and (2) a component comprising one or more kinds of extracts selected from the extract of Chlorella (Chlorella), Ascophyllum (scientific name: Ascophyllum Nodosum), Silybum marianum Gaertn (scientific name: Silybum marianum (L) Gaertn) or Centella asiatica (scientific name: Centella asiatica (L) Urb).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin, and more particularly to an external preparation for skin which produces elastic and fresh skin.

[0002]

2. Description of the Related Art In recent years, studies on skin aging have progressed rapidly, and the process of skin aging at a tissue level, a cellular level or a molecular level has been revealed. That is, it has been found that skin aging is a phenomenon that cannot be avoided at the genetic level, but is also promoted by the influence of sunlight such as ultraviolet rays and environmental factors such as drying and oxidation.

[0003] Specifically, the tissue is decomposed and denatured by the action of enzymes and radicals, whereby the skin loses elasticity and aging is accelerated.

For example, it is known that by maintaining normal proliferation and differentiation of keratinocytes, appropriate moisture can be retained in the stratum corneum, and fine wrinkles, desquamation, and drying can be prevented and fresh skin can be maintained.

It is known that elastin present in the dermis is decomposed and denatured by the enzyme elastase and radicals, and the elastin is decomposed and denatured, whereby the skin loses elasticity and aging is promoted. .

Further, the present inventors have conducted intensive studies on the aging phenomenon of the skin. As a result, the elastin is present not only in the dermis but also in the epidermis, and the elastin in the epidermis is decomposed by elastase and radicals. This affects the normal keratinization of epidermal cells and causes skin aging, such as firmness, fine wrinkles, dark spots, and dryness.In other words, inhibiting elastase activity suppresses dermal elastin aging. In addition, it has been found that the elastin in the epidermis is also kept normal, and an elastic stratum corneum rich in water retention ability is produced.

[0007]

Therefore, based on the above findings, the present invention can maintain elastic and fresh skin by controlling skin differentiation and proliferation and / or suppressing elastase activity. It is an object to provide a skin external preparation.

[0008]

Means for Solving the Problems The present invention has been made to solve the above-mentioned problems, and the present inventors have developed an oligopeptide consisting of the amino acid sequence Val Gly Val Ala Pro Gly or a polypeptide containing the oligopeptide, The present inventors have found that a protein or an extract maintains normal differentiation and proliferation of keratinocytes, and completed the present invention characterized by comprising the components.

According to an external preparation for skin comprising an oligopeptide having the above amino acid sequence or a protein containing the oligopeptide, an appropriate amount of water can be retained in the stratum corneum, so that elastic and fresh skin is maintained. be able to.

[0010] The present inventor has also proposed that chlorella (genus Chlorella) and ascophyllum (scientific name:
Ascophyllum Nodosum), Milk Thistle (scientific name: SilybummarianumL.),
Snowy grass (scientific name: Cetella asiatica)
It has been found that the extract of (L) Urb) inhibits elastase activity, and has completed the present invention characterized in that one or more selected from the extracts are blended.

With respect to the elastase activity inhibition evaluation on the elastase activity inhibition effect of the extract of the present invention, test methods and test results will be described below.

Test Method The elastase activity inhibition evaluation was carried out according to the method of Bieth J.
(Reference The synthesis and an
everyday use of a highly
Sensitive and convenient
Substrate of elastase. Bio
chem. Med. 1974; 11).

First, each evaluation sample at an optimum concentration was added to PBS and adjusted to a final volume of 100 μl. 100 μl of each sample solution
Succinyl-L-, a synthetic substrate of elastin
(Alanyl) 3-Paranithioanilid
e was added in the same amount under the condition of 50 mM Tris-HCL (pH 7.8) and incubated for 18 hours.

Thereafter, the elastase activity inhibitory effect was measured by the change in absorbance at 410 nm. In addition, the solution to which no sample was added was used as a control.

Test Results Table 1 shows the results of the above test.

[Table 1]

According to the external preparation for skin containing the above extract such as chlorella, the elastin of the epidermis and the dermis can be kept normal, so that the elastic and fresh skin can be kept.

Therefore, the external preparation for skin according to claim 2 is preferably an elastase activity inhibitor.

Furthermore, if the oligopeptide consisting of the amino acid sequence Val Gly Val AlaPro Gly or the protein containing the oligopeptide and the extract of chlorella are used in combination, the differentiation and proliferation of skin Control and elastase activity inhibitory effects are synergistically exerted, and more elastic and fresh skin can be maintained.

Further, the external preparation for skin of the present invention is preferably an anti-aging agent or an external preparation for improving keratinization.

Hereinafter, the configuration of the present invention will be described in detail.

The external preparation for skin of the present invention has an amino acid sequence Va
An oligopeptide consisting of lGlyValAlaProGly is blended as a constituent, or a polypeptide, protein or extract containing the oligopeptide is blended as a constituent. still,
The oligopeptide may be formulated as a salt such as a hydrochloride or an acetate, and may be of any origin as long as it has the amino acid sequence.

The chlorella (genus Chlorella) and ascophyllum (scientific name: Ascophyllum Nodosu) used in the invention of claim 2 are also used.
m), Milk Thistle (scientific name: Silybummaria)
numL. ), Snowy grass (scientific name: Cetella asi)
atica (L) Urb), its leaves,
A stem, flower, bark, seed, fruit, or plant foreground is immersed or heated through an extraction solvent, and then filtered and concentrated.

The extraction solvent is not particularly limited as long as it is a solvent usually used for extraction. An organic solvent such as alcohols such as methanol and ethanol, aqueous alcohols, acetone and ethyl acetate is used alone or in combination. Can be.

In the present invention, the amount of the extract is 0.0005 to 50.0% of the total amount of the external preparation for skin,
% By weight, preferably 0.001 to 10.0% by weight. If the amount is less than 0.0005% by weight, the effects of the present invention are not sufficiently exhibited. If the amount is more than 50.0% by weight, it is difficult to formulate the preparation, which is not preferable.
This is because no significant improvement in the effect is observed even when the content is 0.0% by weight or more.

Further, in addition to the above-mentioned essential components, the skin external preparation of the present invention contains components usually used in skin external preparations such as cosmetics and pharmaceuticals, such as whitening agents, moisturizing agents, antioxidants, oily components, and ultraviolet rays. Absorbents, surfactants, thickeners, alcohols, powder components, coloring materials, aqueous components, water, various nutrients, and the like can be appropriately compounded.

The external preparation for skin of the present invention includes, for example,
Ointments, creams, emulsions, lotions, packs, bath preparations and the like may be used as long as they have been conventionally used as external preparations for the skin, and the dosage form is not particularly limited.

[0027]

EXAMPLES Examples of the present invention will now be described with reference to examples of various formulations. Note that the present invention is not limited to this. Further, the oligopeptide of each example has an amino acid sequence Val Gly Val Ala Pro Gl
2 shows an oligopeptide consisting of y.

Example 1 In this example, a cream was prepared based on the following formulation. In addition, the numerical value of each component shows weight%. Cetanol 2.0 Lipophilic glyceryl monostearate 3.5 Liquid paraffin 10.0 2-Ethylhexyl isononanoate 10.0 Sorbitan monostearate 4.5 Methylpolysiloxane 1.0 N-acyl-L-sodium glutamate 0. 5 1,3-butylene glycol 5.0 concentrated glycerin 10.0 paraben 0.2 oligopeptide hydrochloride 0.5 chlorella extract 0.2 ascophyllum extract 0.5 milk thistle extract 0.1 snowpack extract 0. 3 Remaining purified water

Example 2 In this example, an essence was prepared based on the following formulation. In addition, the numerical value of each component shows weight%. 1,3-butylene glycol 5.0 concentrated glycerin 10.0 citric acid 0.02 sodium citrate 0.1 sodium hyaluronate 0.2 paraben 0.2 acetate of oligopeptide 0.2 chlorella extract 2.0 ascophy Lamb extract 2.0 Milk thistle extract 0.2 Snowpack extract 0.2 Purified water

Example 3 In this example, a lotion was prepared based on the following formulation. In addition, the numerical value of each component shows weight%. Ethanol 5.0 Polyoxyethylene hydrogenated castor oil 0.3 Concentrated glycerin 3.0 1,3-butylene glycol 5.0 Citric acid 0.02 Sodium citrate 0.1 Oligopeptide hydrochloride 0.1 Chlorella extract 1. 0 Ascophyllum extract 1.0 Milk thistle extract 0.1 Snowpack extract 0.1 Purified water

[0031]

As described above, according to the external preparation for skin according to claim 1, by blending a peptide having a specific amino acid sequence, an appropriate amount of water is retained in the stratum corneum,
Keeps elastic and fresh skin, resulting in youthful skin without sagging.

Further, according to the external preparation for skin according to the second aspect, the specific extract is added to inhibit the elastase activity, to keep the elastin of the epidermis and the dermis normal and to have elasticity and freshness. As a result of maintaining the skin, youthful skin without sagging can be created.

Therefore, it is preferable that the external preparation for skin according to claim 2 is an elastase activity inhibitor.

[0034] When the components described in claim 1 and the components described in claim 2 are combined, a more youthful skin is obtained due to a synergistic effect of controlling the differentiation and growth of the skin and suppressing the elastase activity. Can be made.

Accordingly, it is preferable that the skin external preparation of the present invention is an anti-aging agent or an external preparation for improving keratinization.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61P 17/12 A61K 31/00 617G 17/00 617 A61K 35/78 35/78 C 35/80 35/80 A 38/00 37/02 (72) Inventor Tsuyoshi Shigematsu 3-19-3 Toyosaki, Kita-ku, Osaka-shi, Osaka Pears Co., Ltd. (72) Masako Tsutsumi 3-19-3, Toyosaki, Kita-ku, Osaka, Osaka No.3 Pierce Co., Ltd. F-term (reference) 4C083 AA111 AC022 AC072 AC102 AC122 AC242 AC302 AC352 AC422 AC432 AC442 AC482 AC662 AD152 AD332 AD411 AD412 CC02 CC04 CC05 DD27 DD31 EE06 EE07 EE12 EE13 4C084 AA02 BA01 BA02 BA17 DC41 MA22 MA28 MA63 ZC522 4C088 AA16 AB26 AB99 AC01 AC03 AC04 AC05 AC06 AC15 BA08 BA10 BA16 MA07 MA63 NA14 ZA89 ZC20 ZC52

Claims (6)

[Claims] 1. The amino acid sequence Val Gly Val
An external preparation for skin, comprising an oligopeptide comprising Ala Pro Gly or a polypeptide, protein or extract containing the oligopeptide. 2. Chlorella (genus Chlorella), Ascophyllum (scientific name: Ascophyllum Nod)
osum), Milk Thistle (scientific name: Silybumma)
rianummL. ), Snowpack (scientific name: Cetella)
A skin external preparation comprising one or more selected from extracts of asiatica (L) Urb). 3. The method according to claim 2, which is an elastase activity inhibitor.
The topical skin preparation according to the above. 4. The amino acid sequence Val Gly Val
Oligopeptide consisting of Ala Pro Gly or a polypeptide, protein or extract containing the oligopeptide, chlorella (genus Chlorella), ascophyllum (scientific name: Ascophyllum Nodo)
sum), Milk Thistle (scientific name: Silybummar)
ianum mL. ), Snowpack (scientific name: Cetella a)
An external preparation for skin characterized by comprising one or more selected from extracts of siaica (L) Urb). 5. The external preparation for skin according to claim 1, which is an anti-aging agent. 6. The external preparation for skin according to claim 1, which is an external preparation for improving keratinization.