JPH1029924A - Antiaging agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare an antiaging agent, comprising one or more extracts from specific plants blended as active ingredients, having promoting actions, etc., on the production of hyaluronic acid and capable of retaining the tensity or elasticity of skin and maintaining a state of youthful skin. SOLUTION: This antiaging agent is prepared by blending one or more selected from extracts obtained from plants comprising Kayu legi (botanical name: Glycyrrhiza glabra), Kelabet (botanical name: Trigonella foenum-graecum), Lempuyang (botanical name: Zingiber aromaticum Mal.) and Remujung (botanical name: Orthosiphon aristatus) therein. All the plants grow in a dry grassland, a pasture, etc., of Indonesia. The amount of the blended extracts is 0.0005-20.0wt.%, preferably 0.001-10.0wt.% expressed in terms of a dry substance in the total amount of a preparation for external use. The antiaging agent can suitably be used for a makeup cosmetic, a cosmetic for hair of the head, a bathing agent, etc., including a basic cosmetic.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an anti-aging agent,
More specifically, it has a hyaluronic acid production promoting action and the like,
The present invention relates to an anti-aging agent capable of maintaining skin firmness and elasticity and maintaining a youthful skin condition. The anti-aging agent of the present invention can be suitably used for basic cosmetics, makeup cosmetics, hair cosmetics, bath additives, and the like.

[0002]

2. Description of the Related Art Conventionally, the necessity of an anti-aging agent has been considered, but the mechanism and definition of aging have not been clarified. The measurement of the moisturizing state and the measurement of the elasticity of the skin have been performed, and the skin color has been visually observed for judgment. However, in recent years, research on aging has been advanced, and aging is an important factor as a cause of skin aging macroscopically, and furthermore, drying, oxidation, sunlight (ultraviolet rays)
The effects of aging have also been cited as direct factors related to skin aging. Specific phenomena of skin aging include:
It is known that collagen and elastin in skin dermis are reduced, mucopolysaccharides such as hyaluronic acid are reduced, and cells are damaged by ultraviolet rays.

[0003] Among these, hyaluronic acid retains water in the intercellular space, retains cells based on the formation of a jelly-like matrix in tissues, retains lubricity and flexibility of tissues, and has mechanical obstacles. It has many functions such as resistance to external forces and prevention of bacterial infection (BIO IN
DUSTRY, Vol. 8, p. 346, 1991). For example, the hyaluronic acid in the skin decreases with age,
As a result, it is said to cause aging such as wrinkles and rust. As an agent for improving such aged skin,
Many cosmetics containing collagen or hyaluronic acid have been proposed, but they only improve the moisturizing effect on the surface and do not essentially improve aging skin. Others
Although vitamins and herbal medicines are used as skin cell activators, the current situation is that the treatment of aging skin has not yet been achieved.

[0004] Hyaluronic acid in synovial fluid covers the surface of articular cartilage and contributes to smooth operation of joint functions. Hyaluronic acid in normal human joint fluid is about 2.3mg / m
In the case of rheumatoid arthritis, the concentration of hyaluronic acid in the synovial fluid drops to about 1.2 mg / ml, and at the same time the viscosity of the synovial fluid drops significantly (Arthritis®
hematism, 10, 357, 1967
Year). It is also known that a decrease in hyaluronic acid content occurs in purulent arthritis and gouty arthritis as in rheumatoid arthritis [Connective tissue (Kanehara Publishing), 4
81, 1984]. In the above diseases, it is conceivable to increase the amount of hyaluronic acid in synovial fluid in order to improve the lubricating function, cover and protect articular cartilage, suppress pain, and improve the properties of pathological synovial fluid. For example, when rheumatoid arthritis patients undergo joint injection therapy with sodium hyaluronate, the above improvement has been observed (inflammation, vol. 11,
16, 1991). Similarly, in traumatic arthrosis, osteoarthritis and osteoarthritis, the above-mentioned improvement effect has been reported by joint injection therapy of hyaluronic acid [Connective tissue and disease (Kodansha), page 246, 1980]. But,
Treatment of the above diseases is long-term and requires a physician's prescription. Therefore, an ointment or gel containing a hyaluronic acid production promoter which can be easily treated in daily life has been desired. It is also known that during the healing process after burn injury, hyaluronic acid increases significantly in granulation during the period from the initial stage of granulation tissue growing below the necrotic tissue until the entire tissue is replaced by granulation tissue. [Connective tissue and disease (Kodansha), p.153, 1980
, A hyaluronic acid production promoter is also expected as an early treatment for burns.

Drugs that promote the production of hyaluronic acid in human cells include insulin-like growth factor-1 and epidermal growth factor (Biochimica Biophysica Ac).
ta, 1014, p. 305, 1989) and interleukin-1 (Journal of the Japan Society of Obstetrics and Gynecology, 41, 1
943, 1989) or phorbol ester (Experimental C).
cell Research, 148, 377, 19
1983), but none of them can be safely used as cosmetics, bath salts or pharmaceuticals.

[0006]

As a solution to these problems, the present inventors examined the ability of a wide variety of substances to promote the production of hyaluronic acid. The present inventors have found that they have an effect, and have completed the present invention. The plant extract of the present invention is known as a whitening agent, an antioxidant, an anti-inflammatory agent and the like (Japanese Patent Application Nos. 7-200467 and 7-3).
No. 48441, Japanese Patent Application No. 7-191232), and no application to anti-aging agents or hyaluronic acid production promoters is known. The present inventors have completed the present invention based on the above findings.

[0007] That is, the present invention is an anti-aging agent comprising one or more selected from the following plant extracts. (1) Kayu legi (scientific name: Glycyrrhi
za glabra) (2) Kerabet (scientific name: Trigonella foenu)
m-graecum) (3) Lempuyang (scientific name: Zingiber)
aromaticum Mal.) (4) Remujung (scientific name: Orthosipho)
n aristatus) The anti-aging agent of the present invention is preferably a hyaluronic acid production promoter.

Hereinafter, the configuration of the present invention will be described in detail. All of the plant extracts used in the present invention are plants that grow on dry grasslands, pastures and the like in Indonesia. Plant extracts used in the present invention, its leaves, stems, flowers, bark,
The xylem, seed or fruit, whole plant, or the like is obtained by immersing or heating and refluxing with an extraction solvent, followed by filtration and concentration.
The extraction solvent used in the present invention is not particularly limited as long as it is a solvent usually used for extraction. In particular, an organic solvent such as alcohols such as methanol and ethanol, aqueous alcohols, acetone, and ethyl acetate is used alone or in combination. Can be.

The amount of the plant extract in the present invention is as follows:
It is 0.0005 to 20.0% by weight, preferably 0.001 to 10.0% by weight as a dry matter in the total amount of the external preparation.
If the amount is less than 0.0005% by weight, the effects of the present invention cannot be sufficiently exerted, and if it exceeds 20.0% by weight, it is difficult to formulate the composition, which is not preferable. Further, even if the content is 10.0% by weight or more, the effect is not so much improved.

[0010] Since all of the plant extracts used in the present invention have an excellent hyaluronic acid production promoting action on human skin, the anti-aging agent containing the plant extract is effective in improving the skin. It can prevent aging and maintain a youthful and healthy skin condition. Kayu legi can also be used as an elastase inhibitor, and kerabe can be used as a collagen production promoter. Elastase inhibitors suppress the action of elastase, an elastin-disrupting enzyme, and give the skin elasticity and firmness.Collagen production promoters act on fibroblasts in the skin and are one of the important components of the dermis. It promotes the biosynthesis of certain collagen to prevent aging of the skin, and any of them can be used as an anti-aging agent like the hyaluronic acid production promoter.

The anti-aging agent of the present invention includes, in addition to the above essential components, components usually used in external preparations for skin such as cosmetics and pharmaceuticals, for example, whitening agents, moisturizing agents, antioxidants, oily components,
An ultraviolet absorber, a surfactant, a thickener, an alcohol, a powder component, a coloring material, an aqueous component, water, various skin nutrients, and the like can be appropriately compounded as necessary.

In addition, sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, tranexamic acid and derivatives thereof, and licorice extract Products, glabridine, hot water extract of karin fruit, various crude drugs, drugs such as tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, etc. Other whitening agents, sugars such as glucose, fructose, mannose, sucrose, trehalose and the like can also be appropriately compounded.

The present invention can be widely applied to cosmetics, quasi-drugs, etc. applied to the outer skin, and particularly preferably to cosmetics, and its dosage form is also aqueous solution type, solubilizing type, emulsifying type. A wide range of dosage forms can be used, such as powder, oil-liquid, gel, ointment, aerosol, water-oil two-layer systems, water-oil-powder three-layer systems, and the like. In other words, if it is a basic cosmetic, face wash, lotion,
The present invention can be widely applied to various forms such as emulsions, creams, gels, essences (packs, masks) and the like. In addition, if it is makeup cosmetics,
As a toiletry product such as a foundation, it can be widely applied to forms such as a body soap and a soap. Furthermore, quasi-drugs can be widely applied to various ointments and the like. And in these dosage forms and forms,
The form that the anti-aging agent of the present invention can take is not limited.

[0014]

EXAMPLES Next, the anti-aging agent of the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. The compounding amount is% by weight. Prior to the examples, test methods and results of the plant extract of the present invention for promoting hyaluronic acid production, inhibiting elastase, and promoting collagen production will be described.

1. Preparation of Sample (1) Kayu legi extract 50 g of Kayu legi wood was immersed in ethanol for 1 week at room temperature, the extract was concentrated, and 4.5 g of ethanol extract was obtained. Obtained. This extract was dissolved in DMSO at 1%, the concentration was adjusted by diluting this solution, and the following experiment was performed using this.

(2) Kelavet Extract 50 g of Kelavet seeds were immersed in ethanol at room temperature for 1 week, and the extract was concentrated to obtain 0.05 g of ethanol extract. This extract was added to DMSO for 1
%, And the concentration was adjusted by diluting this solution. Using this, the following experiment was performed.

(3) Extract of Lempuyan Extract 50 g of rhizome of Lempuyan
Was immersed in ethanol at room temperature for 1 week, and the extract was concentrated to obtain 2.5 g of an ethanol extract. This extract is
After dissolving 1% in MSO and diluting this solution to adjust the concentration, the following experiment was performed using this.

(4) Remujung extract 50 g of whole plant of Remujung is
The extract was immersed in ethanol at room temperature for 1 week, and the extract was concentrated to obtain 5.5 g of an ethanol extract. This extract is combined with DMSO
And the concentration was adjusted by diluting this solution, and the following experiment was performed using this.

2. Test method and results Hyaluronic acid production promoting effect Human skin fibroblasts were spread 20,000 in a 96-well petri dish, and 48
After culturing in RITC80-7 containing 10% FBS for a time, the medium was replaced with a medium containing 0.5% FBS, a plant extract dissolved in DMSO was added, and the cells were further cultured for 48 hours.
DMSO should be 1/200 (5 μl per 1 ml of medium)
l) Added. The extract concentration was 10 ⁻⁵ to 10 ⁻² wt%. After the culture, the medium was collected and used for the measurement of hyaluronic acid. Further, the amount of DNA in the petri dish was measured and used as an index of the cell number. The amount of DNA was measured by a fluorescence measurement method using H33258. In the Kayu-Reggi extract, the Kerabe extract and the Lemjan extract, cytotoxicity was observed at 10 ^-2 % by weight, but no toxicity was observed at 10 ^-3 % by weight. In the case of the Lumpuyan extract, cytotoxicity was observed at 10 ^-3 % by weight, but no toxicity was observed at 10 ^-4 % by weight. Therefore, when the amount of hyaluronic acid per DNA of the sample to which no plant extract was added (control) was 100, the DNA of the sample to which plant extract was added at the maximum concentration at which no toxicity was observed for each plant extract
The amount of hyaluronic acid per unit was measured using a hyaluronic acid measurement kit (Chugai Pharmaceutical), and the result was defined as the hyaluronic acid production promotion rate (%). Table 1 shows the results. In addition, as a reference example, the same test as described above was conducted on a ginseng solvent extract already known to have a hyaluronic acid production promoting effect. Table 1 also shows the results.

[0020]

[Table 1] 効果 Hyaluronic acid production promoting effect (%) test ──────────── ─── 10 ^-3 10 ^-4 ───────────────────────── Kayu / Legi extract 145 − Kerabe extract 194 − Lumpuyan extract Toxicity 162 Lemjan extract 130 − Ginseng extract 130 − ──────────────────────────

Elastase Inhibitory Activity Elastase activity was measured as follows according to the method of Fujie et al. As a reaction buffer, 0.1 M
This was performed using HEPES, 0.5 M NaCl (pH 7.4). Methoxy-succinyl as an elastase substrate
-alanyl-alanyl-prolyl-valine-p-nitroanilide (BA
CHEMFEINCHEMIKALIENAG) to 8
It was dissolved in DMSO to 0 mM, dispensed in 20 μl aliquots, and stored frozen (−80 ° C.). At the time of use, it was used after being diluted to 8 mM with a reaction buffer. Elastase is elastase derived from human leukocytes (ELASTI).
N PRODUCT CO., INC.)
Dissolve in the reaction buffer to a concentration of 10 μg / ml.
Each was dispensed and stored frozen (-80 ° C). In use,
The solution was diluted to 5 μg / ml with a reaction buffer and used. 25 μl each of 8 mM elastase substrate was dispensed into a 96-well plate (CORNING 25860), and 50 μl of the inhibitor (the above 1% sample solution was diluted with a reaction buffer to adjust the concentration to 100 ppm). ) Was added. Next, 25 μl of 5 μg / ml elastase was added on ice, and the mixture was immediately incubated at 37 ° C. for 20 minutes. Thereafter, the absorbance was measured at 415 nm. However, the inhibition rate is based on the following function.

[0022]

## EQU1 ## Inhibition rate (%) = 100− (in the presence of inhibitor / no inhibitor) × 100

The results are shown in Table 2. In addition, as a reference example, a test similar to the above was performed on a soybean extract (trade name: Elhibin; manufactured by Pentafarm) already known to have elastase inhibitory activity. Table 2 also shows the results.

[0024]

[Table 2] ────────────────────────── Elastase inhibition rate (%) ────────────── ──────────── Kayu leggi extract 90.6 Soybean extract 84.2 ──────────── ──

Collagen production-promoting action Human skin fibroblasts were seeded in a 96-well petri dish by 20,000, and
After culturing in RITC80-7 containing 10% FBS for a time, the medium was replaced with a medium containing 0.5% FBS, a plant extract dissolved in DMSO was added, and the cells were further cultured for 48 hours.
DMSO should be 1/200 (5 μl per 1 ml of medium)
l) Added. The extract concentration was 10 ⁻⁵ to 10 ⁻² wt%. After the culture, the medium was collected and used for collagen measurement.
Further, the amount of DNA in the petri dish was measured and used as an index of the cell number. The amount of DNA was measured by a fluorescence measurement method using H33258. With respect to the Kerabe extract, cytotoxicity was observed at 10 ^-2 % by weight, but no toxicity was observed at 10 ^-3 % by weight. Therefore, type I procollagen C-terminal peptide (Procollage) produced by cultured human dermal fibroblasts
n type I carboxyterminal propeptide (PIP)
When the amount of PIP per DNA of the sample (control) to which no plant extract was added, as measured by the SA method, was taken as 100, the P of the plant extract-added sample having a concentration of ^10-3 % by weight was calculated.
The IP amount was measured, and the result was defined as the collagen production promotion rate (%).
Table 3 shows the results. In addition, as a reference example, the same test as described above was performed on a solvent extract of Rishimi, which is already known to have a collagen production promoting action. Table 3 also shows the results.

[0026]

[Table 3] ───────────────────────────── Test Collagen production promoting effect (%) ───────── << Kerabe extract 197.6 Rishimi extract 124.1 >> ────────────

Examples of the formulation of the anti-aging agent according to the present invention in various dosage forms are described below as examples.

Example 1 Cream (Formulation) Stearic acid 5.0% by weight Stearyl alcohol 4.0 Isopropyl myristate 18.0 Glycerin monostearate 3.0 Propylene glycol 10.0 Kayu legi methanol extract 0.01 Caustic potash 0.2 Sodium bisulfite 0.01 Preservatives Appropriate amount Fragrance Appropriate amount Ion-exchange water residue (Production method) Propylene glycol and kayu in ion-exchange water
The legi methanol extract and caustic potassium are added and dissolved, and the mixture is heated and maintained at 70 ° C (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the water phase, and after the addition is completed, the temperature is maintained for a while to cause a reaction. Thereafter, the mixture is uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well.

Example 2 Cream (Formulation) Stearic acid 2.0% by weight Stearyl alcohol 7.0 Hydrogenated lanolin 2.0 Squalane 5.0 2-octyldodecyl alcohol 6.0 Polyoxyethylene (25 mol) cetyl alcohol ether 3.0 Glycerin monostearate 2.0 Propylene glycol 5.0 Kerabe ethanol extract 0.05 Sodium bisulfite 0.03 Ethyl paraben 0.3 Perfume Appropriate amount Ion-exchanged water Residue And add
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase, pre-emulsified, homogenized with a homomixer, and cooled to 30 ° C. with good stirring.

Example 3 Cream (Formulation) Solid paraffin 5.0% by weight Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) sorbitan monolaurate 2 0.0 Soap powder 0.1 Borax 0.2 Lumpuyan acetone extract 0.05 Remjan ethanol extract 0.05 Sodium bisulfite 0.03 Ethyl paraben 0.3 Perfume Appropriate amount Ion exchange water Residue (Production method) Ion exchange Soap powder and borax are added to water, heated and dissolved, and kept at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture is uniformly emulsified with a homomixer, and cooled to 30 ° C. while stirring well after emulsification.

Example 4 Emulsion (Formulation) 2.5% by weight of stearic acid Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 0 Triethanolamine 1.0 Carboxyvinyl polymer 0.05 (Product name: Carbopol 941, BFGoodrich Chemical company) Kayu-Reggi acetic acid ethyl ester extract 0.01 Sodium bisulfite 0.01 Ethyl paraben 0.3 Fragrance Appropriate ion Exchange water residue (Preparation method) Dissolve the carboxyvinyl polymer in a small amount of ion exchange water (phase A). Polyethylene glycol 1500 and triethanolamine are added to the remaining ion-exchanged water, dissolved by heating, and kept at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase to perform preliminary emulsification, the phase A is added, and the mixture is uniformly emulsified with a homomixer. After the emulsification, the mixture is cooled to 30 ° C. while stirring well.

Example 5 Emulsion (Formulation) Microcrystalline wax 1.0% by weight Beeswax 2.0 Lanolin 20.0 Liquid paraffin 10.0 Squalane 5.0 Sorbitan sesquioleate 4.0 Polyoxyethylene (20 mol) ) Sorbitan monooleate 1.0 Propylene glycol 7.0 Kerabeacetone extract 10.0 Sodium bisulfite 0.01 Ethylparaben 0.3 Appropriate amount Ion-exchanged water Residue (Preparation method) Add propylene glycol to ion-exchanged water,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase is gradually added thereto, and the mixture is uniformly emulsified with a homomixer. After emulsification, cool to 30 ° C with good stirring.

Example 6 Jelly (Formulation) 95% ethyl alcohol 10.0% by weight Dipropylene glycol 15.0 Polyoxyethylene (50 mol) oleyl alcohol ether 2.0 Carboxyvinyl polymer 1.0 (Product name: Carbopol) 940, BFGoodrich Chemical company) Caustic soda 0.15 L-arginine 0.1 Lumpuyan 50% aqueous ethanol extract 7.0 Sodium 2-hydroxy-4-methoxybenzophenonesulfonate 0.05 Ethylenediaminetetraacetate trisodium 2 water 0 .05 Methyl paraben 0.2 Perfume Appropriate amount Ion-exchanged water Residue (Preparation method) Carbopol 940 was uniformly dissolved in ion-exchanged water, while 95% ethanol was extracted from a 50% aqueous solution of lumpuyan ethanol, polyoxyethylene (50 mol)
Dissolve the oleyl alcohol ether and add to the aqueous phase. Then, after adding other components, caustic soda, L
-Neutralize with arginine to thicken.

Example 7 Serum (Prescription) (Phase A) Ethyl alcohol (95%) 10.0% by weight Polyoxyethylene (20 mol) octyldodecanol 1.0 Pantothenyl ethyl ether 0.1 Lemjan methanol extraction Product 1.5 Methyl paraben 0.15 (B phase) Potassium hydroxide 0.1 (C phase) Glycerin 5.0 Dipropylene glycol 10.0 Sodium bisulfite 0.03 Carboxyvinyl polymer 0.2 (Product name: Carbopol) 940, BFGoodrich Chemical company) Purified water residue (Preparation method) Dissolve A phase and C phase uniformly, and add A to C phase
Add phases and solubilize. Next, after adding the phase B, filling is performed.

Example 8 Pack (Formulation) (A phase) Dipropylene glycol 5.0% by weight Polyoxyethylene (60 mol) hydrogenated castor oil 5.0 (Phase B) Kayu / Legi methanol extract 0.01 Olive oil 5 2.0 Tocopherol acetate 0.2 Ethyl paraben 0.2 Fragrance 0.2 (Phase C) Sodium bisulfite 0.03 Polyvinyl alcohol 13.0 (Saponification degree 90, Degree of polymerization 2,000) Ethanol 7.0 Purified water Residue (Production method) A phase, B phase, and C phase are each dissolved uniformly,
Add phase B to phase and solubilize. Next, this is added to the C phase and then filled.

Example 9 Solid foundation (formulation) Talc 43.1% by weight Kaolin 15.0 Sericite 10.0 Zinc white 7.0 Titanium dioxide 3.8 Yellow iron oxide 2.9 Black iron oxide 0.2 Squalane 8 0.0 Isostearic acid 4.0 POE sorbitan monooleate 3.0 Isocetyl octoate 2.0 Kerabetanol extract 1.0 Preservatives Appropriate amount Fragrance Appropriate amount (Preparation method) Powdery components of talc to black iron oxide are sufficiently mixed in a blender. Then, the oily components of squalane to isocetyl octanoate, kerave ethanol extract, preservative, and fragrance are added, kneaded well, and the mixture is filled into a container and molded.

Example 10 Emulsified Foundation (Cream Type) (Prescription) (Powder) Titanium Dioxide 10.3% by weight Sericite 5.4 Kaolin 3.0 Yellow Iron Oxide 0.8 Bengala 0.3 Black Iron Oxide 0.2 (oil phase) Decamethylcyclopentasiloxane 11.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.0 (aqueous phase) Purified water 50.0 1,3-butylene glycol 4.5 Lum Puyang ethanol extract 1.5 Sorbitan sesquioleate 3.0 Preservatives Appropriate amount Flavors Appropriate amount (Preparation method) After heating and stirring the aqueous phase, a well-mixed and pulverized powder portion is added, followed by homomixer treatment. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

[0038]

As described above, according to the present invention,
By exerting the effect of promoting hyaluronic acid production, it suppresses the denaturation of elastin, which is an elastic fiber, and can maintain elastic, wrinkle-free and sagging skin. The present invention provides an anti-aging agent having an excellent cosmetic effect, such as maintaining the condition of the skin. In addition, some of the anti-aging agent of the present invention suppresses the activity of elastase,
It also has the effect of promoting the production of collagen, can more strongly prevent aging of the skin, and can maintain a youthful skin condition.

Continuation of the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display location A61K 35/78 AGZ A61K 35/78 AGZC (72) Inventor Masahiro Ota 1050 Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa Shiseido Daiichi Research Center

Claims (3)

[Claims] 1. An anti-aging agent comprising one or more selected from the following plant extracts. (1) Kayu legi (scientific name: Glycyrrhi
za glabra) (2) Kerabet (scientific name: Trigonella foenu)
m-graecum) (3) Lempuyang (scientific name: Zingiber)
aromaticum Mal.) (4) Remujung (scientific name: Orthosipho)
n aristatus) 2. The method according to claim 1, which is a hyaluronic acid production promoter.
The anti-aging agent according to the above. 3. The anti-aging agent according to claim 1, which is an anti-aging cosmetic.