JP2015151390A - Humectant, antiinflammatory agent, antioxidant, skin turnover improver, cell activator and skin-whitening agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional material that is derived from natural products and has excellent biological safety, and is blended in cosmetics (including quasi drugs) or cosmetic oral compositions.SOLUTION: This invention provides a humectant, an antiinflammatory agent, an antioxidant, a skin turnover improver, a cell activator and a skin-whitening agent, comprising an extract obtained from an immature fruit of Prunus persica, belonging to the genus Prunus of Rosaceae, as an active ingredient, and a cosmetic or a cosmetic oral composition obtained by blending them.

Description

  The present invention relates to a functional material excellent in biological safety and blended in cosmetics (including quasi-drugs) and oral cosmetic compositions.

  Skin aging is caused by internal factors such as inactivation of cell growth / differentiation, hormonal secretion, and quantitative reduction of extracellular matrix components with aging, active oxygen induced by sunlight (ultraviolet rays), chemistry It is a phenomenon that is caused by intricately intertwining substances or external factors such as environmental stress. Among them, ultraviolet rays and chemical substances, which are external factors, may damage skin cells and tissues, alter biological components, and as a result, may cause generation of antigens in the skin. Therefore, these external factors can cause skin inflammation and allergies due to antigens, and also cause abnormal deposition of melanin pigments to cause spots, buckwheat, liver spots, etc. Will cause damage.

  In order to prevent skin aging caused by the above factors and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetic and oral compositions containing these active ingredients have been proposed. Is on the market. For example, antioxidants such as vitamin Cs and vitamins E; anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; cell activation such as α-hydroxycarboxylic acid, placenta extract, γ-amino-β-hydroxybutyric acid Ingredients: extracellular matrix components such as collagen, elastin and hyaluronic acid; humectants such as urea have been proposed. However, it is difficult for conventional active ingredients to sufficiently satisfy both effectiveness and biosafety, and there is a demand for functional raw materials with improved such points.

  In view of the problems of the prior art, the present inventors have conducted intensive research to find a new active ingredient derived from a natural product from the viewpoint of biological safety. As a result, the extract of peach immature fruit, a plant of the genus Rosaceae (peach) genus, exhibits excellent skin beautifying and whitening effects, and is excellent in biological safety and effectiveness by blending the extract. The present inventors have found that it is possible to provide cosmetics and cosmetic oral compositions.

In the cultivation process of peaches, which are Rosaceae plants, in order to adjust the number and size of fruits, an operation of thinning out immature fruits, that is, an operation called “plucking” is performed. For this reason, it has been required to effectively use these thinned immature fruits among peaches.

  Conventionally, cosmetics containing, as an active ingredient, peach seeds (peach seeds) that are plants of the genus Rosaceae (peach) are known (Patent Documents 1 and 2). Moreover, it is known that immature peach fruit has an elastase activity inhibitory action (Patent Document 3). Furthermore, compositions for oral or external use containing polyphenols derived from immature fruits of rose family plants (apples, pears, peaches) as active ingredients are also known (Patent Documents 4 and 5). However, peach immature fruit extract itself has moisturizing action, anti-inflammatory action, antioxidant action, skin turnover improving action, cell activation action and whitening action. Nothing has been known about providing cosmetics and cosmetic oral compositions that are excellent in nature.

JP 11-335235 A JP 07-309770 A JP 2011-105693 JP 08-259453 JP 09-175982

The present invention is a moisturizing agent containing as an active ingredient an extract of an immature fruit of the genus Rosaceae.
Moreover, this invention is an anti-inflammatory agent which uses the extract of the immature fruit of the peach of the Rosaceae cherry genus as an active ingredient.
Moreover, this invention is an antioxidant which uses the extract of the immature fruit of the peach of the Rosaceae cherry genus as an active ingredient.
Moreover, this invention is a skin turnover improving agent which uses the extract of the immature fruit of the peach of the Rosaceae cherry genus as an active ingredient.
Moreover, this invention is a cell activator which uses the extract of the immature fruit of the peach of the Rosaceae cherry genus as an active ingredient.
Moreover, this invention is a whitening agent which uses the extract of the immature fruit of the peach of the Rosaceae cherry genus as an active ingredient.

  According to the present invention, the extract of peach immature fruit, which is an active ingredient, is superior due to the synergistic effect of moisturizing action, anti-inflammatory action, antioxidant action, skin turnover improving action, cell activation action and whitening action. In addition, it is possible to provide a preparation for cosmetics and cosmetic oral compositions. In addition, since the extract is derived from a natural product, it can also provide a cosmetic or cosmetic oral composition excellent in biological safety.

Hereinafter, the present invention will be described in detail. In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.
The varieties of Prunus persica used in the present invention are not particularly limited. Asama white peach, any varieties of peaches may be used.

  The peach immature fruit referred to in the present invention refers to a fruit having a diameter of 0.5 to 5 cm, preferably 2 to 3 cm.

  The extract is prepared by washing the immature fruits of peaches in advance with water if necessary to remove foreign substances, or after drying as it is or after drying, chopping or crushing as necessary, and extracting according to conventional methods such as dipping methods. It can be performed by contacting with a solvent.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Of these extraction solvents, hydrophilic solvents such as water, lower alcohols and polyhydric alcohols are preferred in the present invention. Preferable examples in the case of using this hydrophilic solvent include, for example, water or lower alcohols (especially ethanol) used alone, a mixed solvent of water and lower alcohols (especially ethanol), or water and polyhydric alcohols ( In particular, use of a mixed solvent with 1,3-butylene glycol or propylene glycol) and the like are exemplified, and water alone is most preferable. The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 in a volume ratio (hereinafter the same) if the mixed solvent is water and ethanol, and 1: 1 if the mixed solvent is water and glycerin. If it is -20: 1 and it is a mixed solvent of water and 1,3-butylene glycol, it is preferable to set it as the range of 1: 1 to 20: 1.

  In preparing the extract, there is no particular limitation on the pH of the extract, but generally it is preferably in the range of 4-8. In this sense, if necessary, an alkaline adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide may be blended in the extraction solvent and adjusted to a desired pH.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, when water is used as the extraction solvent, the extraction temperature is preferably in the range of 0 to 85 ° C., and the extraction time Is preferably in the range of 2-3 hours.

  The extract obtained under the above conditions is generally adjusted to a pH of 4 to 8, and even if it is used as it is as a compounding agent for cosmetics or oral cosmetic compositions, it is used as a desired concentration by vacuum concentration or the like. You may do it.

  Since the extract prepared under the above conditions is substantially free of polyphenols, polyphenols derived from immature fruits of the Rosaceae plants (apples, pears, peaches, etc.) disclosed in Patent Documents 4 and 5 above. Distinguished from

  Examples of cosmetics (including quasi-drugs) containing the peach immature fruit extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeups. Makeup cosmetics such as up-pressed powder, blusher, white powder, cleansing cosmetics such as face wash, body shampoo, soap, hair cosmetics such as shampoo, hair conditioner, hair cream, and bath preparations Of course, the present invention is not limited to these. In addition, as cosmetic oral compositions, beverages such as beauty drinks, energy drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products, jelly, candy , Tablets, gums and other foods; tablets, liquids, granules or jelly-like health foods / beverages, etc., but the present invention is not limited to these, and can be incorporated into foods and drinks that can be taken orally can do.

The amount of extract of peach immature fruit in cosmetics is generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% in the case of basic cosmetics, as the solid content of the extract. In the range of% by weight, in the case of makeup cosmetics, it is generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. The range is 002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0 wt% (solid content wt%, the same shall apply hereinafter), preferably 0.0001 to 3 0.0% by weight. Moreover, the blending amount of the peach immature fruit extract in the cosmetic oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

  In the cosmetic or cosmetic oral composition, in addition to the essential peach immature fruit extract, components commonly used in cosmetics, such as oily components, surfactants (synthetic and natural products), Moisturizers, thickeners, antiseptics / bactericides, powder components, ultraviolet absorbers, antioxidants, pigments, fragrances and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the peach immature fruit extract are not impaired, there may be no combination of other physiologically active ingredients.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

Examples of emulsifiers and emulsifiers include stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.) It can also be blended.

Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

Examples of the thickener include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white) extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; Examples include glutamic acid and its derivatives.

Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid And derivatives thereof, vitamin E and derivatives thereof, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate). These may be used alone or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, ascorbic acid sugar derivatives such as L-ascorbic acid-5-glucoside, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is Hexanoyl group, o L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid- 2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acylated derivatives thereof, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid As hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as tranexamic acid derivatives, tranexamic acid ester ( For example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, tranexamic acid methylamide) and the like, and resorcinol derivatives include, for example, 4-n- Butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid derivatives such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide and benzyl nicotinate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

As a physiologically active ingredient, as a whitening ingredient, for example, placenta extract, Sakuhakuhi extract, yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, white coconut Fermented product, peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract, pearl hydrolyzed product, pearl seed fermented product, royal jelly Fermented products, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. Coral grass extract as an anti-aging component , Rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or water content thereof Dissolution, apricot fruit extract, seaweed extract such as catamen giraffe, sea flower extract such as sea eel, soy milk fermented product, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germination Rice extract or hydrolyzate thereof, germinated rice fermented product, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot extract, linoleic acid and derivatives or processed products thereof (eg, liposomalization) Linoleic acid, etc.), animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, aloe extract There are extracts, honey comb extracts, loofah extracts, anaosa extracts, Zizyphus joazeiro extracts, and the like.

  Next, the present invention will be described more specifically with reference to production examples, examples (formulation examples), and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of immature fruit extract of peach 60 g of immature fruit of peach (Prunus persica Batsch) was mixed with 600 g of purified water and left standing at 80 ° C. for 2 hours to obtain 450.1 g of extract solution. Got. Thereafter, the obtained extract solution is filtered, and further, 1% activated carbon (manufactured by Wako Pure Chemical Industries, Ltd.) is added to the filtered solution, and the activated carbon treatment is performed for 1 hour. 431.0 g of a fruit extract solution was obtained (pH 4.1, solid content concentration 3.50%).

Comparative Production Example 1 Preparation of peach mature fruit extract 15g of peach (Prunus persica Batsch) mature fruit (dried with skin and seeds) was mixed with 150g of purified water and extracted at 80 ° C for 2 hours. It was. Then, 110.5 g of an extract solution of peach mature fruits was obtained. Thereafter, the obtained extract solution is filtered, and further, 1% activated carbon (manufactured by Wako Pure Chemical Industries, Ltd.) is added to the filtered solution and subjected to activated carbon treatment for 1 hour, and an extract of mature peach fruits is obtained. A solution was obtained (pH 5.0, solid concentration 7.30%).

Example 1. Cream [Component A] Liquid paraffin 5.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Phenoxyethanol 0.1
[Component B] Extract solution of Part Production Example 1 2.5
Glycerin 5.0
Carboxymethyl monostearate 0.1
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Emulsion [Component A] Part Liquid paraffin 6.0
Olive oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
[Component B] Part Extract solution of Production Example 1 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Collagen 0.1
Amount of purified water totaling 100 parts
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 3 Lotion [Component A] Extract solution of part production example 1 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Phenoxyethanol 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Fragrance Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Example 4 Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
[Component B] Extract solution of Part Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[Component C] Part Fragrance Appropriate amounts of component A and component B were each heated to 80 ° C. or higher, component B was added to component A, and the mixture was stirred and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 5 FIG. Emulsion [Component A] Part Liquid paraffin 6.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Production Example 6 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 5. Obtained an emulsion in the same manner as in Example.

Example 7 Emulsion Emulsion in the same manner as in Example 5 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used in the component B of Example 5 instead of 2.0 parts of tranexamic acid. Got.

Example 8 FIG. Emulsion In the component B of Example 5, the emulsion was prepared in the same manner as in Example 5 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 9 Emulsion [Component A] Part Liquid paraffin 6.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 10 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract solution of Production Example 1 2.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 11 Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[Component B] Part Extract solution of Production Example 1 2.5
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D] part fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 12 Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[Component B] Part Extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 13 Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Amounts of 100 parts in total A and B components are each heated to 80 ° C to dissolve uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a hair shampoo It was.

Example 14 Hair conditioner [component A] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water Amounts of 100 parts in total A and B components were each heated to 80 ° C. and uniformly dissolved, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Formulation Example 15. Beauty beverage Extract of Production Example 1 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 16. Extract of tablet production example 1 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After the above-mentioned parts by weight of each component were mixed, they were pressure-molded to obtain tablets.

Test Example 1 Gene expression evaluation test of normal human epidermal cells In this test, the following test was conducted to evaluate the effect of peach immature fruit extract according to the present invention on gene expression of normal human epidermal cells. .
[Test method]
Normal human epidermis cells were prepared to 6 × 10 ⁵ cells / mL with HuMediaKG2 (manufactured by Kurabo Industries Inc.) containing growth additives, 1 mL was seeded in a 6 cm Petri dish, and cultured at 37 ° C. under 5% CO ₂ and saturated steam. did. After culturing for 24 hours, the culture solution containing the extract (sample solution) of Production Example 1 (with the extract added to a final concentration of 2.0% as a solution with respect to the total amount of the culture solution) Was added and cultured. As a comparative control, a control group was set in which a culture solution containing only a 2.0% PBS (−) solution was added instead of the sample solution. After culturing for 24 hours, the cells in each test group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, mixed by stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes using a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, stirred and mixed, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to total RNA, stirred and washed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser [Perfect Real Time] (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is expressed in a certain amount in common in many tissues and cells, and is always expressed and essential for cell maintenance and proliferation) Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of each gene in each test section was compared when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of each gene in the test group when the expression level of each gene in the control group was set to 100 was determined. Test results are shown in Tables 1-6.

[Table 1]

[Table 2]

As shown in Tables 1 and 2, the extract of Production Example 1 according to the present invention is a gene encoding hyaluronoglucosaminidase1 [HYAL1], Hyaluronoglucosaminidase2 [HYAL2], which is an enzyme that degrades hyaluronic acid in epidermal cells. Since the expression of is suppressed, the moisturizing function of the skin can be improved.

[Table 3]

[Table 4]

As shown in Tables 3 and 4, the extract of Production Example 1 according to the present invention is a matrix metalloproteinase (matrix metalloproteinase 2 [MMP2], matrix metalloproteinase 9) that is an enzyme that degrades type IV collagen constituting the basement membrane of epidermal cells. By suppressing the expression of the gene encoding [MMP9]), it is possible to protect the basement membrane of epidermal cells and improve the turnover of the skin surface layer.

[Table 5]

  As shown in Table 5, the extract of Production Example 1 according to the present invention suppresses the expression of the gene encoding interleukin-1 (Interleulin1 alpha [IL1A2]), which is an inflammatory cytokine. And pigmentation can be suppressed.

[Table 6]

As shown in Table 6, the extract of Production Example 1 according to the present invention shows expression of a gene encoding nitric oxide (NO) synthase [NOS2], which is one of the reactive oxygen species. Since it suppresses, the oxidative damage of a cell can be suppressed, and also the skin pigmentation caused by active oxygen can be suppressed.

Test Example 2 Normal human fibroblast gene expression evaluation test In this test, the following test was conducted to evaluate the effect of the extract of peach immature fruit according to the present invention on gene expression of normal human fibroblasts. went.
[Test method]
Normal human fibroblasts were prepared at 6 × 10 ⁵ cells / mL with 0.5% NCS-containing Eagle MEM (manufactured by Nissui Pharmaceutical Co., Ltd.), seeded with 1 mL in a φ6 cm dish, and saturated with 5% CO ₂ . Culturing was performed at 37 ° C. under water vapor. After culturing for 24 hours, the culture solution containing the extract of Production Example 1 according to the present invention (added with the extract so that the final concentration is 2.0% as a solution with respect to the total amount of the culture solution) Was added and cultured. As a comparative control, instead of the extract of Production Example 1 according to the present invention, a culture solution containing only PBS (−) (the final concentration of PBS (−) with respect to the total amount of the culture solution was adjusted to 2.0%). A test group (control group) was added. After culturing for 24 hours, the cells in each test group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, mixed by stirring, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes using a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, stirred and mixed, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to total RNA, stirred and washed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is expressed in a certain amount in common in many tissues and cells, and is always expressed and essential for cell maintenance and proliferation) Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of each gene in each test section was compared when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of each gene in the test group when the expression level of each gene in the control group was set to 100 was determined. Test results are shown in Tables 7-15.

[Table 7]

As shown in Table 7, the extract of Production Example 1 according to the present invention enhances the expression of the gene encoding hyaluronan synthase 2 [HAS2], which is a synthase of dermal hyaluronic acid. The elasticity and the like can be improved.

[Table 8]

As shown in Table 8, the extract of Production Example 1 according to the present invention enhances the expression of the gene encoding the dermal elastin fiber constituent protein (Elastin microfibril interfacer1 [EMILIN1]). Can be improved.

[Table 9]

As shown in Table 9, the extract of Production Example 1 according to the present invention is a gene encoding type XVIII collagen (collagen, type XVIII, alpha 1 [COL18A1]), which is a kind of proteoglycan constituting the basement membrane of the skin. Therefore, synthesis of proteoglycan constituting the basement membrane can be promoted, and skin turnover and the like can be improved.

[Table 10]

  As shown in Table 10, the extract of Production Example 1 according to the present invention suppresses the expression of a gene encoding an enzyme (prostaglandin I2 synthase [PTGIS]) involved in the synthesis of prostaglandin I2, which is an inflammatory substance. Therefore, skin inflammation and pigmentation can be suppressed.

[Table 11]

[Table 12]

As shown in Tables 11 and 12, the extract of Production Example 1 according to the present invention is a glutathione peroxidase (detoxifying harmful molecules in the presence of glutathione, an antioxidant substance in vivo, and involved in oxidative stress response). glutathione peroxidase1 [GPX]) and glutathione-S-transferase mu2 [GSTM2], which enhances the expression of genes, preventing and improving in vivo detoxification effects, oxidative stress disorders and diseases can do.

[Table 13]

[Table 14]

As shown in Tables 13 and 14, to enhance the expression of genes encoding aconitase (Aconitase1 [ACO1], Aconitase2 [ACO2]), enzymes that play an important role in the energy metabolism pathway (TCA circuit) in vivo. Therefore, it is possible to activate intracellular energy synthesis that decreases with aging.

[Table 15]

As shown in Table 15, expression of a gene encoding an inhibitor of metallopeptidase (Metallopeptidase inhibitor3 [TIMP3]), which is an enzyme that degrades extracellular matrix (eg, collagen, proteoglycan, elastin, etc.) is enhanced. The extracellular matrix can be protected.

Test Example 3 Fibroblast ATP synthesis enhancement effect Human dermis-derived fibroblasts NB1RGB were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium (manufactured by Nissui Pharmaceutical). After pre-incubation for 1 day under the conditions of 37 ° C. and 5.0% CO ₂ , the extract of Production Example 1 was used as a sample solution in the medium at a concentration of 2.5% Concentration), and further cultured for 3 days under the same conditions. For comparison, PBS (-) and mature peach extract were also added to a concentration of 2.5% and cultured in the same manner. Next, the medium was removed, PBS (−) was added at 100 μL / well, ATP luminescence reagent (Toyo Orbit) was added at 100 μL / hole, stirred for 1 minute with a microplate shaker, and then set at 23 ° C. (TECAN) was allowed to stand for 10 minutes and the amount of luminescence was measured. In this test system, the relative value of the light emission amount in other test groups when the light emission amount in the PBS (-) 2.5% addition group was set to 100 was determined as the ATP synthesis rate.

The results of Test Example 3 are shown in Table 16.
[Table 16]

As shown in Table 16, it was confirmed that the extract of Production Example 1 according to the present invention has a markedly superior fibroblast ATP synthesis enhancing effect as compared to the extract of peach mature fruit of Comparative Example 1. It was done.

Test Example 4: Confirmation test for phenols (polyphenol, etc.) [Sample]
Extract solution of immature fruit of peach of Production Example 1 (Sample 1 of the present invention)
[Test method]
First, 9 g of ferric chloride (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in 100 mL of purified water to prepare a ferric chloride solution. Next, the ferric chloride solution was added to 1 g of the sample solution, and the coloration was observed.
[result]
Even when a ferric chloride solution was added to Sample 1 of the present invention, no change was observed in the color of the sample solution, and no color reaction was observed. Ferric chloride reacts with phenols (polyphenol, etc.) to show green to black-blue color, so it is confirmed that the extract of peach immature fruit according to the present invention does not contain phenols. It was.

Claims (6)

  A moisturizing agent containing as an active ingredient an extract of immature fruit of Rosaceae Prunus peach (Prunus persica). An anti-inflammatory agent comprising an extract of an immature fruit of Rosaceae (Prunus persica) peach (Prunus persica) as an active ingredient. Antioxidant containing as an active ingredient an extract of immature fruits of Rosaceae (Prunus) peach (Prunus persica). An agent for improving skin turnover comprising an extract of immature fruit of Rosaceae (Prunus persica) peach (Prunus persica) as an active ingredient. A cell activator comprising, as an active ingredient, an extract of an immature fruit of Rosaceae (Prunus) peach (Prunus persica). A whitening agent containing as an active ingredient an extract of immature fruit of Rosaceae (Prunus) peach (Prunus persica).