JP6677446B2 - Cosmetics - Google Patents

Description

  An object of the present invention is to provide a functional material obtained from a plant of the family Rosaceae and having excellent cell soundness and antiaging effects.

  In recent years, research has been conducted on cell aging and damage caused by external factors (for example, ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergic substances such as pollen, environmental stress, etc.), and various cell aging phenomena. And the mechanisms involved in cell damage and repair have been elucidated.

  For example, glycation of proteins in cells is known as a factor of cell aging, and the accumulation of protein glycation end products called advanced glycation end products (AGEs) generated by the reaction is known. Attracting attention. When this protein saccharification reaction occurs in cells, the functions of intracellular proteins (collagen, elastin, etc.) are impaired, thereby deteriorating the cells.

  It is also known that a decrease in the function of proteasome present in cells affects aging. This proteasome is distributed in both the cytoplasm and the nucleus in eukaryotic cells, and is a huge enzyme complex that degrades proteins inside the cell. For example, the proteasome degrades proteins (cross-linked, glycated or carbonylated proteins) that have been degraded by ultraviolet light or active oxygen, prevents the degraded proteins from accumulating in cells, and contributes to maintaining and improving cell functions. It is known to

  In addition, a protein (heat shock protein: HSP) that repairs a protein or the like in a cell damaged by stress such as ultraviolet light or physical stimulus has attracted attention as a factor for improving cell aging and damage.

  In view of the above points, the present inventors have conducted intensive studies to find a new functional material derived from natural products, and as a result, an extract of peach belonging to Rosaceae has an excellent protein saccharification inhibitory effect, proteasome activity It has an enhancing effect, and an effect of enhancing the expression of heat shock protein, and due to these interactions, a superior cell sounding effect and an anti-aging effect are obtained by blending the extract, for example, an excellent cosmetic We found that it could be provided.

  Conventionally, it is known from Patent Documents 1 to 5 that the extract of peach belonging to the family Rosaceae is used as an anti-aging agent and the like, but the extract of peach is used as a protein saccharification inhibitor, a proteasome activity enhancer, and It has not been known to utilize it as a heat shock protein expression enhancer.

JP 07-309770 A JP-A-10-330222 JP 2001-199867 A JP 2008-247783 A JP 2009-256244 A

The present invention is a protein saccharification inhibitor containing, as an active ingredient, an extract of peach (Prunus persica) belonging to the family Rosaceae.
The present invention is also a proteasome activator comprising, as an active ingredient, an extract of peach (Prunus persica) belonging to the family Rosaceae.
Further, the present invention is a heat shock protein expression enhancer containing, as an active ingredient, an extract of peach (Prunus persica) belonging to the family Rosaceae.
Further, the present invention is a cosmetic containing a protein saccharification inhibitor, a proteasome activator or a heat shock protein expression enhancer containing a peach (Prunus persica) extract as an active ingredient.
In the present specification, the term cosmetic is used in a broad sense including not only so-called cosmetics but also quasi-drugs.

  The present invention is a protein saccharification inhibitor comprising an extract of a peach belonging to Rosaceae as an active ingredient.According to the present invention, it suppresses the accumulation of degraded proteins in cells to prevent senescence and damage of cells, A functional material that can be improved can be provided. The present invention also relates to a proteasome activator comprising an extract of a peach belonging to the family Rosaceae, according to the present invention, which promotes the degradation of degraded proteins by the proteasome and prevents aging and damage of cells. Functional materials that can be improved. The present invention also relates to a heat shock protein expression enhancer containing an extract of a peach belonging to the family Rosaceae as an active ingredient. According to the present invention, it repairs cell damage caused by ultraviolet rays and other external stimulating factors. Functional materials can be provided. Furthermore, since the extract of the present invention further has an active oxygen scavenging effect, it can provide an agent having a remarkably excellent effect of preventing and improving cell aging and damage.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extraction material used in the present invention is peach (Prunus persica) belonging to the family Rosaceae. The seeds of the peach according to the present invention are not particularly limited, and may be any as long as they belong to the peach. For example, white peach, white peach, and golden peach peaches may be mentioned.

  The extract is prepared by first washing the peach site (for example, flowers, leaves, flowers and leaves, or whole plants) with water, if necessary, to remove foreign substances, and then drying as it is or as needed. The mixture is shredded or pulverized as necessary, and is extracted by contacting with an extraction solvent. The extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but it is also possible to use a supercritical extraction method or a steam distillation method.

  Examples of the extraction solvent include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; and ethyl acetate, butyl acetate, and methyl propionate. Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, chloroform and the like, which may be used alone or as a mixture of two or more. Used.

  Among these extraction solvents, in the present invention, water, low-grade, in terms of the effectiveness of the obtained extract, and further from the viewpoint of biosafety and from the viewpoint that it can be widely applied to various products, Hydrophilic solvents such as alcohols or polyhydric alcohols are preferred. Preferred examples of the use of this hydrophilic solvent include, for example, water, lower alcohols (such as ethanol), or polyhydric alcohols (such as 1,3-butylene glycol and glycerin) alone, or water and lower alcohols. And a mixed solvent of water and polyhydric alcohols (1,3-butylene glycol, glycerin, etc.).

  The mixing ratio in the case of using a mixed solvent is, for example, a mixed solvent of water and 1,3-butylene glycol in a volume ratio (the same applies hereinafter) of 1: 3 to 20: 1, and a mixed solvent of water and ethanol. In this case, the ratio is preferably in the range of 1: 3 to 25: 1, and in the case of a mixed solvent of water and glycerin, the ratio is preferably in the range of 1: 1 to 20: 1.

  In addition, the weight ratio of the dry portion of the peach (for example, flowers, leaves, flowers and leaves, or whole plants) to the extraction solvent is preferably in the range of 1: 1 to 1: 150, and more preferably 1: 1. The range is 5 to 1: 120.

  In preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkalinity adjuster such as sodium hydroxide, sodium carbonate and potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid and sulfuric acid is added to the extraction solvent, PH may be adjusted.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, when water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent If so, the extraction temperature is preferably in the range of 0 ° C. to 90 ° C., more preferably in the range of 20 ° C. to 85 ° C., and the extraction time is preferably 0.5 hour to 7 days, more preferably The range is from one hour to three days.

  Prior to or in parallel with the extraction process of the present invention, the extraction site may be subjected to a hydrolysis process if necessary. Thereby, there is a possibility that the extract can be used more effectively by improving the storage stability and the like of the extract.

  When the extract is subjected to an enzymatic hydrolysis treatment, the enzyme includes actinase, papain, a proteolytic enzyme such as chymopapain or pepsin, amylolytic enzyme such as glucoamylase, α-amylase or β-amylase, cellulase, hemicellulase or pectinase. One or two or more selected from the group consisting of fibrinolytic enzymes such as fibrinolytic enzymes and lipases such as lipases may be used. More than one enzyme may be used in combination.

  For example, the amount of the enzyme to be added is preferably in the range of 0.01 to 50% by weight based on the solid content of peach flowers, leaves, flowers and leaves, or the whole plant if it is a whole plant. Preferably it is in the range of 0.1 to 10% by weight.

  The extract prepared as described above is generally adjusted to a pH of 3 to 9, and may be used as it is as a cosmetic compounding agent as it is, or may be used at a desired concentration by concentration under reduced pressure or the like. You may. Further, the extract may be made into a dried product by a conventional method such as a spray drying method.

  In addition, the extract prepared as described above may be refrigerated for a certain period of time and then use the supernatant in order to enhance storage stability and the like.

  When the extract according to the present invention is used, for example, in cosmetics (including quasi-drugs), its dosage form may be emulsion, cream, lotion, essence, pack, lipstick, foundation, liquid foundation, makeup Press powder, blusher, white powder, face wash, body shampoo, hair shampoo, soap and the like. In addition, a hair restorer, a bath agent, and the like can also be used, but of course, the present invention is not limited thereto. In addition, the extract according to the present invention can be incorporated into an oral composition, for example, beverages such as beauty drinks, nutritional drinks, sports drinks, near water, vitamin drinks, mineral drinks, and alcoholic drinks; various soups (Including powdered soups), dairy products, jellies, candy, tablet confections, gums and other foods; tablets, liquid, granular or jelly-like health foods and beverages, etc. It is not limited, and can be blended in foods and drinks that can be taken orally.

  The amount of the extract of the present invention in the cosmetic is generally 0.002 to 1.0% by weight (solid content% by weight, the same applies hereinafter), preferably 0%, as the solid content of the extract in the case of the basic cosmetic. 0.02 to 0.2% by weight, in the case of make-up cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight. The case is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally from 0.00001 to 5.0% by weight, and preferably from 0.0001 to 3.0% by weight. The amount of the extract of the present invention in the oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

  When the extract according to the present invention is incorporated into a cosmetic, in addition to the extract as an essential component, components commonly used in cosmetics, such as oily components, surfactants (synthetic and natural products), and moisturizing An agent, a thickener, a preservative / bactericide, a powder component, an ultraviolet absorber, an antioxidant, a coloring matter, a fragrance and the like can be appropriately compounded as required. In addition, as long as the effectiveness and characteristics of the extract are not impaired, it may be acceptable to mix and combine other physiologically active ingredients.

  Here, as the oily component, for example, olive oil, bergamot oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, Plant-derived fats and oils such as avocado oil, macadamia nut oil, and plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, vaseline, and paraffin wax , Squalane, etc .; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, isopropyl palmitate , Butyrate oleate , 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, and polyoxyethylene alkyl phenyl ether phosphate Surfactants: quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N, Cationic surfactants such as N-dialkylmorphonium salt and polyethylenepolyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of the emulsifier or emulsifier include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (such as sodium), sugar-protein complex, sucrose or its esters, lactose, and soy-based water-soluble. Polysaccharide, complex of soybean-derived protein and polysaccharide, lanolin or a derivative thereof, cholesterol, stevia derivative (enzyme-treated stevia), silicate (aluminum, magnesium, etc.), carbonate (calcium, sodium, etc.), saponin and the like Derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (barley, beans, millet, etc.) can also be blended. In addition, these emulsifiers or emulsifiers may be converted into nanoparticles by high-speed stirring or ultrasonic treatment.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and the like, and saccharides such as trehalose and mucopolysaccharides (eg, hyaluronic acid). Acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Extracts, various amino acids and their derivatives.

  Examples of the thickener include components derived from brown algae, green algae, and red algae such as alginic acid, agar, carrageenan, and fucoidan; silane root (white and white) extracts; pectin, locust bean gum, aloe polysaccharide, and alkaligenes-producing polysaccharide. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of preservatives and bactericides include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophen, chlorhexidine hydrochloride, benzal chloride Plant-derived ethanol such as ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodeinyl urea), propanediol, 1,2-pentanediol, various essential oils, bark dry matter, fermented radish, and sugarcane Or 1,3-butylene glycol.

  As the powder component, for example, sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose And powders of cereals (rice, wheat, corn, millet, etc.), and powders of beans (soybeans, red beans, etc.).

  Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, and 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract and the like .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof (eg, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone and its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate , Adenosine monophosphate). These may be used alone or in combination of two or more.

  Examples of the above kojic acid derivatives include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, examples of the ascorbic acid derivative include sodium L-ascorbic acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium L-ascorbic acid-2-sulfate, and L-ascorbic acid-2. -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleic acid, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as prillyl 2-glyceryl ascorbic acid, acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid -L-ascorbic acid tetrafatty acid esters such as ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivative, ascorbic acid glycerin derivative such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivative of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl The hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and the tranexamic acid derivatives include tranexamic acid esters (eg, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), tranexamic acid amides (for example, tranexamic acid methylamide), and the like. Examples of the resorcinol derivative include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxy. Examples of benzoic acid include nicotinic acid derivatives such as nicotinic acid amide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. .

  Examples of the physiologically active component include placenta extract, soybean extract, saxifrage extract, perilla extract, rice bran extract or hydrolyzate thereof, white poppy extract or hydrolyzate thereof, fermented white poppy, and peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakikisui extract, lotus seed extract or hydrolyzate thereof, lotus seed fermentation product, ginseng extract or hydrolyzate thereof, adlay hydrolyzate, adlay seed fermentation product, Examples include royal jelly fermented product, sake lees extract or ceramide contained therein, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. Can be In addition, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or hydrolyzate thereof, apricot fruit extract, katamenokirinsai Extract of seaweeds such as seagrass, extract of marine flowering plants such as eelgrass, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, fermented rice extract, germinated rice extract or hydrolyzate thereof, germinated rice Fermented product, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot extract, linoleic acid and its derivatives or processed products (eg, liposome-form linoleic acid), collagen derived from animals or fish And its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (such as dipotassium salts), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diiso Ropyramine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, panax ginseng extract or fermented product thereof, red ginseng extract, citrus extract, loofah extract, anaosa extract Peach extract, peach extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, Pau d'Arco bark extract, daylily extract or fermented product, Hibiscus flower extract or fermented product, Hagoromogusa Extract, Cherimoya extract, Mango extract, Mangosteen extract, Funori extract, Oolong tea extract, Beni-fuji extract, Purple orchid extract, Pepper peel or seed coat extract or hydrolyzate, Safflower flower extract, Casablanca Extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, late white citrus extract , Aloe extract, fig flower extract, apple extract, there is bergamot extract or the like.

  Next, the present invention will be described more specifically with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all% mean weight%.

Production Example 1. Preparation of Peach Extract Dried peach flowers of the peach genus Rosaceae were powdered. After adding 1000 g of 50% 1,3-butylene glycol to 10 g of this powder, extraction was performed at 40 ° C. The extract was filtered to obtain 830 g of a brown transparent peach flower extract solution (solid concentration 0.30%).

Production Example 2. Preparation of Peach Extract Dried peach flowers of the peach genus Rosaceae were powdered. After adding 1000 g of purified water to 10 g of this powder, extraction was performed at 40 ° C. The extract was filtered, and 950 g of a brown transparent peach flower extract solution (solid content: 0.36%) was obtained.

Production Example 3 Preparation of Peach Extract Dried peach leaves of the peach genus Rosaceae were powdered. After adding 300 g of 50% 1,3-butylene glycol to 10 g of this powder, extraction was performed at 80 ° C. The extract was filtered to obtain 223 g of a brown transparent peach leaf extract solution (solid content concentration: 0.90%).

Production Example 4. Preparation of Peach Extract Dried peach leaves of the peach genus Rosaceae were powdered. After adding 450 g of purified water to 15 g of this powder, extraction was performed at 80 ° C. This extract was filtered to obtain 358 g (solid content concentration: 1.03%) of a brown transparent peach leaf extract solution.

Formulation Example 1. Lotion [A component] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butyl paraben 0.1
[B component]
Extract solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Appropriate amount of potassium hydroxide Purified water Amount that total amount becomes 100 parts [C component]
Appropriate amount of fragrance After heating the A component and the B component to 80 ° C. or more, the B component was added to the A component, the mixture was stirred, and homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling to 50 ° C., the C component was added and mixed with stirring, and further cooled to 30 ° C. or lower to obtain a lotion.

Formulation example 2. Lotion Toner was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract of Preparation Example 2 was used instead of the extract of Preparation Example 1 contained in the B component of Formulation Example 1.

Formulation example 3. Lotion Toner was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Preparation Example 3 was used instead of the extract of Preparation Example 1 contained in the B component of Formulation Example 1.

Formulation Example 4. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Preparation Example 4 was used instead of the extract of Preparation Example 1 contained in the B component of Formulation Example 1.

Formulation Example 5. Emulsion [A component] part liquid paraffin 6.0
Hexaran 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 1.5
Extract of Production Example 3 1.5
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
The amount of purified water to be 100 parts [component C]
Appropriate amount of fragrance The above components A and B were each heated to 80 ° C. or higher, and then mixed by stirring. After cooling to 50 ° C., the C component was added and further stirred and mixed to obtain an emulsion.

Formulation Example 6. Emulsion The emulsion was prepared in the same manner as in Formulation Example 5 except that 2.0 parts of tranexamic acid was used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Formulation Example 5. I got

Formulation Example 7. Emulsion The emulsion was prepared in the same manner as in Formulation Example 5 except that 3.0 parts of arbutin was used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Formulation Example 5. Obtained.

Formulation Example 8. Emulsion In the same manner as in Formulation Example 5, except that 3.0 parts of nicotinamide was used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Formulation Example 5, An emulsion was obtained.

Formulation Example 9. Emulsion In the same manner as in Formulation Example 5, except that 5.0 parts of Sophora cinnamon extract were used in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the B component of Formulation Example 5, An emulsion was obtained.

Formulation Example 10. Emulsion [A component] part liquid paraffin 6.0
Hexaran 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 2 2.0
Extract of Production Example 4 2.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water The amount that makes the total amount 100 parts

Formulation Example 11. Lotion [ingredient] part Extract of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methyl paraben 0.2
Dipotassium glycyrrhizinate 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Formulation Example 12. Lotion A lotion was obtained in the same manner as in Preparation Example 10, except that 10.0 parts of the extract of Preparation Example 4 was used instead of the extract of Preparation Example 2 among the components of Preparation Example 11.

Formulation Example 13. Essence [ingredient] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methyl paraben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid 0.1
Extract of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water An amount that makes the total amount 100 parts. After dissolving hyaluronic acid in purified water, the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Embodiment 14 FIG. Liquid foundation [A component] part stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propyl paraben 0.05
[B component]
Extract of Production Example 1 5.0
Sodium carboxymethyl cellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methyl paraben 0.1
The amount of purified water to be 100 parts [component C]
Titanium oxide 8.0
Talc 4.0
Appropriate amount of coloring pigment The above components A and B were each heated and mixed and stirred. The mixture was heated again, the above-mentioned component C was added, the mixture was poured into a mold, and the mixture was stirred until it reached room temperature to obtain a liquid foundation.

Formulation example 15. Body shampoo [A component] part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methyl paraben 0.1
[B component]
Extract of Production Example 2 5.0
1,3-butylene glycol 2.0
Amount of purified water to total 100 parts A component and B component are each heated to 80 ° C and uniformly dissolved, then B component is added to A component, stirring is continued and cooled to room temperature to obtain body shampoo Was.

Formulation example 16. Cosmetic for hair growth [Ingredients] part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-menthol 0.8
Tamasakitsu Lafuji Root Extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract of Production Example 1 2.0
Extract of Production Example 2 2.0
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine qs ethanol 20
The amount of purified water to be 100 parts was sufficiently stirred and mixed with the above components to obtain a hair restorer.

Formulation example 17. Hair shampoo [A component] part N-coconut oil fatty acid methyltaurine sodium 10.0
Sodium polyoxyethylene (3) alkyl ether sulfate 20.0
Betaine lauryl dimethylaminoacetate 10.0
Coconut oil fatty acid diethanolamide 4.0
Methyl paraben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Amount of purified water to become 100 parts A component and B component are each heated to 80 ° C. and uniformly dissolved. Then, B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo. Was.

Embodiment 18 FIG. Hair conditioner [A component] part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyl dimethyl ammonium chloride 1.5
Stearyl trimethyl ammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methyl paraben 0.1
[Component B] Part Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Amount of purified water so that the total amount becomes 100 parts A component and B component were each heated to 80 ° C. and uniformly dissolved, and then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Test Example 1 Evaluation test of protein glycation inhibitory effect In this test, the AGE generation inhibitory effect, that is, protein glycation inhibitory effect, was evaluated using the fluorescence intensity and absorbance of BSA (bovine serum albumin) generated by reacting with glucose as indexes. Next, 50 μL of the prepared solution, 50 μL of a 40 mg / mL BSA aqueous solution, 50 μL of a 2.0 M glucose aqueous solution, and 50 μL of a PBS (−) solution containing 0.2% paraben and 5.0% 1,3-butylene glycol Was mixed and stirred to prepare a sample solution. Sample solutions were prepared so that the final concentrations of the extract solution of Production Example 1 were 1.0% and 2.0%, respectively. Next, the sample solution was allowed to stand at 50 ° C. for 7 days, and after 7 days, fluorescence was generated for each sample solution (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)). And absorbance (wavelength: 405 nm: microplate reader (Model 680, manufactured by Bio-Rad)) were measured. In addition, the same operation was performed for the case where no sample was added (Control) using 50% 1,3-butylene glycol in place of the extract of Production Example 1, and the obtained fluorescence measurement value and absorbance measurement value were obtained. The relative value (%) between the fluorescence measurement value and the absorbance measurement value of each sample solution was determined and defined as the protein saccharification rate (%).

Table 1 shows the results of Test Example 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract according to Production Example 1 of the present invention had a remarkably excellent effect of inhibiting protein saccharification in a concentration-dependent manner.

Test example 2. Evaluation test of fibroblast proteasome activity enhancing effect Human dermis-derived fibroblasts NB1RGB were seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing Eagle's minimal essential medium containing 0.5% NCS, at 37 ° C. After pre-incubation for 1 day under the condition of 5.0% CO ₂ , the extract (sample solution) of Production Example 1 was added to the medium to a concentration of 0.5% and 1.0% (as a solution). Then, the cells were further cultured under the same conditions for 3 days. Thereafter, the cell culture supernatant in each test section was removed, and 100 μL of a cell lysis solution (Tris-HCl buffer (pH 8.0) containing 1% Tween 20: 12 mM Trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid) was added. The resulting cells were crushed to obtain a crude enzyme solution. 50 μL of the crude enzyme solution was separated on another plate, and 10 μL of a proteasome activity inhibitor (cell lysate containing 2 mM epigallocatechin gallate) was added thereto, and this was used for non-proteasome activity measurement. To the remaining crude enzyme solution, 10 μL of a cell lysate was added to adjust the volume, and this was used for measuring the total substrate resolution. To each plate, 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added, and reacted at 37 ° C. for 1 hour. After the completion of the reaction, the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as proteasome activity. The same operation as above was performed for the case where no sample was added (Control) in which 50% 1,3-butylene glycol was added instead of the sample solution of Production Example 1, and each sample was added to the obtained proteasome activity. Was determined as the proteasome activity rate (%).

[Table 2]

  As shown in Table 2, it was confirmed that the extract of Production Example 1 according to the present invention enhanced proteasome activity of fibroblasts in a concentration-dependent manner.

Test Example 3 Evaluation Test of Epidermal Cell Proteasome Activity Enhancement Effect Normal human skin-derived epidermal cells (NHEK) were seeded at 5 × 10 ³ cells / well in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo) at 37 ° C., 5. After pre-culturing for 1 day under the condition of 0% CO ₂ , the sample solution of Production Example 1 was added to the medium to a concentration of 0.5% and 1.0% (as a solution), and further under the same conditions. Cultured for 3 days. Thereafter, the cell culture supernatant in each test section was removed, and 100 μL of a cell lysis solution (Tris-HCl buffer (pH 8.0) containing 1% Tween 20: 12 mM Trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid) was added. The resulting cells were crushed to obtain a crude enzyme solution. 50 μL of the crude enzyme solution was separated on a separate plate, and 10 μL of a proteasome activity inhibitor (a cell lysate containing 2 mM epigallocatechin gallate) was added thereto, and this was used for non-proteasome activity measurement. To the remaining crude enzyme solution, 10 μL of a cell lysate was added to adjust the volume, and this was used for measuring the total substrate resolution. To each plate, 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added, and reacted at 37 ° C. for 1 hour. After the completion of the reaction, the fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as proteasome activity. The same operation as above was performed for the case where no sample was added (Control) in which 50% 1,3-butylene glycol was added instead of the sample solution of Production Example 1, and each sample was added to the obtained proteasome activity. Was determined as the proteasome activity rate (%).

[Table 3]

  As shown in Table 3, it was confirmed that the extract of Production Example 1 according to the present invention enhanced proteasome activity of epidermal cells in a concentration-dependent manner.

Test Example 4. Evaluation test of heat shock protein (HSP) expression enhancement effect Human dermal-derived fibroblasts NB1RGB were adjusted to 6 × 10 ⁵ cells / mL in Eagle's minimum essential medium containing 0.5% NCS, and 1 mL was inoculated in a φ6 cm petri dish. Then, the cells were cultured at 37 ° C. under 5% CO ₂ and saturated steam. After culturing for 24 hours, a culture solution containing the extract of Production Example 3 (to which the extract of Production Example 3 was added so that the final concentration was 1.0% as a solution with respect to the total amount of the culture solution) was further added. The cells were added and cultured. As a comparative control, a culture solution containing only 50% 1,3-butylene glycol instead of the extract of Production Example 2 (the final concentration of 50% 1,3-butylene glycol relative to the total volume of the culture solution was reduced to 1%). (Control group) to which a test sample (control) was added. After culturing for 24 hours, the cells in each test section were collected with 1 mL of Trizol reagent (Invitrogen). To the collected cells, 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added, mixed with stirring, and centrifuged at 15,000 rpm for 15 minutes at 4 ° C. in a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the collected aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, mixed by stirring, and centrifuged at 15,000 rpm at 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, stirred, washed, and centrifuged at 15,000 rpm at 4 ° C. for 15 minutes to collect a precipitate. The collected total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) (manufactured by Takara Bio Inc.) The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is expressed in a certain amount in common in many tissues and cells, and is always expressed and is essential for the maintenance and growth of cells. Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, when the expression level of the G3PDH gene was fixed, the expression level of each gene in each test plot was compared. In this test system, the relative value of the expression level of each gene in the other test plots was determined, assuming that the expression level of each gene in the control plot was 100.

[Table 4]

[Table 5]

As shown in Tables 4 and 5, it was confirmed that the extract of Production Example 3 according to the present invention enhanced the gene expression of HSP32 and HSP72.

Test Example 5. Evaluation test of DPPH radical scavenging effect First, 2.4 parts of DPPH (1,1-diphenyl-2-picrylhydrazyl) were dissolved in 20 parts of ethanol, and 20 parts of purified water was added thereto to prepare a DPPH solution. . To 24 parts of this DPPH solution, 19.2 parts of an 18 v / v% ethanol solution and 4.8 parts of a 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH-added solution. Further, in order to subtract the influence of the color tone of the extract itself on the test, a solution obtained by mixing an 18 v / v% ethanol solution and a 2 M acetic acid-sodium acetate buffer solution using a 50 v / v% ethanol solution instead of the DPPH solution was used. A control solution was used. Next, the extract solution of Production Example 1 prepared as described above was diluted with purified water to prepare a sample solution. Here, the sample solution was prepared so that the final concentration (concentration as a solution) of the extract solution of Production Example 1 was 0.5%, 1.0%, and 2.0%, respectively, with respect to the total amount. Seed concentrations were used. This sample solution was mixed with the DPPH-added solution or the control solution at a ratio of 1: 3, allowed to stand at room temperature for 10 minutes, and then, when each test solution was mixed with the DPPH-added solution, the absorbance at 550 nm was measured. Was mixed with a control solution, and the difference from the absorbance at 550 nm was measured to confirm the remaining amount of DPPH radical. At the same time, a 50% 1,3-butylene glycol aqueous solution was used in place of the extract solution of Production Example 1 as a control, and the same operation as above was performed. The relative value of the DPPH radical remaining rate was determined. Further, in order to confirm whether the test system is functioning normally, the same test was conducted when water-soluble vitamin E (final concentration: 10 μM) was added as a positive control instead of the sample solution.

Table 6 shows the results of Test Example 5.
[Table 6]

  As shown in Table 6, the extract according to Production Example 1 of the present invention was shown to have a significantly superior DPPH radical scavenging effect in a concentration-dependent manner.

Claims (2)

A mixed solvent of flowers of water and 1,3-butylene glycol peach belonging to Rosaceae (Rosaceae) (Prunus persica) (mixing ratio of water and 1,3-butylene glycol 1: 3 to 20: 1) extract A protein saccharification inhibitor containing as an active ingredient. A mixed solvent of flowers of water and 1,3-butylene glycol peach belonging to Rosaceae (Rosaceae) (Prunus persica) (mixing ratio of water and 1,3-butylene glycol 1: 3 to 20: 1) extract A proteasome activator containing as an active ingredient.