JP5054945B2 - Cosmetics - Google Patents

Description

  The present invention relates to a cosmetic comprising a novel plant fermented product as an active ingredient. Specifically, it suppresses the production and release of chemical mediators, and further applies to skin by ultraviolet rays, nitrogen oxides and sulfur oxides in the atmosphere. Effective in preventing and improving skin inflammation, allergic symptoms, itching, pain, etc. by preventing the extracellular matrix from being fragmented by active oxygen such as radicals generated in the skin due to irritation The present invention relates to a cosmetic that comprises the novel fermented plant shown and is useful for maintaining or improving the skin in a healthy and youthful state.

The environment that surrounds us is surrounded by substances that can cause inflammation and allergies, such as house dust, exhaust gas, and mites. Nitrogen oxides and sulfur oxides contained in ultraviolet rays and exhaust gases cause oxidative damage to the skin and alter biological components, thereby generating antigens in the skin. For the onset of inflammation and allergies caused by these antigens, the cells are stimulated by crosslinking the Fab chain of IgE antibody of basophils and mast cells, and histamine, serotonin, eosinophil migration factor, etc. are released. It is known that degranulation is involved. Furthermore, basophils and mast cells stimulated by antigens activate phospholipase A2 by the influx of calcium ions to form an arachidonic acid cascade, and prostaglandins, which are inflammatory chemical mediators, are secreted to cause inflammation. It is known to trigger.
Anti-inflammatory agents such as glycyrrhizic acid and allantoin have been proposed for the purpose of preventing inflammation caused by ultraviolet rays and improving symptoms, and skin external preparations containing these have been put on the market.
However, the above-mentioned anti-inflammatory agents must be formulated at a considerably high concentration in order to obtain a sufficient effect. For this reason, problems may arise in terms of safety and formulation stability, and there are currently no satisfactory ones in all aspects of safety, formulation stability and effects.

  In view of the problems of the prior art as described above, the present inventors oxidize biological components in the skin by ultraviolet rays or the like to become antigens, or external stimulating factors surrounding the environment enter the skin and become antigens. It has excellent preventive or symptom-improving effects on skin inflammation and itching caused by inducing degranulation, and it contains cosmetic ingredients with high biological safety, as well as these ingredients. As a result of intensive research and examination to provide a cosmetic that protects the skin from damage caused by inflammatory reactions based on ultraviolet rays and environmental stimuli, and maintains or improves the state in a healthy and healthy state. It came to complete.

That is, the present invention is a cosmetic comprising as an active ingredient a fermented product obtained by fermenting a plant of the genus Hemerocallis and / or an extract thereof with yeast.
In the present invention, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

The forget-me-not plant and / or yeast extract of the extract thereof used as an active ingredient in the cosmetic composition of the present invention has an action of strongly suppressing degranulation of basophils and mast cells induced by antigens, and is also inflammatory It also has the action of suppressing the production of prostaglandin E2, which is a chemical mediator of the skin, and the synergistic effect of these two actions prevents skin inflammation and itching caused by antigenic substances in the external environment or in vivo. Remarkably suppresses and has an effect of protecting the skin from damage such as rough skin caused by inflammation.
In addition, the yeast fermented product of the present invention also has radical scavenging ability. Therefore, according to the yeast fermented product of the present invention, biological components inside the skin are oxidized by ultraviolet rays or nitrogen oxides in the atmosphere. Thus, it is possible to suppress the mechanism of generating an antigen, and a more effective anti-inflammatory effect can be achieved. In addition, since it has radical scavenging ability, it can also have the effect of preventing cell damage due to in vivo radicals and fragmentation of extracellular matrix components such as hyaluronic acid.
Therefore, according to the cosmetic composition of the present invention containing such a fermented yeast product, skin inflammation damage and further oxidative damage due to ultraviolet rays, irritants in the external environment, and contaminants are further prevented or improved. It is kept healthy and youthful.

  Regarding the use of lily family forget-me-not plants as cosmetics or cosmetic ingredients, JP-A-2006-143670 discloses that an extract of the plant has an anti-aging action and a whitening action, and has a comprehensive cosmetic effect on the skin. An effect is disclosed.

JP 2006-143670 A

  However, in the case of the above-mentioned conventional technique, the components contained in the forget-me-not plant are extracted as they are and used as cosmetic ingredients, and the forget-me-not plant and / or the extract thereof are used for yeast fermentation. The technical idea is clearly different from the present invention in which the fermented product obtained here is used as a source and used as a cosmetic blending raw material. Therefore, of course, in the above prior art, the fermented product of the present invention prevents the degranulation of basophils and mast cells, and suppresses the release of chemical mediators such as histamine that is a flame retardant, Based on its radical scavenging ability, it has the function of inhibiting the production of antigens due to the oxidation of biological components, and also the action of inhibiting the production of prostaglandin E2, which is a causative agent of inflammation due to UV exposure. In addition, the cosmetic composition of the present invention, which exhibits an excellent anti-inflammatory effect based on a new mechanism of action unprecedented by comprehensive contribution and the yeast fermented product as an active ingredient, makes the skin an external environment. No disclosure has been made regarding the effect of protecting against inflammation damage and oxidative damage caused by factors, and these facts are not clear until the present invention is achieved. It is where became one.

The fermented yeast of Hemerocallis plant and / or its extract used as an active ingredient in the cosmetics of the present invention includes house dust, exhaust gas, mites, etc. surrounding the environment, and oxidized and denatured biological components, Prostaglandins that suppress the release of chemical mediators such as histamine, serotonin, and eosinophil migration factor from basophils and mast cells by substances that cause inflammation and allergies, and are inflammatory chemical mediators By suppressing the production of E2, it exerts the effect of suppressing inflammation and itching occurring in the skin. Furthermore, when this is used to protect against skin inflammatory damage caused by ultraviolet rays, unlike conventional anti-inflammatory agents, it suppresses the release of chemical mediators that cause inflammation and further suppresses the oxidation of biological components. In addition, by suppressing the production of prostaglandin E2 production, production of inflammatory chemical mediators itself is also suppressed, and excellent anti-inflammatory effects are exhibited. In addition, since the yeast fermented product of the present invention is composed of plant-derived components, it has little skin irritation and the like, and is extremely excellent in biological safety.
The cosmetic composition of the present invention comprising such a yeast fermented product as an active ingredient can protect the skin from inflammatory reaction damage and oxidative damage when applied to the skin, and keep the skin in a healthy and youthful state. There is an effect to improve. In addition, the cosmetic of the present invention is highly safe, and there is no fear of adversely affecting the skin even after long-term use.

Hereinafter, the present invention will be described in detail.
Examples of the plant belonging to the genus Forget-me-not used in the present invention include Hemerocallis fulva, North Yellow Flower Vegetables (Hemerocallis lilio-asphodelus), Small Yellow Flower Vegetables (Hemerocallis mirror), and Leafy Leaves (Hemerocallis). In addition, gold cypress, which is a crude drug based on moths of forget-me-not plants such as camellia, can be used.
Among these forget-me-not plants, from the viewpoint of the degranulation inhibitory action and the prostaglandin E2 production inhibitory action of yeast fermented products, it is possible to use a gold medicinal vegetable or a herb (Hemerocallis flva) that is a herbal medicine based on a moth of the forget-me-not plant. Use is most preferred.

  When these lily family Forget-me-not plants and / or extracts thereof are fermented with yeast, the fermentation site of the plant is not particularly limited, and whole plants, leaves, flowers, stamens, pistils, stems, rhizomes, seeds (grains) ), Etc. can be used, but from the viewpoint of the effectiveness of the yeast fermented product obtained, the use of cocoons is preferable, and the use of gold needles, which are herbal medicines based on moths of forget-me-not plants, is the most important. preferable.

Fermentation of forget-me-not plants by yeast can be performed, for example, as follows.
First, as an assimilation source for fermentation, a forget-me-not plant itself (hereinafter sometimes referred to as a plant) may be used, or an extract obtained by extracting a plant with an appropriate medium may be used. . Moreover, when using an extract, it does not interfere at all even if it uses for a fermentation with a plant body included, without removing the plant body of a to-be-extracted object by solid-liquid separation.
Among these methods, the fermented product is fermented from the viewpoint of the degranulation inhibitory action and prostaglandin E2 production inhibitory action of the obtained fermented product, as well as the stability and smell when blended in cosmetics. Most preferred.
Forget-me-not plants that are subjected to fermentation or used to prepare an extract may be raw or may be dried or semi-dried in advance. Moreover, although what was extract | collected can be used as it is as a shape, fermentation efficiency or extraction efficiency can be raised if it shreds or grind | pulverizes and refines | miniaturizes.

When fermentation is performed using a plant body as an assimilation source, the plant body is first suspended in a solvent. Solvents for suspending plants include water or water and lower alcohols (methanol, ethanol, isopropanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,2-pentanediol) , Glycerin and the like) and sugars such as glucose, fructose and sucrose may be added to these solvents. Among these solvents, water is used alone from the viewpoint that yeast is most likely to exert its action and also avoids the production of fermentation by-products based on the presence of assimilation components other than those of Forget-me-not plants. Is most preferred.
The mixing ratio of the plant body and the solvent is generally in the range of 1: 1 to 1: 100, preferably 1:10 to 1:50 in terms of the dry weight of the plant body.

  When using an extract of a plant body as a fermentation assimilation source, as an extract preparation method, a part to be extracted, for example, koji, is pre-washed and dried as necessary, preferably further chopped or pulverized and then immersed For example, a method of contacting with an extraction solvent by an appropriate means such as a countercurrent extraction method or a countercurrent extraction method can be employed. A supercritical extraction method may also be used.

As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate, glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl but ethers such as ether; n-hexane, toluene And hydrocarbon solvents such as chloroform, and the like. These may be used alone or in combination of two or more.
Among these extraction solvents, water, lower alcohols and polyhydric alcohols from the viewpoint of the anti-inflammatory action and radical scavenging activity of the yeast fermentation product of the obtained extract, and also the point of easy transition to the fermentation process It is preferable to use one kind of single solvent selected from the above or a mixed solvent of two or more kinds, and it is most preferable to use water alone.

The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of a mixed solvent of 1 to 15: 1 or water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 15: 1.
When a solvent other than water or a mixed solvent of water and a solvent other than water is used as the extraction solvent, the solvent other than water is once distilled off by an operation such as concentration before moving to the fermentation step, and then solid. It is redissolved in water so as to be about 0.01 to 10% as a fraction, or diluted with water to be an assimilation source for fermentation.

  These plant suspensions or extract solutions are sterilized before they are subjected to fermentation with yeast to remove germs that hinder yeast fermentation. As a method for removing this germ, it is possible to use a method in which the plant is washed with sterilizing ethanol in advance and then suspended in a sterile solvent such as sterile water or extracted with a sterile solvent. After turbidity or extraction with a solvent, the suspension or extract solution may be sterilized by heat sterilization or the like.

Next, this suspension or extract solution is put into a fermentation tank, and 10 ⁷ to 10 ⁸ yeasts / mL of yeast are inoculated therein to cause fermentation.
Examples of yeasts used for fermentation include Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergen, Saccharomyces carlsbergen, Saccharomyces carlsbergen Yeasts of the genus Saccharomyces; Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei and other yeasts of the genus Torlasaspora rosei (Zygosa)
ccharomyces rouxii), Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces saccharis Candida versatilis), Candida etchellsii, Candida kefyr, Candida sake, Candida scottii, and other yeasts from the genus Candida scottii. Among them, Saccharomyces cerevisiae is most preferable because it is most widely used for foods and has a strong fermenting power.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably in the range of 35 to 40 ° C., which is the optimum temperature for yeast growth. The fermentation period is generally in the range of 3 to 30 days, preferably 3 to 20 days at the optimum temperature, but the enzyme hydrolysis treatment described later is performed on the plant body or its extract at the same time or prior to the fermentation. In general, the fermentation time can be shortened to a range of 3 hours to 10 days, preferably 10 hours to 3 days. If the fermentation period is shorter than the above general range, fermentation tends to be inadequate and the effectiveness of the fermented product tends to decrease.On the other hand, even if it exceeds the upper limit, there is no further increase in effectiveness. Neither is it preferable that coloring or an increase in fermentation odor occurs.

In carrying out the above yeast fermentation, in order to more effectively use the components of the Forget-me-not plant as an assimilation source of yeast, an enzyme is added to the suspension or extract solution before or simultaneously with the yeast inoculation. In addition, it is preferable to subject the Forget-me-not plant or its extract to an enzymatic hydrolysis treatment. Such an enzyme treatment is useful for fermentation, for example, when the Forget-me-not plant root or its extract is used as a fermented product. This is particularly effective when there are few components that can remain as they are in the chemical source component.
In this case, as the enzyme, at least one selected from proteolytic enzymes such as actinase and papain, amylolytic enzymes such as glucoamylase and α-amylase, and fibrinolytic enzymes such as cellulase and hemicellulase are used. Good results can be obtained by using a combination of at least one enzyme each selected from these three enzyme groups.
The total amount of the enzyme added is preferably 0.01 to 10% by weight, more preferably 0.1 to 1.0% by weight, based on the solid content of the forget-me-not plant in the suspension. Or 0.001 to 5 weight% is preferable in total with respect to solid content of the extract of a forget-me-not plant, and 0.01 to 0.5 weight% is more preferable.
As treatment conditions such as temperature and time, if the enzyme treatment is performed before yeast fermentation, the treatment should be performed for 1 to 24 hours near the optimum temperature of each enzyme, while it may be performed simultaneously with yeast fermentation. For example, the same conditions as in the yeast fermentation are applied.

  When the above yeast fermentation is finished, for sterilization of the yeast, or when combined with enzyme treatment, the sterilization of the yeast and the inactivation of the enzyme are combined. After the heat sterilization treatment to the extent, the liquid phase is separated as it is or generally and preferably by solid-liquid separation means such as filtration or centrifugation, and if necessary, the pH is adjusted to the pH range of ordinary cosmetics. The pH is adjusted to 6 to 8, and if necessary, it is adjusted to an appropriate concentration by dilution or concentration and used as a cosmetic raw material. In some cases, the liquid phase after solid-liquid separation may be blended into a cosmetic after being powdered according to a conventional method such as spray drying or freeze drying.

  Cosmetics comprising the forget-me-not plant and / or yeast extract of the extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, lipsticks, foundations, liquids, etc. Makeup cosmetics such as foundations, makeup press powders, hair shampoos, hair rinses, hair treatments, hair conditioners such as conditioners, hair dyes, hair conditioners, cleansing cosmetics such as facial cleansers, body shampoos, soaps, etc. Examples of such a bath agent include, but are not limited to these.

  The amount of yeast fermented product of Forget-me-not plant and / or extract thereof in the cosmetics of the present invention is generally 0.001 to 8% by weight, preferably, for basic cosmetics, for example, as a solid content of the fermented product. In the range of 0.01 to 5% by weight, for makeup cosmetics, generally 0.001 to 5% by weight, preferably in the range of 0.01 to 2% by weight, and for hair cosmetics, generally 0.001 to 10% by weight. %, Preferably in the range of 0.01 to 5% by weight, and for cleansing cosmetics, it is generally in the range of 0.001 to 10% by weight, preferably 0.01 to 5% by weight.

In addition to the essential fermented forget-me-not plant and / or yeast extract of the extract thereof, the cosmetics of the present invention include components usually used in cosmetics, such as oily components, surfactants, moisturizers, thickening agents. An agent, antiseptic / bactericidal agent, powder component, ultraviolet absorber, physiologically active component (whitening component, anti-aging / skin-cleaning / wrinkle-preventing component, etc.), pigment, fragrance and the like can be appropriately blended as necessary.
Moreover, the anti-inflammatory agent or antioxidant normally used can also be mix | blended unless the effectiveness and the characteristic of the yeast fermented product of the forget-me-not genus plant of this invention and / or its extract are impaired.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), zyzyphus juazeiro: Rhamnaceae ) An extract or the like can also be blended.

  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, and mucopolysaccharides. (For example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, seafood Examples include derived collagen and derivatives thereof, various amino acids and derivatives thereof, and sphingolipids.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, beech extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, etc. Is mentioned.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazoline urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. Silk powder, cellulosic powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, sucha akuhi extract, yukinoshita extract, rice bran Extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (barley, beans, millet), white coconut hydrolyzed extract, murasakixikib extract, pandanus amari riforius Amaryllifolius Roxb.) extract, Arcangelicia flava Merrilli extract, chamomile extract (trade name: Chamomile ET), kombu (e.g. Mitsuishi Kombu), seaweed extract such as Anaaasa, Extracts of seaweed such as tuna, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid), 2,5-dihydroxybenzoic acid derivatives, etc. are also used as ingredients for preventing skin aging, beautifying and wrinkling. Or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin , Α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract and other herbal extract, rice extract hydrolysate, Rice bran extract hydrolyzate, rice bran Examples include fermented extract, Sohakuhi extract, Zyzyhus juazeiro extract, and sphingolipid.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of the vitamin E derivative include vitamin E nicotinate and vitamin E linoleate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

  Anti-inflammatory agents include glycyrrhizic acid and allantoin.

  Furthermore, examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, white coconut extract, rice extract and the like.

  Next, although a manufacture example, a test example, and an Example (formulation example of cosmetics) are given and this invention is demonstrated further more concretely, this invention is not limited to them. In the following, all parts are by weight, and all% are by weight.

Production Example 1
Purified water 1500 g, cellulase 1.5 g and pectinase 1.5 g are mixed with 150 g of gold needle vegetables, extracted for 3 hours at 40 ° C. and subjected to enzymatic degradation, then heated at 80 ° C. for 1 hour to lose the enzyme. I made it live. Furthermore, the extract suspension obtained here was sterilized by heating. The extract suspension yeast (Saccharomyces cerevisiae) were inoculated 10 ⁸ cells / mL in and allowed to stand for 3 days incubation at 30 ° C. under a stream of nitrogen. After culturing, the mixture was sterilized by heating, and the culture broth was filtered to perform deodorization and decoloration treatment, thereby obtaining 1300 g (solid content concentration: 1.32%) of a fermented yeast fermented vegetable solution.

Production Example 2
In the same manner as in Production Example 1 except that Saccharomyces cerevisiae was used in place of Saccharomyces cerevisiae, 1280 g (solid content concentration 1.59%) of a fermented yeast vegetable yeast solution was obtained.

Production Example 3
In the same manner as in Production Example 1, except that Saccharomyces cerevisiae was used instead of Saccharomyces cerevisiae, 1350 g (solid content concentration: 1.12%) of a fermented yeast vegetable yeast solution was obtained.

Production Example 4
In the same manner as in Production Example 1, except that Saccharomyces cerevisiae was used instead of Saccharomyces cerevisiae, 1010 g of a fermented yeast fermented yeast solution (solids concentration 2.01%) was obtained.

Production Example 5
In the same manner as in Production Example 1, except that Saccharomyces cerevisiae was used in place of Saccharomyces cerevisiae, 920 g (solid content concentration 1.75%) of a fermented yeast vegetable yeast solution was obtained.

Production Example 6
1400 g (solid content concentration: 2.95%) of a fermented yeast solution of gold needle vegetables was obtained in the same manner as in Production Example 1 except that the culture period was 18 hours.

Production Example 7
880 g of yeast fermented yeast solution (1.42% solid content) was obtained in the same manner as in Production Example 1 except that an extract suspension solution was prepared using a cut of licorice stalk instead of gold needle vegetables. It was.

Production Example 8
1010 g of yeast fermented solution of whole plant of licorice (solids concentration 2.31%) except that the extract suspension solution is prepared by using shredded whole plant of licorice instead of gold needle vegetables Got.

Production Example 9
Purified water (900 g) was mixed with 100 g of finely chopped gold needle vegetables and extracted at 80 ° C. for 2 hours, followed by filtration to obtain about 500 g of a light brown transparent extract solution (solid content concentration 5.77%). The extract solution obtained here was sterilized by heating. After adding 0.2 g of papain, 0.2 g of cellulase and 0.2 g of pectinase to this extract solution, inoculated with 10 ⁸ yeast / mL yeast (Saccharomyces cerevisiae) and left at 37 ° C. for 18 hours under a nitrogen stream. Cultured. After completion of the culture, the mixture was sterilized by heating, and the culture broth was filtered to perform deodorization and decoloration treatment to obtain 360 g (solid content concentration: 2.79%) of a fermented yeast vegetable yeast solution.

Production Examples 10-13.
Other than using Saccharomyces cerevisiae instead of Saccharomyces cerevisiae in the same manner as Production Example 9, 330 g (solid content of 3.05%) of the gold needle vegetable yeast fermented product solution (Production Example 10) and Saccharomyces chevaleri In the same manner as in Production Example 9, 410 g of the gold needle vegetable yeast fermented solution (solid content 2.21%) (Production Example 11) and 330 g of the gold needle vegetable yeast fermented solution in the same manner as in Production Example 9 except that Digosaccharomyces lactis was used. (Production Example 12), except that Candida Versatilis was used in the same manner as in Production Example 9, and 360 g of a fermented yeast fermented yeast solution (solid content 1.97%) (Production Example 13), Each got.

Production Example 14
Purified water (900 g) was mixed with 100 g of finely chopped gold needle vegetables and extracted at 80 ° C. for 2 hours, followed by filtration to obtain about 500 g of a light brown transparent extract solution (solid content concentration 5.77%). The extract solution obtained here was sterilized by heating. The extract solution yeast (Saccharomyces cerevisiae) were inoculated 10 ⁸ cells / mL and cultured standing 14 days at 37 ° C. under a stream of nitrogen. After completion of the culture, the mixture was sterilized by heating, and the culture broth was filtered to perform deodorization and decoloration treatment to obtain 360 g (solid content concentration 4.80%) of the fermented yeast solution of gold needle vegetables.

Production Example 15.
300 g of the gold needle vegetable yeast fermented solution obtained in the same manner as in Production Example 1 was freeze-dried and pulverized to obtain 4 g of fermented yeast powder of gold needle vegetables.

Example 1. Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate [component B]
Fermented product solution of Production Example 1 10.0
Glycerin 5.0
Methylparaben 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts
(Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the fermented product solution of Production Example 9 was used instead of the fermented product solution of Production Example 1 in the component B of Example 1.

Example 3. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Fermented product solution of Production Example 1 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 4 Lotion [component] part Fermented product solution of Production Example 9 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 5 FIG. Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl ether 0.5
Butylparaben 0.1
[B component]
Fermented product solution of Production Example 2 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glyco 5.0
Methylparaben 0.1
Potassium hydroxide Appropriate amount Purified water Amount of 100 parts [C component]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 6 Emulsion In the component B of Example 3, an emulsion was obtained in the same manner as in Example 3 except that the fermented product solution of Production Example 3 was used instead of the fermented product solution of Production Example 1.

Example 7. Emulsion In the component B of Example 3, an emulsion was obtained in the same manner as in Example 3 except that the fermented product solution of Production Example 4 was used instead of the fermented product solution of Production Example 1.

Example 8. Emulsion An emulsion was obtained in the same manner as in Example 3 except that the fermented product solution of Production Example 5 was used instead of the fermented product solution of Production Example 1 in the component B of Example 3.

Example 9 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Fermented product solution of Production Example 1 10.0
L-ascorbic acid-2-glucoside 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total [Component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 10 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 11 Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate and 2.0 parts of sodium L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 12 Emulsion In the component B of Example 9, the emulsion was prepared in the same manner as in Example 9, except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 13 Latex In the component B of Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of * Snow * HP ", solid content concentration 3.5%) were used.

Example 14 Latex In the component B of Example 9, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, white seed (Brassica Alba) seed extract (trade name, manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of “Sinablanca-WH” (solid content concentration 1.0%) were used.

Example 15. Emulsion Example 9 except for using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 9 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used. In the same manner as in No. 9, an emulsion was obtained.

Example 16 Latex In the component B of Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice extract hydrolyzate (trade name “Oryzenoble, manufactured by Technoble Co., Ltd.) A solid emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of a solid content concentration of 1.5% was used.

Example 17. Lotion [component] part Fermented product solution of Production Example 14 3.0
L-ascorbic acid-2-glucoside 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Sodium alginate 0.1
Potassium hydroxide Appropriate amount Perfume Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 18 Pressed powder [A component] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
6-Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Fermented product powder of Production Example 15 0.1
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Fragrance Appropriate Amounts The above A component and B component were mixed and stirred, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 19. Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Fermented product solution of Production Example 7 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 20. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Fermented product solution of Production Example 8 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 21. Hair artic [component A] part ethanol 60.0
l-Menthol 0.5
Fragrance 0.1
Methylparaben 0.1
[B component]
Glycerin 2.0
1,3-butylene glycol 2.0
Fermented product solution of Production Example 10.0 10.0
Purified water An amount that makes the total amount 100 parts The above A component and B component were each dissolved at room temperature, and then the B component was added to the A component with stirring and dissolved to obtain a hair artic.

Example 22. Hair treatment [ingredient] part stearyltrimethylammonium chloride 6.0
Polyvinylpyrrolidone 4.0
Glycerin 1.0
Ethylparaben 0.1
Fermented product solution of Production Example 10 5.0
Purified water in a total amount of 100 parts The above ingredients were heated to 80 ° C and then mixed and stirred to obtain a hair treatment.
This product was also suitable as a hair pack.

Example 23. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Fermented product solution of Production Example 11 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a hair shampoo It was.

Example 24. Hair rinse [component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Fermented product solution of Production Example 12 5.0
1,3-butylene glycol 5.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain hair rinse It was.

Example 25. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Fermented product solution of Production Example 13 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 26. Soap [component A] part hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Fermented product powder of Production Example 15 0.5
Purified water Amount of 100 parts [component C]
Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Test Example 1 Degranulation suppression test The fermented product solution obtained in Production Example 1 was examined for its degranulation inhibitory effect on basophils.
[Test method]
(A) Induction of degranulation into cell culture supernatant Rat basophil leukemia cells (RBL-2H3: Lot. 408828 (7)) are suspended in a 96-well plate in Eagle's minimum essential medium containing 10% (NCS). 1 × 10 ⁵ seeds were inoculated and cultured at 37 ° C. for 24 hours. The confluent cells were released into a releasing buffer [117 mM NaCl, 5.4 mM KCl, 2.0 mM CaCl2, 0.8 mM MgSO4, 5.6 mM D-glucose, 25 mM HEPES, 1 mg / mL BSA / pH 7.7] 200 μL / well The fermented product solution of Production Example 1 was added to each well so as to have a concentration of 1.0% or 2.0% (as a solution concentration). Moreover, it replaced with the fermented material solution of manufacture example 1, and provided the test plot which added releasing buffer buffer as control. In order to induce degranulation, 200 μg / mL compound 48/80 / releasing buffer was added and incubated at 37 ° C. for 1 hour. At the same time, a test group that did not induce degranulation by adding a releasing buffer not containing compound 48/80 was set as a control.
(B) Determination of degranulation rate by measuring β-hexosaminidase (β-hexosaminidase) activity In order to measure the enzyme activity of β-hexosaminidase released extracellularly after degranulation induction, 50 μL of cell supernatant Was dispensed into another 96-well microplate. β-hexosaminidase activity was measured as follows.
5 μm p-nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside (p-Nitrophenyl-2-acetamide-2-deoxy-β-D-glucopyranoside) as a substrate in 50 μL of each cell supernatant taken on a separate plate Was added, and the mixture was reacted in a CO 2 incubator at 37 ° C. for 30 minutes. Thereafter, 100 μL of 0.2 M glycine buffer (pH 10.7) was added to stop the reaction, and the absorbance at 415 nm was measured with an absorbance plate reader, which was used as an index of β-hexosaminidase activity.

[result]
The results are shown in FIG. In FIG. 1, A, B, C, and D are the control group, the control group, the fermentation product solution 1.0% addition group of Production Example 1 and the fermentation product solution 2.0% addition group of Production Example 1, respectively. The measurement results are shown. In FIG. 1, the larger the absorbance value at 415 nm, the stronger the activity of β-hexosaminidase, which means that degranulation is remarkable.
As shown in FIG. 1, it is recognized that the yeast fermented solution of the gold needle vegetable of the Liliaceae forget-me-not plant (fermented product solution of Production Example 1) strongly suppresses the release (degranulation) of chemical mediators from basophils. It is done.

Test Example 2 Prostaglandin E2 Production Inhibition Test For the fermented product solution obtained in Production Example 1, the production inhibitory action of prostaglandin E2 was examined using rabbit cornea-derived cells.
[Test method]
(A) Induction of degranulation into cell culture supernatant Rabbit cornea-derived cells (SIRC: Lot. 409916 (7)) were suspended in 10% (FBS) -containing Eagle's minimum essential medium and 5.0 × in a 96-well plate. After seeding 10 ³ pieces at a time and culturing at 37 ° C. for 3 days, the fermented product solution of Production Example 1 was added to the medium so as to have a concentration of 1.0% (as a solution concentration), and further cultured for 24 hours. As a control, purified water was added instead of the fermented product solution of Production Example 1 and cultured for 24 hours. Next, an ultraviolet B wave of 0.5 mW / cm ² was irradiated from the bottom of the incubator, and after further culturing for 2 days, the amount of PGE2 secreted into the culture supernatant was measured using a PGE2 measurement kit (manufactured by Cayman Caymic). Measured. As a positive control, 10 μM indomethacin was used.
Further, as in the case of the control, purified water was added and cultured for 24 hours, and then a control group not irradiated with ultraviolet rays was provided.

[result]
The results are shown in FIG. In FIG. 2, E, F, G, and H show the results of the control group, the control group, the fermented product solution 1.0% addition group of Production Example 1, and the positive control group, respectively.
As shown in FIG. 2, the effect of suppressing the production of PGE2 was recognized in the yeast fermented solution of the gold needle vegetable belonging to the genus Forget-me-not, and it was found that the fermented product has an anti-inflammatory effect.

Test Example 3 Inhibition test of hyaluronic acid fragmentation by hydroxy radical The fermented product solution obtained in Production Example 1 was examined for its inhibitory action on the fragmentation of hyaluronic acid by hydroxy radical.
[Test method]
0.45 μL of 0.04% hyaluronic acid / phosphate buffer (pH 5.3) and 0.05 μL of the fermented product solution of Production Example 1 were mixed, and 0.15 μL of 5 mM aqueous hydrogen peroxide solution and 0.375 mM chloride were mixed therewith. 0.10 μL of ferrous aqueous solution was mixed and allowed to stand at 37 ° C. for 24 hours, and hydroxy radicals were generated by Fenton reaction. Thereafter, 20 μL of the reaction solution was taken, 200 μL of 0.1% albumin / acetic acid buffer (pH 3.75) was added thereto, and the turbidity of the solution was measured by absorbance at 630 nm. In this case, if hyaluronic acid is not fragmented, a complex with albumin is generated and the turbidity is high. On the other hand, if hyaluronic acid is fragmented by a hydroxy radical, the complex is not formed and the turbidity is low.
In addition, a section where no aqueous hydrogen peroxide solution and ferrous chloride are added is provided, and a section without fragmentation treatment is provided, and a section where purified water is added instead of the fermented product solution of Production Example 1 is provided to fragment hyaluronic acid. I let you.
The results are shown as hyaluronic acid residual ratios in the respective fragmentation-treated sections when the hyaluronic acid residual ratio in the non-fragmented section is defined as 100%.

[result]
The results are shown in FIG. In FIG. 3, I, J, and K indicate the results of the section without the fragmentation treatment, the section with the purified water added, and the section with the fermented product solution added in Production Example 1, respectively.
From the result of FIG. 3, it was confirmed that the fermented product solution of the present invention has an effect of suppressing the fragmentation of hyaluronic acid by hydroxy radicals.

Test Example 4 DPPH radical scavenging test With respect to the fermented product solution obtained in Production Example 1 (fermented product solution of Production Example 1), the DPPH radical scavenging action was examined.
[Test method]
After dissolving 2.4 parts of DPPH in 25 parts of ethanol, 50 parts of purified water was added to prepare a DPPH solution. To 24 parts of this DPPH solution, 19.2 parts of 18 v / v% ethanol solution and 4.8 parts of 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH addition solution. In addition, in order to subtract the influence of the color of the fermented product solution on the test, a solution mixed with 18 v / v% ethanol solution and 2 M acetic acid-sodium acetate buffer solution using 50 v / v% ethanol solution instead of DPPH solution. Was used as a control solution.
A solution prepared by diluting the fermented product solution of Production Example 1 with purified water to 1.0% or 2.0% (as a solution concentration) is used as a test solution, and this test solution and a DPPH-added solution or a control solution are 1 : After mixing at a ratio of 3 and allowing to stand at 37 ° C. for 20 minutes, the absorbance at 550 nm when each test solution was mixed with the DPPH-added solution, and the absorbance at 550 nm when each test solution was also mixed with the control solution The residual amount of DPPH radicals was confirmed.
At the same time, a test using purified water instead of the fermented product solution of Production Example 1 was also conducted as a control.
The results are shown as the residual ratio when the control DPPH radical residual ratio is 100%.

[result]
The results are shown in FIG. In FIG. 4, L, M, and N show the results of the control, 1.0% addition of the fermented product solution of Production Example 1 and 2.0% addition of the fermented product solution of Production Example 1, respectively.
As shown in FIG. 4, the fermented product solution of the present invention was confirmed to have an effect of scavenging DPPH radicals.

Test Example 5 Skin irritation [sample]
(1) Kneaded fermented product solution of Production Example 1 with JP Viscous Vaseline to a concentration of 5% (invention sample)
(2) JP Hydrophilic Petrolatum (control)

[Test method]
Five adult males aged 20 to 50 years were used as subjects, and the inner side of each upper arm was wiped with ethanol to remove sebum, and the sample of the present invention and a control JP hydrophilic petrolatum were applied to the aluminum plate of the fin chamber. Each applied to 0.2 g was pasted. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
After 1 hour, 24 hours and 48 hours after removing the patch, the state of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by dreze method”, and the average value of 5 subjects was calculated. Asked.
(Erythema)
Score Skin condition 0: No erythema 1: Extremely mild erythema 2: Clear erythema 3: Moderate to strong erythema 4: Light crust formation in deep crimson erythema (edema)
Score skin condition 0: No edema 1: Extremely mild edema 2: Clear edema (clearly distinguishable from surroundings)
3: Moderate edema (swelling of 1 mm or more)
4: Strong edema (further spread around)

[result]
The results are shown in Table 1.

  As is apparent from the results of Table 1, the fermented product solution of the present invention has almost no skin irritation and is extremely safe.

These are the graphs which show the β-hexosaminidase activity (absorbance at 415 nm) in each test section of Test Example 1. (Vertical axis: absorbance at 415 nm) These are the graphs which show the production amount of prostaglandin E2 in each test section of Test Example 2. (Vertical axis: Prostaglandin E2 amount (pg / mL)) These are graphs showing the hyaluronic acid residual rate in each test section of Test Example 3. (Vertical axis: Hyaluronic acid residual rate (%)) These are the graphs which show the DPPH radical residual rate at the time of each sample addition of Test Example 4. (Vertical axis: DPPH radical residual rate (%))

Explanation of symbols

A Control group in Test Example 1 B Control group in Test Example 1 C 1.0% fermented product solution of Production Example 1 in Test Example 1 D Fermentation of Production Example 1 in Test Example 1 2.0% addition of product solution E Target group in Test Example 2 F Control group in Test Example 2 G 1.0% fermented product solution of Production Example 1 in Test Example 2 H Test Example 2 In the positive control section I In the test example 3 No fragmentation section J In the purified water addition section in the test example 3 K In the test example 3 the fermented product solution addition section L In the test example 4 Control M Addition of 1.0% Fermentation Solution from Production Example 1 in Test Example 4 N Addition of 2.0% Fermentation Solution from Production Example 1 in Test Example 4

Claims (7)

A cosmetic comprising a fermented product obtained by fermenting a lilyaceae plant and / or an extract thereof with yeast. The cosmetic according to claim 1, wherein a gold needle vegetable which is a crude drug is used as a plant belonging to the genus Forget-me-not. The cosmetic according to claim 1, wherein hemlock (Hemerocallis fulva) is used as a plant belonging to the genus Forget-me-not. The cosmetic according to claim 3, wherein a cocoon is used. The cosmetic according to claim 1, wherein Saccharomyces cerevisiae is used as the yeast. A lily family Forget-me-not plant and / or an extract thereof is hydrolyzed with at least one enzyme selected from a proteolytic enzyme, a amylolytic enzyme and a fibrinolytic enzyme before and / or during the fermentation of yeast. 1. Cosmetics according to 1. The cosmetic according to claim 6, wherein three kinds of enzymes, a proteolytic enzyme, a amylolytic enzyme, and a fibrinolytic enzyme, are used in combination.