JP6723679B2 - Cosmetics - Google Patents

Description

The present invention relates to a functional material which has excellent biosafety and is incorporated into cosmetics (including quasi drugs) and oral cosmetic compositions.

Skin has internal factors such as inactivation of cell proliferation and differentiation with age, decrease of hormone secretion, decrease in amount of extracellular matrix components, active oxygen induced by sunlight (ultraviolet rays), and air pollutants. And chemical substances such as environmental hormones, allergens such as pollen, and external factors such as environmental stress are intricately intertwined with each other, causing aging and rough skin, and changing the color tone. Among these, external factors such as ultraviolet rays, chemical substances, and allergens may damage cells and tissues of the skin and alter biological components, and as a result, they may be factors that generate antigens in the skin. is there. From these facts, these external factors cause various factors such as inflammation of the skin and development of allergies due to the antigen, and further induce abnormal deposition of melanin pigment to cause spots, freckles, melasma, etc. Inflicts damage.

In order to prevent various skin troubles caused by the above factors and keep the skin in a healthy and youthful state, conventionally, use of various active ingredients has been proposed, and cosmetics and pharmaceutical departments containing these active ingredients have been proposed. Foreign products are on the market. For example, whitening agents such as kojic acid, arbutin and vitamin Cs; antioxidants such as vitamin Es; anti-inflammatory agents such as glycyrrhizinic acid; various UV absorbers; α-hydroxycarboxylic acid, placental extract, γ-amino Cell-activating components such as -β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin and hyaluronic acid; moisturizers such as urea have been proposed. However, with conventional active ingredients, it is difficult to sufficiently satisfy the efficacy and biosafety, and there is a demand for a functional raw material in which this point is improved.

In view of such problems of the prior art, the present inventors have conducted earnest research to find out new active ingredients derived from natural products from the viewpoint of biosafety. As a result, the extract of sprout of sunflower, which is a plant of the genus Sunflower of the Asteraceae family, has an excellent whitening effect, an anti-aging effect and a rough skin improving effect, and by incorporating the extract, it is excellent in biosafety and efficacy. It has been found that it becomes possible to provide cosmetics and cosmetic oral compositions.

Heretofore, it has been known to incorporate sunflower stem, leaf, seed and petal extracts into cosmetics (Patent Documents 1 to 4). However, sunflower sprout extract has a whitening effect, an anti-aging effect and a rough skin improving effect, and by incorporating this, it is possible to provide a cosmetic composition and a beauty oral composition having excellent efficacy. Nothing was known.

JP 57-054110 JP 2000-351723 JP 2001-048773 A JP 2002-226322 A

The present invention is a whitening agent containing as an active ingredient an extract of sprout of sunflower (Helianthus annuus) of the sunflower (Helianthus) genus (Asteraceae).
The present invention is an anti-aging agent containing, as an active ingredient, an extract of sprouts of sunflower (Helianthus annuus) belonging to the genus Sunflower (Asteraceae).
The present invention is a rough skin improving agent containing an extract of sprouts of sunflower (Helianthus annuus) of the sunflower (Helianthus) genus of the family Asteraceae as an active ingredient.
The present invention is a cosmetic containing an extract of sprout of sunflower (Helianthus annuus) of the sunflower (Helianthus) genus (Asteraceae) as an active ingredient.

ADVANTAGE OF THE INVENTION According to this invention, the compounding agent of the outstanding cosmetics or oral composition for cosmetics can be provided by the whitening effect, anti-aging effect, and rough skin improving effect which the extract of the sunflower sprout which is an active ingredient has. In addition, since the extract is derived from a natural product, it is possible to provide a cosmetic composition or a cosmetic oral composition having excellent biosafety.

Hereinafter, the present invention will be described in detail. In the present specification, the term "cosmetics" is used in a broad sense to include not only so-called cosmetics but also quasi drugs.

The present invention uses, as an active ingredient, an extract of sprout of sunflower (Helianthus annuus) belonging to the genus Sunflower (Helianthus) of the family Asteraceae. Here, the "sprout" refers to a young sunflower, and preferably has 2 to 10 leaves. The size of the "sprout" of sunflower used in the present invention is preferably 5 cm to 20 cm.

The sunflower sprouts used for the preparation of the extract may be fresh or dried. In addition, before the extraction step, the shoots may be pasty, shredded or crushed, if necessary. The extraction can be performed by bringing the sunflower sprouts into contact with the extraction solvent based on a conventional method such as a dipping method, a supercritical method, or a steam distillation method.

As the extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Examples include esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, and chloroform, which may be used alone or in admixture of two or more. Used.

Among these extraction solvents, the whitening action of the obtained extract, anti-aging action, rough skin improving action, further, from the viewpoint of skin irritation, and also from the viewpoint that it can be widely applied to cosmetics and the like, In the present invention, hydrophilic solvents such as water, lower alcohols or polyhydric alcohols are suitable. Preferable examples of the case where this hydrophilic solvent is used include, for example, water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) used alone, or water and lower alcohols. The use of a mixed solvent with (particularly ethanol) or a mixed solvent with water and a polyhydric alcohol (particularly 1,3-butylene glycol, glycerin) and the like can be mentioned. Among them, water alone or water and 1,3 -A mixed solvent of butylene glycol is particularly preferred.

When a mixed solvent is used, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1:10 to 20:1, and the mixed solvent of water and ethanol is If so, it is preferably in the range of 1:10 to 25:1, and in the case of a mixed solvent of water and glycerin, it is preferably in the range of 1:10 to 20:1.

The weight ratio of the dry part of sunflower "sprout" to the extraction solvent is preferably in the range of 1:1 to 1:50, more preferably in the range of 1:5 to 1:35.

The pH of the extract to be prepared is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent may be mixed with an alkaline modifier such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic modifier such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid to obtain a desired amount. You may adjust so that it may become pH.

The extraction conditions such as the extraction temperature and the extraction time vary depending on the type and pH of the solvent used, but, for example, when water or 1,3-butylene glycol or a mixed liquid of water and 1,3-butylene glycol is used as the solvent. Then, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably 1 hour to 1 week, more preferably 4 hours. ~ 3 days range.

The extract obtained by the above method may be subjected to activated carbon treatment, resin treatment, ultrafiltration treatment or the like for the purpose of improving stability or the like.

The extract obtained under the above conditions generally has a pH adjusted to 4 to 8 and can be used as it is as a compounding agent for cosmetics and oral compositions for beauty treatments even if it is used at a desired concentration by vacuum concentration or the like. You may do it.

Examples of cosmetics (including quasi drugs) containing the sunflower sprout extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, and makeup presses. Examples include makeup cosmetics such as powders, cheeks, and white powders, facial cleansers, cleansing cosmetics such as body shampoos and soaps, hair cosmetics such as shampoos, hair conditioners, and hair creams, and bath agents. Of course, it is not limited to these. Oral compositions for beauty include beverages such as beauty drinks, nutritional drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products, jellies, candy. , Foods such as tablet confections, gums, etc.; tablets, liquids, granular or jelly-like health foods/beverages, etc. can be blended, but the present invention is not limited to this, and can be blended into foods and drinks that can be taken orally can do.

The amount of sunflower sprout extract in cosmetics is generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight, in the case of basic cosmetics, as the solid content of the extract. In the range of 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight in the case of makeup cosmetics, and 0.002 to 0.002% in the case of cleaning cosmetics. The range is 10.0% by weight, preferably 0.02-7.0% by weight. Further, in the case of a cosmetic for hair, the solid content of the extract is generally 0.00001 to 5.0% by weight (solid content% by weight, hereinafter the same), and preferably 0.0001 to 3%. It is 0.0% by weight. The amount of the sunflower sprout extract in the cosmetic oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

In the cosmetic or cosmetic oral composition, in addition to the extract of sunflower sprouts, which is an essential component, or the extract and a whitening agent, components that are usually used in cosmetics, such as an oily component and a surfactant (synthetic system) are used. , Natural products), emulsifiers or emulsification aids, whitening agents, moisturizers, thickeners, antiseptic/bactericidal agents, powder components, ultraviolet absorbers, antioxidants, pigments, and fragrances, etc., if necessary. It can be blended. Further, as long as the effectiveness and characteristics of the sunflower sprout extract are not impaired, other physiologically active ingredients may be combined and blended.

Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadow foam oil, shea butter, tea tree oil, avocado oil, Plant-derived oils and fats such as macadamia nut oil, yuzu seed oil, citrus-derived oil, and plant-derived squalane; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; Hydrocarbons such as paraffin, petrolatum, paraffin wax, squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher grades such as lauryl alcohol, cetanol, stearyl alcohol Alcohols; synthetic esters such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.), synthetic triglycerides and the like can be mentioned.

As the surfactant, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkylphenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, tris Cationic surfactants such as alkylbenzyl ammonium salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethyl imidazolinium salts, N,N-dialkyl morpholinium salts, polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N'- An amphoteric surfactant such as β-hydroxypropylammoniosulfobetaine can be used.

As the emulsifier or emulsification aid, a stevia derivative such as enzyme-treated stevia, saponin or a derivative thereof, casein or a salt thereof (sodium, etc.), a complex of sugar and protein, sucrose or an ester thereof, lactose, a soybean-derived Water-soluble polysaccharides, soybean-derived protein-polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and Derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacterium fermented rice, lactic acid bacterium fermented germinated rice, lactic acid bacterium fermented cereals (wheat, beans, millet, etc.) and the like can also be blended.

Further, as a whitening agent, ascorbic acid and its derivative, hydroquinone or its derivative, kojic acid and its derivative, ellagic acid and its derivative, t-cycloamino acid derivative, nicotinic acid and its derivative, resorcinol derivative, tranexamic acid and its derivative. , 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhododenol, 4-(4-hydroxyphenyl)-4-butanol)), hydroxy Examples thereof include benzoic acid and its derivative, vitamin E and its derivative, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate). These may be blended alone or in combination of two or more. good.

Examples of the ascorbic acid derivative include sodium L-ascorbic acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium L-ascorbic acid-2-sulfate, and L-ascorbic acid-2-sulfate. Ascorbic acid ester salts such as ester magnesium, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside and L-ascorbic acid-5-glucoside, and the 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl group , Octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitic acid ester, L-ascorbic acid tetralauric acid ester and other L-ascorbic acid tetrafatty acid esters, 3-O-ethylascorbic acid, L-ascorbic acid 2-sodium phosphate-6-O-palmitate, glyceryl ascorbic acid or its acylated derivatives, glyceryl ascorbic acid derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbic acid, hyaluronic acid L-ascorbic acid Examples include derivatives. Examples of the idroquinone derivative include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of kojic acid derivatives include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid esters such as kojic acid dibutyrate, kojic acid ethers, and kojic acid saccharides such as kojic acid glucoside. Is mentioned. Examples of the tranexamic acid derivative include tranexamic acid esters (eg, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or salts thereof), and tranexamic acid amides (eg, tranexamic acid methylamide). Further, examples of the resorcinol derivative include 4-n-butylresorcinol, 4-isoamylresorcinol and the like. Examples of the 2,5-dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide and benzyl nicotinate. Examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like.

Moisturizers include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose and mucopolysaccharides (for example, hyaluronic acid). Acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (Hakuo) Examples include extracts, various amino acids and their derivatives.

Examples of the thickener include brown algae such as alginic acid, agar, carrageenan, and fucoidan, components derived from green algae or red algae; silane root (white and white) extracts; pectin, locust bean gum, aloe polysaccharides, polysaccharides produced by Alcaligenes, and the like. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxy vinyl polymer, acrylic acid/methacrylic acid copolymers; hyaluronic acid And derivatives thereof; polyglutamic acid and derivatives thereof and the like.

Examples of antiseptic/bactericidal agents include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophene, chlorhexidine hydrochloride, benza chloride. There are ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodinylurea), 1,2-pentanediol, various essential oils, bark distillate, radish fermentation broth and the like.

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic acid anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, adzuki beans, etc.) powder, etc.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), peony extract, silane root (white oak) extract, etc. There is.

As the physiologically active component, as a whitening component or an anti-aging component, for example, a placenta extract, a sophoraceae extract, a Yukinoshita extract, a perilla extract, a white mustard extract or a hydrolyzate thereof, a fermented product of white mustard, a damask rose Extract, peony extract or its hydrolyzate, lactic acid bacteria fermented rice, lotus seed extract or its hydrolyzate, lotus seed fermented product, ginseng extract, pearl barley hydrolyzate, pearl barley seed fermented product, royal jelly fermented product, sake lees Fermented product, Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, perilla extract, kiwi extract, chamomile extract, coral grass extract, rice leaf Extract or its hydrolyzate, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, seaweed extract such as caterpillar, extraction of marine flowering plants such as eelgrass Products, fermented soy milk, jellyfish water, rice fermentation extract, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (Dipotassium salt etc.), t-cycloamino acid derivative, vitamin A and its derivative, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, aloe extract, There are Mitsuishikonbu extract, sardine extract, Zizyphus joazeiro extract, immature peach extract and the like.

Next, the present invention will be described in more detail with reference to production examples, formulation examples and test examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means% by weight.

Production Example 1. Preparation of sunflower sprout extract Sunflower sprouts were dried, 540 g of purified water was added to 36 g of the dried product, and the mixture was immersed at 4° C., and then 540 g of 1,3-butylene glycol was added. This was extracted at 4° C. and then filtered to obtain 850 g of a transparent transparent sunflower sprout extract (solid content concentration 1.0%).

Production example 2. Preparation of sunflower sprout extract Sunflower sprouts were dried, 540 g of purified water was added to 36 g of the dried product, and the mixture was immersed at 4° C., and then 540 g of 1,3-butylene glycol was added. This is extracted at 4° C. and then filtered, and 850 g of the obtained liquid is added with 1.0% of a synthetic adsorbent and stirred for 1 hour. The synthetic adsorbent was removed by filtration to obtain 820 g of a light brown transparent sunflower sprout extract (solid content concentration 0.9%).

Comparative Production Example 1. Preparation of sunflower petal extract Sunflower petals were dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4° C., and then 60 g of 1,3-butylene glycol was added. This was extracted at 4° C. and then filtered to obtain 100 g of a light yellowish-brown transparent sunflower petal extract (solid content concentration 1.3%).

Comparative Production Example 2. Preparation of sunflower stalk extract Sunflower stalks were dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4°C, and then 60 g of 1,3-butylene glycol was added. This was extracted at 4° C. and then filtered to obtain 93 g of a light yellow transparent sunflower stem extract (solid content concentration 0.7%).

Comparative Production Example 3. Preparation of Sunflower Root Extract Sunflower root was dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4° C., and then 60 g of 1,3-butylene glycol was added. This was extracted at 4° C. and then filtered to obtain 95 g of a light yellow transparent sunflower root extract (solid content concentration: 0.3%).

Comparative Production Example 4. Preparation of sunflower leaf extract Sunflower leaves were dried, 60 g of purified water was added to 4 g of the dried product, and the mixture was immersed at 4°C, and then 60 g of 1,3-butylene glycol was added. This was extracted at 4° C. and then filtered to obtain 97 g of a transparent brown sunflower leaf extract (solid content concentration 0.5%).

Prescription example 1. Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B Component] Part 5.0 Extract Solution of Production Example 1
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Suitable amount Purified water Total amount of 100 parts [C component]
Fragrance Appropriate amount Each of the components A and B was heated to 80° C. or higher, then the component B was added to the component A, and the mixture was stirred, and further homogenized with a hyscotron (5000 rpm) for 2 minutes. After cooling this to 50° C., component C was added and mixed with stirring, and further cooled to 30° C. or lower to obtain a lotion.

Prescription example 2. Lotion A lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution in Production Example 2 was used in place of the extract solution in Production Example 1 contained in the component B of Formulation Example 1. ..

Prescription example 3. Emulsion [A component] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Extract Solution of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water 100 parts [C component]
Fragrances Appropriate amount Each of the above-mentioned components A and B was heated to 80° C. or higher, and then mixed with stirring. After cooling this to 50° C., component C was added and further stirred and mixed to obtain an emulsion.

Prescription example 4. Emulsion A milky lotion was prepared in the same manner as in Formulation Example 10 except that 2.0 parts of L-ascorbic acid-2-glucoside and 3.0 parts of arbutin were used instead of 2.0 parts of L-ascorbic acid-2-glucoside in Component B of Formulation Example 3. Obtained.

Prescription example 5. Emulsion An emulsion as in Formulation Example 10 except that 2.0 parts of tranexamic acid was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Formulation Example 3. Got

Prescription example 6. Emulsion In the same manner as in Formulation Example 10 except that 2.0 parts of L-ascorbic acid-2-glucoside and 3.0 parts of potassium hydroxide were used in Component B of Formulation Example 3 instead of 3.0 parts of nicotinic acid amide. An emulsion was obtained.

Prescription example 7. Emulsion [A component] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B Component] Part 5.0 Extract Solution of Production Example 1
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water 100 parts

Prescription example 8. Lotion [ingredient] part Extraction solution of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxy vinyl polymer 0.1
Fragrance Suitable amount Potassium hydroxide Suitable amount Purified water Amount such that the total amount becomes 100 parts The above ingredients were mixed to obtain a lotion.

Prescription example 9. Essence [ingredient] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract Solution of Production Example 2 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water Total amount is 100 parts After dissolving hyaluronic acid in purified water, the remaining raw materials were sequentially added and dissolved with stirring to obtain a transparent essence.

Prescription example 10. Essence An essence was obtained in the same manner as in Formulation Example 8 except that 5.0 parts of the extract solution in Production Example 2 was used instead of the extract solution in Production Example 1 in the components of Formulation Example 8.

Example 11. Liquid foundation [A component] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B Component] Part 5.0 Extract Solution of Production Example 1
Carboxymethyl cellulose sodium 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Total amount is 100 parts [C component] part Titanium oxide 8.0
Talc 4.0
Coloring Pigments Appropriate amounts The above-mentioned components A and B were each heated, and then mixed and stirred. This was reheated, the above-mentioned C component was added, and the mixture was poured into a mold and stirred until it reached room temperature to obtain a liquid foundation.

Prescription example 12. Body shampoo [A component] part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium liquid (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B Component] Part 5.0 Extract Solution of Production Example 1
1,3-butylene glycol 2.0
Purified water Total amount is 100 parts A component and B component are respectively heated to 80° C. and uniformly dissolved, then B component is added to A component, and stirring is continued and cooled to room temperature to obtain a body shampoo. It was

Prescription example 13. Hair growth cosmetics [ingredient] part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-menthol 0.8
Tamasaki Tsuduji Root Extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract solution of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine proper amount ethanol 20
Amount of purified water to be 100 parts The above ingredients were thoroughly mixed with stirring to obtain a hair-growth agent.

Prescription example 14. Hair shampoo [A component] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) alkyl ether sodium sulfate 20.0
Lauryl dimethylamino acetic acid betaine 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract solution of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount is 100 parts Component A and component B are each heated to 80°C to dissolve them uniformly, then component B is added to component A and stirring is continued to cool to room temperature to obtain a hair shampoo. It was

Example 15. Hair conditioner [A component] part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyl dimethyl ammonium chloride 1.5
Stearyl trimethyl ammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B Component] Part 2.0 Extract Solution of Production Example 1
1,3-butylene glycol 5.0
Purified water Total amount of 100 parts A component and B component were respectively heated to 80° C. and uniformly dissolved, then B component was added to A component, and stirring was continued to cool to room temperature to obtain hair rinse. ..

Prescription example 16. Beverage [ingredient] part Extraction solution of Production Example 1 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (vitamin C) 0.01
Amount of purified water 100 parts

Prescription example 17. Tablet [ingredient] part Extract of Production Example 2 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After mixing the above parts by weight of the respective components, pressure molding was carried out to obtain tablets.

Test Example 1. Evaluation of melanin synthesis inhibition <Experimental method>
B16-F10 mouse melanoma cells were prepared at a concentration of 1×10 ⁵ cells/mL in 10% FBS-containing minimal Eagle's essential medium (MEM), and seeded in a 6 mmφ petri dish in an amount of 1 mL. Cultured at °C. After 24 hours, a culture solution containing the extract of Production Example 1 and 1 mM theophylline was additionally added, and the cells were further cultured for 72 hours. Here, the extract of Production Example 1 was prepared so that the final concentration was 1.0% as a solution with respect to the total amount of the culture solution. In addition, as a control, a test section was set in which a culture solution containing 50% 1,3-butylene glycol was additionally added instead of the extract of Production Example 1. Further, as a comparative control of the effect, a test section was set in which the culture solution containing each extract of Comparative Production Examples 1 to 4 was additionally added so that the final concentration was 1% instead of the extract of Production Example 1. .. After the culture was completed, the supernatant was removed and the cells were collected using trypsin. A melanin solution (10% dimethyl sulfoxide-containing 1N sodium hydroxide aqueous solution) was added to the collected cells, and then boiled for 10 minutes to obtain a cell lysate. The value of the absorbance of the cell lysate at 490 nm measured using a light absorption plate reader (Model 680, manufactured by Bio-Rad) was defined as the amount of melanin. After measuring the amount of melanin, the amount of protein in the cell lysate was measured and the amount of melanin synthesis per amount of protein was calculated.

The results of Test Example 1 are shown in Table 1.
[Table 1]

As shown in Table 1, the extract of Production Example 1 according to the present invention showed a markedly excellent melanin production inhibitory effect, which was compared with other parts of sunflower (flower, stem, root, leaf). And was excellent.

Test example 2. SOD-like action evaluation test <Experimental method>
0.2 M Tris-HCl buffer 50 μL, 1 mM ethylenediaminetetraacetic acid (EDTA) disodium solution 20 μL, 0.75 mM nitroblue tetrazolium chloride (NBT) solution 10 μL, 1 mM xanthine solution 20 μL, 0.06 U/mL xanthine oxidase solution 50 μL, production A sample solution was prepared by mixing 50 μL of the extract of Example 1. Here, two kinds of sample solutions were prepared so that the final concentration of the extract of Production Example 1 as a solution was 0.5% and 1.0%, respectively, with respect to the total amount. This sample solution was incubated at 37° C. for 5 minutes to generate superoxide. Then, the amount of formazan produced by the reduction of NBT with superoxide was measured for the absorbance at 560 nm using an absorption plate reader (Model 680, manufactured by Bio-Rad). Further, as a control, 50% 1,3-butylene glycol solution was used in place of the extract of Production Example 1 to perform the same operation as above, and the absorbance at the time of adding each sample to the absorbance obtained here. The relative value of was calculated|required and it was made into the superoxide residual rate (%). Further, instead of the extract of Production Example 1 as a comparative control, the extract of Comparative Production Examples 1 to 4 (prepared to have final concentrations of 0.5% and 1.0% as a solution) is included. The same operation as above was performed using the sample solution to compare the effects. In addition, in order to confirm whether the test system is functioning normally, the same test was performed in the case where superoxide dismutase (SOD) 8.75 U/mL was added as a positive control instead of the sample solution. ..

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, it was revealed that the extract according to the present invention exhibits markedly superior SOD-like activity as compared with other parts (flower, stem, root, leaf).

Test example 3. Evaluation test for suppression of oxidative damage in fibroblasts <Experimental method>
Human dermis-derived fibroblasts (NB1RGB) were prepared at 2×10 ⁵ cells/mL in 0.5% NCS-containing minimal essential medium, and 100 μL was seeded on a 96-well microplate and saturated with 5% carbon dioxide gas. Culture was performed at 37° C. under steam. After 24 hours, a culture solution containing the extract of Production Example 1 was additionally added and cultured. Here, two types of sample solutions were prepared so that the final concentration of the extract of Production Example 1 as a solution was 0.5% and 1.0%, respectively, with respect to the total amount. Further, a test section to which a culture solution containing 50% 1,3-butylene glycol was additionally added so that the final concentration was 1.0% was set as a control. Further, instead of the extract of Production Example 1 as a comparative control, the extract of Comparative Production Examples 1 to 4 (prepared to have final concentrations of 0.5% and 1.0% as a solution) is included. A test section (comparative section) to which the sample solution was added was set. After 48 hours, the supernatant of the test section was removed, 10 μM of 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-2DA) was introduced into the cells, and then UV was irradiated. After UV irradiation, the supernatant was removed and 1% Triron X-100 was added to obtain a cell lysate. The fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) of the cell lysate was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was taken as the amount of oxidative damage. After the measurement, the amount of protein in the cell lysate was measured, and the amount of oxidative damage per amount of protein was calculated.

[Table 3]

As shown in Table 3, the extract of Production Example 1 according to the present invention is markedly superior to the extracts of other parts (flower, stem, root, leaf) in suppressing oxidative damage in fibroblasts. Showed the effect.

Due to the above effects, cosmetics and cosmetic oral composition containing the extract according to the present invention, skin spots, freckles, prevent pigment pigmentation such as liver spots, improve, also, ultraviolet rays, air pollutants, It is possible to protect the skin from oxidative damage caused by allergens such as pollen, and to improve the rough skin, the aging of the skin, etc. due to those causes.

Claims (2)

A whitening agent containing, as an active ingredient, an extract of a mixed solvent of water and 1,3-butylene glycol (excluding an extract containing cinnaline) of sprouts of sunflower (Helianthus annuus) of the sunflower (Helianthus) genus (Asteraceae). A whitening cosmetic composition containing an extract of a mixed solvent of water and 1,3-butylene glycol (excluding an extract containing cinnaline) of sprouts of sunflower (Helianthus annuus) of the sunflower (Helianthus) genus (Asteraceae) ..