JP2016000706A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a novel functional material for cosmetics (for whitening, anti-aging action, rough and dry skin improving action) and for health promotion which has naturally derived components and is excellent in biosafety.SOLUTION: The invention relates to a whitening agent, anti-aging agent, rough and dry skin improving agent, and cosmetics containing such agents, or an oral composition for cosmetics which contain sprout extract of Helianthus annuus of Asteraceae Helianthus as an active ingredient. The extract and related compositions prevent and improve pigmentation, such as stains, freckles, and chloasma of the skin, and protect the skin from the oxidation damage caused by allergen, such as ultraviolet, air contaminants, pollen, and the like, and further exhibit an effect of improving the rough and dry skin, dermal aging, or the like due to such causes.

Description

  The present invention relates to a functional material excellent in biological safety and blended in cosmetics (including quasi-drugs) and oral cosmetic compositions.

  Skin has internal factors such as inactivation of cell growth / differentiation with age, decreased hormone secretion, quantitative decrease in extracellular matrix components, active oxygen induced by sunlight (ultraviolet rays), air pollutants Chemical substances such as environmental hormones, allergens such as pollen, and external factors such as environmental stress are intertwined in a complex manner, resulting in aging and rough skin, and color changes. Among them, ultraviolet rays, chemical substances, and allergens, which are external factors, can damage skin cells and tissues and alter biological components, resulting in the generation of antigens in the skin. is there. Therefore, these external factors can cause skin inflammation and allergies due to antigens, and also cause abnormal deposition of melanin pigments to cause spots, buckwheat, liver spots, etc. Will cause damage.

  In order to prevent various skin troubles caused by the above factors and keep the skin healthy and youthful, the use of various active ingredients has been proposed in the past, and cosmetics and pharmaceutical departments containing these active ingredients have been proposed. Foreign goods are on the market. For example, whitening agents such as kojic acid, arbutin and vitamin C; antioxidants such as vitamin E; anti-inflammatory agents such as glycyrrhizic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract, γ-amino Cell activation components such as β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, and hyaluronic acid; and humectants such as urea have been proposed. However, it is difficult for conventional active ingredients to sufficiently satisfy efficacy and biosafety, and there is a demand for functional raw materials with improved such points.

  In view of the problems of the prior art, the present inventors have conducted intensive research to find a new active ingredient derived from a natural product from the viewpoint of biological safety. As a result, an extract of a shoot of sunflower, a plant belonging to the genus Sunflower family, exhibits excellent whitening action, anti-aging action, and skin roughening action, and it is excellent in biological safety and effectiveness by blending the extract. The present inventors have found that it is possible to provide cosmetics and cosmetic oral compositions.

  Conventionally, it has been known to blend sunflower stems, leaves, seeds, and petals extracts into cosmetics (Patent Documents 1 to 4). However, the sunflower sprout extract has a whitening effect, an anti-aging effect, and a rough skin improving effect, and by incorporating this, it is possible to provide a cosmetic or cosmetic oral composition with excellent effectiveness. Was not known at all.

JP 57-054110 JP 2000-351723 A JP2001-048773 JP 2002-226322 A

The present invention is a whitening agent comprising, as an active ingredient, an extract of a sprout of a sunflower (Helianthus annuus) of the genus Asteraceae (Helianthus).
The present invention is an anti-aging agent comprising, as an active ingredient, an extract of a sprout of a sunflower (Helianthus annuus) from the genus Asteraceae (Helianthus).
The present invention is a rough skin improving agent comprising, as an active ingredient, an extract of a sprout of a sunflower (Helianthus annuus) of the genus Asteraceae (Helianthus).
The present invention is a cosmetic comprising, as an active ingredient, an extract of a sprout of a sunflower (Helianthus annuus) from the genus Asteraceae (Helianthus).

  ADVANTAGE OF THE INVENTION According to this invention, the compounding of the cosmetics and the oral composition for cosmetics which were excellent by the whitening effect | action, anti-aging effect, and rough skin improvement effect which the extract of the sunflower bud which is an active ingredient has can be provided. In addition, since the extract is derived from a natural product, it can also provide a cosmetic or cosmetic oral composition excellent in biological safety.

  Hereinafter, the present invention will be described in detail. In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

  The present invention uses an extract of a bud of the sunflower (Helianthus annuus) of the asteraceae (Helianthus) as an active ingredient. Here, “sprout” refers to a juvenile sunflower and preferably has 2 to 10 leaves. In addition, the size of the “sprout” of the sunflower used in the present invention is preferably 5 cm to 20 cm.

  The sunflower sprouts used for the preparation of the extract may be raw or dried. Further, before the extraction step, the shoots may be pasted, chopped or pulverized as necessary. Extraction can be performed by bringing sunflower shoots into contact with an extraction solvent based on conventional methods such as immersion, supercritical methods, and steam distillation.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, the whitening action, anti-aging action, skin roughening action of the resulting extract, and also from the viewpoint of skin irritation, and also because it can be widely applied to cosmetics, etc. In the present invention, hydrophilic solvents such as water, lower alcohols and polyhydric alcohols are preferred. Preferable examples of using this hydrophilic solvent include, for example, water, lower alcohols (especially ethanol), or polyhydric alcohols (especially 1,3-butylene glycol) alone, or water and lower alcohols. Examples include the use of a mixed solvent with (especially ethanol) or a mixed solvent of water and a polyhydric alcohol (especially 1,3-butylene glycol, glycerin). Among them, water alone or water with 1,3 -A mixed solvent of butylene glycol is particularly preferred.

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1:10 to 20: 1, and the mixed solvent is water and ethanol. If it exists, it is preferable to set it as the range of 1: 10-20: 1 if it is a mixed solvent of 1: 10-25: 1 and water and glycerol.

  The weight ratio of the dried portion of sunflower “sprout” to the extraction solvent is preferably in the range of 1: 1 to 1:50, and more preferably in the range of 1: 5 to 1:35.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C to 80 ° C, more preferably in the range of 0 ° C to 20 ° C, and the extraction time is preferably 1 hour to 1 week, more preferably 4 hours. ~ 3 days range.

  The extract obtained by the above method may be subjected to activated carbon treatment, resin treatment, ultrafiltration treatment or the like for the purpose of improving stability or the like.

  The extract obtained under the above conditions is generally adjusted to a pH of 4 to 8, and even if it is used as it is as a compounding agent for cosmetics or oral cosmetic compositions, it is used as a desired concentration by vacuum concentration or the like. You may do it.

  Examples of cosmetics (including quasi-drugs) containing the sunflower sprout extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, and makeup presses. Makeup cosmetics such as powder, cheek red, white powder, cleansing cosmetics such as face wash, body shampoo, soap, hair cosmetics such as shampoo, hair conditioner, hair cream, and bath preparations. Of course, it is not limited to these. In addition, as cosmetic oral compositions, beverages such as beauty drinks, energy drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products, jelly, candy , Tablets, gums and other foods; tablets, liquids, granules or jelly-like health foods / beverages, etc., but the present invention is not limited to these, and can be incorporated into foods and drinks that can be taken orally can do.

  The amount of sunflower sprout extract in cosmetics is generally 0.002 to 1.0% by weight, preferably 0.02 to 0.2% by weight, in the case of basic cosmetics, as the solid content of the extract. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. It is in the range of 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0 wt% (solid content wt%, the same shall apply hereinafter), preferably 0.0001 to 3 0.0% by weight. Moreover, the blending amount of the extract of sunflower shoots in the cosmetic oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

  In cosmetic or cosmetic oral compositions, in addition to the essential sunflower shoot extract, or the extract and a whitening agent, components commonly used in cosmetics such as oily ingredients, surfactants (synthetic systems) , Natural products), emulsifiers or emulsifiers, whitening agents, moisturizers, thickeners, antiseptics / bactericides, powder components, UV absorbers, antioxidants, pigments, and fragrances as needed. Can be blended. Moreover, as long as the effectiveness and characteristics of the sunflower sprout extract are not impaired, it may be combined with other physiologically active ingredients.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Macadamia nut oil, yuzu seed oil, oils derived from citrus fruits, plant-derived fats and oils such as plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; Hydrocarbons such as paraffin, petrolatum, paraffin wax, squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher grades such as lauryl alcohol, cetanol, stearyl alcohol Alcohols; isopropyl myristate, pa Michin isopropyl, butyl oleate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, and soybeans. Water-soluble polysaccharides, soy-derived protein-polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and Derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.) and the like can also be blended.

  In addition, as a whitening agent, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, kojic acid and derivatives thereof, ellagic acid and derivatives thereof, t-cycloamino acid derivatives, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), hydroxy Examples include benzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate). These may be blended alone or in combination. good.

  Examples of ascorbic acid derivatives include L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, and L-ascorbic acid-2-sulfate. Ascorbic acid ester salts such as ester magnesium, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside and L-ascorbic acid-5-glucoside, acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is a hexanoyl group) L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid 2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acylated derivatives thereof, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid Etc. Alternatively, examples of idroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. Is mentioned. Examples of the tranexamic acid derivatives include tranexamic acid esters (for example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or salts thereof), and amides of tranexamic acid (for example, tranexamic acid methylamide). Moreover, as a resorcinol derivative, 4-n-butyl resorcinol, 4-isoamyl resorcinol etc. are mentioned, for example. Examples of the 2,5-dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, and the like. Examples of the nicotinic acid derivative include nicotinic acid amide and nicotinic acid benzyl. Examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Examples thereof include Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, dry bark, radish fermentation liquor and the like.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc.), peony extract, silane root (white) extract, etc. There is.

  Examples of physiologically active ingredients include whitening ingredients or anti-aging ingredients, such as placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, white coconut extract or hydrolyzate thereof, fermented white coconut, damask rose Extract, peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract, pearl hydrolyzed product, pearl seed fermented product, royal jelly fermented product, sake lees Fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, Perilla extract, Kiwi extract, Chamomile extract, Coral grass extract, Rice leaf Extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, catamenkiri Extracts of seaweeds such as rhinoceros, extracts of marine flowering plants such as sea cucumbers, fermented soymilk, jellyfish water, rice fermentation extract, linoleic acid and its derivatives or processed products (such as liposomal linoleic acid), animals or fish Collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid , Gentian extract, licorice extract, carrot extract, aloe extract, honey comb extract, anaosa extract, Zizyphus joazeiro extract, immature peach extract and the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of Sunflower Sprout Extract Sunflower sprouts were dried, 540 g of purified water was added to 36 g of the dried product and immersed at 4 ° C., and then 540 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 850 g of a brown transparent sunflower sprout extract (solid content concentration 1.0%).

Production Example 2 Preparation of sunflower sprout extract Sunflower sprouts were dried, 540 g of purified water was added to 36 g of the dried product and immersed at 4 ° C., and then 540 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered, and 1.0% of the synthetic adsorbent was added to 850 g of the resulting liquid and stirred for 1 hour. The synthetic adsorbent was removed by filtration to obtain 820 g of a light brown transparent sunflower sprout extract (solid content concentration 0.9%).

Comparative Production Example 1 Preparation of sunflower petal extract The sunflower petals were dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4 ° C., and then 60 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 100 g of a light yellowish-brown transparent sunflower petal extract (solid content concentration 1.3%).

Comparative Production Example 2 Preparation of sunflower stalk extract 60 g of purified water was added to 4 g of the dried product and 60 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 93 g of a light yellow transparent sunflower stalk extract (solid content concentration 0.7%).

Comparative Production Example 3 Preparation of sunflower root extract The sunflower root was dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4 ° C., and then 60 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 95 g of a light yellow transparent sunflower root extract (solid content concentration 0.3%).

Comparative Production Example 4 Preparation of Sunflower Leaf Extract Sunflower leaves were dried, 60 g of purified water was added to 4 g of the dried product and immersed at 4 ° C., and then 60 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 97 g of a brown transparent sunflower leaf extract (solid content concentration 0.5%).

Formulation Example 1 Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[Component B] Part Extract solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [C component]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the extract solution in Production Example 2 was used instead of the extract solution in Production Example 1 contained in Component B of Formulation Example 1. .

Formulation Example 3 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Extract solution of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts [C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 4 Emulsion In the component B of Formulation Example 3, the emulsion was prepared in the same manner as Formulation Example 10 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Formulation Example 5 Emulsion In the same manner as in Formulation Example 10, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 6 Emulsion In the same manner as in Formulation Example 10, except that 3.0 parts of nicotinamide is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the B component of Formulation Example 3. An emulsion was obtained.

Formulation Example 7 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[Component B] Part Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 8 Lotion [Component] part Extract solution of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Formulation Example 9 Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Extract solution of Production Example 2 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Formulation Example 10 Essence Essence was obtained in the same manner as in Formulation Example 8, except that 5.0 parts of the extract solution of Production Example 2 was used instead of the extract solution of Production Example 1 among the components of Formulation Example 8.

Example 11 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Part Extract solution of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 12. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[Component B] Part Extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 13 Hair growth cosmetics [ingredients] Part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract solution of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 14. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract solution of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount of 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo It was.

Example 15. Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Extract solution of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water in an amount of 100 parts A component and B component were each heated to 80 ° C. and dissolved uniformly, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Formulation Example 16. Beverage [Ingredients] part Extract solution of Production Example 1 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 17. Tablet [Ingredient] part Extract of Production Example 2 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After the above-mentioned parts by weight of each component were mixed, they were pressure-molded to obtain tablets.

Test Example 1 Evaluation of inhibition of melanin synthesis <Experimental method>
B16-F10 mouse melanoma cells were prepared to 1 × 10 ⁵ cells / mL in 10% FBS-containing Eagle's minimum essential medium (MEM), and 1 mL was seeded in a 6 mmφ petri dish. Incubated at 0 ° C. After 24 hours, the culture solution containing the extract of Production Example 1 and 1 mM theophylline was additionally added, and further cultured for 72 hours. Here, the extract of Production Example 1 was prepared such that the final concentration was 1.0% as a solution with respect to the total amount of the culture broth. In addition, as a control, a test group in which a culture solution containing 50% 1,3-butylene glycol was additionally added instead of the extract of Production Example 1 was set. In addition, as a comparative control of the effect, a test group was added to which a culture solution containing each extract of Comparative Production Examples 1 to 4 was added so that the final concentration was 1% instead of the extract of Production Example 1. . After completion of the culture, the supernatant was removed, and the cells were collected using trypsin. A melanin solution (10% dimethyl sulfoxide-containing 1N sodium hydroxide aqueous solution) was added to the collected cells, followed by boiling for 10 minutes to obtain a cell lysate. The value obtained by measuring the absorbance at 490 nm of the cell lysate using an absorbance plate reader (Model 680, manufactured by Bio-Rad) was taken as the amount of melanin. After measuring the amount of melanin, the amount of protein in the cell lysate was measured, and the amount of melanin synthesis per amount of protein was calculated.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, the extract of Production Example 1 according to the present invention shows a remarkable melanin production inhibitory effect, which is compared with other parts of the sunflower (flowers, stems, roots, leaves). It was excellent.

Test Example 2 SOD-like action evaluation test <Experimental method>
0.2 M Tris-HCl buffer solution 50 μL, 1 mM ethylenediaminetetraacetic acid (EDTA) disodium solution 20 μL, 0.75 mM nitroblue tetrazolium chloride (NBT) solution 10 μL, 1 mM xanthine solution 20 μL, 0.06 U / mL xanthine oxidase solution 50 μL A sample solution was prepared by mixing 50 μL of the extract of Example 1. Here, two types of sample solutions were prepared with final concentrations of 0.5% and 1.0%, respectively, as the extract solution of Production Example 1 based on the total amount. This sample solution was incubated at 37 ° C. for 5 minutes to generate superoxide. Then, the amount of formazan produced by reduction of NBT by superoxide was measured for absorbance at 560 nm using an absorbance plate reader (Model 680, manufactured by Bio-Rad). Further, as a control, instead of the extract of Production Example 1, the same operation as described above was performed using a 50% 1,3-butylene glycol solution, and the absorbance at the time of adding each sample to the absorbance obtained here. Was determined as a superoxide residual rate (%). Further, instead of the extract of Production Example 1 as a comparative control, the extracts of Comparative Production Examples 1 to 4 (prepared so that the final concentrations as solutions are 0.5% and 1.0%, respectively) are included. The same operation as described above was performed using the sample solution, and the effects were compared. Moreover, in order to confirm whether the test system is functioning normally, the same test was also performed when 8.75 U / mL of superoxide dismutase (SOD) was added as a positive control instead of the sample solution. .

The results of Test Example 2 are shown in Table 2.
[Table 2]

  As shown in Table 2, it was revealed that the extract according to the present invention showed significantly superior SOD-like activity compared to other parts (flowers, stems, roots, leaves).

Test Example 3 Evaluation test for suppressing oxidative damage in fibroblasts <Experimental method>
Prepare human dermis-derived fibroblasts (NB1RGB) at 2 × 10 ⁵ cells / mL in 0.5% NCS-containing Eagle's minimum essential medium, inoculate 100 μL in a 96-well microplate, 5% carbon dioxide, saturated Culturing was performed at 37 ° C. under water vapor. After 24 hours, a culture solution containing the extract of Production Example 1 was added and cultured. Here, two types of sample solutions were prepared with final concentrations of 0.5% and 1.0%, respectively, as the extract solution of Production Example 1 based on the total amount. In addition, a test group to which a culture solution containing 50% 1,3-butylene glycol was added so that the final concentration was 1.0% was set as a control. Further, instead of the extract of Production Example 1 as a comparative control, the extracts of Comparative Production Examples 1 to 4 (prepared so that the final concentrations as solutions are 0.5% and 1.0%, respectively) are included. A test group (comparison group) to which the sample solution was additionally added was set. After 48 hours, the supernatant of the test group was removed, 10 μM 2 ′, 7′-dichlorodihydrofluorescein diacetate (DCFH-2DA) was taken into the cells, and then irradiated with UV. After UV irradiation, the supernatant was removed and 1% Triron X-100 was added to obtain a cell lysate. The fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) of the cell lysate was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was taken as the amount of oxidative damage. After the measurement, the amount of protein in the cell lysate was measured, and the amount of oxidative damage per amount of protein was calculated.

[Table 3]

As shown in Table 3, the extract of Production Example 1 according to the present invention is significantly superior in suppressing oxidative damage in fibroblasts compared to extracts of other parts (flowers, stems, roots, leaves). Showed the effect.

With the above effects, the cosmetic and cosmetic oral composition containing the extract according to the present invention prevents and improves pigmentation of skin spots, buckwheat, liver spots, etc., and also contains ultraviolet rays, air pollutants, The skin can be protected from oxidative damage caused by allergens such as pollen, and the effects of improving rough skin, aging of the skin, etc. due to those causes can be exhibited.

Claims (4)

A whitening agent containing, as an active ingredient, an extract of a sprout of Asteraceae sunflower (Helianthus annuus). An anti-aging agent comprising, as an active ingredient, an extract of a bud of the sunflower (Helianthus annuus) of the genus Asteraceae (Helianthus). A rough skin ameliorating agent comprising an extract of a sprout of a sunflower (Helianthus annuus) of Asteraceae (Helianthus) as an active ingredient. Cosmetics containing an extract of shoots of Asteraceae sunflower (Helianthus annuus) as an active ingredient.