JP2015199671A - Composition for moisture retention - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a functional material which is derived from natural products and has an excellent biosafety, as well as which is contained in an external preparation for skin, such as cosmetics (quasi drugs are also included) or the like, or oral compositions for cosmetics.SOLUTION: The invention relates to a filaggrin production promoter containing as an active ingredient one or more kinds selected from squeezed juice and/or extract of Solanum Melongena; Egg plant c.v. 'Mizu-nasu', and a composition for moisture retention containing the filaggrin production promoter.

Description

  The present invention relates to a filaggrin production promoter excellent in biological safety and a moisturizing composition containing the filaggrin production promoter.

  The skin is mainly composed of three layers of the epidermis, dermis and subcutaneous tissue, and the amount of water in the stratum corneum of the epidermis is deeply involved in keeping the skin healthy. However, the water content of the stratum corneum may be reduced by external environmental factors (ultraviolet rays, chemical substances, house dust, pollen, drying, etc.) or genetic factors. If an appropriate amount of water is not retained in the stratum corneum due to these factors, the skin's suppleness, smoothness, flexibility, freshness, and transparency are lost. Conventionally, as a component that keeps skin healthy and enhances suppleness, smoothness, flexibility, freshness, or transparency of the skin, for example, natural moisturizing factor (NMF) sugars, amino acids, organic acids, pyrrolidone carvone Acids, collagen, mucopolysaccharides such as hyaluronic acid, elastin, placental extract, glycerin, 1,3-butylene glycol, etc. have been used, but these effects are temporary, and biosafety The surface was not enough.

  Therefore, as a result of intensive studies in view of the above-described conventional problems, the present inventors have found that the eggplant genus Solanum (Solanum) has a water eggplant (Solanum Melongena; Egg plant cv. 'Mizu-nassu). It was found that the juice or extract of ') had a filaggrin production promoting action involved in maintaining the water content of the stratum corneum of the epidermis, and the present invention was completed. Conventionally, squeezed juice or extract of water eggplant has an effect of promoting collagen production of epidermal cells and dermal fibroblasts, an effect of promoting collagen gel contraction and integrin production, and further, an inhibitory effect on tyrosinase activity and an inhibition of melanin production Although having the action is disclosed in Patent Documents 1 to 3, nothing has been reported that the squeezed squeezed juice or extract has a filaggrin production promoting action.

JP 2008-069074 JP 2011-157283 A JP 2011-020951 A

  TECHNICAL FIELD The present invention relates to a filaggrin production promoter containing one or more selected from squeezed juice and / or extract of water eggplant as an active ingredient, and a moisturizing composition containing the agent.

  The filaggrin production promoter of the present invention is based on the excellent filaggrin production promoting action of the squeezed water eggplant extract and / or extract contained as an active ingredient, prevents a decrease in the water content of the stratum corneum of the epidermis, and is supple to the skin. Smoothness, flexibility and freshness can be given and the transparency of the skin can be enhanced. It can also improve diseases associated with a decrease in the amount of water in the stratum corneum, such as atopic dermatitis, ichthyosis, and senile xeroderma.

Hereinafter, the present invention will be described in detail. In the following description, the term cosmetic is used in a broad sense that includes quasi-drugs in addition to so-called cosmetics.
In the present invention, water eggplant is a plant species belonging to the genus Solanum (Solanum Melongena; Egg plant cv 'Mizu-nasu'). For example, seven types of conventional strains, Minamata soft, Izumi Minako, Quanzhou Kinkawa Minako, etc. can be used, but the present invention is not limited to them.

  The squeezed juice of water eggplant can be prepared by pressing the fruit as it is or by crushing the fruit and then pressing the crushed material. Water eggplant extract is dried powder obtained by drying and pulverizing the water eggplant fruit, or by freeze-drying the water eggplant fruit or, in some cases, the juice. Is extracted with a solvent.

  In the following, the method for preparing an extract will be described by taking a method for extracting from a fruit as an example, but the preparation of an extract from a dried powder of squeezed liquid can also be performed according to this method. Preparation of extract from water eggplant fruit is made by bringing the fruit into contact with an extraction solvent by appropriate means such as dipping method or countercurrent extraction method after pre-washing it with water, drying treatment etc. if necessary. Can be done by. A supercritical extraction method may also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; carbonization such as n-hexane, toluene and chloroform Examples thereof include hydrogen-based solvents, which are used alone or in combination of two or more.

  In the present invention, among these solvents, those having a high polarity are particularly preferably used. Furthermore, it is preferable to use one kind of single solvent or two or more kinds of mixed solvents selected from water, lower alcohols and polyhydric alcohols from the viewpoint that they can be widely applied to cosmetics. Is most preferably used alone.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1 if the mixed solvent is water and glycerin. In the case of a mixed solvent of water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 20: 1.

  In the preparation of the extract of the present invention, the pH of the extract is preferably maintained in the range of 5 to 9. In this sense, if necessary, the above extraction solvent may contain sodium hydroxide, sodium carbonate, potassium hydroxide. Alternatively, an alkaline adjusting agent such as arginine, an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, or the like may be blended to adjust to a desired pH.

  Extraction conditions such as extraction temperature and time vary depending on the type of solvent used. For example, in the case of the immersion method, the extraction temperature is generally 0 to 90 ° C., and the extraction time is about 1 to 90 hours. is there.

  When the above extraction process is completed, the liquid phase is separated by applying solid-liquid separation means such as filtration and centrifugation, and the pH is generally adjusted to 4 to 8 which is a preferable range as a cosmetic ingredient. In addition, it is blended in cosmetics as it is or after adjusting to an appropriate concentration by dilution or vacuum concentration. If necessary, this liquid phase may be powdered according to conventional methods such as spray-drying and freeze-drying and blended into cosmetics. In some cases, the extraction stock solution before applying solid-liquid separation means It can also be blended into the material. Moreover, you may give this to the squeezed juice of water eggplant in said powdering process.

  In addition, the squeezed juice or extract of water eggplant of the present invention may be hydrolyzed to the fruit, juice or extract of water eggplant. Thereby, the effect that the operativity at the time of extraction and the storage stability of the obtained extract are improved is expected. As conditions for the hydrolysis treatment, conventional methods such as acid hydrolysis, alkali hydrolysis, enzyme hydrolysis and the like can be used.

  In the case of subjecting the fruit of water eggplant, or the juice or extract thereof to enzymatic hydrolysis, the enzymes include proteolytic enzymes such as actinase and papain, starch-degrading enzymes such as glucoamylase and α-amylase, cellulase and hemicellulase. , One or more selected from fibrinolytic enzymes such as pectinase and lipolytic enzymes such as lipase are used, in particular one or more enzymes selected from each of these four enzyme groups Are preferably used in combination.

  In the case of using a fruit or extract powder, the enzyme hydrolysis treatment for the fruit of water eggplant or the juice or extract thereof with those enzymes is preferably pre-crushed and fragmented before using them. If water or a mixed solution of water and lower alcohol or polyhydric alcohol, preferably suspended or dissolved in water and then using fruit juice or extract solution, adjust the concentration of these solutions as they are or as necessary. After performing etc., an enzyme is added to this and it hold | maintains at the optimal temperature vicinity of an enzyme for 1 to 24 hours.

  The amount of enzyme added is 0.01 to 10% by weight, preferably 0.1 to 1.0% in total, based on the solid content, when fruits are used as the object to be treated. Moreover, when using squeezed juice, an extract solution, or an extract powder, it is 0.001-5 weight in total similarly with respect to solid content, Preferably it is the range of 0.01-0.5 weight%.

  When the above enzymatic hydrolysis treatment is completed, the treatment liquid is subjected to a heat treatment to deactivate the enzyme, and the liquid obtained here is collected as it is or by filtration, centrifugation, etc. Further, if necessary, after pH adjustment, concentration adjustment, powdering treatment, etc., it is used as a raw material for cosmetics.

  Examples of the product obtained by blending the squeezed juice and / or extract of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, lipsticks, foundations, liquid foundations, makeup presses, etc. Makeup cosmetics such as powders, hair shampoos, hair rinses, hair treatments, conditioners, hair dyes, hair cosmetics such as hair stylings, cleansing cosmetics such as facial cleansers, body shampoos, soaps, and bath preparations Of course, it is not limited to these. In addition, as cosmetic oral compositions, beverages such as beauty drinks, energy drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (including powdered soups), dairy products, jelly, candy, Foods such as tablet confectionery, gums, etc .; tablets, liquids, granules or jelly-like health foods / beverages can also be incorporated, but the present invention is not limited to this, and it is incorporated into foods and drinks that can be taken orally. be able to.

  The squeezed juice and / or extract of the water eggplant in the cosmetics of the present invention is generally in the range of 0.0001 to 5% by weight, preferably 0.001 to 3% by weight, as a solid content, for example, for basic cosmetics. The makeup cosmetics are generally in the range of 0.0001 to 5% by weight, preferably 0.001 to 2% by weight, and the hair cosmetics are generally 0.0001 to 10% by weight, preferably 0.001 to 0.001%. The range is 5% by weight, and the cleaning cosmetic is generally in the range of 0.0001 to 10% by weight, preferably 0.001 to 5% by weight. Moreover, about the cosmetic oral composition, the range of 0.1 to 15 weight% is preferable as solid content.

  When blending the extract and / or extract of water eggplant according to the present invention into cosmetics, components usually used in cosmetics, for example, oily components, surfactants, moisturizers, thickeners, antiseptic / disinfectants , Powder ingredients, ultraviolet absorbers, physiologically active ingredients (whitening ingredients, anti-aging / skin-beautifying / wrinkle-preventing ingredients, etc.), anti-inflammatory agents, antioxidants, pigments, fragrances, etc. it can. Moreover, a known whitening agent can also be mix | blended as long as the effectiveness and the characteristic of the squeezed juice of the water eggplant of this invention and / or its extract are not impaired.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers and emulsifiers include stevia derivatives such as enzyme-treated stevia, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.) It can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white) extract; polysaccharides such as pectin, locust bean gum and aloe polysaccharide; xanthan gum, Gums such as tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives; Examples include glutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry matter, and the like.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.).

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), 4-HPB (rhodenol, 4- (4-hydroxyphenyl) -4-butanol)), hydroxybenzoic acid And derivatives thereof, vitamin E and derivatives thereof, α-hydroxy acid, and AMP (adenosine monophosphate, adenosine monophosphate). These may be used alone or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, ascorbic acid sugar derivatives such as L-ascorbic acid-5-glucoside, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is Hexanoyl group, o L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid- 2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acylated derivatives thereof, ascorbyl glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid As hydroquinone derivatives, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as tranexamic acid derivatives, tranexamic acid ester ( For example, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, tranexamic acid methylamide) and the like, and resorcinol derivatives include, for example, 4-n- Butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid derivatives such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic acid derivative include nicotinic acid amide and benzyl nicotinate, and examples of the α-hydroxy acid include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

  As a physiologically active ingredient, as a whitening ingredient, for example, placenta extract, Sakuhakuhi extract, yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, white coconut Fermented product, peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract, pearl hydrolyzed product, pearl seed fermented product, royal jelly Fermented product, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. Coral grass extract as an anti-aging component , Rice leaf extract or its hydrolyzate, apricot fruit extract, seaweed extract such as catamen giraffe Extracts of marine flowering plants such as fermented soybean milk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzed product thereof, fermented rice fermented product, black bean extract or its Hydrolyzate, damask rose flower extract, bamboo shoot extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), animal or fish-derived collagen and its derivatives, elastin and its derivatives Glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract , Aloe extract, Honeycomb extract, Loofah extract, Anaaaosa extract, Juase Iro (Zizyphus joazeiro) extract etc.

  Next, although a manufacture example, a test example, and an Example (formulation example of cosmetics) are given and this invention is demonstrated further more concretely, this invention is not limited to them. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of water eggplant juice (1)
2800 g of water eggplant (from Quanzhou, the same below) was made into a paste using a food processor, and the resulting liquid was further filtered to obtain 2010 g of a tan clear water eggplant juice (solid content concentration). 4.10%).

Production Example 2 Preparation of water eggplant juice (2)
2800 g of water eggplant fruit was made into a paste using a food processor, and the resulting liquid was decolorized with activated carbon to obtain 1920 g of a colorless and transparent water eggplant juice (solids concentration 3.85%). ).

Production Example 3 Preparation of water eggplant fruit extract (1)
Purified water 1000 g was mixed with 100 g of dried eggplant fruit, and extracted at 40 ° C. for 4 hours, followed by filtration to obtain 800 g of a yellow clear water eggplant fruit extract (solid content 1.50%). .

Production Example 4 Preparation of water eggplant fruit extract (2)
Purified water and 1200 g of a 7: 3 (weight ratio) mixture of 1,3-butylene glycol were mixed with 100 g of dried eggplant fruit, extracted at 80 ° C. for 2 hours, filtered, and tan-clear water Eggplant fruit extract 893g was obtained (solid content concentration 1.01%).

Production Example 5 Preparation of water eggplant fruit extract (3)
100 g of dried eggplant fruit mixture was mixed with 800 g of a 1: 1 (weight ratio) mixture of purified water and ethanol, extracted at 4 ° C. for 72 hours, filtered, and 635 g of a pale yellow clear water eggplant fruit extract. (Solid content concentration 0.80%) was obtained.

Production Example 6 Preparation of water eggplant fruit extract (4)
100 g of dried eggplant fruit was pulverized with a pulverizer and sieved to obtain 84 g of dried eggplant fruit powder having an average particle size of 20 μm.

Production Example 7 Preparation of water eggplant juice liquid powder 500 g of water eggplant juice liquid obtained in the same manner as in Production Example 1 was freeze-dried and then pulverized to obtain 19.7 g of tan brown water eggplant juice liquid powder.

Example 1. Cream [Component A] Liquid paraffin 5.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
[Component B] Part Juice eggplant juice of Production Example 1 10.0
Glycerin 5.0
Methylparaben 0.1
Collagen 2.0
Purified water Amount that makes the total amount 100 parts [C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the water eggplant juice of Production Example 2 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 1.

Example 3 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Phenoxyethanol 0.03
[Component B] Part Juice eggplant juice of Production Example 1 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts [C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 4 Lotion [Ingredients] part Water eggplant juice of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts.

Example 5 FIG. Lotion [A component] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl ether 0.5
Butylparaben 0.1
[Component B] Part Water eggplant juice of Production Example 2 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Total amount of 100 parts [Component C] Part Fragrance Appropriate amount Each component A and component B is heated to 80 ° C. or higher, and then added to component A and stirred. ) For 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 6 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant juice extract of Production Example 3 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 7 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant fruit extract of Production Example 4 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 8 FIG. Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant fruit extract of Production Example 5 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 9 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[Component B] Part Juice eggplant juice of Production Example 1 10.0
L-ascorbic acid-2-glucoside 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total [Component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 10 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 11 Emulsion In the component B of Example 9, the emulsion was prepared in the same manner as in Example 9 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 12 Emulsion In the same manner as in Example 9, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used in the component B of Example 9 instead of 2.0 parts of nicotinamide. An emulsion was obtained.

Example 13 Emulsion Emulsion in the same manner as in Example 9 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide are used in the component B of Example 9 instead of 2.0 parts of tranexamic acid. Got.

Example 14 Emulsion In the same manner as in Example 9, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used in the component B of Example 9 instead of 5.0 parts of potassium hydroxide. An emulsion was obtained.

Example 15. Emulsion [Component A] Part Liquid paraffin 6.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[Component B] Part Extract solution of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 16 Lotion [component] part water eggplant juice of production example 2 3.0
L-ascorbic acid-2-glucoside 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Sodium alginate 0.1
Potassium hydroxide Appropriate amount Perfume Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 17. Pressed powder [A component] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
6-Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Mizu eggplant fruit dried product powder of Production Example 6 0.1
[Component B] Part Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Fragrance Appropriate Amounts The above A component and B component were mixed and stirred, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 18 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[Component B] Part Juice eggplant juice of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 19. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[Component B] Part Water eggplant juice of Production Example 2 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [Component C] Part Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D] part fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 20. Hair artic [component A] part ethanol 60.0
l-Menthol 0.5
Fragrance 0.1
Methylparaben 0.1
[Component B] Part Glycerin 2.0
1,3-butylene glycol 2.0
Juice eggplant fruit extract of Production Example 5 10.0
Purified water in an amount of 100 parts The above A component and B component were each dissolved at room temperature, then the B component was added to the A component with stirring and dissolved to obtain a hair-tonic.

Example 21. Hair treatment [ingredient] part stearyltrimethylammonium chloride 6.0
Polyvinylpyrrolidone 4.0
Glycerin 1.0
Ethylparaben 0.1
Water eggplant fruit extract of Production Example 3 5.0
Purified water in a total amount of 100 parts The above ingredients were heated to 80 ° C and then mixed and stirred to obtain a hair treatment.
This product was also suitable as a hair pack.

Example 22. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[Component B] Part Citric acid 0.1
Mizu eggplant fruit extract of Production Example 4 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a hair shampoo It was.

Example 23. Hair rinse [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Juice eggplant juice of Production Example 1 5.0
1,3-butylene glycol 5.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain hair rinse It was.

Example 23. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Juice eggplant juice of Production Example 2 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 24. Soap [Component A] Part Hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[Component B] Part Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Mizu eggplant juice liquid powder of Production Example 7 0.5
Purified water Amount that makes the total amount 100 parts [Component C] parts Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Formulation Example 25. Beauty drink Production example 1 water eggplant juice 10.0
Collagen 8.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Antioxidant (Vitamin C) 0.01
Amount of purified water totaling 100 parts

Formulation Example 26. Tablets Water eggplant fruit extract 20.0
Vitamin C 20.0
Fatty acid ester 10.0
Calcium lactate 20.0
Lactose 30.0
After the above-mentioned parts by weight of each component were mixed, they were pressure-molded to obtain tablets.

Test Example 1 Evaluation test for promotion of filaggrin production Human epidermal cells PHK16-0b were seeded in a 6-well microplate at a concentration of 2 × 10 ⁵ cells / well. As the medium, MCDB153 (manufactured by SIGMA) to which Epidercel HKGS (manufactured by Kurabo Industries) was added as a growth promoter was used. After pre-culturing at 37 ° C. for 3 days, the sample solution was replaced with a medium containing 1.0% concentration (as a solution concentration), and the cells were further cultured at 37 ° C. for 4 days. As a comparative control, calcium chloride having an effect of promoting epidermal cell differentiation was also replaced with a medium containing 1.8 mM, and the same operation was performed. Next, the medium was removed, and after washing with PBS (−), trypsin-EDTA 0.5 mL / well was added, treated for 5 minutes, and the cells were detached and collected. The obtained cell recovery solution was centrifuged (1200 rpm × 10 minutes, 4 ° C.), and the supernatant was removed. PBS (-) was added and suspended, and centrifuged under the same conditions to obtain a pellet. 0.25 M Tris-HCl (pH 7.8: 9 M urea, 0.1% (v / v) containing Triton X-100) is added to the resulting pellet, suspended, heated at 100 ° C. for 5 minutes, and further subjected to ultrasonic waves. For 5 minutes to lyse the cells. This solution was centrifuged (10000 rpm × 10 minutes), and the collected supernatant was used as a cell lysate. 5 μL of each collected cell lysate was taken, 200 μL of 5-fold diluted Protein Assay Dye Reagent Concentrate (manufactured by Bio-Rad) was added and allowed to stand at room temperature for 5 minutes, and the absorbance at 570 nm was measured. A solution in which BSA was prepared at an arbitrary concentration in advance was measured by the same operation, and the protein concentration of each cell lysate was calculated from the prepared calibration curve. All cell lysates were diluted and prepared so that the protein concentration was 0.8 mg / mL, and used as samples for the dot blot test. The amount of filaggrin synthesis was measured by an enzyme antibody method using a dot blot method. Specifically, the cell lysate prepared above at 0.8 mg / mL was dot-blotted on a nitrocellulose membrane, treated with anti-filaggrin antibody and HRP-labeled IgG antibody, and then EzWestBlue (manufactured by Atto Corporation). Color. The colored membrane was taken in as a digital image, digitized by ImageJ, and the filaggrin synthesis rate in the sample addition group when the filaggrin expression level in the control group was 100% was calculated as a relative value.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract according to the present invention has an excellent filaggrin production promoting effect.

Test Example 2 Measuring method of stratum corneum water content A test part (1 cm × 1 cm) is provided on the inner side of the left forearm of the test subject, and the stratum corneum water content of each test part is measured using Skicon-200, and the average is the initial value of each test part. Value. After measuring each initial value, set two test plots and control plots, apply lotion containing 5.0% of the extract of the production example of the present invention (apply to test plot), and purify instead of sample Application of lotion containing water (application to the control area) was started (twice a day). After 6 days, the stratum corneum water content of each test part was measured in the same manner.

The results of Test Example 2 are shown in the table.
[Table 2]

As shown in Table 2, it was confirmed that the extract according to the present invention significantly improves the retention of the skin stratum corneum moisture content.

Claims (2)

The filaggrin production promoter characterized by mix | blending 1 type (s) or 2 or more types chosen from the squeezed liquid and / or extract of water eggplant (Solanum Melongena; Egg plant cv.'Mizu-nasu '). A moisturizing composition containing the filaggrin production promoter according to claim 1.