JP2016124827A - Cosmetics - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide functional materials consisting of a naturally occurring component that is excellent in biosafety and effectiveness, the functional materials having health improving and antiaging effect of cells.SOLUTION: The invention provides an inhibitor of protein glycosylation containing extract of Prunus persica belonging to Rosaceae as an active ingredient, a proteasome activator of fibroblasts and epidermal cells, and a heat shock protein expression enhancer, which are functional materials preventing and improving cell aging and damage, and repairing the cell damage by ultraviolet light or other external stimulus factors.SELECTED DRAWING: None

Description

  An object of the present invention is to provide a functional material obtained from a plant of the Rosaceae family and having excellent cell health and anti-aging effects.

  In recent years, research on damage caused by cellular aging and external factors (for example, ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.) has been conducted, and various cellular aging phenomena have been conducted. In addition, mechanisms related to cell damage and repair have been elucidated.

  For example, the glycation reaction of intracellular protein is known as a factor of cell aging, and the accumulation of the final product of protein glycation reaction product called advanced glycation end product (AGE), which is caused by the reaction, is known. It attracts attention. When this protein saccharification reaction occurs in the cell, the function of the protein (collagen, elastin, etc.) in the cell is impaired, thereby deteriorating the cell.

  It is also known that a decrease in the function of proteasomes present in cells affects aging. This proteasome is distributed in both cytoplasm and nucleus in eukaryotic cells, and is a huge enzyme complex that degrades proteins in cells. For example, the proteasome breaks down proteins that have been degraded by UV rays or active oxygen (proteins that have been cross-linked, glycated or carbonylated) and prevents the degradation proteins from accumulating in the cells, contributing to the maintenance and improvement of cell functions. It is known to do.

  In addition, a protein (heat shock protein: HSP) that repairs proteins in cells damaged by stress such as ultraviolet rays and physical stimulation has attracted attention as a factor for improving aging and damage of cells.

  In light of the above, the present inventors have conducted extensive research to find new functional materials derived from natural products. As a result, the peach extract belonging to the family Rosaceae has an excellent protein glycation-inhibiting effect and proteasome activity. It has the effect of enhancing the expression of heat shock protein and heat shock protein, and by these interactions, it has excellent cell health and anti-aging effects by blending the extract, for example, excellent cosmetics I found out that it would be possible.

  Conventionally, it has been known from Patent Documents 1 to 5 that a peach extract belonging to the family Rosaceae is used as an anti-aging agent, but the peach extract is used as a protein glycation inhibitor, a proteasome activity enhancer, and It has not been known to use it as a heat shock protein expression enhancer.

JP 07-309770 A Japanese Patent Laid-Open No. 10-330222 JP 2001-199867 JP 2008-247783 A JP 2009-256244 A

The present invention is a protein saccharification inhibitor containing, as an active ingredient, an extract of peach (Prunus persica) belonging to Rosaceae.
Moreover, this invention is a proteasome activator which contains the extract of the peach (Prunus persica) which belongs to the Rosaceae (Rosaceae) as an active ingredient.
The present invention is also a heat shock protein expression enhancer containing, as an active ingredient, an extract of peach (Prunus persica) belonging to Rosaceae.
The present invention is also a cosmetic containing a protein saccharification inhibitor, a proteasome activator or a heat shock protein expression enhancer containing an extract of peach (Prunus persica) as an active ingredient.
In the present specification, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

  The present invention is a protein saccharification inhibitor comprising a peach extract belonging to the family Rosaceae as an active ingredient, and according to the present invention, the accumulation of degraded proteins in the cells is suppressed to prevent aging and damage of the cells, Functional materials that can be improved can be provided. The present invention is also a proteasome activator comprising a peach extract belonging to the family Rosaceae as an active ingredient. According to the present invention, the proteasome promotes the degradation of degraded proteins and prevents cellular aging and damage. Can provide functional materials that improve. The present invention also relates to a heat shock protein expression enhancer comprising a peach extract belonging to the family Rosaceae as an active ingredient. According to the present invention, the present invention repairs cell damage caused by ultraviolet rays and other external stimulating factors. Functional materials can be provided. Furthermore, since the extract of the present invention further has an active oxygen scavenging action, it can provide an agent that has an excellent effect of preventing and improving cell aging and damage.

Hereinafter, preferred embodiments of the present invention will be described in detail.
The extraction material used in the present invention is peach (Prunus persica) belonging to Rosaceae. The species of peach according to the present invention is not particularly limited, and any species belonging to peach may be used. For example, white peach, white peach, and golden peach peaches are listed.

  For the preparation of the extract, first, the peach part (for example, flowers, leaves, flowers and leaves, or whole grass) is washed with water in advance if necessary to remove foreign substances, and then left as it is or after drying. Depending on the size, it is chopped or pulverized and contacted with an extraction solvent for extraction. The extraction can be performed by contacting with an extraction solvent according to a conventional method such as an immersion method, but a supercritical extraction method or a steam distillation method can also be used.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol, propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin; ethyl acetate, butyl acetate, methyl propionate, etc. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon solvents such as n-hexane, toluene and chloroform, and the like. These may be used alone or in combination of two or more. Used.

  Among these extraction solvents, the effectiveness of the obtained extract, further, from the viewpoint of biosafety, and also from the viewpoint that it can be widely applied to various products. Hydrophilic solvents such as alcohols or polyhydric alcohols are preferred. Preferred examples of the case where this hydrophilic solvent is used include, for example, water, lower alcohols (ethanol, etc.), polyhydric alcohols (1,3-butylene glycol, glycerin, etc.) used alone, or water and lower alcohols. Use of a mixed solvent with alcohol (such as ethanol) or a mixed solvent of water and a polyhydric alcohol (such as 1,3-butylene glycol, glycerin).

  When the mixed solvent is used, for example, if the mixed solvent is water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1: 3 to 20: 1, and the mixed solvent of water and ethanol. If it is, it is preferable to set it as the range of 1: 1 to 20: 1 if it is a mixed solvent of 1: 3 to 25: 1 and water and glycerol.

  Moreover, the weight ratio of the dried part of peach (eg, flowers, leaves, flowers and leaves, or whole grass) and the extraction solvent is preferably in the range of 1: 1 to 1: 150, more preferably 1: It is in the range of 5 to 1: 120.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. The extraction temperature is preferably in the range of 0 ° C. to 90 ° C., more preferably in the range of 20 ° C. to 85 ° C., and the extraction time is preferably 0.5 hours to 7 days, more preferably. It ranges from 1 hour to 3 days.

  In addition, you may perform a hydrolysis process to an extraction site | part as needed prior to the extraction process of this invention, or in parallel with an extraction process. As a result, the storage stability of the extract may be improved and the extract may be used more effectively.

  In the case of subjecting the extract to an enzymatic hydrolysis treatment, the enzymes include proteolytic enzymes such as actinase, papain, chymopapain or pepsin, starch degrading enzymes such as glucoamylase, α-amylase or β-amylase, cellulase, hemicellulase or pectinase. 1 type or 2 types or more selected from any enzyme group of a fibrinolytic enzyme such as lipase and a lipolytic enzyme such as lipase may be used, but 1 type or 2 selected from each of these enzyme groups You may use combining the enzyme of a seed | species or more.

  For example, if the amount of the enzyme is a peach flower, leaf, flower and leaf, or whole plant, the total amount is preferably in the range of 0.01 to 50% by weight based on the solid content. Preferably it is 0.1 to 10 weight% of range.

  In general, the extract prepared as described above may be adjusted to a pH of 3 to 9 and used as it is as a cosmetic compounding agent as it is or as a desired concentration by vacuum concentration or the like. You may do it. In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the extract prepared as described above may be stored after refrigeration for a certain period of time in order to enhance storage stability and the like, and then the supernatant may be used.

  When the extract according to the present invention is used, for example, in cosmetics (including quasi-drugs), the dosage forms include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, and makeup. Examples include press powder, blusher, white powder, face wash, body shampoo, hair shampoo, soap and the like. Moreover, although a hair growth agent and also a bath agent etc. are mentioned, of course, it is not limited to these. The extract according to the present invention can also be blended in an oral composition, for example, beverages such as beauty drinks, nutrition drinks, sports drinks, near water, vitamin drinks, mineral drinks, alcoholic drinks; various soups (Including powdered soup), dairy products, jelly, candy, tablet confectionery, gum and other foods; tablets, liquids, granules or jelly-like health foods / beverages, etc. It is not limited and can be blended in foods and drinks that can be taken orally.

  The amount of the extract of the present invention in cosmetics is generally 0.002 to 1.0% by weight (solid content% by weight, the same applies hereinafter), preferably 0 as the solid content of the extract in the case of basic cosmetics. In the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. The case is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 3.0% by weight. Moreover, the compounding quantity of the extract of this invention in an oral composition has the preferable range of 0.1 to 15 weight% as solid content of an extract.

  When the extract according to the present invention is blended in cosmetics, in addition to the extract which is an essential component, components usually used in cosmetics such as oily components, surfactants (synthetic systems, natural products), moisturizing agents An agent, a thickener, an antiseptic / bactericidal agent, a powder component, an ultraviolet absorber, an antioxidant, a pigment, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the extract are not impaired, there may be no combination of other physiologically active ingredients.

  Here, as the oil component, for example, olive oil, bergamot oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, Oils derived from plants such as avocado oil, macadamia nut oil, plant-derived squalane; Oils derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax , Hydrocarbons such as squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; isopropyl myristate, isopropyl palmitate , Butyric oleate , 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended. Moreover, these emulsifiers or emulsification aids may be nanoparticles formed by high-speed stirring treatment, ultrasonic treatment, or the like.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Plant-derived ethanol such as ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermented liquor, sugarcane Alternatively, there is 1,3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, cereal (rice, wheat, corn, millet, etc.) powder, legume (soybean, red bean, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as prillyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxy. Cibenzoic acid and the like, nicotinic acid derivatives include, for example, nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid, and the like. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, party extract or hydrolyzate thereof, pearl hydrolyzate, pearl seed fermented product, Royal jelly fermented product, sake lees extract or ceramide contained therein, sake lees fermented product, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract, etc. It is done. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe Extracts of seaweeds such as seaweed, extracts of marine flowering plants such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermentation extract, germinated rice extract or hydrolyzate thereof, germinated rice Fermented product, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), animal or fish-derived collagen And derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diiso Lopyramine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, red ginseng extract, beetroot extract, loofah extract, anaasa extract , Peach extract, peach extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, paudarco bark extract, dairy lily extract or ferment, hibiscus flower extract or ferment, hagoromogusa Extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red rich extract, purple orchid extract, yam peel or seed coat extract or hydrolysate, safflower flower extract, Casablanca Extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, evening birch extract , Aloe extract, fig flower extract, apple extract, there is bergamot extract or the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of peach extract A dried product of peach flowers of the genus Rosaceae was pulverized. After adding 1000 g of 50% 1,3-butylene glycol to 10 g of this powder, extraction was performed at 40 ° C. The extract was filtered to obtain 830 g (solid content concentration 0.30%) of a brown transparent peach blossom extract solution.

Production Example 2 Preparation of peach extract A dried product of peach flowers of the genus Rosaceae was pulverized. After adding 1000 g of purified water to 10 g of this powder, extraction was performed at 40 ° C. The extract was filtered to obtain 950 g of a brown transparent peach flower extract solution (solid content concentration: 0.36%).

Production Example 3 Preparation of peach extract A dried product of peach leaves of the family Rosaceae was pulverized. After adding 300 g of 50% 1,3-butylene glycol to 10 g of this powder, extraction was performed at 80 ° C. The extract was filtered to obtain 223 g of a brown transparent peach leaf extract solution (solid content: 0.90%).

Production Example 4 Preparation of peach extract A dried product of peach leaves of the family Rosaceae was pulverized. 450 g of purified water was added to 15 g of this powder, followed by extraction at 80 ° C. This extract was filtered to obtain 358 g of a brown transparent peach leaf extract solution (solid content concentration: 1.03%).

Formulation Example 1 Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Extract solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [Component C]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 2 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 3 was used instead of the extract of Production Example 1 contained in the component B of Formulation Example 1.

Formulation Example 4 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the extract of Production Example 4 was used instead of the extract of Production Example 1 contained in Component B of Formulation Example 1.

Formulation Example 5 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 1 1.5
Extract of Production Example 3 1.5
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Formulation Example 6 Emulsion Emulsion in the same manner as in Formulation Example 5 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 7 Emulsion In the component B of Formulation Example 5, the emulsion was prepared in the same manner as Formulation Example 5 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Formulation Example 8 Emulsion In the same manner as in Formulation Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 9 Emulsion In the same manner as in Formulation Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. An emulsion was obtained.

Formulation Example 10 Emulsion [Component A] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
[B component]
Extract of Production Example 2 2.0
Extract of Production Example 4 2.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Arbutin 3.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 11 Lotion [Ingredient] part Extract of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Dipotassium glycyrrhizinate 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Formulation Example 12. Lotion A lotion was obtained in the same manner as in Formulation Example 10, except that 10.0 parts of the extract of Production Example 4 was used instead of the extract of Production Example 2 in the ingredients of Formulation Example 11.

Formulation Example 13 Essence [ingredient] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid 0.1
Extract of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water in an amount of 100 parts Hyaluronic acid was dissolved in purified water, and then the remaining raw materials were sequentially added and dissolved by stirring to obtain a transparent essence.

Example 14 Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Extract of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Formulation Example 15. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Extract of Production Example 2 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Formulation Example 16. Hair growth cosmetics [ingredients] Part Dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Gentian extract 2.0
Extract of Production Example 1 2.0
Extract of Production Example 2 2.0
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20
The amount in which the total amount of purified water is 100 parts The above ingredients were sufficiently stirred and mixed to obtain a hair restorer.

Formulation Example 17. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Purified water Total amount of 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, stirring is continued and cooled to room temperature to obtain a hair shampoo It was.

Example 18 Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[Component B] Part Extract of Production Example 1 2.0
1,3-butylene glycol 5.0
Purified water in an amount of 100 parts A component and B component were each heated to 80 ° C. and dissolved uniformly, then B component was added to A component, and stirring was continued to cool to room temperature to obtain a hair rinse. .

Test Example 1 Evaluation test of protein saccharification inhibitory effect In this test, the AGE generation inhibitory effect, that is, the protein saccharification inhibitory effect, was evaluated using the fluorescence intensity and absorbance of BSA (bovine serum albumin) produced by reacting with glucose as indices. Next, 50 μL of the preparation solution, 50 μL of 40 mg / mL BSA aqueous solution, 50 μL of 2.0 M glucose aqueous solution, and 50 μL of PBS (−) solution containing 0.2% paraben and 5.0% 1,3-butylene glycol Were mixed and stirred to prepare a sample solution. The sample solution was prepared so that the final concentrations of the extract solution of Production Example 1 were 1.0% and 2.0%, respectively. Next, the sample solution is allowed to stand at 50 ° C. for 7 days, and after 7 days, for each sample solution, fluorescence generation (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader (Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)) And absorbance (wavelength: 405 nm: microplate reader (Model 680, manufactured by Bio-Rad)) was measured. Moreover, it replaced with the extract of manufacture example 1, and performed the same operation about the case of the sample non-addition (Control) using 50% 1, 3- butylene glycol, and with respect to the fluorescence measurement value and absorbance measurement value which were obtained here The relative value (%) between the fluorescence measurement value and the absorbance measurement value of each sample solution was determined and used as the protein saccharification rate (%).

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the extract according to Production Example 1 of the present invention has an excellent protein saccharification inhibitory effect in a concentration-dependent manner.

Test Example 2 Evaluation test of fibroblast proteasome activity enhancement effect Human dermis-derived fibroblasts NB1RGB were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium, 37 ° C., After pre-culturing for 1 day under conditions of 5.0% CO ₂ , add the extract (sample solution) of Production Example 1 to the medium so that the concentration (as a solution) is 0.5% and 1.0%. And further cultured for 3 days under the same conditions. Thereafter, the cell culture supernatant of each test section was removed, and 100 μL of cell lysate (Tris-HCl buffer (pH 8.0) containing 1% Tween 20: 12 mM trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid) was added. A crude enzyme solution was obtained by disrupting the cells. 50 μL of the crude enzyme solution was divided into a separate plate, and 10 μL of a proteasome activity inhibitory solution (a cell lysate containing 2 mM epigallocatechin gallate) was added thereto, which was used for non-proteasome activity measurement. In addition, 10 μL of cell lysate was added to the remaining crude enzyme solution to adjust the volume, and this was used for measuring the total substrate resolution. To each plate, 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added and reacted at 37 ° C. for 1 hour. After completion of the reaction, fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as the proteasome activity. In the case of adding no sample (Control) in which 50% 1,3-butylene glycol was added instead of the sample solution of Production Example 1, the same operation as described above was performed, and each sample was added to the proteasome activity obtained here. The relative value of the proteasome activity was determined and used as the proteasome activity rate (%).

[Table 2]

  As shown in Table 2, it was confirmed that the extract of Production Example 1 according to the present invention enhances the proteasome activity of fibroblasts in a concentration-dependent manner.

Test Example 3 Evaluation test of epidermal cell proteasome activity enhancement effect Normal human skin-derived epidermal cells (NHEK) were seeded at 5 × 10 ³ cells / well in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo Industries, Ltd.) After pre-culturing for 1 day under the condition of 0% CO ₂ , the sample solution of Production Example 1 was added to the medium so as to have a concentration (as a solution) of 0.5% and 1.0%. Cultured for 3 days. Thereafter, the cell culture supernatant of each test section was removed, and 100 μL of cell lysate (Tris-HCl buffer (pH 8.0) containing 1% Tween 20: 12 mM trishydroxymethylaminomethane, 5 mM EDTA, 150 mM NaCl, hydrochloric acid) was added. A crude enzyme solution was obtained by disrupting the cells. 50 μL of the crude enzyme solution was placed in a separate plate, and 10 μL of a proteasome activity inhibitory solution (a cell lysate containing 2 mM epigallocatechin gallate) was added thereto, which was used for non-proteasome activity measurement. In addition, 10 μL of cell lysate was added to the remaining crude enzyme solution to adjust the volume, and this was used for measuring the total substrate resolution. To each plate, 10 μL of a buffer containing 65 μM Suc-Leu-Leu-Val-Tyr-AMC as a substrate was added and reacted at 37 ° C. for 1 hour. After completion of the reaction, fluorescence intensity (excitation wavelength: 355 nm, fluorescence wavelength: 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The value obtained by subtracting the non-proteasome activity measurement result from the obtained total substrate resolution measurement result was defined as the proteasome activity. In the case of adding no sample (Control) in which 50% 1,3-butylene glycol was added instead of the sample solution of Production Example 1, the same operation as described above was performed, and each sample was added to the proteasome activity obtained here. The relative value of the proteasome activity was determined and used as the proteasome activity rate (%).

[Table 3]

  As shown in Table 3, it was confirmed that the extract of Production Example 1 according to the present invention enhances the proteasome activity of epidermal cells in a concentration-dependent manner.

Test Example 4 Evaluation test of effect of enhancing expression of heat shock protein (HSP) Human dermis-derived fibroblast NB1RGB is prepared to 6 × 10 ⁵ cells / mL in 0.5% NCS-containing Eagle's minimum essential medium, and 1 mL is seeded in a φ6 cm dish Then, the cells were cultured at 37 ° C. under 5% CO ₂ and saturated steam. After culturing for 24 hours, the culture solution containing the extract of Production Example 3 (with the extract of Production Example 3 added to a final concentration of 1.0% as a solution with respect to the total amount of the culture solution) Added and cultured. As a comparative control, instead of the extract of Production Example 2, a culture solution containing only 50% 1,3-butylene glycol (the final concentration of 50% 1,3-butylene glycol with respect to the total amount of the culture solution was set to 1%). A test group (control group) to which (adjusted) was added was set. After culturing for 24 hours, the cells in each test group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, stirred and mixed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes with a centrifuge (TOMY / MX-160). Thereafter, 400 μL of the aqueous layer alone was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, stirred and mixed, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to total RNA, stirred and washed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is expressed in a certain amount in common in many tissues and cells, and is always expressed and essential for cell maintenance and proliferation) Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of each gene in each test section was compared when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of each gene in the other test plots when the expression level of each gene in the control plot was set to 100 was determined.

[Table 4]

[Table 5]

As shown in Tables 4 and 5, it was confirmed that the extract of Production Example 3 according to the present invention enhances gene expression of HSP32 and HSP72.

Test Example 5 DPPH radical scavenging effect evaluation test First, 2.4 parts of DPPH (1,1-diphenyl-2-picrylhydrazyl) was dissolved in 20 parts of ethanol, and 20 parts of purified water was added thereto to prepare a DPPH solution. . To 24 parts of this DPPH solution, 19.2 parts of 18 v / v% ethanol solution and 4.8 parts of 2M acetic acid-sodium acetate buffer (pH 5.5) were added to prepare a DPPH-added solution. In addition, in order to subtract the effect of the color of the extract itself on the test, a 50 v / v% ethanol solution was used instead of the DPPH solution, and a solution obtained by mixing an 18 v / v% ethanol solution and a 2M acetic acid-sodium acetate buffer solution was used. A control solution was used. Next, the extract solution of Production Example 1 prepared as described above was diluted with purified water to prepare a sample solution. Here, the sample solution was prepared such that the final concentration (concentration as a solution) of the extract solution of Production Example 1 was 0.5%, 1.0%, and 2.0%, respectively, with respect to the total amount 3 The seed concentration was used. The sample solution and DPPH added solution or control solution were mixed at a ratio of 1: 3, allowed to stand at room temperature for 10 minutes, and the absorbance at 550 nm when each test solution was mixed with DPPH added solution. The difference in absorbance at 550 nm when this was mixed with the control solution was measured to confirm the residual amount of DPPH radicals. At the same time, instead of the extract solution of Production Example 1 as a control, the same operation as described above was performed using a 50% 1,3-butylene glycol aqueous solution, and each sample was added to the DPPH radical residual rate obtained here. The relative value of the DPPH radical residual rate was determined. In addition, in order to confirm whether the test system is functioning normally, the same test was performed when water-soluble vitamin E (final concentration 10 μM) was added as a positive control instead of the sample solution.

The results of Test Example 5 are shown in Table 6.
[Table 6]

  As shown in Table 6, it was shown that the extract according to Production Example 1 of the present invention has an excellent DPPH radical scavenging action depending on the concentration.

Claims (4)

  Protein glycation inhibitor containing, as an active ingredient, an extract of peach (Prunus persica) belonging to Rosaceae.   A proteasome activator containing an extract of peach (Prunus persica) belonging to Rosaceae as an active ingredient.   A heat shock protein expression enhancer containing an extract of peach (Prunus persica) belonging to Rosaceae as an active ingredient. Cosmetics containing the agent as described in any one of Claims 1-3.