JP2019194174A - Skin external preparation - Google Patents

Abstract

To provide a skin external preparation that is excellent in efficacy and quality stability as well as biological safety and has a body hair growth inhibitory effect.SOLUTION: The present invention provides a skin external preparation for inhibiting body hair growth that contains a fermentation product of a plant of Malvaceae, Hibiscus as an active ingredient. Such a skin external preparation is excellent in biological safety and has a body hair growth inhibitory effect. Microorganisms used for fermentation include lactic acid bacteria, yeast, koji mold, or Bacillus subtilis.SELECTED DRAWING: None

Description

  The present invention relates to a skin external preparation excellent in biological safety and having an excellent effect of suppressing hair growth.

  Conventionally, methods (hair removal cream, tape stripping) to remove body hair (hands, feet, heels, torso, face, etc.) have been proposed for aesthetic reasons, but skin irritation and rough skin after body hair removal Problems such as unhealthy skin occurred.

  In view of the problems of the prior art, the present inventors have intensively studied to find a new active ingredient derived from a natural product from the viewpoint of biological safety. As a result, the present inventors have newly found that fermented plants of the genus Fuyo have an excellent hair growth inhibitory effect.

Conventionally, it has been known from Patent Documents 1 and 2 that use extracts of plants belonging to Fuso or fermented products of these plants in cosmetics. It was not done.
JP 57-99517 JP 2006-347925

  The present invention is a skin external preparation for suppressing hair growth, comprising a fermented plant of Fuso spp. As an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the skin external preparation which is excellent in biological safety and has the outstanding hair growth inhibitory effect can be provided.

Hereinafter, preferred embodiments of the present invention will be described in detail.
Examples of the plants of the family Malvaceae Hibiscus used in the present invention include, for example, Roselle (Hibiscus sabdariffa L.), Mucuge (Hibiscus syriacus), Fubisu (Hibiscus coccineus), Greater sea bream (Hibiscus coccineus), Great foxtail (Hibiscus) tiliaceus) and Bussougi (Hibiscus schizopetalus), and the like, but Roselle (Hibiscus sabdariffa L.) is preferred. Examples of the use site include whole grass, leaves, flower parts, stems, roots, grains, etc., but flower parts are preferred. When using a flower part, the flowering time, size, etc. are not particularly limited, and a flower part, a flower bud, or the like containing any or all of them may be used.

  It is preferable to carry out the fermentation treatment for Fuso plants as follows. That is, as an assimilation source of fermentation, the plant itself (hereinafter referred to as a plant) may be used, or an extract obtained from the plant by the following solvent extraction method may be used. Moreover, when using an extract, it is also possible to perform fermentation with a plant body contained, without removing the plant body of an extract by solid-liquid separation. Here, the plant may be raw or may be previously dried or semi-dried. Further, the collected shape can be used as it is.

  When the extract is used for the fermentation treatment, the extraction solvent is water; lower alcohols such as methanol, ethanol and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; ethylene glycol, 1, 3 -Polyhydric alcohols such as propanediol, 1,3-butylene glycol and glycerine; Esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; Ketones such as acetone and methyl ethyl ketone; Ethyl ether, isopropyl, Ethers such as ether; hydrocarbon solvents such as n-hexane, toluene, chloroform and the like can be mentioned, and these can be used alone or in admixture of two or more.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 in a volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. If it is 1 to 15: 1, or a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, it is preferably in the range of 1: 1 to 15: 1.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C. to 90 ° C., and the extraction time is preferably 0.5 hours to 7 days.

  In the present invention, examples of microorganisms used for fermentation include lactic acid bacteria, yeast, koji molds, and Bacillus subtilis.

  In the present invention, lactic acid bacteria are, for example, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbrueckii Lactobacillus delbrueckii and other Lactobacillus genus Lactobacillus genus Lactobacillus Lactic acid bacteria of the genus Carnobacterium such as Carnobacterium divergens, Carnobacterium piscicola; Leuconostoc mesenteroides, Leuconostoc lactis, Leuconostoc lactis Leuconostoc lactic acid bacteria such as Leuconostoc citreum; Streptococcus faecalis, Streptococcus pyogenes (St Leptococcus pyogenes) and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus sulfreus and other Enterococcus lactic acid bacteria; Lactococcus lacta Lactic acid bacteria of the genus Lactococcus; lactic acid bacteria of the genus Weissella such as Weissella confusa and Weissella kandleri; Lactic acid bacteria of the genus Vagococcus (Vagococcus fluvialis), Vagococcus salmoninarum, etc .; Pedi Lactococcus Damunosasu (Pediococcus damnosus), include lactic acid bacteria of the Pediococcus (Pediococcus) genus such as Pediococcus pentosaceus (Pediococcus pentosaceus).

  Further, in the present invention, yeast refers to, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carls bergensis, Yeast of the genus Saccharomyces, yeasts of the genus Galactomyces; yeasts of Schizosaccharomyces pombe such as Schizosaccharomyces pombe; Torulaspora delbruekii, Torulaspora rosei) and other yeasts, Zygosaccharomyces rouxii, Zygosacchar omyces soya, Zygosaccharomyces soya, Zygosaccharomyces soya accharomyces sake), Zygosaccharomyces miso, Zygosaccharomyces lactis and other yeasts of the genus Gigosaccharomyces, Candida versatilis, Candida etc. (Candida sake), Candida scottii and other Candida yeasts, Aureobasidium Pullulans, Aureobasidium mansonii, Aureobasideium microstictum Aureobasidium yeast and the like. The yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, plant flower (rose, lily, cherry tree, etc.) yeast, or sea-derived yeast. The yeast etc. are mentioned.

  Further, in the present invention, aspergillus refers to, for example, Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus sojae, etc. ), Aspergillus kawauchii, Aspergillus usami, Aspergillus niger, etc. Aspergillus niger, Monascus anka, Monascus pilosus, etc.

  In the present invention, examples of Bacillus subtilis include Bacillus natto, Bacillus subtilis, and Bacillus circulans.

  When the above-mentioned suspension or extract is fermented with microorganisms, it is necessary to sterilize the microorganisms before the fermentation step to remove the germs that hinder fermentation. As a method for sterilizing and removing the various bacteria, a method in which the fermentation material is washed with ethanol for sterilization in advance and then suspended in a sterile solvent such as sterile water may be used. The turbid liquid may be sterilized by heat sterilization or the like. As the heat sterilization treatment, the autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, or holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. The heat sterilization method such as the method is generally used.

The sterilized suspension is placed in a fermentation tank, in which microorganisms are inoculated and fermented. The appropriate amount of inoculated microorganism is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably 20 ° C. to 40 ° C., which is the optimum temperature for growth of each microorganism (for example, 30 ° C. to 40 ° C. for lactic acid bacteria, 25 ° C. to 30 ° C. for yeast). Range. The number of days of fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation days are shorter than the above general range, the fermentation is not sufficiently performed and the effectiveness of the fermented product tends to be reduced. On the other hand, if the fermentation days are longer than 10 days, no further increase in effectiveness is observed. Not only that, but also coloring and an increase in fermentation odor occur.

  When preparing a fermented product, in order to use the components of the target use site more effectively as a source of microorganism utilization, the suspension or extract solution is used before or simultaneously with inoculation of the microorganism. Then, the hydrolysis treatment may be performed with acid, alkali or enzyme.

  In general, the extract or fermented product prepared as described above may be used as it is after adjusting the pH to 3 to 9, or may be used as a desired concentration by vacuum concentration or the like. Also good. Moreover, it is good also as a dried material by conventional methods, such as a spray-dry method.

  In addition, the extract or fermented product prepared as described above may be stored after refrigeration for a certain period of time in order to enhance storage stability and the like, and then the supernatant may be used.

  Examples of preparations that can be blended with the fermented product according to the present invention (cosmetics or quasi-drugs, hereinafter referred to as “cosmetics etc.”) include, for example, emulsions, creams, lotions, essences, packs, lipsticks, foundations, and liquids. Examples include foundations, makeup press powders, cheeks, white powders, facial cleansers, body creams, body shampoos, soaps, bath preparations and the like, but of course not limited thereto.

  The amount of the extract or fermented product of the present invention to be blended in cosmetics and the like is 0.0002 to 3.0% by weight (solid content% by weight, the same applies hereinafter), makeup in the case of basic cosmetics. In the case of up cosmetics, it is generally 0.002 to 1.0% by weight, and in the case of cleaning cosmetics, it is generally 0.002 to 10.0% by weight.

  When the present invention is blended in cosmetics and the like, in addition to the extract or fermented product that is the active ingredient, components usually used in cosmetics, such as oily components, surfactants (synthetic systems, natural products systems) ), A moisturizer, a thickener, an antiseptic / bactericidal agent, a powder component, an antioxidant, a chelating agent, a pH adjuster, a pigment, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and the characteristics of the extract according to the present invention are not impaired, it may be combined with other physiologically active ingredients.

  Here, as the oil component, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, palm oil, chamomile oil, palm oil, cacao oil, meadow foam oil, rose Oils derived from plants such as hip oil, lambender oil, sheer butter, tea tree oil, avocado oil, macadamia nut oil, plant-derived squalane; oils derived from animals such as mink oil and turtle oil; beeswax, carnauba wax, rice wax , Waxes such as lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; lauryl alcohol, cetanol, stearyl Higher alcohols such as alcohol; isopropyl myristate , Isopropyl palmitate, butyl oleate, 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components; silane root (white) extract; pectin, locust bean gum, aloe polysaccharides, alkaligenes-producing polysaccharides, etc. Polysaccharides; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / disinfectant include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzal chloride Plants such as Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), Polyphosphoric acid, Propanediol, 1,2-Pentanediol, Various essential oils, Bark distillate, Radish fermentation liquor, Sugar cane Origin of ethanol or 1,3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, cereal (rice, wheat, corn, millet, etc.) powder, legume (soybean, red bean, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, murasakixibub extract, silane root extract, peony extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc. ) Etc.

  Examples of chelating agents include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, and tetrasodium hydroxyethanediphosphonate.

  Examples of the pH adjuster include citric acid or a salt thereof, lactic acid or a salt thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like.

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as prillyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxy. Cibenzoic acid and the like, nicotinic acid derivatives include, for example, nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid, and the like. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, pearl barley hydrolyzate, barley seed fermented product, royal jelly fermented product, sake lees extract or ceramide contained therein Examples thereof include fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe, etc. Of seaweed extract, soymilk fermented product, jellyfish water, eel extract, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzed product thereof, germinated rice fermented product, damask rose flower Extract, bamboo shoot extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt) Etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hy Droxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, red ginseng extract, beetroot extract, loofah extract, anaaosa extract, peach extract, peach extract, kiwi extract , Sunflower extract, Zizyphus joazeiro extract, pawdarco bark extract, dairy lily extract or fermented product, hagoromogusa extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea Extract, red rich extract, silane extract, extract or hydrolyzate of yam peel or seed coat, safflower flower extract, Casablanca extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, Evening birch extract, aloe extract, fig flower extract, apple extract, white asparagus extract etc. That.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of fermented hibiscus 950 g of purified water was added to 50 g of flower parts of hibiscus (Hibisucas sabdariffa L.) to make a suspension, and the mixture was heated at 80 ° C. for 1 hour for sterilization. The sterilized suspension was inoculated with 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum), and statically cultured at 37 ° C for 3 days. After completion of the culture, the culture solution was sterilized by heating and filtered to obtain 730 g of a lactic acid bacteria fermented product solution (solid content concentration: 3.21%).

Test Example 1 Outer hair root sheath cell growth inhibitory effect Human outer root sheath cell HHORSC was seeded at 2 × 10 ³ cells / hole in a 96-well microplate containing MSCM medium (manufactured by ScienCell Research Laboratories), 37 ° C., 5.0% CO After pre-culturing for 1 day under the conditions of ₂ , a medium containing the solution prepared based on Production Example 1 as a sample solution was added, and further cultured for 2 days under the same conditions. Here, the concentration of the sample solution was adjusted so that the final concentration as a solution was 1.0% and 2.0% with respect to the total amount of the medium. After culturing for 2 days, the supernatant was discarded, washed once with PBS (−), 100 μL / well of hoechst33342 reagent diluted 100-fold with PBS (−) was added, incubated at 37 ° C. for 1 hour, and DNA was fluorescently stained. . Thereafter, fluorescence intensity (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader [Fluoroscan Ascent (manufactured by Thermo Fisher Scientific)]] was measured to determine the amount of DNA. 30% 1,3 instead of sample solution -For the group to which butylene glycol was added, the group in which the same operation was performed was used as a control, and the relative value of the fluorescence intensity of each sample addition group with respect to the fluorescence intensity (DNA amount) obtained here was determined, and the cell growth rate (%).

The results of Test Example 1 are shown in Table 1.

  As shown in Table 1, it was confirmed that the fermented product according to the present invention has an outer hair root sheath cell growth inhibitory effect. That is, since the fermented product according to the present invention exhibits an outer hair root sheath cell growth inhibitory effect, it is considered that the growth of hair matrix cells that are the same hair follicle epithelium-derived cells is also suppressed. Therefore, the hair elongation inhibiting effect was suggested, and it was confirmed that it was useful as a skin external preparation for suppressing hair growth (for hair suppression). Furthermore, it can also be applied to a skin external preparation for hair removal or hair removal.

Formulation Example 1 Lotion [Ingredients] part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Fermented product of Production Example 1 3.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total

Formulation Example 2 Latex [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Fermented product of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 3 Emulsion [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Fermented product of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 4 Lotion [Component] part Fermented product of Production Example 1 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total

Formulation Example 5 Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Fermented product of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water totaling 100 parts

Formulation Example 6 Body shampoo [ingredient] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Fermented product of Production Example 1 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Formulation Example 7 Cream [ingredient] part olive oil 5.0
Squalane 5.0
Jojoba oil 5.0
Jojoba wax 3.0
Behenil alcohol 1.0
Stearyl alcohol 1.5
Candelilla wax 0.5
Lactic acid bacteria fermented rice 2.0
Fermented product of Production Example 1 1.0
Hydrogenated lecithin 0.5
Carboxyvinyl poly 0.3
Sodium alginate 0.2
Methylparaben 0.15
Ethylparaben 0.03
Amount of purified water totaling 100 parts

Claims (1)

  A skin external preparation for inhibiting hair growth, comprising as an active ingredient a fermented product of a plant belonging to the family Malvaceae Hibiscus.