JP7244004B2 - Skin topical agent - Google Patents

Description

TECHNICAL FIELD The present invention relates to an external preparation for skin that is excellent in biosafety and that has an excellent hair growth inhibitory effect.

Conventionally, methods for removing body hair (hands, feet, armpits, torso, face, etc.) (hair removal creams, tape stripping) have been proposed for aesthetic reasons. Problems such as unhealthy skin arose.

In view of the problems of the prior art, the present inventors have conducted intensive studies to discover new active ingredients derived from natural products from the viewpoint of biosafety. As a result, the inventors newly found that a fermented product of a plant belonging to the genus Hibiscus has an excellent hair growth inhibitory effect.

Conventionally, it has been known from Patent Documents 1 and 2 that extracts of plants belonging to the genus Hibiscus or fermented products of these plants are used in cosmetics, but it is known that these extracts and fermented products have the effect of suppressing the growth of body hair. It wasn't done.
Japanese Patent Application Laid-Open No. 57-99517 Japanese Patent Application Laid-Open No. 2006-347925

The present invention is a skin external preparation for suppressing the growth of body hair, containing a fermented product of a plant belonging to the genus Hibiscus as an active ingredient.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide an external preparation for skin that is excellent in biosafety and has an excellent effect of inhibiting the growth of body hair.

Preferred embodiments of the present invention are described in detail below.
Plants belonging to the genus Hibiscus of the family Malvaceae used in the present invention include, for example, roselle (Hibiscus sabdariffa L.), Hibiscus syriacus, Hibiscus mutabills, Hibiscus coccineus, and Hibiscus. tiliaceus), Bussouge (Hibiscus schizopetalus) and the like, but Roselle (Hibiscus sabdariffa L.) is preferred. The parts to be used include whole plants, leaves, flower parts, stems, roots, grains, etc. Flower parts are preferred. When flower parts are used, their flowering time and size are not particularly limited, and either or all of petals, sepals, etc. may be used.

Fermentation treatment of Hibiscus genus plants is preferably carried out as follows. That is, the plant itself (hereinafter referred to as plant body) may be used as the resource for fermentation, or an extract obtained from the plant body by the following solvent extraction method may be used. Moreover, when using an extract, it is also possible to carry out fermentation while containing the plant body without removing the plant body of the extract by solid-liquid separation. Here, the plant may be fresh or pre-dried or semi-dried. As for the shape, it is also possible to use the sampled one as it is.

When the extract is used for fermentation treatment, the extraction solvent includes water; lower alcohols such as methanol, ethanol and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; -polyhydric alcohols such as propanediol, 1,3-butylene glycol and glycerin; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethyl ether, isopropyl, ethers such as ether; hydrocarbon solvents such as n-hexane, toluene and chloroform;

When a mixed solvent is used, the mixing ratio is, for example, 1:1 to 25:1 by volume (hereinafter the same) for a mixed solvent of water and ethyl alcohol, and 1:1 for a mixed solvent of water and glycerin. It is preferably in the range of 1 to 15:1, or in the case of a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, it is in the range of 1:1 to 15:1.

When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, and the desired may be adjusted to a pH of

Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. If so, the extraction temperature is preferably in the range of 0° C. to 90° C. and the extraction time is preferably in the range of 0.5 hours to 7 days.

In the present invention, microorganisms used for fermentation include lactic acid bacteria, yeast, koji mold, and bacillus subtilis.

In the present invention, lactic acid bacteria are lactic acid bacteria of the genus Lactobacillus, such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbrueckii; Carnobacterium divergens, Carnobacterium piscicola and other lactic acid bacteria of the genus Carnobacterium; Leuconostoc mesenteroides, Leuconostoc lactis, Leuco Lactic acid bacteria of the genus Leuconostoc such as Leuconostoc citreum; Lactic acid bacteria of the genus Streptococcus such as Streptococcus faecalis and Streptococcus pyogenes; lactic acid bacteria of the genus Enterococcus such as sulfreus; lactic acid bacteria of the genus Lactococcus such as Lactococcus plantarum; Lactococcus rafinolactis; Weissella confusa, Weissella kandleri, etc. Lactic acid bacteria of the genus Veythera; Lactic acid bacteria of the genus Atopobium such as Atopobium minutum and Atopobium parvulus; Vagococcus fluvialis, Vagococcus salmon inarum); Pediococcus lactic acid bacteria such as Pediococcus damnosus and Pediococcus pentosaceus.

In the present invention, yeast includes, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, Saccharomyces bayonus, etc. yeast of the genus Saccharomyces, yeast of the genus Galactomyces; yeast of Schizosaccharomyces such as Schizosaccharomyces pombe; Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei Zygosaccharomyces rouxii, Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, Zygosaccharomyces lactis, etc. Yeast of the genus Zygosaccharomyces, Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, Candida scottii, etc., Aureoba Yeast of the genus Aureobasidium such as Aureobasidium Pullulans, Aureobasidium mansonii, and Aureobasideium microstictum. The yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, yeast derived from plant flowers (rose, lily, cherry, etc.), and yeast derived from the sea. and the like.

In the present invention, koji mold includes, for example, yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus awamori ), black aspergillus such as Aspergillus kawauchii, Aspergillus usami, Aspergillus niger, and red aspergillus such as Monascus anka and Monascus pilosus.

In the present invention, Bacillus subtilis includes, for example, Bacillus natto, Bacillus subtilis, Bacillus circulans and the like.

When the suspension or extract described above is fermented with microorganisms, it is necessary to sterilize the suspension or extract before the fermentation process to remove germs that hinder fermentation. As a method for sterilizing and removing various germs, a method may be used in which the fermented material is washed in advance with sterilizing ethanol or the like and then suspended in a sterile solvent such as sterile water. The turbid liquid may be sterilized by heat sterilization or the like. As the heat sterilization treatment, there is an autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, and an intermittent sterilization that repeats holding at 80 to 90 ° C. for 60 to 120 minutes once a day for 2 to 3 days. Heat sterilization methods such as the method are commonly used.

The sterilized suspension is placed in a fermentation tank, inoculated with microorganisms and fermented. A proper amount of microorganisms to be inoculated is 10 ⁷ to 10 ⁸ cells/mL. Even if the amount of inoculum exceeds the above range, the progress time of fermentation hardly changes.

Fermentation temperature is generally in the range of 5 to 50° C., preferably 20° C. to 40° C., which is the optimum growth temperature for each microorganism (for example, 30° C. to 40° C. for lactic acid bacteria, 25° C. to 30° C. for yeast). is in the range of Fermentation days are generally in the range of 1 to 10 days, preferably 2 to 5 days at the optimum temperature. If the number of days for fermentation is shorter than the above general range, the fermentation will not be carried out sufficiently and the efficacy of the fermented product will tend to decrease. In addition, coloring and an increase in fermented odor are caused, which are both undesirable.

When preparing a fermented product, in order to more effectively utilize the components of the target use site as a resource for microorganisms, before or at the same time as inoculating microorganisms, the above suspension or extract solution Alternatively, hydrolysis treatment may be carried out with an acid, alkali or enzyme.

The extract or fermented product prepared as described above may generally be used as it is after adjusting the pH to 3 to 9, or it may be used at a desired concentration by vacuum concentration or the like. Also good. Alternatively, it may be dried by a conventional method such as a spray drying method.

In addition, the extract or fermented product prepared as described above may be stored in a refrigerator for a certain period of time and then the supernatant may be used in order to improve storage stability.

Examples of formulations (cosmetics or quasi-drugs, hereinafter referred to as "cosmetics, etc.") that can be blended with the fermented product according to the present invention include emulsions, creams, lotions, essences, packs, lipsticks, foundations, and liquids. Foundation, make-up press powder, blusher, white powder, facial cleanser, body cream, body shampoo, soap, bath agent, etc., but are of course not limited to these.

The amount of the extract or fermented product of the present invention to be blended in cosmetics, etc., is 0.0002 to 3.0% by weight (solid content by weight, the same applies hereinafter) in the case of basic cosmetics, as the solid content. In the case of makeup cosmetics, it is generally 0.002 to 1.0% by weight, and in the case of cleansing cosmetics, it is generally 0.002 to 10.0% by weight.

When the present invention is blended into cosmetics, etc., in addition to the extract or fermented product, which is the active ingredient, ingredients that are usually used in cosmetics, such as oily ingredients, surfactants (synthetic, natural products, etc.) ), moisturizing agents, thickeners, antiseptic/bactericidal agents, powder components, antioxidants, chelating agents, pH adjusters, pigments, perfumes, etc. can be appropriately blended as necessary. In addition, the extract of the present invention may be blended in combination with other physiologically active ingredients as long as the effectiveness and features of the extract are not impaired.

Examples of oily components include lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadowfoam oil, and rose oil. Plant-derived fats and oils such as hip oil, lanvender oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; beeswax, carnauba wax, rice wax , Waxes such as lanolin; Hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane; Fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; Lauryl alcohol, cetanol, stearyl higher alcohols such as alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexylglyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.);

Examples of surfactants include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, poly Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkylphenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, cationic surfactants such as alkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorphonium salts, polyethylenepolyamine fatty acid amide salts; N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N',N'-dimethyl-N'- Amphoteric surfactants such as β-hydroxypropylammoniosulfobetaine and the like can be used.

Examples of emulsifiers or emulsifying aids include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), complexes of sugar and protein, sucrose or its esters, lactose, soybean-derived water-soluble Polysaccharides, complexes of soybean-derived protein and polysaccharides, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented grains (barley, beans, cereals, etc.) and the like can also be blended.

Moisturizing agents include, for example, glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, and mucopolysaccharides (eg, hyaluronic acid). acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) extracts, various amino acids and derivatives thereof.

Examples of thickening agents include alginic acid, agar, carrageenan, fucoidan, and other brown algae, green algae, or red algae-derived components; Polysaccharides; gums such as xanthan gum, tragacanth gum, guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, acrylic acid/methacrylic acid copolymer; and derivatives thereof; polyglutamic acid and derivatives thereof;

Antiseptic/bactericidal agents include, for example, urea; paraoxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophen, hexachlorophene, chlorhexidine hydrochloride, benz chloride Ruconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, dry distillation of bark, fermented radish, sugar cane, etc. ethanol or 1,3-butylene glycol derived from

Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose. cereals (rice, wheat, corn, millet, etc.) powders, beans (soybeans, azuki beans, etc.) powders, etc.

UV absorbers include, for example, ethyl para-aminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, purslane extract, sycamore root extract, peony extract, vitamin E and derivatives thereof (e.g., vitamin E nicotinate, vitamin E linoleate, etc.). ), etc.

Examples of chelating agents include trisodium ethylenediaminehydroxyethyltriacetate, edetic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethanediphosphonate, and the like.

Examples of pH adjusters include citric acid or salts thereof, lactic acid or salts thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide and potassium hydroxide.

Whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone and its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, - methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate) , adenosine monophosphate), and these may be blended singly or in combination.

Examples of the above kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate and kojic acid dibutyrate; kojic acid ethers; kojic acid sugar derivatives such as kojic acid glucoside; However, ascorbic acid derivatives include, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl potassium phosphate, myristyl 3-glyceryl ascorbate, caprylyl Ascorbic acid sugar derivatives such as 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L- L-ascorbic acid tetrafatty acid esters such as ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylation derivatives, ascorbic acid glycerol derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. However, hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (eg, tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (e.g., tranexamic acid methylamide), etc., and resorcinol derivatives such as 4-n-butylresorcinol and 4-isoamylresorcinol. etc., and 2,5-dihydroxybenzoic acid derivatives such as 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyl xybenzoic acid and the like, nicotinic acid derivatives such as nicotinamide and benzyl nicotinate, and α-hydroxy acids such as lactic acid, malic acid, succinic acid, citric acid and α-hydroxyoctanoic acid. .

Examples of physiologically active ingredients include placenta extract, saxifrage extract, saxifrage extract, perilla extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, fermented white mustard, peony extract or hydrolyzate thereof, lactic acid fermented rice, lotus seed extract or hydrolyzate thereof, fermented lotus seed, hydrolyzate of adlay, fermented adlay seed, fermented royal jelly, sake lees extract or ceramide contained therein, Sake lees fermented product, Pandanus amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. In addition, coral grass extract, rice leaf extract or its hydrolyzate, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, eucheuma cornflower, etc. seaweed extract, fermented soymilk, jellyfish water, eelgrass extract, rice extract or its hydrolyzate, fermented rice extract, germinated rice extract or its hydrolyzate, fermented germinated rice, damask rose flower extract, bamboo shoot skin extract, linoleic acid and its derivatives or processed products (e.g., liposomal linoleic acid), animal- or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, Panax ginseng extract or its fermented product , red ginseng extract, honey kelp extract, luffa extract, sea lettuce extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, Pau d'Arco bark extract, daylily ) extracts or fermented products, wormwood extracts, cherimoya extracts, mango extracts, mangosteen extracts, funori extracts, oolong tea extracts, Benifuki extracts, sylvan extracts, extracts or hydrolysates of Japanese pepper pericarp or seed coats safflower flower extract, Casablanca extract, sweet potato extract or its fermented product, guava leaf extract, houttuynia cordata extract, banpeiyu extract, aloe extract, fig flower extract, apple extract, white asparagus There are extracts and the like.

Next, the present invention will be described in more detail with production examples, formulation examples and test examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % means weight %.

Production example 1. Preparation of Fermented Hibiscus Product 950 g of purified water was added to 50 g of flower parts of hibiscus (Hibisucas sabdariffa L.) to form a suspension, which was then heated at 80° C. for 1 hour for sterilization. The sterilized suspension was inoculated with 10 ⁸ cells/mL of lactic acid bacteria (Lactobacillus plantarum) and statically cultured at 37° C. for 3 days. After completion of the cultivation, the culture solution was sterilized by heating and filtered to obtain 730 g of lactic acid fermented product solution (solid concentration: 3.21%).

Test example 1. Outer Root Sheath Cell Proliferation Suppressive Effect Human outer root sheath cells HHORSC were seeded in a 96-well microplate containing MSCM medium (manufactured by ScienCell Research Laboratories) at 2×10 ³ cells/well, and treated at 37° C., 5.0% CO. After preculturing for 1 day under the conditions of _{No. 2} , a medium containing the solution prepared according to Production Example 1 as a sample solution was added, and the cells were further cultured under the same conditions for 2 days. Here, the concentration of the sample solution was adjusted so that the final concentration as a solution was 1.0% and 2.0% with respect to the total amount of the medium. After culturing for 2 days, the supernatant was discarded, and after washing once with PBS(-), 100 μL/well of hoechst33342 reagent diluted 100-fold with PBS(-) was added, incubated at 37° C. for 1 hour, and the DNA was fluorescently stained. . After that, fluorescence intensity (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader [Fluoroscan Ascent (manufactured by Thermo Fisher Scientific)] was measured to determine the amount of DNA.30% 1,3 instead of sample solution - The group to which the butylene glycol was added was also subjected to the same operation as a control, and the relative value of the fluorescence intensity of each sample addition group to the obtained fluorescence intensity (DNA amount) was calculated, and the cell growth rate was calculated. (%).

Table 1 shows the results of Test Example 1.

As shown in Table 1, it was confirmed that the fermented product according to the present invention has an effect of inhibiting the proliferation of outer root sheath cells. That is, since the fermented product according to the present invention exhibits an effect of suppressing proliferation of outer root sheath cells, it is considered that it also suppresses proliferation of hair matrix cells, which are also cells derived from the hair follicle epithelium. Therefore, the effect of suppressing hair elongation was suggested, and it was confirmed that it is useful as an external skin preparation for suppressing the growth of body hair (for suppressing hair). Furthermore, it can also be applied to skin external preparations for depilation or depilation.

Formulation example 1. Lotion [Ingredients] Part Olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Fermented product of Production Example 1 3.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 2. Emulsion [Ingredients] Part Liquid paraffin 6.0
Hexalane 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Fermented product of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts

Formulation example 3. Emulsion [Ingredients] Part Liquid paraffin 6.0
Hexalane 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Fermented product of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount that makes the total amount 100 parts

Formulation example 4. Lotion [Ingredients] Part Fermented product of Production Example 1 5.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Potassium hydroxide Appropriate amount Purified water Amount to make the total amount 100 parts

Formulation example 5. Essence [ingredient] part ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Fermented product of Production Example 1 5.0
Citric acid 0.3
Sodium citrate 0.6
Purified water Amount that makes the total amount 100 parts

Formulation example 6. Body Shampoo [Ingredients] Part N-lauroylmethylalanine sodium 25.0
Coconut fatty acid potassium liquid (40%) 26.0
Coconut fatty acid diethanolamide 3.0
Methylparaben 0.1
Fermented product of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water Amount that makes the total amount 100 parts

Formulation example 7. Cream [Ingredients] Part Olive oil 5.0
Squalane 5.0
Jojoba oil 5.0
Jojoba wax 3.0
behenyl alcohol 1.0
Stearyl alcohol 1.5
Candelilla wax 0.5
Lactic acid bacteria fermented rice 2.0
Fermented product of Production Example 1 1.0
Hydrogenated lecithin 0.5
Carboxy vinyl poly 0.3
Sodium alginate 0.2
Methylparaben 0.15
Ethylparaben 0.03
Purified water Amount that makes the total amount 100 parts

Claims (1)

An external skin preparation for inhibiting body hair growth containing, as an active ingredient, a lactic acid bacterium ( Lactobacillus plantarum) fermented product of the flower part of Hibiscus (Hibisucas sabdariffa L.) belonging to the genus Hibiscus of the family Malvaceae.