JP2021172593A - External preparation for skin - Google Patents

Abstract

To provide an active ingredient for an external preparation for skin, which is derived from a natural product and has excellent biosafety, as well as prevents and improves skin damage or aging caused by ultraviolet rays and the like, and keeps the skin in a youthful and healthy condition.SOLUTION: The external preparation for skin of the present invention comprises a fermented product of a plant belonging to Hibiscus mutabilis and an extract of a plant belonging to Rosaceae Rosa as active ingredients.SELECTED DRAWING: Figure 1

Description

The present invention is a skin containing a combination of an extract of a plant belonging to the Rosaceae (Rosaceae) genus Rosa (Rosa) and a fermented product of a plant belonging to the genus Hibiscus of the Malvaceae (Malvaceae) as an active ingredient. Regarding external preparations.

In recent years, from the viewpoint of biosafety, various plant-derived components have been developed as compounding components for external skin preparations. However, plant-derived components have problems in terms of effectiveness and stability. In particular, in the case of plant-derived components, there is a problem that they may not exhibit sufficient effectiveness by themselves.

In view of the problems of the prior art, the present inventors, the combination of the fermented product of the plant belonging to the genus Confederate rose in the Malvaceae family and the extract of the plant belonging to the genus Rosaceae in the family Rosaceae has an excellent synergistic effect of skin improvement. It was newly found that it has.

Conventionally, a skin external preparation containing a fermented product of a plant belonging to the genus Confederate rose in the Malvaceae family as an active ingredient is known from Patent Document 1, for example, and an extract of a rose belonging to the genus Rosaceae in the family Rosaceae is used as an active ingredient. The skin external preparation to be used is known, for example, in Patent Documents 2 to 6.

Japanese Patent Application Laid-Open No. 2006-347925 Japanese Patent Application Laid-Open No. 2000-327555 Japanese Patent Application Laid-Open No. 2001-064192 JP 2001-163794 JP 2001-316277 Japanese Patent Application Laid-Open No. 2014-240375

However, it has not been known that the combination of a fermented product of a plant belonging to the genus Confederate rose in the Malvaceae family and an extract of a plant belonging to the genus Rosaceae has a synergistically excellent skin improving effect.

The present invention is an external preparation for skin containing a fermented product of a plant belonging to the genus Confederate rose of the Malvaceae family and an extract of a plant belonging to the genus Rosaceae of the Rosaceae family.

The present invention is excellent in biosafety and effectiveness by using a combination of a fermented product of a plant belonging to the genus Confederate rose in the Malvaceae family and an extract of a plant belonging to the genus Rosaceae in the active ingredient, and has a synergistic effect of skin improvement. It is possible to provide a skin external preparation that exerts its effect.

It is a figure which shows the evaluation test result of the epidermal cell activation effect. It is a figure which shows the evaluation test result of the active oxygen scavenging effect in the epidermal cell. It is a figure which shows the evaluation test result of the anti-glycation effect.

Hereinafter, preferred embodiments of the present invention will be described in detail.
First, fermented products of plants belonging to the genus Confederate rose (Hibiscus) in the Malvaceae family will be described. Examples of plants belonging to the genus Hibiscus in the family Hibiscus include Rosel (Hibiscus sabdariffa L.), Hibiscus syriacus, Hibiscus mutabills, Hibiscus coccineus, Hibiscus tiliaceus, and Hibiscus ros. , Hibiscus schizopetalus. The site of use of plants of the genus Confederate rose (Hibiscus) is not particularly limited, and appropriate parts such as whole plants, leaves, stems, flowers, calyxes, calyxes, female calyxes, stems, roots, seeds, and grains can be used. , Whole plant, flower, calyx are preferred.

As the assimilation source used for the fermentation of plants of the genus Confederate rose, the plant itself (hereinafter referred to as a plant body) may be used, or an extract obtained from the plant body by a solvent extraction method described later may be used. In addition, when an extract is used, it is possible to carry out fermentation while containing the plant without removing the plant to be extracted by solid-liquid separation. Here, the plant may be raw or pre-dried or semi-dried. Moreover, it is also possible to use the collected one as it is as the shape.

In the present invention, examples of the microorganism used for fermentation of plants of the genus Hibiscus include lactic acid bacteria, bifidobacteria, aspergillus, natto bacteria, tempeh bacteria, yeast and the like, and are generally selected from any of these bacterial species. One kind or two or more kinds are used, but in some cases, those belonging to different bacterial species may be used in combination as long as they do not interfere with each other's fermentation.

For example, examples of lactic acid bacteria include Lactobacillus plantarum, L. brevis, L. casei, L. delbrueckii and other Lactobacillus genus lactic acid bacteria; Lactobacillus genus Carnobacterium such as Carnobacterium divergens and Carnobacterium piscicola; Leuconostoc mesenteroides such as Leuconostoc citreum Lactobacillus of the genus Leuconostoc; Lactobacillus of the genus Streptococcus such as Streptococcus faecalis and Streptococcus pyogenes; Lactobacillus lactic acid bacteria such as Lactococcus plantarum and Lactococcus rafinolactis; Weissella confusa, Weissella kandleri and other lactic acid bacteria of the genus Weissella kandleri; Lactobacillus of the genus Atopobium such as minutum) and Atopobium parvulus; Vagococcus fluvialis, Vagococcus salmoninarum, etc. Pedi Examples include lactic acid bacteria of the genus Pediococcus such as Pediococcus pentosaceus, and lactic acid bacteria of marine origin such as Marinalactobacillus phychrotolerans.

For example, examples of bifidobacteria include Bifidobacterium bifidum and Bifidobacterium breve, but any of them can be used as long as they are classified as bifidobacteria. be.

Aspergillus aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus aspergillus, Aspergillus aspergillus, Aspergillus aspergillus, Aspergillus aspergillus, Aspergillus aspergillus Aspergillus kawauchii), Aspergillus usami, Aspergillus niger and other black aspergillus, Monascus anka, Monascus pilosus and other aspergillus.

Examples of Bacillus natto include bacteria of the genus Bacillus such as Bacillus natto, Bacillus subtilis, and Bacillus circulans. Among them, Bacillus natto is the most preferable because it is widely used in foods and has high safety.

Examples of Tempe bacteria include Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus nibeus The genus Rhizopus (Rhizopus) can be mentioned.

Examples of yeast include Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces cerevisiae, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis, Saccharomyces carlsbergensis , Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei and other Saccharomyces genus yeast, Zygosaccharomyces rouxii, Zygosaccharomyces rouxii, Zygosaccharomyces rouxii ), Zygosaccharomyces miso, Zygosaccharomyces lactis and other Zygosaccharomyces lactis, Candida versatilis, Candida eccharomyces, Candida, etc. Sake), Candida scottii and other Candida yeasts, Aureobasidium Pullulans, Aureobasidium mansonii, Aureobasideium microstictum, etc. Examples include yeasts of the genus Aureobasidium. Among the above-mentioned yeasts, Saccharomyces cerevisiae is preferable from the viewpoint of safety and efficacy, but Saccharomyces cerevisiae includes plant-derived yeasts such as sake, lilies, and cherry blossoms, and marine-derived yeasts. , Any origin can be used.

When the above-mentioned suspension or extract is fermented by microorganisms, sterilization is performed before the fermentation step to remove various germs that hinder fermentation. As a method for sterilizing and removing the germs, a method may be used in which the fermented material is previously washed with sterilizing ethanol or the like and then suspended in a sterile solvent such as sterile water, or the fermented material is suspended in the solvent and then suspended. The turbid liquid may be sterilized by heat sterilization or the like. The heat sterilization treatment includes an autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, and intermittent sterilization in which the suspension is held at 80 to 90 ° C. for 60 to 120 minutes once a day for 2 to 3 days. A heat sterilization method such as the method is generally used.

The sterilized suspension is placed in a fermentation tank, in which microorganisms are inoculated and fermented. The appropriate amount of microorganisms to be inoculated is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount is larger than the above range, the fermentation progress time is almost unchanged, while if it is less than the above range, it takes a long time to complete the fermentation, which is not preferable.

Preferred specific examples of the method for fermenting the above plants using the above-mentioned microorganisms are as follows. First, the fermentation materials of these plants are immersed or suspended in a fermentation medium to prepare a suspension for fermentation. In this case, the plant may be used as it is, or may be used after being dried or semi-dried in advance. Further, as the shape, the collected one can be used as it is, but the fermentation efficiency can be improved by shredding or crushing to make it finer.

As the fermentation medium for suspending the fermentation material, water or water and lower alcohols (methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol (propanediol), 1,3-butylene glycol, glycerin) Etc.), and sugars such as glucose, fructose, and shoe cloth may be added to these media, but the fact that microorganisms are most likely to exert their effects and that the fermented material is a plant. The single use of water is most preferable in the sense that it avoids the formation of fermentation by-products due to the presence of other assimilating components.

This suspension of fermented material is sterilized to remove germs that interfere with fermentation before it is subjected to the fermentation process. In this case, as a sterilization removal method, a method may be used in which the fermented material is previously washed and sterilized with sterilizing ethanol or the like and then suspended in a sterile medium such as sterile water, or the fermented material is suspended in the medium and then suspended. A method of heat sterilizing the turbid liquid may be used. Examples of the heat sterilization method include an autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, and holding the suspension at 80 to 90 ° C. for 60 to 120 minutes once a day for 2 to 3 times. Intermittent sterilization, which repeats for days, is commonly used.

Next, this sterilized suspension is placed in a fermentation tank, and microorganisms are inoculated into the suspension to carry out fermentation treatment. The appropriate amount of microorganisms to be inoculated is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount is larger than the above range, the fermentation progress time is almost unchanged, while if it is less than the above range, it takes a long time to complete the fermentation, which is not preferable.

Fermentation temperatures are generally in the range of 5-50 ° C. The number of fermentation days is generally in the range of 1 to 10 days, preferably 2 to 5 days at the optimum temperature. If the number of fermentation days is shorter than the above general range, fermentation tends to be insufficient and the effectiveness of the fermented product tends to decrease, while even if it is longer than 10 days, no further increase in effectiveness is observed. Not only that, coloring and an increase in fermented odor occur, which is not preferable.

In performing the above fermentation treatment, in order to make the components of the plant more effectively utilized by the microorganisms, the above-mentioned suspension is performed before or during the inoculation of the microorganisms, or in some cases, during the fermentation after the inoculation. An enzyme may be added to the turbid liquid to hydrolyze the plant as a fermentation material with the enzyme. In this case, as the enzyme, as described above, at least one enzyme selected from proteolytic enzyme, glycolytic enzyme, pectic degrading enzyme and lipid degrading enzyme can be used.

As for the treatment conditions such as pH, temperature, and time, if the enzyme treatment is performed before fermentation, it is preferable to perform the treatment for 1 to 24 hours near the optimum pH and temperature of the enzyme to be used. If it is carried out in parallel with the fermentation, the conditions may be the same as those of the fermentation.

When the above fermentation treatment is completed, heat sterilize the fermented liquid at 80 to 100 ° C. for about 10 to 120 minutes for sterilization of microorganisms and also for inactivation of enzymes when combined with enzyme treatment. Apply processing. The fermented liquid that has been sterilized is separated into a liquid phase as it is, or generally and preferably by a solid-liquid separation means such as filtration or centrifugation, and if necessary, the pH is set in the pH range of ordinary cosmetics. The pH is adjusted to a certain pH 4 to 9, and if necessary, the concentration is adjusted to an appropriate level by dilution or concentration, and the mixture is used as a compounding raw material for cosmetics. Further, in some cases, the liquid phase after solid-liquid separation may be solidified according to a conventional method such as a spray-drying method or a freeze-drying method, and further pulverized to form a powder if necessary.

The extract material used in the present invention is a plant belonging to the genus Rosa in the family Rosaceae, and may be of any variety (including hybrids and subspecies). For example, Rosa Centifolia, Rosa Damascena, Rosa gallica, Rosa moschata, Rosa alba and the like can be mentioned.

The material used in the present invention may be whole plant, leaf, flower part, stem, seed, fruit, root, placentation, etc. as the rose genus plant, but the whole plant or flower part may be used. preferable.

To prepare the extract, first, plants of the genus Rose (for example, whole plant, flower part, etc.) are washed with water in advance to remove foreign substances, if necessary, and then left as it is or dried, and then chopped or chopped as necessary. The mixture is pulverized and brought into contact with an extraction solvent for extraction. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as a dipping method, but a supercritical extraction method can also be used in addition to the dipping method.

Extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like. Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in admixture of two or more. Used.

Among these extraction solvents, water, lower alcohols, polyhydric alcohols, etc. are used in the present invention from the viewpoint of skin irritation and effectiveness, and from the viewpoint that they can be widely applied to cosmetics. A hydrophilic solvent is suitable. Preferred examples of using this hydrophilic solvent include, for example, water, lower alcohols (particularly ethanol), or polyhydric alcohol (particularly 1,3-butylene glycol) used alone, or water and lower alcohols. The use of a mixed solvent with (particularly ethanol) or a mixed solvent with water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin) can be mentioned. Among them, water alone or water and 1,3 -A mixed solvent of butylene glycol is particularly preferable.

When a mixed solvent is used, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, the volume ratio (hereinafter the same applies) is 1: 10 to 20: 1, and the mixed solvent of water and ethanol is used. If there is, it is preferably in the range of 1: 10 to 25: 1, and in the case of a mixed solvent of water and glycerin, it is preferably in the range of 1: 10 to 20: 1.

The weight ratio of the dried part (for example, whole plant or flower part) of damask rose to the extraction solvent is preferably in the range of 1: 1 to 1:50.

When preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkali adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acid adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent, as desired. The pH may be adjusted to the above.

Extraction conditions such as extraction temperature and extraction time differ depending on the type and pH of the solvent used, but for example, when water or 1,3-butylene glycol or a mixed solution of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C to 80 ° C. The extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week).

Prior to the extraction treatment of the present invention, or in parallel with the extraction treatment, the extract of the genus Rose may be hydrolyzed, if necessary. As a result, there is a possibility that the storage stability of the damask rose extract can be improved and the extract as a cosmetic compounding agent can be used more effectively. Similarly, the fermented product of the Confederate rose may be hydrolyzed prior to or in parallel with the fermentation treatment of the Confederate rose plant, if necessary.

When the extract is subjected to enzyme hydrolysis treatment, the enzymes include proteolytic enzymes such as actinase, papaine and pepsin, starch degrading enzymes such as glucoamylase, α-amylase and β-amylase, cellulase, hemicellulase and pectinase. One or more selected from any of the fibrinolytic enzymes and lipolytic enzymes such as lipase may be used, but one or more selected from those enzyme groups, respectively. It is more preferable to use the above enzymes in combination.

The extracts and fermented products prepared as described above may generally have a pH adjusted to 3 to 8 and then used as they are as a cosmetic compounding agent, or may be desired by concentration under reduced pressure or the like. It may be used as a concentration. Further, the extract may be a dried product by a conventional method such as a spray-drying method.

The skin external preparation containing the extract and fermented product of the present invention is used as a skin external preparation (external pharmaceutical parts, external pharmaceutical external products and cosmetics), for example, milky lotion, cream, lotion, essence, gel, pack. , Sheet mask, lipstick, foundation, liquid foundation, make-up press powder, cheeks, white powder, pigment wash, body shampoo, slimming agent, hair shampoo, soap, etc. Bathing agents and the like can also be mentioned, but the present invention is not limited to these.

The amount of the active ingredient according to the present invention in skin external preparations (cosmetics and non-pharmaceutical products) is generally 0.0002 to 1.0% by weight (solid content weight) in the case of basic cosmetics as its solid content. %, The same applies hereinafter), preferably in the range of 0.002 to 0.2% by weight, and in the case of make-up cosmetics, generally 0.0002 to 1.0% by weight, preferably 0.002 to 0.2% by weight. In the case of cosmetics for cleaning, it is generally in the range of 0.002 to 10.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 3.0% by weight. The blending amount of the extract of the present invention in the oral composition is preferably in the range of 0.1 to 15% by weight as the solid content of the extract.

Here, examples of the oily component include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadowfoam oil, sheer butter, tea tree oil, and avocado oil. , Macademia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil and other plant-derived oils and fats such as squalane; vitamin A oil; mink oil, turtle oil and other animal-derived oils and fats; Rows such as carnauba wax, rice wax and lanolin; hydrocarbons such as liquid paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and cis-11-eicosenoic acid Classes: Higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, stearyl alcohol; Synthesis of isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) Examples thereof include esters and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene hydrogenated castor oil. Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates , Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates and other anionic surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N , N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamide propyl-N', N'-dimethyl-N' An amphoteric surfactant such as −β-hydroxypropylammoniosulfobetaine can be used.

Emulsifiers and / or emulsifying aids are derived from stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybeans. Water-soluble polysaccharides, soybean-derived protein-polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and The derivative, lecithin and its derivative (hydrogenized lecithin, etc.), lactic acid bacterium fermented rice, lactic acid bacterium fermented sprouted rice, lactic acid bacterium fermented cereals (wheat, beans, miscellaneous grains, etc.) and the like can also be blended.

As the moisturizing agent, examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, and saccharides such as trehalose and raffinose. , Mucopolysaccharides (eg, hyaluronic acid and its derivatives, hyaluronic acid fermented liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea , Higher fatty acid octyldodecyl, seaweed extract, estradiol, various amino acids and derivatives thereof.

Examples of the thickener include ingredients derived from brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae; polysaccharides such as pectin and aloe polysaccharides; gums such as tragant gum, locust bean gum, xanthan gum and guar gum; Cellulose derivatives such as methyl cellulose, hydroxyethyl cellulose and hydroxypropyl cellulose; synthetic polymers such as carrageenan polymer, alkyl-modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, alginic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives ; Polyglutamic acid and its derivatives, polyacrylic acid and the like can be mentioned.

Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camfur, dl-camfur, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin. Or there are derivatives thereof and the like.

Examples of antiseptic / bactericidal agents include urea; paraoxybenzoic acid esters such as urea; methyl benzoate or a salt thereof, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate; phenoxyethanol, dichlorophenol, hexachlorophenol. , Chlorhexidine Hydrochloride, Benzalkonium Chloride, Salicylic Acid, Sodium Salicylate, Pyrithion Zinc, Benzalkonium Chloride, Ethanol, Undecylenoic Acid, Phenols, Alkyl Isoquinolinium Bromide, Resolcin, Jamal (Imidazodeine Luurea), Isopropylmethyl Phenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokithiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark dry distillate, radish There are fermented liquids, plant-derived ethanol such as sugar cane and corn, or 1,3-butylene glycol and the like.

Cell activators include pantothenyl alcohol, menthol, dl-menthol, γ-oryzanol and the like.

Examples of the anti-acne agent include sulfur, salicylic acid or a salt thereof, Photosensitizer No. 201, pyridoxine dicaprylate and the like.

The powder components include, for example, cericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, etc. There are cellulose-based powders, grains (rice, wheat, corn, millet, etc.) powders, beans (soybeans, azuki, etc.) powders, and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzoyl, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tershally butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Examples of antioxidants include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (for example, tocopherol acetate, tocopherol nicotinic acid ester), vitamin A or its derivatives (palmitic acid). Retinol, etc.) etc.

In addition, as whitening agents, ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamins One or more selected from E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate) can be mentioned.

Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol, and examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid and 2-acetoxy-5-hydroxybenzoic acid. , 2-Hydroxy-5-propionyloxybenzoic acid and the like, and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like. Kodiic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5, 5'-dipropyl-biphenyl-2, 2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acids One or more selected from derivatives, sohakuhi extract, chamomile extract, rice bran extract hydrolyzate, yukinoshita extract and white potato extract or hydrolyzate thereof can be mentioned.

Examples of the ascorbic acid derivative include ascorbic acid esters such as ascorbic acid monobutyrate, ascorbic acid monocaplate, ascorbic acid monopalmitate, and ascorbic acid dibutyrate, ascorbic acid ethers, and ascorbic acid sugar derivatives such as ascorbic acid glucoside. Ascorbic acid derivatives include, for example, L-ascorbic acid-2-phosphate ester sodium, L-ascorbic acid-2-phosphate ester magnesium, L-ascorbic acid-2-sulfate sodium ester, L-ascorbic acid-. Ascorbic acid ester salts such as 2-ascorbic acid ester magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbic acid copheryl maleic acid, ascorbic acid copheryl phosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyle group, etc.), L-ascorbic acid tetraisopalmitic acid ester, L L-ascorbic acid tetrafatty acid esters such as -ascorbic acid tetralauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acyl Chemical derivatives, glucerin derivatives of ascorbic acid such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbic acid salt As the hydroquinone derivative, albutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as the tranexamic acid derivative, tranexamic acid ester (for example, tranexamic acid lauryl ester) , Tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, methylamide tranexamic acid) and the like, and examples of the resorcinol derivative include 4-n-butylresorcinol and 4-isoamyl. Examples of the 2,5-dihydroxybenzoic acid derivative such as resorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyl. Oxybenzoic acid and the like are nicotinic acid derivatives such as nicotinic acid amide (niacinamide) and benzyl nicotinate, and α-hydroxy acids are, for example, lactic acid, malic acid, succinic acid, citric acid and α-hydroxyoctane. There is acid etc.

Next, the present invention will be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Preparation of fermented products of Confederate rose (1)
Add 950 g of purified water to 50 g of dried Confederate rose (Roselle) flowers and calyx to make a suspension, neutralize with alkali to bring the pH to around 6, and then add at 80 to 90 ° C for 1 hour. It was warmed and sterilized. Lactic acid bacteria (Lactobacillus plantarum) in sterile suspension was inoculated 10 ⁸ / mL, and allowed to stand for 3 days incubation at 37 ° C.. After completion of the culture, the culture solution was sterilized by heating and then filtered to obtain 735 g (solid content concentration 3.30%) of a fermented lactic acid bacterium solution.

Production example 2. Preparation of fermented products of Confederate rose (2)
A lactic acid bacterium fermented product solution (645 g, solid content concentration: 2.51%) was obtained in the same manner as in Production Example 1 except that the flowers and calyxes of Hibiscus syriacus were used instead of roselle.

Production example 3. Preparation of fermented products of Confederate rose (3)
810 g (solid content concentration 3.21%) of a yeast fermented product solution was obtained in the same manner as in Production Example 1 except that Saccharomyces cerevisiae, which is a yeast, was used instead of lactic acid bacteria.

Production example 4. Preparation of extract solution of Rosaceae plant (1)
The petals of Rosa Damascena (Rosaceae) were dried, 225 g of purified water was added to 15 g of the dried product, and the mixture was immersed at 4 ° C., and then 225 g of 1,3-butylene glycol was added. This was extracted at 4 ° C. and then filtered to obtain 387 g of a brown transparent flower extract solution (solid content concentration 1.03%).

Production example 5. Preparation of extract solution of Rosaceae plant (2)
390 g of a brown transparent flower extract solution was carried out by the same process as in Production Example 1 except that the petals of Rosa Centifolia were used instead of the petals of Rosa Damascena in Production Example 4. Was obtained (solid content concentration 1.05%).

Production example 6. Preparation of extract solution of Rosaceae plant (3)
380 g of a brown transparent flower extract solution was obtained by the same process as in Production Example 1 except that the petals of Rosa gallica were used instead of the petals of Rosa Damascena in Production Example 4. Obtained (solid content concentration 1.00%).

Example 1. Preparation of composition The combination of the extract of the genus Rose and the fermented product of the genus Confederate rose is, for example, the extract of any of Production Examples 1 to 3 described above and the fermented product of any of Production Examples 4 to 6. Can be prepared by mixing. The mixing ratio is preferably 1:10 to 10: 1 in terms of solid content ratio with respect to the extract of the genus Rose: the fermented product of the genus Confederate rose.

[Table 1]

It was confirmed that all the compositions shown in Table 1 had no coloring, odor and precipitation for 1 month at 0 ° C., room temperature and 40 ° C., and were confirmed to be excellent as active ingredients of external skin preparations. rice field.

Test example 1. Evaluation test of UV damage recovery effect Human epidermal cells NHEK (F) were ^{seeded in 2 × 10 3} cells / hole in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo), 37 ° C, 5.0% CO. _{After pre-culturing under the condition of 2} for 1 day, the medium was removed, washed once with PBS (-), then PBS (-) was added, and a UV-B lamp (Philips TL20W /) was applied from the bottom of the incubator. UV irradiation of about 50 mJ / cm ^{2 was performed using 12RS).} Then, the composition 10 in Table 1 was replaced with a sample solution (sample of the present invention) added to HuMedia KB2 medium (HuMedia KG2 from which cell growth factors were removed, manufactured by Kurabou Co., Ltd.) for another 2 days under the same conditions. It was cultured. Here, the sample solution was added to the medium so that the final concentration as a solution was 0.5%. Further, instead of the composition 4, there is also a comparative test group in which a composition containing only the fermented product of Production Example 1 (comparative sample 1) or a composition containing only Production Example 4 (comparative sample 2) is added to the medium as a comparative sample solution. I set it. After culturing the cells for 2 days in the medium containing each sample solution, the medium was removed, 0.03% MTT was added and the mixture was kept at 37 ° C. for 1 hour, and then the generated formazan was extracted with isopropanol and microplates were used. The MTT value was measured at a wavelength of 570-630 nm using a reader (Model 680, manufactured by Biorad). The same operation as above was performed for the case where no sample was added (control) or for the group without UV irradiation, and the relative value of the MTT value of each treatment group to the MTT value of the UV-irradiated group was obtained and calculated as the epidermal cell activity rate (%). bottom.

The result of Test Example 1 is shown in FIG. As shown in FIG. 1, it was confirmed that the sample of the present invention had a remarkably excellent effect of recovering from UV damage to epidermal cells as compared with Comparative Sample 1 and Comparative Sample 2. From this, it was confirmed that the sample of the present invention exerts the effect of quickly recovering the damage to the skin caused by the ultraviolet rays that are exposed daily.

Test example 2. Evaluation test of active oxygen suppression effect in epidermal cells Normal human epidermal cells (NHEK) were prepared in HuMedia-KG2 medium at 8 × 10 ⁴ cells / mL, 100 μL was seeded on a 96-well microplate, and 5% carbon dioxide was used. The cells were cultured at 37 ° C. under gas and saturated steam. After 24 hours, the culture solution containing the sample was added and cultured so that the final concentration became 0.5%. In addition, a test group in which a culture solution containing BG was added so that the final concentration was 0.5% was set as a control and used as a comparison group. After 48 hours, the supernatant of the test plot was removed, 2', 7'-dichlorodihydrofluorescein diacetate (DCFH-2DA) was incorporated into the cells, washed with HBSS, and UV-B lamp (Philips) from the bottom of the incubator. Ultraviolet irradiation of ^{about 50 mJ / cm 2} was performed using TL20W / 12RS) manufactured by TL20W / 12RS. Then, the fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems), and this was used as the amount of oxidative damage. After that, Hoechst33342 was incorporated and DNA staining was performed. The fluorescence intensity (excitation wavelength 355 nm, absorption wavelength 460 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems) and used as the amount of DNA. After the measurement, the amount of oxidative damage per amount of DNA was calculated.

The result of Test Example 1 is shown in FIG. As shown in FIG. 2, it was confirmed that the sample of the present invention had a significantly superior intracellular active oxygen scavenging effect as compared with Comparative Sample 1 and Comparative Sample 2. As a result, it was confirmed that the sample of the present invention can efficiently suppress intracellular oxidative stress.

Test example 3. Evaluation test of anti-glycation effect In this test, the effect of suppressing the generation of AGE, that is, the effect of suppressing protein glycation was evaluated by coloring BSA (bovine serum albumin) via glucose. First, the composition 10 in Table 1 is used as a sample solution (sample of the present invention), and 50 μL of the sample solution, 50 μL of the 40 mg / mL BSA aqueous solution, 50 μL of the 2M glucose aqueous solution, and 50 μL of the PBS (−) solution are mixed and stirred. Prepared a sample solution. Next, the sample solution was allowed to stand at 50 ° C. for 7 days, and after 7 days, the absorbance (wavelength 405 nm: microplate reader (Model 680, manufactured by Bio-Rad)) was measured for each sample solution. Further, the same operation was performed in the case of no sample addition (control) using purified water instead of the extract of Production Example 1, and the relative value (%) of the absorbance of each sample solution to the absorbance obtained here was calculated. It was determined and used as the protein saccharification rate (%).

The results of Test Example 3 are shown in FIG. As shown in FIG. 3, it was confirmed that the sample of the present invention had a remarkably excellent anti-glycation effect as compared with the comparative sample 1 and the comparative sample 2. This suggests that it is possible to prevent yellowing of the skin due to saccharification.

Test method 4. Evaluation test of epidermal cell gene expression (1)
Normal human epidermal cells were prepared with HuMedia KG2 [manufactured by Kurabo Industries] containing a growth additive to 6 × 10 ^ 4 cells / mL, 1 mL was seeded on a 24-well plate, and 5% CO ₂ , under saturated steam, 37 ° C. Was cultured in. After culturing for 24 hours, a culture solution containing the fermented product of Production Example 1 as a sample solution (added so that the final concentration as a solution is 0.3% or 0.5% with respect to the total amount of the culture solution). Was added and cultured. As a comparative control, instead of the fermented product of Production Example 1, a culture solution containing only a 30% 1,3-butylene glycol (BG) solution (the final concentration of 30% BG with respect to the total amount of the culture solution was 0.5%. The test group (control group) to which the (adjusted to) was added was set. After culturing for 24 hours, cells in each test group were collected with 0.5 mL of Trizol reagent (manufactured by Invitrogen). 100 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, stirred and mixed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes with a centrifuge (TOMY Co., Ltd./MX-160). After that, only the aqueous layer was separated by 200 μL. 250 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer, stirred and mixed, and centrifuged at 15,000 rpm for 15 minutes under the conditions of 4 ° C. to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, stirred and washed, and the precipitate was collected by centrifugation at 15,000 rpm for 15 minutes under the condition of 4 ° C. The recovered total RNA was reverse-transcribed using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using the synthesized cDNA as a sample, using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.]. The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is commonly expressed in a certain amount in many tissues and cells, is always expressed, and is essential for cell maintenance and proliferation. It is one of the above, and since the expression level is always constant, it is used as an internal standard in PCR experiments. As for the test results, the expression level of each gene in each test group was compared when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of each gene in the other test group was determined when the expression level of each gene in the control group was 100. In this test example, gene expression of transglutaminase (TGM1), which is an enzyme involved in protein cross-linking in stratum corneum cells, and decomposition of filaggrin protein in epidermal cells to produce moisturizing factor (NHF) components. The gene expression of the involved enzyme, caspase-14 (CASP14), was evaluated.

The evaluation results of Test Example 4 are shown in Table 2.
[Table 2]

As shown in Table 2, it was confirmed that the fermented product according to Production Example 1 enhanced the expression of the transglutaminase (TGM1) gene and the caspase-14 (CASP14) gene.

Test example 5. Evaluation test of epidermal cell gene expression (2)
Normal human epidermal cells were prepared with HuMedia KG2 [manufactured by Kurabo Industries] containing a growth additive to 6 × 10 ⁴ cells / mL, 1 mL was seeded on a 24-well plate, and cultured at 37 ° C. under 5% CO2 and saturated steam. bottom. After culturing for 24 hours, a culture solution containing the extract of Production Example 4 as a sample solution (added so that the final concentration as a solution is 0.3% or 0.5% with respect to the total amount of the culture solution). Was added and cultured. As a comparative control, a culture solution containing only a 50% BG solution (the final concentration of the 50% BG solution was adjusted to 0.5% with respect to the total amount of the culture solution) was added instead of the extract of Production Example 1. The test plot (control plot) was set. After culturing for 24 hours, the cells were irradiated with ultraviolet rays (50 mJ / cm2), and after 24 hours, the cells in each test group were collected with 0.5 mL of Trizol reagent (manufactured by Invitrogen). 100 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the collected cells, stirred and mixed, and centrifuged at 15,000 rpm and 4 ° C. for 15 minutes with a centrifuge (TOMY Co., Ltd./MX-160). After that, only the aqueous layer was separated by 200 μL. 250 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer, stirred and mixed, and centrifuged at 15,000 rpm for 15 minutes under the conditions of 4 ° C. to obtain a total RNA precipitate. 1 mL of 75% ethanol was added to the total RNA, stirred and washed, and the precipitate was collected by centrifugation at 15,000 rpm for 15 minutes under the condition of 4 ° C. The recovered total RNA was reverse-transcribed using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Inc.)) to synthesize cDNA. Using the synthesized cDNA as a sample, using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.]. The expression of various genes and the expression of the internal standard substance G3PDH gene were detected. Here, G3PDH (glyceraldehyde-3-phosphate dehydrogenase) is a housekeeping gene (a gene that is commonly expressed in a certain amount in many tissues and cells, is always expressed, and is essential for cell maintenance and proliferation. It is one of the above, and since the expression level is always constant, it is used as an internal standard in PCR experiments. As for the test results, the expression level of each gene in each test group was compared when the expression level of the G3PDH gene was constant. In this test system, the relative value of the expression level of each gene in the other test group was determined when the expression level of each gene in the control group was set to 100. In Test Example 5, it is one of the proteins constituting the basal membrane at the boundary between the epidermis and the dermis, and it is used for gene expression of laminin involved in cell-cell adhesion and cell-cell adhesion by cell-cell adhesion factor (E-cadherin). The gene expression of β-catenin, which is a protein involved, and the gene expression of sphingomyelinase and basic ceramidese, which are enzymes related to ceramide metabolism, were evaluated.

The evaluation results of Test Example 5 are shown in Tables 3 and 4.
[Table 3]

First, as shown in Table 3, it was confirmed from the comparison results of the control group that the expression of the laminin gene and the β-catenin gene was reduced by ultraviolet irradiation. It is suggested that the extract of Production Example 4 has the effect of restoring the expression of the laminin gene and the β-catenin gene, which has been reduced by ultraviolet irradiation, strengthening the adhesion between cells, and adjusting the epidermal structure.

[Table 4]

First, as shown in Table 4, it was confirmed from the comparison results of the control group that the expression of the sphingomyelinase gene was reduced by ultraviolet irradiation. It was confirmed that the extract of Production Example 4 enhanced the expression of the sphingomyelinase gene, which was decreased by irradiation with ultraviolet rays. In addition, it was confirmed that the expression of the basic ceramidase gene was enhanced by irradiation with ultraviolet rays. It was confirmed that the extract of Production Example 4 suppressed the expression of the basic ceramidase gene enhanced by ultraviolet irradiation. These suggest that the extract of Production Example 4 has the effect of enhancing or suppressing the gene expression of the enzyme related to ceramide metabolism and suppressing the deterioration of the barrier function due to ultraviolet rays.

From the results in Tables 1 to 4, by combining the fermented product of the plant belonging to the genus Hibiscus of the Malvaceae according to the present invention and the extract of the plant of the genus Rose of the Rosaceae, the moisturizing property of the epidermis is maintained and the effect is reduced by ultraviolet rays. The structure of the epidermis can be strengthened, and as a result, the turnover of the epidermis can be normalized and the skin can be healthy.

Prescription example 1. Toner [Ingredients] Part Eucalyptus oil 0.2
Polyoxyethylene (5.5) cetyl alcohol 5.0
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Tocopherol acetate 0.02
Dipotassium glycyrrhizinate 0.5
Monoammonium glycyrrhizinate 0.5
Stearyl glycyrrhizinate 0.05
Isopropylmethylphenol 0.1
Aligned in 0.1
D-pantothenyl alcohol 0.1
Salicylic acid 0.5
Urea 5.0
l-menthol 0.9
dl-menthol 0.2
1,3-butylene glycol 5.0
Sodium citrate 0.2
Methylparaben 0.1
Hinokitiol 0.003
Photosensitizer No. 201 0.002
Amount of purified water totaling 100 parts

Prescription example 2. Toner [Ingredients] Part Caprylic acid glyceryl 3.0
Polyglyceryl laurate-10 3.0
Cetanol 2.0
Behenyl alcohol 2.0
Methylparaben 0.1
Fermented product of Production Example 2 2.0
Extract of Production Example 5 2.0
Ascorbic acid 3.0
Glycyrrhizic acid 0.5
β-Glycyrrhetinic acid 0.05
Tocopherol nicotinic acid ester 0.1
Resorcin 0.1
Zinc oxide 2.0
dl-camphor 0.5
Glycerin 2.0
1,3-butylene glycol 5.0
Potassium hydroxide 0.5
Amount of purified water totaling 100 parts

Prescription example 3. Toner use 2.0 parts of the fermented product of Production Example 3 is used in place of the fermented product of Production Example 2 contained in Formulation Example 2, and the extract of Production Example 6 is used in place of the extract of Production Example 5. Obtained a lotion in the same manner as in Formulation Example 2.

Prescription example 4. Toner [Ingredients] Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Methylparaben 0.1
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Ascorbic acid glucoside 2.0
Tranexamic acid 2.0
ε-aminocaproic acid 0.1
Sulfur 0.2
Estradiol 0.1
Glycerin 5.0
1,3-butylene glycol 5.0
Sodium citrate 0.2
Sodium metabisulfite 0.2
d-camphor 0.1
Amount of purified water totaling 100 parts

Prescription example 5. Emulsion [Ingredients] Part Squalene 5.0
Cyclopentane Siloxane 1.0
Hexalan 3.0
Hexyldecyl isostearate 1.0
Tri (caprylic acid / capric acid) glyceryl 1.0
Polyglyceryl laurate-10 5.0
Polyglyceryl isostearate-10 5.0
Ascorbyl dipalmitate 15.0
Hydrogenated soy lecithin 1.5
Fermented product of Production Example 1 1.0
Fermented product of Production Example 2 1.0
Extract of Production Example 4 1.0
Extract of Production Example 5 1.0
Ascorbic Acid Phosphate Magnesium Salt 3.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Xanthan gum 0.2
Snow fungus polysaccharide 0.2
Sodium hyaluronate 0.01
Tocopherol acetate 0.3
Tocopherol nicotinic acid ester 0.1
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Isopropylmethylphenol 0.1
Water-soluble collagen 1.0
Hydrolyzed collagen 1.0
Sodium hyaluronate 1.0
Amount of purified water totaling 100 parts

Prescription example 6. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 19 except that 2.0 parts of L-ascorbic acid-2-glucoside was used instead of 2.0 parts of ascorbic acid phosphate magnesium salt in the components of Formulation Example 5.

Prescription example 7. Emulsion In the same manner as in Formulation Example 19, except that 2.0 parts of tranexamic acid is used instead of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide in the components of Formulation Example 5. Emulsion was obtained.

Prescription example 8. Emulsion A milky lotion was obtained in the same manner as in Formulation Example 19 except that 3.0 parts of nicotinamide was used instead of 2.0 parts of ascorbic acid phosphate magnesium salt in the components of Formulation Example 5.

Prescription example 9. Cream [Ingredients] Part Olive oil 5.0
Jojoba oil 5.0
Squalene 5.0
Hexyldecyl isostearate 5.0
Di (octyldodecyl / phytosteryl / behenyl) 5.0
Glyceryl caprylate 1.0
Glyceryl stearate 1.0
Isostearyl glyceryl 3.0
γ-Oryzanol 0.1
Behenyl alcohol 2.0
Palmitic acid 2.5
D-Pantothenyl Alcohol 3.0
Allantoin 0.1
Riboflavin 0.01
Resorcin 0.1
Benzalkonium chloride 0.05
Urea 3.0
β-Glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Ammonium glycyrrhizinate 0.1
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Lactic acid bacteria fermented rice 2.0
Hydrogenated lecithin 0.5
Hydrogenated lysolecithin 0.5
Oil-soluble Panax ginseng extract 2.0
Xanthan gum 1.0
Zinc oxide 0.5
dl-camphor 0.3
l-menthol 0.5
Amount of purified water totaling 100 parts

Example 10. Pack [Ingredients] Part Dipropylene Glycol 5.0
Polyoxyethylene (60) hardened castor oil 5.0
Cetanol 3.0
Behenyl alcohol 3.0
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Ammonium glycyrrhizinate 0.1
β-Glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.1
Tocopherol acetate 0.5
Tocopherol nicotinic acid ester 0.1
D-pantothenyl alcohol 0.3
Resorcin 0.1
Sulfur 2.0
Estradiol 0.002
Fermented product of Production Example 1 1.0
Extract of Production Example 4 1.0
Xanthan gum 2.0
Polyglyceryl myristate-6 1.0
Potassium cocoyl glutamate 1.0
Hydrogenated lecithin 3.0
Lecithin Hydroxide 3.0
Amount of purified water totaling 100 parts

Prescription example 11. Hair shampoo [Ingredients] Part Sodium Laureth Sulfate 10.0
Glyceryl monostearate 1.0
Coconut oil fatty acid diethanolamide 2.0
Polyoxyethylene (40) hardened castor oil 0.5
Benzalkonium chloride 1.0
Stearyl alcohol 2.0
Behenyl alcohol 2.0
Dimethicone 3.0
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sodium salicylate 0.1
Tocopherol acetate 0.1
Pyrithione Zinc 0.3
Benzoic acid 0.2
Triclosan 0.2
Citric acid 0.1
Propylene glycol 2.0
Amount of purified water totaling 100 parts

Example 12. Hair Conditioner [Ingredients] Part Polyoxyethylene (10) Hardened Castor Oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Benzalkonium chloride 1.0
Cetanol 3.0
Stearyl alcohol 1.0
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Allantoin 0.1
Isopropylmethylphenol 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sulfur 0.5
Haloalkane bromide isoquinolinium solution (75%) 0.06
Pyrithione Zinc 0.3
Methylparaben 0.1
Triclosan 0.2
Resorcin 0.1
Amount of purified water totaling 100 parts

Prescription example 13. Cleaning cosmetics [Ingredients] Part Cocoyl glycine potassium 5.0
Glycerin 10.0
Glyceryl caprylate 1.0
Sodium lauroyl aspartate 10.0
Fermented product of Production Example 1 1.0
Fermented product of Production Example 2 1.0
Extract of Production Example 4 1.0
Extract of Production Example 6 1.0
Cetanol 3.0
Myristyl Alcohol 3.0
Isopropylmethyl alcohol 0.1
Allantoin 0.1
Sulfur 0.5
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Monoammonium glycyrrhizinate 0.1
β-Glycyrrhetinic acid 0.05
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.2
Tocopherol acetate 0.2
Triclosan 0.1
Trichlorocarbanide 0.5
Trichlorohydroxydiphenyl ether 0.2
Concentrated benzalkonium chloride solution 50 0.2
Benzalkonium chloride 0.1
Amount of purified water totaling 100 parts

Prescription example 14. Sheet mask A sheet mask is obtained by impregnating a non-woven fabric with the following components.
[Ingredients] Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
L-ascorbic acid 2-glucoside 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Xanthan gum 1.0
Water-soluble collagen 1.0
Sodium hyaluronate 1.0
Eelgrass extract 1.0
Rice extract hydrolyzate 1.0
Potassium hydroxide Appropriate amount Purified water The total amount is 100 parts

Prescription example 15. Essence [Ingredients] Part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid hydrolyzate 1.0
Lactic acid bacteria culture 1.0
Fermented product of Production Example 1 2.0
Extract of Production Example 4 2.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water totaling 100 parts

Claims (1)

An external preparation for the skin containing a fermented product of a plant belonging to the genus Confederate rose of the Malvaceae family and an extract of a plant belonging to the genus Rosaceae of the Rosaceae family.

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