JP2022078231A - Skin external preparations - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide active ingredients that are derived from natural products with excellent biological safety and promote uptake of vitamin C into a cell.

SOLUTION: Vitamin C uptake promoters contain as active ingredients one or more of: a fermented product of Nelumbonaceae, Nelumbo plants or extract thereof with lactic acid bacteria, yeast, koji mold or Bacillus subtilis; a hydrolysate of Poaceae, Oryza plants or extract thereof; extract of Chenopodiaceae, Salicornia plants; and a hydrolysate of soybean or extract thereof. The invention also provides skin external preparations or oral compositions containing these agents.

SELECTED DRAWING: None

COPYRIGHT: (C)2022,JPO&INPIT

Description

The present invention is a novel active ingredient derived from a natural product and having excellent bioactivity and biosafety, a skin (including scalp) external preparation containing the same, and an oral composition for beauty or health promotion. ..

In recent years, research has been conducted on cell aging and damage to cells caused by external factors (for example, ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and various cells have been studied. The mechanism of cell damage and its prevention and recovery has been elucidated.

Conventionally, active ingredients for preventing and improving the above-mentioned cell damage and aging phenomenon have been proposed, and cosmetics, foods and drinks and pharmaceuticals containing these active ingredients have been put on the market. For example, antioxidants such as vitamin C, vitamin E, catalase, or derivatives thereof; anti-inflammatory agents such as glycyrrhizinic acid or salts thereof, allantin, tranexamic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract. , Γ-Amino-β-hydroxybutyric acid and other cell-activating components; extracellular matrix components such as collagen, elastin, hyaluronic acid or salts thereof; moisturizing agents such as urea; and protein glycation inhibitor such as aminoguanidine.

However, these conventional components have problems in terms of stability, safety and effectiveness when actually applied to a living body. For example, the ascorbic acid or its derivative has a problem that it is easily oxidized by an external factor such as ultraviolet rays or an enzyme in a living body, and its uptake into cells decreases with aging.

As a result of diligent research to solve the above problems, the present inventors have found that fermented plants of the Nelumbonaceae (Nelumbonaceae) and Nelumbo (Nelumbo) plants, and hydrolyzates of plants of the Nelumbonaceae (Poaceae) and Oryza (Oryza) plants. An extract of a plant belonging to the genus Salicornia of the family Nelumbonaceae, and a hydrolyzate of soybean or its extract, is a vitamin C channel involved in the intracellular uptake of vitamin C, "Vitamin C transporter (Vitamin C transporter). It was also found that it has the effect of promoting the synthesis of SVCT).

Conventionally, an invention using a fermented product of the Lotuses family Lotuses, a hydrolyzate of a Gramineae plant, an extract of a plant of the Chenopodiaceae Salicornia, and a hydrolyzate of soybean or its extract as an external preparation for skin. Was known, for example, in Patent Documents 1-5. However, fermented lotus plants, gramineous oryza plant extracts or hydrolyzates, Chenopodiaceae Salicornia plant extracts, and soybeans or their extracts hydrolyzate synthesize SVCT1. It was not known to have a promoting effect.
JP 2005-298489 Japanese Patent Application Laid-Open No. 2002-128632 Japanese Patent Application Laid-Open No. 2013-103906 JP 2005-145878 JP 2006-290742

The present invention is a vitamin C uptake promoter containing a hydrolyzate of soybean or an extract thereof as an active ingredient.
The present invention is an external skin preparation containing the above-mentioned vitamin C uptake promoter.
The present invention is an oral composition containing the above-mentioned vitamin C uptake promoter.

Since the extract, hydrolyzate or fermented product according to the present invention can enhance the expression of SVCT-1, which is a vitamin C uptake port of pigment cells, it can be used as an active ingredient for whitening or antioxidants. When used in combination with vitamin C, it is possible to increase its uptake into pigment cells and obtain a more effective whitening effect.

Hereinafter, the present invention will be described in detail.
Examples of the lotus family (Nelumbonaceae) and Lotuses (Nelumbo) plants used in the present invention include Nelumbo nucifera and Nelumbo Lutea.

When a lotus plant is used to obtain a fermented product of a microorganism (yeast, lactic acid bacterium, aspergillus or bacillus), the fermentation site of the plant is not particularly limited, and the whole plant, leaves, flowers, stamens, female stamens, and stems are not particularly limited. , Rhizome, seeds (grains) and the like can be used as appropriate, but from the viewpoint of the effectiveness of the obtained fermented product, the use of whole plants or seeds is preferable.

Further, as the plant of the genus Oryza (Poaceae) used in the present invention, any plant belonging to Oryza sativa in terms of taxonomy can be used. Japanese barre, Akita Komachi, Kinuhikari, Hanaechizen, etc. can be mentioned, but of course, they are not limited to these. In the present invention, the site of use of the plant of the genus Oryza is not particularly limited, and an appropriate site such as whole plant, leaf, flower, stem, root, seed (grain) can be used, but the whole plant and leaf are used. preferable. When using leaves, it is more preferable to use leaves before heading. When rice leaves are used, the leaves may be heat-treated before the extraction treatment. As a result, it is possible to suppress denaturation and alteration due to active ingredients (enzymes and the like) contained in rice leaves, and it is possible to prevent a decrease in activity of the collected rice leaves during storage.

Examples of plants of the genus Salicornia of the family Chenopodiaceae include Salicornia, Salicornia europaea, Salicornia bigerovi, Salicornia doricostakia SP striktisima, and the like, but the present invention is not limited thereto. not. Examples of the sites where these plants are used include whole plants, flowers, leaves, stems, seeds and the like.

The soybean used as a material in the present invention is a plant classified into the same species as soybean (scientific name: Glycine max (L.) Merill) in terms of plant taxonomy. As the soybean, any of white soybean (yellow soybean), blue soybean, black soybean and the like can be used.

When preparing an extract from the above plants, for example, it can be carried out as follows. That is, first, the extraction site of each plant is washed with water in advance to remove foreign substances, if necessary, and then it is dried as it is or dried, and if necessary, it is shredded or crushed and brought into contact with an extraction solvent for extraction. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as a dipping method, but a supercritical extraction method or a steam distillation method can also be used.

Examples of the extraction solvent include water; lower alcohols such as methanol, ethanol and propanol, higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol; ethylene glycol, 1,3-propanediol and 1,3-butylene glycol. Polyhydric alcohols such as glycerin; esters such as ethyl acetate, butyl acetate, methyl propionate, glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether, isopropyl and ether; n-hexane and toluene , Hydrocarbon-based solvents such as chloroform, etc., and they can be used alone or in admixture of two or more.

When a mixed solvent is used, the mixing ratio is, for example, 1: 1 to 25: 1 in a volume ratio (hereinafter the same) in the case of a mixed solvent of water and ethyl alcohol, and 1: 1 in the case of a mixed solvent of water and glycerin. If it is 1 to 15: 1, or if it is a mixed solvent of water and 1,3-propanediol or 1,3-butylene glycol, the range is preferably 1: 1 to 15: 1.

When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkaline adjusting agent such as sodium hydroxide, sodium carbonate, potassium hydroxide or the like, or an acid adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid may be added to the extraction solvent, if desired. May be adjusted to the pH of.

Extraction conditions such as extraction temperature and extraction time differ depending on the type and pH of the solvent used, but for example, when water or 1,3-butylene glycol or a mixed solution of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C. to 90 ° C., and the extraction time is preferably 0.5 hour to 7 days.

Here, the extraction site may be subjected to a hydrolysis treatment, if necessary, prior to the extraction treatment of the present invention or in parallel with the extraction treatment. Thereby, there is a possibility that the extract can be used more effectively by improving the skin irritation, effectiveness, storage stability and the like of the extract.

In particular, in the present invention, it is preferable to hydrolyze the rice leaf and soybean extracts. Examples of the hydrolysis treatment method include a decomposition treatment method using an acid, an alkali or an enzyme.

When the hydrolysis treatment is performed using an enzyme, at least one enzyme selected from a proteolytic enzyme, a starch degrading enzyme, a pectin degrading enzyme, a fibrinolytic enzyme, and a lipid degrading enzyme can be used as the enzyme. ..

Examples of proteolytic enzymes include actinase such as actinase, pepsin such as pepsin, trypsin such as trypsin and chymotrypsin, papaine such as papaine and kimopapine, and peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase. , Bromeline, complex proteolytic enzyme derived from microorganisms (for example, neurase [manufactured by Amano Enzyme Co., Ltd.]) and the like can be used.

As the starch-degrading enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used.

As the pectin degrading enzyme, for example, pectinase, pectin depolymerizer, pectin demethoxylase, pectin lyase, pectin esterase, polygalacturonase and the like can be used.

As the fibrinolytic enzyme, for example, cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, caragenase, arginase, fucoidanase, inulase, xylanase, ligninase and the like can be used.

As the lipid-degrading enzyme, for example, lipase, phospholipase and the like can be used.

In addition, the present invention may be fermented with a microorganism (lactic acid bacterium, yeast, aspergillus, Bacillus subtilis, etc.) for the purpose of improving the effectiveness of the extracted material, suppressing skin irritation, or improving the stability. In particular, in the present invention, it is preferable to ferment a plant of the genus Lotuses or an extract thereof with a microorganism.

When fermentation is carried out, for example, it can be carried out as follows. First, as an assimilation source for fermentation, the plant itself (hereinafter, may be referred to as a plant) may be used, or an extract obtained by extracting the plant with an appropriate medium may be used. In addition, when an extract is used, it is also possible to carry out fermentation with the plant contained in the extract without removing the plant to be extracted by solid-liquid separation. Here, the plant may be raw or pre-dried or semi-dried. Moreover, it is also possible to use the collected one as it is as the shape.

In the present invention, the lactic acid bacterium is, for example, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, Lactobacillus delbrueckii, and the like. Lactobacillus genus Carnobacterium such as Carnobacterium divergens, Carnobacterium piscicola; Leuconostoc mesenteroides, Leuconostoc lactis, Leuko Lactobacillus of the genus Leuconostoc such as Nostock citreum (Leuconostoc citreum); Lactobacillus of the genus Streptococcus (Streptococcus pyogenes) such as Streptococcus faecalis (Streptococcus faecalis); Lactobacillus (Enterococcus) genus such as sulfreus; Lactococcus plantarum (Lactococcus plantarum) Lactococcus rafinolactis (Lactococcus rafinolactis) etc. Lactobacillus of the genus Vigococcus; Lactobacillus of the genus Atopobium such as Atopobium minutum, Atopobium parvulus; Vagococcus fluvialis, Vagococcus salmoninaram Lactic acid bacteria of the genus Vagococcus such as inarum; lactic acid bacteria of the genus Pediococcus such as Pediococcus damnosus and Pediococcus pentosaceus can be mentioned.

Further, in the present invention, yeast is, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces calsbergensis, Saccharomyces calsbergensis, etc. Saccharomyces yeast, Galactomyces yeast, Torulaspora delbruekii, Torulaspora fermentati, Torulaspora fermentati, Torulaspora rosei, etc. Zygosaccharomyces soya, Zygosaccharomyces sake, Zygosaccharomyces miso, Zygosaccharomyces miso, Zygosaccharomyces lactis yeast, Zygosaccharomyces lactis yeast, Zygosaccharomyces lactis Candida etchellsii), Candida kefyr, Candida sake, Candida scottii and other Candida yeasts, Aureobasidium Pullulans, Aureobasidium Examples thereof include yeasts of the genus Aureobasideium such as mansonii) and Aureobasideium microstictum. Further, the yeast according to the present invention may be any of sake yeast, wine yeast, beer yeast, yeast derived from plant flowers (rose, lily, cherry, etc.), and yeast derived from the sea.

Further, in the present invention, the aspergillus is, for example, Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus aspergillus, and Aspergillus aspergillus. ), Aspergillus kawauchii, Aspergillus usami, Aspergillus niger and other black aspergillus, Monascus anka, Monascus pilosus and other red aspergillus.

Further, in the present invention, the Bacillus subtilis includes, for example, Bacillus natto, Bacillus subtilis, Bacillus circulans and the like.

When the above-mentioned suspension or extract is fermented by microorganisms, it is necessary to perform sterilization to remove germs that hinder fermentation before the fermentation step. As a method for sterilizing and removing the germs, a method may be used in which the fermented material is previously washed with ethanol for sterilization and then suspended in a sterile solvent such as sterile water, or the fermented material is suspended in a solvent and then suspended. The turbid liquid may be sterilized by heat sterilization or the like. The heat sterilization treatment includes an autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, and intermittent sterilization in which the suspension is held at 80 to 90 ° C. for 60 to 120 minutes once a day for 2 to 3 days. A heat sterilization method such as a method is generally used.

The sterilized suspension is placed in a fermentation tank, in which microorganisms are inoculated and fermented. The appropriate amount of microorganisms to be inoculated is ¹⁰⁷ to ¹⁰⁸ cells / mL. Even if the inoculation amount is larger than the above range, the fermentation progress time is almost unchanged, while if it is smaller than the above range, it takes a long time to complete the fermentation, which is not preferable.

Fermentation temperature is generally in the range of 5 to 50 ° C, preferably 20 ° C to 40 ° C, which is the optimum temperature for growth of each microorganism (for example, 30 ° C to 40 ° C for lactic acid bacteria, 25 ° C to 30 ° C for yeast). Is the range of. The number of fermentation days is generally in the range of 1 to 10 days, preferably 2 to 5 days at the optimum temperature. When the number of fermentation days is shorter than the above general range, fermentation tends to be insufficient and the effectiveness of the fermented product tends to decrease, while even if it is longer than 10 days, no further increase in effectiveness is observed. Not only that, coloring and an increase in fermented odor occur, which is not preferable.

When preparing a fermented product from a plant of the genus Lotus, the suspension or the above-mentioned suspension before or at the same time as the inoculation of the microorganism is made so that the components of the target use site can be used more effectively as a source of assimilation of the microorganism. The above-mentioned hydrolysis treatment may be performed on the extract solution.

The extract, hydrolyzate or fermented product prepared as described above may generally have a pH adjusted to 3 to 9 and then used as it is, or may be used as it is, or may have a desired concentration by concentration under reduced pressure or the like. May be used as. Further, it may be a dried product by a conventional method such as a spray drying method.

Further, the extract, hydrolyzate or fermented product prepared as described above may be stored in a refrigerator for a certain period of time and then the supernatant may be used in order to improve storage stability and the like.

The extract, hydrolyzate or fermented product according to the present invention may be added to skin external preparations (cosmetics, quasi-drugs, external pharmaceuticals), cosmetic or health-promoting foods and drinks (oral compositions). can. Examples of external skin preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, make-up press powders, cheeks, white powder, wash pigments, body shampoos, scalp, hair shampoos, and hair conditioners. , Shampoo or tonic for hair growth and hair growth, cleansing cosmetics such as soap, and bathing agents, but the present invention is not limited thereto. In addition, as foods and drinks for beauty or health promotion, beverages such as beauty beverages, nutritional drinks, sports drinks, near water, vitamin beverages, mineral beverages, alcoholic beverages; various soups (including powdered soups), dairy products , Jelly, candy, tablet confectionery, gum and other foods; can be blended in tablets, liquids, granules or jelly-like health foods / beverages, etc. Can be blended in products, etc.

The blending amount of the extract, hydrolyzate or fermented product according to the present invention can be appropriately adjusted depending on the formulation to be blended, and the solid content amount is, for example, 0.002 to 1.0 in the case of basic cosmetics. In the range of% by weight (solid content weight%, the same applies hereinafter), in the case of make-up cosmetics, in the range of 0.002 to 1.0% by weight, and in the case of cleansing cosmetics, 0.002 to 10.0. It is in the range of% by weight. In the case of hair cosmetics, the solid content of the composition is in the range of 0.0001 to 5.0% by weight. The amount to be blended in the oral composition is preferably in the range of 0.1 to 15% by weight as the solid content.

In addition to the essential ingredient extracts, hydrolysates or fermented products, the formulations containing the extracts, hydrolysates or fermented products according to the present invention include components usually used for external skin preparations and oral compositions. For example, oily ingredients, surfactants (synthetic, natural products), moisturizers, thickeners, preservatives / bactericides, powder ingredients, antioxidants, chelating agents, pH regulators, pigments, fragrances, etc. are required. Can be appropriately blended according to the above. In addition, it may be used in combination with other physiologically active ingredients as long as its effectiveness and features are not impaired.

Here, examples of the oily component include lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, palm oil, chamomile oil, palm oil, cacao oil, and meadowfoam oil. Plant-derived oils and fats such as rosehip oil, lambender oil, sheer butter, tea tree oil, avocado oil, macadamia nut oil, and plant-derived squalane; animal-derived oils and fats such as mink oil and turtle oil; Rows such as wax and lanolin; hydrocarbons such as liquid paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and eicosenoic acid; lauryl alcohol, cetanol, Higher alcohols such as stearyl alcohol; examples thereof include synthetic esters such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene hydrogenated castor oil. Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates , Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates and other anionic surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N , N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamide propyl-N', N'-dimethyl-N' An amphoteric surfactant such as -β-hydroxypropylammoniosulfobetaine can be used.

Examples of the emulsifier or emulsifying aid include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, and water-soluble soybeans. Polysaccharides, soybean-derived protein-polysaccharide complex, lanolin or its derivatives, cholesterol, stevia derivatives (stevia enzyme treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and theirs. Derivatives, cholesterol and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented sprouted rice, lactic acid bacteria fermented grains (wheat, beans, miscellaneous grains, etc.) and the like can also be blended.

Examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, and saccharides such as trehalose and mucopolysaccharides (for example, for example). Hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane roots (white and white) ) Extracts, various amino acids and their derivatives.

Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, components derived from green algae or red algae; silane root (white and) extract; pectin, locust bean gum, aloe polysaccharide, alkaligenes-producing polysaccharide and the like. Polysaccharides; gums such as xanthan gum, tragant gum, guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, alginic acid / methacrylic acid copolymer; Acids and derivatives thereof; examples thereof include polyglutamic acid and derivatives thereof.

Examples of antiseptic / bactericidal agents include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, and chloride. Bensalconium, salicylic acid, ethanol, undesicylene acid, phenols, jamar (imidazole dainie ruurea), polyphosphate, propanediol, 1,2-pentanediol, various essential oils, dried bark, radish fermented liquid, sugar cane, etc. There are plant-derived ethanol or 1,3-butylene glycol and the like.

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic acid anhydride, mica, nylon powder, polyethylene powder, silk powder, and the like. There are cellulose-based powders, grain (rice, wheat, corn, millet, etc.) powders, beans (soybeans, small beans, etc.) powders, and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzone, 2,4-dihydroxybenzophenone, and 2-hydroxy-. 4-methoxybenzophenone-5-sulfonate, 4-tertiary butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. be.

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, beautyberry extract, silane root extract, shakuyaku extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate). Etc.) etc.

Examples of the chelating agent include ethylenediamine hydroxyethyl trisodium triacetate, edetonic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyetanediphosphonate and the like.

Examples of the pH adjuster include citric acid or salts thereof, lactic acid or salts thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like.

Examples of the whitening agent include t-cycloaminomino acid derivatives, kodiic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives. , 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine mono) Phosphate, adenosine monophosphate) may be mentioned, and these may be blended alone or in combination of two or more.

Examples of the succinic acid derivative include succinic acid esters such as succinic acid monobutyrate, succinic acid monocaplate, succinic acid monopalmitate, and succinic acid dibutyrate, and succinic acid sugar derivatives such as succinic acid ethers and succinic acid glucoside. As the ascorbic acid derivative, for example, L-ascorbic acid-2-phosphate ester sodium, L-ascorbic acid-2-phosphate ester magnesium, L-ascorbic acid-2-sulfate ester sodium, L-ascorbic acid- Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorvirtocopheryl maleic acid, ascorvirtocopheryl phosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyle group, etc.), L-ascorbic acid tetraisopalmitic acid ester, L. -L-ascorbic acid tetra fatty acid esters such as ascorbic acid tetralauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acyl Glucerin ascorbic acid derivatives such as chemical derivatives, bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate. As the hydroquinone derivative, albutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as the tranexamic acid derivative, tranexamic acid ester (for example, tranexamic acid lauryl ester). , Tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, tranexamic acid methylamide) and the like, and examples of the resorcinol derivative include 4-n-butylresorcinol and 4-isoamyl. Examples of the 2,5-dihydroxybenzoic acid derivative such as resorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyl. Oxybenzoic acid and the like, nicotinic acid derivatives include, for example, nicotinic acid amide, benzyl nicotinate and the like, and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like. ..

Examples of the physiologically active ingredient include placenta extract, soybean extract, yukinoshita extract, perilla extract, rice bran extract or its hydrolyzate, white mustard extract or its hydrolyzate, fermented white mustard, and shakyaku. Extract or its hydrolyzate, lactic acid bacterium fermented rice, purple kib extract, lotus flower extract, honeybee hydrolyzate, honeybee seed fermented product, royal jelly fermented product, liquor lees extract or ceramide contained therein, liquor lees fermented product, Examples include Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. In addition, eggplant (has, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolyzate, apricot fruit extract, seaweed extract such as katamenkirinsai, marine flowering plant extract such as amamo, Fermented soymilk, jellyfish water, rice extract or its hydrolyzate, fermented rice extract, sprouted rice extract or its hydrolyzate, sprouted rice fermented product, black bean extract or its hydrolyzate, damask rose flower extraction Substances, extracts of bamboo shoot skin, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid, etc.), collagen and its derivatives derived from animals or fish, elastin and its derivatives, glycyrrhizinic acid and its derivatives (dipotassium salt, etc.) ), T-cycloaminoamino acid derivative, vitamin A and its derivatives, allantin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, otane carrot extract or fermented product thereof, Red ginseng extract, fermented honey extract, hechima extract, anaaosa extract, peach extract, peach seed extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, paudalco bark extract, hibiscus flower extract Fermented product or fermented product, Hagoromogusa extract, Cherimoya extract, Mango extract, Mangostin extract, Funori extract, Karyucha extract, Benifuki extract, Silane extract, Sansho peel or seed coat extract or hydrolyzate, Benibana flower extract, Casablanca extract, sweet potato extract or fermented product thereof, Guava leaf extract, Dokudami extract, late white yuzu extract, aloe extract, fig flower extract, apple extract, white asparagus extract And so on.

Next, the present invention will be described in more detail with reference to production examples, test examples, and prescription examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Fermented product solution of lotus seeds (1)
100 g of lotus seeds (without astringent skin) were crushed, 1900 g of purified water was added to prepare a suspension, and the suspension was sterilized by heating. This suspension was inoculated with ¹⁰⁸ lactic acid bacteria (Lactobacillus plantarum) / mL and statically cultured at 37 ° C. for 3 days under a nitrogen stream. After completion of the culture, the cells were sterilized by heating, and the culture solution was filtered to obtain 1415 g (solid content concentration 2.53%) of a fermented lactic acid bacterium solution of lotus seeds.

Production example 2. Fermented product solution of lotus seeds (2)
As a microorganism, 1430 g (solid content concentration 2.24%) of a yeast fermented bean seed solution was obtained in the same manner as in Production Example 1 except that Saccharomyces cerevisiae, which is a yeast, was used instead of lactic acid bacteria (Lactobacillus plantarum).

Production example 3. Fermented product solution of lotus seeds (3)
Aspergillus oryzae, which is a Jiuqu bacterium, was used as the bacterium instead of the lactic acid bacterium (Lactobacillus plantarum). ..

Production example 4. Fermented product solution of lotus seeds (4)
As the microorganism, 1385 g (solid content concentration 2.60%) of Bacillus subtilis fermented solution of Hasseed seeds was used in the same manner as in Production Example 1 except that Bacillus natto, which is a Bacillus subtilis, was used instead of lactic acid bacteria (Lactobacillus plantarum). Obtained.

Production example 5. Whole plant fermented product solution of lotus (1)
As the fermentation material, 1167 g (solid content concentration 1.21) of a fermented lactic acid bacterium solution of whole plant of Nelumbo nucifer was used in the same manner as in Production Example 1 except that a shredded product of 100 g of whole plant of Nelumbo nucifer was used instead of the crushed product of whole plant of Nelumbo nucifer. %) Was obtained.

Production example 6. Rice leaf hydrolyzate solution (1)
1000 g of purified water is added to 200 g of dried crushed rice leaves immediately before heading (earing stage), extraction is performed at 80 ° C. for 1 hour, and then filtration is performed to 550 g (solid) of a pale yellow transparent rice leaf extract solution. Mineral concentration 2.5%) was obtained. 0.025 g of pectinase was added to 500 g of the obtained extract solution, and the mixture was hydrolyzed at 40 ° C. for 4 hours. Then, the enzyme was inactivated by heating at 90 ° C. for 1 hour and then filtered to obtain 455 g (solid content concentration 2.72%) of an enzyme hydrolyzate solution of a pale yellow transparent rice leaf extract.

Production example 7. Rice leaf hydrolyzate solution (2)
In the same manner as in Production Example 1 except that cellulase was used instead of pectinase, 450 g (solid content concentration 2.31%) of an enzyme hydrolyzate solution of rice leaf extract was obtained.

Production example 8. Rice leaf hydrolyzate solution (3)
In the same manner as in Production Example 1 except that xylanase was used instead of pectinase, 452 g (solid content concentration 2.43%) of an enzyme hydrolyzate solution of rice leaf extract was obtained.

Production example 9. Preparation of Hamcho extract (1)
200 g of purified water was added to 20 g of dried shredded whole plant of Salicornia herbacea, and the mixture was extracted at 40 ° C. for 1 hour. The obtained extract was filtered to obtain 155 g (solid content: 2.00%) of a brown transparent extract solution.

Production example 10. Preparation of Hamcho extract (2)
200 g of purified water was added to 20 g of dried shredded Hamcho stems, and the mixture was extracted at 40 ° C. for 3 hours. The obtained extract was filtered to obtain 152 g of a brown transparent extract solution (solid content: 1.99%).

Production example 11. Preparation of Hamcho extract (3)
200 g of a 50% 1,3-butylene glycol aqueous solution was added to 20 g of dried shredded Hamcho whole plants, and the mixture was extracted at 40 ° C. for 5 hours. The obtained extract was filtered to obtain 160 g of a light brown transparent extract solution (solid content: 1.95%).

Production example 12. Soybean hydrolyzate 200 g of purified water was added to 10 g of a dried and ground soybean seed (black soybean), and the mixture was extracted at 80 ° C. for 1 hour. Neulase (manufactured by Amano Enzyme Co., Ltd.) was added to the obtained extract roughly filtered to a concentration of 0.01%, and the mixture was allowed to act at 40 ° C. for 3 hours. Next, the mixture was treated at 80 ° C. for 1 hour to inactivate the enzyme and then filtered to obtain 160 g of a light brown transparent black soybean extract hydrolyzate solution (solid content concentration 1.2%).

Prescription example 1. Emulsion [Ingredients] Part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Hass essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 1.0
Oil-based glyceryl stearate 1.0
Hydrogenated soy lecithin 1.5
Fermented product of Production Example 1 3.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Appropriate amount of fragrance Amount of purified water that makes 100 copies

Prescription example 2. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 3.0 parts of the fermented product of Production Example 2 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 3. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 3.0 parts of the fermented product of Production Example 3 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 4. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 3.0 parts of the fermented product of Production Example 4 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 5. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 3.0 parts of the fermented product of Production Example 5 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 6. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the hydrolyzate of Production Example 6 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 7. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the hydrolyzate of Production Example 7 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 8. Emulsion An emulsion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the hydrolyzate of Production Example 8 was used in place of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 9. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the extract of Production Example 9 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 10. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the extract of Production Example 10 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 11. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the extract of Production Example 11 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 12. Emulsion A milky lotion was obtained in the same manner as in Formulation 1 except that 5.0 parts of the hydrolyzate of Production Example 12 was used instead of 3.0 parts of the fermented product of Production Example 1 contained in Formulation Example 1.

Prescription example 13. Emulsion Prescription except that 2.0 parts of L-ascorbic acid-2-phosphate ester magnesium is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 1. A milky lotion was obtained in the same manner as in Example 1.

Prescription example 14. Emulsion Prescription except that 2.0 parts of L-ascorbic acid-2-phosphate ester sodium is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 1. A milky lotion was obtained in the same manner as in Example 1.

Prescription example 15. Emulsion Same as Formulation Example 1 except that 1.0 part of 3-O-ethylascorbic acid is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 1. And obtained milky lotion.

Prescription example 16. Emulsion [Ingredients] Part Squalene 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 1.0
Glycerin fatty acid ester 1.0
Soy lecithin 1.5
Fermented product of Production Example 1 5.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Amount of purified water that makes 100 copies

Prescription example 17. Emulsion An emulsion was obtained in the same manner as in Prescription Example 16 except that 1.0 part of tranexamic acid was used instead of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 16.

Prescription example 18. Toner [Ingredients] Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Fermented product of Production Example 1 5.0
L-ascorbic acid 2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide Appropriate amount Fragrance Appropriate amount Purified water 100 parts in total

Prescription example 19. Lotion [Ingredients] Part Production Example 2 Fermented product 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Appropriate amount of fragrance Potassium hydroxide Appropriate amount Purified water 100 parts in total

Prescription example 20. Lotion A lotion was obtained in the same manner as in Formulation 19 except that 10.0 parts of the hydrolyzate of Production Example 6 was used in place of the fermented product of Production Example 2 contained in the components of Formulation Example 19.

Prescription example 21. Essence [Ingredients] Part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid solution 0.1
Extract of Production Example 9 5.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water that makes 100 copies

Example 22. Liquid Foundation [Ingredients] Part Stearic Acid 2.4
Propylene glycol monostearate 2.0
Setostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Fermented product of Production Example 4 5.0
Sodium Carboxymethyl Cellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Amount of purified water totaling 100 parts Titanium oxide 8.0
Talc 4.0
Appropriate amount of coloring pigment

Prescription example 23. Body shampoo [Ingredients] Part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Hydrolyzed product of Production Example 7 5.0
1,3-butylene glycol 2.0
Amount of purified water that makes 100 copies

Prescription example 24. Hair Shampoo [Ingredients] Part N-Palm Oil Fatty Acid Methyl Taurine Sodium 10.0
Polyoxyethylene (3) Alkyl Ether Sodium Sulfate 20.0
Betaine Lauryldimethylaminoacetic Acid 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Extract of Production Example 10 2.0
1,3-butylene glycol 2.0
Amount of purified water that makes 100 copies

Prescription example 25. Hair conditioner [Ingredients] Part Polyoxyethylene (10) Hardened castor oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyl trimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Hydrolyzed product of Production Example 8 2.0
1,3-butylene glycol 5.0
Amount of purified water that makes 100 copies

Prescription example 26. Beverage [Ingredients] Hydrolyzed product of Production Example 6 5.0
Citric acid 0.1
Sweetener (sucrose) 0.01
Vitamin C 0.1
Amount of purified water that makes 100 copies

Prescription example 27. Beverage A beverage was obtained in the same manner as in Formulation 26 except that the hydrolyzate of Production Example 9 was used in place of the fermented product of Production Example 1 contained in Formulation Example 26.

Prescription example 28. Beverage A beverage was obtained in the same manner as in Formulation 26 except that the hydrolyzate of Production Example 12 was used in place of the fermented product of Production Example 1 contained in Formulation Example 26.

Test example 1. Sodium-dependent vitamin C transporter (SVCT) synthesis promoting effect Normal human epidermal melanocytes were prepared in 1 x 10 ⁵ cells / mL with DermaLife® [registered trademark] containing growth additives, and 96-well microplates. 100 μL each was seeded and cultured at 37 ° C. under 5% carbon dioxide gas and saturated steam. After 24 hours, a culture solution containing the fermented products of Production Examples 1 and 2, the hydrolysates of Production Examples 6 and 7, and the extracts of Production Examples 9 and 10 as sample solutions 1 was added and further cultured. Here, the sample solution 1 was prepared so that the final concentrations of the solution with respect to the culture solution were 0.25%, 0.5%, and 1.0%. Further, the hydrolyzate of Production Example 12 was used as the sample solution 2 and prepared so that the final concentration as a solution with respect to the culture solution was 2.0%. In addition, as controls, control group 1 to which a culture solution containing PBS (-) (1.0%) was added instead of sample solution 1 and PBS (-) (2.0%) were contained instead of sample solution 2. The control group 2 to which the culture solution was added was set. After 48 hours, the culture supernatant was removed, 200 μL of PBS (-) was added to remove it, and then 50 μL of 10% trichloroacetic acid (Wako Pure Chemical Industries, Ltd.) was added and incubated for 30 minutes at cold temperature. After that, the supernatant was removed. The cells were washed with 100 μL of PBS (-), 50 μL of PBS (-) containing 0.2% Triton-X was added, and the mixture was incubated at room temperature for 1 hour. After removing the supernatant, 50 μL of PBS (-) containing 8% bovine serum albumin (SIGMA) was added and incubated at room temperature for 2 hours. The supernatant was removed and washed with 100 μL of PBS (-) containing 0.2% Triton-X, and 50 μL of anti-SVCT mouse monoclonal antibody (Santa Cruz) was added and incubated for 24 hours at cold temperature. The supernatant was removed and washing was repeated 3 times with 100 μL of PBS (-) containing 0.2% Triton-X. Alexa Fluor 488 anti-mouse secondary antibody (Life Technologies) was added in an amount of 50 μL and incubated at room temperature for 2 hours in the dark. Remove the supernatant, repeat washing 3 times with 100 μL of PBS (-) containing 0.2% Triton-X, add 100 μL of PBS (-) at a time, and use a fluorescent plate reader (Dainippon Pharmaceutical Co., Ltd.) to Ex485 / Em520. The fluorescence intensity in was measured. The relative value of the fluorescence intensity with respect to the measured value in the control group was taken as the SVCT synthesis rate (%).

Table 1 shows the results of Test Example 1 regarding the sample solution 1.
[Table 1]

As shown in Table 1, it was confirmed that the extract, hydrolyzate or fermented product according to the present invention has a concentration-dependently excellent SVCT synthesis promoting action.

Table 2 shows the results of Test Example 1 regarding the sample solution 2.
[Table 2]

As shown in Table 2, it was confirmed that the hydrolyzate according to the present invention has a remarkably excellent SVCT synthesis promoting action.

Claims (1)

A vitamin C uptake promoter containing a hydrolyzate of soybean or its extract as an active ingredient.